CN107033118A - The extracting method of OPC - Google Patents
The extracting method of OPC Download PDFInfo
- Publication number
- CN107033118A CN107033118A CN201710257567.0A CN201710257567A CN107033118A CN 107033118 A CN107033118 A CN 107033118A CN 201710257567 A CN201710257567 A CN 201710257567A CN 107033118 A CN107033118 A CN 107033118A
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- China
- Prior art keywords
- opc
- extracting method
- powder
- mixed
- mixed extract
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of extracting method of OPC, comprise the following steps：(1), take and clean grape pip and Grape Skin after drying, in proportion 12 15:26, mix, be broken into powder；(2), step (1) powder, is added into mixed extract, mixed extract is by volume ratio 12 16:6‑9:35 ethanol, ethyl acetate and water, which is mixed, to be constituted；Stir；(3), (2) step mixes material, at 8 10MPa, 15 22 DEG C, and with 3 3.5MHz ultrasonic extraction 30 50 minutes, quickly cooling to room temperature, filtering, filter vacuum is dried.The present invention is using the waste material grape pip and Grape Skin that can not directly eat, and as OPC preparing raw material, twice laid is cost-effective；Under mixed solvent effect, be conducive to the dissolving of OPC, and ultrasound is extracted under high pressure, low temperature, OPC is not oxidizable, and extraction efficiency is high, and impurity is few, and speed is fast.
Description
Technical field
The present invention relates to technical field of biological extraction, especially a kind of extracting method of OPC.
Background technology
OPC, English name is Oligomeric Proantho Cyanidins（OPC）, it is that one kind has special point
The bioflavonoid of minor structure, is that the maximally effective natural of removing people's interior free yl generally acknowledged in the world at present has
Very strong activity in vivo, it is demonstrated experimentally that OPC Green Tea Extract oxidability is 50 times of vitamin E, ascorbic 20 times,
And absorb rapid complete.Its preparation approach is typically the extraction via grape pip, is extracted using water extraction or organic solvent.
Due to the high antioxidant of OPC, rapidly applied in cosmetics, in all kinds skin care item
Antioxidant is added, to improve protective effect of the product to skin；And at present there is recovery rate not in the extracting method of OPC
The high shortcoming of high, impurity content, causes the cosmetics containing OPC expensive, it is impossible to obtains promoting on a large scale and answers
With.
The content of the invention
The present invention in view of the shortcomings of the prior art, proposes a kind of extracting method of OPC, and recovery rate is high, impurity content
It is low.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：A kind of extracting method of OPC, bag
Include following steps：
(1), take and clean grape pip and Grape Skin after drying, in proportion 12-15:2-6, mixes, is broken into powder；
(2), step (1) powder, is added into mixed extract, mixed extract is by volume ratio 12-16:6-9:3-5 ethanol, second
Acetoacetic ester and water, which are mixed, to be constituted；Stir；
(3), (2) step mixes material, at 8-10MPa, 15-22 DEG C, with 3-3.5MHz ultrasonic extraction 30-50 minutes, quickly cooling
To room temperature, filtering, filter vacuum is dried.
Further, (3) middle extraction process is carried out step in the case of starvation.
Further, extraction process is carried out in an inert atmosphere.
Compared with prior art, the present invention has advantages below：Using the waste material grape pip and grape that can not directly eat
Skin, as OPC preparing raw material, twice laid is cost-effective；Under mixed solvent effect, be conducive to the molten of OPC
Solution, and ultrasound is extracted under high pressure, low temperature, OPC is not oxidizable, and extraction efficiency is high, and impurity is few, and speed is fast.
Embodiment
With reference to embodiment, the present invention will be described in detail, the description of this part be only it is exemplary and explanatory, no
Reply protection scope of the present invention has any restriction effect.
Embodiment 1
A kind of extracting method of OPC, comprises the following steps：
(1), take and clean grape pip and Grape Skin after drying, in proportion 14:4, mix, be broken into powder；
(2), step (1) powder, is added into mixed extract, mixed extract is by volume ratio 14:8:4 ethanol, ethyl acetate
Mix and constitute with water；Stir；
(3), (2) step mixes material, at 9MPa, 19 DEG C, under carbon dioxide environment, with 3.2MHz 35 points of ultrasonic extraction
Clock, quickly cooling to room temperature, filtering, filter vacuum is dried.
Liquid chromatographic detection is carried out to product, relative amount is 80%.
Embodiment 2
A kind of extracting method of OPC, comprises the following steps：
(1), take and clean grape pip and Grape Skin after drying, in proportion 12:6, mix, be broken into powder；
(2), step (1) powder, is added into mixed extract, mixed extract is by volume ratio 12: 9:3 ethanol, ethyl acetate
Mix and constitute with water；Stir；
(3), (2) step mixes material, at 10MPa, 15 DEG C, under helium environment, with 3.5MHz ultrasonic extraction 30 minutes, speed
It is cooled to room temperature, filters, filter vacuum is dried.
Liquid chromatographic detection is carried out to product, relative amount is 82%.
Embodiment 3
A kind of extracting method of OPC, comprises the following steps：
(1), take and clean grape pip and Grape Skin after drying, in proportion 15:2, mix, be broken into powder；
(2), step (1) powder, is added into mixed extract, mixed extract is by volume ratio 16:6:5 ethanol, ethyl acetate
Mix and constitute with water；Stir；
(3), (2) step mixes material, at 8MPa, 22 DEG C, under starvation environment, with 3MHz ultrasonic extraction 50 minutes,
Quickly cooling to room temperature, filtering, filter vacuum is dried.
Liquid chromatographic detection is carried out to product, relative amount is 83%.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. a kind of extracting method of OPC, comprises the following steps：
(1), take and clean grape pip and Grape Skin after drying, in proportion 12-15:2-6, mixes, is broken into powder；
(2), step (1) powder, is added into mixed extract, mixed extract is by volume ratio 12-16:6-9:3-5 ethanol, second
Acetoacetic ester and water, which are mixed, to be constituted；Stir；
(3), (2) step mixes material, at 8-10MPa, 15-22 DEG C, with 3-3.5MHz ultrasonic extraction 30-50 minutes, quickly cooling
To room temperature, filtering, filter vacuum is dried.
2. the extracting method of OPC as claimed in claim 1, it is characterised in that：Step (3) middle extraction process in starvation
In the case of carry out.
3. the extracting method of OPC as claimed in claim 2, it is characterised in that：Extraction process enters in an inert atmosphere
OK.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710257567.0A CN107033118A (en) | 2017-04-19 | 2017-04-19 | The extracting method of OPC |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710257567.0A CN107033118A (en) | 2017-04-19 | 2017-04-19 | The extracting method of OPC |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107033118A true CN107033118A (en) | 2017-08-11 |
Family
ID=59535509
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710257567.0A Pending CN107033118A (en) | 2017-04-19 | 2017-04-19 | The extracting method of OPC |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107033118A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111848567A (en) * | 2020-07-15 | 2020-10-30 | 陕西源邦生物技术有限公司 | Method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn |
-
2017
- 2017-04-19 CN CN201710257567.0A patent/CN107033118A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 吕玉姣等: "原花青素提取、分离纯化方法研究进展", 《化学世界》 * |
| 张文叶等: "超高压提取葡萄籽中原花青素的研究", 《第五届国际葡萄与葡萄酒学术研讨会》 * |
| 汪振洋: "超声波辅助提取葡萄籽中原花青素", 《中国优秀硕士学位论文工程科技1辑东北林业大学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111848567A (en) * | 2020-07-15 | 2020-10-30 | 陕西源邦生物技术有限公司 | Method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170811 |
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| RJ01 | Rejection of invention patent application after publication |