CN111802513A - Preparation method of glucose oxidase probiotic additive for poultry livestock feed - Google Patents
Preparation method of glucose oxidase probiotic additive for poultry livestock feed Download PDFInfo
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- CN111802513A CN111802513A CN201910288195.7A CN201910288195A CN111802513A CN 111802513 A CN111802513 A CN 111802513A CN 201910288195 A CN201910288195 A CN 201910288195A CN 111802513 A CN111802513 A CN 111802513A
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- 108010015776 Glucose oxidase Proteins 0.000 title claims abstract description 33
- 239000004366 Glucose oxidase Substances 0.000 title claims abstract description 33
- 229940116332 glucose oxidase Drugs 0.000 title claims abstract description 33
- 235000019420 glucose oxidase Nutrition 0.000 title claims abstract description 33
- 239000006041 probiotic Substances 0.000 title claims abstract description 26
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 19
- 244000144972 livestock Species 0.000 title claims abstract description 17
- 239000000654 additive Substances 0.000 title claims abstract description 15
- 230000000996 additive effect Effects 0.000 title claims abstract description 15
- 244000144977 poultry Species 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 239000000243 solution Substances 0.000 claims abstract description 23
- 239000002131 composite material Substances 0.000 claims abstract description 14
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 10
- 239000000047 product Substances 0.000 claims abstract description 10
- 241000499912 Trichoderma reesei Species 0.000 claims abstract description 9
- 239000011550 stock solution Substances 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 12
- 244000061456 Solanum tuberosum Species 0.000 claims description 11
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 8
- 108010080698 Peptones Proteins 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- 235000019319 peptone Nutrition 0.000 claims description 8
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 8
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 235000015099 wheat brans Nutrition 0.000 claims description 8
- 229930003451 Vitamin B1 Natural products 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 235000012015 potatoes Nutrition 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 229960003495 thiamine Drugs 0.000 claims description 6
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 6
- 239000011691 vitamin B1 Substances 0.000 claims description 6
- 235000010374 vitamin B1 Nutrition 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 239000002518 antifoaming agent Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 abstract description 6
- 238000011160 research Methods 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 239000002552 dosage form Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000012467 final product Substances 0.000 abstract 1
- 239000003674 animal food additive Substances 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000036528 appetite Effects 0.000 description 2
- 235000019789 appetite Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Husbandry (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
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Abstract
The invention discloses a preparation method of a glucose oxidase probiotic additive for poultry livestock feed, which comprises the following preparation steps: A. preparing a composite flora raw material: (1) taking clostridium butyricum stock solution to fully propagate and activate in warm solution with sufficient carbon source for standby; (2) simultaneously, carrying out mother culture of trichoderma reesei, inoculating trichoderma reesei blocks to a PDA culture medium, and carrying out dark culture at 25 ℃ until hyphae grow over an inclined plane for later use; B. preparing a first-stage liquid strain; C. preparing a secondary liquid strain; D. adding glucose oxidase to obtain the final product. Based on the current situation of production research of the applicant unit, the invention is combined with local colleges and universities to research and develop a compound biological probiotic + glucose oxidase compound preparation with a brand-new dosage form, and the obtained new product has good practical value and brings a brand-new direction for the development of local industries.
Description
Technical Field
The invention relates to the technical field of livestock breeding, in particular to an additive for livestock feed.
Background
At present, various feed additives are common in the livestock artificial breeding industry, but the research and development of high-end functionalized multi-effect feed additives, particularly complex microbial inoculum and enzyme preparation products based on modern biological science, are still short boards in the industry, and have to be overcome and developed.
Disclosure of Invention
The invention aims to provide a preparation method of a glucose oxidase probiotic additive for poultry livestock feed.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows.
The preparation method of the glucose oxidase probiotic additive for poultry livestock feed comprises the following preparation steps:
A. preparing a composite flora raw material:
(1) taking clostridium butyricum stock solution to fully propagate and activate in warm solution with sufficient carbon source for standby;
(2) simultaneously, carrying out mother culture of trichoderma reesei, inoculating trichoderma reesei blocks to a PDA culture medium, and carrying out dark culture at 25 ℃ until hyphae grow over an inclined plane for later use;
B. preparing a first-stage liquid strain:
(1) preparing a culture solution; the culture solution comprises the following components in parts by weight: 10-15 parts of potato, 1-1.5 parts of brown sugar, 1-1.5 parts of glucose, 3-4 parts of wheat bran, 1-2 parts of peptone, 1-2 parts of monopotassium phosphate, 1-1.5 parts of magnesium sulfate, 1 part of agar and 75-80 parts of clear water, wherein the pH value is natural; adding vitamin B1 into the culture solution;
(2) placing the prepared culture solution into a triangular flask, sealing, sterilizing, cooling to below 25 deg.C, inoculating the mycelia of the mother strain obtained in step A, and inoculating 1-2 mycelia of the mother strain with length and width of 2mm into each 100ml of the culture solution; simultaneously adding 10-50ml of clostridium butyricum activation propagation liquid obtained in the step A; mixing the strains, and culturing for 1-2.5 days to obtain first-stage liquid strain;
C. preparing a secondary liquid strain:
(1) adding 90-100 parts by weight of water into 9-11 parts by weight of potatoes, boiling until the potatoes are crisp but not rotten, adding 3.4-3.8 parts by weight of large wheat bran, filtering by using 6-8 layers of gauze, adding 2.5-3 parts by weight of auxiliary materials and 0.025-0.03 part by volume of defoaming agent into the filtrate to serve as culture materials;
(2) injecting the culture material with 350-370 volume parts into a culture tank, sterilizing at 124 ℃ for 35 minutes, and cooling to below 30 ℃; inoculating 0.8-1.2 parts by volume of first-stage liquid strain into a culture tank under aseptic condition; culturing for 72-96 hr at 25 deg.C and air flow rate of more than 1.2 m/hr; obtaining a secondary liquid strain after the culture is finished, namely the composite liquid probiotic;
D. adding glucose oxidase: c, adding 5-50 g/kg of glucose oxidase into the composite bacterial liquid obtained in the step C to obtain a finished product;
the unit of the weight part is g or kg, and the corresponding unit of the volume part is mL or L.
As a preferred technical scheme of the invention, in the step A, the number of the live bacteria of the clostridium butyricum after activation and propagation is not less than 50 hundred million UFC/mL.
In a preferred embodiment of the present invention, in step B- (1), the culture solution comprises the following components in parts by weight: 10 parts of potato, 1.5 parts of brown sugar, 1 part of glucose, 3 parts of wheat bran, 2 parts of peptone, 2 parts of monopotassium phosphate, 1 part of magnesium sulfate, 1 part of agar and 75-80 parts of clear water.
As a preferred technical scheme of the invention, in the step B- (1), the content of the vitamin B1 is 4 mg/L.
As a preferred technical scheme of the invention, in the step B- (2), after the strains are uniformly mixed, shaking table shaking culture is carried out, the shaking frequency is 180 r/min, and the temperature of the shaking table is controlled at 24-26 ℃.
As a preferred technical scheme of the invention, in the step C- (1), the auxiliary materials comprise the following components in parts by weight: 1.35 parts of brown sugar, 0.9 part of glucose, 0.18 part of peptone, 0.18 part of monopotassium phosphate and 0.09 part of magnesium sulfate.
As a preferred technical scheme of the invention, in the step C- (2), the dynamic tank pressure is controlled to be between 0.02 and 0.04MPa in the culture process.
As a preferable technical scheme of the invention, in the step D, the addition amount of the glucose oxidase is 10-40 g/kg.
In a preferred embodiment of the present invention, in the step D, the amount of glucose oxidase added is 25 g/kg.
Adopt the produced beneficial effect of above-mentioned technical scheme to lie in:
based on the current situation of production research of the applicant unit, the invention is combined with local colleges and universities to research and develop a compound biological probiotic + glucose oxidase compound preparation with a brand-new dosage form, and the obtained new product has good practical value and brings a brand-new direction for the development of local industries.
The innovative new product developed by the invention at least has the following advantages:
the glucose can be converted as a feed additive, residual oxygen is removed, the original quality and taste of the feed are maintained, and the fresh-keeping and preservation time of the feed is obviously prolonged.
Secondly, a long-term (2-3 months) feeding test shows that the compound scheme of the composite biological probiotics and the glucose oxidase can regulate the balance of gastrointestinal flora, promote the digestion and absorption of feed and increase the appetite.
The compounding of the composite biological probiotics and the glucose oxidase can enhance the immunity of the organism to a certain extent and reduce the occurrence of diseases by more than 50 percent compared with the conventional feeding comprehensive disease through the dual functions of the probiotics and the enzyme preparation.
And fourthly, from the aspect of input-output ratio, the economic benefit of the breeding industry can be remarkably improved.
The composition is used as a new formulation and a new product which are developed and researched together with colleges and universities, has good practical effect and market value, and can drive the development of local industries.
Detailed Description
The following examples illustrate the invention in detail. The raw materials and various devices used in the invention are conventional commercially available products, and can be directly obtained by market purchase. Wherein, the Trichoderma reesei is purchased from North Nawa Biotechnology GmbH, Suzhou; the stock solution of Clostridium butyricum is available from Beijing Baifeng Tianxia company or Shanghai such as Jinjun liquor company, or Japanese imported stock solution can be adopted; glucose oxidase was purchased from Beijing Baiolai Pacobi technologies, Inc. The following parts by weight are in g or kg, corresponding parts by volume are in mL or L.
Example 1 preparation of composite flora raw material.
(1) And (3) taking clostridium butyricum stock solution to fully propagate and activate in warm solution with sufficient carbon source, wherein the number of viable bacteria after activation and propagation is not less than 50 hundred million UFC/mL.
(2) Meanwhile, mother culture of trichoderma reesei is carried out, trichoderma reesei blocks are inoculated to a PDA culture medium, and dark culture is carried out at 25 ℃ until hyphae grow over an inclined plane for later use.
Example 2, first-order liquid seed culture was prepared.
(1) Preparing a culture solution: the culture solution comprises the following components in parts by weight: 10 parts of potato, 1.5 parts of brown sugar, 1 part of glucose, 3 parts of wheat bran, 2 parts of peptone, 2 parts of monopotassium phosphate, 1 part of magnesium sulfate, 1 part of agar, 75-80 parts of clear water and natural pH value; and adding vitamin B1 into the culture solution, wherein the content of vitamin B1 is 4 mg/L.
(2) Placing the prepared culture solution into a triangular flask, sealing, sterilizing, cooling to below 25 deg.C, inoculating the mycelia of the mother strain obtained in step A, and inoculating 1-2 mycelia of the mother strain with length and width of 2mm into each 100ml of the culture solution; simultaneously adding 10-50ml of clostridium butyricum activation propagation liquid obtained in the step A; mixing the strains, shaking at 180 rpm in a shaking table at 24-26 deg.C for 2 days to obtain first-stage liquid strain.
Example 3 preparation of second-stage liquid seed culture.
(1) Adding 90-100 parts by weight of water into 9-11 parts by weight of potatoes, boiling until the potatoes are crisp but not rotten, adding 3.4-3.8 parts by weight of large wheat bran, filtering by using 6-8 layers of gauze, adding 2.5-3 parts by weight of auxiliary materials and 0.025-0.03 part by volume of defoaming agent into the filtrate to serve as culture materials; wherein, the auxiliary materials comprise the following components in parts by weight: 1.35 parts of brown sugar, 0.9 part of glucose, 0.18 part of peptone, 0.18 part of monopotassium phosphate and 0.09 part of magnesium sulfate.
(2) Injecting the culture material with 350-370 volume parts into a culture tank, sterilizing at 124 ℃ for 35 minutes, and cooling to below 30 ℃; inoculating 0.8-1.2 parts by volume of first-stage liquid strain into a culture tank under aseptic condition; culturing for 72-96 hr at 25 deg.C and air flow rate of more than 1.2 m/hr, and controlling dynamic tank pressure at 0.02-0.04MPa during culturing process; and obtaining a secondary liquid strain after the culture is finished, namely the composite liquid probiotic.
Example 4, compounding glucose oxidase.
Adding 10-30 g/kg of glucose oxidase into the composite bacterial liquid obtained in the embodiment 3 to obtain a new product of the biological probiotics + glucose oxidase composite preparation.
Example 5 use of a new product of a complex formulation of biological probiotics + glucose oxidase.
The feed additive is generally used as a feed additive, and the addition amount of the feed additive is about 5% w/w of the weight of the feed. The glucose can be converted to remove residual oxygen, and the fresh-keeping and preservation time of the feed is prolonged; the compound scheme of the composite biological probiotics and the glucose oxidase can regulate the balance of gastrointestinal flora, promote the digestion and absorption of feed, increase appetite, enhance the immunity of the organism to a certain extent and reduce the occurrence of diseases through the dual functions of the probiotics and the enzyme preparation.
The above description is only presented as an enabling solution for the present invention and should not be taken as a sole limitation on the solution itself.
Claims (9)
1. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed is characterized by comprising the following steps: the method comprises the following preparation steps:
A. preparing a composite flora raw material:
(1) taking clostridium butyricum stock solution to fully propagate and activate in warm solution with sufficient carbon source for standby;
(2) simultaneously, carrying out mother culture of trichoderma reesei, inoculating trichoderma reesei blocks to a PDA culture medium, and carrying out dark culture at 25 ℃ until hyphae grow over an inclined plane for later use;
B. preparing a first-stage liquid strain:
(1) preparing a culture solution; the culture solution comprises the following components in parts by weight: 10-15 parts of potato, 1-1.5 parts of brown sugar, 1-1.5 parts of glucose, 3-4 parts of wheat bran, 1-2 parts of peptone, 1-2 parts of monopotassium phosphate, 1-1.5 parts of magnesium sulfate, 1 part of agar and 75-80 parts of clear water, wherein the pH value is natural; adding vitamin B1 into the culture solution;
(2) placing the prepared culture solution into a triangular flask, sealing, sterilizing, cooling to below 25 deg.C, inoculating the mycelia of the mother strain obtained in step A, and inoculating 1-2 mycelia of the mother strain with length and width of 2mm into each 100ml of the culture solution; simultaneously adding 10-50ml of clostridium butyricum activation propagation liquid obtained in the step A; mixing the strains, and culturing for 1-2.5 days to obtain first-stage liquid strain;
C. preparing a secondary liquid strain:
(1) adding 90-100 parts by weight of water into 9-11 parts by weight of potatoes, boiling until the potatoes are crisp but not rotten, adding 3.4-3.8 parts by weight of large wheat bran, filtering by using 6-8 layers of gauze, adding 2.5-3 parts by weight of auxiliary materials and 0.025-0.03 part by volume of defoaming agent into the filtrate to serve as culture materials;
(2) injecting the culture material with 350-370 volume parts into a culture tank, sterilizing at 124 ℃ for 35 minutes, and cooling to below 30 ℃; inoculating 0.8-1.2 parts by volume of first-stage liquid strain into a culture tank under aseptic condition; culturing for 72-96 hr at 25 deg.C and air flow rate of more than 1.2 m/hr; obtaining a secondary liquid strain after the culture is finished, namely the composite liquid probiotic;
D. adding glucose oxidase: c, adding 5-50 g/kg of glucose oxidase into the composite bacterial liquid obtained in the step C to obtain a finished product;
the unit of the weight part is g or kg, and the corresponding unit of the volume part is mL or L.
2. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed according to claim 1, characterized in that: in the step A, the number of the live bacteria of the clostridium butyricum after activation and propagation is not less than 50 hundred million UFC/mL.
3. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed according to claim 1, characterized in that: in the step B- (1), the culture solution comprises the following components in parts by weight: 10 parts of potato, 1.5 parts of brown sugar, 1 part of glucose, 3 parts of wheat bran, 2 parts of peptone, 2 parts of monopotassium phosphate, 1 part of magnesium sulfate, 1 part of agar and 75-80 parts of clear water.
4. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed according to claim 1, characterized in that: in the step B- (1), the content of the vitamin B1 is 4 mg/L.
5. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed according to claim 1, characterized in that: in the step B- (2), after the strains are uniformly mixed, shaking table shaking culture is carried out, the shaking frequency is 180 r/min, and the temperature of the shaking table is controlled at 24-26 ℃.
6. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed according to claim 1, characterized in that: in the step C- (1), the auxiliary materials comprise the following components in parts by weight: 1.35 parts of brown sugar, 0.9 part of glucose, 0.18 part of peptone, 0.18 part of monopotassium phosphate and 0.09 part of magnesium sulfate.
7. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed according to claim 1, characterized in that: in the step C- (2), the dynamic tank pressure is controlled to be between 0.02 and 0.04MPa in the culture process.
8. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed according to claim 1, characterized in that: in the step D, the addition amount of the glucose oxidase is 10-40 g/kg.
9. The preparation method of the glucose oxidase probiotic additive for poultry livestock feed according to claim 1, characterized in that: in the step D, the addition amount of the glucose oxidase is 25 g/kg.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114668079A (en) * | 2022-03-14 | 2022-06-28 | 临汾市八方通达饲料股份有限公司 | Propagating and healthy core material, biological feed and preparation method thereof |
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Application publication date: 20201023 |