CN111758544A - Water culture seedling hardening method and device for beautiful millettia root tissue culture seedlings - Google Patents
Water culture seedling hardening method and device for beautiful millettia root tissue culture seedlings Download PDFInfo
- Publication number
- CN111758544A CN111758544A CN202010615250.1A CN202010615250A CN111758544A CN 111758544 A CN111758544 A CN 111758544A CN 202010615250 A CN202010615250 A CN 202010615250A CN 111758544 A CN111758544 A CN 111758544A
- Authority
- CN
- China
- Prior art keywords
- nutrient solution
- water culture
- seedlings
- beautiful millettia
- tank body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 134
- 241000521581 Millettia Species 0.000 title claims abstract description 85
- 238000000034 method Methods 0.000 title claims abstract description 25
- 235000015097 nutrients Nutrition 0.000 claims abstract description 157
- 238000005286 illumination Methods 0.000 claims abstract description 46
- 239000002773 nucleotide Substances 0.000 claims abstract description 11
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 11
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims abstract description 10
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 41
- 238000010438 heat treatment Methods 0.000 claims description 25
- 241000196324 Embryophyta Species 0.000 claims description 16
- 239000013589 supplement Substances 0.000 claims description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 238000005303 weighing Methods 0.000 claims description 11
- REZQBEBOWJAQKS-UHFFFAOYSA-N triacontan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO REZQBEBOWJAQKS-UHFFFAOYSA-N 0.000 claims description 10
- 210000005056 cell body Anatomy 0.000 claims description 8
- 241001098027 Callerya speciosa Species 0.000 claims description 7
- 230000005489 elastic deformation Effects 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 6
- 229910019934 (NH4)2MoO4 Inorganic materials 0.000 claims description 5
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims description 5
- 239000005985 Paclobutrazol Substances 0.000 claims description 5
- -1 compound sodium nitrophenolate Chemical class 0.000 claims description 5
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims description 5
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 claims description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 4
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 4
- 239000006013 carbendazim Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000000249 desinfective effect Effects 0.000 claims description 4
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 229910017604 nitric acid Inorganic materials 0.000 claims description 4
- 206010010904 Convulsion Diseases 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 230000036461 convulsion Effects 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- CDUFCUKTJFSWPL-UHFFFAOYSA-L manganese(II) sulfate tetrahydrate Chemical compound O.O.O.O.[Mn+2].[O-]S([O-])(=O)=O CDUFCUKTJFSWPL-UHFFFAOYSA-L 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 241000220317 Rosa Species 0.000 claims description 2
- 230000012010 growth Effects 0.000 abstract description 13
- 230000004083 survival effect Effects 0.000 abstract description 12
- 230000009286 beneficial effect Effects 0.000 abstract description 6
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 210000001519 tissue Anatomy 0.000 description 38
- 230000001276 controlling effect Effects 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 7
- 235000016709 nutrition Nutrition 0.000 description 7
- 230000035764 nutrition Effects 0.000 description 7
- 238000002791 soaking Methods 0.000 description 6
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 5
- 238000005728 strengthening Methods 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 206010006458 Bronchitis chronic Diseases 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 244000166124 Eucalyptus globulus Species 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 206010050031 Muscle strain Diseases 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 208000007451 chronic bronchitis Diseases 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002686 phosphate fertilizer Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 201000003068 rheumatic fever Diseases 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Hydroponics (AREA)
Abstract
The invention discloses a water culture hardening method of beautiful millettia root tissue culture seedlings, which comprises the following steps: 1) preparation before transplanting: sterilizing a seedling hardening environment and preparing nutrient solution with the pH value of 5.8-6.5, wherein each 10L of the nutrient solution contains 0.01-0.05mg of brassinolide, 5-10mg of nucleotide and other nutrient components; 2) planting water culture seedlings: planting the disinfected beautiful millettia root seedlings on a planting plate, placing the planting plate on a cultivation groove filled with nutrient solution, and immersing one part of a root system in the nutrient solution while the other part is not immersed; 3) water culture management: controlling the temperature, humidity and illumination intensity of the seedling exercising environment according to the regulations, replacing the nutrient solution when the root system is immersed in the nutrient solution not more than 1/3, and exercising the seedlings for a period of time. The invention utilizes the promoting effect of brassinolide and nucleotide, ensures that the moisture and the nutrient of the root system are sufficient, and keeps part of the root to be contacted and breathed with air, thereby being beneficial to the growth of the root system and improving the transplanting survival rate.
Description
Technical Field
The invention relates to the technical field of hardening tissue culture seedlings. More specifically, the invention relates to a water culture seedling hardening method and a seedling hardening device for beautiful millettia root tissue culture seedlings.
Background
The tissue culture seedling is acclimatized, and the aims of improving the adaptability of the tissue culture seedling to external environmental conditions, improving the photosynthesis capability of the tissue culture seedling, promoting the robustness of the tissue culture seedling and finally achieving the aim of improving the transplanting survival rate of the tissue culture seedling are fulfilled. The temperature, humidity, illumination and sterile environment factors are generally required to be adjusted for domestication, and the domestication is similar to the environmental conditions during culture within a few days; in the later period of acclimatization, the conditions are similar to the expected cultivation conditions, so that the aim of gradual adaptation is fulfilled. According to different species, the domestication of the tissue culture seedlings generally needs 3-5 weeks. Some species may not need special hardening off, or hardening off and transplanting are synchronized. For example, after the test-tube plantlet of eucalyptus with stronger vitality ratio is directly transplanted, the transplantation can be ensured to survive successfully by shading, preventing diseases, strengthening water spraying and the like. However, tissue culture seedlings of some species (beautiful millettia root seedlings researched by the invention) need to be hardened to ensure the survival rate of transplantation.
Millettia speciosa (Millettia speciosa champ) is Millettia speciosa (Millettia speciosa champ) belonging to Millettia of Papilionaceae of Leguminosae. The beautiful millettia root is used as a medicine, has mild nature and sweet taste, enters kidney and lung channels, and has the effects of activating collaterals, strengthening tendons, moistening lung, tonifying deficiency, strengthening spleen and strengthening body. Clinical studies have shown that: the beautiful millettia root has obvious curative effect on pulmonary tuberculosis, cough due to lung deficiency, weakness after illness, lumbar muscle strain, leukorrhagia due to kidney deficiency, rheumatic arthritis, chronic bronchitis and other chronic diseases. Since the 70 s of the 20 th century, the masses in the south of Ling began to use beautiful millettia root to prepare medicated diet, medicated wine or soup, and the like, and the beautiful millettia root is widely used as a main raw material for the production of Chinese patent medicines such as strong body-building capsules, waist-strengthening and kidney-invigorating pills, and the like. In recent years, along with the enhancement of health care consciousness and the improvement of living standard of people, the market demand of the beautiful millettia root is rapidly increased, and the planting prospect is wide.
At present, the seedling breeding of beautiful millettia root is mainly based on tissue culture, so the transplanting survival of tissue culture seedlings is very important, the tissue culture seedlings are generally transplanted into greenhouse nutrient cups for hardening seedlings, but the greenhouse nutrient cup hardening seedlings of the tissue culture seedlings are not well controlled in fertilization, the water and fertilizer configuration is not good, the root systems of the seedlings hardened in the greenhouse grow slowly and the transplanting is not facilitated. The test tube seedling leaf tissue has large gaps, water is easy to lose, and reasonable water supply is also important. If the water supply is insufficient, wilting is easily caused, the growth of plants is influenced, and the transplanting is not facilitated. Therefore, the greenhouse nutrition cup seedling hardening can ensure that the moisture and the nutrients of the root system are sufficient only by frequently and reasonably watering and fertilizing, but frequent watering wastes water sources and nutrient substances, and the nutrient substances are easy to lose, so that the greenhouse nutrition cup seedling hardening is not beneficial to popularization and application.
Therefore, in order to solve the technical problems, the invention provides a water culture seedling hardening method and a water culture seedling hardening device for beautiful millettia root tissue culture seedlings. Due to different characteristics of the tissue culture seedlings, attention needs to be paid to the configuration of a nutrient solution and a water culture control method for water culture of the tissue culture seedlings, and the problem that the roots of the tissue culture seedlings are damaged due to unreasonable configuration of the nutrient solution and the growth of the beautiful millettia root seedlings is influenced is avoided.
Disclosure of Invention
The invention provides a water culture hardening method of beautiful millettia root system, aiming at the problems that the root system of the hardened seedlings in a greenhouse nutrition cup in the prior art grows slowly and the transplanting survival rate is influenced. The nutrient solution in the cultivation groove is absorbed and utilized to the maximum extent and then replaced, the utilization rate of the nutrient solution can be improved, waste is avoided, the nutrient solution can be replaced under the condition of the maximum utilization rate, and bacteria breeding of the nutrient solution in the cultivation groove is reduced. The root system of the beautiful millettia root seedlings after hardening is developed, the stems are thick and strong, the leaf color is dark green, the growth vigor can be quickly recovered when the beautiful millettia root seedlings are transferred to a field for planting, and the transplanting survival rate is improved.
The invention provides a water culture seedling exercising device aiming at the problem that root systems of beautiful millettia root-growing seedlings of which the specified length needs to be controlled are immersed in nutrient solution to ensure the sufficiency of the moisture and the nutrient of the root systems and are beneficial to replacement after the nutrient solution in a tank body is absorbed to the maximum extent.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for hydroponic acclimatization of beautiful millettia root tissue culture seedlings, comprising the steps of:
step one, preparation before transplanting: cleaning and disinfecting a water culture box and a culture groove for containing nutrient solution, and filling the nutrient solution into the culture groove, wherein each 10L of the nutrient solution contains the following components: ca (NO)3)2.4H2O 720-750mg、KNO3430-480mg、KH2PO4110-130mg、MgSO4.7H2O 380-410mg、Na2Fe-EDTA 21-23mg、H3BO31.5-1.9mg、CuSO4.5H2O 0.3-0.6mg、MnSO4.4H2O 0.2-0.5mg、ZnSO4.7H2O 0.4-0.8mg、(NH4)2MoO40.1-0.3mg, paclobutrazol 300-400mg, triacontanol 0.5-1mg, compound sodium nitrophenolate 8-14mg, brassinolide 0.01-0.05mg and nucleotide 5-10 mg;
step two, field planting of water culture seedlings: fixedly planting the disinfected beautiful millettia root seedlings into the cultivation holes of the planting plates, and placing the planting plates on the cultivation grooves filled with the nutrient solution to ensure that one part of the root systems at the bottoms of the beautiful millettia root seedlings is immersed in the nutrient solution and the other part of the root systems is not immersed in the nutrient solution;
step three, water culture management: after the rooting seedlings of beautiful millettia root are fixedly planted, the pH value of the nutrient solution is kept to be 5.8-6.5, and when the root system of the beautiful millettia root immersed in the nutrient solution is not more than 1/3, the nutrient solution is replaced; in the early stage and the middle stage of seedling exercising, the temperature in the daytime is 24-28 ℃, the temperature at night is 15-18 ℃, the relative humidity is 60-80%, the illumination intensity is 2000-; the temperature, humidity and illumination intensity in the later period of seedling hardening tend to be similar to those in the expected cultivation environment day by day.
Preferably, in the second step, the method for sterilizing the beautiful millettia root seedlings comprises the step of soaking the beautiful millettia root seedlings in 0.2 mass percent carbendazim solution for 5 to 10 minutes.
Preferably, the reagent for adjusting the pH is a 5% by mass nitric acid solution and a 5% by mass sodium hydroxide solution.
The invention also provides a water culture hardening device for the millettia speciosa champ tissue culture seedlings, which comprises a water culture box for hardening the seedlings, a culture groove for providing nutrient solution for the tissue culture seedlings of the water culture box, a field planting plate arranged above the culture groove and used for field planting of the tissue culture seedlings, a plurality of electric telescopic rods for assisting the groove body to ascend, and a temperature and humidity illumination system for controlling the temperature, humidity and illumination in the water culture box;
the cultivation tank comprises a tank body and a spring group base used for adjusting the distance between the bottom of the tank body and the planting plate according to the weight of nutrient solution in the tank body;
the spring group base comprises a bottom plate and a plurality of springs, the springs are the same in length, the springs are vertically and uniformly distributed on the bottom plate in parallel, one ends of the springs are fixedly connected with the bottom plate, and the other ends of the springs are fixedly connected with the bottom surface of the groove body;
the upper end of the tank body is open, a field planting plate is arranged on the upper opening of the tank body and is fixed with the water planting tank through a fixing piece, the distance between the bottom of the tank body and the field planting plate is changed through elastic deformation of a spring set base, when nutrient solution in the tank body increases, the weight of the tank body increases, the spring set base is compressed, and the distance between the bottom of the tank body and the field planting plate increases; when the nutrient solution in the tank body is reduced, the weight of the tank body is reduced, the spring group base gradually recovers to the original shape, and the distance between the bottom of the tank body and the planting plate is reduced; a drain outlet and a liquid inlet are arranged at the bottom of the tank body, and a drain electromagnetic valve and a liquid inlet electromagnetic valve are respectively arranged on the drain outlet and the liquid inlet;
a plurality of electric telescopic handle that supplementary cell body rose evenly distributed is in the below of cell body bottom, and electric telescopic handle's one end is fixed to be set up in the bottom of water planting case, and the other end can control the extension and support to press the cell body bottom and shrink and keep away from the cell body bottom.
Preferably, be provided with the gate that is used for the immigration and pulls out the field planting board on the lateral wall of water planting case, correspond on the gate and be provided with the door plant, can dismantle the intercommunication respectively on drain and the inlet and have blow off pipe and feed liquor pipe, blow off pipe and feed liquor pipe are the hose.
Preferably, the fixing piece is a right-angled S-shaped connecting plate, the upper end and the lower end of the fixing piece are horizontal plates, the middle of the fixing piece is a vertical plate, and two ends of the vertical plate are respectively and vertically and fixedly connected with one end of the upper horizontal plate and one end of the lower horizontal plate; the other ends of the upper horizontal plates of the fixing pieces are fixedly connected with the side wall of the water culture box, the other ends of the lower horizontal plates of the fixing pieces extend towards the inside of the water culture box, and the extending planes of the lower horizontal plates of the fixing pieces are used for placing the planting plates; spaces formed among the upper horizontal plates, the vertical plates and the side walls of the water culture boxes of the plurality of fixing pieces are used for reserving spaces for the up-and-down movement of the walls of the tank body; and a vertical guide rail is arranged on the side wall of the water culture box and is used for enabling the wall of the tank body to slide up and down along the side wall of the water culture box.
Preferably, the elastic coefficients of the plurality of springs are the same, a weighing sensor is fixed on the bottom surface in the hydroponic tank, and a bottom plate of the spring group base is pressed on the weighing sensor and used for detecting the change condition of the weight of the nutrient solution in the tank body; the pollution discharge electromagnetic valve, the liquid inlet electromagnetic valve, the weighing sensor and the controller of the temperature and humidity illumination system are electrically connected.
Preferably, the temperature and humidity illumination system comprises a shading device, an illumination intensity sensor, a temperature sensor, a humidity sensor, an exhaust fan, a heating air pipe, a plant light supplement lamp and a controller;
the shading device is arranged on the outer wall of the water culture box and is used for shading the irradiation of natural light to the water culture box according to the illumination intensity requirement of the water culture box;
the plant light supplement lamp is arranged in the water culture box and used for controlling starting and adjusting the illumination intensity when the shading device cannot meet the illumination intensity requirement of the water culture box;
the illumination intensity sensor, the temperature sensor and the humidity sensor are arranged in the water culture box and used for detecting the illumination intensity, the temperature and the humidity in the water culture box and used as feedback signals for controlling the actions of the shading device, the plant light supplement lamp, the exhaust fan and the heating air pipe;
the water culture box is characterized in that the exhaust fan is arranged on the side wall of the water culture box and used for controlling the opening and closing and the rotating speed of the exhaust fan according to the temperature and humidity requirements of the water culture box;
the heating air pipe is arranged at the top of the water culture box and used for controlling the opening and closing of the heating air pipe according to the temperature requirement of the water culture box; the heating air pipe consists of a heating fan and a pipeline, and the outlet of the heating fan is communicated with the water culture box through the pipeline;
the shading device, the illumination intensity sensor, the temperature sensor, the humidity sensor, the exhaust fan, the plant light supplement lamp and the heating air pipe are respectively and electrically connected with the controller;
wherein, shade is collapsible puggaree, the water planting case wall is made by clear glass, is provided with a plurality of ventilation holes on the lateral wall of water planting case for natural draft and air exhauster convulsions adjust the temperature.
Preferably, the trough body also comprises a pH sensor and a liquid level sensor;
the pH sensor is arranged on the bottom side wall in the groove body;
the liquid level sensor is arranged below the planting plate and used for detecting the liquid level of the root system soaked in the nutrient solution;
the pH sensor and the liquid level sensor are respectively electrically connected with the controller, and the controller sends out corresponding control signals or alarm signals according to signals of the pH sensor and the liquid level sensor.
Preferably, the control principle that the root system of the beautiful millettia root seedling with the specified length is controlled by the water culture seedling exercising device to be immersed in the nutrient solution is as follows: in the early stage of hardening, the electric telescopic rod is controlled to extend step by step to push the groove body to rise so as to immerse a part of the root system at the bottom of the beautiful millettia root-growing seedling into the nutrient solution; in the middle and later seedling hardening periods, the electric telescopic rod is shortened, the distance between the bottom of the groove body filled with nutrient solution and the planting plate is changed through the elastic deformation of the spring group base, when the nutrient solution in the groove body increases, the weight of the groove body is increased, the spring group base is compressed, and the distance between the bottom of the groove body and the planting plate is increased; when the nutrient solution in the groove body is reduced, the weight of the groove body is reduced, the base of the spring group gradually recovers to the original shape, and the distance between the bottom of the groove body and the planting plate is reduced.
The invention at least comprises the following beneficial effects:
1. the nutrient solution prepared by the invention is prepared according to the ratio of nutrient components and moisture absorbed by the beautiful millettia root system, and is beneficial to the absorption of the nutrient components and the moisture by the beautiful millettia root system, so that the ratio of the nutrient components is basically unchanged after the nutrient solution in the cultivation tank is absorbed and reduced in the seedling hardening process, and the utilization rate is favorably improved. In addition, a part of the root is not immersed in the nutrient solution, and a part of the root is left to be contacted with the air for breathing, so that the problem that the root is rotten due to the generation of alcohol and the growth of the root system is influenced due to the fact that the root is over-breathed without oxygen is solved.
2. The nutrient solution prepared by the invention utilizes the brassinolide to promote the effective absorption of nucleotide in the root system and the stem leaves of the beautiful millettia root seedlings, regulates the growth and development of the seedlings, promotes the vigorous growth, the developed root system and the stout of the beautiful millettia root tissue culture seedlings, and is favorable for improving the transplanting survival rate. Although the indolebutyric acid also has the function of promoting the effective absorption of nucleotide in the root system and the stem and leaf of the beautiful millettia root stock, the indolebutyric acid in the water culture can inhibit the growth of the root system of the beautiful millettia root stock on the contrary when soaking the root system for a long time, so the invention avoids the adverse effect of the indolebutyric acid in the water culture, ensures that the brassinolide and the nucleotide play the greatest promoting role, promotes the vigorous growth, the developed root system and the stout of the beautiful millettia root stock tissue culture seedling, and is favorable for improving the transplanting survival rate.
3. In the early stage of hardening seedlings, the electric telescopic rods are controlled to extend step by step to push the groove body to rise so as to immerse a part of the root system at the bottom of the root system of the beautiful millettia root seedlings into the nutrient solution; in the middle and later seedling hardening periods, the electric telescopic rod is shortened, the distance between the bottom of the groove body filled with nutrient solution and the planting plate is changed through the elastic deformation of the spring group base, when the nutrient solution in the groove body is increased, the weight of the groove body is increased, the spring group base is compressed, the distance between the bottom of the groove body and the planting plate is increased, and the root system cannot be soaked beyond the position; when the nutrient solution in the groove body is reduced, the weight of the groove body is reduced, the base of the spring group gradually recovers to the original shape, the distance between the bottom of the groove body and the planting plate is reduced, and the situation that the root system cannot be soaked or the soaking position is too low is avoided. Therefore, after the tissue culture seedlings continuously absorb the nutrient solution, the nutrient solution can be continuously reduced, but the proportion of the soaked tissue culture seedlings in the root system is basically unchanged, the sufficiency of the moisture and the nutrients of the root system is ensured, and the replacement of the root system in the groove body after the nutrient solution is absorbed to the maximum degree is facilitated. The relationship between the amount of the nutrient solution and the distance between the bottom of the groove body and the planting plate is analyzed as follows: setting the number of the springs as n, the elastic coefficient of each spring as k, setting the total weight of the nutrient solution in the groove body and the groove body as F, obtaining the proportional relation between the change of the weight of the nutrient solution in the groove body and the expansion amount delta X of the base of the spring group according to the formula F as nk delta X, and under the condition that the volume of the groove body is fixed, the weight of the nutrient solution in the groove body is proportional to the liquid level height of the nutrient solution in the groove body. Therefore, the nutrient solution in the cultivation tank is absorbed and utilized to the maximum extent and then replaced, so that the utilization rate of the nutrient solution can be improved, waste is avoided, the nutrient solution can be replaced under the condition of the maximum utilization rate, and bacteria breeding of the nutrient solution in the cultivation tank is reduced. The sufficiency of moisture and nutrients of the root system is ensured, a part of the root is kept to be contacted with air for breathing, so that the root system of the beautiful millettia root seedling after hardening is developed, the stem is thick and strong, the leaf color is dark green, the growth vigor can be quickly recovered when the beautiful millettia root seedling is transferred to a field for planting, and the transplanting survival rate is improved.
4. According to the invention, the bottom plate of the spring group base is pressed on the weighing sensor, so that the amount of the nutrient solution added into the tank body each time can be controlled by setting the range of the weight of the nutrient solution in the tank body and the total weight of the tank body, the amount of the residual nutrient solution in the tank body at present can be detected, and the liquid changing operation can be executed when the amount of the residual nutrient solution in the tank body is regulated.
5. According to the invention, the liquid level sensor is arranged below the field planting plate, so that the nutrient solution reaching the position of the liquid level sensor is the root system soaking position of the beautiful millettia root seedling meeting the specified length, and when the nutrient solution is lower than the position of the liquid level sensor, a corresponding control signal or alarm signal is sent out, so that a worker can conveniently check or replace the nutrient solution.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a schematic structural diagram of an implementation form of a water culture seedling exercising device for beautiful millettia root tissue culture seedlings of the invention;
wherein, the water culture box 10; a plant light supplement lamp 11; an exhaust fan 12; a fixing member 13; a planting plate 14; a guide rail 15; a liquid level sensor 16; a vent hole 17; a heating fan 18; a blow-off pipe 19; a blowdown electromagnetic valve 20; a drain outlet 21; a tank body 22; a liquid inlet 23; a liquid inlet electromagnetic valve 24; a liquid inlet pipe 25; a spring 26; the electric telescopic rod 27.
Detailed Description
The present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description text.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
< example 1>
A water culture hardening method for beautiful millettia root tissue culture seedlings, which uses the water culture hardening device shown in the attached figure 1, comprises the following steps:
step one, preparation before transplanting: cleaning and disinfecting a water culture box and a culture groove for containing nutrient solution, and filling the nutrient solution into the culture groove, wherein each 10L of the nutrient solution contains the following components: ca (NO)3)2.4H2O 720mg、KNO3430mg、KH2PO4110mg、MgSO4.7H2O 380mg、Na2Fe-EDTA 21mg、H3BO31.5mg、CuSO4.5H2O 0.3mg、MnSO4.4H2O0.2mg、ZnSO4.7H2O 0.4mg、(NH4)2MoO40.1mg, paclobutrazol 300mg, triacontanol 0.5mg, compound sodium nitrophenolate 8mg, brassinolide 0.01mg and nucleotide 5 mg; wherein the pH value of the nutrient solution is5.8-6.5, wherein the reagents for adjusting the pH are a nitric acid solution with the mass fraction of 5% and a sodium hydroxide solution with the mass fraction of 5%
Step two, field planting of water culture seedlings: soaking the beautiful millettia root-growing seedlings in 0.2 mass percent carbendazim solution for 5 minutes, then transplanting the disinfected beautiful millettia root-growing seedlings into the cultivation holes of a planting plate, and placing the planting plate on a cultivation groove filled with the nutrient solution, so that one part of the root system at the bottom of the beautiful millettia root-growing seedlings is immersed in the nutrient solution, and the other part is not immersed in the nutrient solution;
step three, water culture management: after the rooting seedlings of beautiful millettia root are fixedly planted, the pH value of the nutrient solution is kept to be 5.8-6.5, and when the root system of the beautiful millettia root immersed in the nutrient solution is not more than 1/3, the nutrient solution is replaced; in the early stage and the middle stage of seedling exercising, the temperature in the daytime is 24-28 ℃, the temperature at night is 15-18 ℃, the relative humidity is 60-80%, the illumination intensity is 2000-; the temperature, humidity and illumination intensity in the later stage of seedling exercising change day by day and tend to be 18-20 ℃ in the daytime, 15-17 ℃ in the nighttime, 50-60% for humidity and 1800 plus 2000lux for illumination intensity of the expected cultivation environment in the expected cultivation environment.
< example 2>
A water culture hardening method for beautiful millettia root tissue culture seedlings, which uses the water culture hardening device shown in the attached figure 1, comprises the following steps:
step one, preparation before transplanting: cleaning and disinfecting a water culture box and a culture groove for containing nutrient solution, and filling the nutrient solution into the culture groove, wherein each 10L of the nutrient solution contains the following components: ca (NO)3)2.4H2O 750mg、KNO3480mg、KH2PO4130mg、MgSO4.7H2O 410mg、Na2Fe-EDTA 23mg、H3BO31.9mg、CuSO4.5H2O 0.6mg、MnSO4.4H2O0.5mg、ZnSO4.7H2O 0.8mg、(NH4)2MoO40.3mg, paclobutrazol 400mg, triacontanol 1mg, compound sodium nitrophenolate 14mg, brassinolide 0.05mg and nucleotide 10 mg; wherein the pH value of the nutrient solution is 5.8-6.5, and the pH value is adjustedThe agent is a nitric acid solution with the mass fraction of 5 percent and a sodium hydroxide solution with the mass fraction of 5 percent
Step two, field planting of water culture seedlings: soaking the beautiful millettia root-growing seedlings in 0.2 mass percent carbendazim solution for 5 minutes, then transplanting the disinfected beautiful millettia root-growing seedlings into the cultivation holes of a planting plate, and placing the planting plate on a cultivation groove filled with the nutrient solution, so that one part of the root system at the bottom of the beautiful millettia root-growing seedlings is immersed in the nutrient solution, and the other part is not immersed in the nutrient solution;
step three, water culture management: after the rooting seedlings of beautiful millettia root are fixedly planted, the pH value of the nutrient solution is kept to be 5.8-6.5, and when the root system of the beautiful millettia root immersed in the nutrient solution is not more than 1/3, the nutrient solution is replaced; in the early stage and the middle stage of seedling exercising, the temperature in the daytime is 24-28 ℃, the temperature at night is 15-18 ℃, the relative humidity is 60-80%, the illumination intensity is 2000-; the temperature, humidity and illumination intensity in the later stage of seedling exercising change day by day and tend to be 18-20 ℃ in the daytime, 15-17 ℃ in the nighttime, 50-60% for humidity and 1800 plus 2000lux for illumination intensity of the expected cultivation environment in the expected cultivation environment.
< comparative example 1>
A method for hardening tissue culture seedlings of beautiful millettia root by water culture uses a conventional water culture box, when nutrient solution is reduced to submerge root systems not more than 1/3, the nutrient solution is not replaced, the nutrient solution is automatically added, so that 2/3-4/5 of the root systems of beautiful millettia root seedlings are immersed in the nutrient solution, and other steps are the same as those in embodiment 1.
< comparative example 2>
The invention relates to a water culture hardening method of beautiful millettia root tissue culture seedlings, which uses a water culture hardening device shown in figure 1, wherein the nutrient solution used in the method is that every 10L of the nutrient solution contains 4mg of indolebutyric acid, and other steps and components of the nutrient solution are the same as those in the embodiment 1.
< comparative example 3>
The invention discloses a water culture hardening method of beautiful millettia root tissue culture seedlings, which uses a water culture hardening device shown in figure 1, the nutrient solution used in the method is different from that in the embodiment 1, and other steps and components of the nutrient solution are the same as those in the embodiment 1. The method uses 10L nutrient solutionThe nutrient solution contains the following components: ca (NO)3)2.4H2O 760mg、KNO3520mg、NK4H2PO4540mg、MgSO4.7H2O820mg、Na2Fe-EDTA 75-98mg、H3BO35.6mg、CuSO4.5H2O 0.35mg、MnSO4.4H2O 0.26mg、ZnSO4.7H2O 0.45mg、(NH4)2MoO40.12mg, paclobutrazol 300mg, triacontanol 0.5mg, compound sodium nitrophenolate 8mg, brassinolide 0.01mg, nucleotide 2mg and indolebutyric acid 4 mg.
< comparative example 4>
A greenhouse nutrition cup hardening method for beautiful millettia root tissue culture seedlings is characterized in that conventional greenhouse nutrition cups are used for hardening seedlings, the nutrient solution is manually poured in the cups at intervals, the nutrient solution is poured at intervals from 8 am to 6 pm in the early stage of hardening seedlings, 10s is sprayed at intervals of 10min, 6 pm to 8 pm in the morning every day, and 10s is sprayed at intervals of 20 min; spraying 20s every 8 am to 6 am in the middle and later periods of hardening off, spraying 20s every 6 am to 8 am every 40min, wherein the nutrient solution components are the same as those in example 1, and the temperature, humidity and illumination intensity set for hardening off are the same as those in example 1. The greenhouse nutrition cup is characterized in that the substrate of the greenhouse nutrition cup is prepared by mixing soil and phosphate fertilizer in a mass ratio of 100: 1, composting to obtain a mixture, uniformly mixing the mixture and turf in a mass ratio of 1: 1, and then sterilizing.
< control 1>
Taking the cattle high-strength rooted seedlings before seedling hardening as the seedlings of the control 1.
< Effect test >
Adopting the seedling hardening method of the embodiment 1-2 and the comparative example 1-4, taking 30 beautiful millettia root seedlings for hardening for 18 days, and randomly taking 25 seedlings for transplanting after hardening. And a control 1 is to take 5 millettia speciosa champ rooted seedlings of the same batch which are not subjected to seedling hardening and transplant.
The transplanting environments of the examples 1-2 and the comparative examples 1-4 and the comparison 1 are the same, the transplanting substrates are the same, and the post-management is the same. The average plant height, average root length, average root number and survival rate of 30 days after transplanting were counted for the seedlings after hardening off in examples 1-2 and comparative examples 1-4 and the seedlings of control 1, as shown in table 1.
Table 1:
the data in table 1 show that, firstly, the brassinolide is utilized to promote the nucleotide to be effectively absorbed in the root system and stem leaves of the beautiful millettia root seedlings, so that the growth and development of the seedlings are regulated, the vigorous growth, the developed root system and the thick and strong rhizome of the beautiful millettia root tissue culture seedlings are promoted, and the transplanting survival rate is favorably improved. Secondly, the nutrient solution is prepared according to the ratio of nutrient components and moisture absorbed by the root system of beautiful millettia root, so that the nutrient components and the moisture are absorbed by the root system of beautiful millettia root, the nutrient components are absorbed and reduced in the process of hardening seedlings, the ratio of the nutrient components is basically unchanged, the contact liquid level of the root system and the nutrient solution is improved through the device, the sufficiency of the moisture and the nutrient of the root system is ensured, a part of the root is kept to be in contact with air for breathing, the growth of the root system is facilitated, and the utilization rate of the nutrient solution is improved. Thirdly, the nutrient solution in the cultivation tank is absorbed and utilized to the maximum extent and then replaced, so that the utilization rate of the nutrient solution can be improved, waste is avoided, the nutrient solution can be replaced under the condition of the maximum utilization rate, and the influence of bacteria breeding on the root system of the nutrient solution in the cultivation tank is avoided. The invention can develop the root system of the beautiful millettia root seedlings after hardening, is beneficial to quickly restoring the growth vigor when the beautiful millettia root seedlings are transferred to fields for planting, and improves the transplanting survival rate.
Fig. 1 shows an implementation form of a water culture hardening device for beautiful millettia root tissue culture seedlings, which comprises a water culture box 10 for hardening seedlings, a culture groove for providing nutrient solution for the tissue culture seedlings of the water culture box 10, a field planting plate 14 for field planting of the tissue culture seedlings arranged above the culture groove, a plurality of electric telescopic rods 27 for assisting the groove body to rise, and a temperature and humidity illumination system for controlling the temperature and humidity and illumination in the water culture box 10;
the cultivation tank comprises a tank body 22 and a spring group base used for adjusting the distance between the bottom of the tank body 22 and the planting plate 14 according to the weight of nutrient solution in the tank body 22;
the spring group base comprises a bottom plate and a plurality of springs 26, the plurality of springs 26 are the same in length, the plurality of springs 26 are vertically and uniformly distributed on the bottom plate in parallel, one end of each spring 26 is fixedly connected with the bottom plate, and the other end of each spring 26 is fixedly connected with the bottom surface of the groove body 22;
the upper end of the tank body 22 is open, a field planting plate 14 is arranged on the upper opening of the tank body 22, the field planting plate 14 is fixed with the water culture tank 10 through a fixing piece 13, the distance between the bottom of the tank body 22 and the field planting plate 14 is changed through elastic deformation of a spring set base, when nutrient solution in the tank body 22 increases, the weight of the tank body 22 increases, the spring set base is compressed, and the distance between the bottom of the tank body 22 and the field planting plate 14 increases; when the nutrient solution in the groove body 22 is reduced, the weight of the groove body 22 is reduced, the spring group base gradually restores to the original shape, and the distance between the bottom of the groove body 22 and the planting plate 14 is reduced; a sewage draining outlet 21 and a liquid inlet 23 are arranged at the bottom of the groove body 22, and a sewage draining electromagnetic valve 20 and a liquid inlet electromagnetic valve 24 are respectively arranged on the sewage draining outlet 21 and the liquid inlet 23;
the electric telescopic rods 27 for assisting the ascending of the groove bodies are uniformly distributed below the bottom of the groove bodies, one end of each electric telescopic rod 27 is fixedly arranged at the bottom of the water culture box 10, and the other end of each electric telescopic rod can control the extension to press the bottom of the groove body and contract to keep away from the bottom of the groove body.
The control principle of controlling the root system of the beautiful millettia root-growing seedlings with specified length to be immersed in the nutrient solution by the water planting seedling exercising device is as follows: in the early stage of hardening, the electric telescopic rod 27 is controlled to extend to gradually push the groove body to ascend so as to immerse a part of the root system at the bottom of the beautiful millettia root-growing seedling into the nutrient solution; in the middle and later seedling hardening periods, the electric telescopic rod is shortened, the distance between the bottom of the groove body 22 filled with nutrient solution and the planting plate 14 is changed through the elastic deformation of the spring group base, when the nutrient solution in the groove body 22 is increased, the weight of the groove body 22 is increased, the spring group base is compressed, and the distance between the bottom of the groove body 22 and the planting plate 14 is increased; when the nutrient solution in the groove body 22 is reduced, the weight of the groove body 22 is reduced, the spring set base gradually restores to the original shape, and the distance between the bottom of the groove body 22 and the planting plate 14 is reduced.
On the basis of the above embodiment, be provided with the gate that is used for immigration and pull out field planting board 14 on the lateral wall of water planting case 10, correspond on the gate and be provided with the door plant, the intercommunication can be dismantled respectively on drain 21 and the inlet 23 and have blow off pipe 19 and feed liquor pipe 25, blow off pipe 19 and feed liquor pipe 25 are the hose.
On the basis of the above embodiment, the fixing member 13 is a right-angled S-like connecting plate, the upper and lower ends of which are horizontal plates, and the middle of which is a vertical plate, and both ends of the vertical plate are respectively and vertically and fixedly connected with one end of the upper horizontal plate and one end of the lower horizontal plate; the other ends of the upper horizontal plates of the plurality of fixing pieces 13 are fixedly connected with the side wall of the water culture box 10, the other ends of the lower horizontal plates of the plurality of fixing pieces 13 extend towards the inside of the water culture box 10, and the extending planes of the lower horizontal plates of the plurality of fixing pieces 13 are used for placing the planting plates 14; spaces formed between the upper horizontal plates and the vertical plates of the plurality of fixing pieces 13 and the side walls of the hydroponic tank 10 are used for reserving a space for the up-and-down movement of the walls of the tank body 22; the side wall of the water culture box 10 is provided with a vertical guide rail 15 which is used for sliding the wall of the trough body 22 up and down along the side wall of the water culture box 10.
On the basis of the above embodiment, the elastic coefficients of the plurality of springs 26 are the same, a weighing sensor is fixed on the bottom surface in the hydroponic tank 10, and the bottom plate of the spring group base is pressed on the weighing sensor and used for detecting the change condition of the weight of the nutrient solution in the tank body 22; the pollution discharge electromagnetic valve 20, the liquid inlet electromagnetic valve 24, the weighing sensor and the controller of the temperature and humidity illumination system are electrically connected.
The invention arranges a weighing sensor between the spring group base and the bottom of the water culture box according to the following realization principle: the weight sensor detects the weight of the nutrient solution in the tank body and the total weight of the tank body in real time and transmits the weight of the nutrient solution and the total weight of the tank body to the controller as a feedback signal, and the controller sets the range of the weight of the nutrient solution in the tank body and the total weight of the tank body, can control the amount of the nutrient solution added into the tank body every time, detects the amount of the residual nutrient solution in the tank body at present, and can execute liquid changing operation when the amount of the residual nutrient solution in the tank body.
On the basis of the above embodiment, the temperature and humidity illumination system includes a light shielding device, an illumination intensity sensor, a temperature sensor, a humidity sensor, an exhaust fan 12, a heating air duct, a plant light supplement lamp 11 and a controller;
the shading device is arranged on the outer wall of the water culture box 10 and is used for shading the irradiation of natural light to the water culture box 10 according to the requirement of the illumination intensity of the water culture box 10;
the plant light supplement lamp 11 is arranged in the water culture box 10 and used for controlling starting and adjusting illumination intensity when the shading device cannot meet the illumination intensity requirement of the water culture box 10;
the illumination intensity sensor, the temperature sensor and the humidity sensor are arranged in the water culture box 10, and are used for detecting the illumination intensity, the temperature and the humidity in the water culture box 10 and used as feedback signals for controlling the actions of the shading device, the plant light supplement lamp 11, the exhaust fan 12 and the heating air pipe;
the exhaust fan 12 is arranged on the side wall of the water culture box 10 and used for controlling the opening and closing and the rotating speed of the exhaust fan 12 according to the temperature and humidity requirements of the water culture box 10;
the heating air pipe is arranged at the top of the water culture box 10 and used for controlling the opening and closing of the heating air pipe according to the temperature requirement of the water culture box 10; the heating air pipe consists of a heating fan 18 and a pipeline, and the outlet of the heating fan 18 is communicated with the water culture box 10 through the pipeline;
the shading device, the illumination intensity sensor, the temperature sensor, the humidity sensor, the exhaust fan 12, the plant light supplement lamp 11 and the heating air pipe are respectively and electrically connected with the controller;
wherein, shade is collapsible puggaree, water planting case 10 walls are made by transparent glass, are provided with a plurality of ventilation holes 17 on the lateral wall of water planting case 10 for natural draft and 12 convulsions of air exhauster adjust the temperature.
On the basis of the above embodiment, the tank body 22 further comprises a pH sensor and a liquid level sensor 16;
the pH sensor is arranged on the side wall of the bottom in the groove body 22;
the liquid level sensor 16 is arranged 2-3cm below the planting plate 14 and used for detecting the liquid level of the root system soaked in the nutrient solution;
the pH sensor and the liquid level sensor 16 are respectively electrically connected with the controller, and the controller sends out corresponding control signals or alarm signals according to signals of the pH sensor and the liquid level sensor 16.
While embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields of adaptation of the invention, and further modifications can be easily implemented by those skilled in the art, so that the invention is not limited to the specific details and the examples shown herein, without departing from the general concept defined by the claims and the scope of equivalents.
Claims (10)
1. The water culture hardening method of the beautiful millettia root tissue culture seedling is characterized by comprising the following steps:
step one, preparation before transplanting: cleaning and disinfecting a water culture box and a culture groove for containing nutrient solution, and filling the nutrient solution into the culture groove, wherein each 10L of the nutrient solution contains the following components: ca (NO)3)2.4H2O 720-750mg、KNO3430-480mg、KH2PO4110-130mg、MgSO4.7H2O 380-410mg、Na2Fe-EDTA 21-23mg、H3BO31.5-1.9mg、CuSO4.5H2O 0.3-0.6mg、MnSO4.4H2O 0.2-0.5mg、ZnSO4.7H2O 0.4-0.8mg、(NH4)2MoO40.1-0.3mg, paclobutrazol 300-400mg, triacontanol 0.5-1mg, compound sodium nitrophenolate 8-14mg, brassinolide 0.01-0.05mg and nucleotide 5-10 mg;
step two, field planting of water culture seedlings: fixedly planting the disinfected beautiful millettia root seedlings into the cultivation holes of the planting plates, and placing the planting plates on the cultivation grooves filled with the nutrient solution to ensure that one part of root systems of the beautiful millettia root seedlings is immersed in the nutrient solution and the other part of the root systems of the beautiful millettia root seedlings is not immersed in the nutrient solution;
step three, water culture management: after the rooting seedlings of beautiful millettia root are fixedly planted, the pH value of the nutrient solution is kept to be 5.8-6.5, and when the root system of the beautiful millettia root immersed in the nutrient solution is not more than 1/3, the nutrient solution is replaced; in the early stage and the middle stage of seedling exercising, the temperature in the daytime is 24-28 ℃, the temperature at night is 15-18 ℃, the relative humidity is 60-80%, the illumination intensity is 2000-; the temperature, humidity and illumination intensity in the later period of seedling hardening tend to be similar to those in the expected cultivation environment day by day.
2. The method for water culture seedling hardening of beautiful millettia root tissue culture seedlings according to claim 1, wherein in the second step, the method for sterilizing beautiful millettia root seedlings is to soak the beautiful millettia root seedlings in 0.2 percent by mass of carbendazim solution for 5 to 10 minutes.
3. The method for hydroponically hardening the beautiful millettia root tissue culture seedling according to claim 1, wherein the reagent for adjusting the pH is a nitric acid solution with a mass fraction of 5% and a sodium hydroxide solution with a mass fraction of 5%.
4. The water culture hardening device for the millettia speciosa champ tissue culture seedlings is characterized by comprising a water culture box for hardening the seedlings, a culture groove for providing nutrient solution for the tissue culture seedlings of the water culture box, a field planting plate for field planting of the tissue culture seedlings and a temperature and humidity illumination system for controlling the temperature, humidity and illumination in the water culture box, wherein the field planting plate is arranged above the culture groove;
the cultivation tank comprises a tank body, a plurality of electric telescopic rods for assisting the tank body to ascend and a spring set base for adjusting the distance between the bottom of the tank body and the planting plate according to the weight of nutrient solution in the tank body;
the spring group base comprises a bottom plate and a plurality of springs, the springs are the same in length, the springs are vertically and uniformly distributed on the bottom plate in parallel, one end of each spring is fixedly connected with the bottom plate, and the other end of each spring is fixedly connected with the bottom surface of the groove body;
the upper end of the tank body is open, a field planting plate is arranged on the upper opening of the tank body and is fixed with the water culture tank through a fixing piece, the distance between the bottom of the tank body and the field planting plate is changed through elastic deformation of a spring set base, when nutrient solution in the tank body increases, the weight of the tank body is increased, the spring set base is compressed, and the distance between the bottom of the tank body and the field planting plate is increased; when the nutrient solution in the tank body is reduced, the weight of the tank body is reduced, the spring group base gradually recovers to the original shape, and the distance between the bottom of the tank body and the planting plate is reduced; a drain outlet and a liquid inlet are arranged at the bottom of the tank body, and a drain electromagnetic valve and a liquid inlet electromagnetic valve are respectively arranged on the drain outlet and the liquid inlet;
a plurality of electric telescopic handle that supplementary cell body rose evenly distributed is in the below of cell body bottom, and electric telescopic handle's one end is fixed to be set up in the bottom of water planting case, and the other end can control the extension and support to press the cell body bottom and shrink and keep away from the cell body bottom.
5. The device for water culture seedling hardening of beautiful millettia root tissue culture seedling according to claim 4, characterized in that the side wall of the water culture box is provided with a door opening for moving in and pulling out the field planting plate, the door opening is correspondingly provided with a door plate, the drain opening and the liquid inlet are respectively detachably communicated with a drain pipe and a liquid inlet pipe, and the drain pipe and the liquid inlet pipe are hoses.
6. The device for water culture seedling hardening of beautiful millettia root tissue culture seedlings according to claim 4 or 5, characterized in that the fixing piece is a right-angled S-shaped connecting plate, the upper end and the lower end of the fixing piece are horizontal plates, the middle of the fixing piece is a vertical plate, and two ends of the vertical plate are respectively and vertically and fixedly connected with one end of the upper horizontal plate and one end of the lower horizontal plate; the other ends of the upper horizontal plates of the fixing pieces are fixedly connected with the side wall of the water culture box, the other ends of the lower horizontal plates of the fixing pieces extend towards the inside of the water culture box, and the extending planes of the lower horizontal plates of the fixing pieces are used for placing the planting plates; spaces formed among the upper horizontal plates, the vertical plates and the side walls of the water culture boxes of the plurality of fixing pieces are used for reserving spaces for the up-and-down movement of the walls of the tank body; and a vertical guide rail is arranged on the side wall of the water culture box and is used for enabling the wall of the tank body to slide up and down along the side wall of the water culture box.
7. The device for water culture and seedling exercising of beautiful millettia root tissue culture seedlings according to claim 6, wherein the plurality of springs have the same elastic coefficient, a weighing sensor is fixed on the bottom surface in the water culture box, and the bottom plate of the base of the spring group is pressed on the weighing sensor and is used for detecting the change condition of the weight of the nutrient solution in the tank body; the pollution discharge electromagnetic valve, the liquid inlet electromagnetic valve, the weighing sensor and the controller of the temperature and humidity illumination system are electrically connected.
8. The device for hydroponic seedling culture of beautiful millettia root tissue culture seedlings according to claim 7, wherein the temperature and humidity illumination system comprises a shading device, an illumination intensity sensor, a temperature sensor, a humidity sensor, an exhaust fan, a heating air pipe, a plant light supplement lamp and a controller;
the shading device is arranged on the outer wall of the water culture box and is used for shading the irradiation of natural light to the water culture box according to the illumination intensity requirement of the water culture box;
the plant light supplement lamp is arranged in the water culture box and used for controlling starting and adjusting the illumination intensity when the shading device cannot meet the illumination intensity requirement of the water culture box;
the illumination intensity sensor, the temperature sensor and the humidity sensor are arranged in the water culture box and used for detecting the illumination intensity, the temperature and the humidity in the water culture box and used as feedback signals for controlling the actions of the shading device, the plant light supplement lamp, the exhaust fan and the heating air pipe;
the water culture box is characterized in that the exhaust fan is arranged on the side wall of the water culture box and used for controlling the opening and closing and the rotating speed of the exhaust fan according to the temperature and humidity requirements of the water culture box;
the heating air pipe is arranged at the top of the water culture box and used for controlling the opening and closing of the heating air pipe according to the temperature requirement of the water culture box; the heating air pipe consists of a heating fan and a pipeline, and the outlet of the heating fan is communicated with the water culture box through the pipeline;
the shading device, the illumination intensity sensor, the temperature sensor, the humidity sensor, the exhaust fan, the plant light supplement lamp and the heating air pipe are respectively and electrically connected with the controller;
wherein, shade is collapsible puggaree, the water planting case wall is made by clear glass, is provided with a plurality of ventilation holes on the lateral wall of water planting case for natural draft and air exhauster convulsions adjust the temperature.
9. The device for hydroponically hardening the beautiful millettia root tissue culture seedlings according to claim 8, characterized in that a pH sensor and a liquid level sensor are further included in the tank body;
the pH sensor is arranged on the bottom side wall in the groove body;
the liquid level sensor is arranged below the planting plate and used for detecting the liquid level of the root system soaked in the nutrient solution;
the pH sensor and the liquid level sensor are respectively electrically connected with the controller, and the controller sends out corresponding control signals or alarm signals according to signals of the pH sensor and the liquid level sensor.
10. The device for water culture hardening-off of beautiful millettia root tissue culture seedlings according to any one of claims 7 to 9, characterized in that the control principle of controlling the root system of beautiful millettia root seedlings with specified length to be immersed in the nutrient solution by the device for water culture hardening-off is as follows: in the early stage of hardening, the electric telescopic rod is controlled to extend step by step to push the groove body to rise so as to immerse a part of the root system at the bottom of the beautiful millettia root-growing seedling into the nutrient solution; in the middle and later seedling hardening periods, the electric telescopic rod is shortened, the distance between the bottom of the groove body filled with nutrient solution and the planting plate is changed through the elastic deformation of the spring group base, when the nutrient solution in the groove body increases, the weight of the groove body is increased, the spring group base is compressed, and the distance between the bottom of the groove body and the planting plate is increased; when the nutrient solution in the groove body is reduced, the weight of the groove body is reduced, the base of the spring group gradually recovers to the original shape, and the distance between the bottom of the groove body and the planting plate is reduced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010615250.1A CN111758544A (en) | 2020-06-30 | 2020-06-30 | Water culture seedling hardening method and device for beautiful millettia root tissue culture seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010615250.1A CN111758544A (en) | 2020-06-30 | 2020-06-30 | Water culture seedling hardening method and device for beautiful millettia root tissue culture seedlings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111758544A true CN111758544A (en) | 2020-10-13 |
Family
ID=72724173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010615250.1A Pending CN111758544A (en) | 2020-06-30 | 2020-06-30 | Water culture seedling hardening method and device for beautiful millettia root tissue culture seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111758544A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114009269A (en) * | 2021-11-09 | 2022-02-08 | 贵州省黔桂茶业有限公司 | Planting method for improving quality and increasing yield of tea |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110078997A (en) * | 2009-12-31 | 2011-07-07 | 주식회사농심 | Cultivation method of green onion by using artificial lighting |
| US20150305259A1 (en) * | 2012-11-30 | 2015-10-29 | Galaxy Color S.R.L. | Device for domestic hydroponic cultivation |
| CN105010109A (en) * | 2015-06-30 | 2015-11-04 | 广西壮族自治区药用植物园 | Method for water planting grosvenor momordica |
| CN205682106U (en) * | 2016-06-20 | 2016-11-16 | 云南曲靖禹昌农牧科技开发有限公司 | A kind of essence control formula hydroponic box |
| CN107484576A (en) * | 2017-10-19 | 2017-12-19 | 绥阳瑞腾农业发展有限公司 | The device for raising seedlings of growing tomatoes |
| CN206776376U (en) * | 2017-04-28 | 2017-12-22 | 东北农业大学 | A kind of plant seedlings hydroponic device |
| CN107896960A (en) * | 2017-11-24 | 2018-04-13 | 天津职业技术师范大学 | A kind of water culture method |
| CN207927452U (en) * | 2017-12-19 | 2018-10-02 | 国家***桉树研究开发中心 | A kind of hydroponic device of the crop breeding with automatic dripping irrigation function |
| CN210275265U (en) * | 2019-08-09 | 2020-04-10 | 玉林师范学院 | Beautiful millettia root energy-saving seedling culture device |
| CN210808659U (en) * | 2019-11-05 | 2020-06-23 | 陈武胜 | Plant culture apparatus for biological teaching |
-
2020
- 2020-06-30 CN CN202010615250.1A patent/CN111758544A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110078997A (en) * | 2009-12-31 | 2011-07-07 | 주식회사농심 | Cultivation method of green onion by using artificial lighting |
| US20150305259A1 (en) * | 2012-11-30 | 2015-10-29 | Galaxy Color S.R.L. | Device for domestic hydroponic cultivation |
| CN105010109A (en) * | 2015-06-30 | 2015-11-04 | 广西壮族自治区药用植物园 | Method for water planting grosvenor momordica |
| CN205682106U (en) * | 2016-06-20 | 2016-11-16 | 云南曲靖禹昌农牧科技开发有限公司 | A kind of essence control formula hydroponic box |
| CN206776376U (en) * | 2017-04-28 | 2017-12-22 | 东北农业大学 | A kind of plant seedlings hydroponic device |
| CN107484576A (en) * | 2017-10-19 | 2017-12-19 | 绥阳瑞腾农业发展有限公司 | The device for raising seedlings of growing tomatoes |
| CN107896960A (en) * | 2017-11-24 | 2018-04-13 | 天津职业技术师范大学 | A kind of water culture method |
| CN207927452U (en) * | 2017-12-19 | 2018-10-02 | 国家***桉树研究开发中心 | A kind of hydroponic device of the crop breeding with automatic dripping irrigation function |
| CN210275265U (en) * | 2019-08-09 | 2020-04-10 | 玉林师范学院 | Beautiful millettia root energy-saving seedling culture device |
| CN210808659U (en) * | 2019-11-05 | 2020-06-23 | 陈武胜 | Plant culture apparatus for biological teaching |
Non-Patent Citations (1)
| Title |
|---|
| 郑先福: "《植物生长调节剂应用技术 第2版》", 30 June 2013, 中国农业大学出版 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114009269A (en) * | 2021-11-09 | 2022-02-08 | 贵州省黔桂茶业有限公司 | Planting method for improving quality and increasing yield of tea |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101699984B (en) | Artificial light cultivating method of dendrobium candidum | |
| CN102150538B (en) | Selenium-enriched paddy seedling and cultivation method thereof and produced organic selenium-enriched rice | |
| CN103880532B (en) | A kind of water planting implantation methods of Anoectochilus roxburghii | |
| CN111758544A (en) | Water culture seedling hardening method and device for beautiful millettia root tissue culture seedlings | |
| CN106962096A (en) | The implantation methods of selenium-enriched tea leaf | |
| CN104996130A (en) | Planting method for selenium-rich garlic | |
| CN107382509A (en) | A kind of Nutrition Soil preparation method | |
| CN1290404C (en) | Root system domestication of water planting flowers and trees, and plant cultivation method | |
| CN106258873A (en) | A kind of method utilizing biological floating bed plantation Herba Oenanthes Javanicae | |
| CN87207429U (en) | Plant cultivating container | |
| CN101496479A (en) | Method for preventing and treating clubroot disease on Cruciferae vegetables | |
| CN110122304A (en) | A kind of method of banana water planting hardening | |
| CN109329060A (en) | The method for carrying out tissue-culturing rapid propagation as explant to change brocade flower short-tube lycoris plateau | |
| CN104904577A (en) | Soilless cultivation method for cyclamen persicum | |
| CN105494105B (en) | A kind of peony tissue culture vessel seedling technology | |
| CN211793500U (en) | Water planting rubber grass system control device | |
| CN1303870C (en) | Culture method of Sinningia speciosa | |
| CN113632722A (en) | Method for water planting vegetables by utilizing biogas slurry | |
| CN113207688A (en) | Formula of mother liquor of hop tissue culture MS culture medium and rapid propagation technology | |
| CN110326500A (en) | The implantation methods of leaf vegetables selenium-rich | |
| CN207369737U (en) | A kind of removable seedling culture frame | |
| CN106069746B (en) | A kind of method of flat Europe hybrid hazel tissue-culturing rapid propagation nursery | |
| KR20150061061A (en) | Method for plantlet formation of Cypripedium macranthum Sw. using bioreactor | |
| CN109197504A (en) | Sugarcane test-tube seedling breeding nutrient soil and preparation method and application thereof | |
| CN108064697A (en) | A kind of efficient induction of Aralia mandshurica tissue-cultured seedling and method for transplanting |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201013 |