CN111714696A - 一种复合肌腱防粘连膜及制备方法 - Google Patents

一种复合肌腱防粘连膜及制备方法 Download PDF

Info

Publication number
CN111714696A
CN111714696A CN202010574129.9A CN202010574129A CN111714696A CN 111714696 A CN111714696 A CN 111714696A CN 202010574129 A CN202010574129 A CN 202010574129A CN 111714696 A CN111714696 A CN 111714696A
Authority
CN
China
Prior art keywords
membrane
adhesion
chitosan
collagen
composite tendon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010574129.9A
Other languages
English (en)
Inventor
李茹冰
丘海轶
赵月
吴云
李宇璐
魏照星
刘泗容
何楝楝
邹银萍
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chinese Academy Of Science Shenzhen Hospital, University of
Original Assignee
Chinese Academy Of Science Shenzhen Hospital, University of
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chinese Academy Of Science Shenzhen Hospital, University of filed Critical Chinese Academy Of Science Shenzhen Hospital, University of
Priority to CN202010574129.9A priority Critical patent/CN111714696A/zh
Publication of CN111714696A publication Critical patent/CN111714696A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0015—Electro-spinning characterised by the initial state of the material
    • D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
    • D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
    • D01F8/18—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from other substances
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
    • D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
    • D04H1/425—Cellulose series
    • D04H1/4258—Regenerated cellulose series
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
    • D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
    • D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H3/00—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length
    • D04H3/013—Regenerated cellulose series
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H3/00—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length
    • D04H3/02—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length characterised by the method of forming fleeces or layers, e.g. reorientation of yarns or filaments
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
    • D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
    • D06M15/15—Proteins or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/23—Carbohydrates
    • A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/04—Materials for stopping bleeding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
    • D06M2101/02—Natural fibres, other than mineral fibres
    • D06M2101/04—Vegetal fibres
    • D06M2101/06—Vegetal fibres cellulosic
    • D06M2101/08—Esters or ethers of cellulose
    • D—TEXTILES; PAPER
    • D10—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
    • D10B—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
    • D10B2401/00—Physical properties
    • D10B2401/13—Physical properties anti-allergenic or anti-bacterial
    • D—TEXTILES; PAPER
    • D10—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
    • D10B—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
    • D10B2509/00—Medical; Hygiene

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Transplantation (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Epidemiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Toxicology (AREA)
  • Mechanical Engineering (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Materials For Medical Uses (AREA)

Abstract

本发明提供了一种复合肌腱防粘连膜及制备方法，所述复合肌腱防粘连膜为壳聚糖修饰的纳米纤维膜，所述的纳米纤维膜上负载有胶原蛋白；所述的胶原蛋白负载量为5％～9％。该复合肌腱防粘连膜的制备方法，包括壳聚糖修饰的纳米纤维膜的制备、胶原蛋白的负载。本发明所制备得到的复合肌腱防粘连膜，既能够保证良好的生物贴合性，以更有效的促进内源性修复，又可以使复合肌腱防粘连膜具有优异的柔韧性和机械性能，增加粘连膜的使用寿命，减少肌腱周围组织形成的粘连。

Description

一种复合肌腱防粘连膜及制备方法
技术领域
本发明属于及生物材料领域，更具体地说，涉及一种复合肌腱防粘连膜及制备方法。
背景技术
肌腱损伤后粘连是手外科面临的难题之一，粘连是***纤维带与相邻的组织或器官结合在一起而形成的异常结构。外科手术后在组织愈合过程中很容易发生粘连现象，影响手术部位性能及功能的恢复。特别是肌腱部位，粘连问题非常常见，主要是由肌腱周围增生***膜的成纤维细胞接触肌腱而导致。而肌腱损伤恢复时间相对较长，且粘连现象会严重影响肌腱正常功能的恢复。
开发一种减少肌腱周围组织形成的粘连(即外源性愈合)，促进肌腱细胞本身的***增殖能力，不需形成粘连而愈合(即内源性愈合)的产品对于肌腱手术的成功至关重要。
经检索，关于防粘连生物膜的研究，现有技术已有相关的申请案公开，如中国专利申请号为201310358048.5，授权公告日期为2015.10.28的申请案公开了一种防粘连生物膜及其制备方法，是以SIS为支架材料，复合透明质酸、功能性蛋白和抗炎症药物而构成的干燥薄膜，呈浅白色至淡黄色半透明状，厚度为0.01～0.5mm。与现有技术相比，该申请的防粘连生物膜的生物相容性极好，既具有良好的物理屏障防粘连作用，又具有抗炎症、止血、促进纤维降解的作用，其效果明显优于已有的各类防粘连生物膜产品。该申请案的防粘连生物膜虽然具有优异的生物相容性，然而并未过多考虑整体材料的柔韧性和机械强度。
基于现有技术的缺陷，亟需发明一种新的既能有效防止粘连形成又不影响伤口的正常愈合，还具有一定的柔韧性和机械强度的复合肌腱防粘连膜。
发明内容
1.要解决的问题
针对现有技术中的防粘连膜难以同时保证优异的生物相容性以及机械性能的问题，本发明提供一种壳聚糖修饰的纳米纤维膜，所述的纳米纤维膜上还负载有胶原蛋白，将其作为复合肌腱防粘连膜不仅具有优异的生物相容性，还兼具优异的柔韧性、机械性能，利于肌腱损伤恢复。
2.技术方案
为了解决上述问题，本发明所采用的技术方案如下：
本发明提供了一种复合肌腱防粘连膜，所述的复合肌腱防粘连膜为壳聚糖修饰的纳米纤维膜，所述的纳米纤维膜上负载有胶原蛋白。
优选的，所述的胶原蛋白负载量为5％～9％。
优选的，所述的复合肌腱防粘连膜的制备方法，包括以下步骤：
1)壳聚糖修饰的纳米纤维膜的制备：将壳聚糖和纤维素衍生物溶解于混合溶剂，搅拌均匀后静置脱泡，得到静电纺丝溶液，然后进行静电纺丝，得到壳聚糖修饰的纳米纤维膜；
2)胶原蛋白的负载：将壳聚糖修饰的纳米纤维膜进行真空干燥处理，以去除纤维膜中残留的溶剂，然后将其置于胶原蛋白水解物溶液进行浸泡处理，随后冷冻干燥后得到复合肌腱防粘连膜。
优选的，所述步骤1)中静电纺丝溶液浓度为220～275g/L。
优选的，所述壳聚糖的分子量为2～50kDa，所述纤维素衍生物为纤维素乙酸酯。
优选的，所述步骤1)中所述壳聚糖和纤维素衍生物的质量比为1：2。
优选的，所述静电纺丝的工艺条件为：电压为18～24kV，针尖到滚筒的距离为10～15cm，溶液流速为0.5～1.0mL/h。
优选的，所述的混合溶剂为三氟乙酸、甲酸、乙酸、丙酮、N，N-二甲基甲酰胺、二甲基亚砜、六氟异丙醇、三氟乙醇和三氯甲烷中的任意两种或以上的混合物。
优选的，所述的胶原蛋白水解物溶液通过将水解胶原蛋白溶于水中得到，胶原蛋白水解物溶液的质量浓度为5％-15％。所述水解胶原蛋白购买自石家庄旭尔美生物科技有限公司。
优选的，所述步骤2)中浸泡处理的时间为18～25h。
3.有益效果
相比于现有技术，本发明的有益效果为：
(1)本发明提供的复合肌腱防粘连膜，以将壳聚糖和纤维素衍生物溶解于混合溶剂制备成静电纺丝溶液，然后进行静电纺丝，得到壳聚糖修饰的纳米纤维膜，在通过浸泡于胶原蛋白水解物溶液使其负载胶原蛋白，整体制备方法简单，既能够保证复合肌腱防粘连膜的良好的生物贴合性，以更有效的促进内源性修复，又可以使复合肌腱防粘连膜具有优异的柔韧性和机械性能，增加粘连膜的使用寿命，减少肌腱周围组织形成的粘连。
(2)本发明提供的复合肌腱防粘连膜，在纳米纤维膜层上负载有胶原蛋白，可以有效利用胶原蛋白所具有良好的贴合性及促进内源性愈合功能，纳米纤维膜层采用壳聚糖修饰的纳米纤维膜，可以有效增加整个材料的降解时间，起到有效的隔离作用，其中的有效成分壳聚糖同时还具有良好的止血抗菌功能，整个复合肌腱防粘连膜湿润后柔韧性好，另一方面，基于聚乳酸壳聚糖纳米纤维膜，具有高孔隙率，大比表面积特点，因此不仅具有更好的柔韧性和机械性能，还能利于透过营养物质，促进营养物质的运输。本发明的复合肌腱防粘连膜中各成分之间的协同作用将更加符合促进内源性愈合的机理，接近肌腱防粘连产品的理想作用。
附图说明
图1为实施例1中制备的复合肌腱防粘连膜的扫描电镜图(蛋白质负载量为5％)；
图2为实施例1中制备的复合肌腱防粘连膜动物实验的HE病理切片观察结果；
图3为实施例2中提供的复合肌腱防粘连膜的扫描电镜图(蛋白质负载量为6％)。
具体实施方式
需要说明的是，本说明书中所引用的如“上”、“下”、“左”、“右”、“中间”等用语，亦仅为便于叙述的明了，而非用以限定可实施的范围，其相对关系的改变或调整，在无实质变更技术内容下，当亦视为本发明可实施的范畴。
除非另有定义，本文所使用的所有的技术和科学术语与属于本发明的技术领域的技术人员通常理解的含义相同；本文所使用的术语“和/或”包括一个或多个相关的所列项目的任意的和所有的组合。
实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市售购买获得的常规产品。
如本文所使用，术语“约”用于提供与给定术语、度量或值相关联的灵活性和不精确性。本领域技术人员可以容易地确定具体变量的灵活性程度。
如本文所使用，术语“......中的至少一个”旨在与“......中的一个或多个”同义。例如，“A、B和C中的至少一个”明确包括仅A、仅B、仅C以及它们各自的组合。
浓度、量和其他数值数据可以在本文中以范围格式呈现。应当理解，这样的范围格式仅是为了方便和简洁而使用，并且应当灵活地解释为不仅包括明确叙述为范围极限的数值，而且还包括涵盖在所述范围内的所有单独的数值或子范围，就如同每个数值和子范围都被明确叙述一样。例如，约1至约4.5的数值范围应当被解释为不仅包括明确叙述的1至约4.5的极限值，而且还包括单独的数字(诸如2、3、4)和子范围(诸如1至3、2至4等)。相同的原理适用于仅叙述一个数值的范围，诸如“小于约4.5”，应当将其解释为包括所有上述的值和范围。此外，无论所描述的范围或特征的广度如何，都应当适用这种解释。
任何方法或过程权利要求中所述的任何步骤可以以任何顺序执行，并且不限于权利要求中提出的顺序。
下面结合具体实施例对本发明进一步进行描述。
实施例1
本发明中提供了一种复合肌腱防粘连膜，该复合肌腱防粘连膜为壳聚糖修饰的纳米纤维膜，所述的纳米纤维膜上负载有胶原蛋白，胶原蛋白负载量为5％。
上述复合肌腱防粘连膜的制备步骤如下：
1)壳聚糖修饰的纳米纤维膜的制备
将壳聚糖和为纤维素乙酸酯(二者质量比为质量比为1：2)溶解于混合溶剂，搅拌均匀后静置脱泡得到溶液浓度为220～275g/L静电纺丝溶液，然后进行静电纺丝；得到壳聚糖修饰的纳米纤维膜；所述壳聚糖的分子量为2～50kDa；
所述静电纺丝的工艺条件为：电压为18-24kV，针尖到滚筒的距离为10-15cm，溶液流速为0.5-1.0mL/h；
所述的混合溶剂为三氟乙酸、甲酸、乙酸、丙酮、N，N-二甲基甲酰胺、二甲基亚砜、六氟异丙醇、三氟乙醇和三氯甲烷中的任意两种或以上的混合物。
2)胶原蛋白的负载
将壳聚糖修饰的纳米纤维膜于37℃进行真空干燥处理，以去除纤维膜中残留的溶剂，然后将其置于胶原蛋白水解物溶液进行浸泡处理18-25h，随后，冷冻干燥后得到壳聚糖/纤维素-胶原蛋白复合的肌腱防粘连膜。
所述的胶原蛋白水解物溶液通过将水解胶原蛋白溶于水中得到，水解胶原蛋白的质量浓度为5％-15％；所述水解胶原蛋白购买自石家庄旭尔美生物科技有限公司。
本实施例中制备得到的复合肌腱防粘连膜(壳聚糖/纤维素-胶原蛋白纳米纤维膜)的力学性能测试数据如表1所示。该复合肌腱防粘连膜的扫描电镜图如图1所示。
表1壳聚糖/纤维素-胶原蛋白纳米纤维膜的力学性能测试数据
Figure BDA0002550411920000041
由表1可知，本发明制备的复合肌腱防粘连膜具有优异的柔韧性和机械性能。根据图1可知，本发明制备的负载胶原蛋白的复合肌腱防粘连膜孔隙结构发达，具有高孔隙率，大比表面积特点，因此不仅具有更好的柔韧性和机械性能，还能利于透过营养物质，实际使用过程中促进营养物质的运输。
为了验证本实施例制备的复合肌腱防粘连膜的实际的应用效果，进行动物实验，实验过程如下：
实验动物：成年SPF级SD大鼠，40只，雌雄各半，体重180～200g，购于广东省医学实验动物中心，适应性饲养3-7天后进行实验。
材料：手术器械、麻药(乌拉坦)、纱布、手术棉签、碘伏、酒精、手术铺巾、青霉素、带线缝合针(宁波医用缝针有限公司，尼龙3/0 40cm双针，1/2弧4×12)、可吸收电纺聚合膜
仪器设备方法：1)肌腱粘连动物模型的制备：麻醉动物后，动物双后肢剃毛，常规消毒铺巾，并将动物固定于手术台上。用碘伏消毒足部并用酒精脱碘，采用侧方切口暴露实验动物双侧第3趾深屈肌肌腱并手术横断肌腱中部50％，然后用带线缝合针用八字缝合法进行手术缝合肌腱断端，制备肌腱粘连模型，缝合皮肤；分别设置不同的组别，实验组使用实施例1制备的复合肌腱防粘连膜，对照组为未采取任何粘连膜的对比，并用石膏固定或用支具进行外固定。术后实验动物放回笼子单笼饲养。实验周期1周结束后，每组各取12只进行解剖行大体及组织学观察。
2)HE病理切片观察：取肌腱缝合口两侧0.5cm范围内的腱鞘和肌腱组织，常规石蜡包埋，行肌腱横切面切片，HE染色观察各时间点组织粘连，肌腱修复及炎性细胞浸润情况。
图2为实施例1中制备的复合肌腱防粘连膜动物实验的HE病理切片观察结果；其中图a为正常组：显示肌腱形态和结构均正常，未见炎性细胞浸润等异常情况；图b为对照组为术后三天的病理切片图，可以观察到开始出现粘连；图c为对照组为术后1-2周的病理切片图，可以观察到大量炎性细胞浸润现象，图d和图e为实验组术后1-2周的病理切片图，可以观察到少量炎性细胞浸润现象。
实施例2
本发明中提供了一种复合肌腱防粘连膜，该复合肌腱防粘连膜为壳聚糖修饰的纳米纤维膜，所述的纳米纤维膜上负载有胶原蛋白，胶原蛋白负载量为6％。图2为该实施例制备的复合肌腱防粘连膜的电镜图。
上述复合肌腱防粘连膜的制备步骤如下：
1)壳聚糖修饰的纳米纤维膜的制备
将壳聚糖(分子量为2～50kDa)和为纤维素乙酸酯(二者质量比为1：2)溶解于混合溶剂，搅拌均匀后静置脱泡得到溶液浓度为220-275g/L静电纺丝溶液，然后进行静电纺丝；得到壳聚糖修饰的纳米纤维膜；
所述静电纺丝的工艺条件为：电压为18-24kV，针尖到滚筒的距离为10-15cm，溶液流速为0.5-1.0mL/h；
所述的混合溶剂为三氟乙酸、甲酸、乙酸、丙酮、N，N-二甲基甲酰胺、二甲基亚砜、六氟异丙醇、三氟乙醇和三氯甲烷中的任意两种或以上的混合物。
2)胶原蛋白的负载
将壳聚糖修饰的纳米纤维膜于37℃进行真空干燥处理，以去除纤维膜中残留的溶剂，然后将其置于胶原蛋白水解物溶液进行浸泡处理18-25h，随后，冷冻干燥后得到壳聚糖/纤维素-胶原蛋白复合的肌腱防粘连膜。
所述的胶原蛋白水解物溶液通过将水解胶原蛋白溶于水中得到，水解胶原蛋白的质量浓度为5％-15％；所述水解胶原蛋白购买自石家庄旭尔美生物科技有限公司。
实施例3
本发明中提供了一种复合肌腱防粘连膜，该复合肌腱防粘连膜为壳聚糖修饰的纳米纤维膜，所述的纳米纤维膜上负载有胶原蛋白，胶原蛋白负载量为9％。
上述复合肌腱防粘连膜的制备步骤如下：
1)壳聚糖修饰的纳米纤维膜的制备
将壳聚糖(分子量为2～50kDa)和为纤维素乙酸酯(二者质量比为质量比为1：2)溶解于混合溶剂，搅拌均匀后静置脱泡得到溶液浓度为220-275g/L静电纺丝溶液，然后进行静电纺丝；得到壳聚糖修饰的纳米纤维膜；
所述静电纺丝的工艺条件为：电压为18-24kV，针尖到滚筒的距离为10-15cm，溶液流速为0.5-1.0mL/h；
所述的混合溶剂为三氟乙酸、甲酸、乙酸、丙酮、N，N-二甲基甲酰胺、二甲基亚砜、六氟异丙醇、三氟乙醇和三氯甲烷中的任意两种或以上的混合物。
2)胶原蛋白的负载
将壳聚糖修饰的纳米纤维膜于37℃进行真空干燥处理，以去除纤维膜中残留的溶剂，然后将其置于胶原蛋白水解物溶液进行浸泡处理18-25h，随后，冷冻干燥后得到壳聚糖/纤维素-胶原蛋白复合的肌腱防粘连膜。
所述的胶原蛋白水解物溶液通过将水解胶原蛋白溶于水中得到，水解胶原蛋白的质量浓度为5％-15％；所述水解胶原蛋白购买自石家庄旭尔美生物科技有限公司。

Claims (10)

1.一种复合肌腱防粘连膜，其特征在于：所述的复合肌腱防粘连膜为壳聚糖修饰的纳米纤维膜，所述的纳米纤维膜上负载有胶原蛋白。
2.根据权利要求1所述的复合肌腱防粘连膜，其特征在于：所述的胶原蛋白负载量为5％～9％。
3.权利要求1或2任意一项所述的复合肌腱防粘连膜的制备方法，其特征在于：包括以下步骤：
1)壳聚糖修饰的纳米纤维膜的制备：将壳聚糖和纤维素衍生物溶解于混合溶剂，搅拌均匀后静置脱泡，得到静电纺丝溶液，然后进行静电纺丝，得到壳聚糖修饰的纳米纤维膜；
2)胶原蛋白的负载：将壳聚糖修饰的纳米纤维膜进行真空干燥处理，以去除纤维膜中残留的溶剂，然后将其置于胶原蛋白水解物溶液进行浸泡处理，随后冷冻干燥后得到复合肌腱防粘连膜。
4.根据权利要求3所述的复合肌腱防粘连膜的制备方法，其特征在于：所述步骤1)中静电纺丝溶液浓度为220～275g/L。
5.根据权利要求4所述的复合肌腱防粘连膜的制备方法，其特征在于：所述壳聚糖的分子量为2～50kDa，所述纤维素衍生物为纤维素乙酸酯。
6.根据权利要求3所述的复合肌腱防粘连膜的制备方法，其特征在于：所述步骤1)中所述壳聚糖和纤维素衍生物的质量比为1：2。
7.根据权利要求6所述的复合肌腱防粘连膜的制备方法，其特征在于：所述静电纺丝的工艺条件为：电压为18～24kV，针尖到滚筒的距离为10～15cm，溶液流速为0.5～1.0mL/h。
8.根据权利要求7所述的复合肌腱防粘连膜的制备方法，其特征在于：所述的混合溶剂为三氟乙酸、甲酸、乙酸、丙酮、N，N-二甲基甲酰胺、二甲基亚砜、六氟异丙醇、三氟乙醇和三氯甲烷中的任意两种或以上的混合物。
9.根据权利要求6或7所述的复合肌腱防粘连膜的制备方法，其特征在于：所述的胶原蛋白水解物溶液通过将水解胶原蛋白溶于水中得到，胶原蛋白水解物溶液的质量浓度为5％-15％。
10.根据权利要求9所述的复合肌腱防粘连膜的制备方法，其特征在于：所述浸泡处理的时间为18～25h。
CN202010574129.9A 2020-06-22 2020-06-22 一种复合肌腱防粘连膜及制备方法 Pending CN111714696A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010574129.9A CN111714696A (zh) 2020-06-22 2020-06-22 一种复合肌腱防粘连膜及制备方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010574129.9A CN111714696A (zh) 2020-06-22 2020-06-22 一种复合肌腱防粘连膜及制备方法

Publications (1)

Publication Number Publication Date
CN111714696A true CN111714696A (zh) 2020-09-29

Family

ID=72569852

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010574129.9A Pending CN111714696A (zh) 2020-06-22 2020-06-22 一种复合肌腱防粘连膜及制备方法

Country Status (1)

Country Link
CN (1) CN111714696A (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113026199A (zh) * 2021-03-11 2021-06-25 北京化工大学 一种成分梯度化肌腱防粘连纤维膜及其制备方法
CN116005361A (zh) * 2022-12-29 2023-04-25 东莞盛翔新材料技术有限公司 一种纤维素-胶原蛋白复合纳米纤维膜的制备方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1944724A (zh) * 2006-10-11 2007-04-11 东华大学 胶原蛋白和壳聚糖复合纳米纤维及膜静电纺丝的制备方法
CN103405811A (zh) * 2013-08-16 2013-11-27 西安组织工程与再生医学研究所 一种防粘连生物膜及其制备方法
US20160367722A1 (en) * 2014-02-28 2016-12-22 The University Of Memphis Research Foundation Chitosan nanofiber compositions, compositions comprising modified chitosan, and methods of use
CN106390177A (zh) * 2016-09-28 2017-02-15 深圳先进技术研究院 一种壳聚糖基多层纳米纤维膜敷料及其制备方法和应用
CN109289075A (zh) * 2018-11-08 2019-02-01 武汉轻工大学 一种纤维素-胶原蛋白复合纳米纤维膜的制备方法及创伤敷料
CN110354316A (zh) * 2019-07-03 2019-10-22 天新福（北京）医疗器材股份有限公司 一种复合肌腱防粘连膜及其制备方法与应用

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1944724A (zh) * 2006-10-11 2007-04-11 东华大学 胶原蛋白和壳聚糖复合纳米纤维及膜静电纺丝的制备方法
CN103405811A (zh) * 2013-08-16 2013-11-27 西安组织工程与再生医学研究所 一种防粘连生物膜及其制备方法
US20160367722A1 (en) * 2014-02-28 2016-12-22 The University Of Memphis Research Foundation Chitosan nanofiber compositions, compositions comprising modified chitosan, and methods of use
CN106390177A (zh) * 2016-09-28 2017-02-15 深圳先进技术研究院 一种壳聚糖基多层纳米纤维膜敷料及其制备方法和应用
CN109289075A (zh) * 2018-11-08 2019-02-01 武汉轻工大学 一种纤维素-胶原蛋白复合纳米纤维膜的制备方法及创伤敷料
CN110354316A (zh) * 2019-07-03 2019-10-22 天新福（北京）医疗器材股份有限公司 一种复合肌腱防粘连膜及其制备方法与应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘伟: "《生物高分子材料及其应用研究》", 31 March 2018, 电子科技大学出版社 *
刘文等: "壳聚糖基复合膜的制备及预防术后肌腱粘连功能的研究", 《功能材料》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113026199A (zh) * 2021-03-11 2021-06-25 北京化工大学 一种成分梯度化肌腱防粘连纤维膜及其制备方法
CN116005361A (zh) * 2022-12-29 2023-04-25 东莞盛翔新材料技术有限公司 一种纤维素-胶原蛋白复合纳米纤维膜的制备方法
CN116005361B (zh) * 2022-12-29 2023-07-21 东莞盛翔新材料技术有限公司 一种纤维素-胶原蛋白复合纳米纤维膜的制备方法

Similar Documents

Publication Publication Date Title
Catto et al. Small diameter electrospun silk fibroin vascular grafts: Mechanical properties, in vitro biodegradability, and in vivo biocompatibility
Anton-Sales et al. Opportunities of bacterial cellulose to treat epithelial tissues
EP3072536B1 (en) Hydrophilic electrospinning biological composite stent material used for tissue regeneration and preparation method and application thereof
JP6407911B2 (ja) 止血組成物及び治療法
KR20100093516A (ko) 외과용 하이드로겔
Mirhaj et al. Preparation of a biomimetic bi-layer chitosan wound dressing composed of A-PRF/sponge layer and L-arginine/nanofiber
Rao et al. A multi-walled silk fibroin/silk sericin nerve conduit coated with poly (lactic-co-glycolic acid) sheath for peripheral nerve regeneration
CN110193098B (zh) 一种多层梯度生物膜及其制备方法
CN107551312B (zh) 一种絮状胶原止血纤维及其制备方法
CN115845141B (zh) 一种干态羊膜的制备方法及应用
CN111714696A (zh) 一种复合肌腱防粘连膜及制备方法
Rethinam et al. Preparation of absorbable surgical suture: Novel approach in biomedical application
Lu et al. Electrospun egg white/polyvinyl alcohol fiber dressing to accelerate wound healing
CN109381732A (zh) 负载生长因子小分子抑制剂的静电纺丝敷料、其制备方法及应用
Lin et al. Preparation and biocompatibility of electrospinning PDLLA/β-TCP/collagen for peripheral nerve regeneration
CN111317867A (zh) 一种神经导管及其制备方法
WO2023143337A1 (zh) 一种肩袖补片及其制备方法
Li et al. A native sericin wound dressing spun directly from silkworms enhances wound healing
CN113663137A (zh) 复合生物补片及其制备方法及应用
CN106139238A (zh) 一种壳聚糖胶原海绵敷料及其制备方法
Chen et al. Vapor-phase glutaraldehyde crosslinked waste protein-based nanofiber nonwovens as an environmentally friendly wound dressing
CN113288505A (zh) 基于ptmc的生物可吸收柔性弹性体的肠吻合支架及其制备方法
Kirubanandan et al. Regeneration of soft tissue using porous bovine collagen scaffold
Wang et al. Preclinical animal study of electrospun poly (l-lactide-co-caprolactone) and formulated porcine fibrinogen for full-thickness diabetic wound regeneration
CN115634307A (zh) 一种纤维膜及其制备方法和应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20200929

RJ01 Rejection of invention patent application after publication