CN111642389A - Breeding method of black gold paddy and illumination cultivation device thereof - Google Patents

Breeding method of black gold paddy and illumination cultivation device thereof Download PDF

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CN111642389A
CN111642389A CN202010549950.5A CN202010549950A CN111642389A CN 111642389 A CN111642389 A CN 111642389A CN 202010549950 A CN202010549950 A CN 202010549950A CN 111642389 A CN111642389 A CN 111642389A
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spring
sleeve
rod
guide rod
plate
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姜新
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture

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Abstract

The breeding method of the black golden valley comprises the following steps: step 1, hybridizing a spring triticale 76 serving as a female parent and a Zhoumai No. 18 wheat serving as a male parent to obtain an A1 generation; step 2, hybridizing the spring triticale 76 serving as a female parent and the Xiao Wei No. 6 wheat serving as a male parent to obtain an A2 generation; step 3, planting plants of A1 generation, backcrossing with Zhoumai No. 18 by utilizing A1, carrying out anther culture on hybrid A3 of the plants, irradiating by using a light culture device, and obtaining a germ line A3 through single plant selection; planting A3 generation, selecting fertile single plant for selfing to obtain A4 seed; step 4, planting A2 generation plants, carrying out first backcross with Xiaowein 6 wheat by utilizing A2, and obtaining a germ line A5 through single plant selection; planting A5 generation, selecting fertile single plant for selfing to obtain A6 seed; and 5, hybridizing the generations A4 and A6 to determine the strains. The invention aims to provide a breeding method of black golden paddy with high yield and good disease resistance and an illumination cultivation device thereof.

Description

Breeding method of black gold paddy and illumination cultivation device thereof
Technical Field
The invention belongs to the technical field of breeding, and particularly relates to a breeding method of black golden rice and an illumination cultivation device thereof.
Background
The black gold grain is one of wheat, barley subfamily, and wheat of the genus Lolium, Gramineae, and grows in the east longitude of 100 deg.C, and the north latitude of 38 deg.C. According to the research, the content of total flavone and trace elements such as calcium, iron, zinc and the like in the black golden millet strain is dozens of times higher than that of common crops. As more people and less land exist in China, the land area is continuously reduced, and the supply and demand contradiction of triticale is more and more prominent. To meet the increasing demand of black gold paddy consumption, the yield of black gold paddy must be increased and the disease resistance of the existing black gold paddy variety must be enhanced, and the most direct and effective way to increase the yield of black gold paddy and enhance the disease resistance is to breed the high-yield and ultrahigh-yield black gold paddy variety. The demand of people on rye food is met by breeding a new black golden valley variety with high yield, resistance and multiple disease resistance.
Disclosure of Invention
Therefore, the invention aims to solve the problems of low yield and poor disease resistance of the black golden millet in the prior art.
Therefore, the technical scheme adopted is that the breeding method of the black golden valley comprises the following steps:
step 1, hybridizing a spring triticale 76 serving as a female parent and a Zhoumai No. 18 wheat serving as a male parent to obtain an A1 generation;
step 2, hybridizing the spring triticale 76 serving as a female parent and the Xiao Wei No. 6 wheat serving as a male parent to obtain an A2 generation;
step 3, planting plants of generation A1, backcrossing with Zhoumai No. 18 by utilizing A1, carrying out anther culture on hybrid generation A3, irradiating 15 days after callus grows out by using an illumination culture device for 48-72 hours, transplanting the plants to a field, and obtaining a germ line A3 by single plant selection; planting A3 generation, selecting fertile single plants for selfing, and harvesting purple black grains to obtain A4 seeds;
step 4, planting plants of A2 generation, carrying out first backcross with wheat of Xiao Wei No. 6 by utilizing A2, carrying out anther culture on hybrid A5, irradiating 20 days after callus grows out by using an illumination culture device for 48-72 hours, transplanting to a field, and obtaining a germ line A5 through single plant selection; planting A5 generation, selecting fertile single plants for selfing, and harvesting purple black grains to obtain A6 seeds;
and 5, hybridizing the generations A4 and A6, planting the hybrid seeds at a row spacing of 30 cm and a plant spacing of 5 cm, and harvesting the hybrid seeds of the purple black seeds when the seeds are mature to determine the hybrid seeds as strains.
Preferably, the step 5 comprises:
anther culture is carried out on the hybrid A4 and A6, the callus is irradiated by a light culture device for 48-72 hours 10-30 days after the growth, and the callus is transplanted to a field respectively.
Preferably, wheat is used instead of Zhouma No. 18.
An illumination cultivation device is suitable for the breeding method of the black golden paddy, and comprises the following steps:
the fixing plate is provided with a first limiting plate and a second limiting plate respectively, a first guide rod is arranged in the horizontal direction, one end of the first guide rod is connected with the first limiting plate, and the other end of the first guide rod is connected with the second limiting plate;
the first sleeve is sleeved on the first guide rod and can reciprocate left and right along the first guide rod;
the third limiting plate is arranged below the first sleeve, a second guide rod is arranged in the vertical direction, one end of the second guide rod is connected with the outer wall of the first sleeve, and the other end of the second guide rod is connected with the third limiting plate;
the second sleeve is sleeved on the second guide rod and can reciprocate up and down along the second guide rod, and a lamp is arranged on the outer wall of the second sleeve.
Preferably, a driving device is further disposed on the fixing plate, and the driving device includes:
the motor is arranged on the fixing plate, and an output shaft of the motor is connected with the outer wall of the third sleeve;
the right end of the third guide rod is connected with a first connecting plate, one end of the first connecting plate is vertically connected with one end of a second connecting plate, and the second connecting plate is positioned above the first connecting plate;
one end of the connecting rod is rotatably connected with the second connecting plate, and the other end of the connecting rod is connected with the bottom end of the second sleeve;
the first spring is sleeved on the third guide rod, one end of the first spring is connected with the third sleeve, and the other end of the first spring is connected with the first connecting plate.
Preferably, a glass cover is arranged on the fixing plate, and the lamp is located in the glass cover.
Preferably, both ends are equipped with second spring and third spring respectively about first guide bar, second spring one end with first limiting plate connection, the second spring other end is in unsettled state, third spring one end with second limiting plate connection, the third spring other end is in unsettled state.
Preferably, the upper end and the lower end of the second guide rod are respectively sleeved with a fourth spring and a fifth spring, one end of the fourth spring is connected with the outer wall of the first sleeve, the other end of the fourth spring is in a suspended state, one end of the fifth spring is connected with the third limiting plate, and the other end of the fifth spring is in a suspended state.
Preferably, a light intensity control device is arranged on the outer wall of the second sleeve, and the light intensity control device comprises:
one end of the fixed shaft is connected with the outer wall of the second sleeve, the other end of the fixed shaft is rotatably connected with a disc, and the disc can rotate around the fixed shaft;
the arc-shaped plate is sleeved on the disc and fixedly connected with the disc, one end of the arc-shaped plate is provided with a deflector rod, the deflector rod extends along the radial direction of the disc, the other end of the arc-shaped plate is connected with one end of a connecting column, the other end of the connecting column is connected with a light screen, and the light screen can shield the lamp;
the arc-shaped convex block is arranged on the disc and is positioned between the two ends of the arc-shaped plate;
the fixed block is arranged on the outer wall of the second sleeve, one end of the fixed block is provided with a guide hole, a sixth spring is arranged in the guide hole, one end of the sixth spring is communicated with the bottom wall of the guide hole, the other end of the sixth spring is connected with one end of a moving rod, the other end of the moving rod extends out of the fixed block and is clamped between the arc-shaped convex block and the end face of the arc-shaped plate, and one end of the moving rod, far away from the sixth spring, is an arc face;
the first blocking rod is arranged above the shifting rod, the first blocking rod is connected with one end of a first L-shaped supporting rod, and the other end of the first L-shaped supporting rod is connected with the outer wall of the second guide rod;
and the second stop lever is arranged below the shifting lever, the second stop lever is connected with one end of a second L-shaped supporting rod, and the other end of the second L-shaped supporting rod is connected with the outer wall of the second guide rod.
Preferably, one end of the third guide rod, which is far away from the first connecting plate, is provided with a limiting block.
The technical scheme of the invention has the following advantages:
1. the triticale bred by the breeding method provided by the invention is named as black gold paddy, the yield and the disease resistance agronomic characteristics are outstanding, and the contents of trace elements and amino acids are far higher than those of common triticale. The Ca content of the black golden valley and the black wheat is 3990ug/g, the Zn content is 448ug/g, the Fe content is 1580ug/g, and the Se content is 0.390 ug/g.
2. Because the black character of the black golden millet is controlled by a single gene, the black is dominant, the grains are black, and the black character is easy to reappear and not easy to lose during separation after generation. The backcross method has stable and fast agronomic characters and is easy to breed new varieties. By adopting conventional breeding, anther cultivation, backcross and hybridization technologies and combining a slow radiation irradiation method, a plurality of beneficial genes are polymerized, and the callus with vigorous progeny is slowly irradiated, so that the optimization probability is improved.
3. Zhoumai No. 18 and Xiaoyan No. 6 are high-yield varieties with more ears, large grains and strong capacities of resisting wheat rust, leaf blight, powdery mildew, scab and the like. Backcross and backcross are carried out by taking Zhoumai No. 18 and Xiaoyan No. 6 as parents, so as to breed a new black wheat variety with similar agronomic characters to the Zhoumai No. 18 and Xiaoyan No. 6, and the bred strain has the characteristics of high yield, strong disease resistance and the like.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the structure of an illumination cultivation apparatus of the present invention;
FIG. 2 is a schematic view of the structure of the driving device of the present invention;
FIG. 3 is a schematic view of a displacement adjustment mechanism according to the present invention;
FIG. 4 is a schematic structural diagram of a light intensity control device according to the present invention.
Wherein, 1-a fixed plate, 2-a first limit plate, 3-a second limit plate, 4-a first guide rod, 5-a first sleeve, 6-a third limit plate, 7-a second guide rod, 8-a second sleeve, 9-a lamp, 10-a motor, 11-a sleeve, 12-a third guide rod, 13-a first connecting plate, 14-a second connecting plate, 15-a connecting rod, 16-a first spring, 17-a glass cover, 18-a second spring, 19-a third spring, 20-a fourth spring, 21-a fifth spring, 22-a fixed shaft, 23-a disc, 24-an arc plate, 25-a deflector rod, 26-a connecting column, 27-a shading plate, 28-an arc lug, 29-a fixed block and 30-a guide hole, 31-a sixth spring, 32-a moving rod, 33-a limiting block, 34-a first gear rod, 35-a second gear rod, 36-a first L-shaped supporting rod and 37-a second L-shaped supporting rod.
Detailed Description
The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.
The invention provides a breeding method of Heijinggu, which comprises the steps of hybridizing spring triticale 76 serving as a female parent and wheat varieties Zhoumai No. 18 wheat and Xiaowei No. 6 wheat with high disease resistance level serving as male parents to obtain an A1 generation and an A2 generation respectively; planting plants of A1 generation, identifying the disease resistance of each combination of A1 generation, backcrossing the single plants with high resistance and similar agronomic and yield characters with Zhoumai No. 18 with recurrent parent Hui No. 18, harvesting seeds of A3 generation, performing indoor generation-adding accelerated selfing purification, and shortening the innovation period. And (3) carrying out young embryo culture on the obtained hybrid A3 indoors, wherein the specific process of the young embryo culture is as follows: taking seeds 14 days after pollination, sterilizing the seeds with 70% alcohol for 20 seconds, washing the seeds with sterile water for 3 times, peeling off embryos on a sterile operating platform, inoculating the embryos onto an induction culture medium, irradiating the embryos for 48 to 72 hours by using a light cultivation device 15 days after callus growth, increasing variation and improving the optimization probability, transplanting the obtained regenerated plants into a field in autumn after doubling, selecting fertile single plants for selfing in A3 generation, and harvesting purplish black grains to obtain A4 seeds. Planting plants of A2 generation, identifying the disease resistance of each combination of A2 generation, backcrossing the single plants which have high resistance and have agronomic and yield characters similar to those of the small weir No. 6 with the recurrent parent in the small weir No. 6, harvesting seeds of A5 generation, performing indoor generation-adding accelerated selfing purification, and shortening the innovation period. And (3) carrying out young embryo culture on the obtained hybrid A5 indoors, wherein the specific process of the young embryo culture is as follows: taking seeds 14 days after pollination, sterilizing the seeds with 70% alcohol for 20 seconds, washing the seeds with sterile water for 3 times, peeling off embryos on a sterile operating platform, inoculating the embryos onto an induction culture medium, irradiating the embryos with Co60 at the dose of 300 rad/hour for 15 days after callus growth, wherein the irradiation time is 48-72 hours, so that the variation is increased, the optimization probability is improved, the obtained regenerated plants are doubled and transplanted into a field in autumn, fertile single plants are selected for selfing in A5 generations, and black grains are harvested to obtain A6 seeds. The planting method comprises the steps of carrying out hybridization on A4 and A6, planting the seeds at a row spacing of 30 cm and a plant spacing of 5 cm, harvesting hybrid seeds of purple black seeds when the seeds are mature, and determining the hybrid seeds as strains.
In one embodiment, an illumination cultivation device is suitable for a breeding method of black gold grains, as shown in fig. 1-2, and comprises:
the device comprises a fixed plate 1, wherein a first limiting plate 2 and a second limiting plate 3 are respectively arranged on the fixed plate 1, a first guide rod 4 is horizontally arranged, one end of the first guide rod 4 is connected with the first limiting plate 2, and the other end of the first guide rod 4 is connected with the second limiting plate 3;
the first sleeve 5 is sleeved on the first guide rod 4, and the first sleeve 5 can reciprocate left and right along the first guide rod 4;
the third limiting plate 6 is arranged below the first sleeve 5, the second guide rod 7 is arranged in the vertical direction, one end of the second guide rod 7 is connected with the outer wall of the first sleeve 5, and the other end of the second guide rod 7 is connected with the third limiting plate 6;
and the second sleeve 8 is sleeved on the second guide rod 7, the second sleeve 8 can move up and down along the second guide rod 7, a lamp 9 is arranged on the outer wall of the second sleeve 8, and a battery is arranged on the second sleeve 8 to provide electric energy for the lamp 9.
The driving device includes:
the motor 10 is arranged on the fixing plate 1, and an output shaft of the motor 10 is connected with the outer wall of the third sleeve 11;
the third guide rod 12 is arranged in the third sleeve 11, the right end of the third guide rod 12 is connected with the first connecting plate 13, one end of the first connecting plate 13 is vertically connected with one end of the second connecting plate 14, and the second connecting plate 14 is positioned above the first connecting plate 13;
one end of the connecting rod 15 is rotatably connected with the second connecting plate 14, and the other end of the connecting rod 15 is connected with the bottom end of the second sleeve 8;
the first spring 16 is sleeved on the third guide rod 12, one end of the first spring 16 is connected with the third sleeve 11, and the other end of the first spring 16 is connected with the first connecting plate 13.
The working principle and the beneficial technical effects of the technical scheme are as follows: fixing the fixing plate 1 in a cultivation shed, when the black golden valley needs to be cultivated by illumination, starting the motor 10, driving the third sleeve 11 to rotate by the motor 10, under the action of the first spring 16, pushing the third guide rod 12 to reciprocate along the third sleeve 11, driving the first connecting plate 13 and the connecting plate 14 to move by the third guide rod 12, driving the connecting rod 15 and the second sleeve 8 by the connecting plate 14, enabling the second sleeve 8 to reciprocate along the second guide rod 7, enabling the first sleeve 5 to reciprocate along the first guide rod 4, so that the second sleeve 8 moves on the fixing plate 1 for one circle along a rectangular track when the third sleeve 11 rotates for one circle, the light on the second sleeve 8 can provide illumination conditions for the black golden valley, because the light 9 also moves along the rectangular track, the illumination conditions at each point are different in position and illumination angle and are closer to the illumination conditions of sunlight, the cultivated black gold paddy can be more easily adapted to the natural environment, and the environmental adaptability of the black gold paddy is improved.
In one embodiment, as shown in fig. 1, a glass cover 17 is disposed on the fixing plate 1, the lamp 9 is located in the glass cover 17, and the glass cover 17 serves to protect the lamp 9.
In one embodiment, as shown in fig. 3, a second spring 18 and a third spring 19 are respectively sleeved at left and right ends of the first guide rod 4, one end of the second spring 18 is connected with the first limiting plate 2, the other end of the second spring 18 is in a suspended state, one end of the third spring 19 is connected with the second limiting plate 3, and the other end of the third spring 19 is in a suspended state.
The working principle and the beneficial technical effects of the technical scheme are as follows: the second spring 18 and the third spring 19 play a role of limiting the left and right displacement of the first sleeve 5, and the power for the left and right movement of the first sleeve 5 comes from the elastic force of the first spring 16 and the centrifugal force of the first connecting plate 13, so that the pressure applied to the second spring 18 and the third spring 19 can be changed by changing the rotation speed of the motor and changing the centrifugal force, thereby playing a role of adjusting the left and right limit displacement of the first sleeve 5.
In one embodiment, as shown in fig. 3, a fourth spring 20 and a fifth spring 21 are respectively sleeved at the upper end and the lower end of the second guide rod 7, one end of the fourth spring 20 is connected to the outer wall of the first sleeve 5, the other end of the fourth spring 20 is in a suspended state, one end of the fifth spring 21 is connected to the third limiting plate 6, and the other end of the fifth spring 21 is in a suspended state.
The working principle and the beneficial technical effects of the technical scheme are as follows: the rotational speed of change motor changes centrifugal force, just can change the pressure of applying at fourth spring 20 and fifth spring 21 to play the effect of adjusting the upper and lower extreme displacement of second sleeve 8, therefore, the regulation of the rectangle movement track size of second sleeve 8 just can be realized through the rotational speed that changes the motor, thereby satisfies the demand of the different illumination conditions of black golden valley.
In one embodiment, as shown in fig. 4, the second sleeve 8 is provided with a light intensity control device on the outer wall, and the light intensity control device comprises:
one end of the fixed shaft 22 is connected with the outer wall of the second sleeve 8, the other end of the fixed shaft 22 is rotatably connected with a disc 23, and the disc 23 can rotate around the fixed shaft 22;
the arc-shaped plate 24 is sleeved on the disc 23, the arc-shaped plate 24 is fixedly connected with the disc 23, one end of the arc-shaped plate 24 is provided with a shift lever 25, the shift lever 25 extends along the radial direction of the disc 23, the other end of the arc-shaped plate 24 is connected with one end of a connecting column 26, the other end of the connecting column 26 is connected with a light shielding plate 27, and the light shielding plate 27 can shield the lamp 9;
an arc-shaped projection 28 arranged on the disc 23, wherein the arc-shaped projection 28 is positioned between two ends of the arc-shaped plate 24;
the fixed block 29 is arranged on the outer wall of the second sleeve 8, one end of the fixed block 29 is provided with a guide hole 30, a sixth spring 31 is arranged in the guide hole 30, one end of the sixth spring 31 is communicated with the bottom wall of the guide hole 30, the other end of the sixth spring 31 is connected with one end of a moving rod 32, the other end of the moving rod 32 extends out of the fixed block 29 and is clamped between the arc-shaped bump 28 and the end face of the arc-shaped plate 24, and one end, far away from the sixth spring 31, of the moving rod 32 is an arc face;
the first gear lever 34 is arranged above the shift lever 25, the first gear lever 34 is connected with one end of a first L-shaped support rod 36, and the other end of the first L-shaped support rod 36 is connected with the outer wall of the second guide rod 7;
and the second gear lever 35 is arranged below the shift lever 25, the second gear lever 35 is connected with one end of a second L-shaped support rod 37, and the other end of the second L-shaped support rod 37 is connected with the outer wall of the second guide rod 7.
The working principle and the beneficial technical effects of the technical scheme are as follows: when the second sleeve 8 moves upwards, the light shielding plate 27 shields the lamp 9, when the shift lever 25 moves to the first blocking rod 34, the shift lever 25 is shifted to rotate anticlockwise to drive the arc-shaped plate 24, the disc 23 and the arc-shaped convex block 28 to rotate anticlockwise, the movable rod 32 overcomes the elasticity of the sixth spring 31 and moves into the guide hole 30 by extruding the movable rod 32 through the arc-shaped convex block 28, when the upper end of the movable rod 32 moves to a position between the arc-shaped convex block 28 and the left end face of the arc-shaped plate 24, the disc 23 is fixed, due to the rotation of the disc 23, the light shielding plate 27 is far away from the lamp 9, and at the moment, the lamp 9 provides illumination for black golden valley plants; when the second sleeve 8 moves downwards, the second shift rod 35 will shift the shift lever 25 accordingly, so that the light shielding plate 27 shields the lamp 9 again, and the lamp 9 cannot provide illumination for the black paddy plant. Thus, when the lamp 9 moves along the rectangular trajectory, the lamp 9 is in the shielding state for half of the time, and the lamp 9 is in the opening state for the other half of the time, thereby simulating the alternate lighting conditions of day and night under the sunshine condition and providing the lighting conditions closer to the natural condition.
In one embodiment, as shown in fig. 2, a stopper 33 is disposed at an end of the third guiding rod 12 away from the first connecting plate 13, and the stopper 33 functions to limit the displacement of the third guiding rod 12 and prevent the third guiding rod 12 from moving out of the third sleeve 11.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims (10)

1. A breeding method of black gold paddy is characterized by comprising the following steps:
step 1, hybridizing a spring triticale 76 serving as a female parent and a Zhoumai No. 18 wheat serving as a male parent to obtain an A1 generation;
step 2, hybridizing the spring triticale 76 serving as a female parent and the Xiao Wei No. 6 wheat serving as a male parent to obtain an A2 generation;
step 3, planting plants of generation A1, backcrossing with Zhoumai No. 18 by utilizing A1, carrying out anther culture on hybrid generation A3, irradiating 15 days after callus grows out by using an illumination culture device for 48-72 hours, transplanting the plants to a field, and obtaining a germ line A3 by single plant selection; planting A3 generation, selecting fertile single plants for selfing, and harvesting purple black grains to obtain A4 seeds;
step 4, planting plants of A2 generation, carrying out first backcross with wheat of Xiao Wei No. 6 by utilizing A2, carrying out anther culture on hybrid A5, irradiating 20 days after callus grows out by using an illumination culture device for 48-72 hours, transplanting to a field, and obtaining a germ line A5 through single plant selection; planting A5 generation, selecting fertile single plants for selfing, and harvesting purple black grains to obtain A6 seeds;
and 5, hybridizing the generations A4 and A6, planting the hybrid seeds at a row spacing of 30 cm and a plant spacing of 5 cm, and harvesting the hybrid seeds of the purple black seeds when the seeds are mature to determine the hybrid seeds as strains.
2. A breeding method of Heijinggu as claimed in claim 1, wherein said step 5 includes:
anther culture is carried out on the hybrid A4 and A6, the callus is irradiated by a light culture device for 48-72 hours 10-30 days after the growth, and the callus is transplanted to a field respectively.
3. A breeding method of Heijinggu according to claim 1, characterized in that the Zhoumai No. 18 is replaced by wheat.
4. An illumination cultivation device suitable for the breeding method of the black golden valley according to any one of claims 1 to 3, comprising:
the fixing plate (1) is provided with a first limiting plate (2) and a second limiting plate (3) respectively, a first guide rod (4) is arranged in the horizontal direction, one end of the first guide rod (4) is connected with the first limiting plate (2), and the other end of the first guide rod (4) is connected with the second limiting plate (3);
the first sleeve (5) is sleeved on the first guide rod (4), and the first sleeve (5) can reciprocate left and right along the first guide rod (4);
the third limiting plate (6) is arranged below the first sleeve (5), the second guide rod (7) is arranged in the vertical direction, one end of the second guide rod (7) is connected with the outer wall of the first sleeve (5), and the other end of the second guide rod (7) is connected with the third limiting plate (6);
and the second sleeve (8) is sleeved on the second guide rod (7), the second sleeve (8) can move up and down along the second guide rod (7), and a lamp (9) is arranged on the outer wall of the second sleeve (8).
5. An illumination cultivation device according to claim 4, characterized in that a driving device is further provided on the fixing plate (1), said driving device comprises:
the motor (10) is arranged on the fixing plate (1), and an output shaft of the motor (10) is connected with the outer wall of the third sleeve (11);
the third guide rod (12) is arranged in the third sleeve (11), the right end of the third guide rod (12) is connected with the first connecting plate (13), one end of the first connecting plate (13) is vertically connected with one end of the second connecting plate (14), and the second connecting plate (14) is positioned above the first connecting plate (13);
one end of the connecting rod (15) is rotatably connected with the second connecting plate (14), and the other end of the connecting rod (15) is connected with the bottom end of the second sleeve (8);
the first spring (16) is sleeved on the third guide rod (12), one end of the first spring (16) is connected with the third sleeve (11), and the other end of the first spring (16) is connected with the first connecting plate (13).
6. An illumination cultivation device according to claim 4, characterized in that a glass cover (17) is arranged on the fixing plate (1), and the lamp (9) is located in the glass cover (17).
7. An illumination cultivation device as claimed in claim 4, wherein the left and right ends of the first guiding rod (4) are respectively sleeved with a second spring (18) and a third spring (19), one end of the second spring (18) is connected with the first limiting plate (2), the other end of the second spring (18) is in a suspended state, one end of the third spring (19) is connected with the second limiting plate (3), and the other end of the third spring (19) is in a suspended state.
8. An illumination cultivation device as claimed in claim 4, wherein the upper and lower ends of the second guiding rod (7) are respectively sleeved with a fourth spring (20) and a fifth spring (21), one end of the fourth spring (20) is connected with the outer wall of the first sleeve (5), the other end of the fourth spring (20) is in a suspended state, one end of the fifth spring (21) is connected with the third limiting plate (6), and the other end of the fifth spring (21) is in a suspended state.
9. An illumination cultivating device according to claim 4, wherein the second sleeve (8) is provided with light intensity control means on its outer wall, said light intensity control means comprising:
one end of the fixed shaft (22) is connected with the outer wall of the second sleeve (8), the other end of the fixed shaft (22) is rotatably connected with a disc (23), and the disc (23) can rotate around the fixed shaft (22);
the lamp holder is characterized by comprising an arc-shaped plate (24), the arc-shaped plate (24) is sleeved on the disc (23), the arc-shaped plate (24) is fixedly connected with the disc (23), a shifting rod (25) is arranged at one end of the arc-shaped plate (24), the shifting rod (25) extends along the radial direction of the disc (23), the other end of the arc-shaped plate (24) is connected with one end of a connecting column (26), the other end of the connecting column (26) is connected with a light screen (27), and the light screen (27) can shield the lamp (9);
the arc-shaped projection (28) is arranged on the disc (23), and the arc-shaped projection (28) is positioned between two ends of the arc-shaped plate (24);
the fixed block (29) is arranged on the outer wall of the second sleeve (8), one end of the fixed block (29) is provided with a guide hole (30), a sixth spring (31) is arranged in the guide hole (30), one end of the sixth spring (31) is communicated with the bottom wall of the guide hole (30), the other end of the sixth spring (31) is connected with one end of a moving rod (32), the other end of the moving rod (32) extends out of the fixed block (29) and is clamped between the arc-shaped convex block (28) and the end face of the arc-shaped plate (24), and one end, far away from the sixth spring (31), of the moving rod (32) is an arc face;
the first gear lever (34) is arranged above the shifting lever (25), the first gear lever (34) is connected with one end of a first L-shaped supporting rod (36), and the other end of the first L-shaped supporting rod (36) is connected with the outer wall of the second guide rod (7);
the second gear lever (35) is arranged below the shifting lever (25), the second gear lever (35) is connected with one end of a second L-shaped supporting rod (37), and the other end of the second L-shaped supporting rod (37) is connected with the outer wall of the second guide rod (7).
10. An illumination cultivation device according to claim 5, characterized in that the end of the third guiding rod (12) away from the first connecting plate (13) is provided with a stopper (33).
CN202010549950.5A 2020-06-16 2020-06-16 Breeding method of black gold paddy and illumination cultivation device thereof Pending CN111642389A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101735948A (en) * 2010-01-11 2010-06-16 新奥科技发展有限公司 Method and device for cultivating microalgae
CN102405796A (en) * 2011-08-12 2012-04-11 北京农业智能装备技术研究中心 Seedling raising device capable of regulating intensity and angle of received light
CN106125806A (en) * 2016-08-30 2016-11-16 楼彦卿 A kind of plant cultivating device and comprise its plant cultivation long distance control system
CN108184657A (en) * 2018-02-27 2018-06-22 姜新 A kind of breeding method of dark fund paddy

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101735948A (en) * 2010-01-11 2010-06-16 新奥科技发展有限公司 Method and device for cultivating microalgae
CN102405796A (en) * 2011-08-12 2012-04-11 北京农业智能装备技术研究中心 Seedling raising device capable of regulating intensity and angle of received light
CN106125806A (en) * 2016-08-30 2016-11-16 楼彦卿 A kind of plant cultivating device and comprise its plant cultivation long distance control system
CN108184657A (en) * 2018-02-27 2018-06-22 姜新 A kind of breeding method of dark fund paddy

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