CN111575161B - Multi-angle overturning reaction bin device for gene synthesis - Google Patents

Multi-angle overturning reaction bin device for gene synthesis Download PDF

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CN111575161B
CN111575161B CN202010324102.4A CN202010324102A CN111575161B CN 111575161 B CN111575161 B CN 111575161B CN 202010324102 A CN202010324102 A CN 202010324102A CN 111575161 B CN111575161 B CN 111575161B
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sleeve
reaction bin
rotate
bevel gear
reaction
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CN111575161A (en
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雍金贵
施荣辉
黄晓峰
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General Biology Anhui Co ltd
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Abstract

The invention discloses a multi-angle overturning reaction bin device for gene synthesis, which comprises a first sleeve and a mounting seat, wherein the mounting seat is rotatably arranged at one end of the top of a loading box. The one end rotation of mount pad is installed first sleeve, and the driving gear is installed along the circumferencial direction in the outside of first sleeve, and mount pad one end is installed a plurality of carrier bar in the outside rotation of first sleeve, and the driven gear is installed along the circumferencial direction in the outside of carrier bar, and driven gear is connected with the driving gear meshing. Before use, adjust the quantity of annular piece in each balancing weight for the weight is different between each balancing weight, and then makes the rotation of different weight balancing weights can make the atress of mount pad all directions unbalanced, and then can obtain rocking in making the reaction storehouse rotate, can overturn different angles. Meanwhile, the rotating rod can drive the mounting seat to rotate, so that the overturning angle of the reaction bin is changed, the range of the angle change is enlarged, and different working requirements can be met.

Description

Multi-angle overturning reaction bin device for gene synthesis
Technical Field
The invention relates to a reaction chamber device, in particular to a multi-angle overturning reaction chamber device for gene synthesis, and belongs to the field of gene synthesis application.
Background
The gene synthesis is a commonly used technology in bioengineering, and a commonly used reaction bin device is used for processing the gene synthesis, so that the gene synthesis is more stable and accurate.
However, the existing gene synthesis reaction bin device still has certain defects in use. Most of the existing gene synthesis reaction bin devices are in a static state, and cannot be overturned at multiple angles in use, so that the gene synthesis process is not thorough enough and not comprehensive enough, and the synthesis efficiency is affected. The position of the reaction bin is inconvenient to adjust the angle, and the limitation in use is large.
Disclosure of Invention
The invention aims to provide a multi-angle overturning reaction bin device for gene synthesis, which can solve the problems that the existing gene synthesis reaction bin device is in a static state and cannot be overturned at multiple angles in use, so that the gene synthesis process is not thorough and comprehensive, and the synthesis efficiency is affected. The position of the reaction bin is inconvenient to adjust the angle, and the limitation in use is large.
The aim of the invention can be achieved by the following technical scheme:
a multi-angle upset reaction storehouse device for gene synthesis, includes first sleeve and mount pad, the mount pad rotates the top one end of installing at the loading box.
The one end rotation of mount pad is installed first sleeve, the driving gear is installed along the circumferencial direction to first telescopic outside, and mount pad one end is rotated at first telescopic outside and is installed a plurality of carrier bar, driven gear is installed along the circumferencial direction to the outside of carrier bar, driven gear is connected with the driving gear meshing, and the outside of carrier bar still overlaps and be equipped with detachable balancing weight, first sleeve is keeping away from the one end parallel connection of mount pad and has annular joint board, be connected with the first hydraulic column that a plurality of level set up between the tip edge of joint board and the first telescopic edge, joint board is kept away from first telescopic one end edge connection and is had the limiting plate, the inboard of limiting plate is connected with mobilizable baffle, the one end movable mounting that the limiting plate was kept away from to the baffle has a plurality of ball.
Preferably, the bearing seat is installed to the top other end of loading box, first motor is installed at the top of bearing seat, first motor passes through the supporting seat and is connected with the bearing seat, the output of first motor extends into the inside vertical fourth hydraulic stem that is connected with of bearing seat, the bottom of fourth hydraulic stem is connected with drive bevel gear, the inside of bearing seat rotates along the horizontal direction and installs the second sleeve, the both ends of second sleeve all stretch out from the inside both sides of bearing seat, driven bevel gear is installed along the circumferencial direction to the outside of second sleeve, driven bevel gear can be connected with the drive bevel gear meshing.
Preferably, one end of the bearing rod penetrates through the mounting seat to be connected with the mounting rod, one end of the first sleeve is connected with the output end of the second motor, and the second motor is mounted at the other end of the mounting seat.
Preferably, the limiting plate is connected with the joint plate through a connecting rod, and a second hydraulic column is connected between the baffle plate and the limiting plate.
Preferably, the balancing weight comprises a plurality of annular blocks, the annular blocks are sleeved outside the bearing rod, one end of the bearing rod is connected with the fixing nut in a threaded manner, and the balancing weight is arranged between the fixing nut and the driven gear.
Preferably, the fixing mechanism is installed in the first sleeve and the second sleeve, the fixing mechanism comprises a plurality of arc plates, third hydraulic columns are connected between the inner walls of the first sleeve and the second sleeve and the arc plates, and anti-slip pads are connected to the inner walls of the arc plates.
Preferably, the junction of the top of loading box and mount pad is provided with the mounting groove, and the both sides of mounting groove all are connected with the dwang, and the mount pad is installed in the inside of mounting groove, and the dwang of both sides all with the side fixed connection of mount pad.
Preferably, the specific steps of using the reaction chamber device include:
step one: the gene synthesis reaction bin is arranged in the first sleeve, one end of the reaction bin penetrates through the inside of the joint plate and enters the inside of the first sleeve, and a plurality of arc plates in the first sleeve are abutted with the outside of the reaction bin under the drive of the third hydraulic column to fix the reaction bin; the second hydraulic column on the side surface of the limiting plate drives the baffle to be in butt joint with the outside of the reaction bin, so that the reaction bin is further fixed; the balls at the end part of the baffle plate are contacted with the outer wall of the reaction bin, so that the friction force is reduced;
step two: in operation, the second motor drives the first sleeve to rotate so as to drive the reaction bin to rotate, and meanwhile, the driving gear drives the driven gears to rotate so as to drive the bearing rod to rotate; the balancing weight rotates along with the rotation of the bearing rod; before use, the number of annular blocks in each balancing weight is regulated, the weights among the balancing weights are different, the stress of the installation seat in each direction in rotation is unbalanced, and the reaction bin is rocked in rotation;
step three: one end of the reaction bin can be arranged in the second sleeve while working, and the other end of the reaction bin is arranged in the first sleeve, so that the reaction bin with a large length can be rotated for use; if the reaction bin is required to be driven to rotate by the second sleeve in the working process, the fourth hydraulic column moves downwards, the driving bevel gear is contacted with the driven bevel gear, and the first motor drives the driven bevel gear and the second sleeve to rotate through the driving bevel gear.
The invention has the beneficial effects that:
1. through at first telescopic internally mounted fixed establishment for can install the gene synthesis reaction storehouse in first telescopic inside in the work, the one end in reaction storehouse passes from the joint board inside and enters into first telescopic inside, the inside a plurality of arc of first sleeve is fixed with the outside butt in reaction storehouse under the drive of third hydraulic pressure post, makes the reaction storehouse more stable at first telescopic internally mounted, is difficult for rocking in the use and drops. And the second hydraulic column on the side surface of the limiting plate drives the baffle to be in butt joint with the outside of the reaction bin, so that the reaction bin can be further fixed. And a plurality of balls at the end part of the baffle plate are contacted with the outer wall of the reaction bin, so that the friction force can be reduced, the abrasion to the reaction bin is avoided, and the reaction bin is not easy to damage. The first sleeve and the baffle can adapt to the reaction chambers with different diameters, so that the reaction chambers are convenient and quick to install and detach.
2. Through the balancing weight of installation adjustable weight for in the work, the second motor can drive first sleeve rotation, and then drives the reaction storehouse and rotate, and the driving gear can drive a plurality of driven gear rotation simultaneously, and then drives the carrier bar and rotate. The balancing weight rotates along with the rotation of the bearing rod. Before use, adjust the quantity of annular piece in each balancing weight for the weight is different between each balancing weight, and then makes the rotation of different weight balancing weights can make the atress of mount pad all directions unbalanced, and then can obtain rocking in making the reaction storehouse rotate, can overturn different angles. Meanwhile, the rotating rod can drive the mounting seat to rotate, so that the overturning angle of the reaction bin is changed, the range of the angle change is enlarged, and different working requirements can be met.
3. In operation, one end of the reaction bin can be arranged in the second sleeve according to the working requirement, and the other end of the reaction bin is arranged in the first sleeve, so that the reaction bin with larger length can be rotated for use. If the reaction bin is required to be driven to rotate by the second sleeve in the working process, the fourth hydraulic column moves downwards, so that the driving bevel gear can be in contact with the driven bevel gear, and the first motor can drive the driven bevel gear and the second sleeve to rotate through the driving bevel gear. The reaction bin is convenient to rotate, and can adapt to different working requirements.
Drawings
The present invention is further described below with reference to the accompanying drawings for the convenience of understanding by those skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of the present invention.
Fig. 2 is a schematic view of the isometric structure of fig. 1 according to the present invention.
Fig. 3 is a schematic diagram of a limiting plate structure according to the present invention.
Fig. 4 is a schematic structural view of a second sleeve according to the present invention.
Fig. 5 is a schematic view of a supporting seat structure according to the present invention.
Fig. 6 is a schematic view of a second sleeve and bearing seat mounting structure according to the present invention.
Fig. 7 is a schematic view of the top structure of the loading box of the present invention.
In the figure: 1. a loading box; 2. a first motor; 3. balancing weight; 4. a driven gear; 5. a mounting rod; 6. a second motor; 7. a mounting base; 8. a first sleeve; 9. a first hydraulic column; 10. a limiting plate; 11. a second sleeve; 12. a connecting rod; 13. a joint plate; 14. a fixing nut; 15. a carrier bar; 16. a drive gear; 17. a second hydraulic column; 18. a baffle; 19. a ball; 20. an arc-shaped plate; 21. an anti-slip pad; 22. a third hydraulic column; 23. a drive bevel gear; 24. a fourth hydraulic column; 25. a support base; 26. a passive bevel gear; 27. a bearing seat; 28. a rotating lever; 29. and a mounting groove.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in connection with the embodiments, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1 to 7, a multi-angle overturning reaction chamber device for gene synthesis comprises a first sleeve 8 and a mounting seat 7, wherein the mounting seat 7 is rotatably arranged at one end of the top of a loading box 1;
the one end rotation of mount pad 7 is installed first sleeve 8, the driving gear 16 is installed along the circumferencial direction in the outside of first sleeve 8, and the one end rotation of mount pad 7 is installed a plurality of carrier bar 15 in the outside of first sleeve 8, the driven gear 4 is installed along the circumferencial direction in the outside of carrier bar 15, driven gear 4 is connected with driving gear 16 meshing, and the outside of carrier bar 15 still overlaps and is equipped with detachable balancing weight 3, first sleeve 8 is connected with annular joint board 13 in the one end parallel connection that keeps away from mount pad 7, be connected with the first hydraulic column 9 that a plurality of level set up between the tip edge of joint board 13 and the edge of first sleeve 8, the one end edge connection that joint board 13 kept away from first sleeve 8 has limiting plate 10, the inboard of limiting plate 10 is connected with mobilizable baffle 18, the one end movable mounting that limiting plate 10 was kept away from to baffle 18 has a plurality of ball 19.
As a technical optimization scheme of the invention, a bearing seat 27 is arranged at the other end of the top of the loading box 1, a first motor 2 is arranged at the top of the bearing seat 27, the first motor 2 is connected with the bearing seat 27 through a supporting seat 25, the output end of the first motor 2 extends into the bearing seat 27 and is vertically connected with a fourth hydraulic column 24, the bottom of the fourth hydraulic column 24 is connected with a drive bevel gear 23, a second sleeve 11 is rotatably arranged in the bearing seat 27 along the horizontal direction, both ends of the second sleeve 11 extend out of both sides of the interior of the bearing seat 27, a driven bevel gear 26 is arranged outside the second sleeve 11 along the circumferential direction, the driven bevel gear 26 can be meshed with the drive bevel gear 23, if the second sleeve 11 is required to drive a reaction bin to rotate in operation, the fourth hydraulic column 24 moves downwards, the drive bevel gear 23 can be contacted with the driven bevel gear 26, and the first motor 2 can drive the driven bevel gear 26 and the second sleeve 11 to rotate through the drive bevel gear 23. The reaction bin is convenient to rotate, and can adapt to different working requirements.
As a technical optimization scheme of the invention, one end of the bearing rod 15 penetrates through the mounting seat 7 to be connected with the mounting rod 5, one end of the first sleeve 8 is connected with the output end of the second motor 6, the second motor 6 is mounted at the other end of the mounting seat 7, and the second motor 6 can drive the first sleeve 8 to rotate.
As a technical optimization scheme of the invention, the limiting plate 10 is connected with the joint plate 13 through the connecting rod 12, a second hydraulic column 17 is connected between the baffle 18 and the limiting plate 10, and the second hydraulic column 17 can drive the baffle 18 to move so as to adapt to reaction bins with different sizes.
As a technical optimization scheme of the invention, the balancing weight 3 is composed of a plurality of annular blocks, the annular blocks are sleeved outside the bearing rod 15, one end of the bearing rod 15 is in threaded connection with the fixing nut 14, the balancing weight 3 is arranged between the fixing nut 14 and the driven gear 4, and the fixing nut 14 can be detached from the outside of the bearing rod 15, so that the weight of the balancing weight 3 can be conveniently adjusted.
As a technical optimization scheme of the invention, the interiors of the first sleeve 8 and the second sleeve 11 are respectively provided with a fixing mechanism, the fixing mechanism comprises a plurality of arc plates 20, third hydraulic columns 22 are respectively connected between the inner walls of the first sleeve 8 and the second sleeve 11 and the arc plates 20, the inner walls of the arc plates 20 are connected with anti-slip pads 21, and the arc plates 20 can freely move and adapt to reaction bins with different diameters.
As a technical optimization scheme of the invention, a mounting groove 29 is formed at the joint of the top of the loading box 1 and the mounting seat 7, the two sides of the mounting groove 29 are connected with rotating rods 28, the mounting seat 7 is mounted in the mounting groove 29, the rotating rods 28 on the two sides are fixedly connected with the side surfaces of the mounting seat 7, and the rotating rods 28 can drive the mounting seat 7 to rotate in the mounting groove 29.
As a technical optimization scheme of the invention, the reaction chamber device comprises the following specific steps:
step one: the gene synthesis reaction bin is arranged in the first sleeve 8, one end of the reaction bin penetrates through the inside of the joint plate 13 and enters the inside of the first sleeve 8, and a plurality of arc plates 20 in the first sleeve 8 are abutted with the outside of the reaction bin under the drive of a third hydraulic column 22 to fix the reaction bin; the second hydraulic column 17 on the side surface of the limiting plate 10 drives the baffle 18 to be abutted with the outside of the reaction bin, so that the reaction bin is further fixed; the balls 19 at the end part of the baffle 18 are contacted with the outer wall of the reaction bin, so that the friction force is reduced;
step two: in operation, the second motor 6 drives the first sleeve 8 to rotate so as to drive the reaction bin to rotate, and meanwhile, the driving gear 16 drives the driven gears 4 to rotate so as to drive the bearing rod 15 to rotate; the balancing weight 3 rotates along with the rotation of the bearing rod 15; before use, the number of annular blocks in each balancing weight 3 is regulated, the weights among the balancing weights 3 are different, the stress of the installation seat 7 in each direction in rotation is unbalanced, and the reaction bin is rocked in rotation;
step three: one end of the reaction bin can be arranged in the second sleeve 11 in the working process, and the other end of the reaction bin is arranged in the first sleeve 8, so that the reaction bin with a large length is rotated; if the second sleeve 11 is required to drive the reaction chamber to rotate during operation, the fourth hydraulic column 24 moves downwards, the drive bevel gear 23 contacts with the driven bevel gear 26, and the first motor 2 drives the driven bevel gear 26 and the second sleeve 11 to rotate through the drive bevel gear 23.
When the invention is used, the gene synthesis reaction bin is arranged in the first sleeve 8, one end of the reaction bin passes through the joint plate 13 and enters the first sleeve 8, the arc plates 20 in the first sleeve 8 are driven by the third hydraulic column 22 to be abutted with the outside of the reaction bin, and the reaction bin is fixed, so that the reaction bin is more stably arranged in the first sleeve 8 and is not easy to shake and fall off in use. And the second hydraulic column 17 on the side surface of the limiting plate 10 drives the baffle 18 to be in butt joint with the outside of the reaction bin, so that the reaction bin can be further fixed. And a plurality of balls 19 at the end part of the baffle 18 are contacted with the outer wall of the reaction chamber, so that friction force can be reduced, abrasion to the reaction chamber is avoided, and the reaction chamber is not easy to damage. The first sleeve 8 and the baffle 18 can adapt to reaction chambers with different diameters, so that the reaction chambers are convenient and quick to install and detach.
In operation, the second motor 6 can drive the first sleeve 8 to rotate, so as to drive the reaction bin to rotate, and meanwhile, the driving gear 16 can drive the driven gears 4 to rotate, so as to drive the bearing rod 15 to rotate. The weight 3 rotates with the rotation of the carrier bar 15. Before use, adjust the quantity of annular piece in each balancing weight 3 for the weight is different between each balancing weight 3, and then makes the rotation of different weight balancing weights can make the atress of mount pad 7 each direction unbalanced, and then can obtain rocking in making the reaction storehouse rotate, can overturn different angles. Meanwhile, the rotating rod 28 can drive the mounting seat 7 to rotate, so that the overturning angle of the reaction bin is changed, the range of the angle change is enlarged, and different working requirements can be met.
In operation, one end of the reaction bin can be arranged in the second sleeve 11 according to the working requirement, and the other end of the reaction bin is arranged in the first sleeve 8, so that the reaction bin with larger length can be rotated for use. If the second sleeve 11 is required to drive the reaction chamber to rotate during operation, the fourth hydraulic column 24 moves downwards, so that the driving bevel gear 23 can be in contact with the driven bevel gear 26, and the first motor 2 can drive the driven bevel gear 26 and the second sleeve 11 to rotate through the driving bevel gear 23. The reaction bin is convenient to rotate, and can adapt to different working requirements.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.

Claims (6)

1. The multi-angle overturning reaction bin device for gene synthesis is characterized by comprising a first sleeve (8) and a mounting seat (7), wherein the mounting seat (7) is rotatably arranged at one end of the top of a loading box (1);
the novel hydraulic lifting device is characterized in that a first sleeve (8) is rotatably arranged at one end of the mounting seat (7), a driving gear (16) is arranged at the outer part of the first sleeve (8) along the circumferential direction, a plurality of bearing rods (15) are rotatably arranged at one end of the mounting seat (7) at the outer part of the first sleeve (8), a driven gear (4) is arranged at the outer part of the bearing rods (15) along the circumferential direction, the driven gear (4) is meshed with the driving gear (16), a detachable balancing weight (3) is sleeved outside the bearing rods (15), an annular joint plate (13) is connected in parallel at one end far away from the mounting seat (7), a plurality of first hydraulic columns (9) which are horizontally arranged are connected between the end edges of the joint plate (13) and the edges of the first sleeve (8), a limit plate (10) is connected at one end edge of the joint plate (13) far away from the first sleeve (8), a movable baffle plate (18) is connected at the inner side of the limit plate (10), and a plurality of balls (19) are movably arranged at one end of the baffle plate (18) far away from the limit plate (10);
one end of the bearing rod (15) penetrates through the mounting seat (7) to be connected with the mounting rod (5), one end of the first sleeve (8) is connected with the output end of the second motor (6), and the second motor (6) is mounted at the other end of the mounting seat (7);
the top of loading case (1) is provided with mounting groove (29) with the junction of mount pad (7), and the both sides of mounting groove (29) all are connected with dwang (28), and inside at mounting groove (29) is installed in mount pad (7), and the side fixed connection of dwang (28) of both sides and mount pad (7).
2. The multi-angle overturning reaction bin device for gene synthesis according to claim 1, wherein a bearing seat (27) is arranged at the other end of the top of the loading box (1), a first motor (2) is arranged at the top of the bearing seat (27), the first motor (2) is connected with the bearing seat (27) through a supporting seat (25), the output end of the first motor (2) extends into the bearing seat (27) and is vertically connected with a fourth hydraulic column (24), the bottom of the fourth hydraulic column (24) is connected with a driving bevel gear (23), a second sleeve (11) is rotatably arranged in the bearing seat (27) along the horizontal direction, two ends of the second sleeve (11) extend out from two sides of the inside of the bearing seat (27), a driven bevel gear (26) is arranged at the outer part of the second sleeve (11) along the circumferential direction, and the driven bevel gear (26) can be meshed with the driving bevel gear (23).
3. The multi-angle overturning reaction chamber device for gene synthesis according to claim 1, wherein the limiting plate (10) is connected with the joint plate (13) through a connecting rod (12), and a second hydraulic column (17) is connected between the baffle plate (18) and the limiting plate (10).
4. The multi-angle overturning reaction bin device for gene synthesis according to claim 1, wherein the balancing weight (3) is composed of a plurality of annular blocks, the annular blocks are sleeved outside the bearing rod (15), one end of the bearing rod (15) is connected with the fixing nut (14) in a threaded mode, and the balancing weight (3) is arranged between the fixing nut (14) and the driven gear (4).
5. The multi-angle overturning reaction chamber device for gene synthesis according to claim 1, wherein the first sleeve (8) and the second sleeve (11) are internally provided with fixing mechanisms, each fixing mechanism comprises a plurality of arc plates (20), third hydraulic columns (22) are connected between the inner walls of the first sleeve (8) and the second sleeve (11) and the arc plates (20), and anti-slip pads (21) are connected to the inner walls of the arc plates (20).
6. The multi-angle flip cartridge device for gene synthesis according to claim 1, wherein the specific steps of using the cartridge device include:
step one: the gene synthesis reaction bin is arranged in the first sleeve (8), one end of the reaction bin penetrates through the inside of the joint plate (13) and enters the inside of the first sleeve (8), and a plurality of arc plates (20) in the first sleeve (8) are abutted with the outside of the reaction bin under the drive of a third hydraulic column (22) to fix the reaction bin; the second hydraulic column (17) on the side surface of the limiting plate (10) drives the baffle plate (18) to be in butt joint with the outside of the reaction bin, so that the reaction bin is further fixed; and a plurality of balls (19) at the end part of the baffle plate (18) are contacted with the outer wall of the reaction bin, so that the friction force is reduced;
step two: in operation, the second motor (6) drives the first sleeve (8) to rotate so as to drive the reaction bin to rotate, and meanwhile, the driving gear (16) drives the driven gears (4) to rotate so as to drive the bearing rod (15) to rotate; the balancing weight (3) rotates along with the rotation of the bearing rod (15); before use, the number of annular blocks in each balancing weight (3) is regulated, the weights of the balancing weights (3) are different, the stress of the installation seat (7) in each direction in rotation is unbalanced, and the reaction bin is rocked in rotation;
step three: one end of the reaction bin can be arranged in the second sleeve (11) in the working process, and the other end of the reaction bin is arranged in the first sleeve (8), so that the reaction bin with a large length is rotated; if the second sleeve (11) is required to drive the reaction bin to rotate in the working process, the fourth hydraulic column (24) moves downwards, the driving bevel gear (23) is in contact with the driven bevel gear (26), and the first motor (2) drives the driven bevel gear (26) and the second sleeve (11) to rotate through the driving bevel gear (23).
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