CN111567557A - Initiator for improving cold resistance of tobacco seeds and initiation method - Google Patents
Initiator for improving cold resistance of tobacco seeds and initiation method Download PDFInfo
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- CN111567557A CN111567557A CN202010612897.9A CN202010612897A CN111567557A CN 111567557 A CN111567557 A CN 111567557A CN 202010612897 A CN202010612897 A CN 202010612897A CN 111567557 A CN111567557 A CN 111567557A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention relates to the technical field of seed treatment, in particular to an initiator and a method for improving cold resistance of tobacco seeds. According to the method, a sodium selenite solution is used as an initiator, the tobacco naked seeds are subjected to initiation treatment for 24-48 hours under the dark condition at the temperature of 25 ℃, the ratio of the tobacco naked seeds to the initiator is 1kg: 5-15L, and the initiator solution submerges 5-10 cm of the seeds; and (3) washing the tobacco naked seeds subjected to initiation treatment for 3-4 times by using clear water, and placing the tobacco naked seeds in an indoor ventilation place to be dried back to the original weight of the tobacco naked seeds. Can be beneficial to enhancing the cold resistance of tobacco seeds, improving the germination rate of the tobacco seeds at low temperature, accelerating the germination speed of the tobacco seeds, improving the seedling emergence regularity of the tobacco seeds, further avoiding economic loss caused by low temperature and increasing the yield.
Description
Technical Field
The invention relates to the technical field of seed treatment, in particular to an initiator and an initiating method for improving cold resistance of tobacco seeds.
Background
Tobacco (A)Nicotiana tabacumL.) is one of the important economic crops in China, and the yield of the crops accounts for 39.6 percent of the global tobacco yield. Tobacco planting in China is widely distributed in 22 provinces, nearly 1500 ten thousand population groups participate in tobacco planting and selling, and healthy development of tobacco industry plays a vital role in national rural economic development and farmer income. Tobacco seeds are the basis for ensuring high-quality and stable yield of tobacco leaves, and the early spring low-temperature harm is a common problem in tobacco regions in the south of China, namely, the low temperature can influence the germination of the tobacco seeds, the growth of tobacco seedlings, flower bud differentiation, photosynthesis and the like, so that the yield and the quality of the tobacco leaves are reduced.
Disclosure of Invention
Aiming at the technical problems and defects that the yield and the quality of tobacco leaves are reduced due to the low germination rate of tobacco seeds at low temperature, namely the cold resistance of the tobacco seeds is poor at present.
The invention is realized by the following technical scheme:
an initiator for improving the cold resistance of tobacco seeds comprises the active ingredient of sodium selenite solution.
Further, the selenium content of the sodium selenite solution is 0.5-2.0 mg/L.
Further, the selenium content of the sodium selenite solution is preferably 1.0 mg/L.
In order to achieve the above object, the present invention further provides a method for improving the cold resistance of tobacco seeds, wherein the method specifically comprises the following steps:
weighing tobacco naked seeds, adopting a sodium selenite solution as an initiator, and carrying out initiation treatment on the tobacco naked seeds for 24-48 h under the dark condition at the temperature of 25 ℃, wherein the ratio of the tobacco naked seeds to the initiator is 1kg: 5-15L, and the solution of the initiator submerges the seeds for 5-10 cm;
and (3) washing the tobacco naked seeds subjected to initiation treatment for 3-4 times by using clear water, and placing the tobacco naked seeds in an indoor ventilation place to be dried back to the original weight of the tobacco naked seeds.
Further, the selenium content of the sodium selenite solution is 0.5-2.0 mg/L.
Further, the selenium content of the sodium selenite solution is preferably 1.0 mg/L.
Further, the priming treatment of the tobacco naked seeds under the dark condition at the temperature of 25 ℃ is 36 hours.
Further, the ratio of the tobacco naked seeds to the initiator is 1kg to 10L.
In order to achieve the purpose, the invention also provides an application of the initiator, and the initiator is applied to the initiation treatment of tobacco seeds.
Compared with the prior art, the invention has the following beneficial effects:
according to the initiator and the method for improving the cold resistance of the tobacco seeds, the cold resistance of the tobacco seeds is favorably enhanced, the germination rate of the tobacco seeds at low temperature is improved, the germination speed of the tobacco seeds is accelerated, the seedling emergence regularity of the tobacco seeds is improved, the economic loss caused by low temperature is avoided, and the yield is increased.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic flow chart of a method for improving cold resistance of tobacco seeds according to the present invention;
the objects, features and advantages of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
For better understanding of the objects, aspects and advantages of the present invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings, and other advantages and capabilities of the present invention will become apparent to those skilled in the art from the description.
The invention is capable of other and different embodiments and its several details are capable of modification in various other respects, all without departing from the spirit and scope of the present invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components, the movement situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. Secondly, the technical solutions in the embodiments can be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not be within the protection scope of the present invention.
The invention relates to an initiator for improving the cold resistance of tobacco seeds and an initiation method.
Seed priming is one of the seed sowing pretreatment technologies commonly used in agriculture, and can improve the seed vigor, ensure that seeds quickly and regularly germinate and emerge, improve the stress resistance and the like. Selenium is an essential element for humans and animals. Selenium with proper concentration has various beneficial physiological effects on plant growth, can promote seed germination and improve stress resistance of seeds and seedlings, provides an initiator and an initiation method for improving cold resistance of seeds by using selenium element, has higher agricultural production utilization value, and has important significance for improving yield and quality of tobacco. The invention is realized by the following technical scheme:
an initiator for improving the cold resistance of tobacco seeds comprises the active ingredient of sodium selenite solution.
Specifically, the selenium content of the sodium selenite solution is 0.5-2.0 mg/L. Preferably, the selenium content of the sodium selenite solution is 1.0 mg/L.
That is, selenium with proper concentration has various beneficial physiological effects on plant growth, has the effects of eliminating excessive free radicals and relieving the damage of stress on plants, and can promote seed germination and improve the stress resistance of seeds and seedlings. In addition, selenium with proper concentration also has the functions of improving the activity of various enzymes and the chlorophyll content, promoting root system development and the like.
In order to achieve the above object, the present invention further provides a method for improving the cold resistance of tobacco seeds, as shown in fig. 1, the method specifically comprises the following steps:
s1, weighing tobacco naked seeds, adopting a sodium selenite solution as an initiator, and carrying out initiation treatment on the tobacco naked seeds for 24-48 h under the dark condition at 25 ℃, wherein the ratio of the tobacco naked seeds to the initiator is 1kg: 5-15L, and the sodium selenite solution submerges the seeds for 5-10 cm;
and S2, washing the tobacco naked seeds subjected to initiation treatment for 3-4 times by using clean water, and putting the tobacco naked seeds in an indoor ventilation place to be dried back to the original weight of the tobacco naked seeds.
In other words, the method comprises at least the following steps:
firstly, taking weighed tobacco seeds, and carrying out initiation treatment on the weighed tobacco seeds for 24-48 h by using a sodium selenite solution with the mass fraction of selenium of 0.5-2.0 mg/L under the dark condition at the temperature of 25 ℃, wherein the ratio of the seeds to an initiator is 1kg to 5-15L, and the initiation solution submerges the seeds for 5-10 cm;
and step two, taking the seeds initiated in the step one, washing the seeds for 3-4 times by using clear water, and drying the seeds at a room-temperature ventilated place to the original weight of the seeds.
Preferably, in the step one, the mass concentration of selenium in the initiator is preferably 1.0 mg/L.
Preferably, in the first step, the time for priming the tobacco seeds in the sodium selenite solution is preferably 36 h.
Furthermore, the ratio of the tobacco naked seeds to the initiator is 1kg to 10L.
The method can be used for pretreating tobacco seeds before field sowing, has low concentration of the initiator, low initiation cost and good effect, is beneficial to enhancing the cold resistance of the tobacco seeds, accelerating the germination speed of the seeds, improving the seedling emergence regularity of the seeds and avoiding economic loss caused by low temperature.
In order to achieve the purpose, the invention also provides an application of the initiator, and the initiator is applied to the initiation treatment of tobacco seeds.
Example (b):
test materials:
A. and B, tobacco seed batches of two varieties are provided by tobacco seed Limited liability company in Yuxi Yunnan.
The experimental method comprises the following steps:
initiating treatment of tobacco seeds: respectively carrying out initiation treatment on weighed tobacco seeds in sodium selenite solutions with different concentrations for 36h at 25 ℃ in the dark, wherein the ratio of the seeds to the initiator is 1kg to 10L, and the initiation solution is 5cm above the seeds. And (4) after the initiation is finished, washing the seeds for 3-4 times by using clear water, and drying the seeds at a room-temperature ventilated place until the seeds are dried to the original weight.
A total of 6 treatments were set, as follows:
process 1 (CK 1): non-primed dry seed;
process 2 (CK 2): water priming seeds;
and (3) treatment: initiating seeds by using a sodium selenite solution with the selenium mass concentration of 0.5 mg/L;
and (4) treatment: initiating seeds by using a sodium selenite solution with the selenium mass concentration of 1.0 mg/L;
and (4) treatment 5: initiating seeds by using a sodium selenite solution with the selenium mass concentration of 1.5 mg/L;
and (6) treatment: the sodium selenite solution with the selenium mass concentration of 2.0mg/L initiates seeds.
And (3) low-temperature germination test:
culturing for 2d under the conditions of illumination at 30 ℃ for 8h/20 ℃ in the dark for 16h, moving to the low temperature of 11 ℃ when the seeds are exposed to the white, culturing for 7d under the conditions of illumination at 8 h/dark for 16h, and culturing for 7d under the conditions of illumination at 30 ℃ for 8h/20 ℃ in the dark for 16 h. The number of germinated seeds was counted daily, the germination vigour (GE) was calculated on day 7, and the germination rate (GP), Germination Index (GI) and Vigor Index (VI) were calculated on day 16. The Root Length (RL) and plant height (SH) were measured using a ruler, and the dry weight of roots and seedlings (RDW and SDW) were determined after drying at 80 ℃ for 24 hours.
In the embodiment of the invention:
germination potential (%) = (number of germinated seeds/number of examined seeds in 7 d) × 100%
Germination percentage (%) = (number of germinated seeds/number of examined seeds in 16 d) × 100%
Germination index =
(ii) a Wherein:
in order to sprout the seeds one day by one day,
for sending out correspondinglyThe number of shoots.
Viability index = germination index x dry seedling weight.
Statistical analysis: statistical analysis of data using Excel 2016 software, single-factor variance (ANOVA) analysis at 95% level using SAS software, multiple comparisons and significance of differences test using LSD test (see
=0.05）。
And (4) analyzing results:
after the initiation of 0.5-2.0 mg/L sodium selenite, the seed vigor of the two tobacco varieties at low temperature is remarkably improved, and the germination speed is accelerated (tables 1 and 2).
Compared with non-initiated dry seeds, the germination potential of seeds of the variety A treated by sodium selenite initiation is improved by 23-37.7%, the germination rate is improved by 3.7-7.2%, the average germination time is shortened by 0.6-0.8d, the length of 16d seedlings is increased by about 0.01-0.26cm, and the length of the seedlings treated by 0.5mg/L is obviously increased; the germination vigor of seeds of the B variety is improved by 48.5-60.7%, the germination rate is improved by 0.2-2.2%, the average germination time is shortened by 1.1-1.3 days, the length of 16d seedlings is increased by 0.18-0.51cm, and the germination rate of the seeds except for the 2.0mg/L treatment in the four indexes reaches an obvious level. The results of several indexes are integrated, and the sodium selenite with the selenium mass concentration of 1.0mg/L is shown to have the best initiating effect, so that the germination vigor, the germination rate, the germination index and the vitality index of A, B two varieties of tobacco seeds are obviously improved.
TABLE 1 Effect of sodium selenite solution initiation on Low temperature Germination of seeds of tobacco variety A
Wherein: letter indicates the comparison of significance of difference between different treatment groups (ii)
=0.05, LSD). CK1 represents unprimed dry seed and CK2 represents water-primed seed.
TABLE 2 Effect of sodium selenite initiation on Low temperature Germination of seeds of tobacco variety B
Wherein: letter indicates the comparison of significance of difference between different treatment groups (ii)
=0.05, LSD). CK1 represents unprimed dry seed and CK2 represents water-primed seed.
The result analysis of the embodiment shows that the initiator and the method for improving the cold resistance of the tobacco seeds are beneficial to enhancing the cold resistance of the tobacco seeds, improving the germination rate of the tobacco seeds at low temperature, accelerating the germination speed of the tobacco seeds, improving the seedling emergence regularity of the tobacco seeds, further avoiding economic loss caused by low temperature and increasing yield. But also can improve the vitality of the seeds, lead the seeds to germinate quickly and regularly, improve the stress resistance and the like. Under the control condition, the tobacco seeds are promoted to slowly absorb water without sprouting, and the internal enzyme of the tobacco seeds is activated, and the sprouting promoting metabolites are increased.
In other words, the sodium selenite in the scheme of the invention is used as the initiator to treat the tobacco seeds so as to achieve the purposes of improving the germination rate of the tobacco seeds and the quality of seedlings under the low-temperature condition, and the initiator has lower required concentration, low initiation cost and better effect, is beneficial to enhancing the cold resistance of the tobacco seeds, accelerating the germination speed of the seeds, improving the seedling emergence regularity of the seeds and avoiding economic loss caused by low temperature.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (9)
1. An initiator for improving the cold resistance of tobacco seeds is characterized in that: the effective component of the initiator is sodium selenite.
2. The initiator for improving the cold resistance of tobacco seeds as claimed in claim 1, wherein: the selenium content of the sodium selenite solution is 0.5-2.0 mg/L.
3. The initiator for improving the cold resistance of tobacco seeds as claimed in claim 2, wherein: the selenium content of the sodium selenite solution is 1.0 mg/L.
4. A method for improving the cold resistance of tobacco seeds is characterized by comprising the following steps:
weighing tobacco naked seeds, adopting a sodium selenite solution as an initiator, and carrying out initiation treatment on the tobacco naked seeds for 24-48 h under the dark condition at the temperature of 25 ℃, wherein the ratio of the tobacco naked seeds to the initiator is 1kg: 5-15L, and the sodium selenite solution submerges the seeds for 5-10 cm;
and (3) washing the tobacco naked seeds subjected to initiation treatment for 3-4 times by using clear water, and placing the tobacco naked seeds in an indoor ventilation place to be dried back to the original weight of the tobacco naked seeds.
5. The method for improving cold resistance of tobacco seeds as claimed in claim 4, wherein the selenium content of the sodium selenite solution is 0.5-2.0 mg/L.
6. The method for improving cold resistance of tobacco seeds as claimed in claim 5, wherein the selenium content of the sodium selenite solution is 1.0 mg/L.
7. The method for improving the cold resistance of tobacco seeds as claimed in claim 4, wherein the priming treatment of the naked tobacco seeds at 25 ℃ in the dark is carried out for 36 h.
8. The method of claim 4, wherein the ratio of the naked tobacco seed to the initiator is 1kg: 10L.
9. Use of an initiator according to claim 1 or 2 or 3, wherein the initiator is used in the initiation treatment of tobacco seeds.
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Cited By (3)
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| CN113615341A (en) * | 2021-08-12 | 2021-11-09 | 玉溪中烟种子有限责任公司 | Method for improving activity and low-temperature stress resistance of tobacco seeds |
| CN113994974A (en) * | 2021-11-17 | 2022-02-01 | 湖南省烟草公司永州市公司 | Initiator for improving cold resistance of tobacco seeds, initiation method and application |
| CN114262243A (en) * | 2021-12-25 | 2022-04-01 | 西北农林科技大学 | Cucumber sub-low temperature resistant selenium-rich nutrient solution and preparation method and application thereof |
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| CN114262243A (en) * | 2021-12-25 | 2022-04-01 | 西北农林科技大学 | Cucumber sub-low temperature resistant selenium-rich nutrient solution and preparation method and application thereof |
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Application publication date: 20200825 |