CN111558347A - Gel preparation facilities for electrophoresis - Google Patents
Gel preparation facilities for electrophoresis Download PDFInfo
- Publication number
- CN111558347A CN111558347A CN202010178135.2A CN202010178135A CN111558347A CN 111558347 A CN111558347 A CN 111558347A CN 202010178135 A CN202010178135 A CN 202010178135A CN 111558347 A CN111558347 A CN 111558347A
- Authority
- CN
- China
- Prior art keywords
- rubber plate
- gel
- boundary line
- upper rubber
- cavity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims description 9
- 238000003466 welding Methods 0.000 claims description 9
- 230000007704 transition Effects 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 6
- 229910003460 diamond Inorganic materials 0.000 claims description 2
- 239000010432 diamond Substances 0.000 claims description 2
- 239000000499 gel Substances 0.000 description 54
- 239000003292 glue Substances 0.000 description 18
- 230000008569 process Effects 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 239000000084 colloidal system Substances 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 4
- 230000005012 migration Effects 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 3
- 241001391944 Commicarpus scandens Species 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 239000002808 molecular sieve Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/0052—Preparation of gels
- B01J13/0065—Preparation of gels containing an organic phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Electrochemistry (AREA)
- Electrostatic Separation (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The invention discloses a gel preparation device for electrophoresis, which comprises comb teeth, an upper rubber plate and a lower rubber plate, wherein the upper rubber plate and the lower rubber plate are mutually buckled to form a cavity with an opening at the top and capable of containing gel; the comb teeth comprise racks and comb bodies, the comb teeth are covered on the top opening of the cavity, and the racks face the inside of the cavity; a rectangular liquid leakage groove is arranged on the comb body along the length direction of the comb body, and a channel is arranged between the racks and is communicated with the liquid leakage groove; according to the comb, the liquid leakage groove is formed in the comb body, so that after the comb teeth are inserted into gel, the redundant gel is introduced into the liquid leakage groove, and deformation caused by expansion of the gel is avoided.
Description
Technical Field
The invention belongs to the technical field of gel electrophoresis instruments, and particularly relates to a gel preparation device for electrophoresis in polyacrylamide gel electrophoresis.
Background
Gel electrophoresis (English) or colloidal electrophoresis is a large class of techniques used by scientists to separate molecules of different physical properties (e.g., size, shape, isoelectric point, etc.). Polyacrylamide gel electrophoresis (abbreviated as page) is a common electrophoresis technique using polyacrylamide gel as a support medium, and is the most common technique in protein separation and identification. When the electrophoretic separation is carried out, the charged particles can carry out reversed-phase electrode migration under the influence of an external magnetic field, the movement of the particles is slowed down by the interference of a surrounding gel medium, and a molecular sieve is formed; under the combined influence of electric field force and molecular sieve, proteins in a detection sample show different migration rates, the molecular mass of the proteins can be estimated through the relative migration of the proteins in standard PAGE gels, and a single strip of the gel can be used as a standard for judging the purity of the proteins.
At present, most electrophoresis is carried out in a vertical electrophoresis tank of a glue making plate consisting of two glass plates, the glue making plate on the market at present basically has no fixing device or is fixed on two sides, and when the glue making plate is used for filling a front clamping plate, the two plates are not easy to be completely aligned, so that liquid leakage is easy to cause. Usually before the encapsulating, need add water in the cavity of the formation of two offset plates to verify its leakproofness, complex operation, and remaining water stain can influence the effect of electrophoresis, even set up buckle structure in prefabricated offset plate both sides at present, in order to accomplish the fixed of two offset plates, nevertheless when encapsulating splint, can cause the bottom weeping equally.
In addition, in the glue filling process, expansion of gel colloid can occur, the colloidal volume formed by the gel is more than the volume of the original solution, the whole gel process is that the glue solution with high concentration at the lower end is solidified first, therefore, the glue solution with low concentration and not solidified at the upper end can generate an extrusion process for the glue solution with low concentration and not solidified at the lower end, if the fit degree of the comb teeth and the opening at the upper end is poor, the expanded gel colloid is easy to extrude the comb teeth, so that the inclination change of the original positions of the comb teeth is caused, and the tooth holes formed on the gel are damaged; the degree of agreeing with of broach and upper end opening is better, then can cause the above-mentioned low concentration gel to be extruded (because of the gel compressibility ratio of low concentration is good than concentration), and after using gel to pull out the broach, the reservation tooth hole interval department of gel can shift up to cause the reduction of tooth hole volume.
Disclosure of Invention
In order to solve the technical problems, the invention provides a gel preparation device for electrophoresis in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, wherein a liquid leakage groove is arranged on a comb body, so that redundant gel is introduced into the liquid leakage groove after comb teeth are inserted into the gel, and deformation caused by expansion of the gel is avoided.
The technical scheme provided by the invention is as follows:
a gel preparation device for electrophoresis comprises a rubber plate and comb teeth, wherein the rubber plate comprises an upper rubber plate and a lower rubber plate which are mutually buckled to form a cavity with an opening at the top and capable of containing gel; the comb teeth comprise racks and comb bodies, the comb teeth are covered on the top opening of the cavity, and the racks face the inside of the cavity; a rectangular liquid leakage groove is formed in the comb body along the length direction of the comb body, a channel is arranged between the racks, and the channel is communicated with the liquid leakage groove.
Preferably, the bottom and two sides of the buckled upper rubber plate and lower rubber plate are fixedly connected, the lower end of the upper rubber plate or the lower rubber plate is provided with two or more open grooves, the open grooves are communicated with the inside of the cavity and used for connecting the electrode grooves and serving as the lower openings of the electrophoresis conducting openings, and removable sealing strips are arranged on the open grooves.
More preferably, the upper rubber plate and the lower rubber plate are fixed by ultrasonic welding.
More preferably, a plurality of limiting columns are arranged on two sides of the upper rubber plate or the lower rubber plate and used for limiting the thickness of the ultrasonic welding.
Preferably, two U-shaped structures are respectively arranged on two side edges of the upper rubber plate, the open ends of the two U-shaped structures are oppositely arranged, two rectangular bulges A are correspondingly arranged on the lower rubber plate, and the bulges A are buckled into a gap formed by the two U-shaped structures.
Preferably, a cuboid-shaped bulge B is arranged on the face, opposite to the liquid leakage groove, of the comb body along the length direction of the comb body, and the thickness of the bulge B is consistent with that of the adjacent rubber plate.
Preferably, a plurality of grooves are arranged on the inner side of the rubber plate on the same side with the liquid leakage groove along the length direction of the rubber plate, and the vertical height of the grooves on the rubber plate is larger than that of gel filled into the cavity and is lower than that of the tail ends of the tops of the comb teeth inserted into the cavity.
More preferably, the shape of the groove may be circular, diamond, square or rectangular.
Preferably, a boundary line is arranged along the length direction of the upper rubber plate from the bottom to the height of 2/3-4/5 on the side, opposite to the lower rubber plate, of the upper rubber plate, the thickness of the upper rubber plate below the boundary line is larger than that of the upper rubber plate above the boundary line, and the upper rubber plates with different thicknesses on the boundary line are in arc-shaped transition arrangement.
Preferably, a boundary line is arranged along the length direction of the lower rubber plate from the bottom to the height of 2/3-4/5 on the side, opposite to the upper rubber plate, of the lower rubber plate, the thickness of the lower rubber plate below the boundary line is larger than that of the lower rubber plate above the boundary line, and the lower rubber plates with different thicknesses on the boundary line are in arc-shaped transition arrangement.
More preferably, a boundary line is arranged on the upper rubber plate at a position opposite to the lower rubber plate and at the same height along the direction of the boundary line, the thickness of the upper rubber plate below the boundary line is greater than that of the upper rubber plate above the boundary line, and the upper rubber plates with different thicknesses at the boundary line are in arc-shaped transition arrangement.
Compared with the prior art, the invention has the following technical advantages:
(1) the gel preparation device for electrophoresis is fixed on three sides, only has an opening at the top, and the upper and lower rubber plates are combined more firmly, so that the sealing property of the whole device is improved, and meanwhile, the deformation of the upper and lower rubber plates caused by extrusion is reduced to the greatest extent, and a good electrophoresis environment is ensured;
(2) the upper and lower rubber plates are welded by ultrasonic waves, high-frequency vibration waves are transmitted to the surfaces of two objects to be welded, and the surfaces of the two objects are mutually rubbed under the condition of pressurization to form fusion between molecular layers. Compared with the traditional welding process, the welding process is stable, the welding strength is high, the prepared rubber plate has good sealing performance, no liquid leakage, cleanness and no pollution;
(3) the lower end of the upper rubber plate or the lower rubber plate is provided with a plurality of open slots for current outlet, the open slots are communicated with the cavity between the upper rubber plate and the lower rubber plate, the rigidity of the whole device is increased, if only one open slot is arranged, the clip of the electrophoresis tank is fixed on the upper rubber plate or the lower rubber plate around the open slot, the gel preparation device for electrophoresis is easy to break, two or more open slots are arranged, the open slots are spaced, the stress is dispersed, and the upper rubber plate or the lower rubber plate is not easy to break; in a similar way, due to the dispersion of stress, the arrangement of the open slot can also effectively avoid the deformation problem of the prepared gel caused by stress extrusion in the transportation process;
(4) the comb body is provided with the liquid leakage groove, and partial gel liquid extruded due to the expansion of the gel volume is introduced through a channel reserved between the racks communicated with the liquid leakage groove, so that the deformation of a colloid in the gel preparation device due to the expansion is avoided; the gel can be effectively cut off after the comb teeth are pulled out in the later period, and the reserved tooth hole interval of the gel cannot move upwards, so that the volume of the tooth hole cannot be reduced;
(5) the comb body is provided with the cuboid-shaped protrusion B, after the comb teeth are inserted into a certain position in the cavity, the protrusion B is flush with the edge of the adjacent rubber plate, at the moment, the comb teeth cannot continuously extend into the cavity, and the comb teeth position can be determined;
(6) the invention is arranged on the rubber plate at the same side with the liquid leakage groove, and a plurality of grooves are arranged at the inner side of the rubber plate along the length direction of the rubber plate, and the grooves can also contain partial gel liquid extruded due to the expansion of the volume of the gel, and can also avoid the deformation of the gel in the gel preparation device due to the expansion;
(7) the upper and lower parts of the upper rubber plate and/or the lower rubber plate in the invention have different thicknesses, so that the upper half part of the formed cavity has large volume and the lower half part has small volume; the problem that the sample injector extends into the cavity to cause deformation of the rubber plate can be effectively avoided.
Drawings
FIG. 1 is a schematic front view of the upper glue plate of the present invention
FIG. 2 is a rear view of the upper glue plate of the present invention
FIG. 3 is a schematic perspective view of a lower rubber plate according to the present invention
FIG. 4 is a schematic front view of the comb teeth of the present invention
FIG. 5 is a rear view of the comb teeth of the present invention
FIG. 6 is a schematic front view of a lower rubber plate according to another embodiment of the present invention
FIG. 7 is a longitudinal sectional view of the present invention
Wherein: 1. comb teeth 2, an upper rubber plate 3, a lower rubber plate 4, an open slot 5, a limiting column 6, a protrusion 7 with a U-shaped structure, a protrusion A8, a rack 9, a comb body 10, a liquid leakage groove 11, a channel 12, a protrusion B13 and a groove.
Detailed Description
The present invention will be further described with reference to the following detailed description and accompanying drawings.
As shown in fig. 1 to 5, the invention discloses a gel preparation device for electrophoresis, which comprises comb teeth 1, an upper rubber plate 2 and a lower rubber plate 3, wherein the lower rubber plate 3 is cuboid, the upper rubber plate 2 is concave, the overall length and width of the upper rubber plate 2 and the lower stirring plate 3 are consistent, the upper rubber plate 2 and the lower stirring plate 3 are fastened with each other and then fixed by ultrasonic welding, and a cavity (not shown in the figure) with an opening at the top and capable of containing a gel is formed; four open grooves 4 are arranged at the lower end of the upper rubber plate 3 along the length direction, the open grooves 4 are communicated with the inside of the cavity and are used for connecting electrode grooves and being used as lower openings of electrophoresis conducting ports, the open grooves 4 are connected into a row, the intervals among the open grooves are equal, and removable sealing strips (not marked in the figure) are arranged on the open grooves 4; two cylindrical limiting columns 5 are respectively arranged on two side edges of the lower rubber plate 3 along the width direction of the lower rubber plate, and the height of each limiting column 5 represents the thickness of ultrasonic welding.
In order to ensure that the upper rubber plate 2 and the lower rubber plate 3 do not shake left and right or back and forth after being buckled, two U-shaped structure bulges 6 are respectively arranged on two side edges of the upper rubber plate 2 along the width direction, the open ends of the two U-shaped structure bulges 6 are oppositely arranged, correspondingly, a rectangular bulge A7 is respectively arranged on two side edges of the lower rubber plate 3 along the width direction, and the bulge A7 is buckled in a gap formed by the two U-shaped structure bulges 6.
The comb teeth 1 comprise a rack 8 and a comb body 9, the comb teeth 1 are covered on the top opening of the cavity, and the rack 8 is arranged towards the inside of the cavity; the length of the comb teeth 1 is less than that of the groove-shaped structure formed by the concave shape of the upper rubber plate 2, in order to accommodate the excess gel liquid extruded due to the expansion of the gel volume, a rectangular leakage groove 10 is arranged on the comb body 9 along the length direction, a channel 11 is arranged between the racks 8, the channel 11 is communicated with the leakage groove 10, and the excess gel liquid after extrusion can enter the leakage groove 10 along the channel 11 without causing the deformation of the formed gel.
In order to ensure that the positions of the comb teeth 1 inserted into the colloid are fixed, a cuboid-shaped bulge B12 is arranged on the surface, opposite to the liquid leakage groove 10, of the comb body 9 along the length direction of the comb body, the thickness of the bulge B12 is consistent with that of the upper rubber plate, after the comb teeth 1 are inserted into the cavity at a certain position, the bulge B12 is flush with the edge of the upper rubber plate, at the moment, the comb teeth 1 cannot continuously penetrate into the cavity, and the positions of the comb teeth 1 inserted into the gel at each time in later operation can be the same.
As shown in FIG. 6, as another preferred embodiment of the present invention, the present invention can also be provided with a plurality of grooves 13 along the length direction of the lower rubber plate 3 on the same side as the leakage groove 10, the vertical height of the grooves 13 on the lower rubber plate 3 is greater than the vertical height of the gel filled into the cavity and less than the vertical height of the top end of the comb teeth 1 inserted into the cavity, the grooves 13 can also contain a part of the gel liquid squeezed due to the expansion of the gel volume, and the gel in the gel preparation device can also be prevented from being deformed due to the expansion.
As another preferred embodiment of the present invention, a boundary line may be provided on the upper glue plate 2 or the lower glue plate 3, or on both glue plates from bottom to top at a height of 2/3-4/5 along the length direction thereof, the thickness of the glue plate below the boundary line is greater than that of the glue plate above the boundary line, the glue plates with different thicknesses at the boundary line are in arc-shaped transition arrangement, as shown in fig. 7, the opposite sides of the upper glue plate 2 and the lower glue plate 3 are both provided with boundary lines (not marked in the figure), and the thicknesses of the glue plates above and below the boundary lines are different, so that the upper half part of the cavity formed by the transition arrangement has a large volume and the lower half has a small volume; the problem that the rubber plate is deformed due to the overlarge volume of the upper half part of the liquid-transferring gun when the liquid-transferring gun stretches into the cavity can be effectively avoided.
When the gel preparation device is actually used, cleaning is not needed, acrylamide heavy liquid and light liquid which are prepared in advance are poured into a cavity in the gel preparation device, after the gel is added to a specified gel height, the comb teeth 1 are inserted into the gel, the liquid leakage groove 10 structure on the comb teeth 1 and the groove 13 structure on the gel plate can effectively prevent the gel from deforming, the sealing strips on the comb teeth 1 and the open groove 4 are pulled out before electrophoresis, and the gel plate is put into an electrophoresis device to carry out sample adding and electrophoresis.
It should be noted that the number of the racks 8 in the structural schematic diagram of the comb teeth 1 of the present invention is eight, but does not represent the number of the racks in actual production, and the number of the conventional racks 8 is 8 to 20.
Claims (10)
1. A gel preparation device for electrophoresis comprises a rubber plate and comb teeth, wherein the rubber plate comprises an upper rubber plate and a lower rubber plate which are mutually buckled to form a cavity with an opening at the top and capable of containing gel; the comb teeth comprise racks and comb bodies, the comb teeth are covered on the top opening of the cavity, and the racks face the inside of the cavity; the method is characterized in that: a rectangular liquid leakage groove is formed in the comb body along the length direction of the comb body, a channel is arranged between the racks, and the channel is communicated with the liquid leakage groove.
2. The apparatus for preparing gel for electrophoresis according to claim 1, wherein: the bottom and both sides fixed connection after upper rubber plate and lower rubber plate lock, the lower extreme of upper rubber plate or lower rubber plate is provided with two or more open slots, and the open slot communicates with each other with the cavity is inside for connect the electrode tank and as electrophoresis conducting port's under shed, be equipped with removable sealing strip on the open slot.
3. The apparatus for preparing gel for electrophoresis according to claim 2, wherein: the upper rubber plate and the lower rubber plate are fixed through ultrasonic welding.
4. The apparatus for preparing gel for electrophoresis according to claim 3, wherein: and a plurality of limiting columns are arranged on two sides of the upper rubber plate or the lower rubber plate and used for limiting the thickness of ultrasonic welding.
5. The apparatus for preparing gel for electrophoresis according to claim 1, wherein: two U-shaped structures are respectively arranged on two side edges of the upper rubber plate, the open ends of the two U-shaped structures are oppositely arranged, two rectangular bulges A are correspondingly arranged on the lower rubber plate, and the bulges A are buckled into a gap formed by the two U-shaped structures.
6. The apparatus for preparing gel for electrophoresis according to claim 1, wherein: the side of the comb body, which is opposite to the liquid leakage groove, is provided with a cuboid-shaped bulge B along the length direction, and the thickness of the bulge B is consistent with that of the adjacent rubber plate.
7. The apparatus for preparing gel for electrophoresis according to claim 1, wherein: the inner side of the rubber plate on the same side with the liquid leakage groove is provided with a plurality of grooves along the length direction, and the vertical height of the grooves on the rubber plate is greater than that of gel filled into the cavity and lower than that of the tail ends of the tops of the comb teeth inserted into the cavity; the shape of the groove can be round, diamond, square or rectangular.
8. The apparatus for preparing gel for electrophoresis according to claim 1, wherein: a boundary line is arranged on one side of the upper rubber plate, opposite to the lower rubber plate, from bottom to top at the height of 2/3-4/5 along the length direction of the upper rubber plate, the thickness of the upper rubber plate below the boundary line is larger than that of the upper rubber plate above the boundary line, and the upper rubber plates with different thicknesses on the boundary line are in arc-shaped transition arrangement.
9. The apparatus for preparing gel for electrophoresis according to claim 1, wherein: a boundary line is arranged on the side, opposite to the upper rubber plate, of the lower rubber plate from bottom to top at the height of 2/3-4/5 along the length direction of the lower rubber plate, the thickness of the lower rubber plate below the boundary line is larger than that of the lower rubber plate above the boundary line, and the lower rubber plates with different thicknesses on the boundary line are in arc-shaped transition arrangement.
10. The apparatus for preparing gel for electrophoresis according to claim 9, wherein: a boundary line is arranged on the upper rubber plate and at the same height position relative to the lower rubber plate along the direction of the boundary line, the thickness of the upper rubber plate below the boundary line is larger than that of the upper rubber plate above the boundary line, and the upper rubber plates with different thicknesses at the boundary line are in arc-shaped transition arrangement.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910592660.6A CN110215884A (en) | 2019-07-03 | 2019-07-03 | A kind of gel for electrophoresis preparation facilities |
| CN2019105926606 | 2019-07-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111558347A true CN111558347A (en) | 2020-08-21 |
Family
ID=67815714
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910592660.6A Pending CN110215884A (en) | 2019-07-03 | 2019-07-03 | A kind of gel for electrophoresis preparation facilities |
| CN202010178135.2A Pending CN111558347A (en) | 2019-07-03 | 2020-03-14 | Gel preparation facilities for electrophoresis |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910592660.6A Pending CN110215884A (en) | 2019-07-03 | 2019-07-03 | A kind of gel for electrophoresis preparation facilities |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN110215884A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06229983A (en) * | 1992-12-23 | 1994-08-19 | Bio Rad Lab Inc | Sample-well inserting member for forming wedge- shaped well in super-thin slab gell for electrophoresis |
| JPH08166370A (en) * | 1994-12-13 | 1996-06-25 | Norifumi Hirota | Electrophoresis device and supporter manufacturing apparatus for electrophoresis device |
| US6379519B1 (en) * | 1999-09-01 | 2002-04-30 | Mirador Dna Design Inc. | Disposable thermoformed electrophoresis cassette |
| CN203849203U (en) * | 2014-05-27 | 2014-09-24 | 李水清 | Sampler for agarose gel electrophoresis |
| US20150136605A1 (en) * | 2012-05-31 | 2015-05-21 | Ge Healthcare Bio-Sciences Ab | Electrophoresis tray and a method of running an electrophoresis experiment |
| CN104956214A (en) * | 2012-11-20 | 2015-09-30 | 南京金斯瑞生物科技有限公司 | Gel electrophoresis device for loading large sample volumes |
| US20160195493A1 (en) * | 2013-08-09 | 2016-07-07 | Philip Guadagno | Applicator comb for gel electrophoresis |
| CN107128864A (en) * | 2017-03-21 | 2017-09-05 | 上海从生生物科技有限公司 | The Automatic Control glue pouring system and method continuously made for gradient gel film |
| CN210332608U (en) * | 2019-07-03 | 2020-04-17 | 常州天地人和生物科技有限公司 | Gel preparation facilities for electrophoresis |
-
2019
- 2019-07-03 CN CN201910592660.6A patent/CN110215884A/en active Pending
-
2020
- 2020-03-14 CN CN202010178135.2A patent/CN111558347A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06229983A (en) * | 1992-12-23 | 1994-08-19 | Bio Rad Lab Inc | Sample-well inserting member for forming wedge- shaped well in super-thin slab gell for electrophoresis |
| JPH08166370A (en) * | 1994-12-13 | 1996-06-25 | Norifumi Hirota | Electrophoresis device and supporter manufacturing apparatus for electrophoresis device |
| US6379519B1 (en) * | 1999-09-01 | 2002-04-30 | Mirador Dna Design Inc. | Disposable thermoformed electrophoresis cassette |
| US20150136605A1 (en) * | 2012-05-31 | 2015-05-21 | Ge Healthcare Bio-Sciences Ab | Electrophoresis tray and a method of running an electrophoresis experiment |
| CN104956214A (en) * | 2012-11-20 | 2015-09-30 | 南京金斯瑞生物科技有限公司 | Gel electrophoresis device for loading large sample volumes |
| US20160195493A1 (en) * | 2013-08-09 | 2016-07-07 | Philip Guadagno | Applicator comb for gel electrophoresis |
| CN203849203U (en) * | 2014-05-27 | 2014-09-24 | 李水清 | Sampler for agarose gel electrophoresis |
| CN107128864A (en) * | 2017-03-21 | 2017-09-05 | 上海从生生物科技有限公司 | The Automatic Control glue pouring system and method continuously made for gradient gel film |
| CN210332608U (en) * | 2019-07-03 | 2020-04-17 | 常州天地人和生物科技有限公司 | Gel preparation facilities for electrophoresis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110215884A (en) | 2019-09-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2620504B2 (en) | Sample well insert for forming wedge-shaped wells in ultra-thin slab gel for electrophoresis | |
| US4035377A (en) | Slab gel electrophoresis apparatus | |
| DE68919375T2 (en) | Electrophoresis device. | |
| DE69828153T2 (en) | INTEGRATED CLOSED SECONDARY BATTERY | |
| US6905584B2 (en) | Electrophoresis system and method therefor | |
| CN209895111U (en) | Adjustable liquid crystal display rubber frame | |
| US4385974A (en) | Electrophoretic system and method for multidimensional analysis | |
| EP3168610A1 (en) | Electrophoresis gel cassette and comb | |
| CN107546541A (en) | Electric connector and its manufacture method | |
| CN210332608U (en) | Gel preparation facilities for electrophoresis | |
| US5989403A (en) | Electrophoresis assembly with filling means | |
| CN111558347A (en) | Gel preparation facilities for electrophoresis | |
| US20190151779A1 (en) | Modular backwash assembly and method for using the same | |
| US5304292A (en) | Electrophoresis gels | |
| JP6196680B2 (en) | Gel electrophoresis device for loading large samples | |
| US5232573A (en) | Integral electrophoresis gel form | |
| CN1034262A (en) | Plate heat exchanger | |
| CN116223797B (en) | Colloidal gold detection device and detection method | |
| CN218445260U (en) | Gel preparation facilities for electrophoresis | |
| CN210906099U (en) | Gel preparation facilities of SDS-PAGE | |
| DE19930253A1 (en) | Chamber for two-dimensional electrophoresis using stacked horizontal includes at least two guide members for inserting an isolating element for temporarily separating two gels | |
| JP5108146B2 (en) | Welded electrophoresis cassette without leaking weld line | |
| US20220281913A1 (en) | Intragel well sample loading system | |
| CN110586002A (en) | Gel preparation facilities of SDS-PAGE | |
| CN219377084U (en) | Structure for enhancing support of SDS-PAGE gel column |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |