CN219377084U

CN219377084U - Structure for enhancing support of SDS-PAGE gel column

Info

Publication number: CN219377084U
Application number: CN202320557054.2U
Authority: CN
Inventors: 王杰; 郭惠芳; 祁艳芳; 冷兆武
Original assignee: Nanjing Youai Biotechnology R & D Co ltd
Current assignee: Nanjing Youai Biotechnology R & D Co ltd
Priority date: 2023-03-21
Filing date: 2023-03-21
Publication date: 2023-07-21
Anticipated expiration: 2033-03-21

Abstract

The utility model discloses a structure for enhancing the supportability of an SDS-PAGE gel column, and belongs to the technical field of electrophoresis devices. The structure for enhancing the supportability of the SDS-PAGE gel column comprises a gel preparation front plate, a gel preparation rear plate and a gel preparation comb which are matched, wherein the gel preparation comb comprises comb teeth, comb teeth gaps are formed between the comb teeth, and grooves are preset at the overlapping positions of the gel preparation front plate and/or the gel preparation rear plate and the comb teeth gaps before the gel preparation is matched with gel; in a specific gel process, liquid glue solution flows into the groove, the glue solution is filled in the groove, and after the glue solution is condensed into solid, the solid glue solution and the glue column form an integrated structure synchronously, so that the supportability of the glue column is greatly enhanced. Solves the problem that the gel column is bent due to insufficient strength in the existing SDS-PAGE gel.

Description

Structure for enhancing support of SDS-PAGE gel column

Technical Field

The utility model belongs to the technical field of electrophoresis devices, and particularly relates to a structure for enhancing the supportability of an SDS-PAGE gel column.

Background

Gel electrophoresis is a technique commonly used for separation of biomolecules such as proteins and nucleic acids (DNA and RNA). Typically, the method requires the application of an electrical current to the porous polymeric gel medium containing the biological sample. The sample components typically migrate at different rates in the gel medium due to their different charges and/or sizes. Migration of the molecules through the polymeric gel medium forms a series of bands, with different bands corresponding to different molecules.

Polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for separating biomolecules such as proteins and nucleic acids. The polyacrylamide gel is a gel with a three-dimensional network structure formed by polymerizing and crosslinking monomer acrylamide and crosslinking agent N, N-methylene bisacrylamide under the action of accelerator N, N, N, N-tetramethyl ethylenediamine and catalyst ammonium persulfate or riboflavin, wherein the pore size of the three-dimensional network structure of the gel is similar to that of biological macromolecules, so that the gel has a molecular sieve effect; the separation using these gels depends not only on the charge density of the macromolecules, but also on the size of the molecules.

At present, most of glass-made rubber plates for laboratories are used as carriers of polyacrylamide gel; for the convenience of transportation and cost saving, most of commercial polyacrylamide gel is made of plastic instead of glass.

In the prior art, when SDS-PAGE gel is used, as shown in fig. 1, firstly, stripping comb teeth, leaking a sample loading hole (6), and when the comb teeth are pulled out, some glue columns (7) are bent and lodged due to insufficient strength, so that the subsequent sample loading is affected.

Disclosure of Invention

1. Problems to be solved

Aiming at the problem that the gel column is bent due to insufficient strength in the existing SDS-PAGE gel, the utility model provides a structure for enhancing the supportability of the SDS-PAGE gel column. By adopting the technical scheme of the utility model, the support of the rubber column can be effectively enhanced without affecting the function of the polyacrylamide gel.

2. Technical proposal

In order to solve the problems, the technical scheme adopted by the utility model is as follows:

the utility model relates to a structure for enhancing the supportability of an SDS-PAGE gel column, which comprises a gel preparation front plate, a gel preparation rear plate and a gel preparation comb which are matched, wherein the gel preparation comb comprises comb teeth, comb tooth gaps are arranged between the comb teeth, and grooves are preset at the overlapping positions of the gel preparation front plate and/or the gel preparation rear plate and the comb tooth gaps before the gel preparation is matched with gel; in a specific gel process, liquid glue solution flows into the groove, the glue solution is filled in the groove, and after the glue solution is condensed into solid, the solid glue solution and the glue column form an integrated structure synchronously, so that the supportability of the glue column is greatly enhanced.

Further, the area of the groove is smaller than or equal to the area of the gap between the comb teeth, the area of the groove is large, the volume of the finally formed reinforced rubber column is large, and the rubber column is not easy to break when the comb teeth are pulled out.

Furthermore, the number of the corresponding grooves at each comb tooth gap is two or more, so that a multi-point support can be formed, and the strength of the rubber column is further enhanced.

Further, the depth of the groove is at least 0.03mm, and theoretically, in the thickness range of the front plate of the gel forming tool and the rear plate of the gel forming tool, the deeper the groove is, the greater the strength of the rubber column is, and the depth is at least 0.03mm for ensuring the strength of the rubber column.

Furthermore, the grooves are round, cone-shaped, square or polygonal, the corresponding shapes can be selected according to the different thickness requirements of the gel, and arc transition is preferably arranged at the corners of the grooves, so that the glue solution can be fully filled, and the strength of the glue column is improved.

3. Advantageous effects

Compared with the prior art, the utility model has the beneficial effects that:

compared with SDS-PAGE gel without grooves, the structure for enhancing the support property of the SDS-PAGE gel column has the advantages that the support force of the gel column is greatly enhanced, and after comb teeth are pulled out, the gel column is not easy to bend, so that the subsequent loading is not influenced.

Drawings

FIG. 1 is a schematic diagram of a sample loading hole and a glue column formed after the comb teeth of a conventional gel manufacturing tool are pulled out;

FIG. 2 is a schematic structural view of an enhanced SDS-PAGE gel column according to the present utility model;

FIG. 3 is a schematic view of a back plate structure of the gel mold according to the present utility model;

FIG. 4 is an enlarged partial rear side view of a prior art formed glue string;

FIG. 5 is an enlarged partial rear side view of a glue string formed in accordance with the present utility model;

FIG. 6 is an enlarged partial rear side view of a glue string formed in accordance with the present utility model;

FIG. 7 is an enlarged partial rear side view of a glue string formed in accordance with the present utility model;

fig. 8 is an enlarged partial rear side view of a glue string formed in accordance with the present utility model.

In the figure: 1. a gel mold front plate; 2. a gel mold back plate; 3. a groove; 4. comb teeth gaps; 5. gel preparation comb; 6. a sample loading hole; 7. a glue column; 51. comb teeth.

Detailed Description

The utility model is further described below in connection with specific embodiments.

Example 1

The structure for enhancing the supportability of the SDS-PAGE gel column of the present embodiment includes, as shown in fig. 2 and 3, a front plate 1 of the gel forming tool, a rear plate 2 of the gel forming tool, and a comb 5 of the gel forming tool, which are generally configured in a complete set, where the comb 5 of the gel forming tool includes comb teeth 51, comb teeth gaps 4 are formed between the comb teeth 51, and a groove 3 is preset at a position where the front plate 1 of the gel forming tool and/or the rear plate 2 of the gel forming tool overlap with the comb teeth gaps 4 before the gel forming tool cooperates with the gel, that is, the groove 3 may be reserved at a position where the front plate 1 of the gel forming tool overlaps with the comb teeth gaps 4, or may be reserved at a position where the rear plate 2 of the gel forming tool overlaps with the comb teeth gaps 4.

In the structure for enhancing the supportability of the SDS-PAGE gel column, in a specific gel process, liquid glue flows into the groove 3, the groove 3 is filled with the glue, and after the glue is coagulated into solid, the solid and the gel column 7 are synchronously integrated; the grooves 3 are reserved on the front plate 1 of the gel manufacturing tool and the rear plate 2 of the gel manufacturing tool, and the formed rubber columns 7 are respectively shown in fig. 5 and 6, so that the support of the rubber columns 7 is greatly enhanced compared with the rubber column 7 structure shown in fig. 4 in the prior art; of course, when grooves 3 are reserved on the front plate 1 of the gel mold and the rear plate 2 of the gel mold, as shown in fig. 7, the formed rubber columns 7 are supported on two sides, and the strength is higher.

Example 2

The basic structure of the structure for enhancing the supportability of an SDS-PAGE gel column in the embodiment is the same as that in the embodiment 1, and the difference or improvement is that: the area of the recess 3 is smaller than or equal to the area of the comb teeth gap 4, preferably equal to the area of the comb teeth gap 4, with the advantage that: the area of the groove 3 is large, the volume of the finally formed reinforced rubber column 7 is large, and the rubber column 7 is not easy to break when the comb teeth 51 are pulled out. The number of the corresponding grooves 3 at each comb gap 4 is two or more, as shown in fig. 8, and the formed glue posts 7 can form multi-point supports, so that the strength of the glue posts 7 is further enhanced. The depth of the groove 3 is at least 0.03mm, and in theory, in the thickness range of the front plate 1 of the gel manufacturing tool and the rear plate 2 of the gel manufacturing tool, the deeper the groove 3 is, the stronger the strength of the rubber column 7 is, and the depth is at least 0.03mm in order to ensure the strength of the rubber column 7. The shape of the groove 3 is round, cone-shaped, square or polygonal, the corresponding shape can be selected according to the different thickness requirements of the gel, and the corners of the groove 3 are preferably provided with arc transition, so that the glue solution can be fully filled to improve the strength of the glue column 7.

Compared with an SDS-PAGE gel without adding grooves, the structure for enhancing the supportability of the SDS-PAGE gel column has the advantages that the supporting force of the gel column is greatly enhanced, after comb teeth are pulled out, the gel column is not easy to bend, the subsequent loading is not affected, and the function of polyacrylamide gel is not affected.

The utility model and its embodiments have been described above by way of illustration and not limitation, and the utility model is illustrated in the accompanying drawings and described in the drawings in which the actual structure is not limited thereto. Therefore, if one of ordinary skill in the art is informed by this disclosure, the structural mode and the embodiments similar to the technical scheme are not creatively designed without departing from the gist of the present utility model.

Claims

1. The utility model provides a structure of reinforcing SDS-PAGE glue post supportability, includes gel system utensil front bezel (1), gel system utensil back plate (2) and gel system utensil comb (5) that the cooperation set up, gel system utensil comb (5) include broach (51), are broach clearance (4) between each broach (51), its characterized in that: before each gel mold is matched with gel, grooves (3) are preset at the positions of the front plate (1) of the gel mold and/or the rear plate (2) of the gel mold, which are overlapped with the comb gaps (4).

2. The structure for enhancing the support of an SDS-PAGE gel column of claim 1, wherein: the area of the groove (3) is smaller than or equal to the area of the comb gap (4).

3. The structure for enhancing the support of an SDS-PAGE gel column of claim 1, wherein: the number of the corresponding grooves (3) at each comb gap (4) is two or more.

4. A structure for enhancing the support of an SDS-PAGE gel column according to any of claims 1 to 3, characterized in that: the depth of the groove (3) is at least 0.03mm.

5. The SDS-PAGE gel column supporting structure of claim 4, wherein: the shape of the groove (3) is round, cone-shaped, square or polygonal.