CN111494300A - Cosmetic composition with comprehensive oxidation resistance and preparation method thereof - Google Patents

Abstract

The invention discloses a cosmetic composition with comprehensive oxidation resistance and a preparation method thereof. The cosmetic composition comprises the following components in percentage by mass: 0.01-10% of schizosaccharomyces cerevisiae fermentation product lysate and 0.01-2% of lactobacillus fermentation product; 0.01-2% of procyanidolic inclusion before cysteinyl oligomerization; extract of leaves/stems of Artocarpus heterophyllus 0.01-10%; 86-99.6% of cosmetic auxiliary material matrix. The invention is characterized in that aiming at an oxidation formation mechanism, the self resistance and repair capability of the skin are improved, the microbial flora on the surface of the skin is maintained, the endogenous antioxidant enzyme activity system of the skin is activated, and the inclusion is used for directionally removing free radicals to achieve the safe, effective and comprehensive antioxidant effect.

Description

Cosmetic composition with comprehensive oxidation resistance and preparation method thereof

Technical Field

The invention relates to a skin care product, belongs to the technical field of cosmetics, and particularly relates to a cosmetic composition with comprehensive oxidation resistance and a preparation method thereof.

Background

The oxidative damage of cells and tissues, which is manifested by adverse phenomena such as fine lines, wrinkles, pigmentation, yellow smell, large pores, dryness, roughness, allergy and the like, is caused by a large amount of redundant free radicals generated by external electromagnetic radiation, air pollution, ultraviolet irradiation, microorganisms, and in-vivo autoxidation and enzymatic oxidation.

The prior antioxidants mainly comprise vitamins such as vitamin C, E, β -carotene, coenzyme Q10, superoxide dismutase, anthocyanin and the like, however, the active ingredients of the antioxidants are difficult to store in the process of manufacturing, storing and transporting, the antioxidant performance can not meet the requirement, and the stability problems such as color change and the like exist.

Some problems commonly exist in the antioxidant skin care products on the market, such as: the antioxidant mechanism is single and not comprehensive enough, the antioxidant effect is not good, the product stability is not good, and the irritation is large.

Therefore, there is a need for a cosmetic composition that is fully antioxidant.

Disclosure of Invention

Aiming at an oxidation formation mechanism, the invention can improve the self resistance and repair capability of the skin, maintain the microbial flora on the surface of the skin, activate the endogenous antioxidant enzyme activity system of the skin, and directionally remove free radicals by using an inclusion body to achieve the safe, effective and comprehensive antioxidant effect.

The cosmetic composition with comprehensive oxidation resistance is realized by the following technical scheme.

The cosmetic composition for comprehensive oxidation resistance comprises the following components in percentage by mass:

0.01-10% of a yeast secondary fission fermentation product lysate; 0.01-2% of lactobacillus fermentation product; 0.01-2% of cysteinyl oligomeric proanthocyanidin capsule inclusion; extract of leaves/stems of Artocarpus heterophyllus 0.01-10%; 86-99.6% of cosmetic auxiliary material matrix.

The comprehensive antioxidant cosmetic composition is preferably determined by combining cost factors, the specificity of the functions of the components and the safe concentration of the components, and comprises the following components in percentage by mass:

0.5-10% of a yeast secondary fission fermentation product lysate; 0.05-2% of lactobacillus fermentation product; 0.05-1% of cystoyl oligomeric proanthocyanidin capsule inclusion; extract of leaves/stems of Artocarpus heterophyllus 0.5-5%; 82-98.9% of cosmetic auxiliary material matrix.

Further, the cysteinyl oligomeric proanthocyanidin capsule inclusion comprises the following components in percentage by weight:

80-90% of water, 3-6% of butanediol, 1-3% of hydrolyzed procyanidin, 1-2% of polyvinylpyrrolidone, 0.5-1.5% of hydroxyethyl cellulose, 0.5-1% of phenoxyethanol, 0.3-0.9% of sodium alginate, 0.05-0.15% of chitosan, 0.05-0.1% of cysteine, 0.03-1% of cetearyl glucoside, 0.01-0.05% of poly gamma-sodium glutamate and 0.005-0.02% of sodium hyaluronate.

Further, the cosmetic auxiliary material matrix of the present invention comprises: one or more of a solvent, a humectant, a preservative, an emollient, an emulsifier, a thickener, a chelating agent, a fragrance, a pH adjuster, a skin conditioner.

Further, the cosmetic auxiliary material matrix comprises the following components in percentage by mass:

63.6 to 99.77 percent of solvent; 0-10% of humectant; 0.2 to 1 percent of preservative; 0-10% of an emollient; 0-3% of emulsifier; 0-1% of thickening agent; 0.03-0.2% of chelating agent; 0-0.2% of aromatic; 0-1% of pH regulator; 0-10% of skin conditioner.

The solvent is selected from one or more of deionized water, plant extract, flower water and fermentation liquor.

The humectant is selected from one or more of glycerol, propylene glycol, butanediol, dipropylene glycol, isoprene glycol, 1, 3-propylene glycol, methyl propylene glycol, polyethylene glycol-32, methyl glucitol polyether-20, glycerol acrylate/acrylic acid copolymer, and glycerol polyether-26.

The preservative is selected from one or more of parabens, phenoxyethanol, chlorphenesin, caprylyl hydroximic acid, glyceryl caprylate, benzoic acid and salts thereof, dehydroacetic acid and salts thereof, 1, 2-pentanediol, ethylhexyl glycerol and caprylyl glycol.

The emollient is selected from one or more of squalane, dimethicone, mineral oil, cetostearyl alcohol, myristyl myristate, shea butter, olive oil, isopropyl palmitate, ethylhexyl palmitate, dioctyl carbonate, tri (ethyl hexanoate) glyceride, caprylic/capric triglyceride.

The emulsifier is selected from one or more of span, polyethylene glycol, tween, glycoside, lecithin, glyceride, sucrose ester, stearyl alcohol polyether, polyglycerol ester, and amino acid.

The thickening agent is selected from one or more of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, carbomer, xanthan gum, carrageenan, cellulose, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, polyacrylic acid and salts thereof, sodium polyacrylate grafted starch, sclerotium rolfsii (SC L EROTIUM RO L FSSII) glue and gellan gum.

The chelating agent is selected from one or more of EDTA-disodium, EDTA-tetrasodium and combinations thereof.

The pH regulator is one or more selected from citric acid, sodium citrate, sodium hydroxide, potassium hydroxide, triethanolamine, aminomethyl propanol, arginine, acetic acid and lactic acid.

The skin conditioner is selected from one or more of plant extract, fermentation product, fermentation filtrate, peptide, hyaluronic acid, and polyglutamic acid.

In addition, the invention also provides a preparation method of the comprehensive antioxidant cosmetic composition, which comprises the following process steps:

1) adding the water-soluble high-temperature-resistant material body into a water phase pot, stirring and heating to 80-85 ℃, and keeping the temperature and stirring for 20-25 minutes;

2) adding the oil-soluble high-temperature-resistant material body into an oil pan, stirring and heating to 80-85 ℃, and keeping the temperature and stirring for 10-15 minutes;

3) pumping the water phase pot material body into an emulsifying pot through a 200-mesh filter screen, stirring and homogenizing, pumping the oil phase pot material body into the emulsifying pot, stirring and homogenizing for 5-10 minutes, and keeping the temperature and stirring for 10-15 minutes;

4) cooling to 60-65 deg.C, adding pH regulator, stirring for 10-15 min;

5) cooling to 40-45 deg.C, and stirring the rest skin conditioner, antiseptic and aromatic for 10-15 min;

6) stirring and cooling to a temperature lower than 38 ℃, stopping stirring, and discharging after the detection is qualified.

Furthermore, the cosmetic composition with comprehensive oxidation resistance can be used for various cosmetic preparations, including but not limited to astringent, skin care essence, skin care emulsion, skin care cream, color cosmetics and cleansing cream.

The main raw material effects of the invention are illustrated as follows:

① Zymobacter bifidus fermentation product lysate

Derived from a bifidobacterium lysate.

The yeast lysate of the binary split yeast fermentation product comprises metabolic products, cytoplasmic components, cell wall components, polysaccharide complexes. Can regulate immune system, prevent damage caused by ultraviolet rays, and provide preventive care for skin.

② lactic acid bacteria fermentation product

The lactobacillus fermentation product can promote the production of staphylococcus epidermidis (Mycosphaeus) and maintain the normal balance of skin bacteria. Phagocytizing and decomposing sebum to generate acidic substances, keeping skin weakly acidic, and inhibiting reproduction of infectious microbes; promoting the production of glycerin and moistening skin.

③ Capsule inclusion of procyanidolic oligomers of cysteinyl

The cysteinyl pre-oligomeric proanthocyanidin in the cysteinyl pre-oligomeric proanthocyanidin capsule inclusion is extracted from litchi.

The cysteinyl oligomeric proanthocyanidin capsule inclusion comprises the following components in percentage by weight:

80-90% of water, 3-6% of butanediol, 1-3% of hydrolyzed procyanidin, 1-2% of polyvinylpyrrolidone, 0.5-1.5% of hydroxyethyl cellulose, 0.5-1% of phenoxyethanol, 0.3-0.9% of sodium alginate, 0.05-0.15% of chitosan, 0.05-0.1% of cysteine, 0.03-1% of cetearyl glucoside, 0.01-0.05% of poly gamma-sodium glutamate and 0.005-0.02% of sodium hyaluronate.

The cysteinyl pre-oligomeric proanthocyanidin capsule inclusion has a detection function, can release the cysteinyl pre-oligomeric proanthocyanidin to problematic skin, and exerts the maximum antioxidant effect.

④ Artocarpus heterophyllus leaf/stem extract

The extract of leaves/stems of Artocarpus heterophyllus is derived from dead grass and living algae during flowering.

The extract of leaves/stems of Artocarpus heterophyllus is rich in natural glycerol glucoside, and can enhance cell activity and metabolism, and activate endogenous antioxidase activity of cells.

Compared with the prior art, the invention has the following characteristics and effects:

1) the lysate of the yeast fermentation product of the second split can regulate and control an immune system, prevent the damage caused by ultraviolet rays and provide preventive care (aiming at external ultraviolet rays) for skin; the lactobacillus fermentation product can maintain the balance of the skin normal bacteria (aiming at the change of external microbial flora); the cysteinyl pre-oligomeric proanthocyanidin capsule inclusion has a detection function, and can release active ingredients at fixed points to eliminate redundant free radicals (aiming at internal oxidation); the extract of leaves/stems of Artocarpus heterophyllus activates the endogenous antioxidant enzyme activity of the cells (against intrinsic autooxidation). The four components supplement each other and inhibit each stage of oxidation formation (external and internal autooxidation), so as to achieve comprehensive antioxidant effect.

2) The cosmetic composition with comprehensive oxidation resistance can improve the self resistance and repair capability of skin, maintain the microbial flora on the surface of the skin, activate the endogenous antioxidant enzyme activity system of the skin and directionally remove free radicals by using the inclusion to achieve safe, effective and comprehensive oxidation resistance aiming at an oxidation formation mechanism.

Detailed Description

The present invention is described below by referring to some specific preferred embodiments in combination with effect test examples, which are only used to further illustrate the present invention and are not intended to limit the present invention, and do not represent a limitation to the scope of the present invention. Other insubstantial modifications and adaptations of the present invention can be made without departing from the scope of the present invention.

Unless otherwise specified, the following percentages (%) are mass percentages.

Examples 1 to 7

Table 1 shows the components of specific examples 1-7 of the cosmetic composition of the present invention for overall antioxidant effect.

TABLE 1 examples 1-7 Components (wt%)

The pH adjusting agent in Table 1 above is selected from triethanolamine, acetic acid, lactic acid. The antiseptic is selected from phenoxyethanol, 1, 2-pentanediol, and sodium benzoate.

The preparation of examples 1-7 above was as follows:

1) adding water, EDTA disodium and carbomer into a water phase pot, stirring and heating to 80-85 ℃, and keeping the temperature and stirring for 20-25 minutes;

2) cooling to 60-65 deg.C, adding triethanolamine, stirring for 10-15 min;

3) cooling to 40-45 deg.C, adding the rest phenoxyethanol, split yeast fermentation product lysate, lactobacillus fermentation product, cysteinyl oligomeric proanthocyanidin capsule inclusion, Artocarpus heterophyllus leaf/stem extract, 1, 2-pentanediol, acetic acid, lactic acid, phenoxyethanol, and sodium benzoate, and stirring for 10-15 min;

4) stirring and cooling to a temperature lower than 38 ℃, stopping stirring, and discharging after the detection is qualified.

Test example for Effect verification

Cytotoxicity Effect test example 1

1) The test method comprises the following steps:

collecting human fibroblast cells (HFF-1) with logarithmic phase growth, inoculating on E-plate 16 instrument, culturing on multifunctional cell real-time cell analyzer (RTCA) instrument for 24 hr, adding the above-mentioned materials of examples 1-7, using untreated group as control group, placing in RTCA instrument, 37 deg.C, and 5% CO₂After 48 hours of culture, cell survival was observed.

2) And (3) testing results:

the cell activities of examples 1-7 of the invention are all greater than 80%, i.e., no toxicity to cells can be considered, and the cell can be directly used for ROS test of antioxidant capacity.

Oxidation resistance Effect test 2

1) The test method comprises the following steps:

human fibroblasts (HFF-1) grown in logarithmic phase were collected and inoculated into 96-well plates and cultured for 24 hours, and each of examples 1 to 7 was added, and the ROS content was measured using an ROS kit after 24 hours of culture, using an untreated group as a negative control and 100. mu.g/ml VC as a positive control.

2) And (3) testing results:

the ROS content sequence is as follows: negative control > example 1> positive control > example 7> example 6> example 2> example 3> example 5> example 4.

It can be seen that the antioxidant capacity is in the order: negative control group < example 1< positive control < example 7< example 6< example 2< example 3< example 5< example 4.

Example 5 has four major components all in higher amounts than example 4 but the ability to scavenge free radicals is less good than example 4. It is not stated that the higher the amount added, the better.

Safety test 3

1) The test method comprises the following steps:

the skin test of examples 1 to 7 was performed, a qualified spot tester was selected, and according to a closed spot test method, about 0.020 to 0.025m L of the sample was placed in the spot tester, and a medical tape was applied to the back of the subject, and after 24 hours, the subject was removed, and skin reactions were observed for 0.5 hour, 24 hours, and 48 hours after the spot removal, and the results were recorded according to the skin reaction grading standard in the technical safety standards for cosmetics (2015 edition).

2) The number of tested persons:

each group had 30 healthy people, 15 men and 15 women; the age range is 20-45 years.

3) Evaluation criteria:

the skin adverse effect grading criteria are shown in table 2 below.

TABLE 2 grading Standard of adverse skin reactions

4) And (3) testing results:

the human skin closed patch test shows that 0 of 30 persons in each group have positive reactions, and the examples 1-7 do not cause adverse skin reactions to the batch of subjects according to the regulation in the technical Specification for cosmetic safety (2015).

Synergistic test example (example 2 and comparative examples 1 to 6)

Table 3 shows the specific example 2 and comparative examples 1-6 components of the overall antioxidant composition.

Table 3 example 2 and comparative examples 1-6 components (wt%)

In table 3 above, the pH adjusting agent is selected from triethanolamine, acetic acid, lactic acid. The antiseptic is selected from phenoxyethanol, 1, 2-pentanediol, and sodium benzoate.

The preparation methods of the above example 2 and comparative examples 1 to 6 were as follows:

1) adding water, EDTA disodium and carbomer into a water phase pot, stirring and heating to 80-85 ℃, and keeping the temperature and stirring for 20-25 minutes;

2) cooling to 60-65 deg.C, adding triethanolamine, stirring for 10-15 min;

3) cooling to 40-45 deg.C, adding the rest phenoxyethanol, split yeast fermentation product lysate, lactobacillus fermentation product, cysteinyl oligomeric proanthocyanidin capsule inclusion, Artocarpus heterophyllus leaf/stem extract, 1, 2-pentanediol, acetic acid, lactic acid, phenoxyethanol, and sodium benzoate, and stirring for 10-15 min;

4) stirring and cooling to a temperature lower than 38 ℃, stopping stirring, and discharging after the detection is qualified.

The example 2 and the comparative examples 1 to 6 were subjected to the antioxidant effect test, and the results are shown in the following table 4.

TABLE 4 example 2 and comparative examples 1-6 results in oxidation resistance

Sample (I)

Example 2

Comparative example 1

Comparative example 2

Comparative example 3

Comparative example 4

Comparative example 5

Comparative example 6

ROS content

72.01％

93.11％

90.22％

90.11％

88.82％

88.24％

88.13％

As can be seen from table 4, the order of antioxidant capacity is: example 2> comparative example 6> comparative example 5> comparative example 4> comparative example 3> comparative example 2> comparative example 1.

Oxidation resistance: example 2> comparative example 1+ comparative example 4. Comparative example 1 contains only 2 major antioxidant ingredients, mainly to protect against external damage. Comparative example 4 contained only 2 major antioxidant components, primarily for endogenous oxidation. When the antioxidant is singly used for external damage or endogenous oxidation, the antioxidant effect is not outstanding. The antioxidant effect of both (i.e., example 2) is much better than the combined effect of both.

Oxidation resistance: example 2> comparative example 3+ comparative example 6. Comparative example 3 contains only 2 antioxidant components mainly against external damage, and the content of the corresponding antioxidant components is higher than that in example 2; comparative example 6 contained only 2 major antioxidant components, primarily for endogenous oxidation, and the corresponding antioxidant content was high for example 2. Theoretically, the sum of the antioxidant effects of comparative examples 3 and 6 is higher than that of comparative example 2, and it can be actually said that the antioxidant effect of comparative example 2 is higher than that of comparative examples 3 and 6. This is an unforeseeable effect.

The effect of single target on resisting external damage or single target on endogenous oxidation is not superior to the compound synergistic effect of the internal and external aspects.

In conclusion, the comprehensive antioxidant cosmetic composition achieves comprehensive and efficient antioxidant effects through synergistic interaction among the components.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims (8)

1. The cosmetic composition with comprehensive oxidation resistance is characterized by comprising the following components in percentage by mass:

0.01-10% of a yeast secondary fission fermentation product lysate; 0.01-2% of lactobacillus fermentation product; 0.01-2% of cysteinyl oligomeric proanthocyanidin capsule inclusion; extract of leaves/stems of Artocarpus heterophyllus 0.01-10%; 86-99.6% of cosmetic auxiliary material matrix.

2. The cosmetic composition with complete antioxidation according to claim 1, characterized by comprising the following components by mass percent:

0.5-10% of schizosaccharomyces cerevisiae fermentation product lysate and 0.05-2% of lactobacillus fermentation product; 0.05-1% of cysteinyl oligomerization proanthocyanidin capsule inclusion; extract of leaves/stems of Artocarpus heterophyllus 0.5-5%; 82-98.9% of cosmetic auxiliary material matrix.

3. The cosmetic composition with total antioxidant effect as set forth in claim 1 or 2, wherein: the cysteinyl oligomeric proanthocyanidin capsule inclusion is composed of the following components in percentage by weight:

80-90% of water, 3-6% of butanediol, 1-3% of hydrolyzed procyanidin, 1-2% of polyvinylpyrrolidone, 0.5-1.5% of hydroxyethyl cellulose, 0.5-1% of phenoxyethanol, 0.3-0.9% of sodium alginate, 0.05-0.15% of chitosan, 0.05-0.1% of cysteine, 0.03-1% of cetearyl glucoside, 0.01-0.05% of poly gamma-sodium glutamate and 0.005-0.02% of sodium hyaluronate.

4. The cosmetic composition with total antioxidant effect of claim 3, wherein: the cosmetic auxiliary material matrix is selected from one or more of solvent, humectant, antiseptic, emollient, emulsifier, thickener, chelating agent, aromatic, pH regulator, and skin conditioner.

5. The cosmetic composition with total antioxidant effect of claim 4, wherein: the cosmetic auxiliary material matrix comprises the following components in percentage by mass:

63.6 to 99.77 percent of solvent; 0-10% of humectant; 0.2 to 1 percent of preservative; 0-10% of an emollient; 0-3% of emulsifier; 0-1% of thickening agent; 0.03-0.2% of chelating agent; 0-0.2% of aromatic; 0-1% of pH regulator; 0-10% of skin conditioner.

6. The cosmetic composition with total antioxidant effect of claim 5, wherein:

the solvent is selected from one or more of deionized water, plant extract, flower water and fermentation liquor.

The humectant is selected from one or more of glycerol, propylene glycol, butanediol, dipropylene glycol, isoprene glycol, 1, 3-propylene glycol, methyl propylene glycol, polyethylene glycol-32, methyl glucitol polyether-20, glycerol acrylate/acrylic acid copolymer and glycerol polyether-26;

the preservative is selected from one or more of parabens, phenoxyethanol, chlorphenesin, caprylyl hydroximic acid, glyceryl caprylate, benzoic acid and salts thereof, dehydroacetic acid and salts thereof, 1, 2-pentanediol, ethylhexyl glycerol and caprylyl glycol;

the emollient is selected from one or more of squalane, dimethicone, mineral oil, cetostearyl alcohol, myristyl myristate, shea butter, olive oil, isopropyl palmitate, ethylhexyl palmitate, dioctyl carbonate, tris (ethylhexanoate) glyceride, caprylic/capric triglyceride;

the emulsifier is selected from one or more of span, polyethylene glycol, tween, glycoside, lecithin, glyceride, sucrose ester, stearyl alcohol polyether, polyglycerol ester and amino acid;

the thickening agent is selected from one or more of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, carbomer, xanthan gum, carrageenan, cellulose, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, polyacrylic acid and salts thereof, sodium polyacrylate grafted starch, sclerotium rolfsii (SC L EROTIUM RO L FSSII) glue and gellan gum;

the chelating agent is selected from one or more of disodium EDTA, tetrasodium EDTA and the combination thereof;

the pH regulator is selected from one or more of citric acid, sodium citrate, sodium hydroxide, potassium hydroxide, triethanolamine, aminomethyl propanol, arginine, acetic acid and lactic acid;

the skin conditioner is selected from one or more of plant extract, fermentation product, fermentation filtrate, peptide, hyaluronic acid, and polyglutamic acid.

7. Process for the preparation of a cosmetic composition with total antioxidant capacity according to any of claims 4 to 6, characterized in that it comprises the following process steps:

1) adding the water-soluble high-temperature-resistant material body into a water phase pot, stirring and heating to 80-85 ℃, and keeping the temperature and stirring for 20-25 minutes;

2) adding the oil-soluble high-temperature-resistant material body into an oil pan, stirring and heating to 80-85 ℃, and keeping the temperature and stirring for 10-15 minutes;

3) pumping the water phase pot material body into an emulsifying pot through a 200-mesh filter screen, stirring and homogenizing, pumping the oil phase pot material body into the emulsifying pot, stirring and homogenizing for 5-10 minutes, and keeping the temperature and stirring for 10-15 minutes;

4) cooling to 60-65 deg.C, adding pH regulator, stirring for 10-15 min;

5) cooling to 40-45 deg.C, and stirring the rest skin conditioner, antiseptic and aromatic for 10-15 min;

6) stirring and cooling to a temperature lower than 38 ℃, stopping stirring, and discharging after the detection is qualified.

8. The method of preparing a full scale antioxidant cosmetic composition of claim 7, wherein: the cosmetic composition can be used for various cosmetic skin care preparations, including but not limited to astringent, skin care essence, skin care emulsion, skin care cream, color cosmetics and cleansing cream.