CN111487231B - Plant germ rapid detection device - Google Patents
Plant germ rapid detection device Download PDFInfo
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- CN111487231B CN111487231B CN202010311378.9A CN202010311378A CN111487231B CN 111487231 B CN111487231 B CN 111487231B CN 202010311378 A CN202010311378 A CN 202010311378A CN 111487231 B CN111487231 B CN 111487231B
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- light guide
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- ultraviolet
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application discloses a plant germ rapid detection device, which comprises: the kit comprises a kit body, a light guide body arranged in the kit body and a plurality of light source pieces arranged on two sides of the kit body; grooves are formed in two sides of the light guide body, a plurality of liquid test channels are formed in the light guide body at intervals, the liquid test channels are distributed in parallel, an ultraviolet excitation layer is arranged on the groove wall, the ultraviolet excitation layer at the groove of the light guide body is transversely irradiated by the light source piece and can excite ultraviolet light, and the ultraviolet light penetrates through the light guide body to irradiate sample solution in the liquid test channels; the light guide body is connected with the kit in an inserting mode, and a flexible light-transmitting film is arranged on the surface of the light guide body; the end part of the light guide body is provided with a liquid outlet, the end part of the liquid outlet is connected with a temporary storage cavity, and the end part of the temporary storage cavity is connected with an air bag; a display piece is arranged on one side of the light guide body, and the display piece can display a fluorescent strip; and detecting plant pathogens by the characteristics of the fluorescent bands.
Description
Technical Field
The application relates to the field of germ testing and test strip detection, in particular to a plant germ rapid detection device.
Background
The tobacco bacterial wilt is a vascular bundle disease caused by Lawsonia of Solanaceae, is difficult to detect at the initial stage, has no effect on drug control once wilting symptoms exist, strengthens detection of undeveloped tobacco roots of the bacteria, is critical for advanced control, and researches show that the incidence of tobacco plants rises along with the increase of the quantity of bacterial wilt in the tobacco roots and the foot leaves.
The traditional detection device can only perform ultraviolet irradiation with fixed characteristics, ultraviolet excitation with different characteristics is difficult to realize, the operation is troublesome in the process of detecting plant pathogens, the existing bacterial wilt detection method mainly comprises a molecular biological detection method and a conventional detection method, and the molecular biological detection method such as quantitative PCR detection can accurately obtain the quantity of bacterial wilt pathogens, but has the defects of complex process, high technical difficulty and high requirements on operators. The conventional measuring method has the defects that the error judgment rate of a beginner with a long detection period is high, and the requirements of rapidness and accuracy are difficult to reach.
Disclosure of Invention
The application overcomes the defects of the prior art and provides a plant pathogen rapid detection device.
In order to achieve the above purpose, the application adopts the following technical scheme: a plant pathogen rapid detection device, comprising: the kit comprises a kit body, a light guide body arranged in the kit body and a plurality of light source pieces arranged on two sides of the kit body; it is characterized in that the method comprises the steps of,
grooves are formed in two sides of the light guide body, a plurality of liquid test channels are formed in the light guide body at intervals, the liquid test channels are distributed in parallel, an ultraviolet excitation layer is arranged on the groove wall, the ultraviolet excitation layer at the groove of the light guide body is transversely irradiated by the light source piece and can excite ultraviolet light, and the ultraviolet light penetrates through the light guide body to irradiate sample solution in the liquid test channels;
the light guide body is connected with the kit in an inserting mode, and a flexible light-transmitting film is arranged on the surface of the light guide body;
the end part of the light guide body is provided with a liquid outlet, the end part of the liquid outlet is connected with a temporary storage cavity, and the end part of the temporary storage cavity is connected with an air bag;
a display piece is arranged on one side of the light guide body, and the display piece can display a fluorescent strip;
and detecting plant pathogens by the characteristics of the fluorescent bands.
In a preferred embodiment of the present application, the light guide body only receives any wavelength or a range of wavelengths from 360nm to 500nm for excitation display.
In a preferred embodiment of the present application, the cross section of the test solution channel may be a V-shaped structure, a U-shaped structure, or an O-shaped structure.
In a preferred embodiment of the application, the air bag is of a flat structure, a matching piece is arranged above the air bag, an extrusion piece is arranged on the kit, the extrusion piece penetrates through the upper surface of the kit, and the bottom of the extrusion piece is matched with the matching piece.
In a preferred embodiment of the present application, the display element includes a T line and a C line.
In a preferred embodiment of the present application, when red fluorescence is displayed at both the C line and the T line, the number of bacterial wilt bacteria is equal to or higher than the detection limit.
In a preferred embodiment of the present application, when red fluorescence is not displayed at line C, indicating incorrect operation or failure of the test strip, a secondary test can be performed by replacing the test strip.
In a preferred embodiment of the present application, the ultraviolet excitation layer may be a multi-layer structure, and the ultraviolet excitation layer is at least sequentially provided with a first light emitting layer, a second light emitting layer, and a third light emitting layer.
In a preferred embodiment of the present application, the light source member is capable of irradiating spot light, line light or band light.
In a preferred embodiment of the present application, the light guide body can be a tubular hollow structure, and when there are two light guide bodies, the two light guide bodies are distributed in parallel.
In a preferred embodiment of the present application, the size of the sample channel is 1mm-5mm.
The application solves the defects existing in the background technology, and has the following beneficial effects:
1. the inside of the light guide body is provided with a plurality of test solution channels in parallel, so that the simultaneous detection of different test solutions can be realized, the differentiation of the same test solution can be detected, and the contrast is strong.
2. The light guide body is excited by ultraviolet light through transverse light irradiation, an ultraviolet flashlight with fixed wavelength is not required to be independently selected for irradiation, the detection is convenient and flexible, meanwhile, the light guide body is internally arranged in the kit, the occupied space is reduced, the detection strip is detachably connected with the kit, the light guide body is connected in a plugging mode, a buckling mode or a pasting mode can be selected, and the replacement of the light guide body is convenient.
3. The light guide body can excite ultraviolet light and circulate test liquid, so that the light guide body is integrated into a whole and has higher use flexibility.
4. Ultraviolet light with different characteristics can be excited by changing the characteristics of the light source, and detection of different plant pathogens can be performed according to the ultraviolet light with different characteristics.
Drawings
In order to more clearly illustrate the embodiments of the application or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the application, and that other embodiments of the drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic perspective view of a detection device according to the present application.
Fig. 2 is a schematic view of the structure of the light guide of the present application.
FIG. 3 is a schematic side view of the kit of the present application.
Reference numerals:
1. the kit comprises a kit body, 2, a knob, 3, a light source part, 4, a test solution channel, 5, a light guide body, 6, a liquid inlet, 7, a pressing part, 8, a C line, 9, a T line, 10, a display part, 11, a temporary storage cavity, 12, an air bag, 13, a matching part, 14, a power key, 15, an indicator light, 16 and a heat dissipation groove.
Detailed Description
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other.
In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the drawings, are merely for convenience in describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the scope of the present application. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may include one or more of the feature, either explicitly or implicitly. In the description of the application, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art in a specific case.
In order that the application may be readily understood, a more complete description of the application will be rendered by reference to the appended drawings. The drawings illustrate preferred embodiments of the application. This application may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
The application will now be described in further detail with reference to the drawings and examples, which are simplified schematic illustrations of the basic structure of the application, which are presented only by way of illustration, and thus show only the structures that are relevant to the application.
As shown in FIG. 1, the application discloses a plant pathogen rapid detection device.
It should be noted that, the plant germ rapid detection device includes: the kit comprises a kit body and a light guide body arranged in the kit body; grooves are formed in two sides of the light guide body, a plurality of test solution channels are formed in the light guide body at intervals, ultraviolet excitation layers are arranged on the groove walls, ultraviolet light can be excited by the ultraviolet excitation layers at the grooves of the light guide body when the light source piece transversely irradiates, and the ultraviolet light penetrates through the light guide body to irradiate sample solution in the test solution channels; the top of the light guide body is provided with a plurality of wires which can display the fluorescent band; plant pathogens are detected by the characteristics of the fluorescent bands.
Specifically, the light source piece can illuminate spot light, line light or wave band light, and according to the difference of plant germ kind that needs to detect, the light source characteristic that the light source piece was illuminated is different, and the kit can be dismantled with the light guide body and be connected, including grafting, buckle connection or paste connection, but not limited to these several connected modes, as long as guarantee that the light guide can be taken off in a flexible way from the kit, can select other connected modes, kit and light guide body, and the light guide body is inside the kit is inserted to at least more than half part, and the inlet exposes outside the kit, conveniently drops into the test solution through the inlet.
As shown in fig. 2, the present application discloses a schematic structure of a light guide.
The ultraviolet excitation layer can be of a multi-layer structure, and at least a first light-emitting layer, a second light-emitting layer and a third light-emitting layer are sequentially arranged on the ultraviolet excitation layer; the ultraviolet excitation layer only receives the excitation radiation of any wavelength or a certain range of wavelengths from 360nm to 500nm for display, the display wavelengths of ultraviolet excitation light of the first luminescent layer, the second luminescent layer and the third luminescent layer can be different, and the ultraviolet excitation layer can only comprise two luminescent layers which are respectively a first luminescent layer and a second luminescent layer combination, a first luminescent layer and a third luminescent layer combination and a second luminescent layer and a third luminescent layer combination;
the ultraviolet excitation layer may comprise only one light emitting layer, and may be the first light emitting layer or the second light emitting layer, or the third light emitting layer, respectively. The first luminescent layer shows green, or red, or blue by light-projected excitation radiation having a wavelength between 360nm and 400nm, and the second luminescent layer shows red, or blue, or green, or yellow, or white by light-projected excitation radiation having a wavelength between 400nm and 430 nm. The third luminescent layer shows red, or blue, or green, or yellow by projection of excitation radiation with a wavelength between 430nm and 500nm. The ultraviolet excitation layer may then display a single red, or single blue, or single green, or single yellow, or single white color upon excitation radiation of the particular wavelength projection device.
The kit one side is provided with the draw-in groove that corresponds with the leaded light body, and the mode of pegging graft the leaded light body inside the kit through pegging graft, and the test paper strip shows the fluorescence area, and the fluorescence area shows according to the colour, the luminance of ultraviolet excitation layer, can understand that the fluorescence area can show different colours, also can show different luminance contrast, is provided with the display piece on one side of the kit, is provided with C line and T line on the display piece, and C line department can show the fluorescence area with T line department, shows through the fluorescence area, can carry out the detection of plant germ.
According to the embodiment of the application, the light guide body only receives any wavelength or a certain range of wavelengths from 360nm to 500nm to excite and display.
When the number of the light guides is two, the two light guides are distributed in parallel,
according to the embodiment of the application, the section of the test solution channel can be of a V-shaped structure, a U-shaped structure or an O-shaped structure, and the test solution channel is an arc-shaped groove.
It should be noted that, the test solution channel on the test strip may have other structures, so long as the normal circulation of the test solution can be ensured, and the shape of the test solution channel 9 can be changed under the condition of uniform flow velocity.
According to the embodiment of the application, a plurality of test liquid channels are distributed in parallel.
It should be noted that the kit can be a protein detection kit, specifically can be an ELISA kit and a fluorescent kit, but is not limited to the two types of kit, and a person skilled in the art can change and adjust the types of the kit according to specific use, when the test solution flows, the test solution is distributed in parallel, so that the test solution quantity at each region of the test solution can be ensured to be uniform, the data deviation is smaller in the test solution measurement process, the parallel arrangement of the test solution channels can be ensured, the mobility of the test solution is higher, the multiple test solution channels synchronously flow, the test solution channels on the test solution can be of an arc-shaped opening structure, the surface tension of the liquid is increased in the test solution flowing process, the test solution can be completely immersed into the test solution through capillary effect, and the measurement is accurate.
According to the embodiment of the application, the light guide body is connected with the kit 1 in a plugging manner.
The kit comprises a kit body, a light guide body, a reagent box, a display line, a detection device and a detection device, wherein the reagent box body is provided with a clamping groove corresponding to the light guide body, the light guide body is inserted into the reagent box in an inserting mode, the reagent box body is provided with the display line which comprises a C line and a T line, red fluorescence is displayed at the C line in the bacterial wilt bacteria detection process, and the T line is not displayed, so that bacterial wilt bacteria data are lower than a detection limit value and are negative;
when the C line and the T line simultaneously display red fluorescence, the quantity of the bacterial wilt bacteria is equal to or higher than a detection limit value;
when the C line does not display red fluorescence, the incorrect operation process is indicated or the test strip is failed, and the test strip can be replaced for detection again;
when the C line does not display red fluorescence, the T line displays stronger fluorescence, which indicates that the quantity of the bacterial wilt bacteria in the test solution is extremely high, and the quantity of the bacterial wilt bacteria is judged by the fluorescence display characteristic of the display line.
According to the embodiment of the application, the surface of the light guide body is provided with the flexible light-transmitting film, the bottom of the light guide body is provided with the ultraviolet excitation layer, the ultraviolet excitation layer only receives the excitation of any wavelength of 360-500 nm or a wavelength light source within a certain range to display ultraviolet light, the ultraviolet excitation layer is arranged at the bottom of the light guide body again, backlight excitation can be realized, when test paper strip fluorescent display is carried out, the brightness is higher, the display is clearer, the light source piece irradiates and uses the output specific wavelength projection equipment, the end part of the light source piece is provided with the knob, the outer side of the knob is provided with threads, the brightness of the light source piece can be adjusted by rotating the knob, the distance between the light source of the light source piece and the inner wall of the light guide body groove is output, the output specific wavelength projection equipment can be projection equipment based on the DLP technical principle, the light source in the output specific wavelength projection equipment is an LED light source, and the wavelength of the LED light source is 360-500 nm. The LED light sources can be one or two or three, and the wavelength of the LED light sources in the light sources is any one wavelength or any certain small-range wavelength or any certain two small-range wavelength or any certain three-range wavelength in the range of 360nm-500nm.
The ultraviolet excitation layer can display any one color among blue, red, green, yellow and white and any two colors and more than two mixed colors, including red, green and blue full colors, when the ultraviolet excitation layer receives a light source with a specific wavelength, ultraviolet light can be excited, passes through the ultraviolet excitation layer and irradiates on a test solution, a fluorescent band can be displayed on a test strip, bacterial wilt data is judged according to the display brightness or display characteristics of the fluorescent band, and then test strip quality detection and test strip quality screening are performed through test strip detection differences of the same batch.
According to an embodiment of the application, the surface of the light guide body is provided with a flexible light-transmitting film.
Specifically, the flexible light-transmitting film on the surface of the light guide body can enable a light source with specific wavelength to pass through, the ultraviolet excitation layer is arranged at the bottom of the light guide body and only receives the excitation of a light source with the wavelength of any wavelength or a certain range of wavelengths from 360nm to 500nm to display ultraviolet light, the ultraviolet excitation layer is arranged at the bottom of the light guide body, the specific light source passes through the flexible light-transmitting film and irradiates on the ultraviolet excitation layer, so that the ultraviolet excitation layer can excite the ultraviolet light, a test solution in the light guide body is irradiated by the ultraviolet light to perform rapid detection of plant pathogens including tobacco bacterial wilt, but the plant pathogens include the bacterial, the wavelength of the light source projected by the projection equipment with the specific wavelength is regulated and output according to the characteristic of detecting the bacterial, so that the wavelength of the light source entering the flexible light-transmitting film is controlled, and the light quantity of the light source entering the flexible light-transmitting film can be controlled, namely the light throughput can be understood.
The light guide body is excited by ultraviolet light through transverse light irradiation, an ultraviolet flashlight with fixed wavelength is not required to be independently selected for irradiation, the detection is convenient and flexible, meanwhile, the light guide body is internally arranged in the kit, the occupied space is reduced, the detection strip is detachably connected with the kit, the light guide body can be conveniently replaced by adopting a plugging mode to be connected, a buckling mode or a pasting mode can be also selected, the light guide body is arranged into a groove structure on two sides, in the transverse light source irradiation process, certain diffraction and refraction effects can be generated by a light source, the light source saturation in the groove is higher through the diffraction and refraction effects of light, meanwhile, the light source is uniformly irradiated on an ultraviolet excitation layer, the ultraviolet brightness at the excitation position of the ultraviolet excitation layer is uniform, and when the ultraviolet light is irradiated on a test strip for detecting plant bacteria, the ultraviolet light is more accurate.
The light guide body is transparent structure, and the light guide body both can excite the ultraviolet ray, can carry out the circulation of examination liquid again, realizes the integrative dual-purpose of light guide body, and the flexibility of use is higher, does not need to shine through external ultraviolet flashlight, can adjust ultraviolet characteristic simultaneously according to the light source wavelength.
According to the embodiment of the application, the size of the test solution channel is 1mm-5mm.
It should be noted that the dimensions of the test solution channel include 1mm-3mm,4mm-5mm,3mm,5mm, and according to the test strip, different dimensions of the test solution channel can be set in the test strip production process, and also different dimensions of the test solution channel can be set according to the solubility of different test solutions and the difference of the surface tension of the liquid, so that the test solution flows at a constant speed.
According to the embodiment of the application, at least one liquid inlet and at least one liquid outlet are respectively arranged at two ends of the light guide body.
The method is characterized in that a test solution sample comprises a tobacco root sample solution or a leaf sample solution, when the root sample is sampled, soil of the root is completely washed away by tap water, only the root is left, the root is smashed, a buffer solution is added, stirring is carried out uniformly, the root sample solution is obtained, the sample solution is slowly titrated onto a test strip in a light guide body from a liquid inlet through a dropper, the sample solution reacts on the test strip for 10 minutes, ultraviolet light excitation is carried out through the light guide body to display fluorescence, a bacterial wilt data index is judged, then the same solution is used for detection, and the number of bacterial wilt is judged.
According to the embodiment of the application, the end part of the liquid outlet is connected with a temporary storage cavity.
It should be noted that, the liquid outlet can be discharged unnecessary test solution, and unnecessary test solution flows to the cavity of keeping in, can guarantee like this that there is new test solution circulation constantly on the test strip, improves the accuracy that detects.
According to the embodiment of the application, the temporary storage cavity can temporarily store the test solution flowing out of the light guide body.
It should be noted that, the temporary storage cavity can be one, two or more, and the test strip quality of different batches is different, has the difference, therefore the test strip of different batches all has the difference to the absorption capacity of test solution sample, and through setting up the temporary storage cavity, the test strip is soaked to the internal test solution of prevention leaded light, causes the inefficacy of test strip, consequently need arrange unnecessary test solution on the test strip to the unified processing of temporary storage cavity.
In another embodiment of the application, firstly, sample liquid samples are obtained, the sample liquid sample methods for obtaining different positions are different, the sample liquid samples comprise tobacco root sample solutions or leaf sample solutions, when the root samples are sampled, tap water is used for completely flushing soil at the root, only the root is left, the root is smashed, buffer solution is added and uniformly stirred to obtain the root sample solution, the sample solution is slowly titrated onto a test strip in a light guide body from a liquid inlet through a dropper, the sample solution reacts on the test strip for 10 minutes, ultraviolet light excitation is carried out through the light guide body to display fluorescence, and the data index of the bacterial wilt bacteria is judged.
When the light guide bodies are provided with a plurality of light guide bodies, the light guide bodies are distributed in parallel, the adjacent light guide bodies are communicated through the collecting pipes, the collecting pipes are of a hollow structure, each two light guide bodies are in a group, one ends of the two light guide bodies in the group are communicated through the collecting pipes, the communicating parts are connected with the connecting parts of the collecting pipes, the valves are arranged between the light guide bodies and the collecting pipes, the valves are sequentially opened in the process of flowing test liquid through the light guide bodies in different groups, the valves are closed after the test liquid is completely absorbed by the test strip in one group of light guide bodies, the valves in the next group are opened, the rest valves are all closed, the second type of test liquid is detected, two groups or more groups of valves are simultaneously opened, different batches of test strips are arranged in the different groups of the collecting pipes, the use mode is more flexible and various, the simultaneous detection of different test liquids can be realized, the difference of the same test strips can be detected, the difference of the same test strip is judged according to the property of the same test liquid, the test strip of the same batch has a screening effect, the comparison is strong, the detection data can be improved, the opening of the test paper can be controlled, and the opening of the test liquid can be controlled.
The test paper strip detection reference performance is poor due to the difference of test liquid samples, and meanwhile, when multiple factors such as test paper strips in different batches, test liquid sample differences and the like are interfered at the same time, the detection result is deviated, and in the comparison process of the test paper strip, the variable factors in the comparison process of the test paper strip can be reduced, other variables are controlled to be the same, only the same variable is changed, the comparison of single variable is carried out, the transverse comparison is realized, the error in the detection process is reduced, and the reference performance is higher.
According to the embodiment of the application, the air bag is of a flat structure, the matching piece is arranged above the air bag, the reagent box is provided with the extrusion piece, the extrusion piece penetrates through the upper surface of the reagent box, and the bottom of the extrusion piece is matched with the matching piece.
The liquid outlet end connection has the chamber of keeping in, the chamber of keeping in can keeping in the test liquid that the light guide body was flowed out, the chamber of keeping in can be one, two or more, consequently, need arrange unnecessary test liquid on the test paper strip to keeping in the chamber unified processing, the liquid outlet can be with unnecessary test liquid discharge, unnecessary test liquid flows to the chamber of keeping in, can guarantee to constantly have new test liquid circulation on the test paper strip like this, improve the accuracy of detection, when carrying out the test liquid circulation, the test liquid passes through side by side distribution, can guarantee that the test liquid volume of each regional department of test paper strip is more even, in carrying out the test paper strip survey in-process, the data deviation is less, the test liquid passageway sets up side by side, still can guarantee that the mobility of test liquid is stronger, and many test liquid passageway synchronous flow, the test liquid passageway on the test paper strip can be arc opening structure, in the test liquid flow in-process, increase liquid surface tension, through capillary effect, make the test liquid can fully immerse the test paper strip, the survey is accurate.
The inside of the light guide body is provided with a plurality of test solution channels in parallel, so that the simultaneous detection of different test solutions can be realized, the differentiation of the same test solution can be detected, and the contrast is strong.
The light guide body is excited by ultraviolet light through transverse light irradiation, an ultraviolet flashlight with fixed wavelength is not required to be independently selected for irradiation, the detection is convenient and flexible, meanwhile, the light guide body is internally arranged in the kit, the occupied space is reduced, the detection strip is detachably connected with the kit, the light guide body is connected in a plugging mode, a buckling mode or a pasting mode can be selected, and the replacement of the light guide body is convenient.
The light guide body can excite ultraviolet light and can circulate test liquid, so that the light guide body is integrated into a whole, the use flexibility is high, the external ultraviolet flashlight is not required to irradiate, and the ultraviolet characteristic can be adjusted according to the wavelength of the light source.
Ultraviolet light with different characteristics can be excited by changing the characteristics of the light source, and detection of different plant pathogens can be performed according to the ultraviolet light with different characteristics.
FIG. 3 shows a schematic side structural view of the kit;
the kit is provided with the power key on one side, can control the start-up or the stop of kit through the power key, and power key one side is provided with the pilot lamp, and the kit operation in-process, the pilot lamp is bright often, and the kit back is provided with a plurality of heat dissipation groove, can discharge the heat of kit operation in-process fast through the heat dissipation groove, prevents that the kit from generating heat.
It should be noted that the above-mentioned embodiments illustrate rather than limit the application, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims. In the claims, the word "comprising" does not exclude the presence of data or steps not listed in a claim.
The above-described preferred embodiments according to the present application are intended to suggest that, from the above description, various changes and modifications can be made by the person skilled in the art without departing from the scope of the technical idea of the present application. The technical scope of the present application is not limited to the description, but must be determined according to the scope of claims.
Claims (9)
1. A plant pathogen rapid detection device, comprising: the kit comprises a kit body, a light guide body arranged in the kit body and a plurality of light source pieces arranged on two sides of the kit body; it is characterized in that the method comprises the steps of,
grooves are formed in two sides of the light guide body, a plurality of liquid test channels are formed in the light guide body at intervals, the liquid test channels are distributed in parallel, an ultraviolet excitation layer is arranged on the wall of the groove, the light source piece transversely irradiates ultraviolet light excited by the ultraviolet excitation layer at the groove of the light guide body, and the ultraviolet light penetrates through the light guide body to irradiate sample solution in the liquid test channels;
the light guide body is connected with the kit in an inserting mode, a flexible light-transmitting film is arranged on the surface of the light guide body, and a test strip is arranged in the light guide body;
the end part of the light guide body is provided with a liquid outlet, the end part of the liquid outlet is connected with a temporary storage cavity, and the end part of the temporary storage cavity is connected with an air bag;
a display piece is arranged on one side of the light guide body, and the display piece displays a fluorescent strip;
detecting plant pathogens by the characteristics of the fluorescent band;
the gasbag is flat structure, the gasbag top is provided with the cooperation piece, be provided with the extrusion piece on the kit, the extrusion piece runs through the kit upper surface, the extrusion piece bottom with the cooperation piece cooperatees.
2. The rapid detection device for plant pathogens according to claim 1, wherein the light guide body only receives any wavelength or a range of wavelengths from 360nm to 500nm for excitation display.
3. The rapid plant pathogen detection device according to claim 2, wherein the cross section of the test solution channel is of a V-shaped structure or a U-shaped structure or an O-shaped structure.
4. The rapid plant pathogen detection device of claim 1, wherein the display includes a T-line and a C-line.
5. The rapid plant pathogen detection device according to claim 4, wherein red fluorescence is displayed at both the C line and the T line, indicating that the number of bacterial wilt is equal to or higher than the detection limit.
6. The rapid plant pathogen detection device according to claim 4, wherein when red fluorescence is not displayed at the line C, a secondary detection is performed by replacing the test strip, indicating incorrect operation or that the test strip has failed.
7. The rapid detection device for plant pathogens according to claim 1, wherein the ultraviolet excitation layer is of a multi-layer structure, and the ultraviolet excitation layer is provided with at least a first light-emitting layer, a second light-emitting layer and a third light-emitting layer in sequence.
8. A plant pathogen rapid detecting device according to claim 1, wherein the light source irradiates spot light, line light or band light.
9. The rapid plant pathogen detection device according to claim 1, wherein the light guide is a tubular hollow structure, and when there are two light guides, the two light guides are arranged in parallel.
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| CN202010311378.9A CN111487231B (en) | 2020-04-20 | 2020-04-20 | Plant germ rapid detection device |
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