CN111450172A - Black tea extract for inhibiting SW480 proliferation of human colorectal cancer cells and application thereof - Google Patents
Black tea extract for inhibiting SW480 proliferation of human colorectal cancer cells and application thereof Download PDFInfo
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- CN111450172A CN111450172A CN202010017308.2A CN202010017308A CN111450172A CN 111450172 A CN111450172 A CN 111450172A CN 202010017308 A CN202010017308 A CN 202010017308A CN 111450172 A CN111450172 A CN 111450172A
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- 244000269722 Thea sinensis Species 0.000 title claims abstract description 59
- 235000006468 Thea sinensis Nutrition 0.000 title claims abstract description 50
- 235000020279 black tea Nutrition 0.000 title claims abstract description 49
- 239000000284 extract Substances 0.000 title claims abstract description 42
- 206010009944 Colon cancer Diseases 0.000 title claims abstract description 24
- 208000001333 Colorectal Neoplasms Diseases 0.000 title claims abstract description 22
- 230000035755 proliferation Effects 0.000 title claims abstract description 15
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 12
- 235000013616 tea Nutrition 0.000 claims abstract description 8
- 239000000706 filtrate Substances 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 8
- 235000013305 food Nutrition 0.000 claims description 6
- 230000036541 health Effects 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 2
- 238000012545 processing Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 238000003809 water extraction Methods 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000009702 cancer cell proliferation Effects 0.000 description 2
- 208000029742 colonic neoplasm Diseases 0.000 description 2
- 238000000643 oven drying Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000001145 Metabolic Syndrome Diseases 0.000 description 1
- 201000000690 abdominal obesity-metabolic syndrome Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000397 bevacizumab Drugs 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229940046011 buccal tablet Drugs 0.000 description 1
- 239000006189 buccal tablet Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229960005395 cetuximab Drugs 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 235000020333 oolong tea Nutrition 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/22—Drying or concentrating tea extract
- A23F3/26—Drying or concentrating tea extract by lyophilisation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/30—Further treatment of dried tea extract; Preparations produced thereby, e.g. instant tea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The invention relates to a black tea extract for inhibiting the proliferation of human colorectal cancer cells SW480 and application thereof, belonging to the technical field of tea processing.
Description
Technical Field
The invention belongs to the technical field of tea processing, and particularly relates to a black tea extract for inhibiting the proliferation of human colorectal cancer cells SW480 and application thereof.
Background
Colorectal cancer is one of the common malignant tumors in the world. The incidence rate of colorectal cancer in China has increased by 3 to 4 percent in every 30 years. The morbidity and the mortality of colorectal cancer in China respectively account for 18.6 percent and 20.1 percent of the total morbidity and mortality in the world, and are in the 1 st level. According to the national tumor registration data report of the national cancer center, the colorectal cancer incidence rates of cities and rural areas of China are respectively 3 rd and 5 th of all malignant tumors, and the disease death rates of the colorectal cancer are respectively 4 th and 5 th. Although some new methods, such as cetuximab, bevacizumab and other molecular targeting drugs, emerge from the aspect of colon cancer treatment at present, the colon cancer treatment effect is still poor overall, and no satisfactory non-invasive treatment scheme exists.
Black tea is the major tea species produced and consumed in the world, and is consumed in higher amounts than green tea and oolong tea. Researches show that the black tea has various biological activities, such as antioxidation, atherosclerosis prevention, weight reduction, inflammation resistance, tumor resistance, metabolic syndrome regulation and the like. The invention takes black tea as a raw material to prepare a black tea extract capable of inhibiting the proliferation of human colorectal cancer cells SW 480. Through the literature search, the same public reports as the invention are not found.
Disclosure of Invention
The invention aims to provide a black tea extract capable of inhibiting the proliferation of human colorectal cancer cells SW480 and application thereof,
in order to realize the purpose, the invention is realized by the following technical scheme:
the black tea extract is prepared by a water extraction method, and specifically comprises the following steps of carrying out superfine grinding on black tea, extracting the ground tea for 1-60 minutes by using a proportion of boiling water to 1g to 1-200m L, filtering to obtain filtrate, repeating the extraction step for 2 times, combining the filtrates, concentrating and drying the filtrate to obtain the black tea extract.
Further preferably, the drying is vacuum freeze drying.
Further preferably, the drying is carried out by drying at 40-80 ℃.
The black tea extract is applied to products for inhibiting the proliferation of human colorectal cancer cells SW480, and the black tea extract is freeze-dried powder or dried powder.
Application of black tea extract in products for inhibiting SW480 proliferation of human colorectal cancer cells, wherein the black tea extract is obtained by the preparation method.
The black tea extract is an application in food and health care products, and the black tea extract is freeze-dried powder or dried powder.
Use of a black tea extract in food and health care products, the black tea extract being obtained by the above preparation method.
The invention has the beneficial effects that: the black tea extract is rich in tea polyphenols, tea pigment, protein and polysaccharide, can inhibit proliferation of human colorectal cancer cell SW480, and can be applied in food and health product.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, preferred embodiments of the present invention will be described in detail below to facilitate understanding of the skilled person.
[ example 1 ]
A black tea extract is prepared by water extraction method, which comprises micronizing black tea, extracting with pulverized tea at boiling water ratio of 1g to 10m L for 40 min, filtering to obtain filtrate, repeating the above extraction steps for 2 times, mixing filtrates, concentrating the filtrate under reduced pressure, and vacuum freeze drying to obtain black tea extract (lyophilized).
[ example 2 ]
A black tea extract is prepared by water extraction method, which comprises micronizing black tea, extracting with pulverized tea at boiling water ratio of 1g to 10m L for 40 min, filtering to obtain filtrate, repeating the above extraction steps for 2 times, mixing filtrates, concentrating the filtrate under reduced pressure, and oven drying the concentrated solution at 65 deg.C to obtain black tea extract (oven drying).
Carrying out the analysis
The black tea extracts obtained in examples 1 and 2 were subjected to cell test to examine the effect of the black tea extracts on the proliferation of human colorectal cancer cells SW480 by the MTT method, and the results were as follows: as can be seen from table 1, the relative survival rates of human colorectal cancer cells SW480 cultured with different concentrations of black tea extract (lyophilized or oven dried) were all decreased with the increase of the culture time, indicating that the black tea extract can inhibit the proliferation of human colorectal cancer cells SW 480. SW480 cells are cultured by black tea extracts (freeze-dried) with different concentrations, and the effect of inhibiting cancer cell proliferation is displayed only after the culture time is longer than 24 hours; SW480 cells were cultured with black tea extract (oven dried) at different concentrations for 24h to show the effect of inhibiting cancer cell proliferation. The dose effect relationship of the black tea extracts (freeze-dried or oven-dried) with different concentrations on the inhibition effect on the proliferation of human colorectal cancer cells SW480 is not obvious.
TABLE 1 Effect of Black tea extract on the relative survival of human colorectal cancer cells SW480
Note: the difference is not significant when capital letters are marked with the same shoulder in the same row (P >0.05), and the difference is significant when capital letters are marked with different shoulders in the same row (P < 0.05); the difference is not significant when the lower case letters in the same row are marked with the same shoulder mark (P >0.05), and the difference is significant when the lower case letters in the same row are marked with different shoulders (P < 0.05).
The research result shows that: the black tea extract can inhibit proliferation of human colorectal cancer cells SW480, can be directly processed into powder, capsule, buccal tablet, beverage, etc., and is convenient for use and carrying; can also be used as additive in food.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (7)
1. A black tea extract is prepared by micronizing black tea, extracting with pulverized tea at a ratio of boiling water =1 g: 1-200m L for 1-60 min, filtering to obtain filtrate, repeating the above extraction steps for 2 times, mixing filtrates, concentrating, and drying to obtain black tea extract.
2. A black tea extract according to claim 1, wherein: the drying adopts vacuum freeze drying.
3. A black tea extract according to claim 1, wherein: the drying is carried out by drying at 40-80 ℃.
4. The application of the black tea extract in products for inhibiting the SW480 proliferation of human colorectal cancer cells is characterized in that: the black tea extract is freeze-dried powder or oven-dried powder.
5. The application of the black tea extract in products for inhibiting the SW480 proliferation of human colorectal cancer cells is characterized in that: a black tea extract obtained by the production method according to any one of claims 1 to 3.
6. The application of the black tea extract in food and health care products is characterized in that: the black tea extract is freeze-dried powder or oven-dried powder.
7. The application of the black tea extract in food and health care products is characterized in that: a black tea extract obtained by the production method according to any one of claims 1 to 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010017308.2A CN111450172A (en) | 2020-01-08 | 2020-01-08 | Black tea extract for inhibiting SW480 proliferation of human colorectal cancer cells and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010017308.2A CN111450172A (en) | 2020-01-08 | 2020-01-08 | Black tea extract for inhibiting SW480 proliferation of human colorectal cancer cells and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111450172A true CN111450172A (en) | 2020-07-28 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010017308.2A Pending CN111450172A (en) | 2020-01-08 | 2020-01-08 | Black tea extract for inhibiting SW480 proliferation of human colorectal cancer cells and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111450172A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106256357A (en) * | 2016-08-10 | 2016-12-28 | 广东省农业科学院茶叶研究所 | A kind of black tea complex with antioxidation and auxiliary lipid-lowering efficacy |
-
2020
- 2020-01-08 CN CN202010017308.2A patent/CN111450172A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106256357A (en) * | 2016-08-10 | 2016-12-28 | 广东省农业科学院茶叶研究所 | A kind of black tea complex with antioxidation and auxiliary lipid-lowering efficacy |
Non-Patent Citations (3)
| Title |
|---|
| 上海信裕生物科技有限公司: "LoVo细胞说明书", 《HTTP://WWW.BIOON.COM.CN/DOC/SHOWARTICLE.ASP?NEWSID=67346》 * |
| 生物谷: "SW480细胞说明书", 《HTTP://WWW.BIOON.COM.CN/SUB/SHOWARTICLE.ASP?NEWSID=68955》 * |
| 谭晓华等: "茶叶水提取物诱导LoVo细胞凋亡的差异", 《世界华人消化杂志》 * |
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Application publication date: 20200728 |
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