CN111334550A - Method for extracting small molecule peptide from winged nut - Google Patents
Method for extracting small molecule peptide from winged nut Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 48
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 37
- 150000003384 small molecules Chemical class 0.000 title claims description 13
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 8
- 238000000746 purification Methods 0.000 claims abstract description 3
- 239000002994 raw material Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 14
- 238000001816 cooling Methods 0.000 claims description 12
- 239000012528 membrane Substances 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 10
- 101710118538 Protease Proteins 0.000 claims description 8
- 102000004142 Trypsin Human genes 0.000 claims description 8
- 108090000631 Trypsin Proteins 0.000 claims description 8
- 238000004806 packaging method and process Methods 0.000 claims description 8
- 239000012588 trypsin Substances 0.000 claims description 8
- 235000013305 food Nutrition 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 238000007781 pre-processing Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000003531 protein hydrolysate Substances 0.000 abstract description 3
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- 230000000694 effects Effects 0.000 description 13
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- 238000001694 spray drying Methods 0.000 description 12
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The invention belongs to the technical field of biology, and particularly relates to a method for extracting small molecular peptides from winged nut kernels. The invention discloses a method for extracting small molecular peptides from winged nut kernels, which comprises the following steps: pretreating the samara kernels, extracting to obtain a samara kernel extracting solution, performing enzymolysis on the samara kernel extracting solution step by step, performing decoloration and deodorization treatment, and then performing purification treatment to obtain a small molecular peptide solution. The method for preparing the winged nut small molecular peptide in the industrialized production has the advantages of simple method, low preparation cost and the like, and the protein hydrolysate with the relative molecular mass of less than 1000u accounts for more than 90 percent of the winged nut small molecular peptide produced by the method.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a method for extracting small molecular peptides from winged nut kernels.
Background
Elaeagnus angustifolia of Elaeagnaceae has a diameter of 1m, a height of 3m-4m, and a maximum height of 10 m. The fruit is dry, stone-shaped, nearly round, has 3 wing-shaped ridges and villi on the surface, and is a special gradually dangerous species in China. The elaeagnus mollis is originated from the third ancient period, is an ancient object remained after the action of the fourth-period glacier, is mainly distributed in Shanxi and Shaanxi, mostly grows on a yin slope and a semi-yin slope at the elevation of 800-1500 m, is an excellent big oil plant, is peculiar to China, and is a national secondary protection gradually-dangerous tree species.
The samara oil tree is a good woody oil plant, the seeds of which are samara and contain high-quality grease, samara oil. The samara oil is oil extracted from samara kernel by supercritical CO2 extraction technology. And peeling the winged fruit to obtain the winged nut. The samara kernel protein has balanced amino acid content, is one kind of high quality plant protein, and may be used in producing high grade protein beverage and functional food additive.
However, there are few reports on how to prepare winged nut small-molecule peptides.
Disclosure of Invention
The invention aims to provide a preparation method for industrially producing winged nut small molecular peptide, which takes winged nut as a raw material and prepares the winged nut small molecular peptide with high small molecular peptide content by enzymolysis of composite protease.
Specifically, the invention discloses a method for extracting small molecule peptides from winged nut kernels, which comprises the following steps:
pretreating the winged nut, extracting to obtain a winged nut extracting solution, carrying out enzymolysis on the winged nut extracting solution, and then purifying to obtain a small molecular peptide solution.
It is to be understood that the present invention is not limited to the above-described steps and may include other additional steps without departing from the scope of the present invention.
Preferably, the pretreatment step comprises: pulverizing the winged nut to 20-80 meshes.
In some preferred embodiments of the present invention, the pre-treating step comprises: selecting high-quality winged nut without impurities, and crushing to 20-80 meshes.
Preferably, the extracting step comprises: adding water, adjusting pH to 7-7.8, heating and increasing pressure to 80 deg.C and 0.05MPa, and stirring at constant temperature under slightly high pressure for 3-4 hr to obtain winged nut extractive solution.
In some preferred embodiments of the present invention, the extracting step comprises: extracting with dynamic micro-pressure extraction tank, adding purified water 8-10 times the weight of the raw materials, adjusting pH to 7.5, heating and pressurizing to 80 deg.C, extracting under 0.05MPa for 3-4 hr, and discharging the extractive solution and residue into soup storage tank. The equipment carries out dynamic low-temperature extraction under the stirring condition, the liquid-solid contact is sufficient, the effective ingredients of the material can be dissolved out fully, and meanwhile, the loss of the effective ingredients caused by the structural damage of the material due to high temperature and high pressure can be avoided.
Preferably, the step of enzymatic hydrolysis comprises: cooling the winged nut extract to 52-54 deg.C; adding endoprotease 0.2-0.4 wt% and trypsin 0.1-0.3 wt% for enzymolysis, and stirring regularly during enzymolysis.
Preferably, enzymolysis is carried out for 3-4h in the enzymolysis step.
In some preferred embodiments of the present invention, the enzymolysis step is performed by adding endoprotease in an amount of 0.3% by weight of the raw material and trypsin in an amount of 0.2% by weight of the raw material for 3-4 h.
In some specific implementations of the invention, the extracting solution and the material slag are discharged into a hydrolysis tank from a soup storage tank, the temperature is reduced to 52-54 ℃, endoprotease accounting for 0.3 percent of the weight of the raw material and trypsin accounting for 0.2 percent of the weight of the raw material are added for enzymolysis for 3.5 hours, and the mixture is stirred for 10 minutes every 0.5 hour in the enzymolysis process, so that the enzymolysis is uniformly and fully carried out.
Preferably, the purification treatment comprises; centrifuging to remove residues, performing membrane treatment, concentrating and separating to obtain the small molecular peptide solution.
More preferably, the centrifugal deslag step comprises: inactivating the enzymolysis liquid at high temperature, cooling to 65-70 ℃, then removing slag through a horizontal spiral sedimentation centrifuge, and completely separating material slag and liquid in the enzymolysis liquid to obtain centrifugal slag removal liquid. The horizontal spiral sedimentation centrifuge has the advantages of good adaptability, large processing capacity and good separation effect; the full-automatic operation, long service life and low energy consumption; the separation of the suspension is carried out under the completely closed condition, no pollution is caused to the operation site, and the clean and sanitary production environment can be kept.
Further, the high-temperature inactivation step of the enzymolysis liquid comprises the following steps: heating the enzymolysis solution to 85 deg.C, and maintaining for 15-20 min.
More preferably, the membrane separation step comprises: and (4) ultrafiltering the centrifugal deslagging liquid by a multifunctional inorganic ceramic membrane separation device, and filtering the filtrate to a clear liquid storage tank. The separation process does not need to add additional auxiliary agents, the materials do not change phases, the normal (low) temperature and low pressure operation is realized, the energy consumption is low, the material separation precision is high, the filtrate is clear and transparent, and the method is suitable for separating the substances with high requirements on heat sensitivity, high purity and the like.
In some embodiments of the invention, the centrifugal deslag liquid is ultrafiltered by a multifunctional inorganic ceramic membrane separation device, the filtering temperature is 50-60 ℃, and the pressure is 0.1-0.2 MPa.
More preferably, the double-effect vacuum energy-saving concentration step comprises the following steps: concentrating the filtrate with double-effect vacuum energy-saving concentrating equipment to obtain concentrated solution, wherein the first-effect vacuum degree and temperature are respectively controlled at-0.06 MPa and 60-70 deg.C, the second-effect vacuum degree and temperature are respectively controlled at-0.08 MPa and 50-60 deg.C, and the concentration temperature, vacuum degree and gas supply pressure are comprehensively controlled to obtain the final product. The concentration method can avoid the influence of high temperature on the activity of the product, and is suitable for the concentration of thermosensitive and small molecular materials. In addition, the concentration process is totally closed without foam generation, the evaporation speed is high, and the concentration ratio is heavy.
More preferably, the high-speed tubular separation step comprises: the concentrated solution is separated for the second time by a tube type separator with the rotating speed of 16000 r/min.
In some embodiments of the invention, the concentrated solution is cooled to 50-60 ℃ and then passed through a tubular separator for secondary separation at a rotation speed of 16000r/min, so as to ensure that no impurity remains in the final product.
Preferably, the method further comprises the step of concentrating and drying the obtained molecular peptide solution to obtain the winged nut peptide powder. More preferably, the winged nut peptide powder is prepared by a high-speed centrifugal spray drying method.
In some embodiments of the invention, the high speed centrifugal spray drying step comprises: the concentrated solution which achieves the sterilization effect after being heated to 85 ℃ is subjected to powder spraying and drying by a high-speed centrifugal spray drying granulator, the air inlet temperature is 170-180 ℃, and the air outlet temperature is 85-90 ℃.
Preferably, the method further comprises a finished product packaging step. In some embodiments of the invention, food grade packaging material is used, and the package is sealed.
The invention also discloses a small molecular peptide of the winged nut prepared by the method.
In a third aspect of the invention, the invention discloses a food which comprises the winged nut small molecular peptide. The food is solid or liquid.
The fourth aspect of the invention discloses the application of the method or the winged nut small molecular peptide in the fields of medicine and food. The winged nut small molecular peptide obtained by the method has great development and utilization values. Preferably, the food field comprises the field of health products.
On the basis of the common general knowledge in the field, the above-mentioned preferred conditions can be combined arbitrarily without departing from the concept and the protection scope of the invention.
Compared with the prior art, the invention has the following remarkable advantages and effects:
the invention discloses a method for industrially producing winged nut small molecular peptide, which is simple and low in preparation cost, and the protein hydrolysate with the relative molecular mass of less than 1000u accounts for more than 90 percent of the winged nut small molecular peptide produced by the method.
Drawings
FIG. 1 is a schematic view of the process flow of extracting small molecule peptides from winged nut according to the embodiment of the present invention.
Detailed Description
The technical solutions of the present invention are described in detail below with reference to the drawings and the embodiments, but the present invention is not limited to the scope of the embodiments.
The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions. The reagents and starting materials used in the present invention are commercially available.
Example 1
The embodiment discloses a method for extracting small molecule peptide from winged nut, the process flow chart of which is shown in figure 1, and the method specifically comprises the following steps:
(1) pretreatment of raw materials: selecting high-quality winged nut without impurities, and crushing to 20-80 meshes.
(2) Extraction: extracting with dynamic micro-pressure extraction tank, adding purified water 8-10 times the weight of the raw materials, adjusting pH to 7.5, heating and pressurizing to 80 deg.C, extracting under 0.05MPa for 3-4 hr under stirring at constant temperature and under micro-pressure, and discharging the extractive solution and residue into soup storage tank. The equipment carries out dynamic low-temperature extraction under the stirring condition, the liquid-solid contact is sufficient, the effective ingredients of the material can be dissolved out fully, and meanwhile, the loss of the effective ingredients caused by the structural damage of the material due to high temperature and high pressure can be avoided.
(3) Hydrolysis: discharging the extracting solution and the material residues into a hydrolysis tank from a soup storage tank, cooling to 52-54 ℃, adding endoprotease accounting for 0.3 percent of the weight of the raw materials and trypsin accounting for 0.2 percent of the weight of the raw materials for enzymolysis for 3.5 hours, and stirring for 10 minutes every 0.5 hour in the enzymolysis process to ensure that the enzymolysis is uniformly and fully carried out.
(4) Centrifugal deslagging: heating the enzymolysis solution to 85 deg.C, maintaining for 15-20min, cooling the feed liquid to 65-70 deg.C after enzyme deactivation, removing residue by horizontal spiral sedimentation centrifuge, and completely separating residue and liquid in the enzymolysis solution. The equipment has good adaptability, large processing capacity and good separation effect; the full-automatic operation, long service life and low energy consumption; the separation of the suspension is carried out under the completely closed condition, no pollution is caused to the operation site, and the cleanness and the sanitation of the production environment can be kept.
(5) Membrane separation: the centrifugal deslagging liquid is ultrafiltered by a multifunctional inorganic ceramic membrane separation device, the filtering temperature is 50-60 ℃, and the pressure is 0.1-0.2 MPa. Filtering the filtrate to a clear solution storage tank. In the separation process, no additional auxiliary agent is needed, the materials do not change phase, the normal (low) temperature and low pressure operation is realized, the energy consumption is low, the material separation precision is high, the filtrate is clear and transparent, and the method is suitable for separating the substances with high requirements on heat sensitivity, high purity and the like.
(6) Double-effect vacuum energy-saving concentration: the filtrate is concentrated by double-effect vacuum energy-saving concentration equipment, the first-effect vacuum degree and the temperature are respectively controlled to be-0.06 MPa and 60-70 ℃, the second-effect vacuum degree and the temperature are respectively controlled to be-0.08 MPa and 50-60 ℃, the concentration temperature, the vacuum degree and the air supply pressure are comprehensively controlled by taking the content of soluble solids as indexes. The boiling point of the liquid can be reduced under the vacuum condition, the feed liquid can be concentrated at low temperature, the influence of high temperature on the activity of the product is avoided, and the method is suitable for concentrating thermosensitive and small molecular materials. In addition, the concentration process is totally closed without foam generation, the evaporation speed is high, and the concentration ratio is heavy.
(7) High-speed tubular separation: cooling the concentrated solution to 50-60 deg.C, separating with tubular separator at 16000r/min for the second time to ensure no impurity residue in the final product.
(8) High-speed centrifugal spray drying: the concentrated solution which achieves the sterilization effect after being heated to 85 ℃ is subjected to spray drying by a high-speed centrifugal spray drying granulator, and the air inlet temperature is set as follows: 170 ℃ and 180 ℃, air outlet temperature: 85-90 ℃.
(9) Packaging a finished product: and (4) adopting food-grade packaging materials, and sealing and packaging.
Example 2
The embodiment discloses a method for extracting small molecule peptide from winged nut, which specifically comprises the following steps:
(1) pretreatment of raw materials: selecting high-quality winged nut without impurities, and crushing to 20-80 meshes.
(2) Extraction: extracting with dynamic micro-pressure extraction tank, adding purified water 8-10 times the weight of the raw materials, adjusting pH to 7.5, heating and pressurizing to 80 deg.C, extracting under 0.05MPa for 3-4 hr under stirring at constant temperature and under micro-pressure, and discharging the extractive solution and residue into soup storage tank. The equipment carries out dynamic low-temperature extraction under the stirring condition, the liquid-solid contact is sufficient, the effective ingredients of the material can be dissolved out fully, and meanwhile, the loss of the effective ingredients caused by the structural damage of the material due to high temperature and high pressure can be avoided.
(3) Hydrolysis: discharging the extracting solution and the material residues into a hydrolysis tank from a soup storage tank, cooling to 52-54 ℃, adding endoprotease accounting for 0.2 percent of the weight of the raw materials and trypsin accounting for 0.1 percent of the weight of the raw materials for enzymolysis for 3.5 hours, and stirring for 10 minutes every 0.5 hour in the enzymolysis process to ensure that the enzymolysis is uniformly and fully carried out.
(4) Centrifugal deslagging: heating the enzymolysis solution to 85 deg.C, maintaining for 15-20min, cooling the feed liquid to 65-70 deg.C after enzyme deactivation, removing residue by horizontal spiral sedimentation centrifuge, and completely separating residue and liquid in the enzymolysis solution. The equipment has good adaptability, large processing capacity and good separation effect; the full-automatic operation, long service life and low energy consumption; the separation of the suspension is carried out under the completely closed condition, no pollution is caused to the operation site, and the cleanness and the sanitation of the production environment can be kept.
(5) Membrane separation: the centrifugal deslagging liquid is ultrafiltered by a multifunctional inorganic ceramic membrane separation device, the filtering temperature is 50-60 ℃, and the pressure is 0.1-0.2 MPa. Filtering the filtrate to a clear solution storage tank. In the separation process, no additional auxiliary agent is needed, the materials do not change phase, the normal (low) temperature and low pressure operation is realized, the energy consumption is low, the material separation precision is high, the filtrate is clear and transparent, and the method is suitable for separating the substances with high requirements on heat sensitivity, high purity and the like.
(6) Double-effect vacuum energy-saving concentration: the filtrate is concentrated by double-effect vacuum energy-saving concentration equipment, the first-effect vacuum degree and the temperature are respectively controlled to be-0.06 MPa and 60-70 ℃, the second-effect vacuum degree and the temperature are respectively controlled to be-0.08 MPa and 50-60 ℃, the concentration temperature, the vacuum degree and the air supply pressure are comprehensively controlled by taking the content of soluble solids as indexes. The boiling point of the liquid can be reduced under the vacuum condition, the feed liquid can be concentrated at low temperature, the influence of high temperature on the activity of the product is avoided, and the method is suitable for concentrating thermosensitive and small molecular materials. In addition, the concentration process is totally closed without foam generation, the evaporation speed is high, and the concentration ratio is heavy.
(7) High-speed tubular separation: cooling the concentrated solution to 50-60 deg.C, separating with tubular separator at 16000r/min for the second time to ensure no impurity residue in the final product.
(8) High-speed centrifugal spray drying: the concentrated solution which achieves the sterilization effect after being heated to 85 ℃ is subjected to spray drying by a high-speed centrifugal spray drying granulator, and the air inlet temperature is set as follows: 170 ℃ and 180 ℃, air outlet temperature: 85-90 ℃.
(9) Packaging a finished product: and (4) adopting food-grade packaging materials, and sealing and packaging.
Example 3
The embodiment discloses a method for extracting small molecule peptide from winged nut, which specifically comprises the following steps:
(1) pretreatment of raw materials: selecting high-quality winged nut without impurities, and crushing to 20-80 meshes.
(2) Extraction: extracting with dynamic micro-pressure extraction tank, adding purified water 8-10 times the weight of the raw materials, adjusting pH to 7.5, heating and pressurizing to 80 deg.C, extracting under 0.05MPa for 3-4 hr under stirring at constant temperature and under micro-pressure, and discharging the extractive solution and residue into soup storage tank. The equipment carries out dynamic low-temperature extraction under the stirring condition, the liquid-solid contact is sufficient, the effective ingredients of the material can be dissolved out fully, and meanwhile, the loss of the effective ingredients caused by the structural damage of the material due to high temperature and high pressure can be avoided.
(3) Hydrolysis: discharging the extracting solution and the material residues into a hydrolysis tank from a soup storage tank, cooling to 52-54 ℃, adding endoprotease accounting for 0.4 percent of the weight of the raw materials and trypsin accounting for 0.3 percent of the weight of the raw materials for enzymolysis for 3.5 hours, and stirring for 10 minutes every 0.5 hour in the enzymolysis process to ensure that the enzymolysis is uniformly and fully carried out.
(4) Centrifugal deslagging: heating the enzymolysis solution to 85 deg.C, maintaining for 15-20min, cooling the feed liquid to 65-70 deg.C after enzyme deactivation, removing residue by horizontal spiral sedimentation centrifuge, and completely separating residue and liquid in the enzymolysis solution. The equipment has good adaptability, large processing capacity and good separation effect; the full-automatic operation, long service life and low energy consumption; the separation of the suspension is carried out under the completely closed condition, no pollution is caused to the operation site, and the cleanness and the sanitation of the production environment can be kept.
(5) Membrane separation: the centrifugal deslagging liquid is ultrafiltered by a multifunctional inorganic ceramic membrane separation device, the filtering temperature is 50-60 ℃, and the pressure is 0.1-0.2 MPa. Filtering the filtrate to a clear solution storage tank. In the separation process, no additional auxiliary agent is needed, the materials do not change phase, the normal (low) temperature and low pressure operation is realized, the energy consumption is low, the material separation precision is high, the filtrate is clear and transparent, and the method is suitable for separating the substances with high requirements on heat sensitivity, high purity and the like.
(6) Double-effect vacuum energy-saving concentration: the filtrate is concentrated by double-effect vacuum energy-saving concentration equipment, the first-effect vacuum degree and the temperature are respectively controlled to be-0.06 MPa and 60-70 ℃, the second-effect vacuum degree and the temperature are respectively controlled to be-0.08 MPa and 50-60 ℃, the concentration temperature, the vacuum degree and the air supply pressure are comprehensively controlled by taking the content of soluble solids as indexes. The boiling point of the liquid can be reduced under the vacuum condition, the feed liquid can be concentrated at low temperature, the influence of high temperature on the activity of the product is avoided, and the method is suitable for concentrating thermosensitive and small molecular materials. In addition, the concentration process is totally closed without foam generation, the evaporation speed is high, and the concentration ratio is heavy.
(7) High-speed tubular separation: cooling the concentrated solution to 50-60 deg.C, separating with tubular separator at 16000r/min for the second time to ensure no impurity residue in the final product.
(8) High-speed centrifugal spray drying: the concentrated solution which achieves the sterilization effect after being heated to 85 ℃ is subjected to spray drying by a high-speed centrifugal spray drying granulator, and the air inlet temperature is set as follows: 170 ℃ and 180 ℃, air outlet temperature: 85-90 ℃.
(9) Packaging a finished product: and (4) adopting food-grade packaging materials, and sealing and packaging.
Example 4
Analyzing and detecting the winged nut peptide powder obtained in the example 1 by adopting a conventional high-efficiency gel filtration chromatography, analyzing by adopting GPC software, processing a chromatogram and data thereof, and calculating to obtain 90.42 percent of protein hydrolysate with the relative molecular mass of less than 1000u in the winged nut peptide powder.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A method for extracting small molecule peptides from winged nut kernels is characterized by comprising the following steps: pretreating the winged nut, extracting to obtain a winged nut extracting solution, carrying out enzymolysis on the winged nut extracting solution, and then purifying to obtain a small molecular peptide solution.
2. The method of claim 1, wherein the pre-processing step comprises: pulverizing the winged nut to 20-80 meshes.
3. The method of claim 1, wherein the extracting step comprises: adding water, adjusting pH to 7-7.8, heating and increasing pressure to 80 deg.C and 0.05MPa, and stirring at constant temperature under slightly high pressure for 3-4 hr to obtain winged nut extractive solution.
4. The method of claim 1, wherein the enzymatic step comprises: cooling the winged nut extract to 52-54 deg.C; adding endoprotease 0.2-0.4 wt% and trypsin 0.1-0.3 wt% for enzymolysis, and stirring regularly during enzymolysis.
5. The method according to claim 4, wherein the enzymolysis step is carried out by adding endoprotease accounting for 0.3 percent of the weight of the raw materials and trypsin accounting for 0.2 percent of the weight of the raw materials for enzymolysis for 3-4 h.
6. The method of claim 1, wherein the purification process comprises, in order; centrifuging to remove residue, performing membrane treatment, concentrating and separating to obtain winged nut small molecule peptide solution.
7. The method of claim 6, further comprising the step of concentrating and drying the resulting winged nut small molecule peptide solution to obtain winged nut peptide powder.
8. The method of claim 7, further comprising a finished product packaging step.
9. Winged nut small molecule peptides produced by the method of any one of claims 1-8.
10. A food product comprising the winged nut small molecule peptide of claim 9.
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| CN202010220154.7A CN111334550A (en) | 2020-03-25 | 2020-03-25 | Method for extracting small molecule peptide from winged nut |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117551174A (en) * | 2023-08-14 | 2024-02-13 | 北京康美禾源健康科技有限公司 | Polypeptide derived from samara seed kernel oil meal and auxiliary anti-inflammatory and antioxidant blood lipid reducing composition |
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| CN107460224A (en) * | 2017-09-28 | 2017-12-12 | 山西琪尔康翅果生物制品有限公司 | A kind of preparation method of samara oligopeptide |
| CN110777183A (en) * | 2019-12-04 | 2020-02-11 | 山西原生肽科技有限公司 | Fructus cannabis oligopeptide powder and preparation method and application thereof |
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| CN107460224A (en) * | 2017-09-28 | 2017-12-12 | 山西琪尔康翅果生物制品有限公司 | A kind of preparation method of samara oligopeptide |
| CN110777183A (en) * | 2019-12-04 | 2020-02-11 | 山西原生肽科技有限公司 | Fructus cannabis oligopeptide powder and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117551174A (en) * | 2023-08-14 | 2024-02-13 | 北京康美禾源健康科技有限公司 | Polypeptide derived from samara seed kernel oil meal and auxiliary anti-inflammatory and antioxidant blood lipid reducing composition |
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