CN111302853A - Microbial phosphorus-potassium fertilizer and preparation method thereof - Google Patents
Microbial phosphorus-potassium fertilizer and preparation method thereof Download PDFInfo
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- CN111302853A CN111302853A CN202010094528.5A CN202010094528A CN111302853A CN 111302853 A CN111302853 A CN 111302853A CN 202010094528 A CN202010094528 A CN 202010094528A CN 111302853 A CN111302853 A CN 111302853A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention relates to a microbial phosphorus-potassium fertilizer and a preparation method thereof, wherein the fertilizer is prepared from organic substances and P2O5KCL is a life carrier of microorganism WH flora, the WH flora consisting of WH2 strain, WH4 strain and WH23 strain has the functions of dissolving phosphorus and potassium, so that P, K cannot become insoluble salt after being applied to soil and is directly absorbed by crops, the quality and the seed setting rate of the crops are improved, nitrogen metabolism is promoted, excessive growth is controlled, lodging is resisted, and the soil structure is improved.
Description
Technical Field
The invention relates to the technical field of microbial fertilizers, in particular to a microbial phosphorus-potassium fertilizer and a manufacturing method thereof.
Background
For a long time, inorganic nitrogen fertilizers are singly applied to crops in vast rural areas, organic matters, phosphorus-potassium fertilizers and microorganisms are neglected to be mutually matched for use, only 1-2% of phosphorus elements in soil can be absorbed by plants, and 98% of phosphorus content exists in non-soluble mineral substances and cannot be absorbed by the plants. After the inorganic phosphorus is applied to the soil, only 10-25% of the inorganic phosphorus is generally absorbed by plants, and most of the rest of the inorganic phosphorus is combined with Fe and AL elements in the soil to form insoluble inorganic salt, so that soil hardening, fertility decline, serious damage to the soil structure and influence on the normal growth of crops are often caused.
Disclosure of Invention
The invention aims to provide a microbial phosphorus-potassium fertilizer and a manufacturing method thereof, wherein WH flora contained in the microbial phosphorus-potassium fertilizer is a phosphate-solubilizing and potassium-solubilizing microorganism, and phosphorus and potassium elements in soil can be easily absorbed by plants.
The technical solution proposed by the present invention is as follows: a microorganism for preparing phosphorus-potassium fertilizer comprises Bacillus Subtilis, China general microbiological culture Collection center (CGMCC), wherein the preservation date is 23.04.1999, the preservation number is CGMCC NO.0395.2, and the name is WH 2; bacillus Licheniformis, the preservation unit is China general microbiological culture Collection center, CGMCC for short, the preservation date is 23.04.1999, the preservation number is CGMCC NO.0395.4, and the name is WH4 strain; the Bacillus Megaterium has a preservation unit of China general microbiological culture Collection center (CGMCC), is called CGMCC for short, has a preservation date of 2019, 11 and 07 months and a preservation number of CGMCC No.18921, and is named as WH23 strain.
The microbial phosphorus-potassium fertilizer mainly comprises WH2 strain, WH4 strain, WH23 strain, calcium superphosphate, potassium chloride and rice bran.
The microbial phosphorus-potassium fertilizer contains the following strains in percentage by weight:
(1) WH2 strain: 15-25%;
(2) WH4 strain: 15-25%;
(3) WH23 strain: 50-70%.
The invention relates to a method for preparing a microbial phosphorus-potassium fertilizer, which comprises the steps of strain culture and preparation, and the method comprises the following process steps:
(1) the WH2 strain, the WH4 strain and the WH23 strain are uniformly mixed according to the weight percentage to form a WH flora;
(2) the WH flora secondary culture medium comprises the following components in percentage by weight:
80% of rice bran;
16% of soybean meal;
2% of milk powder;
1% of sugar;
the rest water;
(3) and (3) secondary strain culture: firstly, mixing and uniformly stirring a WH flora secondary culture medium according to the formula, inoculating the WH flora at normal temperature, culturing the secondary culture medium at the temperature of 35-60 ℃, at the pH value of 6.8-7.2 for 7-12 days, and obtaining active bran bacteria of the WH flora after the secondary culture medium is cultured to be mature, wherein the active bran bacteria contain 10 hundred million-20 hundred million viable bacteria per gram.
(4) Mixing and crushing calcium superphosphate, potassium chloride and active bran bacteria of WH flora according to the following weight percentage formula, and packaging to prepare the microbial phosphorus-potassium fertilizer:
40-70% of calcium superphosphate;
20-40% of potassium chloride;
10-20% of active bran fungus.
The invention relates to a biological active phosphorus-potassium compound fertilizer which is developed by utilizing a biological engineering technology, and by utilizing the strong absorption effect of a WH flora on phosphorus and potassium elements, after the fertilizer is applied to soil, phosphorus ions are not fixed by Fe and Al elements in the soil and can be directly absorbed by crops, and potassium ions can not become insoluble mineral salts and can be directly absorbed by the crops. Therefore, the seed setting rate of crops can be improved, the soil quality is improved, the nitrogen metabolism is promoted, the overgrowth and the lodging resistance are controlled, the nitrate content in the crops is increased after a large amount of nitrogen fertilizer is absorbed by the plants in the soil, the phosphorus is easily absorbed by the crops after the biological active phosphorus-potassium fertilizer is applied, the phosphorus content in the crops is increased, the nitrate content in the crops can be reduced, and the nitrate content in the crops is greatly reduced.
Compared with the prior art, the invention has the following remarkable effects:
(1) by applying the active microbial phosphorus-potassium fertilizer, under the action of WH flora, phosphorus and potassium applied in soil can not become insoluble salt and can be directly absorbed by crops.
(2) The WH flora can decompose insoluble salt in soil, improve the structure of the soil, solve the problem of phosphorus and potassium deficiency of the soil for a long time and greatly improve the soil hardening phenomenon.
(3) The nitrate content of the vegetables can be reduced by applying the active microbial phosphorus-potassium fertilizer, and the fertilizer is beneficial to human health.
The name, address, date and number of the biological material sample preservation unit and the classification name of the biological material are as follows:
Detailed Description
The invention is illustrated in further detail by the following examples.
Example 1
A microbial P-K fertilizer contains WH2 strain, WH4 strain, WH23 strain, calcium superphosphate, potassium chloride, and rice bran as main components.
Among the microorganisms, the WH2 strain is Bacillus Subtilis (Bacillus Subtilis) which is preserved in CGMCC with the preservation number of 0395.2; the WH4 strain is Bacillus Licheniformis (Bacillus Licheniformis) preserved in CGMCC with the preservation number of 0395.4; the WH23 strain is Bacillus Megaterium (CGMCC), and has a collection number of 18921.
The microbial phosphorus-potassium fertilizer contains the following strains in percentage by weight:
(1) WH2 strain: 15 percent;
(2) WH4 strain: 15 percent;
(3) WH23 strain: 70 percent.
The preparation method of the microbial phosphorus-potassium fertilizer comprises the steps of strain culture and preparation, and the process steps are as follows:
(1) the WH2 strain, the WH4 strain and the WH23 strain are uniformly mixed according to the weight percentage to form a WH flora;
(2) the formula (weight percentage) of the WH flora secondary culture medium is as follows:
80% of rice bran;
16% of soybean meal;
2% of milk powder;
1% of sugar;
the rest water;
(3) and (3) secondary strain culture: mixing and stirring a WH flora secondary culture medium uniformly according to the formula, inoculating the WH flora at normal temperature, culturing for 7 days at the temperature of 60 ℃, and obtaining active bran bacteria of the WH flora after the culture is mature, wherein the active bran bacteria contain 20 hundred million viable bacteria per gram;
(4) mixing and crushing calcium superphosphate, potassium chloride and active bran bacteria according to the following formula, and packaging to prepare the microbial phosphorus-potassium fertilizer:
40% of calcium superphosphate;
40% of potassium chloride;
20% of active bran fungus.
Example 2
The difference between the proportions and the parameters of the components of the microbial phosphorus-potassium fertilizer of the embodiment and the embodiment 1 is as follows:
1. the microbial phosphorus-potassium fertilizer contains the following strains in percentage by weight:
(1) WH2 strain: 20 percent;
(2) WH4 strain: 20 percent;
(3) WH23 strain: 60 percent.
2. The temperature of the secondary strain culture medium is 45 ℃, the pH value is 6.8, the culture time is 9 days, and the active bran bacteria of the WH flora are obtained after the culture is mature, and the number of the active bran bacteria can reach 15 hundred million/g.
3. Mixing and crushing calcium superphosphate, potassium chloride and active bran bacteria according to the following formula, and packaging to prepare the microbial phosphorus-potassium fertilizer:
55% of calcium superphosphate;
30% of potassium chloride;
15% of active bran fungus;
the proportions, parameters and process steps of the other components are the same as in example 1.
Example 3
The difference between the proportions and the parameters of the components of the microbial phosphorus-potassium fertilizer of the embodiment and the embodiment 1 is as follows:
1. the microbial phosphorus-potassium fertilizer contains the following strains in percentage by weight:
(1) WH2 strain: 25 percent;
(2) WH4 strain: 25 percent;
(3) WH23 strain: 50 percent.
2. The temperature of the secondary strain culture medium is 35 ℃, the pH value is 7.2, the culture time is 12 days, and the active bran bacteria of the WH flora are obtained after the culture is mature, and the number of the active bran bacteria can reach 10 hundred million/g.
3. The calcium superphosphate, the potassium chloride and the active bran bacteria are subjected to mud mixing and crushing according to the following formula, and are packaged to prepare the microbial phosphorus-potassium fertilizer:
70 percent of calcium superphosphate
20 percent of potassium chloride
10% of active bran fungus.
The proportions, parameters and process steps of the other components are the same as in example 1.
Example 4: application of one of the comparative examples
The organic compound fertilizer and the microbial phosphorus-potassium fertilizer (L.P.K biological fertilizer for short) are respectively applied to longan fruit trees, the organic compound fertilizer has no yield increasing effect, the microbial phosphorus-potassium fertilizer (L.P.K biological fertilizer) has obvious yield increasing effect, a plurality of fruits and uniform fruit grains, and overcomes the phenomena of large and fine fruit bearing, and the fruits are bright and lustrous. The test results are detailed in the attached table I.
Watch 1
Example 5: application of comparative example two
The organic compound fertilizer and the microbial phosphorus-potassium fertilizer (L.P.K biological fertilizer for short) are respectively applied to litchi trees, and have yield increase of different degrees, and from data analysis and field comparison, the microbial phosphorus-potassium fertilizer (L.P.K biological fertilizer) has good effect, high fruit setting rate, bright fruit skin and yield increase of 7.73 percent. The test results are detailed in table two.
Watch two
Example 6: the effect of l.p.k bio-fertilizer on rice panicles, see table three.
Watch III
| Item processing | Mu Ke count (Wan) | Mu ear number (ten thousand) | Grain number per ear | Number of ear solid | Setting percentage% | Thousand seed weight g | Yield per mu of dry grain | Increase the yield by several kg | Increased yield% |
| L.P.K biological fertilizer | 15000 | 25.6 | 151 | 142 | 93.7 | 15.2 | 387 | 48 | 14.16 |
| Blank space | 26.76 | 150 | 121.1 | 81.1 | 14.8 | 339 |
As can be seen from the above table three, although the number of the plants to be planted in the l.p.k bio-fertilizer is 1800 ten thousand less than that in the control area, the effective number of ears per mu is reduced by 1.16 ten thousand, but the number of seeds per ear is 20.9 more than that in the control area, the maturing rate is improved by 12.6%, the dry weight is increased by 0.4 g, and the yield is increased by 14.16%, which indicates that the number of seeds per ear is increased after the l.p.k bio-fertilizer is applied to the rice at the later stage, the maturing rate is improved, the weight of the seeds is increased, and the yield is increased.
Claims (4)
1. A microorganism for producing a phosphorus-potassium fertilizer, which is characterized in that: the microorganism comprises bacillus subtilis with the preservation number of CGMCC NO.0395.2 and the name of WH2 strain; the bacillus licheniformis has the preservation number of CGMCC NO.0395.4 and is named as WH4 strain; the bacillus megaterium has the preservation number of CGMCC NO.18921 and the name of WH23 strain.
2. A microbial phosphorus potassium fertilizer using the microorganism according to claim 1, characterized in that: its main components are WH2 strain, WH4 strain, WH23 strain, calcium superphosphate, potassium chloride and rice bran.
3. The microbial phosphorus-potassium fertilizer of claim 2, wherein: the microbial phosphorus-potassium fertilizer contains the following strains in percentage by weight:
(1) WH2 strain: 15-25%;
(2) WH4 strain: 15-25%;
(3) WH23 strain: 50-70%.
4. A method for preparing the microbial phosphorus-potassium fertilizer according to claim 2, which comprises strain culture and preparation, and is characterized in that:
(1) the WH2 strain, the WH4 strain and the WH23 strain are mixed evenly according to the weight percentage of claim 3 to form a WH flora:
(2) the WH flora secondary culture medium comprises the following components in percentage by weight:
80% of rice bran;
16% of soybean meal;
2% of milk powder;
1% of sugar;
the rest water;
(3) and (3) secondary strain culture: firstly, mixing and uniformly stirring a WH flora secondary culture medium according to the formula, inoculating the WH flora at normal temperature, culturing the secondary culture medium at the temperature of 35-60 ℃, the pH value of 6.8-7.2 for 7-12 days, and obtaining active bran bacteria of the WH flora after the culture is mature, wherein the active bran bacteria contain 10 hundred million-20 hundred million viable bacteria per gram;
(4) mixing and crushing calcium superphosphate, potassium chloride and active bran bacteria of WH flora according to the following weight percentage formula, and packaging to prepare the microbial phosphorus-potassium fertilizer:
40-70% of calcium superphosphate;
20-40% of potassium chloride;
10-20% of WH flora active bran bacteria.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010094528.5A CN111302853A (en) | 2020-02-16 | 2020-02-16 | Microbial phosphorus-potassium fertilizer and preparation method thereof |
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| CN202010094528.5A CN111302853A (en) | 2020-02-16 | 2020-02-16 | Microbial phosphorus-potassium fertilizer and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112280710A (en) * | 2020-10-27 | 2021-01-29 | 佛山金葵子植物营养有限公司 | Microbial fertilizer suitable for coastal saline-alkali soil sea rice and preparation method thereof |
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| CN107794240A (en) * | 2017-12-05 | 2018-03-13 | 金正大生态工程集团股份有限公司 | One plant of bacillus megaterium, its polypeptide agricultural microbial agent and preparation method and application |
| CN110452841A (en) * | 2019-07-30 | 2019-11-15 | 金葵子环保生物科技(国际)有限公司 | A kind of biological agent and its manufacturing method of rotting straws of crops |
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2020
- 2020-02-16 CN CN202010094528.5A patent/CN111302853A/en not_active Withdrawn
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| CN1255471A (en) * | 1999-11-12 | 2000-06-07 | 高明市绿宝植物营养制品有限公司 | Microbe-P-K fertilizer and its preparing process |
| CN1367243A (en) * | 2002-02-09 | 2002-09-04 | 高明金葵子植物营养有限公司 | Microbial silicon magnesium calcium fertilizer and its production method |
| CN101225367A (en) * | 2007-11-26 | 2008-07-23 | 佛山金葵子植物营养有限公司 | Microorganism, microorganism phosphatic fertilizer manufactured thereby and manufacturing method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112280710A (en) * | 2020-10-27 | 2021-01-29 | 佛山金葵子植物营养有限公司 | Microbial fertilizer suitable for coastal saline-alkali soil sea rice and preparation method thereof |
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Application publication date: 20200619 |