CN111235081B - 一种生物合成牛心果碱的微生物及其方法 - Google Patents
一种生物合成牛心果碱的微生物及其方法 Download PDFInfo
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Abstract
本发明公开了一种生物合成牛心果碱的微生物及其方法,所述微生物不含有或者敲除dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB基因,高表达或者导入aroGfbr、tyrAfb、KDC、HpaB、HpaC、DODC、CAR、sfp、aldH、CNS、4OMT、6OMT和CNMT基因。本发明改造的微生物可实现由单糖或甘油到牛心果碱的微生物转化,为大规模的生物合成牛心果碱提供了一个可行道路。
Description
技术领域
本发明属于微生物代谢工程领域,具体涉及改造微生物代谢途径,从单糖或甘油直接合成牛心果碱的方法。
背景技术
苄基异喹啉类生物碱包含2500多个种类,许多苄基异喹啉类生物碱,如:***、小檗碱和诺斯卡品等在医学领域具有广阔的应用前景与经济地位。牛心果碱是苄基异喹啉类生物碱的共同中间体。
目前已商品化的苄基异喹啉类生物碱主要通过植物提取。由于植物生长周期长及苄基异喹啉类生物碱含量低,利用植物提取苄基异喹啉类生物碱成本较高。通过生物技术手段,实现微生物发酵单糖或甘油合成苄基异喹啉类生物碱共同中间体牛心果碱,可有效的降低成本,为规模化生物合成苄基异喹啉类生物碱提供了可行的道路。
发明内容
本发明的目的在于提供一种微生物体内合成牛心果碱的方法,在微生物内构建一条由单糖或甘油转化到牛心果碱的生物合成途径。
为实现上述目的,本发明提供了在大肠杆菌合成牛心果碱的方法,生物合成路径如图1所示,主要步骤为:
(1)敲除基因构建菌株DY
以大肠杆菌MG1655菌株出发,依次敲除该菌株中七个醛还原酶基因(dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB),敲除上述7个基因的菌株命名为DY。
(2)构建产牛心果碱工程菌DY1
将来自大肠杆菌的解反馈抑制aroGfbr(3-deoxy-D-arabino heptulosonate-7-phosphate synthetase,aroG)基因和tyrAfbr(chorismate mutase/prephenatedehydrogenase,tyrA)基因及来自酿酒酵母脱羧酶KDC基因,重组到载体pBBR1MCS1上,得到载体pDY001。将来自大肠杆菌的HpaBC(4-hydroxyphenylacetate 3-hydroxylase,HpaBC,由HpaB和HpaC组成)及经密码子优化人工合成的Pseudomonas putida菌DODC(DOPAdecarboxylase,DODC)、Papaver somniferum的CNS((S)-norcoclaurine synthase,CNS)、Papaver somniferum的6OMT((RS)-norcoclaurine 6-O-methyltransferase,6OMT)、Coptis japonica的4OMT(3'-hydroxy-N-methylcoclaurine4'-O-methyltransferase,4OMT)和Papaver somniferum的CNMT((S)-coclaurine N-methyltransferase,CNMT)基因,重组到载体pET28a(+)上,得到载体pDY002。将经密码子优化人工合成的Mycobacteriummarinum菌CAR(carboxylic acid reductase,CAR)、Bacillus subtilis subsp.subtilisstr.168菌的sfp(phosphopantetheinyl transferase,sfp)和大肠杆菌MG1655的aldH(aldehyde dehydrogenase,aldH)基因重组到载体pET28b-pSC101,得到载体pDY003。将载体pDY001、pDY002和pDY003转入到敲除七个醛还原酶基因的大肠杆菌DY中,实现上述12个基因在大肠杆菌中的高量表达,得到大肠杆菌工程菌株DY2。敲除七个醛还原酶基因用于阻断中间体4-羟基苯乙醛和3,4-二羟基苯乙醛被还原为副产物4-羟基苯乙醇和3,4-二羟基苯乙醇。表达解除反馈抑制的aroGfbr基因和tyrAfb基因用于加强大肠杆菌胞内对羟基苯丙酮酸和酪氨酸的合成。表达HpaB、HpaC、KDC、aldH和CAR用于催化对羟基苯丙酮酸转化为3,4二羟基苯乙醛。表达sfp用于活化CAR酶。表达HpaB、HpaC和DODC用于催化酪氨酸转化为3,4二羟基苯乙胺,表达CNS、6OMT、4OMT和CNMT用于随后催化3,4二羟基苯乙醛与3,4二羟基苯乙胺两个产物缩合修饰生成牛心果碱。上述构建的DY1工程菌可有效的将单糖或甘油转化为牛心果碱。
具体而言,本发明一方面涉及一种微生物,其特征在于所述微生物不含有或者敲除dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB基因,高表达或者导入aroGfbr、tyrAfb、KDC、HpaB、HpaC、DODC、CAR、sfp、aldH、CNS、4OMT、6OMT和CNMT基因。
进一步的,所述dkgB基因具有SEQ ID NO:1所示的核苷酸序列或者所述dkgB基因编码SEQ ID NO:2所示的氨基酸序列;
所述yeaE基因具有SEQ ID NO:3所示的核苷酸序列或者所述yeaE基因编码SEQ IDNO:4所示的氨基酸序列;
所述yqhC基因具有SEQ ID NO:5所示的核苷酸序列或者所述yqhC基因编码SEQ IDNO:6所示的氨基酸序列;
所述yqhD基因具有SEQ ID NO:7所示的核苷酸序列或者所述yqhD基因编码SEQ IDNO:8所示的氨基酸序列;
所述dkgA基因具有SEQ ID NO:9所示的核苷酸序列或者所述dkgA基因编码SEQ IDNO:10所示的氨基酸序列;
所述yahK基因具有SEQ ID NO:11所示的核苷酸序列或者所述yahK基因编码SEQID NO:12所示的氨基酸序列;
所述yjgB基因具有SEQ ID NO:13所示的核苷酸序列或者所述yjgB基因编码SEQID NO:14所示的氨基酸序列;
所述aroGfbr基因具有SEQ ID NO:15所示的核苷酸序列或者所述aroGfbr基因编码SEQ ID NO:16所示的氨基酸序列;
所述tyrAfb基因具有SEQ ID NO:17所示的核苷酸序列或者所述tyrAfb基因编码SEQ ID NO:18所示的氨基酸序列;
所述KDC基因具有SEQ ID NO:19所示的核苷酸序列或者所述KDC基因编码SEQ IDNO:20所示的氨基酸序列;
所述DODC基因具有SEQ ID NO:21所示的核苷酸序列或者所述DODC基因编码SEQID NO:22所示的氨基酸序列;
所述CNS基因具有SEQ ID NO:23所示的核苷酸序列或者所述CNS基因编码SEQ IDNO:24所示的氨基酸序列;
所述6OMT基因具有SEQ ID NO:25所示的核苷酸序列或者所述6OMT基因编码SEQID NO:26所示的氨基酸序列;
所述4OMT基因具有SEQ ID NO:27所示的核苷酸序列或者所述4OMT基因编码SEQID NO:28所示的氨基酸序列;
所述CNMT基因具有SEQ ID NO:29所示的核苷酸序列或者所述NMCH基因编码SEQID NO:30所示的氨基酸序列;
所述HpaB基因具有SEQ ID NO:31所示的核苷酸序列或者所述HpaB基因编码SEQID NO:32所示的氨基酸序列;
所述HpaC基因具有SEQ ID NO:33所示的核苷酸序列或者所述HpaC基因编码SEQID NO:34所示的氨基酸序列;
所述CAR基因具有SEQ ID NO:35所示的核苷酸序列或者所述CAR基因编码SEQ IDNO:36所示的氨基酸序列;
所述sfp基因具有SEQ ID NO:37所示的核苷酸序列或者所述sfp基因编码SEQ IDNO:38所示的氨基酸序列;
所述aldH基因具有SEQ ID NO:39所示的核苷酸序列或者所述aldH基因编码SEQID NO:40所示的氨基酸序列。
在本发明的一个优选实施方式中,微生物为大肠杆菌。
本发明另一方面还涉及上述微生物在生物合成牛心果碱中的应用。
作为微生物发酵发酵单糖或甘油合成牛心果碱的方法,所述的单糖为葡萄糖。
本发明的优点是在微生物中引入外源基因构建一条合成牛心果碱的代谢途径,最终实现以单糖或甘油为原料合成牛心果碱的目的,相比较与植物提取或化学合成可有效的降低成本。同时,微生物生长速度快,遗传操作技术成熟,且发酵过程不会对环境造成污染破坏。
附图说明
图1.牛心果碱生物合成路径图;
图2.大肠杆菌工程菌DY1和DY2发酵产物GC-MS检测结果。1号峰为内标4,4'-二甲氧基二苯甲酮,2号峰为产物牛心果碱。
图3,图2中发酵产物2号峰的离子碎片(上)与标准牛心果碱离子碎片(下)比较图。
具体实施方式
以下实施例用于进一步说明本发明,但不应理解为对本发明的限制。
实施例1构建产牛心果碱大肠杆菌工程菌
1.基因敲除菌株DY的构建
以pKD4质粒为模板,PCR扩增打靶片段,该片段包含(kanr序列和与待敲除目的基因两端互补的同源臂);将扩增的打靶片段电转到带有pKD46质粒的大肠杆菌中。利用pKD46质粒上带有的Red同源重组***,使待敲除目的基因与打靶片段进行重组,目的基因被抗性基因取代。随后将温敏质粒pCP20导入菌株,利用该质粒上的FLP翻转酶去除目的菌株染色体上的抗性基因。利用上述方法依次敲除大肠杆菌MG1655菌株中的dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB基因,获得大肠杆菌DY。所述dkgB基因具有SEQ ID NO:1所示的核苷酸序列;所述dkgB基因具有与SEQ ID NO:2所示的氨基酸序列;所述yeaE基因具有SEQ ID NO:3所示的核苷酸序列;所述yeaE基因具有与SEQ ID NO:4所示的氨基酸序列;所述yqhC基因具有SEQ ID NO:5所示的核苷酸序列;所述yqhC基因具有与SEQ ID NO:6所示的氨基酸序列;所述yqhD基因具有SEQ ID NO:7所示的核苷酸序列;所述yqhD基因具有与SEQ ID NO:8所示的氨基酸序列;所述dkgA基因具有SEQ ID NO:9所示的核苷酸序列;所述dkgA基因具有与SEQ ID NO:10所示的氨基酸序列;所述yahK基因具有SEQ ID NO:11所示的核苷酸序列;所述yahK基因具有与SEQ ID NO:12所示的氨基酸序列;所述yjgB基因具有SEQ ID NO:13所示的核苷酸序列;所述yjgB基因具有与SEQ ID NO:14所示的氨基酸序列;敲除基因用的引物为:
DkgB-F:atggctatccctgcatttggtttaggtactttccgtctgagtgtaggctggagctgcttc
DkgB-R:ttaatcccattcaggagccagaccttccgggctaaccaggcggcatatgaatatcctcctta
yeaE-F:atgcaacaaaaaatgattcaatttagtggcgatgtctcactggtgtaggctggagctgcttc
yeaE-R:tcacaccatatccagcgcagtttttccttttggtgccggatatcatatgaatatcctcctta
yqhC-F:atgctacaaaattgcgcacaatcaaattgccgcattattcctaagtgtaggctggagctgcttc
yqhC-R:ttaattcccctgcatcgcccgcattcttgccgcatcttcccccatatgaatatcctcctta
yqhD-F:atgaacaactttaatctgcacaccccaacccgcattctgttgtgtaggctggagctgcttc
yqhD-R:ttagcgggcggcttcgtatatacggcggctgacatccaacgcatatgaatatcctcctta
dkgA-F:atggctaatccaaccgttattaagctacaggatggcaatgtcgtgtaggctggagctgcttc
dkgA-R:ttagccgccgaactggtcaggatcgggaccgagacgcttgccatatgaatatcctcctta
yahK-F:atgaagatcaaagctgttggtgcatattccgctaaacaaccgtgtaggctggagctgcttc
yahK-R:tcagtctgttagtgtgcgattatcgataacaaaacgatattcatatgaatatcctcctta
yjgB-F:atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgtgtaggctggagctgcttc
yjgB-R:tcaaaaatcggctttcaacaccacgcggtaacgcgccttaccgtcatatgaatatcctcctta
2.载体pDY001的构建
解除反馈抑制的aroGfbr和tyrAfbr基因委托金唯智生物科技有限公司合成,以合成的基因为模板利用引物aroGfbr-XbaI(gtatctagaaagaggagatataatgaattatcagaacgacgatttacgc)和aroGfbr-SpeI-BamHI(tatggatccactagtttacccgcgacgcgctttta)PCR扩增aroGfbr基因,随后将扩增的片段以XbaI和BamHI***到pET28a(+)中得到载体pDY1。以合成的基因为模板,利用引物tyrAfbrr-XbaI(gtatctagaaagaggagatataatggttgctgaattgaccgcat)和tyrAfbr-SpeI-SalI(atatgtcgacactagtttactggcgattgtcattcgcc)PCR扩增来tyrAfbr基因,随后将扩增的片段以XbaI和SalI双酶切,***到以SpeI和SalI双酶切的载体pDY1上,中得到载体pDY2。利用引物KDC-XbaI(atctctagatttaagaaggagatataatggcacctgttacaattgaaaagttc)和KDC-NheI-BamHI(tctggatccgctagcctattttttatttcttttaagtgccgctg)PCR扩增来自酿酒酵母菌YPH499的KDC基因,随后将扩增的片段以XbaI和BamHI双酶切,将其***以XbaI和BamHI双酶切的载体pBBR1MCS1上,得到载体pDY3。利用XbaI和SalI双酶切pDY2得到aroGfbr和tyrAfbr表达框,将其***以NheI和XhoI双酶切的载体pDY3,得到载体pDY001。所述aroGfbr基因具有SEQ ID NO:15所示的核苷酸序列;所述aroGfbr基因具有与SEQ ID NO:16所示的氨基酸序列;所述tyrAfb基因具有SEQ ID NO:17所示的核苷酸序列编码;所述tyrAfb基因具有SEQ ID NO:18所示的氨基酸序列;所述KDC基因具有SEQ ID NO:19所示的核苷酸序列编码;所述KDC基因具有SEQ ID NO:20所示的氨基酸序列。
3.载体pDY002的构建
DODC、CNS、6OMT、4OMT和CNMT基因委托金唯智生物科技有限公司合成。以合成的基因为模板,利用引物DODC-XbaI(tagtctagatttaagaaggagatataatgacccccgaacagttccg)/DODC-SpeI-BamHI(acaggatccactagtttagctgccgcctttaatcacgt)、CNS-XbaI(tactctagatttaagaaggagatataatgcgtaaagtgatcaaatatgatatgg)/CNS-SpeI-BamHI(tcaggatccactagtttagctgcccagtaaacgaggaa)、6OMT-XbaI(ttgtctagatttaagaaggagatataatggagaccgttagcaaaattgacc)/6OMT-SpeI-BamHI(gcaggatccactagtttagtacggatatgcttcaatcacgct)、4OMT-XbaI(tactctagatttaagaaggagatataatggccttccatggcaaggac)/4OMT-SpeI-BamHI(tcaggatccactagtttaaggaaacacctcaatcacgctct)、CNMT-XbaI(atctctagatttaagaaggagatataatgcagttaaaagaaaaagaggagctg)/CNMT-SpeI-BamHI(agaggatccactagtttattttttcttaaacagcagatgggttaac),分别PCR扩增DODC、CNS、6OMT、4OMT和CNMT基因,随后将扩增的片段以XbaI和BamHI***到pET28a(+)中,依次得到载体pDY4、pDY5、pDY6、pDY7和pDY8。利用XbaI和XhoI双酶切pDY5得到CNS表达框,将其***以SpeI和XhoI双酶切的载体pDY4上,得到载体pDY9。利用XbaI和XhoI双酶切pDY6得到6OMT表达框,将其***以SpeI和XhoI双酶切的载体pDY9上,得到载体pDY10。利用XbaI和XhoI双酶切pDY7得到4OMT表达框,将其***以SpeI和XhoI双酶切的载体pDY10上,得到载体pDY11。利用XbaI和XhoI双酶切pDY8得到CNMT表达框,将其***以SpeI和XhoI双酶切的载体pDY11上,得到载体pDY12。以大肠杆菌MG1655的基因组为模板,利用引物HpaBC-XbaI(tagtctagatttaagaaggagatataatgaaaccagaagatttccgcg)和HpaBC-XhoI(aacgctcgagttaaatcgcagcttccatttcca),PCR扩增HpaBC基因(包含HpaB和HpaC两个基因),随后将扩增的片段以XbaI和XhoI双酶切,将其***以SpeI和XhoI双酶切的载体pDY12上,得到载体pDY002。所述DODC基因具有SEQ ID NO:21所示的核苷酸序列;所述DODC基因具有与SEQ ID NO:22所示的氨基酸序列;所述CNS基因具有SEQ ID NO:23所示的核苷酸序列;所述CNS基因具有与SEQ ID NO:24所示的氨基酸序列;所述6OMT基因具有SEQ IDNO:25所示的核苷酸序列;所述6OMT基因具有与SEQ ID NO:26所示的氨基酸序列;所述4OMT基因具有SEQ ID NO:27所示的核苷酸序列;所述4OMT基因具有与SEQ ID NO:28所示的氨基酸序列;所述CNMT基因具有SEQ ID NO:29所示的核苷酸序列;所述CNMT基因具有与SEQ IDNO:30所示的氨基酸序列;所述HpaB基因具有SEQ ID NO:31所示的核苷酸序列;所述HpaB基因具有与SEQ ID NO:32所示的氨基酸序列;所述HpaC基因具有SEQ ID NO:33所示的核苷酸序列;所述HpaC基因具有与SEQ ID NO:34所示的氨基酸序列;
4.载体pDY003的构建
CAR基因委托金唯智生物科技有限公司合成。以合成的基因为模板,利用引物CAR-XbaI(atctctagatttaagaaggagatataatgagtccgatcacacgcgag)/CAR-SpeI-BamHI(agaggatccactagtttacagcaggcctaacaggcg)PCR扩增CAR基因,随后将扩增的片段以XbaI和BamHI双酶切,***到以XbaI和BamHI双酶切的pET28a(+)中,得到载体pDY13。以芽孢杆菌Bacillussubtilis subsp.subtilisstr.168的基因组为模板,利用引物sfp-XbaI(gtctctagatttaagaaggagatataatgaagatttacggaatttatatggaccg)/sfp-SpeI-BamHI(tgaggatccactagtttataaaagctcttcgtacgagaccattgt)PCR扩增sfp基因,随后将扩增的片段以XbaI和BamHI双酶切,***到以XbaI和BamHI双酶切的pET28a(+)中,得到载体pDY14。以大肠杆菌MG1655的基因组为模板,利用引物aldH-XbaI(atctctagatttaagaaggagatataatgaattttcatcatctggcttactg)/aldH-NheI-BamHI(tcaggatccgctagctcaggcctccaggctatcc)PCR扩增aldH基因,随后将扩增的片段以XbaI和BamHI双酶切,***到以XbaI和BamHI双酶切的pET28a(+)中,得到载体pDY15。利用XbaI和XhoI双酶切pDY14得到sfp表达框,将其***以SpeI和XhoI双酶切的载体pDY13上,得到载体pDY16。利用XbaI和XhoI双酶切pDY15得到aldH表达框,将其***以SpeI和XhoI双酶切的载体pDY16上,得到载体pDY17。利用XbaI和XhoI双酶切pDY17得到CAR、sfp和aldH表达框,将其***以XbaI和XhoI双酶切的载体pET28b-pSC101上,得到载体pDY003。所述CAR基因具有SEQ ID NO:35所示的核苷酸序列;所述CAR基因具有与SEQ IDNO:36所示的氨基酸序列;所述sfp基因具有SEQ ID NO:37所示的核苷酸序列;所述sfp基因具有与SEQ ID NO:38所示的氨基酸序列;所述aldH基因具有SEQ ID NO:39所示的核苷酸序列;所述aldH基因具有与SEQ ID NO:40所示的氨基酸序列;
5.将重组载体pDY001和pDY002同时导入大肠杆菌DY中,得到DY1工程菌。该工程菌可实现aroGfbr、tyrAfbr、KDC、HpaBC、DODC、CNS、6OMT、4OMT和CNMT基因的高表达,可有效的将单糖或甘油转化为牛心果碱。
5.将重组载体pDY001、pDY002和pDY003同时导入大肠杆菌DY中,得到DY2工程菌。该工程菌可实现aroGfbr、tyrAfbr、KDC、HpaBC、DODC、CNS、6OMT、4OMT、CNMT、CAR、sfp和aldH基因的高表达。相较于DY1工程菌,进一步实现CAR、sfp和aldH基因高效表达的DY2工程菌,可提升的将单糖或甘油转化为牛心果碱的生物合成效率。
实施例2大肠杆菌工程菌DY1生产牛心果碱的发酵实验
1、将37度LB中过夜培养的大肠杆菌工程菌DY1,接种到50ml M9发酵培养基中(包含2%葡萄糖),进行30度摇瓶发酵,待菌体OD600为0.8左右,加入0.1mM IPTG进行目的基因的诱导表达。
2、发酵培养24小时后收集菌体,超声波破细胞,利用氯仿萃取产物。
3、利用旋转蒸发仪将溶剂氯仿旋干,再用一定量的氯仿溶解产物。
4、GC-MS(气相色谱-质谱联用仪)检测样品。
5、实验结果:大肠杆菌工程菌利用葡萄糖合成牛心果碱的GC-MS检测结果如图2A所示,牛心果碱GC-MS鉴定如图3所示,表明菌株DY1可有效利用葡萄糖或甘油合成牛心果碱。
实施例3大肠杆菌工程菌DY2生产牛心果碱的发酵实验
1、将37度LB中过夜培养的大肠杆菌工程菌DY2,接种到50ml M9发酵培养基中(包含2%葡萄糖),进行30度摇瓶发酵,待菌体OD600为0.8左右,加入0.1mM IPTG进行目的基因的诱导表达。
2、发酵培养24小时后收集菌体,超声波破细胞,利用氯仿萃取产物。
3、利用旋转蒸发仪将溶剂氯仿旋干,再用一定量的氯仿溶解产物。
4、GC-MS(气相色谱-质谱联用仪)检测样品。
5、实验结果:大肠杆菌工程菌DY2利用葡萄糖合成牛心果碱的GC-MS检测结果如图2B,表明菌株DY2可有效利用葡萄糖或甘油合成牛心果碱。
以上所述仅是本发明的优选实施方案,应当指出,对于本技术领域的技术人员,在不脱离本分明原理的前提下,还可以做出适当的改进,这些改进也应视为本发明的保护范围。
序列表
<110> 赣南师范大学
<120> 一种生物合成牛心果碱的微生物及其方法
<160> 78
<170> SIPOSequenceListing 1.0
<210> 1
<211> 804
<212> DNA
<213> Unkown
<400> 1
atggctatcc ctgcatttgg tttaggtact ttccgtctga aagacgacgt tgttatttca 60
tctgtgataa cggcgcttga acttggttat cgcgcaattg ataccgcaca aatctatgat 120
aacgaagccg cagtaggtca ggcgattgca gaaagtggcg tgccacgtca tgaactctac 180
atcaccacta aaatctggat tgaaaatctc agcaaagaca aattgatccc aagtctgaaa 240
gagagcctgc aaaaattgcg taccgattat gttgatctga cgctaatcca ctggccgtca 300
ccaaacgatg aagtctctgt tgaagagttt atgcaggcgc tgctggaagc caaaaaacaa 360
gggctgacgc gtgagatcgg tatttccaac ttcacgatcc cgttgatgga aaaagcgatt 420
gctgctgttg gtgctgaaaa catcgctact aaccagattg aactctctcc ttatctgcaa 480
aaccgtaaag tggttgcctg ggctaaacag cacggcatcc atattacttc ctatatgacg 540
ctggcgtatg gtaaggccct gaaagatgag gttattgctc gtatcgcagc taaacacaat 600
gcgactccgg cacaagtgat tctggcgtgg gctatggggg aaggttactc agtaattcct 660
tcttctacta aacgtaaaaa cctggaaagt aatcttaagg cacaaaattt acagcttgat 720
gccgaagata aaaaagcgat cgccgcactg gattgcaacg accgcctggt tagcccggaa 780
ggtctggctc ctgaatggga ttaa 804
<210> 2
<211> 267
<212> PRT
<213> Unkown
<400> 2
Met Ala Ile Pro Ala Phe Gly Leu Gly Thr Phe Arg Leu Lys Asp Asp
1 5 10 15
Val Val Ile Ser Ser Val Ile Thr Ala Leu Glu Leu Gly Tyr Arg Ala
20 25 30
Ile Asp Thr Ala Gln Ile Tyr Asp Asn Glu Ala Ala Val Gly Gln Ala
35 40 45
Ile Ala Glu Ser Gly Val Pro Arg His Glu Leu Tyr Ile Thr Thr Lys
50 55 60
Ile Trp Ile Glu Asn Leu Ser Lys Asp Lys Leu Ile Pro Ser Leu Lys
65 70 75 80
Glu Ser Leu Gln Lys Leu Arg Thr Asp Tyr Val Asp Leu Thr Leu Ile
85 90 95
His Trp Pro Ser Pro Asn Asp Glu Val Ser Val Glu Glu Phe Met Gln
100 105 110
Ala Leu Leu Glu Ala Lys Lys Gln Gly Leu Thr Arg Glu Ile Gly Ile
115 120 125
Ser Asn Phe Thr Ile Pro Leu Met Glu Lys Ala Ile Ala Ala Val Gly
130 135 140
Ala Glu Asn Ile Ala Thr Asn Gln Ile Glu Leu Ser Pro Tyr Leu Gln
145 150 155 160
Asn Arg Lys Val Val Ala Trp Ala Lys Gln His Gly Ile His Ile Thr
165 170 175
Ser Tyr Met Thr Leu Ala Tyr Gly Lys Ala Leu Lys Asp Glu Val Ile
180 185 190
Ala Arg Ile Ala Ala Lys His Asn Ala Thr Pro Ala Gln Val Ile Leu
195 200 205
Ala Trp Ala Met Gly Glu Gly Tyr Ser Val Ile Pro Ser Ser Thr Lys
210 215 220
Arg Lys Asn Leu Glu Ser Asn Leu Lys Ala Gln Asn Leu Gln Leu Asp
225 230 235 240
Ala Glu Asp Lys Lys Ala Ile Ala Ala Leu Asp Cys Asn Asp Arg Leu
245 250 255
Val Ser Pro Glu Gly Leu Ala Pro Glu Trp Asp
260 265
<210> 3
<211> 855
<212> DNA
<213> Unkown
<400> 3
atgcaacaaa aaatgattca atttagtggc gatgtctcac tgccagccgt agggcaggga 60
acatggtata tgggcgaaga tgccagtcag cgcaaaacag aagttgctgc actacgcgcg 120
ggcattgaac tcggtttaac cctcattgat accgccgaaa tgtatgccga tggcggtgcc 180
gaaaaggtgg ttggggaagc attaaccggt ctgcgagaga aggtctttct cgtctctaaa 240
gtctatccgt ggaatgctgg cgggcaaaaa gcgataaatg catgcgaagc cagtttacgc 300
cgtctcaata ctgattatct cgatctttac ttattacact ggtctggcag tttcgctttt 360
gaagagactg tcgcagcgat ggaaaaattg atcgcccagg gaaaaatccg ccgctggggc 420
gtttctaacc ttgattatgc tgatatgcag gaactctggc agctgccggg gggaaatcag 480
tgtgccacta atcaggtgct ttaccatctc ggttcacgag gaattgagta cgatctactc 540
ccctggtgcc agcaacagca gatgccggtg atggcttaca gtccgttagc ccaggccggg 600
cggttgcgca atggactgtt aaaaaacgcg gtagtcaacg aaattgcaca tgctcacaat 660
atcagcgcgg cacaagtatt gttggcgtgg gtgatcagtc atcagggtgt gatggcgatt 720
ccaaaagcgg ccacgattgc ccatgtccaa caaaatgcgg ctgtgcttga ggtcgaactt 780
tcttcagcgg aattagctat gctggataag gcatatccgg caccaaaagg aaaaactgcg 840
ctggatatgg tgtga 855
<210> 4
<211> 284
<212> PRT
<213> Unkown
<400> 4
Met Gln Gln Lys Met Ile Gln Phe Ser Gly Asp Val Ser Leu Pro Ala
1 5 10 15
Val Gly Gln Gly Thr Trp Tyr Met Gly Glu Asp Ala Ser Gln Arg Lys
20 25 30
Thr Glu Val Ala Ala Leu Arg Ala Gly Ile Glu Leu Gly Leu Thr Leu
35 40 45
Ile Asp Thr Ala Glu Met Tyr Ala Asp Gly Gly Ala Glu Lys Val Val
50 55 60
Gly Glu Ala Leu Thr Gly Leu Arg Glu Lys Val Phe Leu Val Ser Lys
65 70 75 80
Val Tyr Pro Trp Asn Ala Gly Gly Gln Lys Ala Ile Asn Ala Cys Glu
85 90 95
Ala Ser Leu Arg Arg Leu Asn Thr Asp Tyr Leu Asp Leu Tyr Leu Leu
100 105 110
His Trp Ser Gly Ser Phe Ala Phe Glu Glu Thr Val Ala Ala Met Glu
115 120 125
Lys Leu Ile Ala Gln Gly Lys Ile Arg Arg Trp Gly Val Ser Asn Leu
130 135 140
Asp Tyr Ala Asp Met Gln Glu Leu Trp Gln Leu Pro Gly Gly Asn Gln
145 150 155 160
Cys Ala Thr Asn Gln Val Leu Tyr His Leu Gly Ser Arg Gly Ile Glu
165 170 175
Tyr Asp Leu Leu Pro Trp Cys Gln Gln Gln Gln Met Pro Val Met Ala
180 185 190
Tyr Ser Pro Leu Ala Gln Ala Gly Arg Leu Arg Asn Gly Leu Leu Lys
195 200 205
Asn Ala Val Val Asn Glu Ile Ala His Ala His Asn Ile Ser Ala Ala
210 215 220
Gln Val Leu Leu Ala Trp Val Ile Ser His Gln Gly Val Met Ala Ile
225 230 235 240
Pro Lys Ala Ala Thr Ile Ala His Val Gln Gln Asn Ala Ala Val Leu
245 250 255
Glu Val Glu Leu Ser Ser Ala Glu Leu Ala Met Leu Asp Lys Ala Tyr
260 265 270
Pro Ala Pro Lys Gly Lys Thr Ala Leu Asp Met Val
275 280
<210> 5
<211> 957
<212> DNA
<213> Unkown
<400> 5
atgctacaaa attgcgcaca atcaaattgc cgcattattc ctaagaaatt acgcgatatg 60
aaacgtgaag agatttgccg cttgctggcg gataaagtta ataaactgaa aaataaagaa 120
aatagtttgt caggactgtt gcccgatgtg cgtttgttgt atggcgagac gcctttcgca 180
cgtacaccgg tgatgtacga gcctggcatc ataattctct tttccgggca taaaatcggt 240
tatatcaatg aacgcgtgtt tcgttatgat gccaatgaat acctgctgct gacggtgccg 300
ttgccgtttg agtgcgaaac ctatgccacg tcagaggtgc cgctggcagg gttgcgtctc 360
aatgtcgata ttttgcagtt acaggaactg ttgatggaca ttggcgaaga tgagcatttc 420
cagccgtcga tggcagccag cgggattaac tccgccacgt tatcagaaga gattttatgc 480
gcggcggagc ggttactcga cgtgatggag cgaccactgg atgcgcgtat tctcggcaaa 540
cagatcatcc gcgaaattct gtactacgtg ctgaccggac cttgcggcgg cgcgttactg 600
gcgctggtca gtcgccagac tcacttcagt ctgattagcc gcgtgctgaa acggattgag 660
aataaataca ccgaaaacct gagcgtcgag caactggcgg cagaagccaa catgagcgta 720
tcggcgttcc accataattt taagtctgtc accagtacct cgccgttgca gtatttgaag 780
aattaccgtc tgcataaggc gcggatgatg atcatccatg acggcatgaa ggccagcgca 840
gcagcgatgc gcgtcggcta tgaaagcgca tcgcaattta gccgtgagtt taaacgttac 900
ttcggtgtga cgccggggga agatgcggca agaatgcggg cgatgcaggg gaattaa 957
<210> 6
<211> 318
<212> PRT
<213> Unkown
<400> 6
Met Leu Gln Asn Cys Ala Gln Ser Asn Cys Arg Ile Ile Pro Lys Lys
1 5 10 15
Leu Arg Asp Met Lys Arg Glu Glu Ile Cys Arg Leu Leu Ala Asp Lys
20 25 30
Val Asn Lys Leu Lys Asn Lys Glu Asn Ser Leu Ser Gly Leu Leu Pro
35 40 45
Asp Val Arg Leu Leu Tyr Gly Glu Thr Pro Phe Ala Arg Thr Pro Val
50 55 60
Met Tyr Glu Pro Gly Ile Ile Ile Leu Phe Ser Gly His Lys Ile Gly
65 70 75 80
Tyr Ile Asn Glu Arg Val Phe Arg Tyr Asp Ala Asn Glu Tyr Leu Leu
85 90 95
Leu Thr Val Pro Leu Pro Phe Glu Cys Glu Thr Tyr Ala Thr Ser Glu
100 105 110
Val Pro Leu Ala Gly Leu Arg Leu Asn Val Asp Ile Leu Gln Leu Gln
115 120 125
Glu Leu Leu Met Asp Ile Gly Glu Asp Glu His Phe Gln Pro Ser Met
130 135 140
Ala Ala Ser Gly Ile Asn Ser Ala Thr Leu Ser Glu Glu Ile Leu Cys
145 150 155 160
Ala Ala Glu Arg Leu Leu Asp Val Met Glu Arg Pro Leu Asp Ala Arg
165 170 175
Ile Leu Gly Lys Gln Ile Ile Arg Glu Ile Leu Tyr Tyr Val Leu Thr
180 185 190
Gly Pro Cys Gly Gly Ala Leu Leu Ala Leu Val Ser Arg Gln Thr His
195 200 205
Phe Ser Leu Ile Ser Arg Val Leu Lys Arg Ile Glu Asn Lys Tyr Thr
210 215 220
Glu Asn Leu Ser Val Glu Gln Leu Ala Ala Glu Ala Asn Met Ser Val
225 230 235 240
Ser Ala Phe His His Asn Phe Lys Ser Val Thr Ser Thr Ser Pro Leu
245 250 255
Gln Tyr Leu Lys Asn Tyr Arg Leu His Lys Ala Arg Met Met Ile Ile
260 265 270
His Asp Gly Met Lys Ala Ser Ala Ala Ala Met Arg Val Gly Tyr Glu
275 280 285
Ser Ala Ser Gln Phe Ser Arg Glu Phe Lys Arg Tyr Phe Gly Val Thr
290 295 300
Pro Gly Glu Asp Ala Ala Arg Met Arg Ala Met Gln Gly Asn
305 310 315
<210> 7
<211> 1164
<212> DNA
<213> Unkown
<400> 7
atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct 60
ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg cggcggcagc 120
gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg 180
gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg 240
gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc 300
accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg 360
caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca 420
gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag 480
caggcgttcc attctgccca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc 540
tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg 600
gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt 660
ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg 720
cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cgctggcgta 780
ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat 840
cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgataccaag 900
cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgatgat 960
gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg 1020
acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg 1080
gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc 1140
cgtatatacg aagccgcccg ctaa 1164
<210> 8
<211> 387
<212> PRT
<213> Unkown
<400> 8
Met Asn Asn Phe Asn Leu His Thr Pro Thr Arg Ile Leu Phe Gly Lys
1 5 10 15
Gly Ala Ile Ala Gly Leu Arg Glu Gln Ile Pro His Asp Ala Arg Val
20 25 30
Leu Ile Thr Tyr Gly Gly Gly Ser Val Lys Lys Thr Gly Val Leu Asp
35 40 45
Gln Val Leu Asp Ala Leu Lys Gly Met Asp Val Leu Glu Phe Gly Gly
50 55 60
Ile Glu Pro Asn Pro Ala Tyr Glu Thr Leu Met Asn Ala Val Lys Leu
65 70 75 80
Val Arg Glu Gln Lys Val Thr Phe Leu Leu Ala Val Gly Gly Gly Ser
85 90 95
Val Leu Asp Gly Thr Lys Phe Ile Ala Ala Ala Ala Asn Tyr Pro Glu
100 105 110
Asn Ile Asp Pro Trp His Ile Leu Gln Thr Gly Gly Lys Glu Ile Lys
115 120 125
Ser Ala Ile Pro Met Gly Cys Val Leu Thr Leu Pro Ala Thr Gly Ser
130 135 140
Glu Ser Asn Ala Gly Ala Val Ile Ser Arg Lys Thr Thr Gly Asp Lys
145 150 155 160
Gln Ala Phe His Ser Ala His Val Gln Pro Val Phe Ala Val Leu Asp
165 170 175
Pro Val Tyr Thr Tyr Thr Leu Pro Pro Arg Gln Val Ala Asn Gly Val
180 185 190
Val Asp Ala Phe Val His Thr Val Glu Gln Tyr Val Thr Lys Pro Val
195 200 205
Asp Ala Lys Ile Gln Asp Arg Phe Ala Glu Gly Ile Leu Leu Thr Leu
210 215 220
Ile Glu Asp Gly Pro Lys Ala Leu Lys Glu Pro Glu Asn Tyr Asp Val
225 230 235 240
Arg Ala Asn Val Met Trp Ala Ala Thr Gln Ala Leu Asn Gly Leu Ile
245 250 255
Gly Ala Gly Val Pro Gln Asp Trp Ala Thr His Met Leu Gly His Glu
260 265 270
Leu Thr Ala Met His Gly Leu Asp His Ala Gln Thr Leu Ala Ile Val
275 280 285
Leu Pro Ala Leu Trp Asn Glu Lys Arg Asp Thr Lys Arg Ala Lys Leu
290 295 300
Leu Gln Tyr Ala Glu Arg Val Trp Asn Ile Thr Glu Gly Ser Asp Asp
305 310 315 320
Glu Arg Ile Asp Ala Ala Ile Ala Ala Thr Arg Asn Phe Phe Glu Gln
325 330 335
Leu Gly Val Pro Thr His Leu Ser Asp Tyr Gly Leu Asp Gly Ser Ser
340 345 350
Ile Pro Ala Leu Leu Lys Lys Leu Glu Glu His Gly Met Thr Gln Leu
355 360 365
Gly Glu Asn His Asp Ile Thr Leu Asp Val Ser Arg Arg Ile Tyr Glu
370 375 380
Ala Ala Arg
385
<210> 9
<211> 828
<212> DNA
<213> Unkown
<400> 9
atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg 60
ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg 120
ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc 180
ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac 240
gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat 300
atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg 360
aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc 420
cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag 480
atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa 540
atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa 600
gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg 660
catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa 720
aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc 780
gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaa 828
<210> 10
<211> 275
<212> PRT
<213> Unkown
<400> 10
Met Ala Asn Pro Thr Val Ile Lys Leu Gln Asp Gly Asn Val Met Pro
1 5 10 15
Gln Leu Gly Leu Gly Val Trp Gln Ala Ser Asn Glu Glu Val Ile Thr
20 25 30
Ala Ile Gln Lys Ala Leu Glu Val Gly Tyr Arg Ser Ile Asp Thr Ala
35 40 45
Ala Ala Tyr Lys Asn Glu Glu Gly Val Gly Lys Ala Leu Lys Asn Ala
50 55 60
Ser Val Asn Arg Glu Glu Leu Phe Ile Thr Thr Lys Leu Trp Asn Asp
65 70 75 80
Asp His Lys Arg Pro Arg Glu Ala Leu Leu Asp Ser Leu Lys Lys Leu
85 90 95
Gln Leu Asp Tyr Ile Asp Leu Tyr Leu Met His Trp Pro Val Pro Ala
100 105 110
Ile Asp His Tyr Val Glu Ala Trp Lys Gly Met Ile Glu Leu Gln Lys
115 120 125
Glu Gly Leu Ile Lys Ser Ile Gly Val Cys Asn Phe Gln Ile His His
130 135 140
Leu Gln Arg Leu Ile Asp Glu Thr Gly Val Thr Pro Val Ile Asn Gln
145 150 155 160
Ile Glu Leu His Pro Leu Met Gln Gln Arg Gln Leu His Ala Trp Asn
165 170 175
Ala Thr His Lys Ile Gln Thr Glu Ser Trp Ser Pro Leu Ala Gln Gly
180 185 190
Gly Lys Gly Val Phe Asp Gln Lys Val Ile Arg Asp Leu Ala Asp Lys
195 200 205
Tyr Gly Lys Thr Pro Ala Gln Ile Val Ile Arg Trp His Leu Asp Ser
210 215 220
Gly Leu Val Val Ile Pro Lys Ser Val Thr Pro Ser Arg Ile Ala Glu
225 230 235 240
Asn Phe Asp Val Trp Asp Phe Arg Leu Asp Lys Asp Glu Leu Gly Glu
245 250 255
Ile Ala Lys Leu Asp Gln Gly Lys Arg Leu Gly Pro Asp Pro Asp Gln
260 265 270
Phe Gly Gly
275
<210> 11
<211> 1050
<212> DNA
<213> Unkown
<400> 11
atgaagatca aagctgttgg tgcatattcc gctaaacaac cacttgaacc gatggatatc 60
acccggcgtg aaccgggacc gaatgatgtc aaaatcgaaa tcgcttactg tggcgtttgc 120
cattccgatc tccaccaggt ccgttccgag tgggcgggga cggtttaccc ctgcgtgccg 180
ggtcatgaaa ttgtggggcg tgtggtagcc gttggtgatc aggtagaaaa atatgcgccg 240
ggcgatctgg tcggtgtcgg ctgcattgtc gacagttgta aacattgcga agagtgtgaa 300
gacgggttgg aaaactactg tgatcacatg accggcacct ataactcgcc gacgccggac 360
gaaccgggcc atactctggg cggctactca caacagatcg tcgttcatga gcgatatgtt 420
ctgcgtattc gtcacccgca agagcagctg gcggcggtgg ctcctttgtt gtgtgcaggg 480
atcaccacgt attcgccgct acgtcactgg caggccgggc cgggtaaaaa agtgggcgtg 540
gtcggcatcg gcggtctggg acatatgggg attaagctgg cccacgcgat gggggcacat 600
gtggtggcat ttaccacttc tgaggcaaaa cgcgaagcgg caaaagccct gggggccgat 660
gaagttgtta actcacgcaa tgccgatgag atggcggctc atctgaagag tttcgatttc 720
attttgaata cagtagctgc gccacataat ctcgacgatt ttaccacctt gctgaagcgt 780
gatggcacca tgacgctggt tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc 840
aacctgatca tgaaacgccg tgcgatagcc ggttctatga ttggcggcat tccagaaact 900
caggagatgc tcgatttttg cgccgaacat ggcatcgtgg ctgatataga gatgattcgg 960
gccgatcaaa ttaatgaagc ctatgagcga atgctgcgcg gtgatgtgaa atatcgtttt 1020
gttatcgata atcgcacact aacagactga 1050
<210> 12
<211> 349
<212> PRT
<213> Unkown
<400> 12
Met Lys Ile Lys Ala Val Gly Ala Tyr Ser Ala Lys Gln Pro Leu Glu
1 5 10 15
Pro Met Asp Ile Thr Arg Arg Glu Pro Gly Pro Asn Asp Val Lys Ile
20 25 30
Glu Ile Ala Tyr Cys Gly Val Cys His Ser Asp Leu His Gln Val Arg
35 40 45
Ser Glu Trp Ala Gly Thr Val Tyr Pro Cys Val Pro Gly His Glu Ile
50 55 60
Val Gly Arg Val Val Ala Val Gly Asp Gln Val Glu Lys Tyr Ala Pro
65 70 75 80
Gly Asp Leu Val Gly Val Gly Cys Ile Val Asp Ser Cys Lys His Cys
85 90 95
Glu Glu Cys Glu Asp Gly Leu Glu Asn Tyr Cys Asp His Met Thr Gly
100 105 110
Thr Tyr Asn Ser Pro Thr Pro Asp Glu Pro Gly His Thr Leu Gly Gly
115 120 125
Tyr Ser Gln Gln Ile Val Val His Glu Arg Tyr Val Leu Arg Ile Arg
130 135 140
His Pro Gln Glu Gln Leu Ala Ala Val Ala Pro Leu Leu Cys Ala Gly
145 150 155 160
Ile Thr Thr Tyr Ser Pro Leu Arg His Trp Gln Ala Gly Pro Gly Lys
165 170 175
Lys Val Gly Val Val Gly Ile Gly Gly Leu Gly His Met Gly Ile Lys
180 185 190
Leu Ala His Ala Met Gly Ala His Val Val Ala Phe Thr Thr Ser Glu
195 200 205
Ala Lys Arg Glu Ala Ala Lys Ala Leu Gly Ala Asp Glu Val Val Asn
210 215 220
Ser Arg Asn Ala Asp Glu Met Ala Ala His Leu Lys Ser Phe Asp Phe
225 230 235 240
Ile Leu Asn Thr Val Ala Ala Pro His Asn Leu Asp Asp Phe Thr Thr
245 250 255
Leu Leu Lys Arg Asp Gly Thr Met Thr Leu Val Gly Ala Pro Ala Thr
260 265 270
Pro His Lys Ser Pro Glu Val Phe Asn Leu Ile Met Lys Arg Arg Ala
275 280 285
Ile Ala Gly Ser Met Ile Gly Gly Ile Pro Glu Thr Gln Glu Met Leu
290 295 300
Asp Phe Cys Ala Glu His Gly Ile Val Ala Asp Ile Glu Met Ile Arg
305 310 315 320
Ala Asp Gln Ile Asn Glu Ala Tyr Glu Arg Met Leu Arg Gly Asp Val
325 330 335
Lys Tyr Arg Phe Val Ile Asp Asn Arg Thr Leu Thr Asp
340 345
<210> 13
<211> 1020
<212> DNA
<213> Unkown
<400> 13
atgtcgatga taaaaagcta tgccgcaaaa gaagcgggcg gcgaactgga agtttatgag 60
tacgatcccg gtgagctgag gccacaagat gttgaagtgc aggtggatta ctgcgggatc 120
tgccattccg atctgtcgat gatcgataac gaatggggat tttcacaata tccgctggtt 180
gccgggcatg aggtgattgg gcgcgtggtg gcactcggga gcgccgcgca ggataaaggt 240
ttgcaggtcg gtcagcgtgt cgggattggc tggacggcgc gtagctgtgg tcactgcgac 300
gcctgtatta gcggtaatca gatcaactgc gagcaaggtg cggtgccgac gattatgaat 360
cgcggtggct ttgccgagaa gttgcgtgcg gactggcaat gggtgattcc actgccagaa 420
aatattgata tcgagtccgc cgggccgctg ttgtgcggcg gtatcacggt ctttaaacca 480
ctgttgatgc accatatcac tgctaccagc cgcgttgggg taattggtat tggcgggctg 540
gggcatatcg ctataaaact tctgcacgca atgggatgcg aggtgacagc ctttagttct 600
aatccggcga aagagcagga agtgctggcg atgggtgccg ataaagtggt gaatagccgc 660
gatccgcagg cactgaaagc actggcgggg cagtttgatc tcattatcaa caccgtcaac 720
gtcagcctcg actggcagcc ctattttgag gcgctgacct atggcggtaa tttccatacg 780
gtcggtgcgg ttctcacgcc gctgtctgtt ccggccttta cgttaattgc gggcgatcgc 840
agcgtctctg gttctgctac cggcacgcct tatgagctgc gtaagctgat gcgttttgcc 900
gcccgcagca aggttgcgcc gaccaccgaa ctgttcccga tgtcgaaaat taacgacgcc 960
atccagcatg tgcgcgacgg taaggcgcgt taccgcgtgg tgttgaaagc cgatttttga 1020
<210> 14
<211> 339
<212> PRT
<213> Unkown
<400> 14
Met Ser Met Ile Lys Ser Tyr Ala Ala Lys Glu Ala Gly Gly Glu Leu
1 5 10 15
Glu Val Tyr Glu Tyr Asp Pro Gly Glu Leu Arg Pro Gln Asp Val Glu
20 25 30
Val Gln Val Asp Tyr Cys Gly Ile Cys His Ser Asp Leu Ser Met Ile
35 40 45
Asp Asn Glu Trp Gly Phe Ser Gln Tyr Pro Leu Val Ala Gly His Glu
50 55 60
Val Ile Gly Arg Val Val Ala Leu Gly Ser Ala Ala Gln Asp Lys Gly
65 70 75 80
Leu Gln Val Gly Gln Arg Val Gly Ile Gly Trp Thr Ala Arg Ser Cys
85 90 95
Gly His Cys Asp Ala Cys Ile Ser Gly Asn Gln Ile Asn Cys Glu Gln
100 105 110
Gly Ala Val Pro Thr Ile Met Asn Arg Gly Gly Phe Ala Glu Lys Leu
115 120 125
Arg Ala Asp Trp Gln Trp Val Ile Pro Leu Pro Glu Asn Ile Asp Ile
130 135 140
Glu Ser Ala Gly Pro Leu Leu Cys Gly Gly Ile Thr Val Phe Lys Pro
145 150 155 160
Leu Leu Met His His Ile Thr Ala Thr Ser Arg Val Gly Val Ile Gly
165 170 175
Ile Gly Gly Leu Gly His Ile Ala Ile Lys Leu Leu His Ala Met Gly
180 185 190
Cys Glu Val Thr Ala Phe Ser Ser Asn Pro Ala Lys Glu Gln Glu Val
195 200 205
Leu Ala Met Gly Ala Asp Lys Val Val Asn Ser Arg Asp Pro Gln Ala
210 215 220
Leu Lys Ala Leu Ala Gly Gln Phe Asp Leu Ile Ile Asn Thr Val Asn
225 230 235 240
Val Ser Leu Asp Trp Gln Pro Tyr Phe Glu Ala Leu Thr Tyr Gly Gly
245 250 255
Asn Phe His Thr Val Gly Ala Val Leu Thr Pro Leu Ser Val Pro Ala
260 265 270
Phe Thr Leu Ile Ala Gly Asp Arg Ser Val Ser Gly Ser Ala Thr Gly
275 280 285
Thr Pro Tyr Glu Leu Arg Lys Leu Met Arg Phe Ala Ala Arg Ser Lys
290 295 300
Val Ala Pro Thr Thr Glu Leu Phe Pro Met Ser Lys Ile Asn Asp Ala
305 310 315 320
Ile Gln His Val Arg Asp Gly Lys Ala Arg Tyr Arg Val Val Leu Lys
325 330 335
Ala Asp Phe
<210> 15
<211> 1053
<212> DNA
<213> Unkown
<400> 15
atgaattatc agaacgacga tttacgcatc aaagaaatca aagagttact tcctcctgtc 60
gcattgctgg aaaaattccc cgctactgaa aatgccgcga atacggttgc ccatgcccga 120
aaagcgatcc ataagatcct gaaaggtaat gatgatcgcc tgttggttgt gattggccca 180
tgctcaattc atgatcctgt cgcggcaaaa gagtatgcca ctcgcttgct ggcgctgcgt 240
gaagagctga aagatgagct ggaaatcgta atgcgcgtct attttgaaaa gccgcgtacc 300
acggtgggct ggaaagggct gattaacgat ccgcatatgg ataatagctt ccagatcaac 360
gacggtctgc gtatagcccg taaattgctg cttgatatta acgacagcgg tctgccagcg 420
gcaggtgagt ttctcaatat gatcacccca caatatctcg ctgacctgat gagctggggc 480
gcaattggcg cacgtaccac cgaatcgcag gtgcaccgcg aactggcatc agggctttct 540
tgtccggtcg gcttcaaaaa tggcaccgac ggtacgatta aagtggctat cgatgccatt 600
aatgccgccg gtgcgccgca ctgcttcctg tccgtaacga aatgggggca ttcggcgatt 660
gtgaatacca gcggtaacgg cgattgccat atcattctgc gcggcggtaa agagcctaac 720
tacagcgcga agcacgttgc tgaagtgaaa gaagggctga acaaagcagg cctgccagca 780
caggtgatga tcgatttcag ccatgctaac tcgtccaaac aattcaaaaa gcagatggat 840
gtttgtgctg acgtttgcca gcagattgcc ggtggcgaaa aggccattat tggcgtgatg 900
gtggaaagcc atctggtgga aggcaatcag agcctcgaga gcggggagcc gctggcctac 960
ggtaagagca tcaccgatgc ctgcatcggc tgggaagata ccgatgctct gttacgtcaa 1020
ctggcgaatg cagtaaaagc gcgtcgcggg taa 1053
<210> 16
<211> 350
<212> PRT
<213> Unkown
<400> 16
Met Asn Tyr Gln Asn Asp Asp Leu Arg Ile Lys Glu Ile Lys Glu Leu
1 5 10 15
Leu Pro Pro Val Ala Leu Leu Glu Lys Phe Pro Ala Thr Glu Asn Ala
20 25 30
Ala Asn Thr Val Ala His Ala Arg Lys Ala Ile His Lys Ile Leu Lys
35 40 45
Gly Asn Asp Asp Arg Leu Leu Val Val Ile Gly Pro Cys Ser Ile His
50 55 60
Asp Pro Val Ala Ala Lys Glu Tyr Ala Thr Arg Leu Leu Ala Leu Arg
65 70 75 80
Glu Glu Leu Lys Asp Glu Leu Glu Ile Val Met Arg Val Tyr Phe Glu
85 90 95
Lys Pro Arg Thr Thr Val Gly Trp Lys Gly Leu Ile Asn Asp Pro His
100 105 110
Met Asp Asn Ser Phe Gln Ile Asn Asp Gly Leu Arg Ile Ala Arg Lys
115 120 125
Leu Leu Leu Asp Ile Asn Asp Ser Gly Leu Pro Ala Ala Gly Glu Phe
130 135 140
Leu Asn Met Ile Thr Pro Gln Tyr Leu Ala Asp Leu Met Ser Trp Gly
145 150 155 160
Ala Ile Gly Ala Arg Thr Thr Glu Ser Gln Val His Arg Glu Leu Ala
165 170 175
Ser Gly Leu Ser Cys Pro Val Gly Phe Lys Asn Gly Thr Asp Gly Thr
180 185 190
Ile Lys Val Ala Ile Asp Ala Ile Asn Ala Ala Gly Ala Pro His Cys
195 200 205
Phe Leu Ser Val Thr Lys Trp Gly His Ser Ala Ile Val Asn Thr Ser
210 215 220
Gly Asn Gly Asp Cys His Ile Ile Leu Arg Gly Gly Lys Glu Pro Asn
225 230 235 240
Tyr Ser Ala Lys His Val Ala Glu Val Lys Glu Gly Leu Asn Lys Ala
245 250 255
Gly Leu Pro Ala Gln Val Met Ile Asp Phe Ser His Ala Asn Ser Ser
260 265 270
Lys Gln Phe Lys Lys Gln Met Asp Val Cys Ala Asp Val Cys Gln Gln
275 280 285
Ile Ala Gly Gly Glu Lys Ala Ile Ile Gly Val Met Val Glu Ser His
290 295 300
Leu Val Glu Gly Asn Gln Ser Leu Glu Ser Gly Glu Pro Leu Ala Tyr
305 310 315 320
Gly Lys Ser Ile Thr Asp Ala Cys Ile Gly Trp Glu Asp Thr Asp Ala
325 330 335
Leu Leu Arg Gln Leu Ala Asn Ala Val Lys Ala Arg Arg Gly
340 345 350
<210> 17
<211> 1122
<212> DNA
<213> Unkown
<400> 17
atggttgctg aattgaccgc attacgcgat caaattgatg aagtcgataa agcgctgctg 60
aatttattag cgaagcgtct ggaactggtt gctgaagtgg gcgaggtgaa aagccgcttt 120
ggactgccta tttatgttcc ggagcgcgag gcatctattt tggcctcgcg gcgcgcagag 180
gcggaagctc tgggtgtacc gccagatctg attgaggatg ttttgcgtcg ggtgatgcgt 240
gaatcttact ccagtgaaaa cgacaaagga tttaaaacac tttgtccgtc actgcgtccg 300
gtggttatcg tcggcggtgg cggtcagatg ggacgcctgt tcgagaagat gctgacacta 360
tcgggttatc aggtgcggat tctggagcaa catgactggg atcgagcggc tgatattgtt 420
gccgatgccg gaatggtgat tgttagtgtg ccaatccacg ttactgagca agttatcggc 480
aaattaccgc ctttaccgaa agattgtatt ctggttgatc tggcatcagt gaaaaatgga 540
ccattacagg ccatgctggc ggcgcacgat ggcccggtac tggggttaca cccgatgttc 600
ggcccggaca gcggtagcct ggcaaagcaa gttgtggtct ggtgtgatgg acgtaagccg 660
gaagcatacc aatggtttct ggagcaaatt caggtctggg gcgctcggct gcatcgtatt 720
agcgctgtcg agcacgatca gaatatggcg tttattcagg ctctgcgcca ctttgctact 780
tttgcttatg ggctgcatct ggcagaagaa aatgttcagc ttgagcaact tctggcgctc 840
tcttcgccga tttaccgcct tgagctggcg atggtcgggc gactgtttgc tcaggatccg 900
cagctttatg ccgacattat tatgtcgtca gagcgtaatc tggcgttaat caaacgttac 960
tataagcgtt tcggcgaggc gattgagttg ctggagcagg gcgataagca ggcgtttatt 1020
gacagtttcc gcaaggtgga gcactggttc ggcgattacg ttcagcgttt tcagagtgaa 1080
agccgcgtgt tattgcgtca ggcgaatgac aatcgccagt aa 1122
<210> 18
<211> 373
<212> PRT
<213> Unkown
<400> 18
Met Val Ala Glu Leu Thr Ala Leu Arg Asp Gln Ile Asp Glu Val Asp
1 5 10 15
Lys Ala Leu Leu Asn Leu Leu Ala Lys Arg Leu Glu Leu Val Ala Glu
20 25 30
Val Gly Glu Val Lys Ser Arg Phe Gly Leu Pro Ile Tyr Val Pro Glu
35 40 45
Arg Glu Ala Ser Ile Leu Ala Ser Arg Arg Ala Glu Ala Glu Ala Leu
50 55 60
Gly Val Pro Pro Asp Leu Ile Glu Asp Val Leu Arg Arg Val Met Arg
65 70 75 80
Glu Ser Tyr Ser Ser Glu Asn Asp Lys Gly Phe Lys Thr Leu Cys Pro
85 90 95
Ser Leu Arg Pro Val Val Ile Val Gly Gly Gly Gly Gln Met Gly Arg
100 105 110
Leu Phe Glu Lys Met Leu Thr Leu Ser Gly Tyr Gln Val Arg Ile Leu
115 120 125
Glu Gln His Asp Trp Asp Arg Ala Ala Asp Ile Val Ala Asp Ala Gly
130 135 140
Met Val Ile Val Ser Val Pro Ile His Val Thr Glu Gln Val Ile Gly
145 150 155 160
Lys Leu Pro Pro Leu Pro Lys Asp Cys Ile Leu Val Asp Leu Ala Ser
165 170 175
Val Lys Asn Gly Pro Leu Gln Ala Met Leu Ala Ala His Asp Gly Pro
180 185 190
Val Leu Gly Leu His Pro Met Phe Gly Pro Asp Ser Gly Ser Leu Ala
195 200 205
Lys Gln Val Val Val Trp Cys Asp Gly Arg Lys Pro Glu Ala Tyr Gln
210 215 220
Trp Phe Leu Glu Gln Ile Gln Val Trp Gly Ala Arg Leu His Arg Ile
225 230 235 240
Ser Ala Val Glu His Asp Gln Asn Met Ala Phe Ile Gln Ala Leu Arg
245 250 255
His Phe Ala Thr Phe Ala Tyr Gly Leu His Leu Ala Glu Glu Asn Val
260 265 270
Gln Leu Glu Gln Leu Leu Ala Leu Ser Ser Pro Ile Tyr Arg Leu Glu
275 280 285
Leu Ala Met Val Gly Arg Leu Phe Ala Gln Asp Pro Gln Leu Tyr Ala
290 295 300
Asp Ile Ile Met Ser Ser Glu Arg Asn Leu Ala Leu Ile Lys Arg Tyr
305 310 315 320
Tyr Lys Arg Phe Gly Glu Ala Ile Glu Leu Leu Glu Gln Gly Asp Lys
325 330 335
Gln Ala Phe Ile Asp Ser Phe Arg Lys Val Glu His Trp Phe Gly Asp
340 345 350
Tyr Val Gln Arg Phe Gln Ser Glu Ser Arg Val Leu Leu Arg Gln Ala
355 360 365
Asn Asp Asn Arg Gln
370
<210> 19
<211> 1908
<212> DNA
<213> Unkown
<400> 19
atggcacctg ttacaattga aaagttcgta aatcaagaag aacgacacct tgtttccaac 60
cgatcagcaa caattccgtt tggtgaatac atatttaaaa gattgttgtc catcgatacg 120
aaatcagttt tcggtgttcc tggtgacttc aacttatctc tattagaata tctctattca 180
cctagtgttg aatcagctgg cctaagatgg gtcggcacgt gtaatgaact gaacgccgct 240
tatgcggccg acggatattc ccgttactct aataagattg gctgtttaat aaccacgtat 300
ggcgttggtg aattaagcgc cttgaacggt atagccggtt cgttcgctga aaatgtcaaa 360
gttttgcaca ttgttggtgt ggccaagtcc atagattcgc gttcaagtaa ctttagtgat 420
cggaacctac atcatttggt cccacagcta catgattcaa attttaaagg gccaaatcat 480
aaagtatatc atgatatggt aaaagataga gtcgcttgct cggtagccta cttggaggat 540
attgaaactg catgtgacca agtcgataat gttatccgcg atatttacaa gtattctaaa 600
cctggttata tttttgttcc tgcagatttt gcggatatgt ctgttacatg tgataatttg 660
gttaatgttc cacgtatatc tcaacaagat tgtatagtat acccttctga aaaccaattg 720
tctgacataa tcaacaagat tactagttgg atatattcca gtaaaacacc tgcgatcctt 780
ggagacgtac tgactgatag gtatggtgtg agtaactttt tgaacaagct tatctgcaaa 840
actgggattt ggaatttttc cactgttatg ggaaaatctg taattgatga gtcaaaccca 900
acttatatgg gtcaatataa tggtaaagaa ggtttaaaac aagtctatga acattttgaa 960
ctgtgcgact tggtcttgca ttttggagtc gacatcaatg aaattaataa tgggcattat 1020
acttttactt ataaaccaaa tgctaaaatc attcaatttc atccgaatta tattcgcctt 1080
gtggacacta ggcagggcaa tgagcaaatg ttcaaaggaa tcaattttgc ccctatttta 1140
aaagaactat acaagcgcat tgacgtttct aaactttctt tgcaatatga ttcaaatgta 1200
actcaatata cgaacgaaac aatgcggtta gaagatccta ccaatggaca atcaagcatt 1260
attacacaag ttcacttaca aaagacgatg cctaaatttt tgaaccctgg tgatgttgtc 1320
gtttgtgaaa caggctcttt tcaattctct gttcgtgatt tcgcgtttcc ttcgcaatta 1380
aaatatatat cgcaaggatt tttcctttcc attggcatgg cccttcctgc cgccctaggt 1440
gttggaattg ccatgcaaga ccactcaaac gctcacatca atggtggcaa cgtaaaagag 1500
gactataagc caagattaat tttgtttgaa ggtgacggtg cagcacagat gacaatccaa 1560
gaactgagca ccattctgaa gtgcaatatt ccactagaag ttatcatttg gaacaataac 1620
ggctacacta ttgaaagagc catcatgggc cctaccaggt cgtataacga cgttatgtct 1680
tggaaatgga ccaaactatt tgaagcattc ggagacttcg acggaaagta tactaatagc 1740
actctcattc aatgtccctc taaattagca ctgaaattgg aggagcttaa gaattcaaac 1800
aaaagaagcg ggatagaact tttagaagtc aaattaggcg aattggattt ccccgaacag 1860
ctaaagtgca tggttgaagc agcggcactt aaaagaaata aaaaatag 1908
<210> 20
<211> 635
<212> PRT
<213> Unkown
<400> 20
Met Ala Pro Val Thr Ile Glu Lys Phe Val Asn Gln Glu Glu Arg His
1 5 10 15
Leu Val Ser Asn Arg Ser Ala Thr Ile Pro Phe Gly Glu Tyr Ile Phe
20 25 30
Lys Arg Leu Leu Ser Ile Asp Thr Lys Ser Val Phe Gly Val Pro Gly
35 40 45
Asp Phe Asn Leu Ser Leu Leu Glu Tyr Leu Tyr Ser Pro Ser Val Glu
50 55 60
Ser Ala Gly Leu Arg Trp Val Gly Thr Cys Asn Glu Leu Asn Ala Ala
65 70 75 80
Tyr Ala Ala Asp Gly Tyr Ser Arg Tyr Ser Asn Lys Ile Gly Cys Leu
85 90 95
Ile Thr Thr Tyr Gly Val Gly Glu Leu Ser Ala Leu Asn Gly Ile Ala
100 105 110
Gly Ser Phe Ala Glu Asn Val Lys Val Leu His Ile Val Gly Val Ala
115 120 125
Lys Ser Ile Asp Ser Arg Ser Ser Asn Phe Ser Asp Arg Asn Leu His
130 135 140
His Leu Val Pro Gln Leu His Asp Ser Asn Phe Lys Gly Pro Asn His
145 150 155 160
Lys Val Tyr His Asp Met Val Lys Asp Arg Val Ala Cys Ser Val Ala
165 170 175
Tyr Leu Glu Asp Ile Glu Thr Ala Cys Asp Gln Val Asp Asn Val Ile
180 185 190
Arg Asp Ile Tyr Lys Tyr Ser Lys Pro Gly Tyr Ile Phe Val Pro Ala
195 200 205
Asp Phe Ala Asp Met Ser Val Thr Cys Asp Asn Leu Val Asn Val Pro
210 215 220
Arg Ile Ser Gln Gln Asp Cys Ile Val Tyr Pro Ser Glu Asn Gln Leu
225 230 235 240
Ser Asp Ile Ile Asn Lys Ile Thr Ser Trp Ile Tyr Ser Ser Lys Thr
245 250 255
Pro Ala Ile Leu Gly Asp Val Leu Thr Asp Arg Tyr Gly Val Ser Asn
260 265 270
Phe Leu Asn Lys Leu Ile Cys Lys Thr Gly Ile Trp Asn Phe Ser Thr
275 280 285
Val Met Gly Lys Ser Val Ile Asp Glu Ser Asn Pro Thr Tyr Met Gly
290 295 300
Gln Tyr Asn Gly Lys Glu Gly Leu Lys Gln Val Tyr Glu His Phe Glu
305 310 315 320
Leu Cys Asp Leu Val Leu His Phe Gly Val Asp Ile Asn Glu Ile Asn
325 330 335
Asn Gly His Tyr Thr Phe Thr Tyr Lys Pro Asn Ala Lys Ile Ile Gln
340 345 350
Phe His Pro Asn Tyr Ile Arg Leu Val Asp Thr Arg Gln Gly Asn Glu
355 360 365
Gln Met Phe Lys Gly Ile Asn Phe Ala Pro Ile Leu Lys Glu Leu Tyr
370 375 380
Lys Arg Ile Asp Val Ser Lys Leu Ser Leu Gln Tyr Asp Ser Asn Val
385 390 395 400
Thr Gln Tyr Thr Asn Glu Thr Met Arg Leu Glu Asp Pro Thr Asn Gly
405 410 415
Gln Ser Ser Ile Ile Thr Gln Val His Leu Gln Lys Thr Met Pro Lys
420 425 430
Phe Leu Asn Pro Gly Asp Val Val Val Cys Glu Thr Gly Ser Phe Gln
435 440 445
Phe Ser Val Arg Asp Phe Ala Phe Pro Ser Gln Leu Lys Tyr Ile Ser
450 455 460
Gln Gly Phe Phe Leu Ser Ile Gly Met Ala Leu Pro Ala Ala Leu Gly
465 470 475 480
Val Gly Ile Ala Met Gln Asp His Ser Asn Ala His Ile Asn Gly Gly
485 490 495
Asn Val Lys Glu Asp Tyr Lys Pro Arg Leu Ile Leu Phe Glu Gly Asp
500 505 510
Gly Ala Ala Gln Met Thr Ile Gln Glu Leu Ser Thr Ile Leu Lys Cys
515 520 525
Asn Ile Pro Leu Glu Val Ile Ile Trp Asn Asn Asn Gly Tyr Thr Ile
530 535 540
Glu Arg Ala Ile Met Gly Pro Thr Arg Ser Tyr Asn Asp Val Met Ser
545 550 555 560
Trp Lys Trp Thr Lys Leu Phe Glu Ala Phe Gly Asp Phe Asp Gly Lys
565 570 575
Tyr Thr Asn Ser Thr Leu Ile Gln Cys Pro Ser Lys Leu Ala Leu Lys
580 585 590
Leu Glu Glu Leu Lys Asn Ser Asn Lys Arg Ser Gly Ile Glu Leu Leu
595 600 605
Glu Val Lys Leu Gly Glu Leu Asp Phe Pro Glu Gln Leu Lys Cys Met
610 615 620
Val Glu Ala Ala Ala Leu Lys Arg Asn Lys Lys
625 630 635
<210> 21
<211> 1419
<212> DNA
<213> Unkown
<400> 21
atgacccccg aacagttccg tcagtatggc caccagctga tcgatttaat cgcagattat 60
cgccagaccg ttggtgaacg tccggttatg gcacaagttg aaccgggcta tctgaaagca 120
gctttaccgg caacagcacc tcaacaaggt gaacctttcg ccgccatttt agatgatgtg 180
aacaatctgg tgatgccggg tctgagccac tggcagcatc cggacttcta tggctacttt 240
ccgagcaatg gtactttaag cagtgtgctg ggcgactttc tgagtaccgg tctgggtgtt 300
ctgggtttaa gttggcagag cagcccggca ctgagcgaac tggaggaaac aacactggac 360
tggctgcgtc agctgctggg tctgagcggt cagtggagcg gtgtgatcca agatacagcc 420
agcaccagta ctttagtggc tttaattagc gcacgtgaac gcgccaccga ttacgcactg 480
gttcgtggtg gtttacaagc tgaaccgaaa ccgctgatcg tgtacgtgag cgcacatgcc 540
catagcagcg tggataaagc cgcactgctg gccggttttg gccgcgataa tattcgttta 600
attccgaccg acgaacgtta cgcactgcgt ccggaagctt tacaagctgc aattgagcaa 660
gatctggccg ccggcaatca gccgtgcgca gttgttgcaa ccaccggtac caccaccaca 720
accgcattag atccgctgcg tcccgttggc gaaatcgcac aagctaatgg tttatggctg 780
cacgtggaca gcgcaatggc tggtagcgcc atgattctgc cggaatgccg ttggatgtgg 840
gacggtatcg agttagcaga cagcgttgtg gtgaacgcac acaaatggct gggtgtggcc 900
ttcgactgca gcatctatta cgtgcgcgat ccgcagcact taatccgcgt gatgagcacc 960
aatccgagct atctgcagag cgcagtggac ggtgaggtga aaaatttacg tgattggggc 1020
attccgctgg gccgtcgttt ccgtgcttta aaactgtggt tcatgctgcg cagtgaaggc 1080
gtggatgcac tgcaagctcg tctgcgtcgt gatctggata atgcacagtg gctggctggt 1140
caagttgaag cagcagcaga atgggaggtg ctggccccgg ttcaactgca gactttatgc 1200
attcgccacc gtcccgctgg tctggaaggc gaggcactgg atgcccatac caaaggttgg 1260
gccgaacgtt taaatgcaag cggtgccgca tacgtgaccc cggcaacttt agatggtcgc 1320
tggatggtgc gtgttagcat tggtgcttta ccgaccgaac gtggtgatgt tcagcgtctg 1380
tgggcacgtt tacaagacgt gattaaaggc ggcagctaa 1419
<210> 22
<211> 472
<212> PRT
<213> Unkown
<400> 22
Met Thr Pro Glu Gln Phe Arg Gln Tyr Gly His Gln Leu Ile Asp Leu
1 5 10 15
Ile Ala Asp Tyr Arg Gln Thr Val Gly Glu Arg Pro Val Met Ala Gln
20 25 30
Val Glu Pro Gly Tyr Leu Lys Ala Ala Leu Pro Ala Thr Ala Pro Gln
35 40 45
Gln Gly Glu Pro Phe Ala Ala Ile Leu Asp Asp Val Asn Asn Leu Val
50 55 60
Met Pro Gly Leu Ser His Trp Gln His Pro Asp Phe Tyr Gly Tyr Phe
65 70 75 80
Pro Ser Asn Gly Thr Leu Ser Ser Val Leu Gly Asp Phe Leu Ser Thr
85 90 95
Gly Leu Gly Val Leu Gly Leu Ser Trp Gln Ser Ser Pro Ala Leu Ser
100 105 110
Glu Leu Glu Glu Thr Thr Leu Asp Trp Leu Arg Gln Leu Leu Gly Leu
115 120 125
Ser Gly Gln Trp Ser Gly Val Ile Gln Asp Thr Ala Ser Thr Ser Thr
130 135 140
Leu Val Ala Leu Ile Ser Ala Arg Glu Arg Ala Thr Asp Tyr Ala Leu
145 150 155 160
Val Arg Gly Gly Leu Gln Ala Glu Pro Lys Pro Leu Ile Val Tyr Val
165 170 175
Ser Ala His Ala His Ser Ser Val Asp Lys Ala Ala Leu Leu Ala Gly
180 185 190
Phe Gly Arg Asp Asn Ile Arg Leu Ile Pro Thr Asp Glu Arg Tyr Ala
195 200 205
Leu Arg Pro Glu Ala Leu Gln Ala Ala Ile Glu Gln Asp Leu Ala Ala
210 215 220
Gly Asn Gln Pro Cys Ala Val Val Ala Thr Thr Gly Thr Thr Thr Thr
225 230 235 240
Thr Ala Leu Asp Pro Leu Arg Pro Val Gly Glu Ile Ala Gln Ala Asn
245 250 255
Gly Leu Trp Leu His Val Asp Ser Ala Met Ala Gly Ser Ala Met Ile
260 265 270
Leu Pro Glu Cys Arg Trp Met Trp Asp Gly Ile Glu Leu Ala Asp Ser
275 280 285
Val Val Val Asn Ala His Lys Trp Leu Gly Val Ala Phe Asp Cys Ser
290 295 300
Ile Tyr Tyr Val Arg Asp Pro Gln His Leu Ile Arg Val Met Ser Thr
305 310 315 320
Asn Pro Ser Tyr Leu Gln Ser Ala Val Asp Gly Glu Val Lys Asn Leu
325 330 335
Arg Asp Trp Gly Ile Pro Leu Gly Arg Arg Phe Arg Ala Leu Lys Leu
340 345 350
Trp Phe Met Leu Arg Ser Glu Gly Val Asp Ala Leu Gln Ala Arg Leu
355 360 365
Arg Arg Asp Leu Asp Asn Ala Gln Trp Leu Ala Gly Gln Val Glu Ala
370 375 380
Ala Ala Glu Trp Glu Val Leu Ala Pro Val Gln Leu Gln Thr Leu Cys
385 390 395 400
Ile Arg His Arg Pro Ala Gly Leu Glu Gly Glu Ala Leu Asp Ala His
405 410 415
Thr Lys Gly Trp Ala Glu Arg Leu Asn Ala Ser Gly Ala Ala Tyr Val
420 425 430
Thr Pro Ala Thr Leu Asp Gly Arg Trp Met Val Arg Val Ser Ile Gly
435 440 445
Ala Leu Pro Thr Glu Arg Gly Asp Val Gln Arg Leu Trp Ala Arg Leu
450 455 460
Gln Asp Val Ile Lys Gly Gly Ser
465 470
<210> 23
<211> 609
<212> DNA
<213> Unkown
<400> 23
atgcgtaaag tgatcaaata tgatatggag gtggccgtga gcgccgatag cgtttgggca 60
gtgtatagta gcccggatat cccgcgtctg ctgcgcgatg ttctgctgcc gggtgtgttc 120
gagaagctgg acgtgatcga aggcaatggt ggtgttggca ccgttctgga cattgtgttt 180
ccgccgggcg cagttccgcg cagctataaa gaaaaatttg tgaacattga tcgcgaaaag 240
cgtttaaaag aagttatcat gatcgaaggc ggttatttag atatgggctg caccttctat 300
ctggatcgca ttcacgtggt ggagaaaacc aagagcagct gcgtgatcga gagcagcatt 360
gtgtacgacg tgaaagagga gtgtgccgat gccatgagca agctgattac caccgaaccg 420
ctgaaaagca tggccgaagt gattagcaat tatgtgattc agaaagagct gttcagcgcc 480
cgcaacattt taagcaagca gagcgtggtg aaaaaggaga tccgctatga tttagaggtg 540
ccgattagcg tggacagcat ttggagcgtg tacagctgcc cggatattcc tcgtttactg 600
ggcagctaa 609
<210> 24
<211> 202
<212> PRT
<213> Unkown
<400> 24
Met Arg Lys Val Ile Lys Tyr Asp Met Glu Val Ala Val Ser Ala Asp
1 5 10 15
Ser Val Trp Ala Val Tyr Ser Ser Pro Asp Ile Pro Arg Leu Leu Arg
20 25 30
Asp Val Leu Leu Pro Gly Val Phe Glu Lys Leu Asp Val Ile Glu Gly
35 40 45
Asn Gly Gly Val Gly Thr Val Leu Asp Ile Val Phe Pro Pro Gly Ala
50 55 60
Val Pro Arg Ser Tyr Lys Glu Lys Phe Val Asn Ile Asp Arg Glu Lys
65 70 75 80
Arg Leu Lys Glu Val Ile Met Ile Glu Gly Gly Tyr Leu Asp Met Gly
85 90 95
Cys Thr Phe Tyr Leu Asp Arg Ile His Val Val Glu Lys Thr Lys Ser
100 105 110
Ser Cys Val Ile Glu Ser Ser Ile Val Tyr Asp Val Lys Glu Glu Cys
115 120 125
Ala Asp Ala Met Ser Lys Leu Ile Thr Thr Glu Pro Leu Lys Ser Met
130 135 140
Ala Glu Val Ile Ser Asn Tyr Val Ile Gln Lys Glu Leu Phe Ser Ala
145 150 155 160
Arg Asn Ile Leu Ser Lys Gln Ser Val Val Lys Lys Glu Ile Arg Tyr
165 170 175
Asp Leu Glu Val Pro Ile Ser Val Asp Ser Ile Trp Ser Val Tyr Ser
180 185 190
Cys Pro Asp Ile Pro Arg Leu Leu Gly Ser
195 200
<210> 25
<211> 1041
<212> DNA
<213> Unkown
<400> 25
atggagaccg ttagcaaaat tgaccaacag aatcaagcta aaatctggaa gcagatctat 60
ggcttcgccg aatctttagt tctgaaatgt gccgtgcagc tggaaatcgc cgagacactg 120
cacaacaacg tgaaaccgat gtctttaagc gaactggcca gcaagctgcc ggttgcacag 180
ccggttaacg aagaccgttt attccgcatc atgcgctatt tagtgcatat ggaactgttc 240
aaaattgatg ccaccaccca gaaatactct ttagccccgc cggccaaata tttactgcgc 300
ggctgggaga aaagcatggt ggatagcatt ctgtgcatca acgataagga ctttctggcc 360
ccttggcacc atctgggtga tggtctgacc ggtaattgcg atgcattcga gaaagcttta 420
ggcaagagca tttgggtgta tatgagcgaa aacccggaga aaaaccagct gttcaacgcc 480
gcaatggcat gcgatacccg tttagtgaca agcgcactgg ccaacgagtg caagagcatc 540
tttagcgacg gcattagcac tttagttgat gttggcggcg gtaccggtac cgcagtgaag 600
gcaatcagca aagcattccc ggacattaaa tgcaccattt atgatttacc gcatgtgatc 660
gccgatagcc cggagattcc gaacatcacc aagatcagtg gcgacatgtt caaaagtatc 720
ccgagcgccg acgccatctt tatgaagtgc attttacacg actggaatga cgacgaatgc 780
attcagattt taaaacgctg caaagaagct ttaccgaaag ttggcaaggt gatcatcgtg 840
gacgtggtta tcgacatgga cagcacccat ccgtatgcca aaatccgttt aactttagat 900
ctggacatga tgctgaacac cggcggcaaa gaacgcacca aagaagaatg gaaaacttta 960
ttcgatgcag ccggttttgc cagccacaaa gtgacacaga ttagcgccgt gcagagcgtg 1020
attgaagcat atccgtacta a 1041
<210> 26
<211> 346
<212> PRT
<213> Unkown
<400> 26
Met Glu Thr Val Ser Lys Ile Asp Gln Gln Asn Gln Ala Lys Ile Trp
1 5 10 15
Lys Gln Ile Tyr Gly Phe Ala Glu Ser Leu Val Leu Lys Cys Ala Val
20 25 30
Gln Leu Glu Ile Ala Glu Thr Leu His Asn Asn Val Lys Pro Met Ser
35 40 45
Leu Ser Glu Leu Ala Ser Lys Leu Pro Val Ala Gln Pro Val Asn Glu
50 55 60
Asp Arg Leu Phe Arg Ile Met Arg Tyr Leu Val His Met Glu Leu Phe
65 70 75 80
Lys Ile Asp Ala Thr Thr Gln Lys Tyr Ser Leu Ala Pro Pro Ala Lys
85 90 95
Tyr Leu Leu Arg Gly Trp Glu Lys Ser Met Val Asp Ser Ile Leu Cys
100 105 110
Ile Asn Asp Lys Asp Phe Leu Ala Pro Trp His His Leu Gly Asp Gly
115 120 125
Leu Thr Gly Asn Cys Asp Ala Phe Glu Lys Ala Leu Gly Lys Ser Ile
130 135 140
Trp Val Tyr Met Ser Glu Asn Pro Glu Lys Asn Gln Leu Phe Asn Ala
145 150 155 160
Ala Met Ala Cys Asp Thr Arg Leu Val Thr Ser Ala Leu Ala Asn Glu
165 170 175
Cys Lys Ser Ile Phe Ser Asp Gly Ile Ser Thr Leu Val Asp Val Gly
180 185 190
Gly Gly Thr Gly Thr Ala Val Lys Ala Ile Ser Lys Ala Phe Pro Asp
195 200 205
Ile Lys Cys Thr Ile Tyr Asp Leu Pro His Val Ile Ala Asp Ser Pro
210 215 220
Glu Ile Pro Asn Ile Thr Lys Ile Ser Gly Asp Met Phe Lys Ser Ile
225 230 235 240
Pro Ser Ala Asp Ala Ile Phe Met Lys Cys Ile Leu His Asp Trp Asn
245 250 255
Asp Asp Glu Cys Ile Gln Ile Leu Lys Arg Cys Lys Glu Ala Leu Pro
260 265 270
Lys Val Gly Lys Val Ile Ile Val Asp Val Val Ile Asp Met Asp Ser
275 280 285
Thr His Pro Tyr Ala Lys Ile Arg Leu Thr Leu Asp Leu Asp Met Met
290 295 300
Leu Asn Thr Gly Gly Lys Glu Arg Thr Lys Glu Glu Trp Lys Thr Leu
305 310 315 320
Phe Asp Ala Ala Gly Phe Ala Ser His Lys Val Thr Gln Ile Ser Ala
325 330 335
Val Gln Ser Val Ile Glu Ala Tyr Pro Tyr
340 345
<210> 27
<211> 1053
<212> DNA
<213> Unkown
<400> 27
atggccttcc atggcaagga cgacgtgctg gatattaagg cccaagctca tgtgtggaag 60
atcatttacg gcttcgctga ttctttagtg ctgcgttgcg ccgtggagtt aggcatcgtg 120
gatatcattg ataacaataa ccaaccgatg gctttagccg atctggccag caaactgccg 180
gttagcgacg tgaactgcga taatctgtac cgcattctgc gctatttagt taagatggag 240
attttacgcg tggagaaaag cgatgacggc cagaaaaagt atgctttaga accgattgcc 300
actttactga gtcgcaatgc caaacgtagc atggtgccga tgattttagg catgacccag 360
aaggatttca tgaccccgtg gcacagcatg aaagatggtt taagcgataa tggcaccgcc 420
tttgagaagg caatgggcat gaccatctgg gaatatttag aaggtcaccc ggatcagagc 480
cagctgttca atgagggcat ggctggtgaa acccgtttac tgaccagcag tctgatcagc 540
ggtagccgcg acatgtttca aggtatcgat tctctggtgg atgttggtgg tggcaatggt 600
accaccgtta aggccattag cgacgccttc ccgcacatca aatgcacact gttcgattta 660
cctcacgtga tcgccaacag ctacgatctg ccgaacattg aacgcatcgg tggcgatatg 720
tttaaaagcg tgccgagcgc ccaagctatc attttaaagc tgattttaca tgactggaac 780
gatgaggaca gcatcaagat tttaaagcag tgccgcaacg ccgtgccgaa agatggcggc 840
aaagtgatca tcgtggatgt ggctttagac gaggaaagcg atcatgagct gagtagcacc 900
cgtctgattt tagacatcga tatgctggtg aataccggcg gtaaagagcg taccaaggaa 960
gtttgggaaa aaatcgtgaa gagcgccggc tttagcggct gcaaaattcg ccacattgcc 1020
gccatccaga gcgtgattga ggtgtttcct taa 1053
<210> 28
<211> 350
<212> PRT
<213> Unkown
<400> 28
Met Ala Phe His Gly Lys Asp Asp Val Leu Asp Ile Lys Ala Gln Ala
1 5 10 15
His Val Trp Lys Ile Ile Tyr Gly Phe Ala Asp Ser Leu Val Leu Arg
20 25 30
Cys Ala Val Glu Leu Gly Ile Val Asp Ile Ile Asp Asn Asn Asn Gln
35 40 45
Pro Met Ala Leu Ala Asp Leu Ala Ser Lys Leu Pro Val Ser Asp Val
50 55 60
Asn Cys Asp Asn Leu Tyr Arg Ile Leu Arg Tyr Leu Val Lys Met Glu
65 70 75 80
Ile Leu Arg Val Glu Lys Ser Asp Asp Gly Gln Lys Lys Tyr Ala Leu
85 90 95
Glu Pro Ile Ala Thr Leu Leu Ser Arg Asn Ala Lys Arg Ser Met Val
100 105 110
Pro Met Ile Leu Gly Met Thr Gln Lys Asp Phe Met Thr Pro Trp His
115 120 125
Ser Met Lys Asp Gly Leu Ser Asp Asn Gly Thr Ala Phe Glu Lys Ala
130 135 140
Met Gly Met Thr Ile Trp Glu Tyr Leu Glu Gly His Pro Asp Gln Ser
145 150 155 160
Gln Leu Phe Asn Glu Gly Met Ala Gly Glu Thr Arg Leu Leu Thr Ser
165 170 175
Ser Leu Ile Ser Gly Ser Arg Asp Met Phe Gln Gly Ile Asp Ser Leu
180 185 190
Val Asp Val Gly Gly Gly Asn Gly Thr Thr Val Lys Ala Ile Ser Asp
195 200 205
Ala Phe Pro His Ile Lys Cys Thr Leu Phe Asp Leu Pro His Val Ile
210 215 220
Ala Asn Ser Tyr Asp Leu Pro Asn Ile Glu Arg Ile Gly Gly Asp Met
225 230 235 240
Phe Lys Ser Val Pro Ser Ala Gln Ala Ile Ile Leu Lys Leu Ile Leu
245 250 255
His Asp Trp Asn Asp Glu Asp Ser Ile Lys Ile Leu Lys Gln Cys Arg
260 265 270
Asn Ala Val Pro Lys Asp Gly Gly Lys Val Ile Ile Val Asp Val Ala
275 280 285
Leu Asp Glu Glu Ser Asp His Glu Leu Ser Ser Thr Arg Leu Ile Leu
290 295 300
Asp Ile Asp Met Leu Val Asn Thr Gly Gly Lys Glu Arg Thr Lys Glu
305 310 315 320
Val Trp Glu Lys Ile Val Lys Ser Ala Gly Phe Ser Gly Cys Lys Ile
325 330 335
Arg His Ile Ala Ala Ile Gln Ser Val Ile Glu Val Phe Pro
340 345 350
<210> 29
<211> 1056
<212> DNA
<213> Unkown
<400> 29
atgcagttaa aagaaaaaga ggagctgctg cgcaacatgg aactgggtct gatcccggat 60
caagaaattc gccagcgtat ccgcatcgag ctggaaaaac gtttacagtg gggctacaaa 120
gagacccatg aggaacagct gagtcagctg ctggatttag tgcactcttt aaagggcatg 180
aaaatggcca ccgaaatgga gaatctggat ttaaagctgt atgaagcccc gatggagttt 240
ttaaagatcc agcacggtag caacatgaag cagagcgctg gttactatac cgacgaaagc 300
accactttag atgaagccga gattgccatg ctggatctgt acatggaacg cgcacagatc 360
aaggatggcc agagcgtttt agatttaggc tgcggcttag gcgccgttgc actgtttggc 420
gccaataaat ttaagaagtg ccagtttacc ggtgttacca gcagtgtgga gcagaaagac 480
tatattgaag gcaaatgcaa agaactgaaa ctgaccaacg tgaaagtgct gctggcagac 540
atcaccacct atgaaaccga ggagcgcttt gatcgcatct ttgccgtgga gctgatcgag 600
catatgaaaa actatcagct gttactgaag aaaattagcg agtggatgaa ggatgatggt 660
ttactgtttg tggaacacgt gtgccacaag actttagcct atcattacga accggtggac 720
gcagaggatt ggtacaccaa ctatattttc ccggccggca cactgacttt aagcagtgcc 780
agcatgctgc tgtatttcca agatgacgtt agcgtggtta accagtggac tttaagtggc 840
aagcactatt ctcgcagcca cgaagaatgg ctgaaaaaca tggataagaa tattgtggaa 900
tttaaggaaa ttatgcgtag tatcaccaaa accgaaaaag aagccattaa actgttaaac 960
ttctggcgta ttttctgcat gtgcggcgcc gagctgtttg gctataaaaa cggcgaggag 1020
tggatgttaa cccatctgct gtttaagaaa aaataa 1056
<210> 30
<211> 351
<212> PRT
<213> Unkown
<400> 30
Met Gln Leu Lys Glu Lys Glu Glu Leu Leu Arg Asn Met Glu Leu Gly
1 5 10 15
Leu Ile Pro Asp Gln Glu Ile Arg Gln Arg Ile Arg Ile Glu Leu Glu
20 25 30
Lys Arg Leu Gln Trp Gly Tyr Lys Glu Thr His Glu Glu Gln Leu Ser
35 40 45
Gln Leu Leu Asp Leu Val His Ser Leu Lys Gly Met Lys Met Ala Thr
50 55 60
Glu Met Glu Asn Leu Asp Leu Lys Leu Tyr Glu Ala Pro Met Glu Phe
65 70 75 80
Leu Lys Ile Gln His Gly Ser Asn Met Lys Gln Ser Ala Gly Tyr Tyr
85 90 95
Thr Asp Glu Ser Thr Thr Leu Asp Glu Ala Glu Ile Ala Met Leu Asp
100 105 110
Leu Tyr Met Glu Arg Ala Gln Ile Lys Asp Gly Gln Ser Val Leu Asp
115 120 125
Leu Gly Cys Gly Leu Gly Ala Val Ala Leu Phe Gly Ala Asn Lys Phe
130 135 140
Lys Lys Cys Gln Phe Thr Gly Val Thr Ser Ser Val Glu Gln Lys Asp
145 150 155 160
Tyr Ile Glu Gly Lys Cys Lys Glu Leu Lys Leu Thr Asn Val Lys Val
165 170 175
Leu Leu Ala Asp Ile Thr Thr Tyr Glu Thr Glu Glu Arg Phe Asp Arg
180 185 190
Ile Phe Ala Val Glu Leu Ile Glu His Met Lys Asn Tyr Gln Leu Leu
195 200 205
Leu Lys Lys Ile Ser Glu Trp Met Lys Asp Asp Gly Leu Leu Phe Val
210 215 220
Glu His Val Cys His Lys Thr Leu Ala Tyr His Tyr Glu Pro Val Asp
225 230 235 240
Ala Glu Asp Trp Tyr Thr Asn Tyr Ile Phe Pro Ala Gly Thr Leu Thr
245 250 255
Leu Ser Ser Ala Ser Met Leu Leu Tyr Phe Gln Asp Asp Val Ser Val
260 265 270
Val Asn Gln Trp Thr Leu Ser Gly Lys His Tyr Ser Arg Ser His Glu
275 280 285
Glu Trp Leu Lys Asn Met Asp Lys Asn Ile Val Glu Phe Lys Glu Ile
290 295 300
Met Arg Ser Ile Thr Lys Thr Glu Lys Glu Ala Ile Lys Leu Leu Asn
305 310 315 320
Phe Trp Arg Ile Phe Cys Met Cys Gly Ala Glu Leu Phe Gly Tyr Lys
325 330 335
Asn Gly Glu Glu Trp Met Leu Thr His Leu Leu Phe Lys Lys Lys
340 345 350
<210> 31
<211> 1563
<212> DNA
<213> Unkown
<400> 31
atgaaaccag aagatttccg cgccagtacc caacgtcctt tcaccgggga agagtatctg 60
aaaagcctgc aggatggtcg cgagatctat atctatggcg agcgagtgaa agacgtcacc 120
actcatccgg catttcgtaa tgcggcagcg tctgttgccc agctgtacga cgcactgcac 180
aaaccggaga tgcaggactc tctgtgttgg aacaccgaca ccggcagcgg cggctatacc 240
cataaattct tccgcgtggc gaaaagtgcc gacgacctgc gccagcaacg cgacgccatc 300
gctgagtggt cacgcctgag ctatggctgg atgggccgta ccccagacta caaagccgct 360
ttcggttgcg cactgggcgc gaatccgggc ttttacggtc agttcgagca gaacgcccgt 420
aactggtaca cccgtattca ggaaactggc ctctacttta accacgcgat tgttaaccca 480
ccgatcgatc gtcatttgcc gaccgataaa gtgaaagacg tttacatcaa gctggaaaaa 540
gagactgacg ccgggattat cgtcagcggt gcgaaagtgg ttgccaccaa ctcggcgctg 600
actcactaca acatgattgg cttcggctcg gcacaagtga tgggcgaaaa cccggacttc 660
gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga aattaatctc ccgcgcctct 720
tatgagatgg tcgcgggtgc taccggctcg ccatacgact acccgctctc cagccgcttc 780
gatgagaacg atgcgattct ggtgatggat aacgtgctga ttccatggga aaacgtgctg 840
atctaccgcg attttgatcg ctgccgtcgc tggacgatgg aaggcggttt tgcccgtatg 900
tatccgctgc aagcctgtgt gcgcctggca gtgaaattag acttcattac ggcactgctg 960
aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg gtgtgcaggc cgatctcggt 1020
gaagtggtag cgtggcgcaa caccttctgg gcattgagtg actcgatgtg ttcagaagca 1080
acgccgtggg tcaacggggc ttatttaccg gatcatgccg cactgcaaac ctatcgcgta 1140
ctggcaccaa tggcctacgc gaagatcaaa aacattatcg aacgcaacgt taccagtggc 1200
ctgatctatc tcccttccag tgcccgtgac ctgaataatc cgcagatcga ccagtatctg 1260
gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc agcgcatcaa gatcctcaaa 1320
ctgatgtggg atgctattgg cagcgaattt ggtggtcgtc acgaactgta tgaaatcaac 1380
tactccggta gccaggatga gattcgcctg cagtgtctgc gccaggcaca aaactccggc 1440
aatatggaca agatgatggc gatggttgat cgctgcctgt cggaatacga ccaggacggc 1500
tggactgtgc cgcacctgca caacaacgac gatatcaaca tgctggataa gctgctgaaa 1560
taa 1563
<210> 32
<211> 520
<212> PRT
<213> Unkown
<400> 32
Met Lys Pro Glu Asp Phe Arg Ala Ser Thr Gln Arg Pro Phe Thr Gly
1 5 10 15
Glu Glu Tyr Leu Lys Ser Leu Gln Asp Gly Arg Glu Ile Tyr Ile Tyr
20 25 30
Gly Glu Arg Val Lys Asp Val Thr Thr His Pro Ala Phe Arg Asn Ala
35 40 45
Ala Ala Ser Val Ala Gln Leu Tyr Asp Ala Leu His Lys Pro Glu Met
50 55 60
Gln Asp Ser Leu Cys Trp Asn Thr Asp Thr Gly Ser Gly Gly Tyr Thr
65 70 75 80
His Lys Phe Phe Arg Val Ala Lys Ser Ala Asp Asp Leu Arg Gln Gln
85 90 95
Arg Asp Ala Ile Ala Glu Trp Ser Arg Leu Ser Tyr Gly Trp Met Gly
100 105 110
Arg Thr Pro Asp Tyr Lys Ala Ala Phe Gly Cys Ala Leu Gly Ala Asn
115 120 125
Pro Gly Phe Tyr Gly Gln Phe Glu Gln Asn Ala Arg Asn Trp Tyr Thr
130 135 140
Arg Ile Gln Glu Thr Gly Leu Tyr Phe Asn His Ala Ile Val Asn Pro
145 150 155 160
Pro Ile Asp Arg His Leu Pro Thr Asp Lys Val Lys Asp Val Tyr Ile
165 170 175
Lys Leu Glu Lys Glu Thr Asp Ala Gly Ile Ile Val Ser Gly Ala Lys
180 185 190
Val Val Ala Thr Asn Ser Ala Leu Thr His Tyr Asn Met Ile Gly Phe
195 200 205
Gly Ser Ala Gln Val Met Gly Glu Asn Pro Asp Phe Ala Leu Met Phe
210 215 220
Val Ala Pro Met Asp Ala Asp Gly Val Lys Leu Ile Ser Arg Ala Ser
225 230 235 240
Tyr Glu Met Val Ala Gly Ala Thr Gly Ser Pro Tyr Asp Tyr Pro Leu
245 250 255
Ser Ser Arg Phe Asp Glu Asn Asp Ala Ile Leu Val Met Asp Asn Val
260 265 270
Leu Ile Pro Trp Glu Asn Val Leu Ile Tyr Arg Asp Phe Asp Arg Cys
275 280 285
Arg Arg Trp Thr Met Glu Gly Gly Phe Ala Arg Met Tyr Pro Leu Gln
290 295 300
Ala Cys Val Arg Leu Ala Val Lys Leu Asp Phe Ile Thr Ala Leu Leu
305 310 315 320
Lys Lys Ser Leu Glu Cys Thr Gly Thr Leu Glu Phe Arg Gly Val Gln
325 330 335
Ala Asp Leu Gly Glu Val Val Ala Trp Arg Asn Thr Phe Trp Ala Leu
340 345 350
Ser Asp Ser Met Cys Ser Glu Ala Thr Pro Trp Val Asn Gly Ala Tyr
355 360 365
Leu Pro Asp His Ala Ala Leu Gln Thr Tyr Arg Val Leu Ala Pro Met
370 375 380
Ala Tyr Ala Lys Ile Lys Asn Ile Ile Glu Arg Asn Val Thr Ser Gly
385 390 395 400
Leu Ile Tyr Leu Pro Ser Ser Ala Arg Asp Leu Asn Asn Pro Gln Ile
405 410 415
Asp Gln Tyr Leu Ala Lys Tyr Val Arg Gly Ser Asn Gly Met Asp His
420 425 430
Val Gln Arg Ile Lys Ile Leu Lys Leu Met Trp Asp Ala Ile Gly Ser
435 440 445
Glu Phe Gly Gly Arg His Glu Leu Tyr Glu Ile Asn Tyr Ser Gly Ser
450 455 460
Gln Asp Glu Ile Arg Leu Gln Cys Leu Arg Gln Ala Gln Asn Ser Gly
465 470 475 480
Asn Met Asp Lys Met Met Ala Met Val Asp Arg Cys Leu Ser Glu Tyr
485 490 495
Asp Gln Asp Gly Trp Thr Val Pro His Leu His Asn Asn Asp Asp Ile
500 505 510
Asn Met Leu Asp Lys Leu Leu Lys
515 520
<210> 33
<211> 513
<212> DNA
<213> Unkown
<400> 33
atgcaattag atgaacaacg cctgcgcttt cgtgacgcga tggccagcct gtcggcagcg 60
gtaaatatta tcaccaccga gggcgacgcc ggacaatgcg ggattacggc aacggccgtc 120
tgctcggtca cggatacacc accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg 180
aacccggttt ttcagggcaa cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa 240
ctgatggcac gccacttcgc gggcatgaca ggcatggcga tggaagagcg ttttagcctc 300
tcatgctggc aaaaaggtcc gctggcgcag ccggtgctaa aaggttcgct ggccagtctt 360
gaaggtgaga tccgcgatgt gcaggcaatt ggcacacatc tggtgtatct ggtggagatt 420
aaaaacatca tcctcagtgc agaaggtcat ggacttatct actttaaacg ccgtttccat 480
ccggtgatgc tggaaatgga agctgcgatt taa 513
<210> 34
<211> 170
<212> PRT
<213> Unkown
<400> 34
Met Gln Leu Asp Glu Gln Arg Leu Arg Phe Arg Asp Ala Met Ala Ser
1 5 10 15
Leu Ser Ala Ala Val Asn Ile Ile Thr Thr Glu Gly Asp Ala Gly Gln
20 25 30
Cys Gly Ile Thr Ala Thr Ala Val Cys Ser Val Thr Asp Thr Pro Pro
35 40 45
Ser Leu Met Val Cys Ile Asn Ala Asn Ser Ala Met Asn Pro Val Phe
50 55 60
Gln Gly Asn Gly Lys Leu Cys Val Asn Val Leu Asn His Glu Gln Glu
65 70 75 80
Leu Met Ala Arg His Phe Ala Gly Met Thr Gly Met Ala Met Glu Glu
85 90 95
Arg Phe Ser Leu Ser Cys Trp Gln Lys Gly Pro Leu Ala Gln Pro Val
100 105 110
Leu Lys Gly Ser Leu Ala Ser Leu Glu Gly Glu Ile Arg Asp Val Gln
115 120 125
Ala Ile Gly Thr His Leu Val Tyr Leu Val Glu Ile Lys Asn Ile Ile
130 135 140
Leu Ser Ala Glu Gly His Gly Leu Ile Tyr Phe Lys Arg Arg Phe His
145 150 155 160
Pro Val Met Leu Glu Met Glu Ala Ala Ile
165 170
<210> 35
<211> 3525
<212> DNA
<213> Unkown
<400> 35
atgagtccga tcacacgcga ggagcgcctg gagcgtcgta ttcaagacct gtacgccaat 60
gatccgcaat ttgccgcagc caagccggca acagcaatta ccgccgcaat cgaacgtccg 120
ggcctgcctc tgcctcagat tattgaaacc gttatgacag gctatgccga ccgtcctgca 180
ctggcccaac gtagtgtgga atttgtgaca gatgcaggca ccggtcacac caccttacgc 240
ctgctgccgc acttcgagac catcagttac ggcgaactgt gggatcgcat tagtgccctg 300
gccgatgttt taagcaccga gcagaccgtg aaaccgggtg atcgcgtttg cctgctgggt 360
ttcaacagcg tggattatgc aacaattgac atgaccctgg cccgcctggg tgcagtggcc 420
gtgccgctgc aaaccagcgc agcaattaca cagctgcagc ctattgtggc cgaaacccag 480
ccgaccatga ttgcagccag cgttgacgcc ttagcagatg ccacagagtt agcactgagt 540
ggtcagaccg caacacgcgt gctggttttc gaccatcatc gccaggttga tgcacatcgc 600
gccgcagttg aaagtgcacg cgaacgcctg gccggcagtg cagtggttga gaccttagca 660
gaagccattg cacgtggtga cgtgccgcgt ggtgcaagtg caggtagtgc cccgggcaca 720
gacgttagtg atgacagtct ggccctgctg atttacacca gtggcagtac aggcgccccg 780
aagggtgcaa tgtatcctcg tcgcaatgtt gccacattct ggcgcaaacg cacctggttt 840
gaaggtggtt acgaaccgag tatcaccctg aacttcatgc cgatgagcca tgtgatgggt 900
cgccagatcc tgtacggcac cctgtgcaat ggcggcaccg cctacttcgt tgcaaagagc 960
gatctgagca cactgtttga agacctggca ttagtgcgcc cgaccgaatt aaccttcgtg 1020
ccgcgtgttt gggacatggt tttcgatgaa tttcagagcg aagttgaccg ccgcctggtt 1080
gatggtgcag atcgtgttgc cctggaggcc caggttaaag ccgagatccg taatgacgtg 1140
ttaggcggcc gctatacaag cgcactgacc ggcagtgcac cgattagcga tgagatgaag 1200
gcctgggttg aagagctgct ggacatgcac ctggtggaag gttacggcag tacagaagcc 1260
ggcatgatcc tgattgatgg cgcaattcgt cgccctgcag ttctggacta caaactggtg 1320
gatgtgccgg acctgggtta cttcttaacc gaccgccctc atccgcgcgg tgaactgctg 1380
gtgaagaccg acagcttatt ccctggttat taccagcgcg ccgaggttac cgcagacgtt 1440
tttgacgccg atggtttcta tcgcaccggc gacattatgg ccgaagtggg ccctgaacag 1500
ttcgtgtacc tggatcgtcg taataacgtt ctgaaattaa gtcagggcga gtttgttaca 1560
gtgagcaaac tggaggcagt gttcggtgac agcccgctgg tgcgtcagat ctatatctat 1620
ggcaacagtg cccgcgcata cctgctggca gtgatcgtgc ctacccaaga ggccctggat 1680
gccgttcctg ttgaggaatt aaaagcccgc ctgggcgata gcctgcagga agtggccaaa 1740
gcagccggtc tgcaaagtta cgaaatcccg cgcgacttca ttatcgaaac caccccgtgg 1800
acactggaga acggcctgct gacaggtatc cgtaaactgg cccgcccgca gctgaagaag 1860
cactatggcg agctgctgga gcagatctat acagacctgg cccacggcca agccgatgag 1920
ctgcgtagcc tgcgccagag tggtgccgat gcacctgtgc tggtgaccgt ttgtcgcgca 1980
gcagccgcct tactgggtgg tagcgccagc gatgtgcagc ctgacgccca cttcacagac 2040
ctgggcggtg atagcttaag tgccctgagt tttaccaacc tgctgcacga aatcttcgat 2100
atcgaggtgc ctgttggtgt gattgttagc ccggccaacg atttacaggc actggccgat 2160
tatgttgagg cagcacgtaa acctggcagc agtcgcccga cattcgccag cgttcatggt 2220
gccagcaatg gccaagtgac agaggtgcat gccggcgacc tgagtctgga taaatttatc 2280
gacgccgcca ccctggccga agcacctcgt ttaccggcag ccaataccca ggttcgcaca 2340
gtgctgctga ccggcgccac aggttttctg ggccgttatc tggcattaga gtggctggag 2400
cgtatggatc tggttgacgg caagctgatc tgcctggttc gcgcaaagag tgacacagaa 2460
gcccgtgcac gtttagacaa gacattcgac agcggcgatc cggaactgtt agcccactat 2520
cgcgcattag caggcgatca tctggaagtg ctggcaggtg ataagggcga agccgatctg 2580
ggtctggacc gccagacctg gcaacgctta gcagacacag tggacctgat cgttgatcct 2640
gcagcactgg tgaaccacgt gctgccgtat agtcagctgt ttggcccgaa tgccctgggt 2700
accgcagaac tgctgcgcct ggccctgacc agtaaaatta agccgtatag ttacacaagc 2760
accattggcg tggccgacca aattcctccg agcgcattta ccgaggatgc cgatatccgc 2820
gttattagtg ccacacgtgc agttgacgac agttacgcaa atggttacag caacagcaag 2880
tgggccggcg aggttctgct gcgcgaagca catgacctgt gcggcctgcc ggttgccgtg 2940
tttcgttgcg atatgatcct ggccgatacc acctgggcag gtcagttaaa cgtgccggat 3000
atgttcaccc gcatgatttt aagcttagcc gccaccggta tcgccccggg tagcttttat 3060
gagctggcag cagatggcgc ccgtcaacgc gcccactatg atggcctgcc ggtggagttt 3120
atcgcagagg ccattagcac actgggtgca cagagccagg acggcttcca tacctatcac 3180
gtgatgaatc cgtatgatga cggcatcggt ctggacgagt tcgttgattg gctgaacgaa 3240
agcggctgtc ctatccagcg cattgccgac tacggcgact ggctgcaacg tttcgagacc 3300
gcactgcgtg ccctgcctga tcgtcagcgc cacagtagtc tgttaccttt actgcacaac 3360
taccgtcagc ctgaacgtcc ggttcgcggt agcattgcac cgaccgaccg ttttcgcgca 3420
gccgtgcaag aggcgaagat tggcccggat aaagatattc cgcacgtggg cgccccgatc 3480
atcgtgaaat atgtgagtga cctgcgcctg ttaggcctgc tgtaa 3525
<210> 36
<211> 1174
<212> PRT
<213> Unkown
<400> 36
Met Ser Pro Ile Thr Arg Glu Glu Arg Leu Glu Arg Arg Ile Gln Asp
1 5 10 15
Leu Tyr Ala Asn Asp Pro Gln Phe Ala Ala Ala Lys Pro Ala Thr Ala
20 25 30
Ile Thr Ala Ala Ile Glu Arg Pro Gly Leu Pro Leu Pro Gln Ile Ile
35 40 45
Glu Thr Val Met Thr Gly Tyr Ala Asp Arg Pro Ala Leu Ala Gln Arg
50 55 60
Ser Val Glu Phe Val Thr Asp Ala Gly Thr Gly His Thr Thr Leu Arg
65 70 75 80
Leu Leu Pro His Phe Glu Thr Ile Ser Tyr Gly Glu Leu Trp Asp Arg
85 90 95
Ile Ser Ala Leu Ala Asp Val Leu Ser Thr Glu Gln Thr Val Lys Pro
100 105 110
Gly Asp Arg Val Cys Leu Leu Gly Phe Asn Ser Val Asp Tyr Ala Thr
115 120 125
Ile Asp Met Thr Leu Ala Arg Leu Gly Ala Val Ala Val Pro Leu Gln
130 135 140
Thr Ser Ala Ala Ile Thr Gln Leu Gln Pro Ile Val Ala Glu Thr Gln
145 150 155 160
Pro Thr Met Ile Ala Ala Ser Val Asp Ala Leu Ala Asp Ala Thr Glu
165 170 175
Leu Ala Leu Ser Gly Gln Thr Ala Thr Arg Val Leu Val Phe Asp His
180 185 190
His Arg Gln Val Asp Ala His Arg Ala Ala Val Glu Ser Ala Arg Glu
195 200 205
Arg Leu Ala Gly Ser Ala Val Val Glu Thr Leu Ala Glu Ala Ile Ala
210 215 220
Arg Gly Asp Val Pro Arg Gly Ala Ser Ala Gly Ser Ala Pro Gly Thr
225 230 235 240
Asp Val Ser Asp Asp Ser Leu Ala Leu Leu Ile Tyr Thr Ser Gly Ser
245 250 255
Thr Gly Ala Pro Lys Gly Ala Met Tyr Pro Arg Arg Asn Val Ala Thr
260 265 270
Phe Trp Arg Lys Arg Thr Trp Phe Glu Gly Gly Tyr Glu Pro Ser Ile
275 280 285
Thr Leu Asn Phe Met Pro Met Ser His Val Met Gly Arg Gln Ile Leu
290 295 300
Tyr Gly Thr Leu Cys Asn Gly Gly Thr Ala Tyr Phe Val Ala Lys Ser
305 310 315 320
Asp Leu Ser Thr Leu Phe Glu Asp Leu Ala Leu Val Arg Pro Thr Glu
325 330 335
Leu Thr Phe Val Pro Arg Val Trp Asp Met Val Phe Asp Glu Phe Gln
340 345 350
Ser Glu Val Asp Arg Arg Leu Val Asp Gly Ala Asp Arg Val Ala Leu
355 360 365
Glu Ala Gln Val Lys Ala Glu Ile Arg Asn Asp Val Leu Gly Gly Arg
370 375 380
Tyr Thr Ser Ala Leu Thr Gly Ser Ala Pro Ile Ser Asp Glu Met Lys
385 390 395 400
Ala Trp Val Glu Glu Leu Leu Asp Met His Leu Val Glu Gly Tyr Gly
405 410 415
Ser Thr Glu Ala Gly Met Ile Leu Ile Asp Gly Ala Ile Arg Arg Pro
420 425 430
Ala Val Leu Asp Tyr Lys Leu Val Asp Val Pro Asp Leu Gly Tyr Phe
435 440 445
Leu Thr Asp Arg Pro His Pro Arg Gly Glu Leu Leu Val Lys Thr Asp
450 455 460
Ser Leu Phe Pro Gly Tyr Tyr Gln Arg Ala Glu Val Thr Ala Asp Val
465 470 475 480
Phe Asp Ala Asp Gly Phe Tyr Arg Thr Gly Asp Ile Met Ala Glu Val
485 490 495
Gly Pro Glu Gln Phe Val Tyr Leu Asp Arg Arg Asn Asn Val Leu Lys
500 505 510
Leu Ser Gln Gly Glu Phe Val Thr Val Ser Lys Leu Glu Ala Val Phe
515 520 525
Gly Asp Ser Pro Leu Val Arg Gln Ile Tyr Ile Tyr Gly Asn Ser Ala
530 535 540
Arg Ala Tyr Leu Leu Ala Val Ile Val Pro Thr Gln Glu Ala Leu Asp
545 550 555 560
Ala Val Pro Val Glu Glu Leu Lys Ala Arg Leu Gly Asp Ser Leu Gln
565 570 575
Glu Val Ala Lys Ala Ala Gly Leu Gln Ser Tyr Glu Ile Pro Arg Asp
580 585 590
Phe Ile Ile Glu Thr Thr Pro Trp Thr Leu Glu Asn Gly Leu Leu Thr
595 600 605
Gly Ile Arg Lys Leu Ala Arg Pro Gln Leu Lys Lys His Tyr Gly Glu
610 615 620
Leu Leu Glu Gln Ile Tyr Thr Asp Leu Ala His Gly Gln Ala Asp Glu
625 630 635 640
Leu Arg Ser Leu Arg Gln Ser Gly Ala Asp Ala Pro Val Leu Val Thr
645 650 655
Val Cys Arg Ala Ala Ala Ala Leu Leu Gly Gly Ser Ala Ser Asp Val
660 665 670
Gln Pro Asp Ala His Phe Thr Asp Leu Gly Gly Asp Ser Leu Ser Ala
675 680 685
Leu Ser Phe Thr Asn Leu Leu His Glu Ile Phe Asp Ile Glu Val Pro
690 695 700
Val Gly Val Ile Val Ser Pro Ala Asn Asp Leu Gln Ala Leu Ala Asp
705 710 715 720
Tyr Val Glu Ala Ala Arg Lys Pro Gly Ser Ser Arg Pro Thr Phe Ala
725 730 735
Ser Val His Gly Ala Ser Asn Gly Gln Val Thr Glu Val His Ala Gly
740 745 750
Asp Leu Ser Leu Asp Lys Phe Ile Asp Ala Ala Thr Leu Ala Glu Ala
755 760 765
Pro Arg Leu Pro Ala Ala Asn Thr Gln Val Arg Thr Val Leu Leu Thr
770 775 780
Gly Ala Thr Gly Phe Leu Gly Arg Tyr Leu Ala Leu Glu Trp Leu Glu
785 790 795 800
Arg Met Asp Leu Val Asp Gly Lys Leu Ile Cys Leu Val Arg Ala Lys
805 810 815
Ser Asp Thr Glu Ala Arg Ala Arg Leu Asp Lys Thr Phe Asp Ser Gly
820 825 830
Asp Pro Glu Leu Leu Ala His Tyr Arg Ala Leu Ala Gly Asp His Leu
835 840 845
Glu Val Leu Ala Gly Asp Lys Gly Glu Ala Asp Leu Gly Leu Asp Arg
850 855 860
Gln Thr Trp Gln Arg Leu Ala Asp Thr Val Asp Leu Ile Val Asp Pro
865 870 875 880
Ala Ala Leu Val Asn His Val Leu Pro Tyr Ser Gln Leu Phe Gly Pro
885 890 895
Asn Ala Leu Gly Thr Ala Glu Leu Leu Arg Leu Ala Leu Thr Ser Lys
900 905 910
Ile Lys Pro Tyr Ser Tyr Thr Ser Thr Ile Gly Val Ala Asp Gln Ile
915 920 925
Pro Pro Ser Ala Phe Thr Glu Asp Ala Asp Ile Arg Val Ile Ser Ala
930 935 940
Thr Arg Ala Val Asp Asp Ser Tyr Ala Asn Gly Tyr Ser Asn Ser Lys
945 950 955 960
Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp Leu Cys Gly Leu
965 970 975
Pro Val Ala Val Phe Arg Cys Asp Met Ile Leu Ala Asp Thr Thr Trp
980 985 990
Ala Gly Gln Leu Asn Val Pro Asp Met Phe Thr Arg Met Ile Leu Ser
995 1000 1005
Leu Ala Ala Thr Gly Ile Ala Pro Gly Ser Phe Tyr Glu Leu Ala Ala
1010 1015 1020
Asp Gly Ala Arg Gln Arg Ala His Tyr Asp Gly Leu Pro Val Glu Phe
1025 1030 1035 1040
Ile Ala Glu Ala Ile Ser Thr Leu Gly Ala Gln Ser Gln Asp Gly Phe
1045 1050 1055
His Thr Tyr His Val Met Asn Pro Tyr Asp Asp Gly Ile Gly Leu Asp
1060 1065 1070
Glu Phe Val Asp Trp Leu Asn Glu Ser Gly Cys Pro Ile Gln Arg Ile
1075 1080 1085
Ala Asp Tyr Gly Asp Trp Leu Gln Arg Phe Glu Thr Ala Leu Arg Ala
1090 1095 1100
Leu Pro Asp Arg Gln Arg His Ser Ser Leu Leu Pro Leu Leu His Asn
1105 1110 1115 1120
Tyr Arg Gln Pro Glu Arg Pro Val Arg Gly Ser Ile Ala Pro Thr Asp
1125 1130 1135
Arg Phe Arg Ala Ala Val Gln Glu Ala Lys Ile Gly Pro Asp Lys Asp
1140 1145 1150
Ile Pro His Val Gly Ala Pro Ile Ile Val Lys Tyr Val Ser Asp Leu
1155 1160 1165
Arg Leu Leu Gly Leu Leu
1170
<210> 37
<211> 675
<212> DNA
<213> Unkown
<400> 37
atgaagattt acggaattta tatggaccgc ccgctttcac aggaagaaaa tgaacggttc 60
atgactttca tatcacctga aaaacgggag aaatgccgga gattttatca taaagaagat 120
gctcaccgca ccctgctggg agatgtgctc gttcgctcag tcataagcag gcagtatcag 180
ttggacaaat ccgatatccg ctttagcacg caggaatacg ggaagccgtg catccctgat 240
cttcccgacg ctcatttcaa catttctcac tccggccgct gggtcattgg tgcgtttgat 300
tcacagccga tcggcataga tatcgaaaaa acgaaaccga tcagccttga gatcgccaag 360
cgcttctttt caaaaacaga gtacagcgac cttttagcaa aagacaagga cgagcagaca 420
gactattttt atcatctatg gtcaatgaaa gaaagcttta tcaaacagga aggcaaaggc 480
ttatcgcttc cgcttgattc cttttcagtg cgcctgcatc aggacggaca agtatccatt 540
gagcttccgg acagccattc cccatgctat atcaaaacgt atgaggtcga tcccggctac 600
aaaatggctg tatgcgccgc acaccctgat ttccccgagg atatcacaat ggtctcgtac 660
gaagagcttt tataa 675
<210> 38
<211> 224
<212> PRT
<213> Unkown
<400> 38
Met Lys Ile Tyr Gly Ile Tyr Met Asp Arg Pro Leu Ser Gln Glu Glu
1 5 10 15
Asn Glu Arg Phe Met Thr Phe Ile Ser Pro Glu Lys Arg Glu Lys Cys
20 25 30
Arg Arg Phe Tyr His Lys Glu Asp Ala His Arg Thr Leu Leu Gly Asp
35 40 45
Val Leu Val Arg Ser Val Ile Ser Arg Gln Tyr Gln Leu Asp Lys Ser
50 55 60
Asp Ile Arg Phe Ser Thr Gln Glu Tyr Gly Lys Pro Cys Ile Pro Asp
65 70 75 80
Leu Pro Asp Ala His Phe Asn Ile Ser His Ser Gly Arg Trp Val Ile
85 90 95
Gly Ala Phe Asp Ser Gln Pro Ile Gly Ile Asp Ile Glu Lys Thr Lys
100 105 110
Pro Ile Ser Leu Glu Ile Ala Lys Arg Phe Phe Ser Lys Thr Glu Tyr
115 120 125
Ser Asp Leu Leu Ala Lys Asp Lys Asp Glu Gln Thr Asp Tyr Phe Tyr
130 135 140
His Leu Trp Ser Met Lys Glu Ser Phe Ile Lys Gln Glu Gly Lys Gly
145 150 155 160
Leu Ser Leu Pro Leu Asp Ser Phe Ser Val Arg Leu His Gln Asp Gly
165 170 175
Gln Val Ser Ile Glu Leu Pro Asp Ser His Ser Pro Cys Tyr Ile Lys
180 185 190
Thr Tyr Glu Val Asp Pro Gly Tyr Lys Met Ala Val Cys Ala Ala His
195 200 205
Pro Asp Phe Pro Glu Asp Ile Thr Met Val Ser Tyr Glu Glu Leu Leu
210 215 220
<210> 39
<211> 1488
<212> DNA
<213> Unkown
<400> 39
atgaattttc atcatctggc ttactggcag gataaagcgt taagtctcgc cattgaaaac 60
cgcttattta ttaacggtga atatactgct gcggcggaaa atgaaacctt tgaaaccgtt 120
gatccggtca cccaggcacc gctggcgaaa attgcccgcg gcaagagcgt cgatatcgac 180
cgtgcgatga gcgcagcacg cggcgtattt gaacgcggcg actggtcact ctcttctccg 240
gctaaacgta aagcggtact gaataaactc gccgatttaa tggaagccca cgccgaagag 300
ctggcactgc tggaaactct cgacaccggc aaaccgattc gtcacagtct gcgtgatgat 360
attcccggcg cggcgcgcgc cattcgctgg tacgccgaag cgatcgacaa agtgtatggc 420
gaagtggcga ccaccagtag ccatgagctg gcgatgatcg tgcgtgaacc ggtcggcgtg 480
attgccgcca tcgtgccgtg gaacttcccg ctgttgctga cttgctggaa actcggcccg 540
gcgctggcgg cgggaaacag cgtgattcta aaaccgtctg aaaaatcacc gctcagtgcg 600
attcgtctcg cggggctggc gaaagaagca ggcttgccgg atggtgtgtt gaacgtggtg 660
acgggttttg gtcatgaagc cgggcaggcg ctgtcgcgtc ataacgatat cgacgccatt 720
gcctttaccg gttcaacccg taccgggaaa cagctgctga aagatgcggg cgacagcaac 780
atgaaacgcg tctggctgga agcgggcggc aaaagcgcca acatcgtttt cgctgactgc 840
ccggatttgc aacaggcggc aagcgccacc gcagcaggca ttttctacaa ccagggacag 900
gtgtgcatcg ccggaacgcg cctgttgctg gaagagagca tcgccgatga attcttagcc 960
ctgttaaaac agcaggcgca aaactggcag ccgggccatc cacttgatcc cgcaaccacc 1020
atgggcacct taatcgactg cgcccacgcc gactcggtcc atagctttat tcgggaaggc 1080
gaaagcaaag ggcaactgtt gttggatggc cgtaacgccg ggctggctgc cgccatcggc 1140
ccgaccatct ttgtggatgt ggacccgaat gcgtccttaa gtcgcgaaga gattttcggt 1200
ccggtgctgg tggtcacgcg tttcacatca gaagaacagg cgctacagct tgccaacgac 1260
agccagtacg gccttggcgc ggcggtatgg acgcgcgacc tctcccgcgc gcaccgcatg 1320
agccgacgcc tgaaagccgg ttccgtcttc gtcaataact acaacgacgg cgatatgacc 1380
gtgccgtttg gcggctataa gcagagcggc aacggtcgcg acaaatccct gcatgccctt 1440
gaaaaattca ctgaactgaa aaccatctgg ataagcctgg aggcctga 1488
<210> 40
<211> 495
<212> PRT
<213> Unkown
<400> 40
Met Asn Phe His His Leu Ala Tyr Trp Gln Asp Lys Ala Leu Ser Leu
1 5 10 15
Ala Ile Glu Asn Arg Leu Phe Ile Asn Gly Glu Tyr Thr Ala Ala Ala
20 25 30
Glu Asn Glu Thr Phe Glu Thr Val Asp Pro Val Thr Gln Ala Pro Leu
35 40 45
Ala Lys Ile Ala Arg Gly Lys Ser Val Asp Ile Asp Arg Ala Met Ser
50 55 60
Ala Ala Arg Gly Val Phe Glu Arg Gly Asp Trp Ser Leu Ser Ser Pro
65 70 75 80
Ala Lys Arg Lys Ala Val Leu Asn Lys Leu Ala Asp Leu Met Glu Ala
85 90 95
His Ala Glu Glu Leu Ala Leu Leu Glu Thr Leu Asp Thr Gly Lys Pro
100 105 110
Ile Arg His Ser Leu Arg Asp Asp Ile Pro Gly Ala Ala Arg Ala Ile
115 120 125
Arg Trp Tyr Ala Glu Ala Ile Asp Lys Val Tyr Gly Glu Val Ala Thr
130 135 140
Thr Ser Ser His Glu Leu Ala Met Ile Val Arg Glu Pro Val Gly Val
145 150 155 160
Ile Ala Ala Ile Val Pro Trp Asn Phe Pro Leu Leu Leu Thr Cys Trp
165 170 175
Lys Leu Gly Pro Ala Leu Ala Ala Gly Asn Ser Val Ile Leu Lys Pro
180 185 190
Ser Glu Lys Ser Pro Leu Ser Ala Ile Arg Leu Ala Gly Leu Ala Lys
195 200 205
Glu Ala Gly Leu Pro Asp Gly Val Leu Asn Val Val Thr Gly Phe Gly
210 215 220
His Glu Ala Gly Gln Ala Leu Ser Arg His Asn Asp Ile Asp Ala Ile
225 230 235 240
Ala Phe Thr Gly Ser Thr Arg Thr Gly Lys Gln Leu Leu Lys Asp Ala
245 250 255
Gly Asp Ser Asn Met Lys Arg Val Trp Leu Glu Ala Gly Gly Lys Ser
260 265 270
Ala Asn Ile Val Phe Ala Asp Cys Pro Asp Leu Gln Gln Ala Ala Ser
275 280 285
Ala Thr Ala Ala Gly Ile Phe Tyr Asn Gln Gly Gln Val Cys Ile Ala
290 295 300
Gly Thr Arg Leu Leu Leu Glu Glu Ser Ile Ala Asp Glu Phe Leu Ala
305 310 315 320
Leu Leu Lys Gln Gln Ala Gln Asn Trp Gln Pro Gly His Pro Leu Asp
325 330 335
Pro Ala Thr Thr Met Gly Thr Leu Ile Asp Cys Ala His Ala Asp Ser
340 345 350
Val His Ser Phe Ile Arg Glu Gly Glu Ser Lys Gly Gln Leu Leu Leu
355 360 365
Asp Gly Arg Asn Ala Gly Leu Ala Ala Ala Ile Gly Pro Thr Ile Phe
370 375 380
Val Asp Val Asp Pro Asn Ala Ser Leu Ser Arg Glu Glu Ile Phe Gly
385 390 395 400
Pro Val Leu Val Val Thr Arg Phe Thr Ser Glu Glu Gln Ala Leu Gln
405 410 415
Leu Ala Asn Asp Ser Gln Tyr Gly Leu Gly Ala Ala Val Trp Thr Arg
420 425 430
Asp Leu Ser Arg Ala His Arg Met Ser Arg Arg Leu Lys Ala Gly Ser
435 440 445
Val Phe Val Asn Asn Tyr Asn Asp Gly Asp Met Thr Val Pro Phe Gly
450 455 460
Gly Tyr Lys Gln Ser Gly Asn Gly Arg Asp Lys Ser Leu His Ala Leu
465 470 475 480
Glu Lys Phe Thr Glu Leu Lys Thr Ile Trp Ile Ser Leu Glu Ala
485 490 495
<210> 41
<211> 60
<212> DNA
<213> Unkown
<400> 41
atggctatcc ctgcatttgg tttaggtact ttccgtctga gtgtaggctg gagctgcttc 60
<210> 42
<211> 62
<212> DNA
<213> Unkown
<400> 42
ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggcatatga atatcctcct 60
ta 62
<210> 43
<211> 62
<212> DNA
<213> Unkown
<400> 43
atgcaacaaa aaatgattca atttagtggc gatgtctcac tggtgtaggc tggagctgct 60
tc 62
<210> 44
<211> 62
<212> DNA
<213> Unkown
<400> 44
tcacaccata tccagcgcag tttttccttt tggtgccgga tatcatatga atatcctcct 60
ta 62
<210> 45
<211> 64
<212> DNA
<213> Unkown
<400> 45
atgctacaaa attgcgcaca atcaaattgc cgcattattc ctaagtgtag gctggagctg 60
cttc 64
<210> 46
<211> 61
<212> DNA
<213> Unkown
<400> 46
ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc cccatatgaa tatcctcctt 60
a 61
<210> 47
<211> 61
<212> DNA
<213> Unkown
<400> 47
atgaacaact ttaatctgca caccccaacc cgcattctgt tgtgtaggct ggagctgctt 60
c 61
<210> 48
<211> 60
<212> DNA
<213> Unkown
<400> 48
ttagcgggcg gcttcgtata tacggcggct gacatccaac gcatatgaat atcctcctta 60
<210> 49
<211> 62
<212> DNA
<213> Unkown
<400> 49
atggctaatc caaccgttat taagctacag gatggcaatg tcgtgtaggc tggagctgct 60
tc 62
<210> 50
<211> 60
<212> DNA
<213> Unkown
<400> 50
ttagccgccg aactggtcag gatcgggacc gagacgcttg ccatatgaat atcctcctta 60
<210> 51
<211> 61
<212> DNA
<213> Unkown
<400> 51
atgaagatca aagctgttgg tgcatattcc gctaaacaac cgtgtaggct ggagctgctt 60
c 61
<210> 52
<211> 60
<212> DNA
<213> Unkown
<400> 52
tcagtctgtt agtgtgcgat tatcgataac aaaacgatat tcatatgaat atcctcctta 60
<210> 53
<211> 62
<212> DNA
<213> Unkown
<400> 53
atgtcgatga taaaaagcta tgccgcaaaa gaagcgggcg gcgtgtaggc tggagctgct 60
tc 62
<210> 54
<211> 63
<212> DNA
<213> Unkown
<400> 54
tcaaaaatcg gctttcaaca ccacgcggta acgcgcctta ccgtcatatg aatatcctcc 60
tta 63
<210> 55
<211> 49
<212> DNA
<213> Unkown
<400> 55
gtatctagaa agaggagata taatgaatta tcagaacgac gatttacgc 49
<210> 56
<211> 35
<212> DNA
<213> Unkown
<400> 56
tatggatcca ctagtttacc cgcgacgcgc tttta 35
<210> 57
<211> 44
<212> DNA
<213> Unkown
<400> 57
gtatctagaa agaggagata taatggttgc tgaattgacc gcat 44
<210> 58
<211> 38
<212> DNA
<213> Unkown
<400> 58
atatgtcgac actagtttac tggcgattgt cattcgcc 38
<210> 59
<211> 53
<212> DNA
<213> Unkown
<400> 59
atctctagat ttaagaagga gatataatgg cacctgttac aattgaaaag ttc 53
<210> 60
<211> 44
<212> DNA
<213> Unkown
<400> 60
tctggatccg ctagcctatt ttttatttct tttaagtgcc gctg 44
<210> 61
<211> 46
<212> DNA
<213> Unkown
<400> 61
tagtctagat ttaagaagga gatataatga cccccgaaca gttccg 46
<210> 62
<211> 38
<212> DNA
<213> Unkown
<400> 62
acaggatcca ctagtttagc tgccgccttt aatcacgt 38
<210> 63
<211> 54
<212> DNA
<213> Unkown
<400> 63
tactctagat ttaagaagga gatataatgc gtaaagtgat caaatatgat atgg 54
<210> 64
<211> 38
<212> DNA
<213> Unkown
<400> 64
tcaggatcca ctagtttagc tgcccagtaa acgaggaa 38
<210> 65
<211> 51
<212> DNA
<213> Unkown
<400> 65
ttgtctagat ttaagaagga gatataatgg agaccgttag caaaattgac c 51
<210> 66
<211> 42
<212> DNA
<213> Unkown
<400> 66
gcaggatcca ctagtttagt acggatatgc ttcaatcacg ct 42
<210> 67
<211> 47
<212> DNA
<213> Unkown
<400> 67
tactctagat ttaagaagga gatataatgg ccttccatgg caaggac 47
<210> 68
<211> 41
<212> DNA
<213> Unkown
<400> 68
tcaggatcca ctagtttaag gaaacacctc aatcacgctc t 41
<210> 69
<211> 53
<212> DNA
<213> Unkown
<400> 69
atctctagat ttaagaagga gatataatgc agttaaaaga aaaagaggag ctg 53
<210> 70
<211> 46
<212> DNA
<213> Unkown
<400> 70
agaggatcca ctagtttatt ttttcttaaa cagcagatgg gttaac 46
<210> 71
<211> 48
<212> DNA
<213> Unkown
<400> 71
tagtctagat ttaagaagga gatataatga aaccagaaga tttccgcg 48
<210> 72
<211> 33
<212> DNA
<213> Unkown
<400> 72
aacgctcgag ttaaatcgca gcttccattt cca 33
<210> 73
<211> 47
<212> DNA
<213> Unkown
<400> 73
atctctagat ttaagaagga gatataatga gtccgatcac acgcgag 47
<210> 74
<211> 36
<212> DNA
<213> Unkown
<400> 74
agaggatcca ctagtttaca gcaggcctaa caggcg 36
<210> 75
<211> 55
<212> DNA
<213> Unkown
<400> 75
gtctctagat ttaagaagga gatataatga agatttacgg aatttatatg gaccg 55
<210> 76
<211> 45
<212> DNA
<213> Unkown
<400> 76
tgaggatcca ctagtttata aaagctcttc gtacgagacc attgt 45
<210> 77
<211> 52
<212> DNA
<213> Unkown
<400> 77
atctctagat ttaagaagga gatataatga attttcatca tctggcttac tg 52
<210> 78
<211> 34
<212> DNA
<213> Unkown
<400> 78
tcaggatccg ctagctcagg cctccaggct atcc 34
Claims (5)
1.一种微生物,其特征在于所述微生物不含有或者敲除dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB基因,高表达或者导入aroGfbr、tyrAfb、KDC、HpaB、HpaC、DODC、CAR、sfp、aldH、CNS、4OMT、6OMT和CNMT基因;所述微生物为细菌、真菌、放线菌、螺旋体、支原体、衣原体或立克次体。
2.根据权利要求1所述的微生物,其特征在于:
所述dkgB基因具有SEQ ID NO:1所示的核苷酸序列或者所述dkgB基因编码SEQ ID NO:2所示的氨基酸序列;
所述yeaE基因具有SEQ ID NO:3所示的核苷酸序列或者所述yeaE基因编码SEQ ID NO:4所示的氨基酸序列;
所述yqhC基因具有SEQ ID NO:5所示的核苷酸序列或者所述yqhC基因编码SEQ ID NO:6所示的氨基酸序列;
所述yqhD基因具有SEQ ID NO:7所示的核苷酸序列或者所述yqhD基因编码SEQ ID NO:8所示的氨基酸序列;
所述dkgA基因具有SEQ ID NO:9所示的核苷酸序列或者所述dkgA基因编码SEQ ID NO:10所示的氨基酸序列;
所述yahK基因具有SEQ ID NO:11所示的核苷酸序列或者所述yahK基因编码SEQ IDNO:12所示的氨基酸序列;
所述yjgB基因具有SEQ ID NO:13所示的核苷酸序列或者所述yjgB基因编码SEQ IDNO:14所示的氨基酸序列;
所述aroGfbr基因具有SEQ ID NO:15所示的核苷酸序列或者所述aroGfbr基因编码SEQID NO:16所示的氨基酸序列;
所述tyrAfb基因具有SEQ ID NO:17所示的核苷酸序列或者所述tyrAfb基因编码SEQ IDNO:18所示的氨基酸序列;
所述KDC基因具有SEQ ID NO:19所示的核苷酸序列或者所述KDC基因编码SEQ ID NO:20所示的氨基酸序列;
所述DODC基因具有SEQ ID NO:21所示的核苷酸序列或者所述DODC基因编码SEQ IDNO:22所示的氨基酸序列;
所述CNS基因具有SEQ ID NO:23所示的核苷酸序列或者所述CNS基因编码SEQ ID NO:24所示的氨基酸序列;
所述6OMT基因具有SEQ ID NO:25所示的核苷酸序列或者所述6OMT基因编码SEQ IDNO:26所示的氨基酸序列;
所述4OMT基因具有SEQ ID NO:27所示的核苷酸序列或者所述4OMT基因编码SEQ IDNO:28所示的氨基酸序列;
所述CNMT基因具有SEQ ID NO:29所示的核苷酸序列或者所述NMCH基因编码SEQ IDNO:30所示的氨基酸序列;
所述HpaB基因具有SEQ ID NO:31所示的核苷酸序列或者所述HpaB基因编码SEQ IDNO:32所示的氨基酸序列;
所述HpaC基因具有SEQ ID NO:33所示的核苷酸序列或者所述HpaC基因编码SEQ IDNO:34所示的氨基酸序列;
所述CAR基因具有SEQ ID NO:35所示的核苷酸序列或者所述CAR基因编码SEQ ID NO:36所示的氨基酸序列;
所述sfp基因具有SEQ ID NO:37所示的核苷酸序列或者所述sfp基因编码SEQ ID NO:38所示的氨基酸序列;
所述aldH基因具有SEQ ID NO:39所示的核苷酸序列或者所述aldH基因编码SEQ IDNO:40所示的氨基酸序列。
3.根据权利要求1所述的微生物,其特征在于所述微生物为酵母或大肠杆菌。
4.权利要求1~3任意一项所述的微生物在生物合成牛心果碱中的应用。
5.一种微生物发酵葡萄糖合成牛心果碱的方法,其特征在于使用权利要求1~3中任意一项所述的微生物。
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