CN111220767A - Elastic buffer seat for testing biochip, testing module and testing equipment thereof - Google Patents
Elastic buffer seat for testing biochip, testing module and testing equipment thereof Download PDFInfo
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- CN111220767A CN111220767A CN201811413452.7A CN201811413452A CN111220767A CN 111220767 A CN111220767 A CN 111220767A CN 201811413452 A CN201811413452 A CN 201811413452A CN 111220767 A CN111220767 A CN 111220767A
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Abstract
The invention relates to an elastic buffer seat for testing a biological chip, a testing module and testing equipment thereof. Wherein, the buffer seat body is provided with a groove part and two through holes, and the two through holes comprise an input hole and an output hole; the extended outer edge is arranged on the periphery of the buffer seat body, the buffer seat body is concavely provided with a groove part with an arc-shaped periphery, and the two through holes are respectively positioned at two corresponding ends of the periphery of the groove part. Therefore, the test liquid can smoothly flow to the sensing area of the biochip, and turbulence caused by corner effect is avoided, so that a plurality of bubbles are generated; and after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and air bubbles can be prevented from remaining between the elastic buffer seat and the surface of the biochip.
Description
Background
The present invention relates to an elastic buffer seat for testing a biochip, a testing module thereof and a testing device, and more particularly, to an elastic buffer seat for testing a biochip under a testing liquid environment, a testing module thereof and a testing device.
The biochip of the present invention is a chip structure carrying a biosensor (Bio-Sensor), which is a device for detecting or specifically interacting with environmental chemicals in or out of a living body using a binding transducer for immobilizing biomolecules to generate a response. Among them, the application of biosensors is quite extensive, for example: the principle of ion sensing field effect can be used for detecting urea, or biological detection can be carried out on allergens, enzymes, microorganisms, cells and the like, or monitoring and analyzing environmental factors such as soil, ocean pH value and the like.
The biosensor of the present invention focuses on a semiconductor Ion sensor using an Ion Selective Field Effect Transistor (ISFET), which removes gate metal by applying the principle of MOSFET, and changes an insulating sensing film (SiO2) to directly contact with a test liquid, and provides a reference voltage by using an electrode, so that a test module can accurately measure a sensing signal of a biochip. Therefore, the biosensor must be tested in an environment in which the biosensor is in contact with the test liquid, and thus there are various design methods for loading the test liquid in the past documents, such as U.S. patent publication No. US2014/0239986, in which a water blocking portion (Fluid Well) is directly provided inside a probe Card (probe Card) so that a closed channel is formed when the biosensor is in contact with the probe Card, thereby inputting the test liquid into a test area of the biosensor.
Referring to fig. 1, a prior art elastic buffer base for biochip test, an elastic buffer base 9 includes: a buffer seat body 91 and an extended outer edge 92, the buffer seat body 91 has a groove portion 96 and two through holes 94, the two through holes 94 include an input hole and an output hole for inputting and outputting the testing liquid; the extended outer edge 92 is disposed on the periphery of the groove 96, the buffer base body forms a groove 96 with a rectangular periphery 97, and the two through holes 94 are disposed on two sides of the groove 96.
The peripheral edge of the groove portion 96 of the elastic buffer seat 9 is rectangular 97, the test liquid inputted from the input hole before the test is easy to cause turbulence due to the Corner Effect, and further generate many bubbles, which affects the continuous test result, and after the test is completed, the test liquid is sucked out from the output hole, and the test liquid cannot be completely sucked out due to the Corner Effect (Corner Effect) and the phenomenon of the surface tension of the test liquid, which causes part of the test liquid and bubbles to remain between the elastic buffer seat 9 and the surface of the biochip, and further pollutes the continuous test flow, so the elastic buffer seat 9 in the prior art still has a great improvement space in use.
The present inventors have therefore found that in the spirit of the present invention, we need to find an elastic buffer seat, a test module and a test apparatus for testing a biochip, which can solve the above problems.
Disclosure of Invention
The present invention provides an elastic buffer seat for biochip test, which has a groove structure with a specific geometry, so that test liquid can smoothly flow to a sensing region of a biochip before testing, thereby preventing turbulence caused by corner effect and further generating many bubbles to affect the subsequent testing result. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and bubbles are prevented from remaining between the elastic buffer seat and the surface of the biochip to further pollute the subsequent test flow.
In order to achieve the above object, the elastic buffer base for testing a biochip of the present invention includes a buffer base body and an extended outer edge. Wherein, the buffer seat body is provided with a groove part and two through holes, and the two through holes comprise an input hole and an output hole; the extended outer edge is arranged on the periphery of the buffer seat body, the buffer seat body is concavely provided with a groove part with an arc-shaped periphery, and the two through holes are respectively positioned at two corresponding ends of the periphery of the groove part.
The groove part can be elliptic, and the two through holes can be respectively positioned at the short arc part of the groove part. In addition, the groove part can also be in a circular shape, and the two through holes can be arranged at an interval of 180 degrees. Therefore, the two through holes are positioned at the periphery of the circular arc groove part, so that the test liquid can be smoothly extracted, and the test liquid and the bubbles are prevented from remaining between the elastic buffer seat and the surface of the biochip.
Each through hole can penetrate through the groove part and form a flange respectively so as to form a design for extending the output hole and the input hole, thereby being convenient for extracting the test liquid.
The groove of the buffer seat body has a bottom surface with a horizontal, oblique, arc-shaped convex part, arc-shaped concave part or other equivalent shapes, which can achieve the effect of smoothly pumping the test liquid.
The elastic buffer seat can be an integrated structure made of Polydimethylsiloxane (PDMS) or other equivalent materials, and the side wall of the groove part of the buffer seat body can be provided with a fillet.
The secondary objective of the present invention is to provide a testing module for biochip, which can reduce the pressing force when the pressure probe contacts with the biochip by the pressure probe with elastic buffer seat, and can effectively reduce the volume of the testing module by arranging the testing pipeline in the hollow casing, and provide a suitable environment to test the biochip under the contact with the testing liquid. In addition, the elastic buffer seat of the pressure measuring head passes through the groove part structure with a specific geometric shape, before the test, the test liquid can smoothly flow to the sensing area of the biochip, thereby avoiding turbulence caused by corner effect, further generating a plurality of bubbles and influencing the continuous test result. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and bubbles are prevented from remaining between the elastic buffer seat and the surface of the biochip to further pollute the subsequent test flow.
In order to achieve the above object, the testing module of biochip of the present invention includes a fixing base, a hollow casing and a pressure probe. The fixed seat is provided with a through hole, and the bottom of the fixed seat is fixedly provided with a probe card for performing functional test on the chip; the hollow sleeve penetrates through the through hole and is arranged in the fixing seat in a penetrating mode, at least two pipelines penetrate through the hollow sleeve, and a vertical and longitudinal accommodating space is provided and used for accommodating the pipelines. The pressure measuring head comprises a pressure measuring head body and an elastic buffer seat, the pressure measuring head body is arranged on the hollow sleeve, and the elastic buffer seat is clamped in the pressure measuring head body and connected with at least two pipelines. The elastic buffer seat comprises a buffer seat body and an extended outer edge, the buffer seat body is provided with a groove part and two through holes, the two through holes comprise an input hole and an output hole, the extended outer edge is arranged on the periphery of the groove part, the buffer seat body forms an arc-shaped groove part, and the two through holes are respectively positioned at the two corresponding ends of the periphery of the groove part.
The buffer seat body can be further provided with an electrode hole, an electrode penetrates through the hollow sleeve, and the electrode penetrates through the electrode hole. Therefore, the electrode can provide a reference voltage so that the test module can accurately measure the sensing signal of the biochip.
Another objective of the present invention is to provide a testing apparatus for biochip, which can accurately press and contact the probe and the biochip during the testing process, so that the sensing region on the biochip can be completely covered in the elastic buffer seat, and the fluidity of the testing solution in the elastic buffer seat is maintained, and the testing operation is performed in the environment containing the solution. In addition, the elastic buffer seat of the pressure measuring head passes through the groove part structure with a specific geometric shape, before the test, the test liquid can smoothly flow to the sensing area of the biochip, thereby avoiding turbulence caused by corner effect, further generating a plurality of bubbles and influencing the continuous test result. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and bubbles are prevented from remaining between the elastic buffer seat and the surface of the biochip to further pollute the subsequent test flow.
To achieve the above object, the testing apparatus for biochip of the present invention comprises: a probe card, a test module of a biochip and a test head. The probe card has a movable carrier for carrying a biochip. The testing module of the biological chip is placed on the movable carrying platform and comprises a fixed seat, a hollow sleeve and a pressure measuring head, wherein the fixed seat is provided with a through hole, and the bottom of the fixed seat is fixedly provided with a probe card; the hollow sleeve penetrates through the through hole and is arranged in the fixed seat in a penetrating way, wherein at least one pipeline is arranged in the hollow sleeve in a penetrating way; the pressure measuring head comprises a pressure measuring head body and an elastic buffer seat, the pressure measuring head body is arranged on the hollow sleeve, and the elastic buffer seat is clamped in the pressure measuring head body and connected with at least one pipeline. In addition, the test head is electrically connected with the probe card, wherein the test head is provided with a plurality of test circuit boards. The elastic buffer seat comprises a buffer seat body and an extended outer edge, the buffer seat body is provided with a groove part and two through holes, the two through holes comprise an input hole and an output hole, the extended outer edge is arranged on the periphery of the groove part, the buffer seat body forms an arc-shaped groove part, and the two through holes are respectively positioned at the two corresponding ends of the periphery of the groove part.
Both the foregoing general description and the following detailed description are exemplary and explanatory in nature to further illustrate the scope of the invention as claimed. Other objects and advantages of the present invention will become apparent from the following description and drawings.
Drawings
FIG. 1 is a perspective view of a prior art elastic buffer base for biochip test.
FIG. 2 is a perspective view of an elastic buffer base for biochip test according to a first preferred embodiment of the present invention.
Fig. 3 is a cross-sectional view of fig. 2.
Fig. 4 is another cross-sectional view of fig. 2.
FIG. 5 is a perspective view of an elastic buffer base for biochip test according to a second preferred embodiment of the present invention.
FIG. 6 is a perspective view of an elastic buffer base for biochip test according to a third preferred embodiment of the present invention.
FIG. 7 is a perspective view of an elastic buffer base for biochip test according to a fourth preferred embodiment of the present invention.
FIG. 8 is a perspective view of an elastic buffer base for biochip test according to a fifth preferred embodiment of the present invention.
FIG. 9 is a perspective view of an elastic buffer base for biochip test according to a sixth preferred embodiment of the present invention.
FIG. 10 is an exploded view of a test module of a biochip according to a preferred embodiment of the present invention.
FIG. 11 is a perspective cross-sectional view of a test module of a biochip according to a preferred embodiment of the present invention.
FIG. 12 is a perspective view of another perspective of a test module for biochips according to a preferred embodiment of the present invention.
FIG. 13 is a perspective view of a biochip measuring apparatus according to a preferred embodiment of the present invention.
FIG. 14 is a sectional view of a biochip measuring apparatus according to a preferred embodiment of the present invention
[ reference numerals ]
1 test module 10 test equipment
11 test head 12 biochip
13-pin measuring machine 131 moving carrying platform
14 test circuit board 15 signal line
2 holder 20 Probe card
21 through hole 22 fixing seat body
23 stop seat 231 stops inner edge
3 hollow sleeve 30 spring
31 backstop part 4 pressure measuring head
41 pressure gauge head body 42 elastic buffer seat
421 expanded outer edge 422 groove part
51 liquid inlet pipe 52 liquid outlet pipe
53 electrode
6, 60, 7, 70, 8, 9 elastic buffer seat 61, 601, 71, 701, 81, 91 buffer seat body
62, 602, 72, 702, 82, 92 extended outer edge 64, 604, 74, 704, 84, 94 through hole
66, 606, 76, 706, 86, 96, groove portions 662, 6062 peripheral edge
68, 608 liquid inlet 69, 609 liquid outlet
6063, 663 top surface 60611 arc-shaped concave part
6611 arcuately convex 705 flange
6061, 661, 73, 731 bottom surface 77 long arc
78 short arc 87 round
97 rectangular R fillet
Detailed Description
Please refer to fig. 2, fig. 3 and fig. 4, which are a perspective view of an elastic buffer base for biochip test according to a first preferred embodiment of the present invention and two cross-sectional views of fig. 2. The elastic buffer base 7 for biochip test of this embodiment includes: a buffer base body 71 and an extended outer edge 72, the buffer base body 71 having a bottom surface 73 and two through holes 74, the two through holes 74 including an input hole and an output hole for inputting and outputting the testing liquid; the extended outer edge 72 covers the bottom surface 73 to form a recessed portion 76 with an arc-shaped periphery, and the two through holes 74 are respectively located at two corresponding ends of the periphery of the recessed portion 76.
In the embodiment, the recessed portion 76 is oval, i.e. an oval composed of two long arcs 77 and two short arcs 78, and the two through holes 74 are respectively located at the periphery of the two short arcs 78 of the recessed portion 76. As shown in fig. 4, the bottom surface 73 of the cushion seat body 71 is horizontal. In the embodiment, the elastic buffer seat 7 is an integrated structure made of Polydimethylsiloxane (PDMS), and a rounded corner R (0.5mmx 45) is formed on the sidewall between the groove portion 76 and the bottom surface 73, so that the test liquid can be smoothly guided to the sensing region of the biochip at the rounded corner R, thereby avoiding turbulence caused by the corner effect.
Therefore, the elastic buffer seat 7 of the present embodiment can smoothly flow the testing liquid to the sensing region of the biochip before testing the biochip through the structure of the oval-shaped groove portion 76, thereby avoiding turbulence caused by corner effect and further generating many bubbles to affect the subsequent testing result. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and bubbles are prevented from remaining between the elastic buffer seat 7 and the surface of the biochip to further pollute the subsequent test flow.
When the elastic buffer seat 7 of the present embodiment is used for testing a biochip, the groove 76 of the elastic buffer seat 7 is used as a liquid accommodating groove, the groove 76 is a small groove with a depth of 1mm to 3mm, and an opening of the small groove needs to cover a sensing area on the biochip, so as to create a testing environment capable of storing testing liquid. In addition, the extension outer edge 72 of the embodiment is an extension outer edge extending to the periphery outside the outer wall at the bottom of the elastic buffer seat 7, and the extension outer edge 72 lengthens the leakage distance of the test liquid flowing out, so that the problem that the test liquid extrudes and leaks to an external circuit board or an external electrode when the elastic buffer seat 7 deforms under the stress can be avoided.
In addition, the material used for the elastic buffer seat 7 of the present embodiment is made of an elastic material such as Polydimethylsiloxane (PDMS) by molding. Wherein, the dimethyl siloxane (PDMS) is a transparent silica gel material, which has the characteristics of non-toxicity, high hydrophobicity, acid and alkali resistance, good structure elasticity and air tightness, etc., and the fabrication is simple and fast, the mold-turning precision can reach um-1, and the PDMS is used as a buffer and water-retaining structure in the embodiment. In addition, the elastic buffer seat 7 is easy to manufacture and low in cost, so that if the elastic buffer seat is damaged or deformed in the process of testing and pressing the biochip, the elastic buffer seat can be replaced, and the durability of the embodiment is improved through a replaceable consumable design mode.
Referring to fig. 5, a perspective view of an elastic buffer base for biochip test according to a second preferred embodiment of the present invention, the elastic buffer base for biochip test 70 of the second embodiment has substantially the same structure as the first embodiment, and only differs in that the buffer base body 71 of the second embodiment has an inclined bottom surface 731, wherein the inclined bottom surface 731 extends to an output hole to enable the test liquid in the groove portion 76 to be smoothly guided to the output hole for discharging, so that the groove portion 76 does not generate a bubble phenomenon.
Therefore, the elastic buffer seat 7 of the present embodiment can match the inclined bottom surface 731 through the elliptical groove portion 76 structure, before testing the biochip, the testing liquid can smoothly flow to the sensing region of the biochip, thereby avoiding turbulence caused by corner effect, and further generating many bubbles to affect the subsequent testing result. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and bubbles are prevented from remaining between the elastic buffer seat 7 and the surface of the biochip to further pollute the subsequent test flow.
Referring to fig. 6, a perspective view of an elastic buffer base for biochip test according to a third preferred embodiment of the present invention, the elastic buffer base 70 for biochip test of the third embodiment has substantially the same structure as the first embodiment, and the difference is only that two flanges 705 are added to the elastic buffer base 70 of the third embodiment, that is, the elastic buffer base 70 includes: a buffer seat body 701 and an extended outer edge 702, the buffer seat body 701 has a groove portion 706 and two through holes 704, the two through holes 704 include an input hole and an output hole for inputting and outputting the testing liquid; the extended rim 702 is disposed at the periphery of the buffer base body 701, and the buffer base body 701 forms an oval groove 706, and the two through holes 704 are respectively located at two opposite ends of the periphery of the oval groove 706. In addition, each through hole 704 is disposed through the recessed portion 706 and forms a flange 705 to form a design extending the output hole and the input hole, so as to facilitate the extraction of the testing liquid.
Therefore, the third embodiment is also the same as the first embodiment, and the elliptical groove 706 structure allows the testing liquid to smoothly flow to the sensing region of the biochip before testing the biochip, thereby avoiding turbulence caused by corner effect and further generating many bubbles to affect the subsequent testing results. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and air bubbles are prevented from remaining between the elastic buffer seat 70 and the surface of the biochip to further pollute the subsequent test flow.
Referring to fig. 7, a perspective view of the elastic buffer base for biochip test according to the fourth preferred embodiment of the present invention, the elastic buffer base 8 for biochip test of the fourth embodiment has substantially the same structure as the first embodiment, except that the groove portion 86 of the elastic buffer base 8 of the fourth embodiment is circular 87 instead of elliptical shape of the first embodiment, that is, the elastic buffer base 8 includes: a buffer seat body 81 and an extended outer edge 82, the buffer seat body 81 has a groove portion 86 and two through holes 84, the two through holes 84 include an input hole and an output hole for inputting and outputting the testing liquid; the extended rim 82 covers the periphery of the seat body 81, the seat body 81 is recessed to form a groove 86 with a circular periphery 87, and the two through holes 84 are located at the periphery of the groove 86 and are separated by 180 degrees.
Therefore, the fourth embodiment is also the same as the first embodiment, and the test liquid can smoothly flow to the sensing region of the biochip before testing the biochip by the structure of the circular groove portion 86, thereby avoiding turbulence caused by corner effect and further generating many bubbles to affect the subsequent test results. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and bubbles are prevented from remaining between the elastic buffer seat 8 and the surface of the biochip to further pollute the subsequent test flow.
Referring to fig. 8, a perspective view of an elastic buffer base for biochip test according to a fifth preferred embodiment of the present invention, the elastic buffer base 6 for biochip test of the fifth embodiment has substantially the same structure as the first embodiment, and the difference is that the bottom surface 661 of the groove 66 of the elastic buffer base 6 of the fifth embodiment has an arc-shaped protrusion 6611, but the bottom surface 73 and the top surface of the groove 76 of the buffer base body 71 of the first embodiment are horizontal. That is, the elastic buffer base 6 of the sixth embodiment includes: a buffer seat body 61 and an extended outer edge 62, the buffer seat body 61 has a groove 66 and two through holes 64, the two through holes 64 include an input hole and an output hole, the top surface 663 is horizontal, and the arc-shaped protrusion 6611 is disposed on the bottom surface 661 of the groove 66, and the arc-shaped protrusion 6611 is adjacent to the output holes of the two through holes 64. When testing the biochip, a liquid input tube 68 and a liquid output tube 69 are formed respectively for inputting and outputting the testing liquid; the extended outer edge 62 is disposed on the periphery 662 of the groove 66, and the buffer base body 61 forms an outwardly convex arc-shaped groove 66, and the two through holes 64 are respectively disposed at two corresponding ends of the periphery 662 of the groove 66.
Therefore, the fifth embodiment is also the same as the first embodiment, and the elliptical groove 66 structure can make the testing liquid smoothly flow to the sensing region of the biochip before testing the biochip, so as to avoid turbulence caused by corner effect and further generate many bubbles to affect the subsequent testing result. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and bubbles are prevented from remaining between the elastic buffer seat 6 and the surface of the biochip to further pollute the subsequent test flow.
Referring to fig. 9, a perspective view of an elastic buffer base for biochip test according to a sixth preferred embodiment of the present invention, the elastic buffer base 60 for biochip test according to the sixth embodiment has substantially the same structure as the fifth embodiment, except that a bottom 6061 of a groove portion 606 of the elastic buffer base 60 according to the sixth embodiment has an arc-shaped recess 60611, and one side of a bottom 661 of a groove portion 66 of the elastic buffer base 6 according to the fifth embodiment has an arc-shaped protrusion 6611. That is, the elastic buffer base 60 of the sixth embodiment includes: a buffer base body 601 and an extended outer edge 602, the buffer base body 601 has a groove 606 and two through holes 604, the two through holes 604 include an input hole and an output hole, the top surface 6063 is horizontal, and the arc-shaped recess 60611 is disposed on the bottom surface 661 of the groove 606, and the arc-shaped recess 60611 is adjacent to the output holes of the two through holes 604.
When testing the biochip, a liquid input tube 608 and a liquid output tube 609 are formed respectively for inputting and outputting the testing liquid; the extended rim 602 is disposed on the periphery 6062 of the buffer base body 601, and the buffer base body 601 forms a concave arc-shaped groove 606, and the two through holes 604 are respectively disposed at two corresponding ends of the periphery 6062 of the groove 606.
Therefore, the sixth embodiment is also the same as the first embodiment, and the elliptical groove 606 structure can make the testing liquid smoothly flow to the sensing region of the biochip before testing the biochip, so as to avoid turbulence caused by corner effect and further generate many bubbles to affect the subsequent testing result. And after the test is finished, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and bubbles are prevented from remaining between the elastic buffer seat 60 and the surface of the biochip to further pollute the subsequent test flow.
Please refer to fig. 10 to 12, which are an exploded view, a perspective cross-sectional view and a perspective view from another perspective of a testing module of a biochip according to a preferred embodiment of the invention. The drawing shows a biochip test module 1, which includes a holder 2, a hollow tube 3 and a probe 4. The fixing base 2 includes a fixing base body 22, a stop base 23 locked to the fixing base body 22, and a through hole 21, and a probe card 20 is fixed at the bottom of the fixing base 2, so as to perform a functional test on the biochip.
In the embodiment, the hollow sleeve 3 passes through the through hole 21 and the stop seat 23, and has a stop portion 31, the stop portion 31 is limited in the accommodating space between the fixed seat 2 and a stop inner edge 231 of the stop seat 23, and two springs 30 are interposed therebetween, thereby providing a buffer mechanism that can effectively prevent the biochip from being damaged due to overpressure when the biochip is moved up and down.
In addition, the probe 4 includes a probe body 41 and an elastic buffer base 42, the probe body 41 is assembled on the hollow casing 3, and the elastic buffer base 42 is engaged with the probe body 41 and connected to a liquid inlet tube 51, a liquid outlet tube 52 and an electrode 53, so as to perform a detection operation on the biosensor. Through the above design, in order to construct a suitable environment, the biochip is tested under the contact with the testing liquid, therefore, the pressure head 4 with the elastic buffer base 42 is used to effectively slow down the pressure force when contacting with the biochip, and at the same time, the elastic buffer base 42 is a detachable kit, which can be disassembled and cleaned together with the liquid input tube 51, the liquid output tube 52 and the electrode 53 connected with the elastic buffer base, thereby improving the replaceability and convenience.
Referring to fig. 2 and 3 together, the testing module of the biochip shown in fig. 12 is similar to the elastic buffer base 7 for testing the biochip of the first embodiment, and the difference is that the elastic buffer base 42 of the present embodiment is provided with an electrode hole for passing the electrode 53 through, that is, the elastic buffer base 42 is also composed of a buffer base body and an extended outer edge 421, the buffer base body has a groove 422 and three through holes, the three through holes include an input hole, an output hole and an electrode hole, the extended outer edge 421 is disposed on the periphery of the buffer base body (i.e. the buffer base body 71 shown in fig. 3), and the buffer base body (i.e. the buffer base body 71 shown in fig. 3) is recessed to form a groove 422 (i.e. the groove 76 shown in fig. 3) with an arc-shaped periphery, the input hole and the output hole are respectively disposed on the two corresponding ends of the periphery of the groove 422, the electrode hole is located in the middle of the recessed portion 422. The groove 422 is a small groove with a depth of 1 mm-3 mm, and the opening of the groove is required to cover the sensing area on the biochip, so as to create a detection environment capable of storing the test solution, and the electrode 53 is used to provide a reference voltage, which is beneficial for the test module to accurately measure the sensing signal of the biochip. In addition, the extension outer edge 421 of the embodiment is an extension outer edge extending to the periphery outside the outer wall at the bottom of the elastic buffer seat 42, and the extension outer edge 421 lengthens the leakage distance of the test solution flowing out, so that the problem that the test solution can be extruded to an external circuit board or electrode together when the elastic buffer seat 42 is deformed by stress can be avoided.
Furthermore, the material used for the elastic buffer seat 42 is different from the other main structural bodies, and is made of an elastic material such as Polydimethylsiloxane (PDMS) through mold turning. In addition, the elastic buffer seat 42 is easy to manufacture and low in cost, so that when damaged or deformed during the pressing process, the elastic buffer seat can be replaced together with the liquid input tube 51, the liquid output tube 52 and the electrode 53 connected with the elastic buffer seat, and the durability of the testing module of the biochip of the invention is improved through a replaceable consumable material design mode.
In addition, the elastic buffer base 42 of the present embodiment is similar to the elastic buffer base 7 for testing a biochip of the first embodiment, and the testing liquid can smoothly flow to the sensing region of the biochip before testing the biochip through the elliptical groove portion 422 structure, so as to avoid turbulence caused by corner effect and further generate many bubbles to affect the subsequent testing result. After the test is completed, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and air bubbles are prevented from remaining between the elastic buffer seat 42 and the surface of the biochip, so that the subsequent test flow is polluted.
Please refer to fig. 13 and 14, which are a perspective view and a cross-sectional view of a testing apparatus for a biochip according to a preferred embodiment of the present invention, and also refer to fig. 10-12. Fig. 13 shows a biochip Test apparatus 10 including a Test Head (Test Head)11, a biochip Test module 1, and a Prober (Prober) 13. The biochip test apparatus 10 is a machine apparatus in which the test module 1 is combined with a test head 11 and a prober 13. Therefore, the characteristics of the test module 1 for biochips are described in the previous paragraphs, and will not be described in detail here.
In the embodiment, the testing module 1 of the biochip is assembled on the prober 13, and the prober 13 is combined with the probe card 20 on the testing module 1, so that the biochip 12 placed on the movable stage 131 in the prober 13 can adjust the testing position of the biochip 12 by the movable stage 131, and the biochip 12 performs the testing solution and electrical property tests on the testing module 1 and the probe card 20 on the prober 13, and the signal line 15 is connected with the probe card 20 and the testing circuit board 14 of the testing head 11, so as to provide real-time transmission and calculation for the testing signal, thereby achieving the purpose of fully automatically testing the biochip 12 and effectively improving the testing efficiency.
Fig. 13 shows a biochip test apparatus, the elastic buffer base 42 is composed of a buffer base body and an extended outer edge 421, the buffer base body has a groove 422 and three through holes, the three through holes include an input hole, an output hole and an electrode hole, the extended outer edge 421 is disposed on the periphery of the buffer base body, the buffer base body is recessed to form a groove 422 with a circular arc-shaped periphery, the input hole and the output hole are respectively located at two corresponding ends of the periphery of the groove 422, and the electrode hole is located in the middle of the groove 422. The groove 422 is a small groove with a depth of 1 mm-3 mm, and the opening of the groove is required to cover the sensing area on the biochip, so as to create a detection environment capable of storing the test solution, and the electrode 53 is used to provide a reference voltage, which is beneficial for the test module to accurately measure the sensing signal of the biochip. In addition, the extension outer edge 421 of the embodiment is an extension outer edge extending to the periphery outside the outer wall at the bottom of the elastic buffer seat 42, and the extension outer edge 421 lengthens the leakage distance of the test solution flowing out, so that the problem that the test solution can be extruded to an external circuit board or electrode together when the elastic buffer seat 42 is deformed by stress can be avoided.
Furthermore, the material used for the elastic buffer seat 42 is different from the other main structural bodies, and is made of an elastic material such as Polydimethylsiloxane (PDMS) through mold turning. In addition, the elastic buffer seat 42 is easy to manufacture and low in cost, so that when damaged or deformed during the pressing process, the elastic buffer seat can be replaced together with the liquid input tube 51, the liquid output tube 52 and the electrode 53 connected with the elastic buffer seat, and the durability of the testing module of the biochip of the invention is improved through a replaceable consumable material design mode.
In addition, the elastic buffer base 42 of the present embodiment is similar to the elastic buffer base 7 for testing a biochip of the first embodiment, and the testing liquid can smoothly flow to the sensing region of the biochip before testing the biochip through the elliptical groove portion 422 structure, so as to avoid turbulence caused by corner effect and further generate many bubbles to affect the subsequent testing result. After the test is completed, the surface tension of the test liquid can be overcome, the test liquid can be smoothly extracted, and the test liquid and air bubbles are prevented from remaining between the elastic buffer seat 42 and the surface of the biochip, so that the subsequent test flow is polluted.
Through the design, the testing device of the biochip can accurately press and contact the pressure measuring head 4 and the biochip 12 in the testing process, so that the sensing area on the biochip 12 can be completely covered in the elastic buffer seat 42, the fluidity of the testing liquid in the elastic buffer seat 42 is kept, and the testing operation is carried out in the environment containing the solution.
The above-mentioned embodiments are merely for illustrative purposes, and the scope of the claims of the present invention should not be limited to the above-mentioned embodiments.
Claims (29)
1. An elastic buffer base for biochip test, comprising:
a buffer seat body, which is provided with a groove part and two through holes, wherein the two through holes comprise an input hole and an output hole; and
an extended outer edge disposed on the periphery of the buffer seat body, and the buffer seat body is recessed to form a groove portion with an arc-shaped periphery, and the two through holes are respectively disposed at two ends of the groove portion.
2. The elastic buffer base for biochip test as claimed in claim 1, wherein the recessed portion is oval, and the two through holes are located at the short arc portion of the recessed portion respectively.
3. The elastic buffer base for biochip test as claimed in claim 1, wherein the recessed portion is circular and the two through holes are separated by 180 degrees.
4. The elastic buffer seat for biochip test as defined in claim 1, wherein each through hole is formed through the recessed portion and forms a flange to form a design extending the output hole and the input hole for easy extraction of test liquid.
5. The elastic buffer base for biochip test as set forth in claim 1, wherein the groove portion of the buffer base body has a bottom surface in a horizontal shape.
6. The elastic buffer base for biochip test as defined in claim 1, wherein the recessed portion of the buffer base body has an inclined bottom surface extending to the output hole.
7. The elastic buffer seat for biochip test as defined in claim 1, wherein the recessed portion of the buffer seat body has an arc-shaped protrusion disposed on the bottom surface of the recessed portion, the arc-shaped protrusion being adjacent to the output hole.
8. The elastic buffer base for biochip test as defined in claim 1, wherein the recessed portion of the buffer base body has an arc-shaped recess disposed at the bottom of the recessed portion, the arc-shaped recess being adjacent to the output hole.
9. The elastic buffer base for biochip test according to claim 1, wherein the elastic buffer base is a one-piece structure made of Polydimethylsiloxane (PDMS).
10. The elastic buffer base for biochip test as defined in claim 1, wherein the side wall of the recessed portion has a rounded corner.
11. A test module of a biochip, comprising:
the fixing seat is provided with a through hole, and the bottom of the fixing seat is fixedly provided with a probe card;
a hollow sleeve passing through the through hole and arranged in the fixed seat in a penetrating way, at least two pipelines are arranged in the hollow sleeve in a penetrating way, the at least two pipelines comprise a fluid input pipe and a fluid output pipe, and the hollow sleeve is provided with a stopping part which is accommodated in the fixed seat and clamps a spring; and
the pressure measuring head comprises a pressure measuring head body and an elastic buffer seat, the pressure measuring head body is assembled on the hollow sleeve, and the elastic buffer seat is clamped in the pressure measuring head body and is connected with the at least two pipelines; the elastic buffer seat comprises a buffer seat body and an extended outer edge, the buffer seat body is provided with a groove part and two through holes, the two through holes comprise an input hole and an output hole, the extended outer edge is arranged on the periphery of the buffer seat body, the buffer seat body is concavely arranged to form the groove part with the periphery in an arc shape, and the two through holes are respectively positioned at two corresponding ends of the periphery of the groove part.
12. The biochip test module according to claim 11, wherein the slot is oval, and the two through holes are located at the short arc portion of the slot.
13. The biochip test module according to claim 11, wherein the recess is circular and the two through holes are spaced apart by 180 degrees.
14. The biochip measuring module according to claim 11, wherein each through hole is formed through the recessed portion and forms a flange to extend the output hole and the input hole, thereby facilitating the extraction of the test liquid.
15. The biochip test module according to claim 11, wherein the groove of the buffer base body has a bottom surface having a horizontal shape.
16. The testing module for biochip of claim 11 wherein the recessed portion of the buffer base body has an inclined bottom surface extending to the output hole.
17. The testing module for biochip according to claim 11 wherein the recessed portion of the buffer base body has an arcuate protrusion disposed on a bottom surface of the recessed portion, the arcuate protrusion being adjacent to the output hole.
18. The testing module for biochip according to claim 11 wherein the recessed portion of the buffer base body has an arc-shaped recess disposed at a bottom surface thereof, the arc-shaped recess being adjacent to the output hole.
19. The testing module for biochip of claim 11 wherein the buffer base body further has an electrode hole, the hollow sleeve is provided with an electrode through which the electrode is inserted.
20. The biochip test module according to claim 11, wherein the side wall of the recess of the buffer base body has a rounded corner.
21. A biochip measuring apparatus, comprising:
a probe tester having a movable stage for carrying a biochip;
the test module is arranged on the movable carrying platform and comprises a fixed seat, a hollow sleeve and a pressure measuring head, wherein the fixed seat is provided with a through hole, and the bottom of the fixed seat is fixedly provided with a probe card; the hollow sleeve penetrates through the through hole and is arranged in the fixed seat in a penetrating way, wherein at least one pipeline is arranged in the hollow sleeve in a penetrating way; the pressure measuring head comprises a pressure measuring head body and an elastic buffer seat, the pressure measuring head body is arranged on the hollow sleeve, the elastic buffer seat is clamped in the pressure measuring head body and is connected with the at least one pipeline, the elastic buffer seat comprises a buffer seat body and an extended outer edge, the buffer seat body is provided with a groove part and two through holes, the two through holes comprise an input hole and an output hole, the extended outer edge is arranged on the periphery of the buffer seat body, the buffer seat body is concavely provided with the groove part with an arc-shaped periphery, and the two through holes are respectively positioned at two corresponding ends of the periphery of the groove part; and
and the test head is electrically connected with the probe card and is provided with at least one test circuit board.
22. The biochip measuring apparatus according to claim 21, wherein the recess portion is oval, and the two through holes are located at the short arc portion of the recess portion.
23. The apparatus for testing a biochip of claim 21, wherein the recessed portion has a circular shape, and the two through holes are spaced apart by 180 degrees.
24. The apparatus for testing biochip according to claim 21, wherein each through hole is formed through the recessed portion and a flange is formed thereon, so as to form a design extending the output hole and the input hole, thereby facilitating the extraction of the testing liquid.
25. The biochip measuring apparatus, according to claim 21, wherein the recess portion of the buffer base body has a bottom surface having a horizontal shape.
26. The testing apparatus for biochips according to claim 21, wherein the recessed portion of the buffer base body has an inclined bottom surface extending to the output hole.
27. The testing apparatus for biochip according to claim 21, wherein the recess of the buffer base body has an arcuate projection provided on a bottom surface thereof, the arcuate projection being adjacent to the output hole.
28. The testing apparatus for biochips according to claim 21, wherein the recessed portion of the buffer base body has an arc-shaped recess provided at a bottom surface thereof, the arc-shaped recess being adjacent to the output hole.
29. The testing apparatus for biochips according to claim 21, wherein the side wall of the recess portion of the buffer base body has a rounded corner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811413452.7A CN111220767A (en) | 2018-11-23 | 2018-11-23 | Elastic buffer seat for testing biochip, testing module and testing equipment thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811413452.7A CN111220767A (en) | 2018-11-23 | 2018-11-23 | Elastic buffer seat for testing biochip, testing module and testing equipment thereof |
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| CN111220767A true CN111220767A (en) | 2020-06-02 |
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| CN201811413452.7A Pending CN111220767A (en) | 2018-11-23 | 2018-11-23 | Elastic buffer seat for testing biochip, testing module and testing equipment thereof |
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