CN111184775A - Feed additive and application thereof in resisting carp herpesvirus - Google Patents

Feed additive and application thereof in resisting carp herpesvirus Download PDF

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Publication number
CN111184775A
CN111184775A CN202010130542.6A CN202010130542A CN111184775A CN 111184775 A CN111184775 A CN 111184775A CN 202010130542 A CN202010130542 A CN 202010130542A CN 111184775 A CN111184775 A CN 111184775A
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parts
feed additive
feed
cyprinid
herpesvirus
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余乃通
刘志昕
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Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
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Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/68—Plantaginaceae (Plantain Family)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/19—Acanthaceae (Acanthus family)
    • A61K36/195—Strobilanthes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315—Isatis, e.g. Dyer's woad
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/344—Codonopsis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/634—Forsythia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A61P31/22—Antivirals for DNA viruses for herpes viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

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  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Food Science & Technology (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract

The invention discloses a feed additive and application thereof in resisting cyprinid herpesvirus, relates to the technical field of aquaculture, and solves the problems that cyprinid herpesvirus is high in frequency and mortality, so that relatively high risk exists in cyprinid fish culture and relatively high economic loss is brought to farmers, and the technical scheme is as follows: the feed additive mainly comprises the following raw materials: semen plantaginis, radix Puerariae, zeolite powder, Poria, radix Isatidis, Eucommiae cortex, radix Codonopsis, Atractylodis rhizoma, fructus forsythiae, bean crop, and green vegetable; the feed additive and the basic feed are mixed and then the cyprinid fish is fed for a long time, so that the immunity of the cyprinid fish to the cyprinid herpesvirus can be enhanced, the probability of the cyprinid herpesvirus is effectively reduced, the survival rate of the cyprinid fish infected with the cyprinid herpesvirus is improved, and basic technical conditions are provided for popularization and culture of the cyprinid fish.

Description

Feed additive and application thereof in resisting carp herpesvirus
Technical Field
The invention relates to the technical field of aquaculture, in particular to a feed additive and application thereof in resisting carp herpesvirus.
Background
The cyprinid herpesvirus disease is a highly pathogenic disease causing cyprinid and koi, has extremely strong infectivity, is popular in the world at present, and has the mortality rate of 75-100 percent. Cyprinid herpesvirus disease mainly infects cyprinus carpio and koi, and is now spread throughout the world in major aquaculture countries. Koi, carp and hybrids are highly sensitive to herpesvirus carpi, and other fishes of the family Cyprinidae (such as domestic fish) are not reported to be infected with herpesvirus carpi.
The pathogen of cyprinid herpesvirus disease is cyprinid herpesvirus type III (CyHV-3), belonging to the family Herpesviridae (Herpesviridae) and the genus cyprinid herpesvirus (Cyprinivirus). CyHV-3 is a spherical virus with mature virus particles, 170-230 nm in diameter. The nucleocapsid of the virus is a symmetrical decahedron, the diameter of the nucleocapsid is 100-110 nm, the virus consists of 32 virus polypeptides, genetic materials are double-stranded DNA, and the size of a genome is about 277 kb.
The cyprinid herpesvirus is spread rapidly, water is a main non-biological carrier for spreading the cyprinid herpesvirus, virus particles are discharged and spread through excrement, urine, gill and skin mucus of diseased fish, and the cyprinid herpesvirus is transmitted to other healthy fish, so that the cyprinid herpesvirus outbreak occurs. Cyprinid herpesvirus can infect koi and carp of any age but not other fish in different ponds. The optimal temperature for disease attack is 23-28 ℃, large-scale diseases and death can occur in the environment, but the death cannot be caused when the water temperature is lower than 18 ℃ or higher than 30 ℃. The disease mostly occurs in spring and autumn, the latent period is 14 days, the disease begins to die 24-48 hours after the disease is symptomatic, and the death rate rapidly rises within 2-4 days and can reach 80-100%.
The cyprinid herpesvirus is frequently infected and the mortality rate is high, so that the cyprinid fish culture has high risk, and great economic loss is brought to farmers. The research shows that the immunity of the fish body is improved, and the death caused by virus infection can be greatly reduced. Therefore, designing a feed additive capable of effectively reducing the incidence rate of cyprinid herpesvirus of cyprinid fishes and enhancing the immunity of cyprinid herpesvirus to cyprinid herpesvirus is a problem which needs to be solved urgently at present.
Disclosure of Invention
The invention aims to provide a feed additive, which is used for feeding cyprinid fishes for a long time after being mixed with a basic feed, can enhance the immunity of the cyprinid fishes to cyprinid herpesvirus, effectively reduce the probability of the cyprinid herpesvirus occurrence, improve the survival rate of the cyprinid fishes infected with the cyprinid herpesvirus occurrence, and provide basic technical conditions for popularization and culture of the cyprinid fishes.
The technical purpose of the invention is realized by the following technical scheme: a feed additive mainly comprises the following raw materials: semen plantaginis, radix Puerariae, zeolite powder, Poria, radix Isatidis, Eucommiae cortex, radix Codonopsis, Atractylodis rhizoma, fructus forsythiae, bean crop, and green vegetable.
Preferably, the additive is prepared from the following raw materials in parts by weight: 15-20 parts of semen plantaginis, 8-10 parts of radix puerariae, 0.8-1.2 parts of zeolite powder, 6-8 parts of poria cocos, 4-6 parts of radix isatidis, 10-12 parts of eucommia ulmoides, 6-8 parts of codonopsis pilosula, 8-10 parts of bighead atractylodes rhizome, 12-15 parts of fructus forsythiae, 20-30 parts of bean crops and 15-20 parts of green vegetables.
Preferably, the additive is prepared from the following raw materials in parts by weight: 20 parts of plantain seed, 10 parts of kudzu root, 1 part of zeolite powder, 6 parts of poria cocos, 6 parts of isatis root, 10 parts of eucommia bark, 8 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 15 parts of fructus forsythiae, 25 parts of bean crops and 15 parts of green vegetables.
Preferably, the bean crops comprise the following raw materials in parts by weight: 10-15 parts of soybeans, 10-15 parts of peas and 3-5 parts of mung beans.
Preferably, the green vegetable is one or a combination of more than two of spinach, lettuce and cabbage.
The invention also provides application of the feed additive in preparation of a carp herpesvirus resisting medicine.
The invention also provides application of the feed additive in preparing feed for resisting the carp herpesvirus and improving the immune function.
Preferably, the feed additive and the basal feed are mixed according to the following weight ratio of (1-2): 80 in proportion.
Preferably, the feed additive and the basic feed are mixed according to the weight ratio of 1: 50 in proportion.
In conclusion, the invention has the following beneficial effects:
(1) effectively inhibiting the proliferation and the effect of the cyprinid herpesvirus;
(2) the occurrence of symptoms such as skin bleeding after the skin is rubbed and broken by infected fish bodies and the capillaries are broken is reduced, and the situations such as massive bleeding of the capillaries, breakdown of a coagulation system of the fancy carp, exhaustion of multiple organs and death are effectively prevented;
(3) effectively inhibiting the occurrence of bleeding, local ulcer, scale loosening, scale falling and the like on the body surface of the diseased fish, and simultaneously having the detumescence effect on the red and swollen anus and the swollen kidney;
(4) can improve blood coagulation factor in blood coagulation system, and has physiological effects of reducing massive hemorrhage, and promoting normal blood coagulation.
(5) Can purify the breeding water quality, thereby reducing the infection probability of the herpes viruses of the carps.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more apparent, the present invention is further described in detail below with reference to the following embodiments. It is to be understood that the embodiments described are only a few embodiments of the present application and not all embodiments.
Selecting 6 aquaculture base ponds, wherein the ponds are A, B, C, D, E, F respectively, and the area of each aquaculture base pond is 50 square meters (m)2) 250 carps (body length 9.5 +/-2 cm) are cultured in each culture base pond, the average water depth is 2m, each culture base pond is averagely divided into five sub-ponds, and each sub-pond is internally provided with a net cage with the specification of 1.5m multiplied by 2.0m (length multiplied by width multiplied by height).
Example 1
Feeding the feed containing the feed additive in the pond A of the breeding base according to a conventional breeding method. The feed additive is prepared from the following raw materials in parts by weight: 15 parts of plantain seed, 8 parts of kudzu root, 0.8 part of zeolite powder, 6 parts of poria cocos, 4 parts of isatis root, 10 parts of eucommia bark, 6 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 12 parts of fructus forsythiae, 20 parts of bean crops and 15 parts of green vegetables.
The bean crops comprise the following raw materials in parts by weight: 10 parts of soybeans, 10 parts of peas and 3 parts of mung beans.
The green vegetable is spinach.
The feed additive and the basic feed are mixed according to the weight ratio of 1: 80 in proportion.
Example 2
Feeding the feed containing the feed additive in the pond B of the breeding base according to a conventional breeding method. The feed additive is prepared from the following raw materials in parts by weight: 20 parts of plantain seed, 10 parts of kudzu root, 1.2 parts of zeolite powder, 8 parts of tuckahoe, 6 parts of isatis root, 12 parts of eucommia bark, 8 parts of codonopsis pilosula, 10 parts of bighead atractylodes rhizome, 15 parts of forsythia, 30 parts of bean crops and 20 parts of green vegetables.
The bean crops comprise the following raw materials in parts by weight: 15 parts of soybeans, 15 parts of peas and 5 parts of mung beans.
The green vegetable is lettuce.
The feed additive and the basic feed are mixed according to the weight ratio of 1: 40 in proportion.
Example 3
Feeding the feed containing the feed additive in the pond C of the breeding base according to a conventional breeding method. The feed additive is prepared from the following raw materials in parts by weight: 15 parts of plantain seed, 10 parts of kudzuvine root, 0.8 part of zeolite powder, 8 parts of tuckahoe, 4 parts of isatis root, 12 parts of eucommia bark, 6 parts of codonopsis pilosula, 10 parts of bighead atractylodes rhizome, 12 parts of weeping forsythia, 30 parts of bean crops and 15 parts of green vegetables.
The bean crops comprise the following raw materials in parts by weight: 10 parts of soybeans, 15 parts of peas and 3 parts of mung beans.
The green vegetable is caulis et folium Brassicae Capitatae.
The feed additive and the basic feed are mixed according to the weight ratio of 1: 50 in proportion.
Example 4
In the pond D of the cultivation base, the feed containing the feed additive is fed according to the conventional cultivation method. The feed additive is prepared from the following raw materials in parts by weight: 20 parts of plantain seed, 8 parts of kudzu root, 1.2 parts of zeolite powder, 6 parts of poria cocos, 6 parts of isatis root, 10 parts of eucommia bark, 8 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 15 parts of fructus forsythiae, 20 parts of bean crops and 20 parts of green vegetables.
The bean crops comprise the following raw materials in parts by weight: 15 parts of soybeans, 10 parts of peas and 5 parts of mung beans.
The green vegetable is one or more of herba Spinaciae, lettuce, and caulis et folium Brassicae Capitatae.
The feed additive and the basic feed are mixed according to the weight ratio of 1: 50 in proportion.
Example 5
Feeding the feed containing the feed additive in the pond E of the breeding base according to a conventional breeding method. The feed additive is prepared from the following raw materials in parts by weight: 20 parts of plantain seed, 10 parts of kudzu root, 1 part of zeolite powder, 6 parts of poria cocos, 6 parts of isatis root, 10 parts of eucommia bark, 8 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 15 parts of fructus forsythiae, 25 parts of bean crops and 15 parts of green vegetables.
The bean crops comprise the following raw materials in parts by weight: 15 parts of soybeans, 10 parts of peas and 5 parts of mung beans.
The green vegetables are spinach, lettuce and Chinese cabbage according to the weight ratio of 1: 1: 1, preparation.
The feed additive and the basic feed are mixed according to the weight ratio of 1: 50 in proportion.
Experimental control group:
and feeding the feed containing no feed additive in the pond F of the culture base according to a conventional culture method.
And (4) experimental conclusion: the feeding environment and the feed quality of the control group and the experimental group are strictly kept consistent, 50 fish bodies are selected from each group on the 7 th day and the 25 th day respectively, DNA is extracted from gills and muscles of the fish bodies, then PCR detection is carried out, and the death rate is counted on the 25 th day. The results show that: after the fish fed with the feed containing the feed additive is fed, the probability of carp herpes virus infection is 11-14% on day 7, and is obviously reduced compared with the control group F (infection rate is 32%); further, the carp herpes virus infection probability of the fish fed with the feed additive-containing feed on day 25 is 39-47%, which is obviously reduced compared with that of a control group F (infection rate of 81%); mortality statistics were performed on the remaining 100 carps per group on day 25 and showed that mortality in fish fed with the feed additive was 30-39% at day 25, which was much lower than 70% of the control group. The detection results are shown in table 1:
TABLE 1 statistics of herpesvirus infections and cure rates for fish carp
Group of A B C D E F
Day 7 infection Rate (%) 12％ 13％ 12％ 14％ 11％ 32％
Infection rate on day 25 (%) 43％ 41％ 39％ 47％ 42％ 81％
Mortality on day 25 (%) 33％ 37％ 34％ 39％ 30％ 70％
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims (9)

1. A feed additive is characterized by mainly comprising the following raw materials: semen plantaginis, radix Puerariae, zeolite powder, Poria, radix Isatidis, Eucommiae cortex, radix Codonopsis, Atractylodis rhizoma, fructus forsythiae, bean crop, and green vegetable.
2. The feed additive as claimed in claim 1, wherein the additive is prepared from the following raw materials in parts by weight: 15-20 parts of semen plantaginis, 8-10 parts of radix puerariae, 0.8-1.2 parts of zeolite powder, 6-8 parts of poria cocos, 4-6 parts of radix isatidis, 10-12 parts of eucommia ulmoides, 6-8 parts of codonopsis pilosula, 8-10 parts of bighead atractylodes rhizome, 12-15 parts of fructus forsythiae, 20-30 parts of bean crops and 15-20 parts of green vegetables.
3. The feed additive as claimed in claim 2, wherein the additive is prepared from the following raw materials in parts by weight: 20 parts of plantain seed, 10 parts of kudzu root, 1 part of zeolite powder, 6 parts of poria cocos, 6 parts of isatis root, 10 parts of eucommia bark, 8 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 15 parts of fructus forsythiae, 25 parts of bean crops and 15 parts of green vegetables.
4. The feed additive as claimed in claim 1, wherein the bean crop comprises the following raw materials in parts by weight: 10-15 parts of soybeans, 10-15 parts of peas and 3-5 parts of mung beans.
5. The feed additive according to claim 1, wherein the green vegetable is one or more of spinach, lettuce and cabbage.
6. Use of a feed additive according to any one of claims 1 to 5 in the manufacture of a medicament against herpes carp virus.
7. Use of a feed additive according to any one of claims 1 to 5 in the preparation of a feed for enhancing immune function against herpes carpi viruses.
8. The use of the feed additive according to claim 7 in the preparation of feed for resisting carp herpesvirus and improving immune function, wherein the feed additive and basal feed are mixed according to the following weight ratio (1-2): 80 in proportion.
9. The use of the feed additive according to claim 8 in the preparation of feed for resisting carp herpesvirus and improving immune function, wherein the feed additive and the basal feed are mixed according to the weight ratio of 1: 50 in proportion.
CN202010130542.6A 2020-02-28 2020-02-28 Feed additive and application thereof in resisting carp herpesvirus Pending CN111184775A (en)

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CN102845596A (en) * 2012-09-03 2013-01-02 杨高林 Omnivorous fish feed and preparation method thereof
CN104686862A (en) * 2015-03-20 2015-06-10 广东海大集团股份有限公司 Feed additives for preventing carassius auratus gibelio from being infected by carp herpes viruses
CN106616028A (en) * 2016-12-08 2017-05-10 西南大学 Viral disease preventing and curing feed additive for cyprinus carpio and preparation method thereof
CN107897576A (en) * 2017-11-21 2018-04-13 无锡三智生物科技有限公司 A kind of hybridized prussian carp feed for preventing carp herpesvirusⅡtype virus

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