CN111154706B - L-色氨酸产量提高的重组大肠杆菌及其构建方法与应用 - Google Patents
L-色氨酸产量提高的重组大肠杆菌及其构建方法与应用 Download PDFInfo
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- CN111154706B CN111154706B CN202010033337.8A CN202010033337A CN111154706B CN 111154706 B CN111154706 B CN 111154706B CN 202010033337 A CN202010033337 A CN 202010033337A CN 111154706 B CN111154706 B CN 111154706B
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/227—Tryptophan
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- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01071—Shikimate kinase (2.7.1.71)
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CN202010033337.8A CN111154706B (zh) | 2020-01-13 | 2020-01-13 | L-色氨酸产量提高的重组大肠杆菌及其构建方法与应用 |
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CN202010033337.8A CN111154706B (zh) | 2020-01-13 | 2020-01-13 | L-色氨酸产量提高的重组大肠杆菌及其构建方法与应用 |
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CN111154706A CN111154706A (zh) | 2020-05-15 |
CN111154706B true CN111154706B (zh) | 2021-05-11 |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102140431A (zh) * | 2010-12-21 | 2011-08-03 | 大成生化科技(松原)有限公司 | L-色氨酸基因工程菌,其构建方法以及使用其发酵生产l-色氨酸的方法 |
CN109486737A (zh) * | 2018-12-03 | 2019-03-19 | 江南大学 | 一种高产l-色氨酸的重组大肠杆菌及其构建方法 |
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2020
- 2020-01-13 CN CN202010033337.8A patent/CN111154706B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102140431A (zh) * | 2010-12-21 | 2011-08-03 | 大成生化科技(松原)有限公司 | L-色氨酸基因工程菌,其构建方法以及使用其发酵生产l-色氨酸的方法 |
CN109486737A (zh) * | 2018-12-03 | 2019-03-19 | 江南大学 | 一种高产l-色氨酸的重组大肠杆菌及其构建方法 |
Non-Patent Citations (4)
Title |
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Development of L-tryptophan production strains by defined genetic modification in Escherichia coli;Zhi-Jun Zhao;《J Ind Microbiol Biotechnol》;20110504;第1921-1929页 * |
Effect of gene knockouts of l-tryptophan uptake system on the production of l-tryptophan in Escherichia coli;Zhijun Zhao;《Process Biochemistry》;20111122;第340-344页 * |
Zhi-Jun Zhao.Development of L-tryptophan production strains by defined genetic modification in Escherichia coli.《J Ind Microbiol Biotechnol》.2011,第1921-1929页. * |
Zhijun Zhao.Effect of gene knockouts of l-tryptophan uptake system on the production of l-tryptophan in Escherichia coli.《Process Biochemistry》.2011,第340-344页. * |
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