CN111135219A

CN111135219A - Plant stem cell composition for dissolving thrombus and eliminating cancerated cell and tumor

Info

Publication number: CN111135219A
Application number: CN202010041360.1A
Authority: CN
Inventors: 李春祥
Original assignee: Haohern Stem Cell Biotechnology Co Ltd
Current assignee: Haohern Stem Cell Biotechnology Co Ltd
Priority date: 2020-01-15
Filing date: 2020-01-15
Publication date: 2020-05-12

Abstract

The invention discloses a plant stem cell composition for dissolving thrombus and eliminating canceration cells and tumors; it adopts camptotheca acuminata, catharanthus roseus, taxus chinensis and oldenlandia diffusa fruit as raw materials to form a cambium to be induced, any one or more of the crushed materials, the extracts and the culture solution thereof is taken, starch is added to form a mixture, the mixture is uniformly stirred, ethanol or distilled water is added to be used as a wetting agent to be stirred until the mixture is completely uniform, and soft materials or drinks or granules are prepared, or the mixture is sieved by a sieve to filter out the granules, the granules are dried and then added with sodium stearate to be uniformly mixed, and the dripping pills are prepared to be orally taken. The plant stem cell composition can stimulate the immune system, eliminate variant cells and tumors, prevent and treat cardiovascular and cerebrovascular diseases, awaken self-repairing ability and immunity by a few drops, reduce the incidence of sudden death and advanced cancer caused by infarction when used in an early period, prolong the life of a patient and maintain the life health.

Description

Plant stem cell composition for dissolving thrombus and eliminating cancerated cell and tumor

Technical Field

The invention relates to a plant stem cell composition for dissolving thrombus and eliminating cancerated cells and tumors, belonging to the technical field of application of plant stem cells in treating cancers.

Background

Cancer is one of the diseases that accounts for the greatest proportion of the causes of death among modern people, and normal cells are transformed into cancer cells due to gene mutations for various reasons, resulting in malignant tumors that do not follow normal cell differentiation and growth morphology. The existing methods for treating malignant tumor cancer mainly comprise three methods, including surgery, radiotherapy and chemotherapy, the surgery has the problems of great side effect, incapability of preventing metastasis, extremely high cost and increased economic burden of patients; radiotherapy is suitable for acute inflammatory diseases, positive or malignant tumors, endocrine dysfunction, allergic diseases and the like, is generally effectively used for malignant tumors consisting of rapidly dividing cells, can cause the weakening or loss of the function of normal tissues, can generate skin diseases at the treatment part after treatment, can cause serious side effects such as intelligent development delay or bone development disorder and the like for children with ongoing organ development, and has the problems of bitter pain of patients in the treatment process. Although chemotherapy is widely used to disturb the replication or metabolism of cancer cells, anticancer agents have side effects of acting not only on cancer cells but also exerting their toxicity to normal cells of patients. Most of the side effects of chemotherapy require hospitalization in severe cases or require the administration of analgesics for the treatment of pain, which increases patient suffering. Therefore, many studies have been made on the development of novel drugs and agents having anticancer activity capable of inhibiting the growth of cancer cells and killing cancer cells, and in particular, studies have been focused on the development of anticancer substances in natural products having less side effects. The present inventors have made extensive efforts to develop natural-derived anticancer compositions that can minimize the side effects of conventional anticancer agents and act on the mechanism of cancer progression to express excellent anticancer activity, and as a result, have finally confirmed that cancer cell killing activity can be expressed from homogeneous cell lines including camptotheca acuminata, vinca rosea, taxus chinensis, and oldenlandia diffusa, their disrupted products, their extracts, and their culture solutions.

Disclosure of Invention

The invention aims to provide a plant stem cell composition which can minimize the side effect of the existing cancer therapeutic agent, has the activity of preventing and treating cancers and is derived from natural substances so as to solve the problems of great toxic and side effect, pain increase of patients, high cost and the like.

The plant stem cell composition for dissolving thrombus, eliminating cancerated cell and tumor includes camptotheca acuminata, catharanthus roseus, taxus chinensis and oldenlandia diffusa as raw material, and its cambium is induced, and its morphological characteristics are that it uses a large amount of vacuoles as morphological characteristics, and any one or more of its broken matter, its extract and its culture solution are in undifferentiated state.

The composition comprises, by weight, 10-15% of camptotheca acuminata, 5-20% of catharanthus roseus, 20-50% of taxus chinensis, 10-30% of oldenlandia diffusa and auxiliary materials. The camptotheca acuminata has the effects of resisting cancer, clearing heat and killing insects, and the camptothecine contained in the camptotheca acuminata is a plant anticancer drug and can be used for treating gastric cancer, colon cancer, rectal cancer, bladder cancer, chronic myelocytic leukemia, acute lymphocytic leukemia and the like; catharanthus roseus is a plant of Apocynaceae, has a plurality of medicinal effects of relieving pain, diminishing inflammation, soothing the nerves, relaxing bowels, inducing diuresis and the like, also has a good treatment effect on malignant tumors and leukemia problems, and generally can play a certain role in inhibiting the recovery process of leukemia and tumor diseases; paclitaxel extracted from bark and leaves of Taxus chinensis has good therapeutic effect on various cancers of late stage; the oldenlandia diffusa has the functions of clearing away heat and toxic materials, promoting blood circulation and inducing diuresis, and the oldenlandia diffusa also contains polysaccharide components, so that the oldenlandia diffusa can help to eliminate free radicals in a body, enhance the immunity of a human body, and play a role in inhibiting the growth of tumor cells and preventing and resisting cancers.

The plant stem cell composition auxiliary material is starch, ethanol or distilled water, and the composition is formed into a mixture by adding starch.

The plant stem cell composition also has the following characteristics: it exists as a single cell during suspension culture; lower sensitivity to shear stress in the bioreactor compared to cell lines from tissues other than the ginseng cambium; has a higher growth rate and can be stably cultured as compared with cell lines derived from tissues other than ginseng type-forming layers.

Adding ethanol or distilled water as adjuvant as moisturizer, stirring the composition until uniform, making into soft preparation, such as dripping pill, sieving, filtering to obtain granule, oven drying, and making into dripping pill.

The mesh of the prepared dripping pill is 50-100 meshes.

The water content of the dried granules of the composition prepared into the dripping pill is 1-8%.

The temperature of the dryer for drying the particles is kept between 30 and 120 ℃.

The plant stem cell composition for dissolving thrombus and eliminating cancerated cells and tumors is prepared by taking camptotheca acuminata, catharanthus roseus, taxus chinensis and oldenlandia diffusa which have anticancer plant elements as raw materials, inducing cambium or procambium containing tissues of plants, culturing the obtained cambium or procambium containing tissues, inducing the cambium or procambium to proliferate to form a layer or procambium, and inducing an amorphous callus layer to proliferate from other tissues except the cambium or procambium; collecting cell line from formation layer or original formation layer, collecting crushed material, extract and culture solution thereof, adding starch, ethanol or distilled water, stirring, and making into beverage, or filtering with screen mesh to obtain granule, oven drying, and making into dripping pill. The plant stem cell composition can dissolve embolism substances on blood and blood vessels, particularly cysteine, achieve the effect of melting ice by hot water, stimulate an immune system to eliminate variant cells and tumors, distinguish medicines from a method of softening blood, treating cancer by operation and radiotherapy and chemotherapy, play a role in preventing and treating cancer, and prevent infarction, sudden death or late cancer incidence by early administration, thereby prolonging the life of a patient and maintaining the life health.

Detailed Description

Example 1: selecting 150g of camptotheca acuminata, 50g of catharanthus roseus, 200g of taxus chinensis and 100g of oldenlandia diffusa, preferentially grinding roots into powder, soaking the powder in a solvent for a period of time, wherein the solvent is 100mg/L of an antioxidant, then pretreating the plants for 24 hours by using a mixed solution of organic bactericides of 5% of organic sulfur (such as mancozeb), 3% of trichloromethionyl (such as captan) instead of chlorothalonil and 0.1% of amitraz-guanidine cephalosporin sodium, and then repeatedly washing the plants for 40 minutes by using tap water so as to remove phenolic compounds and residual chemical substances. Then, the plants were surface sterilized in 70% ethanol for 2 minutes, 30% hydrogen peroxide for 10 minutes, and then washed 3-4 times with water. Thus, the procambium and cambium were separated, the division of cells from the procambium and cambium was visually observed, and after 20 days of culture, amorphous callus formed by dedifferentiation began to be induced from the layer consisting of phloem, cortex and epidermis. However, during the whole culture process, no cell division occurs in the xylem, and thus the formation layer is naturally separated from the xylem. After culturing for 30 days, the tissue begins to be divided into a formation layer and an upper layer containing phloem, namely an amorphous callus layer, and after the tissue is completely and naturally divided into two layers, the layers are respectively cultured in different culture dishes to be proliferated and form a uniform cell layer, the cambium is induced, any one or more of a crushed product, an extract and a culture solution thereof is taken, 100g of starch is added and mixed, ethanol or distilled water is added as a wetting agent and stirred until the mixture is uniform, a soft material or a drink is prepared, for example, the soft material or the drink is prepared, particles can be filtered out by a sieve with 50 meshes and added with sodium stearate and dried by a dryer, the water content of the dried particles is 1-8 percent, and the temperature of the dried particles by the dryer is kept between 30 and 120 ℃. The using method comprises the following steps: the oral liquid is administered orally at 5-10 drops per day. The dripping pill is orally administered 2 times a day, 3-5 granules each time.

Example 2: the method is the same as that of example 1 except that the plants of camptotheca acuminata 100g, catharanthus roseus 200g, taxus chinensis 500g and oldenlandia diffusa 200g are selected.

Example 3: the method is the same as example 1 except that 120g of camptotheca acuminata, 150g of catharanthus roseus, 400g of taxus chinensis and 130g of oldenlandia diffusa are selected as plants.

Claims

1. A plant stem cell composition for dissolving thrombus and eliminating cancerated cells and tumors is characterized in that: the composition is prepared from Camptotheca acuminata, Catharanthus roseus, Taxus chinensis, and Hedyotis diffusa, and has cambium induced, large amount of vacuole as morphological feature, and one or more of its broken product, its extract and its culture solution, which are in undifferentiated state.

2. The plant stem cell composition for dissolving thrombus, eliminating cancerated cells and tumors according to claim 1, which is characterized in that: the composition comprises, by weight, 10-15% of camptotheca acuminata, 5-20% of catharanthus roseus, 20-50% of taxus chinensis, 10-30% of oldenlandia diffusa and auxiliary materials.

3. The plant stem cell composition for dissolving thrombus, eliminating cancerated cells and tumors according to claim 1, which is characterized in that: the composition adjuvant is starch, ethanol or distilled water.

4. The plant stem cell composition for dissolving thrombus, eliminating cancerated cells and tumors according to claim 1, which is characterized in that: the plant stem cell composition also has the following characteristics: it exists as a single cell during suspension culture; lower sensitivity to shear stress under the bioreactor compared to cell lines from tissues other than the ginseng-like layer; has a higher growth rate and can be stably cultured as compared with cell lines derived from tissues other than ginseng type-forming layers.

5. The plant stem cell composition for dissolving thrombus, eliminating cancerated cells and tumors according to claim 1, which is characterized in that: the composition can be mixed with ethanol or distilled water as humectant, and stirred to obtain soft preparation or beverage, such as dripping pill, filtering with screen to obtain granules, adding sodium stearate, oven drying, and making into dripping pill.

6. The preparation method of the plant stem cell composition for dissolving thrombus, eliminating cancerated cells and tumors according to claim 5 is characterized in that: the mesh of the prepared dripping pill is 50-100 meshes.

7. The preparation method of the plant stem cell composition for dissolving thrombus, eliminating cancerated cells and tumors according to claim 5 is characterized in that: the water content of the dried granules is 1-8%.

8. The preparation method of the plant stem cell composition for dissolving thrombus, eliminating cancerated cells and tumors according to claim 5 is characterized in that: the temperature of the dryer for drying the particles is kept between 30 and 120 ℃.