CN110980964B - Water treatment composite active microbial inoculum and application thereof - Google Patents
Water treatment composite active microbial inoculum and application thereof Download PDFInfo
- Publication number
- CN110980964B CN110980964B CN201911298481.8A CN201911298481A CN110980964B CN 110980964 B CN110980964 B CN 110980964B CN 201911298481 A CN201911298481 A CN 201911298481A CN 110980964 B CN110980964 B CN 110980964B
- Authority
- CN
- China
- Prior art keywords
- microbial inoculum
- bacillus
- water treatment
- composite active
- active microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002131 composite material Substances 0.000 title claims abstract description 46
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 241000894006 Bacteria Species 0.000 claims abstract description 30
- 239000010865 sewage Substances 0.000 claims abstract description 27
- 230000006698 induction Effects 0.000 claims abstract description 21
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 14
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 241000589516 Pseudomonas Species 0.000 claims abstract description 13
- 230000003213 activating effect Effects 0.000 claims abstract description 11
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 9
- 229920002101 Chitin Polymers 0.000 claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- 150000001413 amino acids Chemical class 0.000 claims abstract description 8
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 8
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims abstract description 8
- 239000008103 glucose Substances 0.000 claims abstract description 8
- 239000012138 yeast extract Substances 0.000 claims abstract description 8
- 239000004471 Glycine Substances 0.000 claims abstract description 7
- 241000108664 Nitrobacteria Species 0.000 claims abstract description 6
- 150000001875 compounds Chemical class 0.000 claims description 11
- 230000001546 nitrifying effect Effects 0.000 claims description 5
- 241000193738 Bacillus anthracis Species 0.000 claims description 2
- 241000194107 Bacillus megaterium Species 0.000 claims description 2
- 241000194103 Bacillus pumilus Species 0.000 claims description 2
- 244000063299 Bacillus subtilis Species 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 2
- 241000254158 Lampyridae Species 0.000 claims description 2
- 241000605159 Nitrobacter Species 0.000 claims description 2
- 241001495402 Nitrococcus Species 0.000 claims description 2
- 241000192147 Nitrosococcus Species 0.000 claims description 2
- 241000605122 Nitrosomonas Species 0.000 claims description 2
- 241001495394 Nitrosospira Species 0.000 claims description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims description 2
- 241000589540 Pseudomonas fluorescens Species 0.000 claims description 2
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 claims description 2
- 241000694486 Stewartia <clam> Species 0.000 claims description 2
- 229940065181 bacillus anthracis Drugs 0.000 claims description 2
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 claims 3
- 238000002360 preparation method Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000035899 viability Effects 0.000 abstract description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 239000002351 wastewater Substances 0.000 description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 6
- 229910052698 phosphorus Inorganic materials 0.000 description 6
- 239000011574 phosphorus Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- QMMFVYPAHWMCMS-UHFFFAOYSA-N Dimethyl sulfide Chemical compound CSC QMMFVYPAHWMCMS-UHFFFAOYSA-N 0.000 description 4
- WQOXQRCZOLPYPM-UHFFFAOYSA-N dimethyl disulfide Chemical compound CSSC WQOXQRCZOLPYPM-UHFFFAOYSA-N 0.000 description 4
- 230000000593 degrading effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000002957 persistent organic pollutant Substances 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000003124 biologic agent Substances 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007102 metabolic function Effects 0.000 description 2
- JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical compound N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 239000005842 Thiophanate-methyl Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- KHPLPBHMTCTCHA-UHFFFAOYSA-N ammonium chlorate Chemical compound N.OCl(=O)=O KHPLPBHMTCTCHA-UHFFFAOYSA-N 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000005272 metallurgy Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 125000001477 organic nitrogen group Chemical class 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 150000003071 polychlorinated biphenyls Chemical class 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- UKLNMMHNWFDKNT-UHFFFAOYSA-M sodium chlorite Chemical compound [Na+].[O-]Cl=O UKLNMMHNWFDKNT-UHFFFAOYSA-M 0.000 description 1
- 229960002218 sodium chlorite Drugs 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the technical field of sewage treatment, and particularly discloses a water treatment composite active microbial inoculum and application thereof. The water treatment composite active microbial inoculum is characterized in that: comprises a composite flora and an induction base which are mixed into a whole, wherein the composite flora consists of nitrobacteria, denitrifying bacteria, bacillus, pseudomonas, activating enzyme, photosynthetic bacteria and glycine bacteria; the induction group consists of amino acid, yeast extract, chitin, chitosan oligosaccharide and glucose. The product of the invention has the advantages of simple preparation, low production cost, strong flora viability, easy culture, fast propagation, strong adaptability to the environment, natural evolution and other characteristics, conforms to the requirement of sewage treatment and has strong applicability.
Description
Technical Field
The invention relates to the technical field of sewage treatment, and particularly relates to a water treatment composite active microbial inoculum and application thereof.
Background
In recent years, the amount of sewage discharged has been increasing due to frequent human activities. The sewage mainly comprises domestic sewage, breeding sewage and industrial wastewater. The components of the industrial sewage are relatively complex, particularly a large amount of artificially synthesized compounds enter the environment, the substances mainly comprise ammonia nitrogen, sulfide and phosphorus-containing compounds, and due to the complexity of the structures of the substances, the traditional wastewater treatment method cannot effectively remove the pollutants; in addition, with the acceleration of the social industrialization process, a large amount of organic pollutants which are difficult to degrade, such as polycyclic aromatic hydrocarbons, halogenated compounds, polychlorinated biphenyls, organic nitrogen and phosphorus pesticides and the like, are discharged into the environment, and most of the organic pollutants are artificially synthesized, so that the types and the number of microorganisms which have high-efficiency degradation functions in natural environment are few, and the organic pollutants are in a disadvantage in interspecific competition; in addition, main pollutants in the culture water area are feed residues, excrement and some medicines, and the accumulation of nitrogen and phosphorus nutrients in the feed can cause eutrophication of the water body and can also cause the serious standard exceeding of chemical oxygen demand, ammonia nitrogen, nitrite and the like in the water body.
At present, the sewage treatment technology in China basically takes the use of chemical preparations and the combination of aeration treatment as main materials, and the chemical preparations mostly take the combination of hydrochloric acid, sodium chlorite and ammonium chlorate as main materials. The application of strong acid and strong chlorine has certain sewage treatment application effect on one hand, but brings secondary pollution of sludge along with the application of the strong acid and the strong chlorine, the aeration is reinforced by the forced feeding of gas, the problem of 'standard' is solved, but the problem of permanent cure is solved, the more pollution and more odor problem is particularly serious, the actual case of treating sewage by using a simple biological agent does not really appear on sewage treatment, and the reason is that the biological agent is not strong enough for floras. The sewage treatment by biotechnology is a development direction, but the existence of everything in the world has the functions and significance of the existence of the sewage treatment agent, so that the development of a sewage treatment microbial inoculum with strong functions and convenient application is very important.
Disclosure of Invention
The invention provides a water treatment composite active microbial inoculum with strong applicability, wide application and low production cost and application thereof in order to make up for the defects of the prior art.
The invention is realized by the following technical scheme:
a water treatment composite active microbial inoculum comprises a composite flora and an induction base which are mixed into a whole, wherein the composite flora consists of 40-55% of nitrobacteria, 10-15% of denitrifying bacteria, 5-20% of bacillus, 5-10% of pseudomonas, 8-10% of activating enzyme, 7-10% of photosynthetic bacteria and 3-5% of glycine strains by mass; the induction group consists of 5-8% of amino acid, 5-10% of yeast extract, 1-2% of chitin, 0.1-0.3 per mill of chitosan oligosaccharide and 78-90% of glucose in percentage by mass.
The invention compounds a plurality of floras with different properties, combines with an induction group to provide growth energy for the complex floras, and can lead the complex floras to spontaneously or be induced to generate a new enzyme system according to the environment, thereby having new metabolic function and degrading or converting new compounds.
The more preferable technical scheme of the invention is as follows:
the dosage of the induction group is 15-20% of the mass of the composite flora, and the induction group plays a role in assisting and inducing in the growth process of the composite flora.
The composite flora consists of 50% of nitrobacteria, 12% of denitrifying bacteria, 10% of bacillus, 8% of pseudomonas, 8% of activating enzyme, 7% of photosynthetic bacteria and 5% of glycine bacteria by mass percent, and is a preferable proportion of the composite flora.
The induction group consists of 6 mass percent of amino acid, 8 mass percent of yeast extract, 2 mass percent of chitin, 0.2 per mill of chitosan oligosaccharide and 83.98 mass percent of glucose, and is a better proportion of the induction group.
In the invention, the induction group is used as the energy and growth source of the compound flora, so that the compound flora can generate a new enzyme system by spontaneous or induced generation gradually according to the environment of the compound flora, and has a new metabolic function, thereby degrading or converting a new compound, and enabling the compound flora to have more flexible autonomy so as to adapt to different sewage treatment environments.
The nitrifying bacteria are one or more of nitrosomonas, nitrosococcus, nitrosospira, nitrobacter, nitrococcus and nitrocystis; nitrifying bacteria, as a type of aerobic bacteria, mainly comprise nitrosobacteria and nitrobacteria, and play an important role in purifying nitrogen circulating water in an aerobic environment.
The denitrifying bacteria are one or more of denitrifying bacillus, stewartia and firefly aerothrix; denitrifying bacteria, acting as heterotrophic, facultative anaerobic bacteria, can cause denitrification.
The bacillus is one or more of bacillus thuringiensis, bacillus subtilis, bacillus pumilus, bacillus anthracis and bacillus megaterium, is a strictly aerobic or facultative anaerobic bacillus with a capsule and can generate spores with special resistance to adverse conditions; the pseudomonas is one or more of pseudomonas aeruginosa, pseudomonas fluorescens, pseudomonas gangrenia and pseudomonas pseudonares, and is obligate aerobic gram-negative spore-free and capsular bacillus; the activating enzyme is Rubisco activating enzyme.
The photosynthetic bacteria are gram-negative bacteria without the capability of forming spores, can perform photosynthesis by using organic matters, sulfides, ammonia and the like in nature as hydrogen donor and carbon source under the anaerobic illumination or aerobic dark condition by using light as energy, has certain tolerance and decomposition capability on toxicants such as phenol, cyanogen and the like, and has stronger decomposition and conversion capability.
The application of the water treatment composite active microbial inoculum in sewage treatment is suitable for sewage with various pH values, various temperatures and dissolved oxygen amounts.
The composite active microbial inoculum of the invention has the functions of degrading COD, BOD, ammonia nitrogen, total nitrogen and SS, and has obvious functions of deodorizing and manuscript-repairing and eliminating NH3、P、H2The S and the organic acid have super strong capability and strong ecological restoration capability, can inhibit viruses, germs and parasites, effectively inhibit the propagation of algae and purify water and water color.
The composite active microbial inoculum of the invention also has peculiar effect on low-concentration heavy metal pollution, such as zinc, manganese, iron, chromium, cadmium, lead and arsenic.
The product of the invention has the advantages of simple preparation, low production cost, strong flora viability, easy culture, fast propagation, strong adaptability to the environment, natural evolution and other characteristics, conforms to the requirement of sewage treatment and has strong applicability.
Detailed Description
The invention is further described below with reference to specific examples to facilitate the understanding of the invention. It is apparent that the following embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: a water treatment composite active microbial inoculum comprises the following components:
composite flora (mass fraction ratio):
40% of nitrifying bacteria, 10% of denitrifying bacteria, 20% of bacillus, 5% of pseudomonas, 10% of activating enzyme, 10% of photosynthetic bacteria and 5% of glycine strain;
induction base (mass fraction):
the mass fraction is 5% of amino acid, 5% of yeast extract, 1% of chitin, 0.1% o of chitosan oligosaccharide and 88.99% of glucose;
the dosage of the induction group is 20% of the mass of the composite flora, and the two are uniformly mixed into a whole to obtain the composite active microbial inoculum.
The method is characterized in that the wastewater produced by thiophanate methyl of a certain company is taken as a treatment object, wherein the COD concentration in the wastewater is 9000mg/L, the methyl sulfide is not detected, the concentration of dimethyl disulfide is 350mg/L, and the odor is large.
Taking 100L of the wastewater, adding 100g of the composite active microbial inoculum into the wastewater, standing for 72 hours, and detecting the treated wastewater, wherein the COD concentration of the wastewater is 4500mg/L, the dimethyl sulfide concentration is 0mg/L, the dimethyl disulfide concentration is 0mg/L, and the wastewater has no foul smell; and standing for 72 hours continuously, and detecting the wastewater, wherein the COD concentration of the wastewater is 150 mg/L.
In the composite active microbial inoculum, the photosynthetic bacteria and the bacillus can convert malodorous gas hydrogen sulfide into sulfur element required by growth of the composite active microbial inoculum, thereby achieving the aim of deodorization.
Example 2: a water treatment composite active microbial inoculum comprises the following components:
composite flora (mass fraction ratio):
55% of nitrobacteria, 15% of denitrifying bacteria, 5% of bacillus, 50% of pseudomonas, 10% of activating enzyme, 7% of photosynthetic bacteria and 3% of glycine strain;
induction base (mass fraction):
the mass fraction is 8% of amino acid, 10% of yeast extract, 2% of chitin, 0.3% o of chitosan oligosaccharide and 79.97% of glucose;
the dosage of the induction group is 15 percent of the mass of the composite flora, and the induction group and the composite flora are uniformly mixed into a whole to obtain the composite active microbial inoculum.
The waste water mainly contains inorganic salts such as sodium chloride, sodium sulfate, sodium carbonate and sodium bicarbonate, wherein Na is+≤20000mg/L，SO4 2-≤30000mg/L，Cl-≤20000mg/L，CO3 2-≤4000mg/L，HCO3 -Less than or equal to 2000mg/L, and other heavy metal ions all accord with the national industrial metallurgy wastewater discharge standard.
Taking 100L of the wastewater, adding 100g of the composite active microbial inoculum into the wastewater, standing for 72 hours, and detecting the treated wastewater, wherein Na is contained in the wastewater+≤5000mg/L，SO4 2-≤5000mg/L，Cl-≤4000mg/L，CO3 2-≤200mg/L，HCO3 -Less than or equal to 100mg/L and no heavy metal ions exist.
Example 3: a water treatment composite active microbial inoculum comprises the following components:
composite flora (mass fraction ratio):
50% of nitrifying bacteria, 12% of denitrifying bacteria, 10% of bacillus, 8% of pseudomonas, 8% of activating enzyme, 7% of photosynthetic bacteria and 5% of glycine strain;
induction base (mass fraction):
the mass fraction is 6% of amino acid, 8% of yeast extract, 2% of chitin, 0.2% o of chitosan oligosaccharide and 83.98% of glucose;
the dosage of the induction group is 18 percent of the mass of the composite flora, and the two are uniformly mixed into a whole to obtain the composite active microbial inoculum.
The domestic sewage is treated by taking the domestic sewage as a treatment object, the pH value of the domestic sewage is 6-9, the total phosphorus content is 4-15mg/L, the ammonia nitrogen content is 55-100mg/L, the COD concentration is 200-400mg/L, and the BOD concentration is 100-200 mg/L.
Taking 100L of the domestic sewage, adding 100g of the composite active microbial inoculum into the sewage, standing for 72 hours, and detecting the treated sewage, wherein the total phosphorus content is 0.65mg/L, the ammonia nitrogen content is 0.8-1mg/L, the COD concentration is 50mg/L, the BOD concentration is 15mg/L, and the pH value is 7.4; and continuously standing for 72 hours, and detecting the treated sewage, wherein the total phosphorus content is 0.35mg/L, the ammonia nitrogen content is 0.05-0.1mg/L, the COD concentration is 35mg/L, the BOD concentration is 11mg/L, and the pH value is 7.2.
In the composite active microbial inoculum of the invention, the participation of the Glycerin species can become dominant bacteria in the environment, and can inhibit the growth of pathogenic bacteria and putrefying bacteria (for example, after lactobacillus and the like become dominant bacteria, the growth of Escherichia coli and the like in the environment can be inhibited, thereby reducing the generation of odor such as ammonia gas and the like).
The above-mentioned embodiments are intended to illustrate the technical solutions and advantages of the present invention, and it should be understood that the above-mentioned embodiments are only specific embodiments of the present invention, and are not intended to limit the present invention, and any modifications, additions, equivalents, etc. made within the scope of the principles of the present invention should be included in the scope of the present invention.
Claims (6)
1. A water treatment composite active microbial inoculum is characterized in that: the composite microbial flora comprises a composite microbial flora and an induction base which are mixed into a whole, wherein the composite microbial flora consists of 50% of nitrobacteria, 12% of denitrifying bacteria, 10% of bacillus, 8% of pseudomonas, 8% of activating enzyme, 7% of photosynthetic bacteria and 5% of glycine genus; the induction group consists of 5-8% of amino acid, 5-10% of yeast extract, 1-2% of chitin, 0.1-0.3 per mill of chitosan oligosaccharide and 78-90% of glucose in percentage by mass; the dosage of the induction group is 15-20% of the mass of the compound flora.
2. The water treatment composite active microbial inoculum according to claim 1, which is characterized in that: the induction group consists of 6 mass percent of amino acid, 8 mass percent of yeast extract, 2 mass percent of chitin, 0.2 per mill of chitosan oligosaccharide and 83.98 mass percent of glucose.
3. The water treatment composite active microbial inoculum according to claim 1, which is characterized in that: the nitrifying bacteria are one or more of nitrosomonas, nitrosococcus, nitrosospira, nitrobacter, nitrococcus and nitrocystis.
4. The water treatment composite active microbial inoculum according to claim 1, which is characterized in that: the denitrifying bacteria are one or more of denitrifying bacillus, stewartia and firefly aerothrix.
5. The water treatment composite active microbial inoculum according to claim 1, which is characterized in that: the bacillus is one or more of bacillus thuringiensis, bacillus subtilis, bacillus pumilus, bacillus anthracis and bacillus megaterium; the Pseudomonas is one or more of Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas farci and Pseudomonas pseudofarci; the activating enzyme is Rubisco activating enzyme.
6. The use of the composite active microbial inoculum for water treatment according to claim 1 in sewage treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911298481.8A CN110980964B (en) | 2019-12-17 | 2019-12-17 | Water treatment composite active microbial inoculum and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911298481.8A CN110980964B (en) | 2019-12-17 | 2019-12-17 | Water treatment composite active microbial inoculum and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110980964A CN110980964A (en) | 2020-04-10 |
| CN110980964B true CN110980964B (en) | 2020-12-15 |
Family
ID=70094415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911298481.8A Active CN110980964B (en) | 2019-12-17 | 2019-12-17 | Water treatment composite active microbial inoculum and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110980964B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112573669A (en) * | 2020-11-20 | 2021-03-30 | 广州绵源环境技术有限公司 | Complex microbial inoculant |
| CN113023905A (en) * | 2021-03-31 | 2021-06-25 | 北京局气网络技术有限公司 | Plant fermentation extract for adjusting fresh water quality and preparation method thereof |
| CN113336340A (en) * | 2021-06-28 | 2021-09-03 | 狠牛(苏州)生物科技有限公司 | Fermentation additive for improving water quality and preparation method thereof |
| CN113428986A (en) * | 2021-07-05 | 2021-09-24 | 北京局气网络技术有限公司 | Additive for increasing oxygen content in water and preparation method thereof |
| CN113371850B (en) * | 2021-08-12 | 2021-11-12 | 山东得和明兴生物科技有限公司 | Compound microbial agent for efficiently purifying water and application thereof |
| CN114275906A (en) * | 2021-12-10 | 2022-04-05 | 安徽安泰农业开发有限责任公司 | Composite microbial inoculum for treating sewage of pig farm and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105198167A (en) * | 2015-10-22 | 2015-12-30 | 江苏大学 | Microbiological degradation water treatment system of industrial wastewater |
| CN110482786A (en) * | 2019-08-12 | 2019-11-22 | 广东长海建设工程有限公司 | The method of municipal sewage treatment |
-
2019
- 2019-12-17 CN CN201911298481.8A patent/CN110980964B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110980964A (en) | 2020-04-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110980964B (en) | Water treatment composite active microbial inoculum and application thereof | |
| CN108441444B (en) | Compound microbial agent suitable for black and odorous water treatment | |
| CN105368745B (en) | Composite microbial preparation for treating black and odorous rivers and preparation method thereof | |
| CN105217802B (en) | A kind of water quality cleansing agent | |
| CN101434907B (en) | Microbial preparation for processing refuse leachate and preparation | |
| CN101705202A (en) | Pseudomonas stutzeri YZN-001 for removing ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application | |
| CN103497908B (en) | Pseudomonas stutzeri and cultivation, immobilization and application | |
| CN102531195B (en) | A kind of autotrophic denitrification water quality regulation bacterium agent and application thereof | |
| CN105967340A (en) | Ecological restoration method for eutrophic river channel water body | |
| CN107699513B (en) | Black and odorous water body degrading bacterium and application thereof | |
| CN102533582A (en) | Efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and application thereof | |
| US6328891B1 (en) | Process for the biological purification of a water containing ammonium perchlorate | |
| CN103275908B (en) | Low-temperature denitrification pseudomonas fluorescens | |
| CN104830740A (en) | Preparation and application methods of efficient microorganism agent for treating compound fertilizer wastewater | |
| CN111689587A (en) | Method for treating river channel through microbial integrated ecological restoration and application of method | |
| Lahiri et al. | Biogeochemical cycling bacteria and nutrient dynamics in waste stabilization pond system | |
| Qu et al. | The study of natural biofilm formation and microbial community structure for recirculating aquaculture system | |
| CN104388342B (en) | A kind of short distance nitration bacterium pseudomonad and application | |
| CN108558001B (en) | Biological activator for in-situ remediation of polluted water and bottom mud and use method thereof | |
| Hao | Sulphate-reducing bacteria | |
| CN103381418A (en) | Method for processing tobacco waste or organic fluorine wastewater | |
| Saxena et al. | Identification and characterization of Microbial Consortia present in sewage samples collected from Sewage Treatment Plant, Jaipur,(Raj.) | |
| Van den Berg et al. | Technical note The formulation of synthetic domestic wastewater sludge medium to study anaerobic biological treatment of acid mine drainage in the laboratory | |
| CN110317737A (en) | Compound EM microorganism formulation, preparation method and application method | |
| Komor et al. | Evaluation of denitrification rates and mechanisms in microcosm experiments with sediments and plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240301 Address after: Room 125, Building F3, Yanhe South, Dongshan District, Hegang City, Heilongjiang Province, 154100 Patentee after: Heilongjiang Suoyi Agriculture and Forestry Technology Co.,Ltd. Country or region after: China Address before: Room 601, Unit 2, Building 16, Huangtun District 3, Wuying Mountain, Tianqiao District, Jinan City, Shandong Province, 250030 Patentee before: Su Xinyue Country or region before: China |
|
| TR01 | Transfer of patent right |