Water treatment composite active microbial inoculum and application thereof
Technical Field
The invention relates to the technical field of sewage treatment, and particularly relates to a water treatment composite active microbial inoculum and application thereof.
Background
In recent years, the amount of sewage discharged has been increasing due to frequent human activities. The sewage mainly comprises domestic sewage, breeding sewage and industrial wastewater. The components of the industrial sewage are relatively complex, particularly a large amount of artificially synthesized compounds enter the environment, the substances mainly comprise ammonia nitrogen, sulfide and phosphorus-containing compounds, and due to the complexity of the structures of the substances, the traditional wastewater treatment method cannot effectively remove the pollutants; in addition, with the acceleration of the social industrialization process, a large amount of organic pollutants which are difficult to degrade, such as polycyclic aromatic hydrocarbons, halogenated compounds, polychlorinated biphenyls, organic nitrogen and phosphorus pesticides and the like, are discharged into the environment, and most of the organic pollutants are artificially synthesized, so that the types and the number of microorganisms which have high-efficiency degradation functions in natural environment are few, and the organic pollutants are in a disadvantage in interspecific competition; in addition, main pollutants in the culture water area are feed residues, excrement and some medicines, and the accumulation of nitrogen and phosphorus nutrients in the feed can cause eutrophication of the water body and can also cause the serious standard exceeding of chemical oxygen demand, ammonia nitrogen, nitrite and the like in the water body.
At present, the sewage treatment technology in China basically takes the use of chemical preparations and the combination of aeration treatment as main materials, and the chemical preparations mostly take the combination of hydrochloric acid, sodium chlorite and ammonium chlorate as main materials. The application of strong acid and strong chlorine has certain sewage treatment application effect on one hand, but brings secondary pollution of sludge along with the application of the strong acid and the strong chlorine, the aeration is reinforced by the forced feeding of gas, the problem of 'standard' is solved, but the problem of permanent cure is solved, the more pollution and more odor problem is particularly serious, the actual case of treating sewage by using a simple biological agent does not really appear on sewage treatment, and the reason is that the biological agent is not strong enough for floras. The sewage treatment by biotechnology is a development direction, but the existence of everything in the world has the functions and significance of the existence of the sewage treatment agent, so that the development of a sewage treatment microbial inoculum with strong functions and convenient application is very important.
Disclosure of Invention
The invention provides a water treatment composite active microbial inoculum with strong applicability, wide application and low production cost and application thereof in order to make up for the defects of the prior art.
The invention is realized by the following technical scheme:
a water treatment composite active microbial inoculum comprises a composite flora and an induction base which are mixed into a whole, wherein the composite flora consists of 40-55% of nitrobacteria, 10-15% of denitrifying bacteria, 5-20% of bacillus, 5-10% of pseudomonas, 8-10% of activating enzyme, 7-10% of photosynthetic bacteria and 3-5% of glycine strains by mass; the induction group consists of 5-8% of amino acid, 5-10% of yeast extract, 1-2% of chitin, 0.1-0.3 per mill of chitosan oligosaccharide and 78-90% of glucose in percentage by mass.
The invention compounds a plurality of floras with different properties, combines with an induction group to provide growth energy for the complex floras, and can lead the complex floras to spontaneously or be induced to generate a new enzyme system according to the environment, thereby having new metabolic function and degrading or converting new compounds.
The more preferable technical scheme of the invention is as follows:
the dosage of the induction group is 15-20% of the mass of the composite flora, and the induction group plays a role in assisting and inducing in the growth process of the composite flora.
The composite flora consists of 50% of nitrobacteria, 12% of denitrifying bacteria, 10% of bacillus, 8% of pseudomonas, 8% of activating enzyme, 7% of photosynthetic bacteria and 5% of glycine bacteria by mass percent, and is a preferable proportion of the composite flora.
The induction group consists of 6 mass percent of amino acid, 8 mass percent of yeast extract, 2 mass percent of chitin, 0.2 per mill of chitosan oligosaccharide and 83.98 mass percent of glucose, and is a better proportion of the induction group.
In the invention, the induction group is used as the energy and growth source of the compound flora, so that the compound flora can generate a new enzyme system by spontaneous or induced generation gradually according to the environment of the compound flora, and has a new metabolic function, thereby degrading or converting a new compound, and enabling the compound flora to have more flexible autonomy so as to adapt to different sewage treatment environments.
The nitrifying bacteria are one or more of nitrosomonas, nitrosococcus, nitrosospira, nitrobacter, nitrococcus and nitrocystis; nitrifying bacteria, as a type of aerobic bacteria, mainly comprise nitrosobacteria and nitrobacteria, and play an important role in purifying nitrogen circulating water in an aerobic environment.
The denitrifying bacteria are one or more of denitrifying bacillus, stewartia and firefly aerothrix; denitrifying bacteria, acting as heterotrophic, facultative anaerobic bacteria, can cause denitrification.
The bacillus is one or more of bacillus thuringiensis, bacillus subtilis, bacillus pumilus, bacillus anthracis and bacillus megaterium, is a strictly aerobic or facultative anaerobic bacillus with a capsule and can generate spores with special resistance to adverse conditions; the pseudomonas is one or more of pseudomonas aeruginosa, pseudomonas fluorescens, pseudomonas gangrenia and pseudomonas pseudonares, and is obligate aerobic gram-negative spore-free and capsular bacillus; the activating enzyme is Rubisco activating enzyme.
The photosynthetic bacteria are gram-negative bacteria without the capability of forming spores, can perform photosynthesis by using organic matters, sulfides, ammonia and the like in nature as hydrogen donor and carbon source under the anaerobic illumination or aerobic dark condition by using light as energy, has certain tolerance and decomposition capability on toxicants such as phenol, cyanogen and the like, and has stronger decomposition and conversion capability.
The application of the water treatment composite active microbial inoculum in sewage treatment is suitable for sewage with various pH values, various temperatures and dissolved oxygen amounts.
The composite active microbial inoculum of the invention has the functions of degrading COD, BOD, ammonia nitrogen, total nitrogen and SS, and has obvious functions of deodorizing and manuscript-repairing and eliminating NH3、P、H2The S and the organic acid have super strong capability and strong ecological restoration capability, can inhibit viruses, germs and parasites, effectively inhibit the propagation of algae and purify water and water color.
The composite active microbial inoculum of the invention also has peculiar effect on low-concentration heavy metal pollution, such as zinc, manganese, iron, chromium, cadmium, lead and arsenic.
The product of the invention has the advantages of simple preparation, low production cost, strong flora viability, easy culture, fast propagation, strong adaptability to the environment, natural evolution and other characteristics, conforms to the requirement of sewage treatment and has strong applicability.
Detailed Description
The invention is further described below with reference to specific examples to facilitate the understanding of the invention. It is apparent that the following embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: a water treatment composite active microbial inoculum comprises the following components:
composite flora (mass fraction ratio):
40% of nitrifying bacteria, 10% of denitrifying bacteria, 20% of bacillus, 5% of pseudomonas, 10% of activating enzyme, 10% of photosynthetic bacteria and 5% of glycine strain;
induction base (mass fraction):
the mass fraction is 5% of amino acid, 5% of yeast extract, 1% of chitin, 0.1% o of chitosan oligosaccharide and 88.99% of glucose;
the dosage of the induction group is 20% of the mass of the composite flora, and the two are uniformly mixed into a whole to obtain the composite active microbial inoculum.
The method is characterized in that the wastewater produced by thiophanate methyl of a certain company is taken as a treatment object, wherein the COD concentration in the wastewater is 9000mg/L, the methyl sulfide is not detected, the concentration of dimethyl disulfide is 350mg/L, and the odor is large.
Taking 100L of the wastewater, adding 100g of the composite active microbial inoculum into the wastewater, standing for 72 hours, and detecting the treated wastewater, wherein the COD concentration of the wastewater is 4500mg/L, the dimethyl sulfide concentration is 0mg/L, the dimethyl disulfide concentration is 0mg/L, and the wastewater has no foul smell; and standing for 72 hours continuously, and detecting the wastewater, wherein the COD concentration of the wastewater is 150 mg/L.
In the composite active microbial inoculum, the photosynthetic bacteria and the bacillus can convert malodorous gas hydrogen sulfide into sulfur element required by growth of the composite active microbial inoculum, thereby achieving the aim of deodorization.
Example 2: a water treatment composite active microbial inoculum comprises the following components:
composite flora (mass fraction ratio):
55% of nitrobacteria, 15% of denitrifying bacteria, 5% of bacillus, 50% of pseudomonas, 10% of activating enzyme, 7% of photosynthetic bacteria and 3% of glycine strain;
induction base (mass fraction):
the mass fraction is 8% of amino acid, 10% of yeast extract, 2% of chitin, 0.3% o of chitosan oligosaccharide and 79.97% of glucose;
the dosage of the induction group is 15 percent of the mass of the composite flora, and the induction group and the composite flora are uniformly mixed into a whole to obtain the composite active microbial inoculum.
The waste water mainly contains inorganic salts such as sodium chloride, sodium sulfate, sodium carbonate and sodium bicarbonate, wherein Na is+≤20000mg/L,SO4 2-≤30000mg/L,Cl-≤20000mg/L,CO3 2-≤4000mg/L,HCO3 -Less than or equal to 2000mg/L, and other heavy metal ions all accord with the national industrial metallurgy wastewater discharge standard.
Taking 100L of the wastewater, adding 100g of the composite active microbial inoculum into the wastewater, standing for 72 hours, and detecting the treated wastewater, wherein Na is contained in the wastewater+≤5000mg/L,SO4 2-≤5000mg/L,Cl-≤4000mg/L,CO3 2-≤200mg/L,HCO3 -Less than or equal to 100mg/L and no heavy metal ions exist.
Example 3: a water treatment composite active microbial inoculum comprises the following components:
composite flora (mass fraction ratio):
50% of nitrifying bacteria, 12% of denitrifying bacteria, 10% of bacillus, 8% of pseudomonas, 8% of activating enzyme, 7% of photosynthetic bacteria and 5% of glycine strain;
induction base (mass fraction):
the mass fraction is 6% of amino acid, 8% of yeast extract, 2% of chitin, 0.2% o of chitosan oligosaccharide and 83.98% of glucose;
the dosage of the induction group is 18 percent of the mass of the composite flora, and the two are uniformly mixed into a whole to obtain the composite active microbial inoculum.
The domestic sewage is treated by taking the domestic sewage as a treatment object, the pH value of the domestic sewage is 6-9, the total phosphorus content is 4-15mg/L, the ammonia nitrogen content is 55-100mg/L, the COD concentration is 200-400mg/L, and the BOD concentration is 100-200 mg/L.
Taking 100L of the domestic sewage, adding 100g of the composite active microbial inoculum into the sewage, standing for 72 hours, and detecting the treated sewage, wherein the total phosphorus content is 0.65mg/L, the ammonia nitrogen content is 0.8-1mg/L, the COD concentration is 50mg/L, the BOD concentration is 15mg/L, and the pH value is 7.4; and continuously standing for 72 hours, and detecting the treated sewage, wherein the total phosphorus content is 0.35mg/L, the ammonia nitrogen content is 0.05-0.1mg/L, the COD concentration is 35mg/L, the BOD concentration is 11mg/L, and the pH value is 7.2.
In the composite active microbial inoculum of the invention, the participation of the Glycerin species can become dominant bacteria in the environment, and can inhibit the growth of pathogenic bacteria and putrefying bacteria (for example, after lactobacillus and the like become dominant bacteria, the growth of Escherichia coli and the like in the environment can be inhibited, thereby reducing the generation of odor such as ammonia gas and the like).
The above-mentioned embodiments are intended to illustrate the technical solutions and advantages of the present invention, and it should be understood that the above-mentioned embodiments are only specific embodiments of the present invention, and are not intended to limit the present invention, and any modifications, additions, equivalents, etc. made within the scope of the principles of the present invention should be included in the scope of the present invention.