CN102533582A - Efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and application thereof - Google Patents
Efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and application thereof Download PDFInfo
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Abstract
The invention discloses efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and an application thereof. Aiming at the characteristics of poor biochemical perforamnce, common pollution and diverse types of PBDEs, the invention provides a kind of economical, efficient, broad-spectrum and environment-friendly PBDEs degradation bacteria and an application thereof based on the current situation of PBDEs degradation, primarily the application in degrading PBDEs in wastewater biological treatment and environment biological remediation. The efficient degradation bacteria BFR01 provided by the invention come from the soil polluted by PBDEs, and are obtained by manual enrichment and separation and purification. The strain BFR01 is gram negative bacteria which have relatively small bacterial colony, white non-transparent round shape and non-smooth surface; the thalli can be spherical or ellipsoidal with diameter of 0.5-0.7 mu m; catalase and oxidase are positive, and V-P reaction is negative; and the nitrate can be reduced, and glucose can be oxidized to generate acid. In an aerobic condition, the bacteria not only have relatively strong PBDEs degradation ability, but also can degrade PBDEs at broad spectrum, and can be applied to the PBDEs-containing wastewater biological treatment and environment biological remediation.
Description
Technical field
The invention belongs to environmental pollutant biologic treating technique field, be specifically related to a strain PBDE efficient degrading bacteria and the application in biological wastewater treatment and environmental organism reparation thereof.
Technical background
PBDE (Polybrominated diphenyl ethers; PBDEs) as a kind of inexpensive, fire retardant efficiently; Extensively added in the products such as electron device, plastics, textiles, rubber, office accommodations; Number by bromine atoms on its phenyl ring is different, and PBDEs has 209 kinds of homologs, and common in the surrounding medium have tetrabromo biphenyl ether, pentabromo-biphenyl ether, hexabromobiphenyl ether and a decabromodiphenyl oxide.Owing to there is not the effect of chemical bond between PBDEs and the product material, therefore, in the production of product, transportation, use, treating processes, the PBDEs in the product can be discharged in the environment inevitably.In atmosphere, water body, soil, animal, human body, food, all generally detect PBDEs at present, this has caused international community's showing great attention to human life safety and environmental safety.
At present; PBDEs mainly adopts chemistry, biological method to remove, wherein chemical method exist cost too high, be prone to cause deficiencies such as secondary pollution, though and biological process has advantages such as cost is low, environmental protection and energy saving; But biological process lacks broad spectrum only to decabromodiphenyl oxide at present.The PBDEs chemical method is a characteristic with catalyzed oxidation, photoxidation etc.; Like application number is 200510086490.2; Name is called the method for nanometer iron powder fast polybrominated biphenyl ether degrading, and publication number is the patent of invention of CN1739822, and it adopts nanometer iron powder degraded PBDEs; Degraded is carried out at normal temperature, and removal effect is good.And for example application number is 201010160415.7; Name is called degrade the fast method of decabromodiphenyl oxide in the organic system of nanometer Pd/C catalyzer; Publication number is the patent of invention of CN101816828A, and it is with the alcoholic solution anabolic reaction system of normal hexane or aromatic hydrocarbon, and regulates its pH to 10-11; Degraded is controlled at 50 ± 5 ℃, good degrading effect.Application number is 200810239353.1 for another example; Name is called a kind of method of surface active agent solubilization combined with UV technique degrading polybrominated diphenyl ethers using; Publication number is the patent of invention of CN101461989, and it adopts tensio-active agent to improve the solubleness of PBDEs, utilizes UV-light to make the PBDEs degraded then at normal temperatures; Whole technology weak point consuming time, efficient is high.On the other hand; Biological process utilizes different microbial inoculums; Degrading decabromodiphenyl ether is 201010019234.2 like application number, and name is called Lysinibacillus fusiformis DB-1 bacterium and the application in degrading decabromodiphenyl ether thereof; Publication number is the patent of invention of CN101864374A, and its degradation bacteria Lysinibacillus fusiformis DB-1 is degrading decabromodiphenyl ether effectively.And for example application number is 201010019235.7; Name is called a kind of subtilis and the application in degrading decabromodiphenyl ether thereof; Publication number is the patent of invention of CN101921716A, and its used subtilis (Bacillus subtilis DB-2) is degrading decabromodiphenyl ether effectively.Application number is 201010019236.1 for another example; Name is called a kind of Acinetobacter calcoaceticus and the application in degrading decabromodiphenyl ether thereof; Publication number is the patent of invention of CN101899404A, and the Acinetobacter calcoaceticus of its screening (Acinetobacter calcoaceticus DB-3) is effective degrading decabromodiphenyl ether also.Application number is 201010103021.8; Name is called a kind of restorative procedure of decabromodiphenyl oxide contaminated soil; Publication number is the patent of invention of CN101733272A, and it utilizes the Degradation of pore fungus to decabromodiphenyl oxide, thereby realizes the reparation to decabromodiphenyl oxide contaminated soil.
Pseudomonas stutzeri (Pseudomonas stutzeri) is a common indigenous microorganism in the environment such as soil, fresh water, seawater; Existing patented technology is applied to denitrogenation, dephosphorization, sulphur removal, degrading pesticide and aspects such as polycyclic aromatic hydrocarbons, antiseptic-germicide, but does not still have patented technology that this bacterium is applied to the PBDEs degraded at present.Like application number is 200910273368.4; Name is called the Pseudomonas stutzeri CY003 and the application thereof of nitrite nitrogen, nitric nitrogen and ammonia nitrogen in the efficient removal water body; Publication number is the patent of invention of CN101724594A, and its Pseudomonas stutzeri CY003 has good degradation effect to the ammonia nitrogen in the water body, nitrate nitrogen and nitrous acid nitrogen.And for example application number is 200910273046.X; Name is called the Pseudomonas stutzeri YZN-001 and the application of removing ammonia-state nitrogen, nitric nitrogen and nitrite nitrogen in the water body; Publication number is the patent of invention of CN101705202A; Under normal temperature condition, its Pseudomonas stutzeri YZN-001 is to ammonia-state nitrogen in the water, and nitric nitrogen and nitrite nitrogen have removal effect preferably.Application number is 201010538869.3 for another example; Name is called a strain Pseudomonas stutzeri and the application in water body dephosphorized thereof; Publication number is the patent of invention of CN102061275A, and its Pseudomonas stutzeri YG-26 can remove the phosphorus in the sewage to greatest extent, effectively controls body eutrophication.Application number is 03156758.4; Name is called a kind of preparation of pseudomonas stutzeri strain and to the application in the organic compounds containing sulfur desulfurization; Publication number is the patent of invention of CN1594549, and its Pseudomonas stutzeri UP-1 produces water-soluble sulfur-bearing inorganics to making the sulfurous organic compound desulfurization.Application number is 200310111556.X, and name is called Pseudomonas stutzeri ZWLR2-1 and method for making and application, and publication number is the patent of invention of CN1546647, and its Pseudomonas stutzeri ZWLR2-1 is remarkable to the chloronitrobenzene degradation effect.Application number is 200610090054.7; Name is called Pseudomonas stutzeri JSD-008 and to the Degradation of organophosphorus pesticide; Publication number is the patent of invention of CN101096644, organochlorine pesticides such as its isolating Pseudomonas stutzeri JSD-008 ability chlorpyrifos degradation and parathion-methyl.Application number is 200910303045.5, and name is called a kind of pseudomonas stutzeri strain and the application in degrading polycyclic aromatic hydrocarbon with high molecular weight thereof, and publication number is the patent of invention of CN101603022, and its bacterial strain MT-5 is better to the degradation capability of pyrene, perylene.In addition, Pseudomonas stutzeri can also be used as antiseptic-germicide.Like application number is 200910010499.3; Name is called activity extract and method for making and the purposes of a facultative anaerobic ocean Pseudomonas stutzeri; Publication number is the patent of invention of CN101496820; Its Pseudomonas stutzeri DN7 of sub-department from the ocean has bacterinertness to Pseudomonas aeruginosa and subtilis, has the latent effect as antibacterials.And for example application number is 00119018.0; Name is called pseudomonas stutzeri Zh 9944 and screening method thereof and prevents and treats the microbial preparation of bacterial wilt; Publication number is the patent of invention of CN1347981; It is the microbial preparation that main active ingredient is processed with Pseudomonas stutzeri zh9944, can effectively prevent and treat the bacterial wilt because of plants such as tobacco that Pseudomonas solanacearum causes, tomato, peanut, eggplant, capsicums.
Can find out by the foregoing invention patent; In view of the ubiquity of PDBEs pollution and the variety of PBDEs kind; Utilize that the exploitation of advantages such as the biological process cost is low, environmental protection and energy saving is a kind of to have broad spectrum, a PBDEs biological treating method that purification efficiency is high, biological wastewater treatment and environmental organism reparation are had very important realistic meaning.
Summary of the invention
The objective of the invention is poor to the PBDEs biochemical, as to pollute ubiquity and PBDEs type diversity characteristics; Degraded present situation based on PBDEs; Providing that a kind of PBDEs is economical, efficient, the PBDEs degradation bacteria and the application thereof of environmental protection, mainly is in biological wastewater treatment and environmental organism reparation, to be used to degrade the application of PBDEs.Efficient degrading bacteria BFR01 provided by the present invention derives from and receives the PBDEs Contaminated soil, obtains through artificial enrichment, separation and purification.Bacterial strain BFR01 is a Gram-negative bacteria, and its bacterium colony is less, and White-opalescent circle shape is rough; Thalli morphology is spherical or the ellipsoid shape, and diameter is 0.5-0.7 μ m, and katalase, oxydase are positive, and the V-P reaction is negative, can reduce nitrate salt and oxidizing glucose to produce sour.Through identifying that this bacterium is Pseudomonas stutzeri (Pseudomonas stutzeri), the Genbank accession number of its 16S rDNA is JF833084.Be deposited at China Committee for Culture Collection of Microorganisms common micro-organisms center (No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica) on 09 05th, 2011, its deposit number is CGMCC 5218.
The optimum growing condition of this degradation bacteria is: pH=4.0～10.0,20～50 ℃ of temperature.
This degradation bacteria can be carried out growth and breeding with PBDEs as the sole carbon source and the energy, the PBDEs in efficient degradation nutrient solution, waste water, soil and the settling.In addition, this degradation bacteria all has good degradation effect to multiple PBDEs in the pH of broad value and TR, and this degradation bacteria can tolerate the PBDEs of 1mg/L.
Notable feature of the present invention is that this degradation bacteria can the multiple bromo biphenyl of efficient degradation ether, and the degraded of PBDEs is had high efficiency and broad spectrum.
Description of drawings
The 16S rDNA gene order phylogenetic tree of Fig. 1 degradation bacteria BFR01
Embodiment
Collection in worksite receives the PBDEs Contaminated soil, and adopting with PBDEs (BDE-47 and BDE-209) is inorganic salt system domestication, the screening PBDEs efficient degrading bacteria of the sole carbon source and the energy.
The composition of inorganic salt nutrient solution (MSM): 0.086g/L CaSO
42H
2O, 0.37g/L MgSO
47H
2O, 1.7g/L K
2SO
4, 5.3g/LKH
2PO
4, 13.89g/L K
2HPO
43H
2O, 10g/LNaNO
3, 0.22mg/L FeSO
47H
2O, 1.02mg/L CoMoO
4, 1.24mg/LH
3BO
3, 3.38mg/LMnSO
4H
2O, 5mg/LCuSO
45H
2O, 5.74mg/LZnSO
47H
2O and 0.1mL/LH
2SO
4
Through taming repeatedly and ruling, obtain the pure bacterium of a strain, pure bacterium obtains PBDEs efficient degrading bacteria BFR01 at last through being seeded to its degradation capability of proof in the nutrient solution.Bacterial strain BFR01 is accredited as Pseudomonas stutzeri (Pseudomonas stutzeri) through 16S rDNA order-checking, and its phylogenetic tree is seen Fig. 1
The bacterial strain BFR01 of logarithmic phase middle and later periods is processed microbial inoculum.
Embodiment 1:
The microbial inoculum that makes is inoculated in the minimal medium that contains PBDEs (BDE-47, BDE-99, BDE-100, BDE-153, BDE-154, BDE-183, BDE-209); The concentration of PBDEs is controlled in the 20-100 μ g/L scope in the substratum; At 150r/min; 30 ℃ of following lucifuge shaking culture, after 14 days, its degradation efficiency is as shown in table 1.
The degradation efficiency of PBDEs in table 1 nutrient solution
Embodiment 2: the degraded of PBDEs in the waste water
Certain sewage work's waste water, its pH is 5.3～8.6, the kind and the concentration that contain PBDEs are as shown in table 2.The microbial inoculum (10 that adds degradation bacteria BFR01
6～10
8CFU/mL), place the room temperature lucifuge to cultivate 10 days, measure the residual quantity of PBDE, experimental result is as shown in table 2.
The degradation efficiency of PBDEs in the table 2 sewage work waste water
Embodiment 3: the degraded of PBDEs in the soil
Certain PBDEs Contaminated soil, its pH value is 4.0～10.0, kind and the content of contained PBDEs are as shown in table 3.Add the 1.0mL microbial inoculum in every gram soil, the nectar degree is controlled at 10 in the soil
6～10
8CFU/g.After 10 days, measure the wherein content of PBDEs, experimental result is as shown in table 3.
The degradation efficiency of PBDEs in table 3 soil
Embodiment 4: the degraded of PBDEs in the settling
The settling that certain PBDEs pollutes, its pH value is 6.0～9.0, kind and the content of contained PBDEs are as shown in table 4.Add the 1.0mL microbial inoculum in every gram settling, the nectar degree is controlled at 10 in the settling
6～10
8CFU/g.After 10 days, measure the content of PBDEs in the settling, experimental result is as shown in table 4.
The degradation efficiency of PBDEs in table 4 settling
Claims (2)
1. a strain PBDE degradation bacteria; It is characterized in that: this mikrobe is that the Pseudomonas stutzeri of Gram-negative belongs to the bacterial strain BFR01 of (Pseudomonas stutzeri); On September 9th, 2011 was deposited at Chinese microorganism strain preservation administrative center, and its deposit number is CGMCC 5218, and its main biological characteristics is: bacterium colony is less; White-opalescent circle shape, rough; Thalli morphology is spherical or the ellipsoid shape, and diameter is 0.5-0.7 μ m, and katalase, oxydase are positive, and the V-P reaction is negative, can reduce nitrate salt and oxidizing glucose to produce sour.The Genbank accession number of this bacterial strain 16S rDNA is JF833084.
2. the said degradation bacteria of claim 1 is characterized in that: this bacterium can be applied to purify liquid waste with environment in PBDE.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691732A (en) * | 2013-12-08 | 2014-04-02 | 浙江大学 | Method for performing phytoremediation on decabromodiphenyl ether polluted soil by alternation of wetting and drying |
| CN103740626A (en) * | 2014-01-27 | 2014-04-23 | 哈尔滨师范大学 | Bacillus laterospora with capacity of degrading BDE-47 |
| CN105969760A (en) * | 2016-06-29 | 2016-09-28 | 华南理工大学 | Embedded nano-iron/single-microorganism composite microbial agent and preparation method thereof |
| CN105969706A (en) * | 2016-07-29 | 2016-09-28 | 华南农业大学 | Acinetobacter soli for preventing and controlling bacterial wilt and application thereof |
| CN106390351A (en) * | 2016-08-19 | 2017-02-15 | 环境保护部华南环境科学研究所 | Efficient degradation method for polybrominated diphenyl ethers fire retardant |
| CN106947719A (en) * | 2017-04-19 | 2017-07-14 | 华南理工大学 | A kind of burkholderia GYP1 and its application in degraded brominated flame-retardant |
| CN111693687A (en) * | 2020-05-28 | 2020-09-22 | 长沙都正生物科技有限责任公司 | Preparation method of biological negative sample and biological standard substance and TMAO detection method |
| CN117511829A (en) * | 2024-01-05 | 2024-02-06 | 江苏聚庚科技股份有限公司 | Degradation strain and application thereof in soil remediation |
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2011
- 2011-09-20 CN CN2011102788627A patent/CN102533582A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691732A (en) * | 2013-12-08 | 2014-04-02 | 浙江大学 | Method for performing phytoremediation on decabromodiphenyl ether polluted soil by alternation of wetting and drying |
| CN103740626A (en) * | 2014-01-27 | 2014-04-23 | 哈尔滨师范大学 | Bacillus laterospora with capacity of degrading BDE-47 |
| CN105969760A (en) * | 2016-06-29 | 2016-09-28 | 华南理工大学 | Embedded nano-iron/single-microorganism composite microbial agent and preparation method thereof |
| CN105969760B (en) * | 2016-06-29 | 2020-02-18 | 华南理工大学 | Embedded nano iron/single microorganism composite microbial agent and preparation method thereof |
| CN105969706A (en) * | 2016-07-29 | 2016-09-28 | 华南农业大学 | Acinetobacter soli for preventing and controlling bacterial wilt and application thereof |
| CN105969706B (en) * | 2016-07-29 | 2019-06-28 | 华南农业大学 | For preventing and treating single acinetobacter calcoaceticus and its application of bacterial wilt |
| CN106390351A (en) * | 2016-08-19 | 2017-02-15 | 环境保护部华南环境科学研究所 | Efficient degradation method for polybrominated diphenyl ethers fire retardant |
| CN106390351B (en) * | 2016-08-19 | 2019-04-12 | 环境保护部华南环境科学研究所 | A kind of efficient degradation method of polybrominated diphenyl ethers based flame retardant |
| CN106947719A (en) * | 2017-04-19 | 2017-07-14 | 华南理工大学 | A kind of burkholderia GYP1 and its application in degraded brominated flame-retardant |
| CN111693687A (en) * | 2020-05-28 | 2020-09-22 | 长沙都正生物科技有限责任公司 | Preparation method of biological negative sample and biological standard substance and TMAO detection method |
| CN117511829A (en) * | 2024-01-05 | 2024-02-06 | 江苏聚庚科技股份有限公司 | Degradation strain and application thereof in soil remediation |
| CN117511829B (en) * | 2024-01-05 | 2024-06-11 | 江苏聚庚科技股份有限公司 | Degradation strain and application thereof in soil remediation |
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Application publication date: 20120704 |