CN110960462A

CN110960462A - Milk-beautifying nano lipid vesicle and preparation method thereof

Info

Publication number: CN110960462A
Application number: CN201911336395.1A
Authority: CN
Inventors: 孙晋喜; 刘晓微; 刘冬
Original assignee: Suzhou Greenleaf Daily Commodity Co ltd
Current assignee: Suzhou Greenleaf Daily Commodity Co ltd
Priority date: 2019-12-23
Filing date: 2019-12-23
Publication date: 2020-04-07

Abstract

The invention discloses a breast beautifying nanometer lipid vesicle and a preparation method thereof, belonging to the technical field of special cosmetics. The breast beautifying nanometer lipid vesicle comprises the following components in parts by weight: 0.10-20.00 parts of milk beautifying functional components, 1.00-10.00 parts of compound phospholipid, 0.10-5.00 parts of cholesterol, 1.00-10.00 parts of emulsifier, 2.00-20.00 parts of polyalcohol and the balance of water to 100 parts. The invention loads the milk beautifying functional components which can inhibit lipolysis, promote lipogenesis and accelerate fat cell division and proliferation so as to improve the number and volume of fat cells into the nano lipid vesicles with the particle diameter of 10-500nm, and has the characteristics of good stability, high safety (no estrogen), synergistic interaction, targeted slow release, good transdermal absorption, long retention time, high bioavailability and the like, thereby having more ideal milk beautifying effect.

Description

Milk-beautifying nano lipid vesicle and preparation method thereof

Technical Field

The invention belongs to the field of functional cosmetics, and particularly relates to a breast beautifying nano lipid vesicle and a preparation method thereof.

Background

Breasts are an important sign of sexual maturity in women, one of the most important sexual sensitive areas of women, and plump breasts can highlight the female's quality and charm. Due to the difference between diet types and genetics, the breasts of some women are more enlarged and round, the shape of the breasts is a full hemisphere or a water drop with full lower edge, and the breasts are stiff and elastic; the flat and small breast or loose and drooping of some women can not only destroy the beauty of the female curve, but also influence the physical and mental health of the female.

Women with flat breasts wish to have full breasts by breast augmentation in order to enhance personal feminine appeal. Common breast enlargement methods include diet breast enlargement, sport breast enlargement, acupuncture breast enlargement, essential oil breast enlargement, medicine breast enlargement, operation breast enlargement, and the like. The above methods have little effect, high economic cost and great risk, which makes some women who love beauty feel more annoying or disappointed.

With the continuous development of the cosmetic market in China, breast-enlarging cosmetics gradually appear on the market. Because of the low relative cost and convenient use, the breast augmentation cosmetics are popular with some women. The desire for beauty is risky and confusing to consumers is the mixing of these breast augmentation cosmetics, fish and dragon, and some businessly people add drugs, estrogens, etc. to breast augmentation products. While some effectiveness may be obtained with such breast augmentation cosmetics, prolonged use may result in endocrine disorders, increased risk of or breast disease.

The breast is mainly composed of glands, ducts, adipose tissue, fibrous tissue, and the like. The fat tissue in the breast is wrapped around the mammary gland in a saccular manner to form a hemispherical whole, the amount of the saccular fat tissue is one of important factors for determining the size of the breast, and the individual difference can be caused by factors such as genes, diet, age and the like.

Theoretically, the amount and volume of adipocytes can be increased by the transdermal absorption of pharmaceutical and cosmetic raw materials that can increase mammary cystic adipose tissue. However, it is difficult for these drugs or cosmetic materials to actually pass through the thick epidermis or dermis due to the barrier action of the skin, and act on the underlying adipose tissue.

The traditional breast enlargement cosmetics are prepared by adding some functional plant extracts into a micron emulsification system or water, so that the activity of the plant extracts is easily lost due to the change of external environment (illumination, oxygen, temperature, pH value and the like), and even if the stable functional plant extracts are difficult to penetrate through thick epidermis and dermis to act on the fat tissue below the thick epidermis and the dermis, the due breast beautifying effect is difficult to achieve.

Therefore, the improvement of the safety, stability and permeability of the breast beautifying functional components is a difficult problem to be solved urgently for realizing the efficacy of the breast beautifying functional cosmetics.

Disclosure of Invention

The purpose of the invention is as follows: the invention aims to make up the defects of the existing breast beautifying cosmetic research technology, provides a breast beautifying nano lipid vesicle, can encapsulate water-soluble and oil-soluble functional components with the functions of inhibiting lipolysis, promoting lipogenesis and accelerating fat cell division and proliferation in an interlayer of a hydrophilic or hydrophobic group of the nano lipid vesicle, and has the characteristics of good stability, high safety (no estrogen), synergistic interaction, targeted slow release, good transdermal absorption, long retention time, high bioavailability and the like, thereby having ideal breast beautifying effect.

The technical scheme is as follows: a nano-lipid vesicle for beautifying milk contains the following components in percentage by weight: 0.10-20.00 parts of breast beautifying functional components, 1.00-10.00 parts of compound phospholipid, 0.10-5.00 parts of sterol, 1.00-10.00 parts of emulsifier, 2.00-20.00 parts of polyalcohol and the balance of water.

Preferably, the milk beautifying functional component comprises a functional component having the functions of inhibiting lipolysis, promoting lipogenesis, accelerating adipocyte division and proliferation, thereby increasing the number and volume of adipocytes, can be encapsulated in the interlayer of hydrophilic or hydrophobic groups of the nano lipid vesicle, and is at least one selected from the group consisting of ornithine, commiphora mukul resin extract, rhizoma anemarrhenae root extract, garcinia mangostana peel extract, africana tree fruit extract, quince seed extract, quillaja bark extract, paeonia radix extract, chlorella/lupin protein fermentation product.

Preferably, the complex phospholipid comprises synthetic and natural sources and is selected from at least one of lecithin, soybean lecithin, lysolecithin, hydrogenated lecithin, hydrogenated lysolecithin, hydroxylated lecithin, phosphatidylcholine, glycerophosphorylcholine and hydrogenated phosphatidylcholine.

Preferably, the sterols include animal and plant origin selected from the group consisting of cholesterol, 7-dehydrocholesterol, and mixtures thereof,

β -sitosterol or dihydrocholesterol.

Preferably, the emulsifier is a nonionic surfactant, and is at least one selected from the group consisting of polyglyceryl-10 pentastearate, PEG-100 stearate, C12-20 alkyl glucoside, PEG-20 behenyl alcohol, methyl glucose sesquiisostearate, PEG-20 methyl glucose sesquistearate, glyceryl stearate, PEG-75 stearate, PEG-120 methyl glucose dioleate, ceteth-20, steareth-20, polysorbate-20, polyglyceryl-10 myristate, decyl glucoside, and sorbitan olive oleate.

Preferably, the polyhydric alcohol is at least one selected from the group consisting of glycerin, ethanol, propylene glycol, 1, 3-propanediol, butylene glycol, 1, 2-pentanediol, hexylene glycol, octylene glycol, dipropylene glycol, 1, 2-hexanediol, and 1, 2-butanediol.

The invention also discloses a preparation method of the breast beautifying nano lipid vesicle, which comprises the following steps:

1) mixing the composite phospholipid, the sterol and the emulsifier, stirring and heating until the solid material is completely melted to obtain an oil phase mixture;

2) mixing polyalcohol and water, heating and stirring uniformly to obtain a water phase mixture;

3) adding the oil phase mixture obtained in the step 1) into the water phase mixture obtained in the step 2), adding a milk beautifying functional component, and homogenizing at a high speed to obtain a micron emulsified body;

4) and (3) carrying out multiple high-pressure (300-1200bar) homogenization or high-speed microjet treatment on the micron emulsified body obtained in the step 3) to obtain a nanometer emulsified body with the particle size of 10-500 nm.

The third purpose of the invention is to disclose the application of the milk beautifying nanometer lipid vesicle in cosmetics.

The invention has the beneficial effects that:

the nano-sized breast beautifying lipid vesicle encapsulates water-soluble and oil-soluble functional components with the functions of inhibiting lipolysis, promoting lipogenesis and accelerating fat cell division and proliferation in the interlayer of hydrophilic or hydrophobic groups of the nano-sized lipid vesicle with the particle diameter of 10-500nm, and has the characteristics of good stability, high safety (no estrogen), synergistic interaction, targeted sustained release, good transdermal absorption, long retention time, high bioavailability and the like, thereby having ideal breast beautifying effect.

Drawings

Fig. 1 is a schematic diagram of the rate of change of breast volume.

Detailed Description

The present invention will be described below with reference to specific examples, but the present invention is not limited thereto. The materials described in the following examples are commercially available without specific reference, and the following examples are not intended to limit the scope of the present invention, and all equivalent implementations or modifications without departing from the scope of the present invention are intended to be encompassed by the present claims.

The preparation method comprises the following steps:

Example 1

The breast beautifying nanometer lipid vesicle of the embodiment comprises the following components in parts by weight:

the functional components of the breast beautifying cream are as follows: 0.55 part of ornithine, 1.00 part of commiphora mukul resin extract, 2.00 parts of rhizoma anemarrhenae root extract, 5.00 parts of mangosteen pericarp extract and 5.00 parts of african pendent lamp tree extract; and (3) composite phospholipid: 3.00 parts of hydrogenated lecithin and 4.00 parts of lecithin; sterol: 3.00 parts of cholesterol and 2.00 parts of dihydrocholesterol; emulsifier: 2.00 parts of polyglycerol-10 myristate and 3.00 parts of PEG-20 tribehenate; polyol: 10.00 parts of butanediol and 3.00 parts of 1, 2-pentanediol; 59.45 parts of water.

Example 2

the functional components of the breast beautifying cream are as follows: 5.00 parts of mangosteen pericarp extract, 10.00 parts of african dropwort tree fruit extract and 5.00 parts of quince seed extract; and (3) composite phospholipid: 6.00 parts of hydrogenated lecithin and 4.00 parts of hydrogenated phosphatidylcholine; sterol: 0.10 part of 7-dehydrocholesterol; emulsifier: 1.00 part of PEG-75 stearate; polyol: 2.00 parts of 1, 2-hexanediol; 66.90 parts of water.

Example 3

the functional components of the breast beautifying cream are as follows: ornithine 0.05 part, peony root extract 0.05 part; and (3) composite phospholipid: 1.00 part of hydrogenated lecithin; sterol: 5.00 parts of cholesterol; emulsifier: 7.00 parts of C12-20 alkyl glucoside, 3.00 parts of PEG-20 methyl glucose sesquistearate; polyol: 15.00 parts of ethanol and 5.00 parts of propylene glycol; 63.90 parts of water.

Comparative example 1

The nano lipid vesicle of the comparative example consists of the following components in parts by weight:

and (3) composite phospholipid: 3.00 parts of hydrogenated lecithin and 4.00 parts of lecithin; sterol: 3.00 parts of cholesterol and 2.00 parts of dihydrocholesterol; emulsifier: 2.00 parts of polyglycerol-10 myristate and 3.00 parts of PEG-20 tribehenate; polyol: 10.00 parts of butanediol and 3.00 parts of 1, 2-pentanediol; 59.45 parts of water.

In contrast to example 1, comparative example 1 did not contain any milk efficacy ingredient.

Comparative example 2

The breast beautifying micron emulsion of the comparative example consists of the following components in parts by weight:

Compared with the example 1, the micron emulsified body obtained by the comparative example 2 after high-speed homogenization is not subjected to multiple times of high-pressure homogenization or high-speed microjet treatment.

The bodies prepared in examples 1 to 3 and comparative examples 1 to 2 were subjected to performance evaluations, which included: 1. stability test

The shapes, particle diameters, and PDI (polydispersity index) of the materials prepared in examples 1 to 3 and comparative examples 1 to 2 were examined at 0 day, 30 days, 60 days, and 90 days, respectively, after placing the materials in a closed container at room temperature for 3 months, and the specific test results are shown in table 1.

As can be seen from Table 1, the nano-lipid vesicles (examples 1-3 and comparative example 1) prepared according to the invention have good stability, the particle size is still distributed between 10-100nm even after being placed for 90 days, the PDI is less than 0.4, and the abnormal phenomena such as agglomeration, delamination and the like do not occur, thus meeting the requirements of practical application.

2. Safety test

Keratinocyte (HaCat cell) and skin fibroblast (HSF cell) are respectively treated by trypsin solution, diluted milk beautifying nanometer lipid vesicles prepared in examples 1-3 are respectively added, OD values are respectively tested at the wavelength of 450nm after 24 hours, and the survival rates of the HaCat cell and the HSF cell are respectively calculated, and the specific results are shown in Table 2.

As can be seen from table 2, the nano-sized milk lipid vesicles prepared according to the present invention, after being diluted 30-120 times, have no toxicity to HaCat cells and HSF cells (the survival rates of the cells are not significantly different from those of the blank control and are all close to 100.00%), and the complete morphology of the HaCat cells and the HSF cells can be observed under an electron microscope, which indicates that the nano-sized milk lipid vesicles prepared according to the present invention do not stimulate the skin.

3. Adipogenesis promotion test

Human adipocytes were cultured with 10-fold diluted milk-beautifying nano-lipid vesicles prepared in examples 1-3 at 37 ℃ for 5 hours, respectively, and then the change in fat content was measured, and the specific results are shown in Table 3.

As can be seen from Table 3, the nano-sized lipid vesicles for breast beauty prepared according to the invention can effectively promote lipogenesis after being diluted by 10 times.

4. Breast volume augmentation efficacy test

Young healthy women, 30 with 22-35 years old, 30-30A breast cups, were randomly selected into 2 groups. Wherein a group of women applied the bodies prepared in example 1 and comparative example 1 to the left and right breasts twice a day in the morning and evening, respectively; another group of women applied the bodies prepared in example 1 and comparative example 2 to the left and right breasts, respectively, twice a day in the morning and evening. The volume change of the bilateral breasts was measured by PRIMOS volume imager at 0, 60 and 90 days of continuous use, respectively, and the results are shown in fig. 1.

As can be seen from fig. 1, the increased volume of the female breast using the milk beautifying nanoliposome prepared in example 1 is significantly greater than the increased volume of comparative example 1 without any milk beautifying functional ingredient and comparative example 2 without nano treatment, which indicates that the long-term use of the milk beautifying nanoliposome prepared in the present invention can effectively increase the volume of the female breast.

It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explaining the principles of the invention and are not to be construed as limiting the invention. Therefore, any modification, equivalent replacement, improvement and the like made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. Further, it is intended that the appended claims cover all such variations and modifications as fall within the scope and boundaries of the appended claims or the equivalents of such scope and boundaries.

Claims

1. The breast beautifying nanometer lipid vesicle is characterized by comprising the following components in parts by weight: 0.10-20.00 parts of breast beautifying functional components, 1.00-10.00 parts of compound phospholipid, 0.10-5.00 parts of sterol, 1.00-10.00 parts of emulsifier, 2.00-20.00 parts of polyalcohol and the balance of water to 100 parts.

2. The milk-beautifying nanoliposome according to claim 1, wherein the milk-beautifying effective component is at least one selected from ornithine, commiphora mukul resin extract, rhizoma anemarrhenae root extract, garcinia mangostana pericarp extract, african pendent tree fruit extract, quince seed extract, quillaja bark extract, peony root extract, chlorella/lupin protein fermentation product.

3. The milk beautifying nanoliposome of claim 1, wherein the complex phospholipid comprises synthetic and natural sources selected from at least one of lecithin, soy lecithin, lysolecithin, hydrogenated lecithin, hydrogenated lysolecithin, hydroxylated lecithin, phosphatidylcholine, glycerophosphocholine, hydrogenated phosphatidylcholine.

4. The nanoemulsion vesicle according to claim 1, wherein the sterol comprises animal and plant origin and is selected from at least one of cholesterol, 7-dehydrocholesterol, β -sitosterol, dihydrocholesterol.

5. The milk beautifying nanoliposome according to claim 1, wherein the emulsifier is a nonionic surfactant selected from at least one of polyglyceryl-10 pentastearate, PEG-100 stearate, C12-20 alkyl glucoside, PEG-20 behenyl PEG-20 esters, methyl glucose sesquiisostearate, PEG-20 methyl glucose sesquistearate, glyceryl stearate, PEG-75 stearate, PEG-120 methyl glucose dioleate, ceteth-20, steareth-20, polysorbate-20, polyglyceryl-10 myristate, decyl glucoside, sorbitan olivetoleate.

6. The milk beautifying nanoliposome according to claim 1, wherein the polyhydric alcohol is at least one selected from glycerol, ethanol, propylene glycol, 1, 3-propanediol, butylene glycol, 1, 2-pentanediol, hexylene glycol, caprylyl glycol, dipropylene glycol, 1, 2-hexanediol, and 1, 2-butanediol.

7. A preparation method of the breast beautifying nanometer lipid vesicle according to claims 2-6, characterized by comprising the following steps:

3) adding the oil phase mixture obtained in the step 1) into the water phase mixture obtained in the step 2), adding the functional components for beautifying the milk, and homogenizing at a high speed to obtain a micron emulsified body;

4) and (3) carrying out multiple high-pressure, 300-1200bar homogenization or high-speed microjet treatment on the micron emulsified body obtained in the step 3) to obtain the nanometer emulsified body with the particle size of 10-500 nm.

8. Use of the milk beautifying nanoliposome vesicle according to any one of claims 1-6 in cosmetics.