CN110892938A - Method for preparing biological feed by using filter mud of sugar refinery and application thereof - Google Patents

Method for preparing biological feed by using filter mud of sugar refinery and application thereof Download PDF

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Publication number
CN110892938A
CN110892938A CN201911142497.XA CN201911142497A CN110892938A CN 110892938 A CN110892938 A CN 110892938A CN 201911142497 A CN201911142497 A CN 201911142497A CN 110892938 A CN110892938 A CN 110892938A
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fermentation
biological feed
filter mud
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parts
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张宝龙
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LIUZHOU JINGYUAN BIOLOGICAL SCIENCE & TECHNOLOGY Co Ltd
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LIUZHOU JINGYUAN BIOLOGICAL SCIENCE & TECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
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  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
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  • Birds (AREA)
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  • Marine Sciences & Fisheries (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention provides a method for preparing biological feed by using filter mud of a sugar refinery, which comprises the following steps: 1) mixing the sugar mill filter mud, rice bran and the fermentation compound uniformly, and fermenting for 20-25 days to obtain a primary fermentation material; 2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10-12 days to obtain the biological feed. The invention has the characteristics of resource utilization of sugar refinery filter mud, promotion of growth of cultured organisms, increase of dissolved oxygen in culture water, and the like.

Description

Method for preparing biological feed by using filter mud of sugar refinery and application thereof
Technical Field
The invention relates to the field of biological feed. More specifically, the invention relates to a method for preparing biological feed by using filter mud of a sugar refinery.
Background
The filter mud of the sugar refinery is waste generated in the production of cane sugar in the sugar industry, and the filter mud also contains various organic matters inside. If the fertilizer is directly put into the environment, the problems of resource waste, environmental pollution and the like exist. The sugar mill filter mud is used for preparing the aquaculture biological feed, and the reutilization of the sugar mill filter mud is realized, however, the feed prepared by the sugar mill filter mud in the prior art only can play a role of feeding aquaculture organisms, and has no regulating effect on the water quality of aquaculture water.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
Still another object of the present invention is to provide a method for preparing a biological feed using sugar mill sludge, which can utilize sugar mill sludge as a resource and increase dissolved oxygen in aquaculture water when used in aquaculture.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for preparing a biological feed using sugar mill sludge, comprising the steps of:
1) mixing the sugar mill filter mud, rice bran and the fermentation compound uniformly, and fermenting for 20-25 days to obtain a primary fermentation material;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10-12 days to obtain the biological feed.
Preferably, in the step 1), the sugar mill filter mud and the rice bran are uniformly mixed according to the mass ratio of 8-9:1, and 2-3 kg of fermentation compound is added to each ton of mixture for fermentation.
Preferably, the fermentation compound comprises fermentation bacteria and compound protease, wherein the fermentation bacteria comprise bacillus subtilis, photosynthetic bacteria, saccharomycetes, lactic acid bacteria, pseudomonas aurantiaca, aspergillus oryzae, aspergillus niger and gibberella barnacle; the compound protease comprises a solarizing enzyme and a proteolytic enzyme. The gibberellic disease and the pseudomonas aurantiaca are added, and the growth of the photosynthetic bacteria is promoted by utilizing the phytohormone secreted by the trichoderma aurantiaca and the pseudomonas aurantiaca, so that the photosynthetic bacteria can grow and propagate quickly after the prepared biological feed is put into a water body, and the dissolved oxygen of the culture water body is improved quickly; the Pseudomonas aurantiaca has the function of secreting plant hormone indoleacetic acid, and the growth of photosynthetic bacteria is promoted by adding the indoleacetic acid secreted by the Pseudomonas aurantiaca; the bacillus subtilis, the aspergillus oryzae and the aspergillus niger can degrade proteins in the soybean meal into active peptides with low molecular weight, and the absorption and utilization of nutrients by aquaculture organisms are promoted.
Preferably, the preparation method of the fermentation compound comprises the following steps: adding 100 parts by weight of distilled water into 50-60 parts by weight of humus soil, stirring for 10-20min, filtering, collecting filtrate, adding 30-35 parts by weight of rice bran, 20-25 parts by weight of soybean meal, 10-15 parts by weight of corn flour, 5-10 parts by weight of citric acid, 3-5 parts by weight of tartronic acid and 0.1-0.5 part by weight of zymophyte into 50-60 parts by weight of filtrate, fermenting at room temperature for 48-60 hours, and turning over the materials every 10-12 hours during the fermentation process to obtain the fermentation compound. The humus contains rich organic matters and mineral substances, and the leachate of the humus is mixed with rice bran, soybean meal and the like, so that the nutrient substances in the prepared fermented product are balanced and close to the natural growth environment, and microorganisms in the prepared biological feed have strong adaptability.
Preferably, the mass ratio of the bacillus subtilis, the photosynthetic bacteria, the yeast, the lactic acid bacteria, the pseudomonas aurantiaca, the aspergillus oryzae, the aspergillus niger and the gibberella bardawil is 10-15:20-30:5-10:6-12:3-5:1-3:1-3: 0.5-1.
Preferably, the fresh plant material is one or more of alfalfa, fresh sugarcane tails, sweet potato processing leftovers or horseshoe processing leftovers.
Preferably, the plant fresh materials also comprise mistletoe, sweet wormwood, glossy privet fruit, liquorice and dried orange peel. Mistletoe, herba Artemisiae Annuae, fructus Ligustri Lucidi, Glycyrrhrizae radix and pericarpium Citri Tangerinae, and has effects of promoting growth of photosynthetic bacteria and yeast.
An application of biological feed prepared from sugar mill filter mud in breeding aquatic animals, livestock and poultry is provided.
The invention at least comprises the following beneficial effects: the fermentation compound is used for fermenting the sugar mill filter mud and the rice bran so as to decompose macromolecular substances into small molecular substances, and the palatability of the sugar mill filter mud is improved in the fermentation process, so that fish prefers to eat; the corn flour, the soybean flour and the fresh plant materials are added into the primary fermentation material, and then the fermentation compound is added again for secondary fermentation, so that macromolecular fibrous substances are further decomposed under the action of bacteria, and the utilization rate of filter mud and rice bran of a sugar refinery is improved; by adding the fresh plant materials for fermentation, the fresh taste of the feed is increased, the food calling effect of the feed is enhanced, and the effect of supplementing vitamins of the feed is achieved; the plant growth hormone has the function of promoting the growth of photosynthetic bacteria, so that the growth of the photosynthetic bacteria is promoted by adding the gibberellic disease, the pseudomonas aurantiaca and the plant hormone secreted by the pseudomonas aurantiaca, the photosynthetic bacteria can quickly grow and reproduce after the prepared biological feed is put into a water body, and the dissolved oxygen of the culture water body is improved; the soybean meal contains rich grease and protein, the addition of the soybean meal into the feed can not only play a role in increasing the protein in the feed, but also promote the synthesis and secretion of gibberellin by the gibberella barnachosa so as to promote the growth of photosynthetic bacteria; the Pseudomonas aurantiaca has the function of secreting plant hormone indoleacetic acid, and the growth of photosynthetic bacteria is promoted by adding the indoleacetic acid secreted by the Pseudomonas aurantiaca; the bacillus subtilis, the aspergillus oryzae and the aspergillus niger can degrade proteins in the soybean meal into active peptides with low molecular weight, and the absorption and utilization of nutrients by aquaculture organisms are promoted. The invention has the characteristics of resource utilization of sugar refinery filter mud, promotion of growth of cultured organisms, increase of dissolved oxygen in culture water, and the like.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
A method for preparing biological feed by using filter mud of a sugar refinery comprises the following steps:
1) mixing the sugar mill filter mud, rice bran and the fermentation compound uniformly, and fermenting for 20 days to obtain a primary fermentation material;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10 days to obtain the biological feed.
Example 2
A method for preparing biological feed by using filter mud of a sugar refinery comprises the following steps:
1) uniformly mixing the filter mud of the sugar refinery and the rice bran according to the mass ratio of 9:1, and fermenting for 20 days after 3 kg of fermentation compound is added into each ton of mixture to obtain a primary fermentation material;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 12 days to obtain the biological feed.
Example 3
A method for preparing biological feed by using filter mud of a sugar refinery comprises the following steps:
1) uniformly mixing the sugar mill filter mud and the rice bran according to the mass ratio of 8:1, adding 2-3 kg of fermentation compound into each ton of mixture, and fermenting for 25 days to obtain a primary fermentation material; wherein the fermentation compound comprises fermentation bacteria and compound protease, and the fermentation bacteria comprise bacillus subtilis, photosynthetic bacteria, saccharomycetes, lactic acid bacteria, pseudomonas aurantiaca, aspergillus oryzae, aspergillus niger and gibberella barnacle; the compound protease comprises a solarizing enzyme and a protein hydrolase;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10 days to obtain the biological feed.
The gibberellin gambieri and the pseudomonas aurantiaca are added, and the growth of photosynthetic bacteria is promoted by utilizing the phytohormones secreted by the gibberellin gambieri and the pseudomonas aurantiaca, so that the prepared biological feed can rapidly grow and propagate after being put into a water body, and the dissolved oxygen of the culture water body is improved; the Pseudomonas aurantiaca has the function of secreting plant hormone indoleacetic acid, and the growth of photosynthetic bacteria is promoted by adding the indoleacetic acid secreted by the Pseudomonas aurantiaca; the bacillus subtilis, the aspergillus oryzae and the aspergillus niger can degrade proteins in the soybean meal into active peptides with low molecular weight, and the absorption and utilization of nutrients by aquaculture organisms are promoted.
Example 4
A method for preparing biological feed by using filter mud of a sugar refinery comprises the following steps:
1) uniformly mixing the sugar mill filter mud and the rice bran according to the mass ratio of 8:1, adding 3 kg of fermentation compound into each ton of mixture, and fermenting for 20 days to obtain a primary fermentation material; wherein the fermentation compound comprises fermentation bacteria and compound protease, and the fermentation bacteria comprise bacillus subtilis, photosynthetic bacteria, saccharomycetes, lactic acid bacteria, pseudomonas aurantiaca, aspergillus oryzae, aspergillus niger and gibberella barnacle; the compound protease comprises a solarizing enzyme and a protein hydrolase; the preparation method of the fermentation compound comprises the following steps: adding 100 kg of distilled water into 50 parts by weight of humus (the water content is 40%), stirring for 10min, filtering, collecting filtrate, adding 30 kg of rice bran, 20 kg of soybean meal, 10 kg of corn flour, 10 kg of citric acid, 5 kg of tartronic acid and 0.1 kg of zymocyte into 50 kg of filtrate, fermenting for 48 hours at room temperature, and turning over materials every 10 hours in the fermentation process to obtain the fermentation compound;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10 days to obtain the biological feed.
The humus contains rich organic matters and mineral substances, and the leachate of the humus is mixed with rice bran, soybean meal and the like, so that the nutrient substances in the prepared fermented product are balanced and close to the natural growth environment, and microorganisms in the prepared biological feed have strong adaptability.
Example 5
A method for preparing biological feed by using filter mud of a sugar refinery comprises the following steps:
1) uniformly mixing the sugar mill filter mud and the rice bran according to the mass ratio of 8:1, adding 2 kg of fermentation compound into each ton of mixture, and fermenting for 25 days to obtain a primary fermentation material; wherein the fermentation compound comprises zymophyte and compound protease; the fermentation bacteria comprise bacillus subtilis, photosynthetic bacteria, saccharomycetes, lactic acid bacteria, pseudomonas aurantiaca, aspergillus oryzae, aspergillus niger and gibberella barnachos in a mass ratio of 10:20:5:6:3:1:1: 0.5; the preparation method of the fermentation compound comprises the following steps: adding 100 kg of distilled water into 60 kg of humus (the water content is 30%), stirring for 20min, filtering, collecting filtrate, adding 35 kg of rice bran, 25 kg of soybean meal, 15 kg of corn flour, 10 kg of citric acid, 5 kg of tartronic acid and 0.1 kg of zymophyte into 60 kg of filtrate, fermenting for 60 hours at room temperature, and turning over materials every 12 hours in the fermentation process to obtain the fermentation compound;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10 days to obtain the biological feed.
Example 6
A method for preparing biological feed by using filter mud of a sugar refinery comprises the following steps:
1) uniformly mixing the sugar mill filter mud and the rice bran according to the mass ratio of 8:1, adding 3 kg of fermentation compound into each ton of mixture, and fermenting for 20 days to obtain a primary fermentation material; wherein the fermentation compound comprises zymophyte and compound protease; the fermentation bacteria comprise bacillus subtilis, photosynthetic bacteria, saccharomycetes, lactic acid bacteria, pseudomonas aurantiaca, aspergillus oryzae, aspergillus niger and gibberella barnachos in a mass ratio of 15:30:10:12:5:3:3: 1; the compound protease comprises a solarizing enzyme and a protein hydrolase; the preparation method of the fermentation compound comprises the following steps: adding 100 kg of distilled water into 60 kg of humus (the water content is 35%), stirring for 10min, filtering, collecting filtrate, adding 35 kg of rice bran, 20 kg of soybean meal, 15 kg of corn flour, 10 kg of citric acid, 5 kg of tartronic acid and 0.5 kg of zymophyte into 60 kg of filtrate, fermenting for 60 hours at room temperature, and turning over the materials every 10-12 hours during the fermentation process to obtain the fermentation compound;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10 days to obtain the biological feed; wherein the fresh plant material is fresh sugarcane tails.
Example 7
A method for preparing biological feed by using filter mud of a sugar refinery comprises the following steps:
1) uniformly mixing the sugar mill filter mud and the rice bran according to the mass ratio of 8:1, adding 3 kg of fermentation compound into each ton of mixture, and fermenting for 20 days to obtain a primary fermentation material; wherein the fermentation compound comprises zymophyte and compound protease; the fermentation bacteria comprise bacillus subtilis, photosynthetic bacteria, saccharomycetes, lactic acid bacteria, pseudomonas aurantiaca, aspergillus oryzae, aspergillus niger and gibberella barnachos in a mass ratio of 10:20:5:12:5:3:1: 1; the compound protease comprises a solarizing enzyme and a protein hydrolase; the preparation method of the fermentation compound comprises the following steps: adding 100 kg of distilled water into 50 kg of humus (the water content is 40%), stirring for 10min, filtering, collecting filtrate, adding 30 kg of rice bran, 25 kg of soybean meal, 15 kg of corn flour, 10 kg of citric acid, 3 kg of tartronic acid and 0.3 kg of zymophyte into 60 kg of filtrate, fermenting for 60 hours at room temperature, and turning over materials every 10 hours in the fermentation process to obtain the fermentation compound;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10 days to obtain the biological feed; wherein the fresh plant materials are alfalfa, mistletoe, sweet wormwood, glossy privet fruit, liquorice and dried orange peel.
Mistletoe, herba Artemisiae Annuae, fructus Ligustri Lucidi, Glycyrrhrizae radix and pericarpium Citri Tangerinae, and has effects of promoting growth of photosynthetic bacteria and yeast.
Effect test 1
Grass carp fries of the same size were fed with commercial grass carp feeds and the biological feeds prepared in examples 1 to 7, respectively, for a feeding period of 3 months, and the average body weight of grass carps was weighed and calculated, and the results are shown in table 1.
TABLE 1
Fish weight (g)
Example 1 1513
Example 2 1559
Example 3 1599
Example 4 1601
Example 5 1633
Example 6 1620
Example 7 1599
Commercial feed 638
As can be seen from the results in Table 1, the biological feed prepared by the invention has the function of remarkably promoting the growth of grass carps.
Effect test 2
In the morning of sunny weather, 300 kilograms of the biological feed prepared by the method is put in each mu of fishpond, oxygen increasing equipment is not used, the content of dissolved oxygen, ammonia nitrogen and nitrite in water is detected after 24 hours, and the result is shown in table 2.
TABLE 2
Dissolved oxygen (mg/L) Ammonia nitrogen (mg/L) Nitrite (mg/L)
Example 1 6.3 2.1 0.01
Example 2 6.6 2.3 0.01
Example 3 6.2 1.9 0.01
Example 4 6.4 1.9 0.01
Example 5 6.6 2.1 0.01
Example 6 6.7 1.8 0.01
Example 7 7.3 1.4 0.01
Before feeding 3.6 3.8 0.16
As can be seen from the results in Table 2, the biological feed prepared by feeding the feed disclosed by the invention has the effects of reducing the contents of ammonia nitrogen and nitrite, and the nitrite content is lower than 0.01mg/L after 24 hours, so that the effect is obvious. And the dissolved oxygen content in the aquaculture water is obviously improved by more than 1 time, and the oxygenation effect is obvious. When the feed is fed, an aerator is used for 5 hours every day, and the dissolved oxygen in the water body reaches over 10 mg/L.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the uses set forth in the specification and examples. It can be applied to all kinds of fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. The invention is therefore not to be limited to the specific details described herein, without departing from the general concept as defined by the appended claims and their equivalents.

Claims (8)

1. A method for preparing biological feed by using filter mud of a sugar refinery is characterized by comprising the following steps:
1) mixing the sugar mill filter mud, rice bran and the fermentation compound uniformly, and fermenting for 20-25 days to obtain a primary fermentation material;
2) adding corn flour, soybean flour, fresh plant materials and a fermentation compound into the primary fermentation material, and fermenting for 10-12 days to obtain the biological feed.
2. The method for preparing a biological feed by using the sugar mill filter mud as claimed in claim 1, wherein in the step 1), the sugar mill filter mud and the rice bran are uniformly mixed according to the mass ratio of 8-9:1, and 2-3 kg of fermentation compound is added to each ton of the mixture for fermentation.
3. The method of claim 1, wherein the fermentation complex comprises fermentation bacteria and complex protease, the fermentation bacteria comprises bacillus subtilis, photosynthetic bacteria, yeast, lactic acid bacteria, pseudomonas aurantiaca, aspergillus oryzae, aspergillus niger and gibberella barnachos; the compound protease comprises a solarizing enzyme and a proteolytic enzyme.
4. The method for preparing a biological feed using sugar mill sludge as claimed in claim 3, wherein the preparation method of the fermentation complex is: adding 100 parts by weight of distilled water into 50-60 parts by weight of humus soil, stirring for 10-20min, filtering, collecting filtrate, adding 30-35 parts by weight of rice bran, 20-25 parts by weight of soybean meal, 10-15 parts by weight of corn flour, 5-10 parts by weight of citric acid, 3-5 parts by weight of tartronic acid and 0.1-0.5 part by weight of zymophyte into 50-60 parts by weight of filtrate, fermenting at room temperature for 48-60 hours, and turning over the materials every 10-12 hours during the fermentation process to obtain the fermentation compound.
5. The method for preparing a biological feed by using sugar mill sludge according to claim 3, wherein the mass ratio of the bacillus subtilis, the photosynthetic bacteria, the yeast, the lactic acid bacteria, the pseudomonas aurantiaca, the aspergillus oryzae, the aspergillus niger and the gibberella barnaci is 10-15:20-30:5-10:6-12:3-5:1-3:1-3: 0.5-1.
6. The method for preparing biological feed by using filter mud of a sugar refinery according to claim 1, wherein the fresh plant material is one or more of alfalfa, fresh sugarcane tails, sweet potato processing leftovers or horseshoe processing leftovers.
7. The method of claim 6, wherein the fresh plant material further comprises mistletoe, Artemisia annua, Ligustrum lucidum, Glycyrrhiza uralensis and Citrus reticulata.
8. An application of biological feed prepared by using filter mud of a sugar refinery is characterized in that the biological feed is used for feeding aquatic livestock, livestock and poultry.
CN201911142497.XA 2019-11-20 2019-11-20 Method for preparing biological feed by using filter mud of sugar refinery and application thereof Pending CN110892938A (en)

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Cited By (3)

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CN112456653A (en) * 2020-10-27 2021-03-09 广西诺亚生物科技发展有限公司 Compound for adjusting aquaculture water quality and preparation process thereof
CN112535245A (en) * 2020-11-20 2021-03-23 广西大学 Fish feed and production method thereof
CN112841399A (en) * 2021-01-25 2021-05-28 柳州市绿威生物科技有限公司 Fermentation composition for sugar-making filter mud, preparation method thereof and treatment process for sugar-making filter mud

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CN112535245A (en) * 2020-11-20 2021-03-23 广西大学 Fish feed and production method thereof
CN112841399A (en) * 2021-01-25 2021-05-28 柳州市绿威生物科技有限公司 Fermentation composition for sugar-making filter mud, preparation method thereof and treatment process for sugar-making filter mud

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Application publication date: 20200320