CN110836796A

CN110836796A - Sample application method

Info

Publication number: CN110836796A
Application number: CN201810942212.XA
Authority: CN
Inventors: 丁建文
Original assignee: Hunan Aivin Medical Technology Co Ltd
Current assignee: Hunan Aivin Medical Technology Co Ltd
Priority date: 2018-08-17
Filing date: 2018-08-17
Publication date: 2020-02-25

Abstract

The invention discloses a sampling method, which comprises the following steps: s1: adding a sample, namely obtaining the sample to be detected and adding the sample into an externally-arranged extrudable pipe body; s2: sample treatment, namely adding diluent into a tube body, shaking the tube body to uniformly mix the sample and the diluent, and treating the mixed liquid of the sample and the diluent in the tube body to form saturated saline; s3: and standing and sample extruding, wherein the pipe body is positively kept for a set time to enable the eggs to float on the surface of the saturated saline water, and the pipe body is extruded to enable the eggs floating on the surface of the saturated saline water to be transmitted to the outside through an opening arranged above the pipe body. By the sampling method provided by the invention, the worm eggs in the excrement sample suspension can be effectively ensured to be obtained and added into the detection carrier, so that the accuracy of the detection result is effectively ensured.

Description

Sample application method

Technical Field

The invention relates to the field of medical instruments, in particular to a sampling method.

Background

The clinical examination is to detect the body fluid, secretion, excrement, fallen matter and other samples of the patient through visual observation, physical, chemical, instrument or molecular biological method and to emphasize the strict quality control measures taken to ensure the quality of the examination in the whole examination process (before, during and after analysis), so as to provide valuable experimental data for the patient in clinic. In clinical examination, in order to obtain certain physiological data of a patient body, some samples of the human body are often collected to perform various index detection. Before each index data of a sample is detected, the sample generally needs to be diluted, filtered, sampled and the like, and the method specifically comprises the following steps: firstly, collecting a required sample through a collecting device; then the sample is placed in a dilution and filtration device for dilution and filtration, and finally the prepared sample suspension is added into a detection carrier for detection.

In actual operation, although the fecal specimen contains eggs, the eggs are randomly distributed in the fecal sample suspension after the fecal sample is diluted and stirred, so that when the fecal sample is added into the detection carrier, the sample suspension without the eggs may be added into the detection carrier, so that the detection carrier does not contain the eggs, and the accuracy of the detection result is greatly influenced.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a sampling method, and the sampling method can effectively ensure that the ova in the excrement sample suspension are obtained and added into a detection carrier through the structural design of the sampling method, so that the accuracy of a detection result is effectively ensured.

A method of sampling comprising the steps of:

s1: adding a sample, namely obtaining the sample to be detected and adding the sample into an externally-arranged extrudable pipe body;

s2: sample treatment, namely adding diluent into a tube body, shaking the tube body to uniformly mix the sample and the diluent, and treating the mixed liquid of the sample and the diluent in the tube body to form saturated saline;

s3: and standing and sample extruding, wherein the pipe body is positively kept for a set time to enable the eggs to float on the surface of the saturated saline water, and the pipe body is extruded to enable the eggs floating on the surface of the saturated saline water to be transmitted to the outside through an opening arranged above the pipe body.

Preferably, in step S2, the sample is diluted with saturated saline.

Preferably, in step S2, the sample is diluted with distilled water, and then a salt is added to the mixture of the sample and the diluent to form a saturated saline solution.

Preferably, in step S3, a reservoir is provided around the opening above the tube, squeezed into the reservoir, and then spotted into the sample carrier by an external pipettor.

Preferably, in step S3, a sampling tube is connected to the opening above the tube body, and the sample is directly sampled by using the sampling tube.

Preferably, in step S3, a support protrusion is provided on the periphery of the opening above the tube, and the smear is directly spotted by the support protrusion.

Preferably, in step S2, a detachable salt storage frame is disposed inside the tube, and salt particles for producing saturated brine are stored in the salt storage frame in advance.

Preferably, the bottom of the salt storage frame is provided with a sampling spoon, and the sampling spoon is broken off to take salt particles from the salt storage frame during saturated salt water preparation.

Preferably, in step S2, the ratio of the saturated brine to be added is converted from the volume ratio of the tubular body.

The invention has the beneficial effects that: when the sampling method provided by the invention is specifically implemented, only the sample suspension needs to be added into the inner cavity of the tube body, then the sample forms saturated saline, and then the characteristic that the specific gravity of the saturated saline is greater than that of the eggs is utilized, so that the eggs gather on the surface of the saturated saline, at the moment, the extrudable tube body is extruded, the eggs on the surface of the saturated saline flow upwards along with the extrusion of the tube body until the eggs are discharged out of the tube body along with the sample guide tube and then added into the detection carrier, and therefore, the eggs in the excrement sample suspension can be ensured to be obtained to be added into the detection carrier, and the accuracy of the detection result is effectively ensured.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

Fig. 1 is a schematic flow chart of a sampling method according to an embodiment of the present invention.

Detailed Description

In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments.

All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Referring to fig. 1, fig. 1 provides a specific embodiment of a spotting method of the present invention, wherein fig. 1 is a schematic flow chart of the spotting method disclosed in the embodiment of the present invention.

As shown in fig. 1, the present embodiment provides a spot-sampling method, which uses a floating aggregation method to obtain eggs, and can effectively ensure that the eggs in the fecal sample suspension are obtained to be added into the detection carrier, thereby effectively ensuring the accuracy of the detection result.

The sampling method provided by the embodiment comprises the following steps:

In summary, when the spotting method provided by the invention is specifically implemented, only the sample suspension needs to be added into the inner cavity of the tube body, then the sample forms saturated saline, and then the characteristic that the specific gravity of the saturated saline is greater than that of the eggs is utilized, so that the eggs can gather on the surface of the saturated saline, at the moment, the squeezable tube body is squeezed, the eggs on the surface of the saturated saline can flow upwards along with the squeezing of the tube body until the eggs are discharged out of the tube body along with the sample guide tube and then added into the detection carrier, and therefore, the eggs in the fecal sample suspension can be ensured to be obtained to be added into the detection carrier, and the accuracy of the detection result is effectively ensured.

In this embodiment, to further facilitate the processing of the sample, it is preferable that the sample is diluted with saturated saline in step S2.

Of course, the following operation flow may also be adopted: in step S2, the sample is diluted with distilled water, and then a salt is added to the mixture of the sample and the diluent to form a saturated saline solution.

In this embodiment, in order to further facilitate the transfer of the processed sample extruded from the tube body to the detection carrier for detection, it is preferable that in step S3, a storage groove is provided at the periphery of the opening above the tube body, the sample is extruded into the storage groove, and then the sample is spotted onto the sample carrier by an external pipettor.

Of course, the following may also be employed:

In this embodiment, in order to further facilitate the addition of salt particles to the sample and the diluent to form saturated brine, preferably, in step S2, a detachable salt storage frame is disposed inside the tube, and the salt particles for manufacturing saturated brine are stored in the salt storage frame in advance. Preferably, the bottom of the salt storage frame is provided with a sampling spoon, and the sampling spoon is broken off to take salt particles from the salt storage frame during saturated salt water preparation.

The above description details a sampling method provided by the present invention. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.

Claims

1. A method of sampling, comprising the steps of:

2. The spotting method of claim 1, wherein in step S2, the sample is diluted with saturated saline.

3. The spotting method of claim 1, wherein in step S2, the sample is diluted with distilled water, and then a salt is added to the mixture of the sample and the diluent to form a saturated saline solution.

4. The spotting method according to claim 1, wherein in step S3, a reservoir is provided on the periphery of the upper opening of the tube, a sample is squeezed into the reservoir, and then spotted into the sample carrier by an external pipette.

5. The spotting method of claim 1, wherein in step S3, a spotting tube is attached at an upper opening of the tube body, and direct spotting is performed by using the spotting tube.

6. The spotting method of claim 1, wherein in step S3, a support protrusion is provided on the periphery of the upper opening of the tube, and the smear is directly spotted through the support protrusion.

7. The spotting method of claim 1, wherein in step S2, a detachable salt storage frame is provided inside the tube, and salt particles for producing saturated brine are stored in the salt storage frame in advance.

8. The spotting method of claim 7 wherein the bottom of the salt storage frame is provided with a sampling spoon, and the sampling spoon is broken to scoop salt particles from the salt storage frame when saturated brine is produced.

9. The spotting method according to claim 1, wherein in step S2, the proportion of saturated brine to be added is converted from the volume proportion of the tube body.