CN110818480A

CN110818480A - Plant organic converzyme degradation agent and preparation and use methods thereof

Info

Publication number: CN110818480A
Application number: CN201911097517.6A
Authority: CN
Inventors: 唐豪臻
Original assignee: Chengdu Dingtai Xinyuan Agricultural Technology Co Ltd
Current assignee: Chengdu Bisen Ecological Agriculture Co.,Ltd.
Priority date: 2019-11-11
Filing date: 2019-11-11
Publication date: 2020-02-21

Abstract

The invention discloses a plant organic converting enzyme degradation agent, which comprises boric acid, zinc chloride, ammonium sulfate, monopotassium phosphate, potassium nitrate, copper citrate, glucose-6-phosphate dehydrogenase, amino acid, magnesium chloride, manganese nitrate, ferrous sulfate, urea, zinc sulfate and water; the degradation agent can effectively remove pesticide residues in plants, and can simultaneously solidify heavy metals in soil for plant growth, so that the amount of the pesticide residues and the heavy metals in the plants can be effectively reduced, and the plants can reach the detection standard of low pesticide residues and low heavy metals.

Description

Plant organic converzyme degradation agent and preparation and use methods thereof

Technical Field

The invention relates to the technical field of degradation agents, in particular to a plant organic convertase degradation agent, and further relates to a preparation method and a use method of the plant organic convertase degradation agent.

Background

Improper utilization or improper management of farmland soil by high-strength planting is a main reason for continuous attenuation of organic matters in soil of main grain production areas in China and decline of farmland soil fertility; by applying ammonium nitrate, ammonium phosphate and compound fertilizer for a long time, the content of arsenic in soil can reach 50-60mg/kg, the content of effective heavy metal in soil can be increased along with the increase of heavy metal entering the soil, and the phenomenon that the heavy metal of crops exceeds the standard can be continuously caused along with the increase of the absorbed amount of crops; heavy metals such as cadmium, arsenic and the like in soil cannot be naturally decomposed, the higher the content of the heavy metals is, the more heavy metals enter crops, and the content of the heavy metals in the crops can be reduced only by controlling the absorption of the heavy metals by the crops.

In the planting industry, in order to ensure and increase yield, the crops are sprayed with pesticides in a normal state, but the pesticides contain various heavy metals, so that although the insect pests can be reduced by spraying the pesticides, the heavy metals remain in farmlands, the crops grow to absorb the heavy metals, and excessive pesticide residues cause the pesticide residues and the heavy metals in plants to fail to meet the detection standard of European Union and can not meet the requirements of organic agricultural products.

Plant Organic convertase pesticide residue degradation technology (Organic conversion bio-enzyme pesticide residue degradation technology in plants) is hereinafter referred to as degradation technology, and a plurality of sets of plant Organic convertase degradation agents (hereinafter referred to as degradation agents) and biological signal agents (hereinafter referred to as signal agents) enable agricultural products to reach the pesticide residue-free standard (0.00mg/Kg) and reach the European Union detection standard; the degradation technology has the following advantages: the method can be summarized into 'three or more', and specifically, the method does not need to increase any agricultural facilities, does not need three years of soil conversion, does not need to increase the additional investment of farmers, can be used on the land polluted by pesticide and fertilizer, can be used on the land needing to continue using pesticide and fertilizer, can be used in the growth process of plants, and can produce high-quality products in one or more production periods.

The application number is: CN201711290110.6, publication no: the invention of CN107840721A discloses a biological organic fertilizer for passivating soil heavy metals, which is prepared by cutting and crushing edible fungus residues, uniformly mixing the edible fungus residues with animal wastes, drying the mixture in the sun, adding a compound degradation agent for fermentation and degradation, then mixing and adding a soil modification material, a heavy metal passivation material, urea, monoammonium phosphate, a trace element fertilizer and a curing agent, carrying out curing treatment, and finally carrying out extrusion granulation and grinding the mixture into powder. According to the invention, the heavy metal passivation material is adopted to passivate the heavy metal in the organic fertilizer and the heavy metal originally contained in the soil, so that the applied organic fertilizer can not cause the heavy metal pollution of the soil, and can repair the heavy metal pollution of the soil; however, the invention cannot reduce pesticide residue in plants, and the plants still have pesticide residue.

The application number is: CN201610816188.6, publication no: the invention patent of CN106244166A discloses a degrading agent for agricultural harmful heavy metals and pesticide and fertilizer residues; the pesticide residue can be effectively degraded, and the pesticide residue has the effects of oxidation resistance and integrated detoxification; harmful active oxygen and free radicals are removed, and the metabolic activity of plants is improved; the heavy metal can be quickly and effectively removed; soil hardening can be effectively relieved; can provide nutrition for the growth of crops and improve the metabolism level; however, the effect of removing pesticide residues and heavy metals is not obvious, and needs to be further improved.

Disclosure of Invention

The invention aims to provide a plant organic convertase degrading agent, which can effectively remove pesticide residues in plants and effectively reduce the amount of pesticide residues and heavy metals in the plants, so that the content of the pesticide residues and the heavy metals in the plants reaches the detection standard; meanwhile, the preparation method realizes the preparation of the plant organic convertase degradation agent, and the preparation process is simple.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

a plant organic convertase degrading agent is characterized by comprising the following raw materials in percentage by mass: 0.51 to 0.61 percent of boric acid, 0.50 to 0.56 percent of zinc chloride, 1.43 to 1.58 percent of ammonium sulfate, 0.48 to 0.53 percent of monopotassium phosphate, 3.05 to 3.37 percent of potassium nitrate, 3.11 to 3.43 percent of cupric citrate, 0.27 to 0.29 percent of glucose-6-phosphate dehydrogenase, 13.55 to 14.98 percent of amino acid, 1.18 to 1.30 percent of magnesium chloride, 2.30 to 2.50 percent of manganese nitrate, 0.14 to 0.16 percent of ferrous sulfate, 0.48 to 0.53 percent of urea, 1.49 to 1.64 percent of zinc sulfate and the balance of water.

Wherein the raw materials comprise the following components in percentage by mass: 0.58% of boric acid, 0.53% of zinc chloride, 1.5% of ammonium sulfate, 0.5% of monopotassium phosphate, 3.21% of potassium nitrate, 3.27% of copper citrate, 0.28% of glucose-6-phosphate dehydrogenase, 14.26% of amino acid, 1.24% of magnesium chloride, 2.42% of manganese nitrate, 0.15% of ferrous sulfate, 0.5% of urea and 1.56% of zinc sulfate.

The compound amino acid composition comprises 17.776% of aspartic acid, 5.058% of threonine, 5.772% of serine, 23.754% of glutamic acid, 13.520% of glycine, 4.455% of alanine, 0.071% of cysteine, 3.842% of valine, 0.042% of methionine, 2.030% of isoleucine, 2.622% of leucine, 0.876% of tyrosine, 1.366% of phenylalanine, 1.932% of lysine, 6.610% of arginine and the balance of proline.

The preparation method of the plant organic converting enzyme degradation agent comprises the following steps:

step 1: weighing, namely weighing the following raw materials in percentage by mass at normal temperature: 1.24% of magnesium chloride, 1.5% of ammonium sulfate, 0.5% of monopotassium phosphate, 2.42% of manganese nitrate, 0.15% of ferrous sulfate, 0.5% of urea, 1.56% of zinc sulfate, 0.53% of zinc chloride, 3.21% of potassium nitrate and 50% of deionized water; mixing the weighed raw materials of the components, and fully stirring to prepare a liquid stock solution for later use;

step 2: weighing 20% of deionized water at normal temperature as a solvent for later use;

and step 3: weighing 0.28 percent of glucose-6-phosphate dehydrogenase and 14.26 percent of amino acid mixed solution, and mixing to prepare a biological agent for later use;

and 4, step 4: injecting the prepared liquid stock solution and the solvent into a configuration tank, continuously stirring for 2-4 hours under the conditions that the pressure P is 1000-plus-energy 5000Pa and the temperature T is 65-80 ℃, and carrying out condensation reflux at 0 ℃;

and 5: weighing 0.58% of boric acid and 3.27% of copper citrate as regulators, gradually injecting the regulators into a preparation tank, and regulating the pH value of a solution to be 6-7;

step 6: and (3) the solution prepared in the step (5) is fused in a dark place, the biological preparation is fully stirred after being injected, sampling detection is carried out, a finished product is prepared by filtering through a filtering device after the biological preparation is detected to be qualified, and the filtered precipitate is recovered.

And 7: packaging the finished product and packaging the finished product.

In step 4, the pressure P is 3000Pa, the temperature T is 70 ℃, and the stirring time is 3 hours.

In step 5, the PH of the solution was adjusted to 6.5.

Wherein, in the step 6, the light-shielding time is 24H, and the light-shielding temperature is 20-30 ℃.

The application method of the plant organic converting enzyme degradation agent is provided below, specifically a spraying method, which specifically comprises the following steps:

step 1: diluting, namely diluting the plant organic converting enzyme degradation agent and water according to the ratio of 1: 100-500;

step 2: and (3) spraying, namely spraying the diluted plant organic convertase degrading agent in the step (1) on the leaf surfaces of the plants.

When spraying, the diluted plant organic convertase degrading agent is sprayed on the leaf surfaces of the plants.

Wherein, when spraying on fruit tree plants, the organic convertase degradation agent can be diluted according to the ratio of 1: 500, and is sprayed once in the leaf-expanding period of the fruit trees and is sprayed once in the small fruit period; and then spraying twice in the fruit expansion period, wherein the interval time between the two times is 15 days, the spraying is carried out simultaneously with the pesticide, and the spraying time is selected at the evening of sunny days.

The following provides another use method of the plant organic converting enzyme degradation agent, in particular to a seed dressing method, which is characterized by comprising the following steps:

step 1: diluting, namely diluting the plant organic converting enzyme degradation agent and water according to the ratio of 1: 100-300,

step 2: mixing seeds, namely uniformly spraying the diluted plant organic converting enzyme degradation agent on the surfaces of the seeds, fully stirring the seeds to ensure that the surfaces of the seeds are uniformly covered with the plant organic converting enzyme degradation agent, and tightly covering the stirred seeds for 3-4 h;

and 4, step 4: and (6) sowing.

The seeds are transplanted after sprouting and leaf growing, and after the seeds are planted, the diluted degradation agent is sprayed every 7 to 10 days in the growth period of the seeds, and the degradation agent is continuously sprayed for 6 to 8 times.

Another method of using the plant organic converting enzyme degradation agent, which is specifically a root irrigation method, is provided below, comprising the steps of:

step 1: diluting, namely diluting the plant organic converting enzyme degradation agent and water according to the ratio of 1: 200-300;

step 2: spraying diluted plant organic convertase degradation agent to wet seed blocks or seed soaking seedlings for 8-12Min, transplanting the plants to root, irrigating roots with the diluted plant organic convertase degradation agent, spraying the diluted plant organic convertase degradation agent once every 7-10 days in the plant growth period, and continuously spraying for 4-6 times.

Compared with the prior art, the invention has the following beneficial effects:

1. in the invention, the synergistic effect of boric acid, zinc chloride, ammonium sulfate, monopotassium phosphate, potassium nitrate, copper citrate, glucose-6-phosphate dehydrogenase and amino acid (aspartic acid, serine, glycine, methionine, isoleucine and proline) can effectively remove pesticide residues; the magnesium chloride, the zinc chloride, the ammonium sulfate and the amino acid (arginine, phenylalanine and cysteine) have synergistic effect, so that the organic matters in the soil are increased, the soil structure is improved, the formation of a soil mesh structure is promoted, the soil exchange capacity is improved, the permeability is increased, and the water and fertilizer retention capacity is improved; activating geography, improving soil fertility, purifying environmental viruses and creating a good growing environment for plants; can effectively reduce the pesticide residue and heavy metal content in the crop body.

2. The fertilizer disclosed by the invention comprises monopotassium phosphate, potassium nitrate, magnesium chloride, boric acid, manganese nitrate, ferrous sulfate, zinc chloride, urea, copper citrate and amino acids (threonine, glutamic acid, alanine, cysteine, valine, methionine, isoleucine, leucine, tyrosine, lysine, arginine and proline), and can increase the yield of crops and improve the quality of the crops, wherein the yield of the vegetables is increased by 30-50%, the yield of the grains is increased by 20-30%, and the yield of fresh fruits is increased by 25-40%; the amount of trace elements supplied to the storage organs from roots and leaves can be regulated, the ratio and balance of macroelements and trace elements can be regulated, the activity of enzyme can be enhanced and promoted, the cell division and metabolism can be promoted, the polysaccharide can be converted into soluble monosaccharide, starch, protein, fat and various vitamins, the flower bud differentiation, flowering and fruiting of crops such as melon, fruit and vegetable can be promoted, and the sugar content can be increased.

3. The boric acid, the zinc sulfate, the copper citrate and the amino acid (serine/isoleucine) can achieve the sterilization effect under the synergistic effect, and the mechanism is to destroy pathogenic bacteria, solidify protein, precipitate and deteriorate, and inactivate enzymes of cell walls of the pathogenic bacteria to achieve the sterilization purpose; has obvious effect on fungi, bacteria and viruses, such as ginger rot, root rot, pepper virus disease, leaf spot and the like.

4. The amino acid (proline and serine), the monopotassium phosphate and the potassium nitrate can effectively improve the frost resistance, drought resistance and waterlogging resistance of crops; in the drought period, the invention can be used for inducing and producing a large amount of cytokinin and vitamin and maintaining the higher level, thereby ensuring higher photosynthesis rate, easily promoting the development of plant root systems and stimulating the division and growth of extreme meristematic cells of the root systems; in the low-temperature period, the invention can effectively activate the chitinase and the protease in the plant body, comprehensively improve the content of amino acid and chitin, increase the content of unsaturated fatty acid in cell membranes and ensure that the cell membranes can normally grow at the low temperature; and can prevent root system from rotting and white root from aging, and has better waterlogging resistance.

5. The amino acid (glycine, threonine and phenylalanine) can increase the surface density of crops, generate sealing to prevent bacteria from immersing, reduce bacteria or kill bacteria, increase the total amount of soluble solid matters, carbohydrate and sucrose, reduce the physiological weight loss in the process of storage and transportation and enhance the corrosion resistance; can effectively improve the fresh-keeping capacity of crops.

Drawings

FIG. 1 is a schematic view of the principle and flow of the production equipment required for the preparation of the present invention.

FIG. 2 is a partial test report of the spray-planted kiwi fruit of the present invention.

FIG. 3 is a partial detection report of the pesticide residue content of the fruit after the kiwi fruit is sprayed and planted according to the present invention.

FIG. 4 is a report of the test results of the rice seeds dressing and planting method of the present invention.

FIG. 5 is a partial detection report of Chinese yam after root irrigation and planting.

Reference numerals: 1 preparation container, 2 metering tanks, 3 configuration tanks, 4 condensing appliances, 5 filtering devices, 6 finished product storage tanks, 7 packaging machines and 8 sampling ports.

Detailed Description

The present invention will be further described with reference to the following examples, which are intended to illustrate only some, but not all, of the embodiments of the present invention. Based on the embodiments of the present invention, other embodiments used by those skilled in the art without any creative effort belong to the protection scope of the present invention.

Example 1

A plant organic convertase degrading agent comprises the following raw materials in percentage by mass:

0.51 to 0.61 percent of boric acid,

0.50 to 0.56 percent of zinc chloride,

1.43 to 1.58 percent of ammonium sulfate,

0.48 to 0.53 percent of monopotassium phosphate,

3.05 to 3.37 percent of potassium nitrate,

3.11 to 3.43 percent of copper citrate,

0.27 to 0.29 percent of glucose-6-phosphate dehydrogenase,

13.55 to 14.98 percent of amino acid,

1.18 to 1.30 percent of magnesium chloride,

2.30 to 2.50 percent of manganese nitrate,

0.14 to 0.16 percent of ferrous sulfate,

0.48 to 0.53 percent of urea,

1.49 to 1.64 percent of zinc sulfate,

the balance being water.

Further optimizing, wherein the specific mass percentages of the raw materials are as follows:

0.58% of boric acid, 0.53% of zinc chloride, 1.5% of ammonium sulfate, 0.5% of monopotassium phosphate, 3.21% of potassium nitrate, 3.27% of copper citrate, 0.28% of glucose-6-phosphate dehydrogenase and 14.26% of amino acid; 1.24% of magnesium chloride, 2.42% of manganese nitrate, 0.15% of ferrous sulfate, 0.5% of urea, 1.56% of zinc sulfate and the balance of water.

The amino acid comprises 17.776% of aspartic acid, 5.058% of threonine, 5.772% of serine, 23.754% of glutamic acid, 13.520% of glycine, 4.455% of alanine, 0.071% of cysteine, 3.842% of valine, 0.042% of methionine, 2.030% of isoleucine, 2.622% of leucine, 0.876% of tyrosine, 1.366% of phenylalanine, 1.932% of lysine, 6.610% of arginine and the balance of proline.

The degradation agent can effectively remove pesticide residues in the plant body, and can simultaneously solidify heavy metals in the plant growth soil, effectively reduce the pesticide residues and the heavy metals in the plant body, and enable the plant body to reach the detection standard of low pesticide residues and low heavy metals. The preparation process of the plant organic convertase degrading agent comprises the following steps:

the production equipment required for converting the enzyme degradation agent by the preparation machine is as follows, and the attached figure 1 of the specification refers to:

the device mainly comprises a weighing device, a preparation container 1, a metering tank 2, a configuration tank 3, a condensing appliance 4, a filtering device 5, a finished product storage tank 6 and a packaging machine 7;

wherein, the weighing device is used for weighing each component raw material; the preparation container 1 is dissolved in the dissolving raw material; the metering tank 2 is used for metering the solution; the preparation tank 3 is a reaction container and is used for preparing solution, and a stirring device is arranged in the preparation tank; the condensing device 4 is used for condensing, and the filtering device 5 is used for filtering the solution; the finished product storage tank 6 is used for temporarily storing the degradation agent, and the packaging machine 7 is used for packaging the finished product; each component can be realized by the equipment in the prior art.

In the actual preparation, three preparation containers 1 and three metering tanks 2 are respectively arranged, wherein the three preparation containers 1 are respectively used for preparing liquid stock solution, solvent and biological preparation, the metering tank 2 is used for metering the amounts of the liquid stock solution, the solvent and the biological preparation, and then the liquid stock solution, the solvent and the biological preparation are subjected to reaction configuration in a configuration tank 3; and sampling detection is carried out through a sampling port 8 between the configuration groove 3 and the filtering device 5; the prepared degradation agent is filtered by the filtering device 5, and is temporarily stored by the finished product storage tank 6 and then packed and packaged by the packing machine 7.

The production equipment in the invention is further explained as follows:

as shown in fig. 1, three preparation containers 1 are respectively connected to three metering tanks 2, the lower ends of the three metering tanks 2 are connected to a configuration tank 3, the upper ends of the three metering tanks 2 are connected to a finished product storage tank 6, the configuration tank 3 is simultaneously connected to a condenser 4 and a filter device 5, the condenser 4 is connected to the finished product storage tank 6, the filter device 5 is connected to the finished product storage tank 6, the finished product storage tank 6 is connected to a packing machine 7, and a sampling port 8 is provided between the configuration tank 3 and the filter device 5.

Wherein, the finished product consists of two parts, one part is obtained by condensing by a condensing device 4, and the other part is obtained by filtering by a filtering device 5.

The specific preparation process is as follows;

and 4, step 4: injecting the prepared liquid stock solution and a solvent into a configuration tank, continuously stirring for 3 hours under the conditions that the pressure P is 3000Pa and the temperature T is 70 ℃, and carrying out condensation reflux at 0 ℃; it should be noted that, when condensing, it is necessary to ensure that the condensation is carried out at 0 ℃;

and 5: weighing 0.58% of boric acid and 3.27% of copper citrate as regulators, gradually injecting the regulators into a preparation tank, and regulating the pH value of a solution to be 6.5;

And 7: packaging the finished product and packaging the finished product.

In step 6, the dark time is 24H, the dark temperature is 20-30 ℃, and the actual dark temperature is 25 ℃.

In step 3, the amino acid mixed solution is prepared by mixing aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cysteine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, arginine and proline.

Thus, the preparation of the plant organic convertase degrading agent can be finished, and the process is simple, convenient and fast and is suitable for mass production.

Example 2

The embodiment is further optimized on the basis of embodiment 1, and in the embodiment, the specific mass percentages of the raw materials of the plant organic converting enzyme degradation agent are as follows:

0.61% of boric acid, 0.56% of zinc chloride, 1.58% of ammonium sulfate, 0.53% of monopotassium phosphate, 3.37% of potassium nitrate, 3.43% of copper citrate, 0.29% of glucose-6-phosphate dehydrogenase and 14.98% of amino acid; 1.30 percent of magnesium chloride, 2.50 percent of manganese nitrate, 0.16 percent of ferrous sulfate, 0.53 percent of urea and 1.64 percent of zinc sulfate; the balance being water.

In this example, in step 4, the pressure P is 1000Pa, the temperature T is 65 ℃, and the stirring time is 2 hours; the solution was adjusted to PH 6.

Example 3

0.51% of boric acid, 0.50% of zinc chloride, 1.43% of ammonium sulfate, 0.48% of monopotassium phosphate, 3.05% of potassium nitrate, 3.11% of copper citrate, 0.27% of glucose-6-phosphate dehydrogenase and 13.55% of amino acid; 1.18 percent of magnesium chloride, 2.30 percent of manganese nitrate, 0.14 percent of ferrous sulfate, 0.48 percent of urea and 1.49 percent of zinc sulfate; the balance being water.

In this example, in step 4, the pressure P is 5000Pa, the temperature T is 80 ℃, and the stirring time is 4 hours.

Example 4

The embodiment is a use method of a plant organic converting enzyme degradation agent, which specifically comprises the following steps:

a spraying method of a plant organic convertase degrading agent.

The method comprises the following specific steps:

step 1: diluting, the plant organic converting enzyme degradation agent prepared in the

embodiment

1, 2 or 3 and water are diluted according to the proportion of 1: 100, 1: 250 or 1: 500.

It should be noted that the fruit spraying and vegetable spraying modes are slightly different:

wherein, the spraying mode of the fruit tree plants is as follows:

spray application example 1

(1) The following example is the planting of macaca:

diluting the plant organic convertase degrading agent in a ratio of 1: 500; the rapid evaporation of the degradation agent with overhigh temperature is avoided by selecting evening during spraying.

The spraying method comprises the following specific steps:

s1: in the leaf-expanding period of the kiwi fruits, the diluted solution according to the ratio of 1: 500 is sprayed on the leaf surfaces once.

S2: spraying the fertilizer to the leaf surface once in the small fruit period.

S3: in the fruit expansion period, the spraying is carried out twice, and the interval time between the two times of spraying is 15 days.

When the degradation agent is sprayed, the plant organic converting enzyme degradation agent and the pesticide can be mixed and sprayed; and spraying pesticides normally in the planting process.

After the kiwi fruits are sprayed on the leaf surfaces by the method, the kiwi fruits are detected, and the detection result is as follows:

sample numbering

Sample name

Detecting items

Detection method

Result data

Unit of

Detection lower bound

2014009427

Kiwi fruit

Death by poisonTick (Bolus)

GB/T19648-2005

Not detected out

mg/Kg

0.01

2014009427

Kiwi fruit

Abamectin and its preparation method

SN/T1973-2007

Not detected out

mg/Kg

0.01

2014009427

Kiwi fruit

Thiophanate-methyl

Sop

Not detected out

mg/Kg

0.01

2014009427

Kiwi fruit

Lead (II)

DB53/T288-2009

Not detected out

mg/Kg

0.03

2014009427

Kiwi fruit

Arsenic (As)

DB53/T288-2009

Not detected out

mg/Kg

0.03

2014009427

Kiwi fruit

Mercury

EPA7473*

Not detected out

mg/Kg

0.001

Above-mentioned data can be clearly known, through choosing the kiwi fruit and carry out the spraying after, the pesticide residue in the fruit all is less than and detects the low limit, and the kiwi fruit is chosen hardly to contain common heavy metal in the fruit, and its concrete testing result refers to description attached figure 2 and 3.

Spray application example 2

(2) The following takes mango planting as an example:

s1: diluting the prepared organic convertase degrading agent according to a ratio of 1: 500, spraying the mango leaves once after picking, recovering the nutrition of the tree, preventing frost damage and increasing the annual-rotation flower quantity;

s2: after turning green of the fruit tree in the year, after the new leaves are unfolded, spraying the diluted organic convertase degrading agent once; dry and hot wind resistance, cold and wet wind resistance, overcast and rainy resistance, and hollow flower and hollow fruit resistance;

s3: spraying for 2 times after the small fruit period, wherein the interval time is 15 days; the activity of leaves is maintained, the photosynthetic capacity is increased, and the enzyme activity is increased;

s4: spraying for 2 times after the fruit swelling period, wherein the interval time is 15 days each time; increase enzyme amount and activity, resist diseases, reduce pesticide residue, increase weight of single fruit, and improve quality.

S5: spraying for 1 time after picking the bag; the surface smoothness, brightness and color of the fruit are increased, and the preservation period is prolonged.

Spray application example 3

(3) The cherry planting is taken as an example as follows:

s1: diluting plant organic convertase degrading agent with water at a ratio of 1: 500, spraying leaf surface after leaf development of cherry tree, and spraying and sterilizing trunk when leaf surface is sprayed;

s2: spraying the foliage once after the small fruit period; continuously spraying the fertilizer twice in the fruit expansion period, wherein the interval of each time is 12 days;

s3: spraying once in the fruit coloring period.

Spray application example 4

The vegetable spraying mode is as follows:

diluting the prepared plant organic convertase degrading agent with water according to a ratio of 1: 100, spraying the plant organic convertase degrading agent once every 7 days after the vegetables are planted, and continuously spraying the plant organic convertase degrading agent for 4-6 times.

It should be noted that the spraying method is not only suitable for the above crops, but also suitable for the following crops, and the spraying method can be used for foliage spraying as long as the plants have leaves capable of photosynthesis.

Such as: cereal crops: wheat, sorghum, corn, and the like;

leguminous crops: soybeans, beans, cowpeas, lentils, broad beans, lima beans, peas, and the like;

dioscorea species: potato, yam, ginger, taro, yam bean, sweet potato, konjak, stachys sieboldii, kudzu, jerusalem artichoke, valency, canna, etc.;

oil crops: rape, peanut, sesame, etc.;

sugar crops: sugar cane, sugar beet, and the like;

preference for crops: tea, tobacco, coffee, cocoa, etc.;

leaf vegetables: spinach, fennel, leaf beet, celery, crowndaisy chrysanthemum, lettuce, endive, chicory, winter cabbage, shepherd's purse, burclover and spinach elm; amaranth, tetragonia, basella rubra, perilla, gynura bicolor and basil; asparagus lettuce, mint, endive, parsley, dill, chrysanthemum camphor and the like.

Melons: cucumber, wax gourd, pumpkin, winter squash, pumpkin, gourd, melon, towel gourd, balsam pear, bottle gourd, Japanese melon, snake gourd and chayote;

scallion and garlic vegetables: leek, scallion, onion, garlic, leek, chive, shallot, chives, and scallion;

tea trees: small leaf tea, big leaf tea, vine tea, woody tea, etc.;

chinese medicinal materials: pseudo-ginseng, Chinese yam, ginseng, gastrodia elata, poria cocos, fritillaria, semen cassiae, ginkgo, dark plum, motherwort, herba lycopi, cistanche, rose, saussurea involucrate, honeysuckle, cortex dictamni, cortex albiziae, mangnolia officinalis, caulis polygoni multiflori, acanthopanax, sargentgloryvine stem, poria cocos, cordyceps sinensis, kelp, perilla leaf, folium artemisiae argyi, folium isatidis and the like;

fruits: apple, pear, Chinese flowering apple, hawthorn, papaya, peach, plum, apricot, plum, chestnut, walnut, hickory nut, hazelnut, pistachio nut, ginkgo, torreya, grape, currant, raspberry, strawberry, papaya, pomegranate, ginseng fruit and the like, mandarin orange, tangerine, orange, pomelo, lemon, banana, pyrus ussuriensis, longan, lychee, olive, waxberry, coconut, guava, carambola, jujube, persimmon, fig and the like.

The invention can be applied to the plants, and can effectively reduce the pesticide residue and the heavy metal content in the plants after being applied.

Example 5

The embodiment is a use method of a plant organic converting enzyme degradation agent, which specifically comprises the following steps: a seed dressing method of a plant organic convertase degrading agent.

The method specifically comprises the following steps:

step 1: diluting, the plant organic converting enzyme degradation agent prepared in the example 1 and water are diluted according to the ratio of 1: 100-300, wherein the ratio can be: 1: 100, 1: 150 or 1: 300.

and 4, step 4: and (6) sowing.

Seed dressing example 1

(1) The following is an example of rice planting:

in the embodiment, the plant organic convertase degradation agent and water are diluted according to the ratio of 1: 100; uniformly spraying the diluted plant organic convertase degradation agent on seeds, stirring the seeds to enable the degradation agent to be uniformly attached to the surfaces of the seeds, then carrying out seed closing on the stirred seeds, wherein the seed closing time is 3.5 hours, enabling the seeds to quickly recover and germinate, and sowing the seeds after seed closing;

the seed smoldering time of the seeds can be confirmed according to the volume of the single seeds, the small volume can reduce the seed smoldering time, and the large volume can properly increase the seed smoldering time; for example, cabbage seeds can be planted in a stuffy manner for 3 hours, broad beans and big beans can be planted in a stuffy manner for 5 hours, and pea seeds can be planted in a stuffy manner for 4 hours.

The seeds after sowing, sprouting and long leaf growing are transferred, and after the seeds are fixedly planted, diluted degradation agents are sprayed, the degradation agents are sprayed once every 7-10 days, and the seeds are continuously sprayed for 6-8 times.

The detection results of the rice cultured after seed dressing are as follows, and the specific safety detection results refer to the attached figure 4 of the specification:

wherein, the mercury content is lower than 0.005 mg/Kg;

the lead content is lower than 0.05 mg/Kg;

the chromium content is lower than 0.1 mg/Kg;

the cadmium content is lower than 0.01 mg/Kg;

and the number of the first and second electrodes,

pesticide content values of all scans are less than a content Limit (LOQ) by pesticide residue scan (GC); pesticide residue scanning (LC) except that the content of tricyclazole exceeds standard, the pesticide values of other scans are all less than the content Limit (LOQ); the rice contains less heavy metals and less pesticide residues.

Seed dressing example 2

(2) Spinach planting is taken as an example below:

diluting the prepared plant organic convertase degrading agent according to the ratio of 1: 200, uniformly spraying the diluted plant organic convertase degrading agent on spinach seeds, stirring the sprayed seeds to uniformly adhere the degrading agent to the surface of each seed, then placing the stirred seeds in seed smoldering equipment, performing seed smoldering for 3.6 hours at the constant temperature of 38 ℃, rapidly reviving the seeds to sprout in a proper humidity range, and sowing the seeds after seed smoldering; and transplanting the sown and germinated long-leaf seeds, planting the seeds, spraying the diluted organic convertase degradation agent once every 8 days, and continuously spraying for 7 times.

It should be noted that the seed dressing process is applied to all seed plants, such as: rape, peanut, wheat, sorghum, corn, soybean, kidney bean, cowpea, hyacinth bean, broad bean, lima bean, pea, cucumber, white gourd, pumpkin, squash, spinach and the like.

Example 6

The embodiment is a use method of a plant organic converting enzyme degradation agent, which specifically comprises the following steps: a root irrigation method of a plant organic convertase degradation agent.

The method specifically comprises the following steps:

step 1: diluting, the plant organic converting enzyme degradation agent prepared in the embodiment 1 and water are diluted according to the ratio of 1: 200-300, and the specific ratio can be: 1: 200, 1: 150 or 1: 300.

Step 2: spraying diluted plant organic convertase degrading agent to wet seed blocks or seed soaking seedlings for 8-12Min, transplanting the plants to root, irrigating roots with the diluted plant organic convertase degrading agent, spraying the diluted plant organic convertase degrading agent once every 7-10 days in the growth period of the plants, and continuously spraying for 4-6 times.

Root irrigation example 1

(1) The following takes planting of yam as an example:

diluting the plant organic convertase degradation agent in a ratio of 1: 300 to prepare yam tuberous roots to be planted, soaking the yam tuberous roots to be planted for 11Min, and then transferring the yam tuberous roots;

after the Chinese yam roots, irrigating the roots once by using the diluted plant organic convertase degrading agent;

spraying diluted plant organic convertase degradation agent in the growth period of rhizoma Dioscoreae, spraying once every 8 days, and continuously spraying 5 times in the growth period.

During the growth process of the Chinese yam, the pesticide is sprayed according to the common planting process of the Chinese yam, or the pesticide and the degradation agent can be mixed and then sprayed.

The Chinese yam is detected, the detection result is as follows, and the specific detection result refers to the attached figure 5 of the specification:

the mercury content is lower than 0.005 mg/Kg;

the lead content is lower than 0.05 mg/Kg;

the arsenic content is lower than 0.05 mg/Kg;

the chromium content is lower than 0.1 mg/Kg;

the cadmium content is lower than 0.01 mg/Kg;

and, all scanned pesticide content values are below the limit of content (LOQ);

according to the detection data, the heavy metal and pesticide residue content of the Chinese yam is lower than the quantitative limit.

After the Chinese yam is subjected to root irrigation and the degradation agent is sprayed, the pesticide residue content and the heavy metal content of the Chinese yam are lower than the content Limit (LOQ), the pesticide residue content and the heavy metal content of the Chinese yam are low, and the standard of organic agricultural products is met.

The invention can also be used for planting potatoes, yams, gingers, taros, yam beans, sweet potatoes, konjak, stachys sieboldii, kudzuvine, jerusalem artichoke, valency for vegetables, canna edulis and the like.

It needs to be further explained that, in the practical use, the plant organic convertase degradation agent can be independently sprayed, mixed with seeds or irrigated to roots, and can also be used simultaneously, and the effect of removing pesticide residues and heavy metals is better when the plant organic convertase degradation agent is used simultaneously.

Claims

1. A plant organic convertase degrading agent is characterized by comprising the following raw materials in percentage by mass: 0.51 to 0.61 percent of boric acid, 0.50 to 0.56 percent of zinc chloride, 1.43 to 1.58 percent of ammonium sulfate, 0.48 to 0.53 percent of monopotassium phosphate, 3.05 to 3.37 percent of potassium nitrate, 3.11 to 3.43 percent of cupric citrate, 0.27 to 0.29 percent of glucose-6-phosphate dehydrogenase, 13.55 to 14.98 percent of amino acid, 1.18 to 1.30 percent of magnesium chloride, 2.30 to 2.50 percent of manganese nitrate, 0.14 to 0.16 percent of ferrous sulfate, 0.48 to 0.53 percent of urea, 1.49 to 1.64 percent of zinc sulfate and the balance of water.

2. The plant organic convertase degrading agent according to claim 1, characterized in that the specific mass percentages of the raw materials are as follows: 0.58% of boric acid, 0.53% of zinc chloride, 1.5% of ammonium sulfate, 0.5% of monopotassium phosphate, 3.21% of potassium nitrate, 3.27% of copper citrate, 0.28% of glucose-6-phosphate dehydrogenase, 14.26% of amino acid, 1.24% of magnesium chloride, 2.42% of manganese nitrate, 0.15% of ferrous sulfate, 0.5% of urea and 1.56% of zinc sulfate.

3. The plant organic convertase degrading agent according to claim 1, characterized in that: the amino acid comprises 17.776% of aspartic acid, 5.058% of threonine, 5.772% of serine, 23.754% of glutamic acid, 13.520% of glycine, 4.455% of alanine, 0.071% of cysteine, 3.842% of valine, 0.042% of methionine, 2.030% of isoleucine, 2.622% of leucine, 0.876% of tyrosine, 1.366% of phenylalanine, 1.932% of lysine, 6.610% of arginine and the balance of proline.

4. A preparation method of a plant organic convertase degrading agent is characterized by comprising the following steps:

step 6: the solution prepared in the step 5 is fused in a dark place, the biological preparation is fully stirred after being injected, sampling detection is carried out, a finished product is prepared by filtering through a filtering device after the biological preparation is detected to be qualified, and the filtered precipitate is recycled;

and 7: packaging the finished product and packaging the finished product.

5. The process for producing a plant organic convertase degrading agent according to claim 4, wherein: in step 4, the pressure P is 3000Pa, the temperature T is 70 ℃, and the stirring time is 3 hours.

6. The process for producing a plant organic convertase degrading agent according to claim 4, wherein: in step 5, the solution was adjusted to PH 6.5.

7. The process for producing a plant organic convertase degrading agent according to claim 4, wherein: in step 6, the dark time is 24H, and the dark temperature is 20-30 ℃.

8. A spraying method of a plant organic convertase degrading agent is characterized by comprising the following steps:

9. A seed dressing method of a plant organic convertase degradation agent is characterized by comprising the following steps:

step 1: diluting, namely diluting the plant organic converting enzyme degradation agent and water according to the ratio of 1: 100-300;

step 2: mixing seeds, namely uniformly spraying the diluted plant organic converting enzyme degradation agent on the surfaces of the seeds, fully stirring the seeds to ensure that the surfaces of the seeds are uniformly covered with the plant organic converting enzyme degradation agent, and tightly covering the stirred seeds for 3-4 h; and 4, step 4: and (6) sowing.

10. A root irrigation method of a plant organic convertase degradation agent is characterized by comprising the following steps: