CN110810285A - Device and method for directional mating of small yellow croakers - Google Patents
Device and method for directional mating of small yellow croakers Download PDFInfo
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- CN110810285A CN110810285A CN201911250948.1A CN201911250948A CN110810285A CN 110810285 A CN110810285 A CN 110810285A CN 201911250948 A CN201911250948 A CN 201911250948A CN 110810285 A CN110810285 A CN 110810285A
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- yellow croakers
- directional mating
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- 230000013011 mating Effects 0.000 title claims abstract description 60
- 241001596950 Larimichthys crocea Species 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 239000003814 drug Substances 0.000 claims abstract description 19
- 235000013601 eggs Nutrition 0.000 claims abstract description 15
- 238000005192 partition Methods 0.000 claims abstract description 9
- 241000251468 Actinopterygii Species 0.000 claims description 51
- 239000013535 sea water Substances 0.000 claims description 32
- 230000002196 ecbolic effect Effects 0.000 claims description 9
- 230000001681 protective effect Effects 0.000 claims description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- 238000009287 sand filtration Methods 0.000 claims description 5
- DBPWSSGDRRHUNT-UHFFFAOYSA-N 17alpha-hydroxy progesterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(=O)C)(O)C1(C)CC2 DBPWSSGDRRHUNT-UHFFFAOYSA-N 0.000 claims description 3
- DOMWKUIIPQCAJU-LJHIYBGHSA-N Hydroxyprogesterone caproate Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(C)=O)(OC(=O)CCCCC)[C@@]1(C)CC2 DOMWKUIIPQCAJU-LJHIYBGHSA-N 0.000 claims description 3
- 210000001015 abdomen Anatomy 0.000 claims description 3
- 230000026109 gonad development Effects 0.000 claims description 3
- 239000003688 hormone derivative Substances 0.000 claims description 3
- 229950000801 hydroxyprogesterone caproate Drugs 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 238000010276 construction Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 229940079593 drug Drugs 0.000 abstract description 2
- 239000004576 sand Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 5
- 238000005286 illumination Methods 0.000 description 4
- 230000009027 insemination Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 241000356842 Larimichthys polyactis Species 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a device and a method for directional mating of small yellow croakers, which are characterized in that: the medicine box is characterized by comprising a main body, wherein a detachable partition plate is arranged in the middle of the main body, the partition plate divides the main body into two parts, a water inlet is formed in the side wall of one part, a water outlet is formed in the bottom of the other part, a collecting box is arranged on the side wall, close to the water outlet, of the main body, and a medicine box used for placing medicines is further arranged on the main body. By the device and the method for directional mating of the small yellow croakers, fertilized eggs of offspring of the small yellow croakers subjected to directional mating by male and female pairs 1 can be obtained simply and conveniently, the rapid construction of the small yellow croaker holomorphic family is realized, a foundation is laid for the breeding of the small yellow croaker family, and meanwhile, the labor cost and the material cost are greatly saved.
Description
Technical Field
The invention belongs to the field of breeding of marine fishes in aquaculture, and particularly relates to a device and a method for directional mating of small yellow croakers.
Background
The little yellow croaker is small in sex courage, the success rate of obtaining fertilized eggs by carrying out 1-to-1 natural mating is very low and less than 10 percent under the traditional seawater seedling breeding facility and environmental conditions, and the artificial insemination method has the problems of large workload, low fertilization rate, high death rate of parent fish and the like, and the development of the little yellow croaker family breeding work is seriously influenced.
Disclosure of Invention
In order to solve the technical problems, the invention adopts the following scheme:
a device for directional mating of small yellow croakers is characterized in that: the medicine box is characterized by comprising a main body, wherein a detachable partition plate is arranged in the middle of the main body, the partition plate divides the main body into two parts, a water outlet is formed in the bottom of one part of the side wall, which is provided with a water inlet, of the other part of the side wall, a collecting box is arranged on the side wall, which is close to the water outlet, of the main body, and a medicine box for placing medicines is further arranged on the main body.
The yellow croaker directional mating device is characterized in that: the main part is provided with a protective cover, the protective cover is provided with a clamping groove, and the partition plate is clamped in the clamping groove.
The yellow croaker directional mating device is characterized in that: an air charging device is arranged in the protective cover and arranged on the locking main body.
The yellow croaker directional mating device is characterized in that: the medicine box is arranged in the protective cover.
The yellow croaker directional mating device is characterized in that: the collection box includes water-collecting box and filter screen, the filter screen sets up the top of water-collecting box.
A method for directional mating of small yellow croakers is characterized in that: the method comprises the following steps:
1) installing above-mentioned yellow croaker directional mating device, will the device level is placed, adds sand filtration sea water to the device and just overflows to the collection box normal water of embryonated egg, opens aerating device.
2) Selecting 1 fish of male and female parent fish with good gonad development, injecting oxytocic LRH-A2, placing the female fish at one side with water inlet, and placing the male fish at one side with water outlet.
3) A mesh bag containing synthetic hormone 17 α -hydroxyprogesterone is hung in the medicine box, and the dosage is 200 ug.
4) After injecting oxytocic for 12 hours, the sand-filtered seawater in the device is changed with water amount of at least 50 percent, and the middle partition plate is pulled open to ensure that the small yellow croakers smoothly enter the mating and spawning stage.
5) Within 24-36 hours of injecting the oxytocic, irradiating with a 15w LED flashlight every 2 hours, checking the spawning condition of the parent fish, after 36 hours, removing the parent fish after spawning of the parent fish is finished, opening a water inlet, and collecting high-quality fertilized eggs on the upper layer into a collection box by micro-flow water.
6) And fishing out fertilized eggs by using a 60-mesh silk net for subsequent incubation and family cultivation.
The method for directional mating of the small yellow croakers is characterized in that: in the step 1), the water temperature of the sand-filtered seawater is maintained at 18 ℃, the dissolved oxygen of the sand-filtered seawater is at least 5mg/L, and the salinity of the sand-filtered seawater is 25.
The method for directional mating of the small yellow croakers is characterized in that: the step 1) also comprises controlling the illuminance and the noise around the yellow croaker directional mating device, so that the illuminance is less than or equal to 500 lux, and the peripheral noise is less than or equal to 50 decibels.
The method for directional mating of the small yellow croakers is characterized in that: the weight of the parent fish in the step 2) is more than 50g, wherein the abdomen of the female fish is soft and slightly raised, and a small amount of semen can flow out by lightly pressing the fingers of the male fish.
The method for directional mating of the small yellow croakers is characterized in that: the injection amount of LRH-A2 was: female fish 5ug/kg fish weight, male fish 2.5ug/kg fish weight.
The device and the method for directionally mating the small yellow croakers have the following beneficial effects:
by the device and the method for directional mating of the small yellow croakers, fertilized eggs of offspring of the small yellow croakers subjected to directional mating by male and female pairs 1 can be obtained simply and conveniently, the rapid construction of the small yellow croaker holomorphic family is realized, a foundation is laid for the breeding of the small yellow croaker family, and meanwhile, the labor cost and the material cost are greatly saved.
Drawings
FIG. 1: the structure of the device for the oriented mating of the small yellow croakers is shown schematically;
FIG. 2: the invention discloses a structural schematic diagram of a device for directional traffic of small yellow croakers.
Description of reference numerals:
1, a water inlet; 2, a protective cover; 3, a partition board; 4, an air charging device; 5, a clamping groove; 6 a medicine box; 7 a water outlet; 8, a collection box; 81, filtering a screen; 82 a water collection box; 9 a main body.
Detailed Description
The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings, so that the technical solutions of the present invention can be understood and appreciated more easily.
With reference to fig. 1 and 2, a device for directional mating of small yellow croakers comprises a main body 9, wherein a water inlet 1 is formed in one side of the main body 9, a collecting box 8 is formed in the other side of the main body 9, and the collecting box 8 is used for collecting fertilized eggs of the small yellow croakers after mating.
Two air charging devices 4 are arranged in the main body 1, a protective cover 2 is arranged on the outer side of each air charging device 4, each protective cover 2 is of a circular arc-shaped structure, and a plurality of small holes are formed in each protective cover 2. The outside of protection casing 2 is provided with draw-in groove 5, and 3 joints of baffle are in order to separate the cavity of main part 1 for two parts in draw-in groove 5, are provided with a plurality of apertures on the baffle 3. Wherein the water inlet 1 is provided on a side wall of a first portion of the main body 1 and the water outlet 7 is provided at a bottom of a second portion of the main body 1.
Be provided with medicine box 6 on the lateral wall of being wrapped up by protection casing 2 on the main part 1, medicine box 6 is used for placing or hangs the medicine, and 6 immersions of medicine box are aquatic, and the medicine diffuses in aqueous gradually.
Collect box 8 and include water collection box 82 and filter screen 81, filter screen 81 sets up in the top of water collection box 82, and filter screen 81 and the last frame laminating of water collection box 82 promptly, and the water in the water collection box 81 overflows and can contact filter screen 81.
A method for directional mating of small yellow croakers by using the device comprises the following steps:
1) the device is installed and then horizontally placed, sand-filtered seawater is added into the device until water in the collection box 8 of the fertilized eggs just overflows, and the air charging device 4 is opened. Wherein the water temperature of the sand filtration seawater is maintained at 18 ℃, the dissolved oxygen of the sand filtration seawater is at least 5mg/L, and the salinity of the sand filtration seawater is 25. Then controlling the illuminance and noise around the yellow croaker directional mating device to make the illuminance not more than 500 luxes and the surrounding noise not more than 50 decibels.
2) 1 fish of male and female parent with good gonad development is selected, the weight is more than 50g, the abdomen of the female fish is soft and slightly raised, and a small amount of seminal fluid can flow out by lightly pressing the fingers of the male fish. After injecting the oxytocic LRH-A2, female fish were placed on the side with the water inlet 1, and male fish were placed on the side with the water outlet 7. The LRH-A2 injection dose is: female fish 5ug/kg fish weight, male fish 2.5ug/kg fish weight.
3) A mesh bag containing synthetic hormone 17 α -hydroxyprogesterone is hung in the medicine box 6, and the dosage is 200 ug.
4) After injecting oxytocic for 12 hours, the sand-filtered seawater in the device is changed with water amount of at least 50 percent, and the middle partition plate 3 is pulled open to ensure that the small yellow croakers smoothly enter the mating and spawning stage.
5) Within 24-36 hours of injecting the oxytocic, irradiating with a 15w LED flashlight every 2 hours, checking the spawning condition of the parent fish, generally, after 36 hours, after the parent fish completes spawning, moving out the parent fish, opening the water inlet 1, collecting high-quality fertilized eggs on the upper layer into the collection box 8 by microflow water.
And fishing out fertilized eggs by using a 60-mesh silk net for subsequent incubation and family cultivation.
Example 1: in a mating pond of a device for directional mating of 25 small yellow croakers, sand-filtered seawater is added into the device, and 25 pairs of parent fish are tested by a method for directional mating of the small yellow croakers, wherein the water temperature of the sand-filtered seawater is maintained at 18 ℃, the dissolved oxygen of the sand-filtered seawater is 5mg/L, the salinity of the sand-filtered seawater is 25, the illumination of a mating environment is controlled to be 500 lux, and the silence is kept during the test.
Comparative example 1: in the device for directional mating of 25 small yellow croakers, sand-filtered seawater is added into the device, and the 1-to-1 natural mating of 25 pairs of parent fish is carried out in a mating pool, wherein the water temperature of the sand-filtered seawater is maintained at 18 ℃, the dissolved oxygen of the sand-filtered seawater is 5mg/L, the salinity of the sand-filtered seawater is 25, the illumination intensity of a mating environment is controlled to be 500 lux, and the silence is kept during the experiment.
Comparative example 2: in a device for directional mating of 25 small yellow croakers, sand filtered seawater is added into the device, and 25 artificial sperm-egg extrusion insemination is carried out on parent fish in a mating pool, wherein the water temperature of the sand filtered seawater is maintained at 18 ℃, the dissolved oxygen of the sand filtered seawater is 5mg/L, the salinity of the sand filtered seawater is 25, the illumination of a mating environment is controlled to be 500 luxes, and the silence is kept during the experiment.
Comparative example 3: in 25 200L barrels, sand filtered seawater is added into the barrels, and 1-to-1 natural mating is carried out on 25 pairs of parent fishes in the barrels, wherein the water temperature of the sand filtered seawater is maintained at 18 ℃, the dissolved oxygen of the sand filtered seawater is 5mg/L, the salinity of the sand filtered seawater is 25, the illumination of the mating environment is controlled to be 500 luxes, and the rest is kept during the experiment.
The results of the experiments are shown in the following table:
the results of the above experiments show that the natural mating method has a low fertilization success rate although the parent fish is alive. The artificial insemination method by squeezing sperm and eggs has the advantages that although the success rate of insemination is good, the hatching rate is low, the mortality rate of female fishes in parent fishes is extremely high, and male fishes partially die. By the device and the method, the success rate of fertilization of the small yellow croakers is high, the hatching rate is high, the parent fish cannot die, and the device and the method have remarkable advantages compared with the conventional mating method.
By the device and the method for directional mating of the small yellow croakers, fertilized eggs of offspring of the small yellow croakers subjected to directional mating by male and female pairs 1 can be obtained simply and conveniently, the rapid construction of the full sib families of the small yellow croakers is realized, a foundation is laid for the breeding of the small yellow croakers, and meanwhile, the labor cost and the material cost are greatly saved.
Claims (10)
1. A device for directional mating of small yellow croakers is characterized in that: including main part (9), be provided with detachable baffle (3) in the middle of main part (9), baffle (3) will main part (1) is separated for two parts, and one of them partial lateral wall is provided with water inlet (1), and the bottom of another part is provided with outlet (7), being close to of main part (9) be provided with on the lateral wall of outlet (7) and collect box (8), still be provided with medicine box (6) that are used for placing the medicine on main part (1).
2. Apparatus for the directional mating of small yellow croakers according to claim 1, wherein: be provided with safety cover (2) on main part (1), be provided with draw-in groove (5) on safety cover (2), baffle (3) joint is in draw-in groove (5).
3. Apparatus for the directional mating of yellow croakers according to claim 2, wherein: be provided with aerating device (4) in safety cover (2), aerating device (4) set up on pinning main part (1).
4. Apparatus for the directional mating of yellow croakers according to claim 2, wherein: the medicine box (6) is arranged in the protective cover (2).
5. Apparatus for the directional mating of small yellow croakers according to claim 1, wherein: collect box (8) including catchment box (82) and filter screen (81), filter screen (81) set up the top of catchment box (82).
6. A method for directional mating of small yellow croakers using the apparatus for directional mating of small yellow croakers according to any one of claims 1 to 5, characterized in that: the method comprises the following steps:
1) installing above-mentioned yellow croaker directional mating device, will the device level is placed, adds sand filtration sea water to the device and just overflows to the collection box normal water of embryonated egg, opens aerating device.
2) Selecting 1 fish of male and female parent fish with good gonad development, injecting oxytocic LRH-A2, placing the female fish at one side with water inlet, and placing the male fish at one side with water outlet.
3) A mesh bag containing synthetic hormone 17 α -hydroxyprogesterone is hung in the medicine box, and the dosage is 200 ug.
4) After injecting oxytocic for 12 hours, the sand-filtered seawater in the device is changed with water amount of at least 50 percent, and the middle partition plate is pulled open to ensure that the small yellow croakers smoothly enter the mating and spawning stage.
5) Within 24-36 hours of injecting the oxytocic, irradiating with a 15w LED flashlight every 2 hours, checking the spawning condition of the parent fish, after 36 hours, removing the parent fish after spawning of the parent fish is finished, opening a water inlet, and collecting high-quality fertilized eggs on the upper layer into a collection box by micro-flow water.
6) And fishing out fertilized eggs by using a 60-mesh silk net for subsequent incubation and family cultivation.
7. Method for the directional mating of small yellow croakers according to claim 6, wherein: in the step 1), the water temperature of the sand-filtered seawater is maintained at 18 ℃, the dissolved oxygen of the sand-filtered seawater is at least 5mg/L, and the salinity of the sand-filtered seawater is 25.
8. Method for the directional mating of small yellow croakers according to claim 6, wherein: the step 1) also comprises controlling the illuminance and the noise around the yellow croaker directional mating device, so that the illuminance is less than or equal to 500 lux, and the peripheral noise is less than or equal to 50 decibels.
9. Method for the directional mating of small yellow croakers according to claim 6, wherein: the weight of the parent fish in the step 2) is more than 50g, wherein the abdomen of the female fish is soft and slightly raised, and a small amount of semen can flow out by lightly pressing the fingers of the male fish.
10. Method for the directional mating of small yellow croakers according to claim 6, wherein: the injection amount of LRH-A2 was: female fish 5ug/kg fish weight, male fish 2.5ug/kg fish weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201911250948.1A CN110810285A (en) | 2019-12-09 | 2019-12-09 | Device and method for directional mating of small yellow croakers |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911250948.1A CN110810285A (en) | 2019-12-09 | 2019-12-09 | Device and method for directional mating of small yellow croakers |
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| CN110810285A true CN110810285A (en) | 2020-02-21 |
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| CN201911250948.1A Pending CN110810285A (en) | 2019-12-09 | 2019-12-09 | Device and method for directional mating of small yellow croakers |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897308A (en) * | 2010-07-29 | 2010-12-01 | 中国水产科学研究院黄海水产研究所 | Equipment and method for collecting industrially cultivated pelagic eggs |
| CN104904636A (en) * | 2015-06-10 | 2015-09-16 | 浙江省海洋水产研究所 | Method for artificially-induced spawning of little yellow croaker |
| EP3275311A1 (en) * | 2015-03-26 | 2018-01-31 | Nippon Suisan Kaisha, Ltd. | Method of maintaining ovaries of marine fish, method of adjusting culture medium, and method of producing eggs or fertilized eggs of marine fish |
| CN107897052A (en) * | 2017-09-05 | 2018-04-13 | 龙泉市富溪农业发展有限公司 | A kind of grouper artificial breeding germplasm method for improving and its device |
| CN108142328A (en) * | 2018-01-11 | 2018-06-12 | 浙江省海洋水产研究所 | A kind of morning of little yellow croaker is numerous and fast seedling-cultivating method |
| CN207531663U (en) * | 2017-12-07 | 2018-06-26 | 浙江师范大学 | Fish oosperm collection device |
-
2019
- 2019-12-09 CN CN201911250948.1A patent/CN110810285A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897308A (en) * | 2010-07-29 | 2010-12-01 | 中国水产科学研究院黄海水产研究所 | Equipment and method for collecting industrially cultivated pelagic eggs |
| EP3275311A1 (en) * | 2015-03-26 | 2018-01-31 | Nippon Suisan Kaisha, Ltd. | Method of maintaining ovaries of marine fish, method of adjusting culture medium, and method of producing eggs or fertilized eggs of marine fish |
| CN104904636A (en) * | 2015-06-10 | 2015-09-16 | 浙江省海洋水产研究所 | Method for artificially-induced spawning of little yellow croaker |
| CN107897052A (en) * | 2017-09-05 | 2018-04-13 | 龙泉市富溪农业发展有限公司 | A kind of grouper artificial breeding germplasm method for improving and its device |
| CN207531663U (en) * | 2017-12-07 | 2018-06-26 | 浙江师范大学 | Fish oosperm collection device |
| CN108142328A (en) * | 2018-01-11 | 2018-06-12 | 浙江省海洋水产研究所 | A kind of morning of little yellow croaker is numerous and fast seedling-cultivating method |
Non-Patent Citations (1)
| Title |
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Application publication date: 20200221 |