CN110760442A - Carbon dioxide cell culture case - Google Patents
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The utility model provides a carbon dioxide cell culture case, carbon dioxide admission line and inner loop supply air duct are drawn forth at the top of interior casing, and inner loop suction air duct is drawn forth to the bottom of interior casing, and the other atomizing humidifier that is equipped with on the inner loop suction air duct to be connected to inner loop supply air duct through heating line and heat dissipation pipeline, be equipped with air cleaner and fan on the inner loop supply air duct in proper order, and the other fresh air pipeline that is equipped with on the inner loop supply air duct, be equipped with the fourth solenoid valve on the fresh air duct. The invention uses the pipeline to make the air in the incubator circularly flow, the air draft and the air supply are soft after being processed by the air homogenizing plate, the cell culture is not influenced, the heating, the humidifying, the heat dissipation, the fresh air supplement, the filtration and other processing are additionally arranged on the circulating pipeline, the concentration, the temperature and the humidity of the carbon dioxide in the incubator are all controlled in a closed loop way, the intelligence is high, the automatic adjustment can be realized, the manual regular inspection and adjustment are not needed, the growth environment of the cells in the incubator can be maintained, and the invention is very beneficial to the growth of the cells.
Description
Technical Field
The invention belongs to the technical field of biological experiment equipment, and particularly relates to a carbon dioxide cell incubator.
Background
In recent years, with the progress of science and technology, the development of biotechnology has become more mature. In the field of biotechnology, cell culture plays an irreplaceable role in the research fields of cell biology and molecular biology. The carbon dioxide cell culture box is a common instrument for cell tissue culture, simulates the growth environment of cell tissues in organisms in the culture box, and ensures that the concentration, temperature and humidity of carbon dioxide in the culture box meet the growth requirements of cells, so that the cell tissues are cultured, and then corresponding cell tissue products are obtained. The incubator is required to have stable temperature (37 ℃), stable CO2 level (5%), high relative saturation humidity (95%), and the success or failure of the culture results directly influences the success or failure of subsequent experiments.
In the prior art, most carbon dioxide cell incubators are inconvenient to control humidity, temperature and carbon dioxide, need manual regular inspection, adjustment, and the intellectuality is not high, seriously influences the growth environment of cells in the incubator, and is very unfavorable to the growth of cells.
Disclosure of Invention
The invention mainly solves the technical problems in the prior art and provides a carbon dioxide cell incubator.
The technical problem of the invention is mainly solved by the following technical scheme: a carbon dioxide cell incubator comprises an inner shell, an outer shell and a heat insulation layer between the inner shell and the outer shell, wherein an electric auxiliary heating device is arranged on the outer surface of the inner shell, a carbon dioxide concentration sensor, a temperature sensor and a humidity sensor are arranged in a cavity of the inner shell, a carbon dioxide air inlet pipeline and an internal circulation air supply pipeline are led out of the top of the inner shell, and an internal circulation air exhaust pipeline is led out of the bottom of the inner shell; the carbon dioxide gas inlet pipeline is sequentially provided with a first electromagnetic valve and a pressure reducing valve and communicated to the carbon dioxide tank, and a pressure gauge is arranged beside the carbon dioxide gas inlet pipeline behind the pressure reducing valve; an atomization humidifier is arranged beside the internal circulation air exhaust pipeline and is connected to the internal circulation air supply pipeline through a heating pipeline and a heat dissipation pipeline, a heater and a second electromagnetic valve are arranged on the heating pipeline, a radiator and a third electromagnetic valve are arranged on the heat dissipation pipeline, an air filter and a fan are sequentially arranged on the internal circulation air supply pipeline, a fresh air pipeline is arranged beside the internal circulation air supply pipeline in front of the air filter, and a fourth electromagnetic valve is arranged on the fresh air pipeline; the electric auxiliary heating device, the carbon dioxide concentration sensor, the temperature sensor, the humidity sensor, the atomization humidifier, the heater, the radiator, the air filter, the fan, the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve and the fourth electromagnetic valve are electrically connected to the controller.
Preferably, an upper air homogenizing plate is arranged on the inner side of the top of the inner shell, a lower air homogenizing plate is arranged on the inner side of the bottom of the inner shell, inner ports of the carbon dioxide air inlet pipeline and the inner circulating air supply pipeline are arranged on the upper air homogenizing plate, and an inner port of the inner circulating air suction pipeline is arranged below the lower air homogenizing plate.
Preferably, the internal circulation air draft pipeline is further provided with an air draft rectifying device, the internal circulation air supply pipeline is further provided with an air supply rectifying device, and the air supply rectifying device is arranged behind the fan.
The using method of the carbon dioxide cell incubator comprises the following steps: initially, the controller controls the third electromagnetic valve, the fourth electromagnetic valve and the radiator to be closed, the first electromagnetic valve, the electric auxiliary heating device, the second electromagnetic valve, the heater, the fan and the atomization humidifier to be opened, air in the temperature incubator circularly flows and is heated while the incubator is heated, carbon dioxide gas enters the incubator through the pressure reducing valve, the carbon dioxide concentration sensor, the temperature sensor and the humidity sensor feed monitoring data back to the controller in real time, the controller controls the first electromagnetic valve, the heater and the atomization humidifier to be closed in due time after the carbon dioxide concentration, the temperature and the humidity respectively reach set values, and at the moment, a vessel loaded with a separated cell sample is placed into the incubator to start cell culture in the superclean bench;
in the cell culture process, a carbon dioxide concentration sensor monitors the concentration of carbon dioxide in the incubator in real time, if the concentration of the carbon dioxide is too low, the controller timely opens the first electromagnetic valve to supplement the carbon dioxide into the incubator, if the concentration of the carbon dioxide is too high, the controller timely opens the fourth electromagnetic valve, and purified fresh air enters the internal circulation air supply pipeline through the fresh air pipeline to dilute the carbon dioxide in the incubator;
in the cell culture process, the temperature sensor monitors the temperature in the incubator in real time, if the temperature is too low, the controller timely opens the heater and the second electromagnetic valve to heat the air in the incubator, and if the temperature is too high, the controller timely opens the radiator and the third electromagnetic valve to dissipate the heat of the air in the incubator;
in the cell culture process, humidity sensor real-time supervision incubator in, if humidity is low excessively, the atomizing humidifier is opened in good time to the controller, improves the humidity in the incubator, if humidity is too high, the atomizing humidifier is closed in good time to the controller, and the humidity in the incubator is reduced through heater heating or appropriate amount supplementary new trend.
The invention uses the pipeline to make the air in the incubator circularly flow, the air draft and the air supply are soft after being processed by the air homogenizing plate, the cell culture is not influenced, the heating, humidifying, heat dissipation, fresh air supplement, filtration and other processing are additionally arranged on the circulating pipeline, the concentration, the temperature and the humidity of the carbon dioxide in the incubator are controlled in a closed loop mode, the intelligentization is high, the automatic adjustment can be realized, the manual regular inspection and adjustment are not needed, the growth environment of the cells in the incubator can be maintained, and the invention is very beneficial to the growth of the cells.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention.
In the figure, 1-an inner shell, 2-an outer shell, 3-a heat insulation layer, 4-an electric auxiliary heating device, 5-an upper air homogenizing plate, 6-a lower air homogenizing plate, 7-a temperature sensor, 8-a humidity sensor, 9-a carbon dioxide tank, 10-a pressure reducing valve, 11-a first electromagnetic valve, 12-a carbon dioxide inlet pipeline, 13-a pressure gauge, 14-an internal circulation air suction pipeline, 15-an air suction rectifying device, 16-a heating pipeline, 17-a heater, 18-a second electromagnetic valve, 19-a heat dissipation pipeline, 20-a radiator, 21-a third electromagnetic valve, 22-an air filter, 23-a fan, 24-an internal circulation air supply pipeline, 25-an air supply rectifying device, 26-a fresh air pipeline and 27-a fourth electromagnetic valve, 28-atomizing humidifier, 29-carbon dioxide concentration sensor.
Detailed Description
The technical scheme of the invention is further specifically described by the following embodiments and the accompanying drawings.
Example (b): referring to fig. 1, a carbon dioxide cell incubator comprises an inner shell, an outer shell and a heat insulation layer between the inner shell and the outer shell, wherein an electric auxiliary heating device is arranged on the outer surface of the inner shell, a carbon dioxide concentration sensor, a temperature sensor and a humidity sensor are arranged in a cavity of the inner shell, a carbon dioxide inlet pipeline and an internal circulation air supply pipeline are led out from the top of the inner shell, and an internal circulation air exhaust pipeline is led out from the bottom of the inner shell; the carbon dioxide gas inlet pipeline is sequentially provided with a first electromagnetic valve and a pressure reducing valve and communicated to the carbon dioxide tank, and a pressure gauge is arranged beside the carbon dioxide gas inlet pipeline behind the pressure reducing valve; an atomization humidifier is arranged beside the internal circulation air exhaust pipeline and is connected to the internal circulation air supply pipeline through a heating pipeline and a heat dissipation pipeline, a heater and a second electromagnetic valve are arranged on the heating pipeline, a radiator and a third electromagnetic valve are arranged on the heat dissipation pipeline, an air filter and a fan are sequentially arranged on the internal circulation air supply pipeline, a fresh air pipeline is arranged beside the internal circulation air supply pipeline in front of the air filter, and a fourth electromagnetic valve is arranged on the fresh air pipeline; the electric auxiliary heating device, the carbon dioxide concentration sensor, the temperature sensor, the humidity sensor, the atomization humidifier, the heater, the radiator, the air filter, the fan, the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve and the fourth electromagnetic valve are electrically connected to the controller. Preferably, an upper air homogenizing plate is arranged on the inner side of the top of the inner shell, a lower air homogenizing plate is arranged on the inner side of the bottom of the inner shell, inner ports of the carbon dioxide air inlet pipeline and the internal circulation air supply pipeline are arranged on the upper air homogenizing plate, and an inner port of the internal circulation air exhaust pipeline is arranged below the lower air homogenizing plate; the internal circulation air draft pipeline is also provided with an air draft rectifying device, the internal circulation air supply pipeline is also provided with an air supply rectifying device, and the air supply rectifying device is arranged behind the fan.
The using method of the carbon dioxide cell incubator comprises the following steps: initially, the controller controls the third electromagnetic valve, the fourth electromagnetic valve and the radiator to be closed, the first electromagnetic valve, the electric auxiliary heating device, the second electromagnetic valve, the heater, the fan and the atomization humidifier to be opened, air in the temperature incubator circularly flows and is heated while the incubator is heated, carbon dioxide gas enters the incubator through the pressure reducing valve, the carbon dioxide concentration sensor, the temperature sensor and the humidity sensor feed monitoring data back to the controller in real time, the controller controls the first electromagnetic valve, the heater and the atomization humidifier to be closed in due time after the carbon dioxide concentration, the temperature and the humidity respectively reach set values, and at the moment, a vessel loaded with a separated cell sample is placed into the incubator to start cell culture in the superclean bench; in the cell culture process, a carbon dioxide concentration sensor monitors the concentration of carbon dioxide in the incubator in real time, if the concentration of the carbon dioxide is too low, the controller timely opens the first electromagnetic valve to supplement the carbon dioxide into the incubator, if the concentration of the carbon dioxide is too high, the controller timely opens the fourth electromagnetic valve, and purified fresh air enters the internal circulation air supply pipeline through the fresh air pipeline to dilute the carbon dioxide in the incubator; in the cell culture process, the temperature sensor monitors the temperature in the incubator in real time, if the temperature is too low, the controller timely opens the heater and the second electromagnetic valve to heat the air in the incubator, and if the temperature is too high, the controller timely opens the radiator and the third electromagnetic valve to dissipate the heat of the air in the incubator; in the cell culture process, humidity sensor real-time supervision incubator in, if humidity is low excessively, the atomizing humidifier is opened in good time to the controller, improves the humidity in the incubator, if humidity is too high, the atomizing humidifier is closed in good time to the controller, and the humidity in the incubator is reduced through heater heating or appropriate amount supplementary new trend.
The invention uses the pipeline to make the air in the incubator circularly flow, the air draft and the air supply are soft after being processed by the air homogenizing plate, the cell culture is not influenced, the heating, humidifying, heat dissipation, fresh air supplement, filtration and other processing are additionally arranged on the circulating pipeline, the concentration, the temperature and the humidity of the carbon dioxide in the incubator are controlled in a closed loop mode, the intelligentization is high, the automatic adjustment can be realized, the manual regular inspection and adjustment are not needed, the growth environment of the cells in the incubator can be maintained, and the invention is very beneficial to the growth of the cells.
Finally, it should be noted that the above embodiments are merely representative examples of the present invention. It is obvious that the invention is not limited to the above-described embodiments, but that many variations are possible. Any simple modification, equivalent change and modification made to the above embodiments in accordance with the technical spirit of the present invention should be considered to be within the scope of the present invention.
Claims (4)
1. A carbon dioxide cell incubator comprises an inner shell, an outer shell and a heat insulation layer between the inner shell and the outer shell, and is characterized in that an electric auxiliary heating device is arranged on the outer surface of the inner shell, a carbon dioxide concentration sensor, a temperature sensor and a humidity sensor are arranged in a cavity of the inner shell, a carbon dioxide air inlet pipeline and an internal circulation air supply pipeline are led out of the top of the inner shell, and an internal circulation air exhaust pipeline is led out of the bottom of the inner shell; the carbon dioxide gas inlet pipeline is sequentially provided with a first electromagnetic valve and a pressure reducing valve and communicated to the carbon dioxide tank, and a pressure gauge is arranged beside the carbon dioxide gas inlet pipeline behind the pressure reducing valve; an atomization humidifier is arranged beside the internal circulation air exhaust pipeline and is connected to the internal circulation air supply pipeline through a heating pipeline and a heat dissipation pipeline, a heater and a second electromagnetic valve are arranged on the heating pipeline, a radiator and a third electromagnetic valve are arranged on the heat dissipation pipeline, an air filter and a fan are sequentially arranged on the internal circulation air supply pipeline, a fresh air pipeline is arranged beside the internal circulation air supply pipeline in front of the air filter, and a fourth electromagnetic valve is arranged on the fresh air pipeline; the electric auxiliary heating device, the carbon dioxide concentration sensor, the temperature sensor, the humidity sensor, the atomization humidifier, the heater, the radiator, the air filter, the fan, the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve and the fourth electromagnetic valve are electrically connected to the controller.
2. The carbon dioxide cell incubator according to claim 1, wherein the inner side of the top of the inner housing is provided with an upper air distribution plate, the inner side of the bottom of the inner housing is provided with a lower air distribution plate, the inner ports of the carbon dioxide inlet duct and the inner circulation air supply duct are arranged above the upper air distribution plate, and the inner port of the inner circulation air suction duct is arranged below the lower air distribution plate.
3. The carbon dioxide cell incubator according to claim 1 or 2, wherein the internal circulation air draft pipeline is further provided with an air draft rectifying device, the internal circulation air supply pipeline is further provided with an air supply rectifying device, and the air supply rectifying device is arranged behind the fan.
4. A method of using the carbon dioxide cell incubator according to any one of claims 1 to 3, wherein the method of use comprises: initially, the controller controls the third electromagnetic valve, the fourth electromagnetic valve and the radiator to be closed, the first electromagnetic valve, the electric auxiliary heating device, the second electromagnetic valve, the heater, the fan and the atomization humidifier to be opened, air in the temperature incubator circularly flows and is heated while the incubator is heated, carbon dioxide gas enters the incubator through the pressure reducing valve, the carbon dioxide concentration sensor, the temperature sensor and the humidity sensor feed monitoring data back to the controller in real time, the controller controls the first electromagnetic valve, the heater and the atomization humidifier to be closed in due time after the carbon dioxide concentration, the temperature and the humidity respectively reach set values, and at the moment, a vessel loaded with a separated cell sample is placed into the incubator to start cell culture in the superclean bench;
in the cell culture process, a carbon dioxide concentration sensor monitors the concentration of carbon dioxide in the incubator in real time, if the concentration of the carbon dioxide is too low, the controller timely opens the first electromagnetic valve to supplement the carbon dioxide into the incubator, if the concentration of the carbon dioxide is too high, the controller timely opens the fourth electromagnetic valve, and purified fresh air enters the internal circulation air supply pipeline through the fresh air pipeline to dilute the carbon dioxide in the incubator;
in the cell culture process, the temperature sensor monitors the temperature in the incubator in real time, if the temperature is too low, the controller timely opens the heater and the second electromagnetic valve to heat the air in the incubator, and if the temperature is too high, the controller timely opens the radiator and the third electromagnetic valve to dissipate the heat of the air in the incubator;
in the cell culture process, humidity sensor real-time supervision incubator in, if humidity is low excessively, the atomizing humidifier is opened in good time to the controller, improves the humidity in the incubator, if humidity is too high, the atomizing humidifier is closed in good time to the controller, and the humidity in the incubator is reduced through heater heating or appropriate amount supplementary new trend.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910953299.5A CN110760442A (en) | 2019-10-09 | 2019-10-09 | Carbon dioxide cell culture case |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910953299.5A CN110760442A (en) | 2019-10-09 | 2019-10-09 | Carbon dioxide cell culture case |
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| CN110760442A true CN110760442A (en) | 2020-02-07 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110982697A (en) * | 2020-03-03 | 2020-04-10 | 广州中安基因科技有限公司 | Cell culture device for biotechnology research and development |
| CN111849773A (en) * | 2020-08-11 | 2020-10-30 | 中国工程物理研究院核物理与化学研究所 | Cell tritium water irradiation experimental device |
| CN112592829A (en) * | 2021-01-15 | 2021-04-02 | 上海智城分析仪器制造有限公司 | Double-channel air inlet type air microcirculation operation device of carbon dioxide incubator |
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| KR20100056017A (en) * | 2008-11-19 | 2010-05-27 | 주식회사 지니스 | Incubator for cell culture |
| CN102758270A (en) * | 2012-06-21 | 2012-10-31 | 合肥日新高温技术有限公司 | High-performance carbon fiber pre-oxidation furnace |
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| JP2017077208A (en) * | 2015-10-21 | 2017-04-27 | 株式会社エアレックス | incubator |
| CN109439544A (en) * | 2018-11-07 | 2019-03-08 | 郑州韦尔特生物科技有限公司 | A kind of carbon dioxide cell incubator |
| CN208844101U (en) * | 2018-07-11 | 2019-05-10 | 吉林济惠生物科技有限公司 | A kind of carbon dioxide incubator |
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2019
- 2019-10-09 CN CN201910953299.5A patent/CN110760442A/en active Pending
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|---|---|---|---|---|
| KR20100056017A (en) * | 2008-11-19 | 2010-05-27 | 주식회사 지니스 | Incubator for cell culture |
| CN102758270A (en) * | 2012-06-21 | 2012-10-31 | 合肥日新高温技术有限公司 | High-performance carbon fiber pre-oxidation furnace |
| CN204417513U (en) * | 2014-12-16 | 2015-06-24 | 江西省银丰鼎诚生物工程有限公司 | A kind of can the CO2gas incubator of on-line checkingi |
| JP2017077208A (en) * | 2015-10-21 | 2017-04-27 | 株式会社エアレックス | incubator |
| CN208844101U (en) * | 2018-07-11 | 2019-05-10 | 吉林济惠生物科技有限公司 | A kind of carbon dioxide incubator |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110982697A (en) * | 2020-03-03 | 2020-04-10 | 广州中安基因科技有限公司 | Cell culture device for biotechnology research and development |
| CN111849773A (en) * | 2020-08-11 | 2020-10-30 | 中国工程物理研究院核物理与化学研究所 | Cell tritium water irradiation experimental device |
| CN112592829A (en) * | 2021-01-15 | 2021-04-02 | 上海智城分析仪器制造有限公司 | Double-channel air inlet type air microcirculation operation device of carbon dioxide incubator |
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