CN112592829A - Double-channel air inlet type air microcirculation operation device of carbon dioxide incubator - Google Patents

Double-channel air inlet type air microcirculation operation device of carbon dioxide incubator Download PDF

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Publication number
CN112592829A
CN112592829A CN202110057123.9A CN202110057123A CN112592829A CN 112592829 A CN112592829 A CN 112592829A CN 202110057123 A CN202110057123 A CN 202110057123A CN 112592829 A CN112592829 A CN 112592829A
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carbon dioxide
air
air inlet
channel
liner
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CN202110057123.9A
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沈水兴
张钱
徐非
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Shanghai Zhicheng Analytical Instrument Manufacturing Co ltd
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Shanghai Zhicheng Analytical Instrument Manufacturing Co ltd
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Publication of CN112592829A publication Critical patent/CN112592829A/en
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/24Recirculation of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26Conditioning fluids entering or exiting the reaction vessel
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02Filters
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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Abstract

The invention discloses a double-channel air inlet type air microcirculation operation device of a carbon dioxide incubator, wherein a carbon dioxide double-channel air inlet sub-assembly and an air microcirculation operation sub-assembly are respectively connected with a controller, the carbon dioxide double-channel air inlet sub-assembly comprises a main carbon dioxide air inlet channel and an auxiliary carbon dioxide air inlet channel, when the controller detects that the concentration of carbon dioxide in a liner is lower than a set value, the controller automatically switches to the other carbon dioxide air inlet channel, and continuously conveys carbon dioxide without intervals; the air of the inner container is subjected to double filtration and sent to the bottom of the inner container to reciprocate in a circulating way under the action of the air pump, so that the air microcirculation operation is carried out. The invention has the advantages of continuously conveying carbon dioxide without intervals and effectively preventing air pollution of the inner container.

Description

Double-channel air inlet type air microcirculation operation device of carbon dioxide incubator
Technical Field
The invention relates to the fields of microorganisms, medicine, pharmacy, environmental protection and the like, in particular to a double-channel air inlet type air microcirculation operation device of a carbon dioxide incubator.
Background
The carbon dioxide incubator adopts an artificial method to shape the growth and propagation environment of microorganisms, cells and the like, at present, the traditional artificial method adopted is a fan airflow circulating device, but the inner container of the carbon dioxide incubator is easy to cause air pollution, and the good growth environment of the microorganisms and the cells in the whole process cannot be created, which is the problem to be solved emphatically in the application.
When the carbon dioxide incubator works, the set carbon dioxide concentration is required to be kept, so that the carbon dioxide gas source is insufficient, and the carbon dioxide gas source bottle is required to be replaced in time. At present, the traditional method for replacing the carbon dioxide gas cylinder is manual operation, and the phenomenon that the carbon dioxide gas input is temporarily stopped due to uncertain factors of manual operation errors exists. This is where the present application needs to address.
Disclosure of Invention
The invention aims to provide a double-channel air inlet type air microcirculation operation device of a carbon dioxide incubator, which has reliable performance and can effectively prevent air pollution of an inner container.
In order to solve the technical problems, the invention provides a double-channel air inlet type air microcirculation operation device of a carbon dioxide incubator, which comprises a carbon dioxide double-channel air inlet sub-assembly, an air microcirculation operation sub-assembly and a controller, wherein the carbon dioxide double-channel air inlet sub-assembly and the air microcirculation operation sub-assembly are respectively connected with the controller, and the device comprises:
the carbon dioxide double-channel air inlet sub-assembly comprises a main carbon dioxide air inlet channel and an auxiliary carbon dioxide air inlet channel, wherein each carbon dioxide air inlet channel comprises a carbon dioxide air inlet pipe, a carbon dioxide air source, a pressure reducing valve, a bacterial filter and an electromagnetic valve;
the air microcirculation operation sub-assembly comprises a bacteria filter, an air pump, a high-efficiency filter, a carbon dioxide concentration sensor, at least two liner air outlet pipes and liner air inlet pipes, wherein the liner air outlet pipes are connected to the top of a liner, the liner air inlet pipes are connected to the bottom of the liner, and the liner air outlet pipes are sequentially connected with the bacteria filter, the air pump, the high-efficiency filter, the carbon dioxide concentration sensor and the liner air inlet pipes; under the action of the air pump, the air in the liner sequentially enters the bacterial filter and the high-efficiency filter through the air outlet pipe of the liner for filtration, the concentration of carbon dioxide is monitored by the carbon dioxide concentration sensor, and then the air in the liner is sent to the bottom of the liner for circulation and reciprocation through the air inlet pipe of the liner connected with the three-way joint for air microcirculation operation;
the carbon dioxide concentration sensor detects the concentration of carbon dioxide in the inner container in real time and feeds the concentration of carbon dioxide back to the controller;
and the controller controls the opening and closing of the two electromagnetic valves of the carbon dioxide double-channel air inlet sub-assembly, when the carbon dioxide concentration value of the inner container is lower than a set value, the electromagnetic valve of the current carbon dioxide channel is closed, the electromagnetic valve of the other channel is opened, the carbon dioxide in the inner container is supplemented in time, and the stability of the carbon dioxide concentration is maintained.
The carbon dioxide double-channel air inlet sub-assembly, the air micro-circulation sub-assembly and the controller are arranged on a mounting plate, and the mounting plate is connected with a box body of the carbon dioxide incubator.
And the air of the inner container is subjected to double filtration and sent to the bottom of the inner container to reciprocate circularly under the action of the air pump of the air microcirculation operation sub-assembly, so that the air microcirculation operation is performed.
A temperature sensor is arranged in the middle of the inner container, a humidity sensor is arranged on one side of the inner container, and the temperature and the humidity in the inner container are monitored in real time.
The carbon dioxide concentration sensor is arranged outside the inner container, the carbon dioxide concentration sensor is arranged in an instrument box, and the instrument box is arranged on the mounting plate to monitor the carbon dioxide concentration of the inner container in real time.
The bottom of the inner container is also provided with an evaporation water tray for humidification.
The box cover of the carbon dioxide incubator is provided with a display screen, and the display screen displays the temperature, the humidity and the carbon dioxide concentration in the air of the inner container.
The invention has the following advantages:
1) the carbon dioxide gas continuous conveying device is provided with redundant carbon dioxide conveying channels, can continuously convey carbon dioxide gas without intervals, is reliable in performance and convenient to use, and reduces the labor cost;
2) the invention can accurately control the temperature, the concentration and the humidity of carbon dioxide and effectively prevent the air pollution of the inner container;
3) the air of the inner container is subjected to double filtration under the action of the air pump by the air microcirculation operation sub-assembly, and is sent to the bottom of the inner container to reciprocate in a circulation manner, so that the air microcirculation operation is carried out, and the air pollution of the inner container is effectively prevented;
3) the invention has novel design, unique structure, convenient use and simple maintenance.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the door of the present invention;
description of the figures
1-box cover; 2-a display screen;
3, an inner container; 4-a controller;
5-carbon dioxide connecting pipe A; 6, an instrument box;
7-evaporating water pan; 8-air pump;
9-air outlet pipe A; 10-air inlet pipe A;
11-three way connection M; 12-air outlet pipe B;
13-air inlet pipe B; 14-air connecting pipe;
15-high efficiency filter; 16-CO 2 concentration sensor;
17-solenoid valve a; 18-a main channel;
19-bacterial filter a; 20-secondary channel;
21-pressure reducing valve a; 22-a sheath;
23-air outlet pipe C; 24-carbon dioxide inlet pipe A;
25-carbon dioxide inlet pipe B; 26-carbon dioxide inlet pipe C;
27-air outlet duct D; 28-mounting a plate;
29-air outlet duct E; 30-three way connection N;
31-a box body; 32-a temperature sensor;
33-a humidity sensor; 34-three-way connection a;
35-carbon dioxide connecting pipe B; 36-bacterial filter B;
37-solenoid valve B; 38-pressure relief valve B;
39-bacterial filter C. 40-air outlet pipe F.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Fig. 1 shows a schematic structural diagram of an embodiment of the present invention, and fig. 2 shows a schematic box door of the embodiment of the present invention. As shown in figure 1, the invention provides a double-channel air inlet type air microcirculation operation device of a carbon dioxide incubator, as shown in figure 2, a display screen 2 is arranged on a cover, and the temperature, the humidity and the carbon dioxide concentration of an inner container 3 are displayed on the display screen 2. As shown in fig. 1, the temperature sensor 32 is arranged in the middle of the inner container 3 to monitor the temperature in the inner container 3 in real time; the carbon dioxide concentration sensor 16 is arranged in the instrument box 6 fixed on the mounting plate 28 at the back of the box body 31, monitors the carbon dioxide concentration of the air in the inner container 3 in real time, and simultaneously sends data to the controller; the humidity sensor 33 is disposed at the left portion of the inner container 3, and monitors the humidity in the inner container 3 in real time. The temperature sensor 32, the carbon dioxide concentration sensor 16, and the humidity sensor 33 are electrically connected to the controller 4.
The embodiment of the invention comprises a carbon dioxide double-channel air inlet sub-assembly, an air micro-circulation sub-assembly and a controller 4 which are all arranged on a mounting plate 28 fixedly connected with a box body 31.
According to the carbon dioxide double-channel air inlet sub-assembly in the embodiment of the invention, a controller 4 fixed on a mounting plate 28 applies a signal to open an electromagnetic valve A17, air of a carbon dioxide air source enters a main channel 18, sequentially enters a pressure reducing valve A21, a bacterial filter A19 and an electromagnetic valve A17 from a carbon dioxide air inlet pipe B25, enters a carbon dioxide connecting pipe A5 and a three-way joint A34, and enters the top of an inner container 3 of a carbon dioxide incubator through a carbon dioxide air inlet pipe C26. When the carbon dioxide concentration sensor 16 detects that the concentration of carbon dioxide gas in the air in the liner has reached a set value, it sends a signal to the controller 4, and the controller 4 closes the electromagnetic valve a 17. When the carbon dioxide concentration sensor 16 detects that the concentration of the carbon dioxide in the air of the liner is lower than a set value, a signal is sent to the controller 4, the controller 4 opens the electromagnetic valve A17, and the carbon dioxide enters the liner from the main channel 18 to supplement the concentration of the carbon dioxide.
When the carbon dioxide concentration sensor 16 detects that the concentration of carbon dioxide in the inner container 3 is lower than a set value, a signal is sent to the controller 4, the controller 4 closes the electromagnetic valve A17 of the main channel 18 and simultaneously opens the electromagnetic valve B37 of the secondary channel 20 to automatically switch to the secondary channel 20, and gas of a carbon dioxide gas source enters the pressure reducing valve B38 from the carbon dioxide gas inlet pipe A24, enters the bacterial filter B36, passes through the electromagnetic valve B37, passes through the carbon dioxide connecting pipe B35 and the three-way joint A34, and enters the top of the inner container 3 of the carbon dioxide incubator from the carbon dioxide gas inlet pipe C26. The invention is provided with a double-channel air inlet structure, when the concentration of the carbon dioxide in the inner container is detected to be lower than a set value, the air inlet structure is automatically switched to the other channel, and the carbon dioxide is continuously conveyed without intervals, so that the working efficiency is improved, and the use is convenient.
The air microcirculation operation sub-assembly in the embodiment of the invention is controlled by the controller 4. The air in the carbon dioxide incubator inner container 3 is forced to circulate by the air pump 8. Air is sucked into an air outlet pipe C23 and an air outlet pipe D27 from the top of the inner container 3, the two air outlet pipes C23 and D27 are fixed on a mounting plate 28 through a sheath 22, then pass through a three-way joint M11, an air outlet pipe A9, a bacteria filter C39, an air pump 8, an air outlet pipe A40, an air outlet pipe E29, a high-efficiency filter 15 arranged in an instrument box 6, then pass through an air connecting pipe 14 to a carbon dioxide concentration sensor 16 in the instrument box 6, pass through an air outlet pipe B12 and a three-way joint N30, and finally enter the inner container 3 of the carbon dioxide incubator from the bottom through an air inlet pipe A10 and an air inlet pipe B13.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (5)

1. The utility model provides a formula air microcirculation operation device admits air of binary channels of carbon dioxide incubator which characterized in that: the carbon dioxide binary channels inlet subassembly and the air microcirculation operation subassembly are connected with the controller respectively, wherein:
the carbon dioxide double-channel air inlet sub-assembly comprises a main carbon dioxide air inlet channel and an auxiliary carbon dioxide air inlet channel, wherein each carbon dioxide air inlet channel comprises a carbon dioxide air inlet pipe, a carbon dioxide air source, a pressure reducing valve, a bacterial filter and an electromagnetic valve;
the air microcirculation operation sub-assembly comprises a bacteria filter, an air pump, a high-efficiency filter, a carbon dioxide concentration sensor, at least two liner air outlet pipes and liner air inlet pipes, wherein the liner air outlet pipes are connected to the top of a liner, the liner air inlet pipes are connected to the bottom of the liner, and the liner air outlet pipes are sequentially connected with the bacteria filter, the air pump, the high-efficiency filter, the carbon dioxide concentration sensor and the liner air inlet pipes; under the action of the air pump, the air in the liner sequentially enters the bacterial filter and the high-efficiency filter through the air outlet pipe of the liner for filtration, the concentration of carbon dioxide is monitored by the carbon dioxide concentration sensor, and then the air in the liner is sent to the bottom of the liner for circulation and reciprocation through the air inlet pipe of the liner connected with the three-way joint for air microcirculation operation;
the carbon dioxide concentration sensor detects the concentration of carbon dioxide in the inner container in real time and feeds the concentration of carbon dioxide back to the controller;
and the controller controls the opening and closing of the two electromagnetic valves of the carbon dioxide double-channel air inlet sub-assembly, and when the carbon dioxide concentration value of the inner container is lower than a set value, the electromagnetic valve of the current carbon dioxide channel is closed, and the electromagnetic valve of the other channel is opened.
2. The double-channel air inlet type air microcirculation operation device of the carbon dioxide incubator as claimed in claim 1, wherein: the carbon dioxide double-channel air inlet sub-assembly, the air micro-circulation sub-assembly and the controller are arranged on a mounting plate, and the mounting plate is connected with a box body of the carbon dioxide incubator.
3. The double-channel air inlet type air microcirculation operation device of the carbon dioxide incubator as claimed in claim 1, wherein: a temperature sensor is arranged in the middle of the inner container, a humidity sensor is arranged on one side of the inner container, and the temperature and the humidity in the inner container are monitored in real time.
4. The double-channel air inlet type air microcirculation operation device of the carbon dioxide incubator as claimed in claim 1 or 2, wherein: the carbon dioxide concentration sensor is arranged outside the inner container, the carbon dioxide concentration sensor is arranged in an instrument box, and the instrument box is arranged on the mounting plate.
5. The double-channel air inlet type air microcirculation operation device of the carbon dioxide incubator as claimed in claim 1, wherein: an evaporating water tray is also arranged at the bottom of the inner container.
CN202110057123.9A 2021-01-15 2021-01-15 Double-channel air inlet type air microcirculation operation device of carbon dioxide incubator Withdrawn CN112592829A (en)

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CN202110057123.9A CN112592829A (en) 2021-01-15 2021-01-15 Double-channel air inlet type air microcirculation operation device of carbon dioxide incubator

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Application Number Priority Date Filing Date Title
CN202110057123.9A CN112592829A (en) 2021-01-15 2021-01-15 Double-channel air inlet type air microcirculation operation device of carbon dioxide incubator

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114790422A (en) * 2021-05-08 2022-07-26 上海纹秋环境检测技术有限公司 Carbon dioxide incubator for public place sanitation detection

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JPH0775552A (en) * 1993-09-07 1995-03-20 Sanyo Electric Co Ltd Culture system
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CN109321449A (en) * 2018-10-09 2019-02-12 郑州韦尔特生物科技有限公司 A kind of microbial cultivation device
CN210886029U (en) * 2019-09-27 2020-06-30 广州华塔数码科技有限公司 Microbial cultivation agitating unit
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CN211367567U (en) * 2019-10-18 2020-08-28 浙江泰林医学工程有限公司 Automatic switching device for air source of carbon dioxide incubator

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114790422A (en) * 2021-05-08 2022-07-26 上海纹秋环境检测技术有限公司 Carbon dioxide incubator for public place sanitation detection

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