CN110726849A - Automatic replacement detection device for biological detection chip - Google Patents
Automatic replacement detection device for biological detection chip Download PDFInfo
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- CN110726849A CN110726849A CN201910948911.XA CN201910948911A CN110726849A CN 110726849 A CN110726849 A CN 110726849A CN 201910948911 A CN201910948911 A CN 201910948911A CN 110726849 A CN110726849 A CN 110726849A
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- chip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1011—Control of the position or alignment of the transfer device
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
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- Chemical & Material Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an automatic replacement detection device for a biological detection chip, which comprises: a bottom plate provided with a bracket plate; the motor is arranged above the bottom plate and fixedly connected with the support plate through a first connecting plate, the motor is connected with a rotary table, a mounting groove and a positioning hole for mounting a chip are formed in the rotary table, and the front side of the chip is arranged towards the center direction of the rotary table; the camera is arranged between the bottom plate and the motor and is fixedly connected with the support plate through a second connecting plate; the titrating tube head is arranged between the camera and the bottom plate, the titrating tube head is connected with a titrating pump through a hose, and the other end of the titrating pump is connected with a detection sample storage tank through a hose; the first infrared device is arranged on the bottom plate; the motor, the camera, the titration pump and the positioning device are electrically connected with a micro-controller; the invention has the beneficial effects that: the whole detection process is completed in a full-automatic mode, detection personnel do not need to operate, and the device is simple in structure, accurate in positioning and high in detection efficiency.
Description
Technical Field
The invention relates to the field of automatic detection of biochips, in particular to an automatic replacement detection device for a biological detection chip.
Background
Air is a necessary environment for human beings to live. The microorganisms in the air mainly come from soil, water surface, animals and plants, human body and production activities, sewage and dirt treatment and the like, the composition concentration is unstable, the types are various, bacteria, fungi, viruses, phages and the like exist, and the microorganism particles are important components of air pollutants. Airborne microorganisms exist in aerosol form. Dust, particles or liquid droplets with microorganisms suspended in the air are microbial aerosol, the microbial aerosol can stay in the air for a long time, and certain microorganisms can penetrate pathogenic bacteria into people through fine particles in the air, so that harm is brought to the health of the people.
The biochip technology has become an important means for the rapid detection of microorganisms, and with the rapid development of detection and analysis technology in the field of life science, the biochip technology has the characteristics of high flux, high sensitivity and high specificity, and has a wide application prospect in the detection of microorganisms, and the detection of pathogenic microorganisms in the air requires the air to be condensed into liquid for collection and then detection.
Disclosure of Invention
In order to solve the technical problem of how to realize automatic detection of a detected sample, the present invention provides an automatic replacement detection device for a biological detection chip to solve the above technical problem.
The technical scheme adopted by the invention for solving the technical problem is as follows:
according to one aspect of the present invention, there is provided an automatic replacement testing apparatus for a biological testing chip, comprising:
a base plate on which a bracket plate is disposed;
the motor is arranged above the bottom plate and fixedly connected with the support plate through a first connecting plate, the motor is connected with a rotary table, a mounting groove and a positioning hole for mounting a chip are formed in the rotary table, and the front side of the chip is arranged towards the center direction of the rotary table;
the camera is arranged between the bottom plate and the motor and is fixedly connected with the support plate through a second connecting plate;
the titrating tube head is arranged between the camera and the bottom plate, the titrating tube head is connected with a titrating pump through a hose, and the other end of the titrating pump is connected with a storage tank for storing a detection sample through a hose;
the first infrared device is arranged on the bottom plate;
the motor, the camera, the titration pump, first infrared device electricity is connected with the micro-control machine, the micro-control machine control motor rotates and drives the carousel and rotate the interlock chip and remove, move burette head below when the chip, the signal of first infrared device's transmitting terminal transmission passes the locating hole on the carousel and is received by its receiving terminal, first infrared device feedback information stops to micro-control machine control motor stall, later the micro-control machine control titration pump is drawn and is detected the sample and drip to the chip through burette head, later the real-time picture of taking chip assay change of micro-control machine control camera is uploaded.
Adopt above-mentioned technical scheme, it rotates and cooperates first infrared device to realize the accurate location of chip under the burette head to drive the chip on the carousel through micro-controller control motor to titrate the pump through micro-controller control and realize dripping the detection sample to the chip, and shoot the chip after the chemical examination and upload through the camera, whole testing process complete automation accomplishes, does not need the measurement personnel to go the operation, simple structure moreover, the location is accurate, detection efficiency is higher.
In order to better solve the technical defects, the invention also has a better technical scheme that:
in some embodiments, the automatic airborne microorganism detection apparatus further comprises: a second infrared device for emitting a second infrared light,
the second infrared device is arranged on the bottom plate and electrically connected with the micro-controller, after the camera shoots the tested chip and uploads the chip, the micro-controller controls the motor to rotate to drive the turntable to rotate so as to drive the chip to move, when a gap between the two chips is positioned below the burette head, a signal transmitted by the infrared transmitting end of the second infrared device passes through the positioning hole on the turntable and is received by the infrared receiving end of the positioning hole, the second infrared device feeds back information to the micro-controller to stop rotating, the micro-controller controls the titration pump to empty the detection sample in the storage tank, therefore, the second infrared device can be used for realizing the second accurate positioning of the chip, the titration pump empties the detection sample in the storage tank, and the preparation is made for extracting a new detection sample next time.
In some embodiments, the plurality of mounting grooves are arranged in a circle around the turntable at equal intervals, the plurality of positioning holes are arranged in a circle around the turntable at equal intervals, the circle of positioning holes is arranged on the periphery of the circle of mounting grooves, and each positioning hole is arranged in one-to-one correspondence with each mounting groove.
In some embodiments, the chip is clamped with the mounting groove, thereby facilitating the installation and replacement of the chip.
In some embodiments, the chip clamp is arranged in the mounting groove, two ends of the chip clamp are open, the top of the chip clamp is provided with a through hole, and the chip is clamped with the chip clamp. The clamping mode is adopted for fixing, so that the chip is convenient to install and replace.
In some embodiments, the bottom plate is provided with a sample discharge groove, so that the discharged detection sample can be collected, and the detection sample is prevented from being directly discharged to damage the component.
In some embodiments, the first connecting plate is in an L shape, one side plate of the first connecting plate is used for fixing the motor, and the other side plate is conveniently fixed with the support plate through a bolt, so that the assembly is convenient.
In some embodiments, the air microorganism detection device is provided with a shell on the periphery, the shell is hinged with a door, the shell is arranged to prevent dust particles, microorganisms and the like in the air from polluting the chip, and the door can facilitate the later stage of chip replacement or maintenance.
Drawings
FIG. 1 is a schematic structural view of an automatic replacement testing device for a biological test chip according to an embodiment of the present invention;
FIG. 2 is a right side view of the automatic replacement testing device for biological test chips;
FIG. 3 is a schematic top view of an automatic replacement device for a biological detection chip;
FIG. 4 is a top view of a chip holder of the automatic replacement testing device for a bio-chip.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer and clearer, the present invention is further described in detail below with reference to the accompanying drawings.
As shown in FIGS. 1 to 4, the present invention provides an automatic replacement testing device for a biological test chip, comprising:
the sample collection device comprises a bottom plate 1, wherein a support plate 11 and a sample discharge groove 12 are arranged on the bottom plate 1, and the support plate 11 is fixedly connected with the bottom plate 1;
the motor 2 is arranged above the bottom plate 1, the motor 2 is arranged on the first connecting plate 21, the first connecting plate 21 is fixedly connected with the support plate 11 through bolts, the first connecting plate 21 is L-shaped, an output shaft of the motor 2 is connected with a rotary table 22, the rotary table 22 is provided with mounting grooves and positioning holes 23, a plurality of mounting grooves are equidistantly arranged around the rotary table 22, a plurality of mounting holes 23 are equidistantly arranged around the rotary table 22, a circle of positioning holes 23 are arranged on the periphery of the circle of mounting grooves, each mounting groove is in one-to-one correspondence with each positioning hole 22, chip clamps 24 are arranged in the mounting grooves, two ends of each chip clamp 24 are open, the top of each chip clamp 24 is provided with a through hole 241, and the front surface of;
the camera 3 is arranged between the bottom plate 1 and the motor 2, the camera 3 is arranged on a second connecting plate 31, and the second connecting plate 31 is fixedly connected with the support plate 11;
the titrating tube head 4 is arranged between the camera 3 and the bottom plate 1, the titrating tube head 4 is connected with a titrating pump, the other end of the titrating pump is connected with a storage tank for storing a detection sample through a hose, the titrating tube head 4 is connected with a connecting rod, and the connecting rod is fixedly connected with the support plate 11;
the first infrared device 5 is arranged on the bottom plate 1;
and the second infrared device 6 is arranged on the bottom plate 1 and is positioned at the right side of the first infrared device 5.
The motor 2, the camera 3, the titration pump, the first infrared device 5, the second infrared device 6 electricity is connected with a micro-control machine, the micro-control machine controls the motor 2 to rotate to drive the turntable 22 to rotate to drive the chip 25 to move, when the chip 25 moves to the lower part of the titration tube head 4, the signal transmitted by the transmitting end of the first infrared device 5 passes through the positioning hole 23 on the turntable 22 to be received by the receiving end, the first infrared device 5 feeds back information to the micro-control machine to control the motor 2 to stop rotating, then the micro-control machine controls the titration pump to extract the detection sample in the storage tank and drips onto the chip 25 through the titration tube head 4, then the micro-control machine controls the camera 3 to shoot the picture of the assay change of the chip 25 in real time and upload the picture to a data center, then the micro-control machine controls the motor 2 to continue to rotate to drive the turntable 22 to rotate to drive the chip 25 to move, when the gap between the two chips 25 is positioned below the titration tube head 4, the When receiving, the second infrared device 6 feeds back information to the micro-controller to control the motor 2 to stop rotating, and the micro-controller controls the titration pump to empty the detection sample in the storage tank into the sample discharge tank 12.
In some embodiments, chip 25 snaps directly into the mounting slot, and clip 24 is omitted.
In some embodiments, the airborne microbial detection apparatus is peripherally provided with a housing having a door hinged thereto.
The principle of the automatic replacement detection device of the biological detection chip comprises the following steps: when the micro-controller receives the information of a sample to be detected, the micro-controller controls the motor 2 to rotate to drive the turntable 22 to rotate and link the chip 25 to move, when the chip 25 moves to the position below the burette head 4, the signal transmitted by the transmitting end of the first infrared device 5 passes through the positioning hole 23 on the turntable 22 and is received by the receiving end of the positioning hole, the first infrared device 5 feeds back information to the micro-controller to control the motor 2 to stop rotating, at the moment, the chip 25 is just positioned under the burette head 4, then the micro-controller controls the burette head to pump the detected sample and drips on the chip 25 through the burette head 4, then the micro-controller controls the camera 3 to shoot the picture of the assay change of the chip 25 in real time and upload the picture to the data center, then the micro-controller controls the motor 2 to continuously rotate to drive the turntable 22 to rotate and link the chip 25 to move, when the gap between the two chips 25 is positioned under the burette head 4, the signal transmitted by the When the second infrared device 6 feeds back information to the micro-controller to control the motor 2 to stop rotating, the micro-controller controls the titration pump to empty the detection sample in the storage tank into the sample discharge tank 12, and preparation is made for next detection of the newly collected detection sample. Through the equipment of being connected with data center, can look over the chip picture of shooing, whether have the pathogenic bacterium in judging the detection sample according to the colour change on the chip, whole testing process complete automation accomplishes, does not need the measurement personnel to go the operation, simple structure moreover, and detection efficiency is higher.
The above description is only for the embodiments of the present invention, and it is obvious to those skilled in the art that various changes and modifications can be made without departing from the inventive concept of the present invention, and these changes and modifications are all within the scope of the present invention.
Claims (8)
1. An automatic replacement detection device for a biological detection chip, comprising:
a bottom plate (1) on which a support plate (11) is arranged;
the motor (2) is arranged above the bottom plate (1) and fixedly connected with the support plate (11) through a first connecting plate (21), the motor (2) is connected with a turntable (22), a mounting groove and a positioning hole (23) for mounting a chip (25) are formed in the turntable (22), and the front face of the chip (25) is arranged towards the center direction of the turntable (22);
the camera (3) is arranged between the bottom plate (1) and the motor (2) and is fixedly connected with the support plate (11) through a second connecting plate (31);
the titration tube head (4) is arranged between the camera (3) and the bottom plate (1), the titration tube head (4) is connected with a titration pump through a hose, and the other end of the titration pump is connected with a storage tank for storing a detection sample through a hose;
a first infrared device (5) arranged on the base plate (1);
motor (2), camera (3), titration pump, first infrared device (5) electricity are connected with the micro-control machine, micro-control machine control motor (2) rotate and drive carousel (22) and rotate interlock chip (25) and remove, remove buret head (4) below when chip (25), and the signal of the transmitting terminal transmission of first infrared device (5) passes locating hole (23) on carousel (22) and is received by its receiving terminal and receive the time, first infrared device (5) feedback information arrives micro-control machine control motor (2) stall, and then the micro-control machine control titration pump is drawn and is detected the sample and drip on chip (25) through buret head (4), and later micro-control machine control camera (3) shoot the picture that chip (25) chemical examination changed in real time and upload.
2. The automatic replacement testing device of claim 1, further comprising: second infrared device (6), second infrared device (6) set up on bottom plate (1), second infrared device (6) with the micro-control machine electricity is even, and chip and upload the back after the assay are shot to camera (3), micro-control machine control motor (2) are rotated and are driven carousel (22) and rotate interlock chip (25) and remove, and the gap between two chips (25) is located burette head (4) below, locating hole (23) on the signal of the infrared emission end transmission of second infrared device (6) passes carousel (22) are received by its infrared receiver and are received when, second infrared device (6) feedback information is to micro-control machine control motor (2) stall, the micro-control machine control burette pump carries out the detection sample of evacuation storage tank.
3. The automatic replacement testing device of claim 1 or 2, wherein a plurality of said mounting slots are arranged in a circle around said rotary plate (22) at equal intervals, a plurality of said positioning holes (23) are arranged in a circle around said rotary plate (22) at equal intervals, a circle of said positioning holes (23) is arranged at the periphery of a circle of said mounting slots, and each of said positioning holes (23) is arranged in one-to-one correspondence with each of said mounting slots.
4. The automatic replacement testing device of biological testing chip as claimed in claim 3, wherein said chip (25) is clamped to said mounting groove.
5. The automatic replacement detection device for biological detection chips as claimed in claim 3, wherein a chip clamp (24) is disposed in the mounting groove, two ends of the chip clamp (24) are open, a through hole (241) is disposed at the top of the chip clamp, and the chip (25) is connected with the chip clamp (24) in a clamping manner.
6. The automatic replacement testing device of biological testing chip as claimed in claim 1, wherein said bottom plate (1) is provided with a sample groove (12).
7. The automatic replacement detecting device of biological detecting chip as claimed in claim 1, wherein the first connecting plate (21) is L-shaped.
8. The device as claimed in claim 1, wherein a housing is disposed around the device, and a door is hinged to the housing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910948911.XA CN110726849A (en) | 2019-10-08 | 2019-10-08 | Automatic replacement detection device for biological detection chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910948911.XA CN110726849A (en) | 2019-10-08 | 2019-10-08 | Automatic replacement detection device for biological detection chip |
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| CN110726849A true CN110726849A (en) | 2020-01-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910948911.XA Pending CN110726849A (en) | 2019-10-08 | 2019-10-08 | Automatic replacement detection device for biological detection chip |
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Citations (8)
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| CN105572407A (en) * | 2016-01-27 | 2016-05-11 | 广州万孚生物技术股份有限公司 | Full-automatic fluorescent quantitative immunity analyzer and detection method |
| CN106906137A (en) * | 2017-03-19 | 2017-06-30 | 北京化工大学 | A kind of high-throughput nucleic acid analysis device based on micro-fluidic chip |
| CN107238716A (en) * | 2017-06-05 | 2017-10-10 | 江苏睿玻生物科技有限公司 | A kind of luminous detection work station of microfluid biochip |
| CN207036852U (en) * | 2017-07-03 | 2018-02-23 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system |
| CN108508193A (en) * | 2018-04-02 | 2018-09-07 | 基蛋生物科技股份有限公司 | A kind of fully-automatic dry type biochemical detection device |
| CN110161003A (en) * | 2019-05-17 | 2019-08-23 | 深圳市刚竹医疗科技有限公司 | Optical detection apparatus and real time fluorescent quantitative nucleic acid amplification detection system |
-
2019
- 2019-10-08 CN CN201910948911.XA patent/CN110726849A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050221281A1 (en) * | 2003-01-08 | 2005-10-06 | Ho Winston Z | Self-contained microfluidic biochip and apparatus |
| CN203908819U (en) * | 2014-06-12 | 2014-10-29 | 云南大学 | Automatic real-time collecting device for runoff water sample |
| CN105572407A (en) * | 2016-01-27 | 2016-05-11 | 广州万孚生物技术股份有限公司 | Full-automatic fluorescent quantitative immunity analyzer and detection method |
| CN106906137A (en) * | 2017-03-19 | 2017-06-30 | 北京化工大学 | A kind of high-throughput nucleic acid analysis device based on micro-fluidic chip |
| CN107238716A (en) * | 2017-06-05 | 2017-10-10 | 江苏睿玻生物科技有限公司 | A kind of luminous detection work station of microfluid biochip |
| CN207036852U (en) * | 2017-07-03 | 2018-02-23 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system |
| CN108508193A (en) * | 2018-04-02 | 2018-09-07 | 基蛋生物科技股份有限公司 | A kind of fully-automatic dry type biochemical detection device |
| CN110161003A (en) * | 2019-05-17 | 2019-08-23 | 深圳市刚竹医疗科技有限公司 | Optical detection apparatus and real time fluorescent quantitative nucleic acid amplification detection system |
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