CN110623001A - Mosquito killing composition containing natural plant essential oil synergist and application thereof - Google Patents
Mosquito killing composition containing natural plant essential oil synergist and application thereof Download PDFInfo
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- CN110623001A CN110623001A CN201910980396.3A CN201910980396A CN110623001A CN 110623001 A CN110623001 A CN 110623001A CN 201910980396 A CN201910980396 A CN 201910980396A CN 110623001 A CN110623001 A CN 110623001A
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- essential oil
- deltamethrin
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- 239000000341 volatile oil Substances 0.000 title claims abstract description 49
- 239000000203 mixture Substances 0.000 title claims abstract description 42
- 241000255925 Diptera Species 0.000 title claims abstract description 28
- 239000005892 Deltamethrin Substances 0.000 claims abstract description 40
- 229960002483 decamethrin Drugs 0.000 claims abstract description 40
- OWZREIFADZCYQD-NSHGMRRFSA-N deltamethrin Chemical compound CC1(C)[C@@H](C=C(Br)Br)[C@H]1C(=O)O[C@H](C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 OWZREIFADZCYQD-NSHGMRRFSA-N 0.000 claims abstract description 33
- 241000196324 Embryophyta Species 0.000 claims abstract description 27
- 244000269722 Thea sinensis Species 0.000 claims abstract description 25
- 235000009569 green tea Nutrition 0.000 claims abstract description 25
- 239000000575 pesticide Substances 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims description 13
- 239000013598 vector Substances 0.000 claims description 7
- 201000004792 malaria Diseases 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 19
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 231100000053 low toxicity Toxicity 0.000 abstract description 4
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 12
- 239000008267 milk Substances 0.000 description 12
- 235000013336 milk Nutrition 0.000 description 12
- 210000004080 milk Anatomy 0.000 description 12
- 238000007796 conventional method Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- 239000000839 emulsion Substances 0.000 description 6
- 238000009472 formulation Methods 0.000 description 6
- 206010059866 Drug resistance Diseases 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 5
- 239000002728 pyrethroid Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 241000256173 Aedes albopictus Species 0.000 description 4
- SECXISVLQFMRJM-UHFFFAOYSA-N N-Methylpyrrolidone Chemical compound CN1CCCC1=O SECXISVLQFMRJM-UHFFFAOYSA-N 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 239000004530 micro-emulsion Substances 0.000 description 4
- 230000001502 supplementing effect Effects 0.000 description 4
- 239000004495 emulsifiable concentrate Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000000361 pesticidal effect Effects 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- 239000004563 wettable powder Substances 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 239000005946 Cypermethrin Substances 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102100031787 Myosin regulatory light polypeptide 9 Human genes 0.000 description 1
- 101710107065 Myosin regulatory light polypeptide 9 Proteins 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- VEMKTZHHVJILDY-UXHICEINSA-N bioresmethrin Chemical compound CC1(C)[C@H](C=C(C)C)[C@H]1C(=O)OCC1=COC(CC=2C=CC=CC=2)=C1 VEMKTZHHVJILDY-UXHICEINSA-N 0.000 description 1
- 239000006229 carbon black Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229960005424 cypermethrin Drugs 0.000 description 1
- KAATUXNTWXVJKI-UHFFFAOYSA-N cypermethrin Chemical compound CC1(C)C(C=C(Cl)Cl)C1C(=O)OC(C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 KAATUXNTWXVJKI-UHFFFAOYSA-N 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229960000490 permethrin Drugs 0.000 description 1
- RLLPVAHGXHCWKJ-UHFFFAOYSA-N permethrin Chemical compound CC1(C)C(C=C(Cl)Cl)C1C(=O)OCC1=CC=CC(OC=2C=CC=CC=2)=C1 RLLPVAHGXHCWKJ-UHFFFAOYSA-N 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000001256 steam distillation Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000304 virulence factor Substances 0.000 description 1
- 230000007923 virulence factor Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a mosquito killing composition containing natural plant essential oil synergist, aiming at providing a mosquito killing composition with obvious synergistic effect, low toxicity and environmental protection, which is prepared from green tea essential oil and deltamethrin according to the weight ratio of 1-300: 1, preparing a composition; belongs to the technical field of pesticides.
Description
Technical Field
The invention discloses a mosquito killing composition, in particular to a mosquito killing composition containing natural plant essential oil synergist, belonging to the technical field of pesticides.
Background
Deltamethrin is a pyrethroid insecticide that plays a key role in controlling malaria vectors and killing and preventing a wide variety of household pests, and is used as a vector for controlling malaria. And also plays an important role in agricultural use and household pests, and controls the use of deltamethrin, either together with or as a substitute for permethrin, cypermethrin, although deltamethrin is a commonly used pyrethroid insecticide. But today the resistance of malaria vectors to deltamethrin (and its counterpart) is now very widespread and threatens the success of the global vector control program.
The synergist is an important auxiliary agent of the current pesticide, has no direct poisoning activity on pests, and can obviously reduce the dosage and increase the pesticide effect when being mixed with the pesticide. Meanwhile, the use of the synergist is also one of important ways for controlling and delaying the current universal generation of drug resistance. The synergistic ether (PBO) has obvious synergistic effect on the pyrethroid medicaments, and is one of the widely accepted synergists with better synergistic activity at present. However, the safety problem of the synergistic ether is always a problem, and the synergistic activity of the synergistic ether is obviously reduced after long-term use.
The long-term use of chemical pesticides causes serious environmental pollution and increases the drug resistance, and the development of botanical pesticides which have broad insecticidal spectrum, low toxicity, difficult generation of drug resistance and environmental friendliness is a current hot topic. The essential oil is volatile aromatic substance extracted from flowers, leaves, stems, roots or fruits of plants by steam distillation, extrusion, cold soaking or solvent extraction, and when different chemical components are used in combination with different plant essential oils, the effective components contained in the essential oil may show synergistic effect, and the use concentration of chemical pesticides can be reduced to a certain extent. Plant essential oils are now becoming a powerful alternative to PBO for the selection of synergistic pyrethroid drugs. Many plant essential oils exhibit significant synergistic activity against deltamethrin-based drugs.
Disclosure of Invention
In order to solve the problems of gradually increased resistance of the malaria mosquito vector agricultural crops to chemical pesticides, environmental pollution and the like in the prior art, the invention aims to provide the mosquito killing composition which has obvious synergistic action, low toxicity and environmental friendliness.
Therefore, the first technical scheme provided by the invention is as follows:
a mosquito killing composition containing natural plant essential oil synergist is prepared from green tea essential oil and deltamethrin according to the weight ratio of 1-300: 1 is prepared from (1).
As a further preferred aspect of the present invention, the above-mentioned mosquito-killing composition containing natural plant essential oil synergist is prepared from green tea essential oil and deltamethrin according to the ratio of 75-300: 1 is prepared from (1).
As a further preferred aspect of the present invention, the above-mentioned mosquito-killing composition containing natural plant essential oil synergist is prepared from green tea essential oil and deltamethrin according to the ratio of 150: 1 is prepared from (1).
The second technical scheme provided by the invention is the application of the mosquito killing composition containing the natural plant essential oil synergist as a mosquito vector for preventing and treating the malaria of crops.
The third technical scheme is that the pesticide preparation is prepared from a mosquito killing composition containing a natural plant essential oil synergist and an agriculturally and pharmaceutically acceptable auxiliary material.
Wherein, the mosquito killing composition containing the natural plant essential oil synergist accounts for 2 to 20 percent of the total mass of the pesticide preparation.
Compared with the prior art, the technical scheme provided by the invention has the following technical advantages:
(1) according to the technical scheme provided by the invention, the green tea essential oil and the deltamethrin are combined according to a specific proportion, so that the synergistic effect is remarkable, the dosage is reduced, and pests with resistance to the existing pyrethroid medicaments can be effectively prevented.
(2) The technical scheme provided by the invention has the advantages that the action sites are increased, the drug resistance of pests is favorably overcome and delayed, and the use life of the pesticide is prolonged.
(3) The synergistic component provided by the technical scheme of the invention is a plant component, and has the advantages of low toxicity, difficult generation of drug resistance, environmental friendliness and the like.
Detailed Description
The technical solutions in the embodiments of the present invention are described below clearly and completely, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. For process parameters or conditions not specifically mentioned, it can be carried out with reference to conventional techniques.
Example 1
The invention provides a mosquito killing composition containing natural plant essential oil synergist, which is prepared from the following components in parts by mass: 1, and deltamethrin.
Example 2
The invention provides another mosquito killing composition containing natural plant essential oil synergist, which is prepared from the following components in percentage by mass of 150: 1, and deltamethrin.
Example 3
The invention provides another mosquito killing composition containing natural plant essential oil synergist, which is prepared from the following components in percentage by mass of 100: 1, and deltamethrin.
Example 4
The invention provides another mosquito killing composition containing natural plant essential oil synergist, which is prepared from the following components in percentage by mass of 75: 1, and deltamethrin.
Example 5
The invention provides another mosquito killing composition containing natural plant essential oil synergist, which is prepared from the following components in percentage by mass of 50: 1, and deltamethrin.
Example 6
The invention provides another mosquito killing composition containing natural plant essential oil synergist, which is prepared from the following components in percentage by mass: 1, and deltamethrin.
Example 7
The invention provides another mosquito killing composition containing natural plant essential oil synergist, which is prepared from the following components in percentage by mass: 1, and deltamethrin.
For convenience of use, the pesticidal composition of the present invention may be prepared into a formulation suitable for agricultural use by a known method.
Example 8 aqueous emulsion formulation 1
The preparation is prepared from the following components in percentage by mass: 15% of green tea essential oil, 0.1% of deltamethrin, 5% of N-methyl pyrrolidone, 1601# 2% of agricultural milk, 2201# 5% of agricultural milk, 700# 2% of agricultural milk, 5% of propylene glycol, 0.05% of glacial acetic acid and deionized water for supplementing 100%. The components are processed by a conventional method to prepare the 15.1 percent green tea essential oil-deltamethrin emulsion in water.
Example 9 aqueous emulsion formulation 2
The preparation is prepared from the following components in percentage by mass: 7.5% of green tea essential oil, 0.1% of deltamethrin, 5% of N-methyl pyrrolidone, 1601# 2% of agricultural milk, 2201# 3% of agricultural milk, 700# 2% of agricultural milk, 5% of propylene glycol, 0.05% of glacial acetic acid and deionized water for making up 100%. The components are processed by a conventional method to prepare the 7.6 percent green tea essential oil-deltamethrin emulsion in water.
EXAMPLE 8 microemulsion preparation 1
The preparation is prepared from the following components in percentage by mass: 2% of green tea essential oil, 2% of deltamethrin, 10% of N-methyl pyrrolidone, 600# 8% of agricultural milk, 2201# 3% of agricultural milk, 5% of propylene glycol and deionized water for making up 100%. The components are processed by a conventional method to prepare the green tea essential oil-deltamethrin microemulsion with the concentration of 4 percent.
Example 9 microemulsion formulation 2
The preparation is prepared from the following components in percentage by mass: 0.2% of green tea essential oil, 2% of deltamethrin, 10% of N-methyl pyrrolidone, 600# 8% of agricultural milk, 2201# 3% of agricultural milk, 5% of propylene glycol and deionized water for supplementing 100%. Processing the above components by conventional method to obtain 2.2% green tea essential oil-deltamethrin microemulsion.
EXAMPLE 10 emulsifiable concentrate formulation 1
The preparation is prepared from the following components in percentage by mass: 5% of green tea essential oil, 0.1% of deltamethrin, 2201# 10% of agricultural emulsion, 2201# 3% of agricultural emulsion and xylene for supplementing 100%. The components are processed by a conventional method to prepare the green tea essential oil-deltamethrin emulsifiable concentrate with the concentration of 5.1 percent.
EXAMPLE 11 emulsifiable concentrate formulation 2
The preparation is prepared from the following components in percentage by mass: 10% of green tea essential oil, 1% of deltamethrin, 2201# 10% of agricultural milk, 2201# 3% of agricultural milk and xylene for supplementing 100%. The components are processed by a conventional method to prepare 11 percent green tea essential oil-deltamethrin emulsifiable solution.
EXAMPLE 12 wettable powder
The preparation is prepared from the following components in percentage by mass: 10% of green tea essential oil, 10% of deltamethrin, 2% of sodium dodecyl benzene sulfonate, 5% of NNO, 6% of sodium lignin, 8% of white carbon black 4%, and the balance of diatomite to 100%. The components are processed by a conventional method to prepare 20% of green tea essential oil-deltamethrin wettable powder.
The above essential oils are purchased from Yuejing essential oils Co.
To better illustrate the technical advantages of the technical solution provided by the present invention, the following experimental data of the compositions provided by the present application are given:
experiment one: determination of toxicity activity of green tea essential oil and deltamethrin compounded on aedes albopictus
Test insects: aedes albopictus
The test method is a Tarsal test method, and the synergistic activity is tested by independently matching the concentration of deltamethrin poisoning activity LC20 with various essential oils and PBO. Selecting various essential oil with the concentration of 35 mu g/m2And in the deltamethrin test, uniformly spraying a sample to be tested on a culture dish, shaking up, covering by using a self-made cover after the solvent is completely volatilized, and transferring 20 mosquitoes to be tested into the culture dish by using a mosquito sucker. The number of mosquito knockdown deaths was recorded after 1 hour of exposure and subsequently transferred to disposable paper cups and placed in sugar water. The mortality was observed after 24 hours of incubation at 26 ℃. + -. 2 ℃ and 70%. + -. 5% relative humidity. The toxicity regression equation of the samples was then calculated using the software SPSS24.0 and origin2017, and the toxicity factor of the combination mixture of plant essential oils and pyrethroids was calculated using the Mansour equation. A mixture with virulence factors greater than 20 is defined as having a synergistic effect and a value below 20 is defined as having an antagonistic effect.
The co-toxicity coefficient (actual mortality-theoretical mortality)/theoretical mortality, and the toxicity test results are shown in table 1.
TABLE 1 toxicity test results of synergistic composition of green tea essential oil and deltamethrin against Aedes albopictus
As can be seen from table 1, when the green tea essential oil and the deltamethrin are in a specific proportioning range, the pesticide composition has a remarkable synergistic effect on aedes albopictus; particularly, when the mass ratio of the plant essential oil to the deltamethrin is 1: 150 hours, the compound shows strong synergistic effect, and is higher than the synergistic activity of PBO under the same test condition.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, simplifications and equivalents which do not depart from the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. A mosquito killing composition containing natural plant essential oil synergist is characterized in that the composition is prepared from green tea essential oil and deltamethrin according to the weight ratio of 1-300: 1 is prepared from (1).
2. The composition of claim 1, wherein the composition comprises green tea essential oil and deltamethrin in a ratio of 75-300: 1 is prepared from (1).
3. The composition of claim 2, wherein the composition comprises green tea essential oil and deltamethrin in a ratio of 150: 1 is prepared from (1).
4. The use of a natural plant essential oil potentiator-containing mosquito killing composition of claim 1 as a mosquito vector medicament for the control of malaria in crops.
5. A pesticide preparation, which is characterized by comprising the mosquito killing composition containing the natural plant essential oil synergist in claim 1 and an agriculturally and pharmaceutically acceptable auxiliary material.
6. The pesticide preparation of claim 1, wherein the mosquito killing composition containing the natural plant essential oil synergist accounts for 2-20% of the total mass of the pesticide preparation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910980396.3A CN110623001B (en) | 2019-10-15 | 2019-10-15 | Mosquito killing composition containing natural plant essential oil synergist and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910980396.3A CN110623001B (en) | 2019-10-15 | 2019-10-15 | Mosquito killing composition containing natural plant essential oil synergist and application thereof |
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| CN110623001A true CN110623001A (en) | 2019-12-31 |
| CN110623001B CN110623001B (en) | 2021-04-30 |
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