CN110619927A - 一种实时荧光定量pcr的数据分析方法 - Google Patents
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- 238000007405 data analysis Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000003753 real-time PCR Methods 0.000 title claims abstract description 22
- 230000003321 amplification Effects 0.000 claims abstract description 24
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 24
- 238000004458 analytical method Methods 0.000 claims abstract description 5
- 238000012417 linear regression Methods 0.000 claims abstract description 4
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000004364 calculation method Methods 0.000 abstract description 4
- 108020004707 nucleic acids Proteins 0.000 abstract description 3
- 102000039446 nucleic acids Human genes 0.000 abstract description 3
- 150000007523 nucleic acids Chemical class 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 12
- 239000000523 sample Substances 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 10
- 108020004414 DNA Proteins 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 4
- 230000014509 gene expression Effects 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 229910014455 Ca-Cb Inorganic materials 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108700019961 Neoplasm Genes Proteins 0.000 description 1
- 102000048850 Neoplasm Genes Human genes 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 238000012339 Real-time fluorescence quantitative polymerase chain reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
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- 238000003205 genotyping method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 238000012544 monitoring process Methods 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
样本量 | 10<sup>7</sup> | 10<sup>6</sup> | 10<sup>5</sup> | 10<sup>4</sup> |
Ct | 18.77 | 22.32 | 26.28 | 29.69 |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111593098A (zh) * | 2020-05-29 | 2020-08-28 | 成都瀚辰光翼科技有限责任公司 | 一种qpcr实时荧光数据定量分析的方法 |
CN112342282A (zh) * | 2020-08-25 | 2021-02-09 | 杭州博日科技股份有限公司 | 荧光定量的指标确定方法 |
CN112582026A (zh) * | 2020-12-07 | 2021-03-30 | 上海科源电子科技有限公司 | 基于效率的qPCR初始浓度检测的高鲁棒性六参数全局拟合法 |
CN112652363A (zh) * | 2020-12-30 | 2021-04-13 | 杭州博日科技股份有限公司 | 熔解曲线异常值处理方法、装置以及电子设备 |
CN112862297A (zh) * | 2021-02-02 | 2021-05-28 | 方舟生物安全科技(广州)有限公司 | 一种病原微生物物联网实时监测*** |
WO2022141674A1 (zh) * | 2020-12-30 | 2022-07-07 | 北京中科生仪科技有限公司 | 一种pcr检测仪及其方法 |
CN115985396A (zh) * | 2022-12-16 | 2023-04-18 | 苏州思迈德生物科技有限公司 | 一种实时荧光定量pcr扩增数据的分析处理方法及装置 |
CN117411918A (zh) * | 2023-12-11 | 2024-01-16 | 深圳前海翼联科技有限公司 | 一种基于iot物联网的监控报警方法及*** |
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CN101144771A (zh) * | 2006-09-11 | 2008-03-19 | 中山大学达安基因股份有限公司 | 检测人类免疫缺陷病毒的方法及试剂盒 |
CN101240351A (zh) * | 2008-03-20 | 2008-08-13 | 山东出入境检验检疫局检验检疫技术中心 | 淋巴囊肿病毒的实时定量pcr检测方法 |
CN101533010A (zh) * | 2009-02-27 | 2009-09-16 | 中国人民解放军第四军医大学 | 一种血清中MG7-Ag的定量检测方法 |
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CN107016258A (zh) * | 2016-01-27 | 2017-08-04 | 应清界 | 一种基于重组酶介导等温核酸扩增(raa)法进行荧光定量计算的方法 |
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CN109212222A (zh) * | 2017-07-05 | 2019-01-15 | 颜云华 | 糖尿病孕妇维生素d代谢与糖代谢相关性及胎盘检测方法 |
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Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111593098B (zh) * | 2020-05-29 | 2021-09-21 | 成都瀚辰光翼科技有限责任公司 | 一种qpcr实时荧光数据定量分析的方法 |
CN111593098A (zh) * | 2020-05-29 | 2020-08-28 | 成都瀚辰光翼科技有限责任公司 | 一种qpcr实时荧光数据定量分析的方法 |
CN112342282A (zh) * | 2020-08-25 | 2021-02-09 | 杭州博日科技股份有限公司 | 荧光定量的指标确定方法 |
CN112582026A (zh) * | 2020-12-07 | 2021-03-30 | 上海科源电子科技有限公司 | 基于效率的qPCR初始浓度检测的高鲁棒性六参数全局拟合法 |
CN112582026B (zh) * | 2020-12-07 | 2022-07-12 | 上海科源电子科技有限公司 | 基于效率的qPCR初始浓度检测的高鲁棒性六参数全局拟合法 |
WO2022141674A1 (zh) * | 2020-12-30 | 2022-07-07 | 北京中科生仪科技有限公司 | 一种pcr检测仪及其方法 |
CN112652363A (zh) * | 2020-12-30 | 2021-04-13 | 杭州博日科技股份有限公司 | 熔解曲线异常值处理方法、装置以及电子设备 |
CN112862297B (zh) * | 2021-02-02 | 2022-03-01 | 方舟生物安全科技(广州)有限公司 | 一种病原微生物物联网实时监测*** |
CN112862297A (zh) * | 2021-02-02 | 2021-05-28 | 方舟生物安全科技(广州)有限公司 | 一种病原微生物物联网实时监测*** |
CN115985396A (zh) * | 2022-12-16 | 2023-04-18 | 苏州思迈德生物科技有限公司 | 一种实时荧光定量pcr扩增数据的分析处理方法及装置 |
CN115985396B (zh) * | 2022-12-16 | 2023-12-12 | 苏州思迈德生物科技有限公司 | 一种实时荧光定量pcr扩增数据的分析处理方法及装置 |
CN117411918A (zh) * | 2023-12-11 | 2024-01-16 | 深圳前海翼联科技有限公司 | 一种基于iot物联网的监控报警方法及*** |
CN117411918B (zh) * | 2023-12-11 | 2024-04-02 | 深圳前海翼联科技有限公司 | 一种基于iot物联网的监控报警方法及*** |
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Application publication date: 20191227 Assignee: Beijing Linke Biotechnology Co.,Ltd. Assignor: BEIJING INTEGRATED BIOSYSTEMS Co.,Ltd. Contract record no.: X2022990000932 Denomination of invention: A Data Analysis Method of Real time Fluorescence Quantitative PCR Granted publication date: 20220628 License type: Common License Record date: 20221114 |
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Application publication date: 20191227 Assignee: Beijing Linke Biotechnology Co.,Ltd. Assignor: BEIJING INTEGRATED BIOSYSTEMS Co.,Ltd. Contract record no.: X2022990000932 Denomination of invention: A data analysis method for real-time fluorescence quantitative PCR Granted publication date: 20220628 License type: Common License Record date: 20221114 |