CN110585302A

CN110585302A - Traditional Chinese medicine combined granular tea for treating chronic pharyngitis and preparation method thereof

Info

Publication number: CN110585302A
Application number: CN201910983817.8A
Authority: CN
Inventors: 张宇; 邱洪斌; 赵宝江; 周淑晶; 刘翠娟; 李爽; 孟繁玲; 张曼; 刘佳蕾
Original assignee: Jiamusi University
Current assignee: Jiamusi University
Priority date: 2019-10-16
Filing date: 2019-10-16
Publication date: 2019-12-20

Abstract

The invention discloses a traditional Chinese medicine combined granular tea for treating chronic pharyngitis and a preparation method thereof. Relates to the technical field of traditional Chinese medicines and functional foods. The traditional Chinese medicine composite granular tea comprises the following components in parts by weight: 4-6 parts of honeysuckle extract, 4-6 parts of chrysanthemum extract, 4-6 parts of dandelion extract, 4-6 parts of boat-fruited sterculia seed extract, 8-12 parts of medlar extract and 65-80 parts of auxiliary materials. The preparation method of the traditional Chinese medicine composite granular tea comprises the following steps: preparing each extract, preparing soft materials, extruding and molding, and drying to obtain the finished product. The invention has the following beneficial effects: the selected medicinal materials have wide sources, the medicines are applied to organic compatibility, the synergistic effect is realized, the treatment of both symptoms and root causes is realized, the price is low, the medicine property is mild, the medicine is safe and reliable, and the effects of clearing and moistening throat and reducing sore throat are realized.

Description

Traditional Chinese medicine combined granular tea for treating chronic pharyngitis and preparation method thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines and functional foods, in particular to a traditional Chinese medicine combined granular tea for treating chronic pharyngitis and a preparation method thereof.

Background

In recent years, due to the rapid rise of the industrial development of China and the improvement of the quality of life of people, the air quality is increasingly not fresh, the dust visible to the naked eyes is increased, harmful gas is irritated, various food choices are made, and the consumption of tobacco and wine is excessive. At present, people who excessively use voices tend to rise year by year, such as teachers, salespeople, singers and the like. Thus, the condition pharyngitis comes into the field of vision and is a common, highly developed condition, clinically referred to as incurable disease. In the past decades, no attention is paid to medical prevention and treatment, no corresponding treatment method or medicine is provided, only the anti-inflammatory medicine is used for treatment, the obtained curative effect is not obvious, and the symptoms of chronic pharyngitis and the mood and spirit of an individual have certain influence. At present, the incidence rate of the pharyngolaryngitis is on a rising trend, so people pay more attention to the selection of the medicines for clearing heat and relieving sore throat so as to relieve and treat discomfort caused by the chronic pharyngitis.

Modern medicine considers chronic pharyngitis as a common throat disease, usually due to infections of the throat, with inflammation occurring mainly at the mucous membrane of the throat of humans. Because the throat is positioned at the crossing position of the respiratory tract and the digestive tract, the throat is subjected to stimulation of physical or chemical factors and the like caused by external food, dust and pollen in the air and occupational work reasons for a long time, and the conventional stimulation can cause discomfort of the throat and further cause chronic pharyngitis. In addition, various chronic diseases can also induce chronic pharyngitis, which is mostly caused by adults, has long course of disease and stubborn symptoms and is not easy to cure. Clinically, pathogenic bacteria with high pathogenic capability which are easy to cause chronic pharyngitis are pathogenic bacteria such as beta hemolytic streptococcus, staphylococcus aureus and the like, and the pathogenic bacteria provide basis for researching preclinical effectiveness evaluation of related medicines. Research shows that various inflammatory factors are closely related to the occurrence of chronic pharyngitis, wherein Prostaglandin (PGE) exists in inflammatory tissues and can act synergistically with inflammatory mediators such as bradykinin to reduce pain threshold, activate a peripheral sensory nerve terminal (EP) receptor, sensitize a peripheral pain receptor and cause hyperalgesia to play an inflammation-causing effect; interleukin-1 β (1L-1 β), an important cytokine and polypeptide regulator, is produced primarily by mononuclear macrophages. It plays an important regulatory role in cell immune activation, has wide biological activity and can cause an important factor of chronic inflammation; tumor necrosis factor (TNF- α) is mainly used to mediate anti-tumor and regulate immune function of the body, and is also involved in various pathological changes of inflammatory diseases. The research of detecting inflammatory factors in human serum is convenient to determine whether the medicine promotes the repair of pharyngeal mucosa inflammation.

The traditional Chinese medicine considers that chronic pharyngitis is caused by incomplete treatment of wind-heat pharyngitis, or failure of residual evil after warm and hot pain or failure of pharynx nourishment due to overeating spicy food. The treatment principle is that 'benefiting yin, clearing heat, regulating qi, nourishing yin, reducing fire and removing heat' is mostly adopted. In the past, various medicines for treating pharyngitis exist in the market, but most of the medicines are western medicines, mainly take antibiotic anti-inflammation as a main part, and long-term use of antibiotics can cause the organism to generate drug resistance, so that the drug resistance is easy to relapse, and the disease cannot be eradicated fundamentally. In addition, most western medicines have certain side effects and can cause harm to human health. In recent years, the throat-clearing products of traditional Chinese medicines and traditional Chinese medicine compound preparations are mostly throat-clearing buccal tablets, preserved fruits and the like, are mainly prepared from fruit raw materials with the effects of clearing heat and relieving throat, such as Chinese olive, Chinese wampee, plum and the like, and have poor curative effect on pharyngitis. So far, few researches are made on the traditional Chinese medicine granular tea for clearing and moistening the throat.

Therefore, the problem to be solved by the technical personnel in the field is urgently needed to provide the medicine for treating the chronic pharyngitis, which has good treatment effect, scientific dosage form and small toxic and side effects.

Disclosure of Invention

In view of the above, the invention provides a traditional Chinese medicine composite granular tea for treating chronic pharyngitis and a preparation method thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

a traditional Chinese medicine composite granular tea for treating chronic pharyngitis comprises the following components in parts by mass:

honeysuckle flower, flos Lonicerae, sweet in nature and taste, cold in nature, enters lung, heart and stomach meridians. Its main functions are dispelling wind and heat, clearing away heat and toxic material, etc. It is commonly used for treating wind-heat type common cold, sore throat, and respiratory infection; diarrhea, dysentery, sores and ulcers. The honeysuckle flower contains various effective components such as chlorogenic acid, ginkgol, volatile oil, flavonoid and the like. Particularly, flavonoid components such as quercetin and glycosides thereof, luteolin and glycosides thereof and the like contained in the honeysuckle flower have strong inhibitory effect on various pathogenic bacteria such as hemolytic streptococcus, staphylococcus aureus and the like and upper respiratory infection pathogenic viruses and the like. Has antipyretic, hepatoprotective, antiinflammatory, antibacterial, anti-adenovirus, and antiviral effects.

The chrysanthemum is sweet, bitter and slightly cold in nature and belongs to liver and lung channels, has the main effects of clearing heat and detoxicating, calming the liver and improving eyesight, and dispelling wind and clearing heat, and is mainly used for treating wind-heat type common cold, dizziness, conjunctival congestion and swelling pain, blurred vision, sore and carbuncle pyogenic infections. The chrysanthemum contains various effective components such as flavonoid, terpenoid, organic acid and the like. Has antibacterial, antiinflammatory, blood lipid reducing, antitumor, antioxidant, blood vessel relaxing, and plumbum expelling effects.

Dandelion is sweet in nature and taste, slightly bitter and cold, entering stomach and liver meridians. Mainly has the efficacies of diuresis, cholagogue, laxation, jaundice reduction and the like. The herba Taraxaci contains various active ingredients such as taraxol, taraxacin, choline, organic acid, and inulin. It is used to treat toxic heat, carbuncle, conjunctival congestion, swelling and pain, jaundice, stranguria with urine, toothache, pharyngalgia, acute mastitis, lymphadenitis, acute conjunctivitis, common cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, and urinary tract infection. The dandelion is a substance used as both medicine and food, and research shows that the dandelion tablet prepared from the dandelion has the effect of moistening the throat.

Boat-fruited sterculia seed is cold in nature and sweet in taste. It has the functions of relieving sore throat, clearing away lung heat, eliminating phlegm, moistening intestine and relaxing bowels. Mainly contains galacturonic acid, arabinose, galactacetic acid, galactose, fat and large sea element, tragacanth, etc. It is mainly used for treating dry cough without phlegm, laryngalgia, hoarseness, internal heat due to bone steaming, hematemesis, epistaxis, hematochezia, conjunctival congestion, etc. Has the pharmacological effects of relieving diarrhea, lowering blood pressure, etc.

The honeysuckle, the chrysanthemum, the boat-fruited sterculia seed and the dandelion are used together, the honeysuckle is used for clearing heat toxin of heart and stomach, the chrysanthemum is used for tonifying yin, clearing heat and removing toxicity, the boat-fruited sterculia seed has the curative effect of relieving the throat inflammation, and the dandelion has the functions of moistening throat and nourishing. The honeysuckle and the boat-fruited sterculia seed can inhibit or kill various bacteria when being used together; after the chrysanthemum and the dandelion are compatible, the immunity of the human body can be obviously improved, and the invasion of viruses is effectively reduced. The honeysuckle flower, the chrysanthemum, the dandelion and the boat-fruited sterculia seed in the formula are compatible, so that the heat-clearing and fire-purging traditional Chinese medicine can be used for clearing heat and purging fire and treating sore throat.

Wolfberry fruit, fructus Lycii, with sweet and neutral nature and flavor, enters liver and kidney meridians, and has effects of nourishing liver and kidney, replenishing vital essence and improving eyesight. Has multiple health promotion effects, is a substance used as both medicine and food, contains multiple components such as polysaccharides, amino acids, microelements, alkaloids and inorganic salts, wherein fructus Lycii polysaccharide is the main active component for exerting its pharmacological action. The medlar has the functions of improving the immunologic function, protecting the liver, improving the eyesight, delaying the senility, resisting the tumor, improving the memory and the like, and has very good health-care effect due to the active ingredients such as zeaxanthin, baccatin and the like contained in the medlar.

The medlar contains rich medlar polysaccharide, beta-carotene, vitamin E, selenium, flavonoid and the like, and the components have better antioxidation. Meanwhile, the medlar is suitable for eating all the year round, and can improve the body constitution and enhance the immunity after being drunk frequently. The honeysuckle, the chrysanthemum, the dandelion and the boat-fruited sterculia seed are all compatible with cold medicines, so that the stomach can be injured after long-term taking, and after the warm-nourishing wolfberry fruit is added, the medicine property is neutralized, the curative effects of clearing away heat and relieving sore throat, reducing swelling and relieving pain and the like can be enhanced, and the throat-moistening health-care tea is suitable for people who take excessive throat for a long time.

Because the components supplement each other and cooperate with each other to generate a synergistic effect, the compatibility of the medicines is comprehensive and comprehensive, the primary and secondary are clear, the advantages of the medicines are improved, the defects of the medicines are overcome, the yang is introduced into the yin, the yin is harmonized with the yin, the diseases are self-healed, the treatment effect on the chronic pharyngitis is obvious, the medicines are less effective, the symptoms and root causes are treated, and the effect of twice as much as possible is achieved.

The addition of the auxiliary materials plays a great role in the comfortable taking and can enhance the stability of the granular tea preparation.

The invention processes the product into the traditional Chinese medicine granular tea which is simple to drink, unique in taste and convenient to carry. The pharmaceutical composition has the advantages of remarkable curative effect, small toxic and side effects, scientific formulation, convenient use and low cost. Is suitable for most people and has a large market.

Preferably, the auxiliary materials comprise the following components in parts by weight:

according to the technical scheme, the auxiliary materials are in parts by weight, so that the heat-clearing and inflammation-diminishing effect is optimal, the forming rate of the granular tea can reach more than 85%, and the related indexes such as moisture absorption rate, moisture content, solubility and the like reach the production quality standard of granules. In addition, the adding amount of the natural menthol, the sodium carboxymethyl cellulose, the potassium sorbate and the sodium citrate is performed according to the beverage regulation of the national standard of food safety.

A preparation method of traditional Chinese medicine combined granular tea for treating chronic pharyngitis comprises the following steps:

(1) respectively preparing honeysuckle extract, chrysanthemum extract, dandelion extract, boat-fruited sterculia seed extract and medlar extract by a hot water extraction method;

(2) weighing and uniformly mixing the honeysuckle extract, the chrysanthemum extract, the dandelion extract, the sterculia scaphigera extract, the medlar extract and auxiliary materials according to the mass ratio to obtain a mixed soft material for later use;

(3) extruding the mixed soft material, and sieving with a 16-mesh sieve to obtain a granular substance;

(4) and drying the granular substances, and screening the granular substances by a medicine inspection screen to obtain a finished product.

Preferably, the step (1) is specifically operated as follows:

(11) soaking: respectively mixing honeysuckle, chrysanthemum, dandelion, sterculia lychnophora, wolfberry fruit and drinking water according to the mass material-liquid ratio of 1 (15-25), and soaking for 1-3 hours;

(12) hot water leaching: after soaking, heating and extracting, wherein the extraction temperature is 80-100 ℃, the extraction time is 1.5-2 h, extracting for 2 times, filtering, and combining filtrates to respectively obtain a honeysuckle extract, a chrysanthemum extract, a dandelion extract, a sterculia lychnophora extract and a wolfberry fruit extract;

(13) respectively centrifuging the honeysuckle extract, the chrysanthemum extract, the dandelion extract, the sterculia lychnophora extract and the wolfberry fruit extract at 4000r/min at 20-25 ℃ for 10min, and concentrating under reduced pressure (steam pressure is 0.2MPa, temperature is 45-60 ℃) until the relative density (the reference ratio is the density of water) is 1.2-1.4 to respectively obtain honeysuckle extract, chrysanthemum extract, dandelion extract, sterculia lychnophora extract and wolfberry fruit extract.

Preferably, in the step (11), the ratio of the honeysuckle, the dandelion, the medlar and the drinking water is 1 (15-20), and the ratio of the chrysanthemum, the sterculia lychnophora and the drinking water is 1 (20-25).

Preferably, in the step (11), the preferred soaking time of the honeysuckle, the chrysanthemum, the dandelion and the sterculia scaphigera is 1-2 hours, and the preferred soaking time of the medlar is 2-3 hours.

Preferably, in the step (12), the preferred extraction temperature for preparing the honeysuckle extract, the chrysanthemum extract, the dandelion extract and the boat-fruited sterculia seed extract is 90-100 ℃, and the preferred extraction temperature for preparing the medlar extract is 80-90 ℃.

Preferably, in the step (13), the optimal centrifugation temperature of the honeysuckle extract, the chrysanthemum extract, the dandelion extract and the boat-fruited sterculia seed extract is 25 ℃, and the optimal centrifugation temperature of the medlar extract is 20 ℃.

Preferably, the particle substances in the step (4) are dried in an oven at the temperature of 60-70 ℃ for 60-70 min.

Preferably, the step (4) of screening the medicine is to collect dried granular substances which can pass through a No. 1 sieve but cannot pass through a No. 5 sieve, and then a finished product is obtained.

Further, the best process for preparing the honeysuckle extract comprises the following steps:

mixing honeysuckle and drinking water according to a mass material-liquid ratio of 1: 15-20, soaking for 1-2 h, then heating and leaching at 90-100 ℃, extracting twice for 1.5-2 h each time, filtering while hot, combining the two extracting solutions, centrifuging for 10min at 25 ℃ at 4000r/min, and concentrating under reduced pressure (steam pressure 0.2MPa, temperature 55-60 ℃) to obtain a honeysuckle extract with a relative density (reference ratio is the density of water) of 1.2-1.4 (60 ℃).

Further, the best process for preparing the chrysanthemum extract comprises the following steps:

mixing chrysanthemum and drinking water according to a mass material-liquid ratio of 1: 20-25, soaking for 1-2 h, then heating and leaching at 90-100 ℃, extracting for 1.5-2 h, filtering while hot, combining two extracting solutions, centrifuging for 10min at 4000r/min at 25 ℃, and concentrating under reduced pressure (steam pressure is 0.2MPa, temperature is 55-60 ℃) to obtain chrysanthemum extract with a relative density (reference ratio is density of water) of 1.2-1.4 (60 ℃).

Further, the best process for preparing the dandelion extract comprises the following steps:

mixing dandelion and drinking water according to a mass material-liquid ratio of 1: 15-20, soaking for 1-2 h, then heating and leaching at 90-100 ℃, extracting for 1.5-2 h, filtering while hot, combining two extracting solutions, centrifuging for 10min at 4000r/min at 25 ℃, and concentrating under reduced pressure (steam pressure is 0.2MPa, temperature is 50-55 ℃) to obtain dandelion extract with a relative density (reference ratio is water density) of 1.2-1.4 (60 ℃).

Further, the best process for preparing the extract of the scaphium scaphigerum comprises the following steps:

mixing the boat-fruited sterculia seed with drinking water according to the mass material-liquid ratio of 1: 20-25, soaking for 1-2 h, then heating and leaching at 90-100 ℃, extracting for 1.5-2 h, filtering while hot, combining the two extracting solutions, centrifuging for 10min at 4000r/min at 25 ℃, and concentrating under reduced pressure (the steam pressure is 0.2MPa, the temperature is 45-50 ℃) to obtain the boat-fruited sterculia seed extract with the relative density (the reference ratio is the density of water) of 1.2-1.4 (60 ℃).

Further, the best process for preparing the extract of the medlar comprises the following steps:

mixing the wolfberry fruit and drinking water according to the mass ratio of materials to liquid of 1: 15-20, soaking for 2-3 h, then heating and leaching at 80-90 ℃, extracting for 1.5-2 h, filtering while hot, combining two extracting solutions, centrifuging for 10min at 4000r/min at 20 ℃, and concentrating under reduced pressure (steam pressure is 0.2MPa, temperature is 45-50 ℃) to obtain the wolfberry fruit extract with the relative density (reference ratio is the density of water) of 1.2-1.4 (60 ℃).

The invention extracts honeysuckle, chrysanthemum, dandelion, sterculia seed and medlar respectively, adds the extracts of all the components into auxiliary materials, mixes the extracts and prepares the granular tea. According to the characteristics of the components, the extraction and concentration temperatures (the concentration temperature of honeysuckle and chrysanthemum extracting solution is 55-60 ℃, the concentration temperature of dandelion extracting solution is 50-55 ℃, and the concentration temperature of boat-fruited sterculia seed and medlar extracting solution is 45-50 ℃) are respectively controlled, so that the active ingredients or the active parts are prevented from being heated unstably for a long time to cause chemical structure damage. Meanwhile, in the extraction process of the active ingredients of each substance, the material-liquid ratio is determined according to the viscosity of the extracting solution when each component is extracted, and when root medicinal materials and medicinal materials containing more polysaccharide are extracted by water, if the extracting solution is less, the extraction is incomplete, and in addition, the filtration and the concentration are difficult; if the amount of the extract is too large, the extraction rate is not greatly improved, and energy is wasted during concentration.

Through the technical scheme, compared with the prior art, the invention has the following advantages:

(1) directly acts on throat, is beneficial to the direct absorption of mucosa, immediately enters the submucosa, directly participates in blood circulation, quickly plays the effects of clearing heat, cooling blood, purging fire for removing toxin, nourishing yin and moistening throat and achieves the purposes of clearing away the lung-heat and stomach-dryness fire and relieving sore throat;

(2) the preparation method is simple, and has lower requirements on process equipment;

(3) compared with similar products, the product has the characteristics of convenient carrying, easy drinking and the like, and can be drunk in any occasions;

(4) the health-care tea has the advantages of small dosage, good curative effect, small toxic and side effects, convenience in use, low price and easiness in drinking, has the health-care effect in daily drinking, and is suitable for various crowds.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

(1) Mixing honeysuckle and drinking water according to a mass material-liquid ratio of 1:20, soaking for 2h, heating and leaching at 90 ℃, extracting twice, extracting for 2h for the first time and extracting for 1.5h for the second time, filtering while hot, combining extracting solutions of the two times, centrifuging (25 ℃, 4000r/min and 10min), and concentrating under reduced pressure (steam pressure of 0.2MPa and temperature of 58 ℃) to obtain a honeysuckle extract with a relative density (the reference ratio is the density of water) of 1.3(60 ℃).

(2) Mixing chrysanthemum and drinking water according to a mass material-liquid ratio of 1:25, soaking for 2h, heating and leaching at 90 ℃, extracting twice, extracting for 2h for the first time and extracting for 1.5h for the second time, filtering while hot, combining extracting solutions of the two times, centrifuging (25 ℃, 4000r/min and 10min), and concentrating under reduced pressure (steam pressure of 0.2MPa and temperature of 58 ℃) to obtain chrysanthemum extract with relative density (the reference ratio is the density of water) of 1.3(60 ℃).

(3) Mixing herba Taraxaci with drinking water at a mass ratio of 1:15, soaking for 2h, heating and leaching at 90 deg.C, extracting twice, extracting for 2h for the first time and 1.5h for the second time, filtering while hot, mixing the two extractive solutions, centrifuging (25 deg.C, 4000r/min, 10min), concentrating under reduced pressure (steam pressure 0.2MPa, temperature 53 deg.C) to obtain herba Taraxaci extract with relative density (water density) of 1.3(60 deg.C).

(4) Mixing semen Scaphii Lychnophori and drinking water at a mass ratio of 1:20, soaking for 2 hr, heating and leaching at 90 deg.C, extracting twice, the first extraction for 2 hr and the second extraction for 1.5 hr, filtering while hot, mixing the two extractive solutions, centrifuging (25 deg.C, 4000r/min, 10min), concentrating under reduced pressure (steam pressure of 0.2MPa, temperature of 50 deg.C) to obtain semen Scaphii Lychnophori extract with relative density (water density) of 1.3(60 deg.C).

(5) Mixing fructus Lycii and drinking water at a mass ratio of 1:20, soaking for 3 hr, heating and leaching at 80 deg.C, extracting twice, the first extraction for 2 hr and the second extraction for 1.5 hr, filtering while hot, mixing the two extractive solutions, centrifuging (20 deg.C, 4000r/min, 10min), concentrating under reduced pressure (steam pressure of 0.2MPa, temperature of 50 deg.C) to obtain fructus Lycii extract with relative density (water density of reference ratio) of 1.3(60 deg.C).

(6) Weighing 12g of maltodextrin, 60g of soluble starch, 0.01g of natural menthol, 1g of sodium carboxymethylcellulose, 0.1g of potassium sorbate and 0.1g of sodium citrate, and uniformly mixing to obtain auxiliary materials for later use.

(7) Weighing 4g of honeysuckle extract, 5g of chrysanthemum extract, 4g of dandelion extract, 5g of boat-fruited sterculia seed extract and 8g of medlar extract to obtain main medicine components for later use.

(8) Adding the main medicine components prepared in the step (7) into 74g of auxiliary materials, uniformly mixing the main medicine components, preparing into a held mass and soft materials which can be scattered by light pressing, extruding, sieving by a 16-mesh sieve to prepare into granular substances, drying the prepared granular substances in a drying box at 65 ℃ for 70min, collecting granules, sieving by a medicine detection sieve (the granules which pass through a No. 1 sieve but cannot pass through a No. 5 sieve), collecting and bagging.

The medicine taking method comprises the following steps: it is taken with warm water twice a day, 1.5g each time, and 15 days is a treatment course.

Example 2

In the step (6), the auxiliary materials comprise the following components: 12g of maltodextrin, 57g of soluble starch, 0.03g of natural menthol, 0.67g of sodium carboxymethyl cellulose, 0.1g of potassium sorbate and 0.2g of sodium citrate.

The main medicine components in the step (7) are as follows: 5g of honeysuckle extract, 5g of chrysanthemum extract, 5g of dandelion extract, 5g of boat-fruited sterculia seed extract and 10g of medlar extract.

70g of auxiliary materials in the step (8).

The rest of the procedure was the same as in example 1.

Example 3

In the step (6), the auxiliary materials comprise the following components: 12g of maltodextrin, 55g of soluble starch, 0.02g of natural menthol, 0.5g of sodium carboxymethylcellulose, 0.05g of potassium sorbate and 0.1g of sodium citrate

The main medicine components in the step (7) are as follows: 5g of honeysuckle extract, 6g of chrysanthemum extract, 5g of dandelion extract, 6g of boat-fruited sterculia seed extract and 10g of medlar extract.

68g of auxiliary materials in the step (8).

The rest of the procedure was the same as in example 1.

Example 4

In the step (6), the auxiliary materials comprise the following components: 11g of maltodextrin, 55g of soluble starch, 0.02g of natural menthol, 0.68g of sodium carboxymethyl cellulose, 0.1g of potassium sorbate and 0.2g of sodium citrate.

The main medicine components in the step (7) are as follows: 6g of honeysuckle extract, 5g of chrysanthemum extract, 6g of dandelion extract, 5g of boat-fruited sterculia seed extract and 11g of medlar extract.

67g of auxiliary materials in the step (8).

The rest of the procedure was the same as in example 1.

Example 5

In the step (6), the auxiliary materials comprise the following components: 10g of maltodextrin, 55g of soluble starch, 0.01g of natural menthol, 0.69g of sodium carboxymethyl cellulose, 0.1g of potassium sorbate and 0.2g of sodium citrate.

The main medicine components in the step (7) are as follows: 6g of honeysuckle extract, 6g of chrysanthemum extract, 6g of dandelion extract, 6g of boat-fruited sterculia seed extract and 10g of medlar extract.

66g of auxiliary materials in the step (8).

The rest of the procedure was the same as in example 1.

Example 6

In the step (6), the auxiliary materials comprise the following components: 10.66g of maltodextrin, 64g of soluble starch, 0.03g of natural menthol, 1g of sodium carboxymethyl cellulose, 0.1g of potassium sorbate and 0.2g of sodium citrate.

The main medicine components in the step (7) are as follows: 4g of honeysuckle extract, 4g of chrysanthemum extract, 4g of dandelion extract, 4g of boat-fruited sterculia seed extract and 8g of medlar extract.

76g of auxiliary materials in the step (8).

The rest of the procedure was the same as in example 1.

Comparative example 1

In the step (6), the auxiliary materials comprise the following components: 9g of maltodextrin, 50.5g of soluble starch, 0.07g of natural menthol, 0.13g of sodium carboxymethyl cellulose, 0.2g of potassium sorbate and 0.1g of sodium citrate.

In the step (7), the auxiliary materials comprise the following components: 3g of honeysuckle extract, 10g of chrysanthemum extract, 5g of dandelion extract, 7g of boat-fruited sterculia seed extract and 15g of medlar extract.

60g of auxiliary materials in the step (8).

The rest of the procedure was the same as in example 1.

Comparative example 2

Other technical features in this embodiment are the same as those in embodiment 1, and the granulation drying conditions are changed only to dry for 45min in a drying oven at 80 ℃, which is not repeated herein.

Comparative example 3

In the step (6), the auxiliary materials comprise the following components: 13g of maltodextrin, 56g of soluble starch, 0.02g of natural menthol, 0.78g of sodium carboxymethyl cellulose, 0.05g of potassium sorbate and 0.15g of sodium citrate.

In the step (7), the auxiliary materials comprise the following components: 6g of honeysuckle extract, 6g of dandelion extract, 6g of boat-fruited sterculia seed extract and 12g of medlar extract.

70g of auxiliary materials in the step (8).

The rest of the procedure was the same as in example 1.

Experiment 1 physical and chemical index detection

Subject: granulated teas prepared in example 1, example 2, example 3, example 4, example 5, example 6, comparative example 1, comparative example 2 and comparative example 3;

detection indexes are as follows: total number of bacterial colonies, pathogenic bacteria, lead, arsenic, moisture, copper, forming rate, moisture absorption rate and dissolubility;

the detection method comprises the following steps: the total number of colonies adopts a plate counting technology, pathogenic bacteria adopts a PCR technology, lead and copper adopt a flame atomic absorption spectrophotometry, arsenic adopts an atomic absorption spectrophotometry, moisture determination adopts a moisture meter SFY-20 series instrument, and dissolubility adopts a soluble particle inspection method;

the calculation method comprises the following steps:

molding ratio (%) — weight of particles that can pass through the first sieve and cannot pass through the fifth sieve/prepared particle weight × 100% moisture absorption ratio (%) (weight after moisture absorption-weight before moisture absorption)/weight before moisture absorption × 100%

The results are shown in Table 1.

TABLE 1 results of physical and chemical index measurements

The results in table 1 show that the granular tea prepared by the technical scheme of the invention is superior to the comparative examples in the aspects of forming rate, moisture absorption rate and dissolubility, and the formula and the process of the invention are reasonable.

Experiment 2 efficacy of in vitro simulated treatment of chronic pharyngitis

The bacterial detection rate of chronic pharyngitis in clinic is as follows: 54.4% of beta hemolytic streptococcus, 23.3% of staphylococcus aureus, 28.8% of klebsiella pneumoniae, 22.8% of staphylococcus epidermidis and the like. Therefore, the experiment examines the different degrees of inhibition effects of the honeysuckle extract, the chrysanthemum extract, the boat-fruited sterculia seed extract, the dandelion extract, the medlar extract and the compatible traditional Chinese medicine combined granulated tea in the embodiment 6 of the invention on the clinically common pathogenic microorganism bacterial suspension.

Subject: honeysuckle extract, chrysanthemum extract, sterculia scaphigera extract, dandelion extract, medlar extract and the granulated tea prepared in example 6, which are prepared in example 1;

detecting an object: minimum Inhibitory Concentrations (MIC) of Streptococcus hemolyticus B, Staphylococcus aureus, Klebsiella pneumoniae, and Staphylococcus epidermidis;

the experimental method comprises the following steps: test tube double dilution method;

the method comprises the following specific operations:

taking 5mL glass test tubes, and adding 2mL liquid culture medium (blood agar culture medium for beta hemolytic streptococcus; nutrient agar culture medium for Staphylococcus aureus, Klebsiella pneumoniae and Staphylococcus epidermidis) suitable for the bacterial suspension;

adding 2mL of honeysuckle extract into a 1 st tube of the marked honeysuckle, carefully blowing and uniformly mixing the honeysuckle extract by using a liquid-transferring gun, taking 2mL of the honeysuckle extract into a 2 nd tube of the marked honeysuckle, sequentially diluting the honeysuckle extract into 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128 and the like to a 10 th tube in a row concentration ratio, fully and uniformly mixing the marked honeysuckle extract in the 10 th tube, and discarding 2 mL;

taking the 11 th tube of the marked honeysuckle as a positive control group (aiming at observing the growth condition of bacteria);

fourthly, adding 2mL of honeysuckle extract into a 12 th pipe of the marked honeysuckle, uniformly mixing, discarding 2mL of liquid, and taking no bacterial suspension as negative control (aiming at observing whether the liquid medicine of the tested object is polluted or not);

fifthly, additionally setting a culture medium control experiment, and marking a 13 th tube without adding a liquid and a bacterial suspension in the test tube;

sixthly, adding the prepared bacterial suspension into a test tube marked with honeysuckle number 1-13a, uniformly mixing by blowing, and standing at 37 ℃ for constant-temperature culture for 20-24 h;

seventhly, similarly, test tubes 1-13b marked with chrysanthemum are filled with chrysanthemum extract, test tubes 1-13c marked with boat-fruited sterculia seed are filled with boat-fruited sterculia seed extract, test tubes 1-13d marked with dandelion are filled with dandelion extract, test tubes 1-13e marked with medlar are filled with medlar extract, and test tubes 1-13f marked with granular tea are filled with the compatible traditional Chinese medicine combined granular tea prepared solution in the embodiment 6 of the invention, and the operation is the same as above.

And (4) observing results: and (5) performing a plate streaking experiment, culturing for 24h, and observing the result to determine the minimum inhibitory concentration.

The results are shown in Table 2.

Table 2 in vitro simulation test results of clinical common pathogenic microorganism in each group

As can be seen from the results in Table 2, the positive control bacterial suspensions of each group grew well, and the negative control bacterial suspensions grew aseptically; the extract extracts have different degrees of inhibition effects on beta hemolytic streptococcus, staphylococcus aureus, klebsiella pneumoniae and staphylococcus epidermidis, and the minimum inhibition concentration of the traditional Chinese medicine composite granular tea in the example 6 is low, namely the dilution factor is maximum, compared with the extract extracts, so that the synergistic effect is achieved through reasonable proportion. The traditional Chinese medicine composite particle tea has broad-spectrum antibacterial effect.

Experiment 3 animal experiments

Subject: the SD rats weighing 180-200 g are 40 males and divided into 4 groups, and each group comprises 10 males;

grouping experiments: a blank control group, a model control group, a positive drug sarcandra glabra tablet group, and a traditional Chinese medicine combined granulated tea group (the traditional Chinese medicine combined granulated tea prepared in example 6);

the administration mode comprises the following steps: the blank control group is injected into the throat mucosa part of the mouse for the first time to give 4 mL-kg^-1The normal saline is changed into gastric lavage administration every day after the first administration; the model control group is given 4 mL/kg for the first time^-1The beta hemolytic streptococcus broth culture medium is injected into throat mucosa of mouse for molding, and is administered by intragastric administration for 4 mL/kg from the next day^-1The physiological saline of (4); the positive medicine sarcandra glabra tablet is injected with beta hemolytic streptococcus to make mould according to 0.6g kg^-1Intragastric administration of sarcandra glabra buccal tablet suspension; the weight of the Chinese medicinal granule tea group is 0.6 g/kg after being injected with the beta hemolytic streptococcus for molding^-1And (3) intragastrically administering the traditional Chinese medicine composition granular tea suspension.

The experimental method comprises the following steps: the rats are bred in cages in groups, water is freely drunk, administration is carried out for 15 days continuously, blood is taken from femoral artery to an Ep tube on the next day after the last administration, and then the rats in each group are killed by intraperitoneal injection of anesthetic.

The detection indexes and the method are as follows: measuring the content levels of Prostaglandin (PGE), interleukin-1 beta (1L-1 beta), tumor necrosis factor (TNF-alpha) by radioimmunoassay, and using the method according to the kit instructions;

the analysis method comprises the following steps: statistical analysis was performed using SPSS 22.0 statistical software, with the results expressed as mean ± standard deviation (x ± s), and with comparison using LSD-t test, significant differences were found when P <0.05 and very significant differences were found when P < 0.01.

The results of the experiment are shown in table 3.

TABLE 3 measurement results of content level

Group of	PGE(ng·L^-1)	1L-1β(ng·L^-1)	TNF-α(ng·L^-1)
				Blank control group	39.62±5.62	65.77±6.86	139.67±10.16
Model control group	56.7±7.46^#	92.87±8.86^#	263.58±18.26^#
				Group-containing sarcandra glabra tablet	49.66±6.11*	79.35±7.68*	217.63±13.16*
Traditional Chinese medicine combined granular tea set	42.80±5.17**	69.61±6.84**	143.85±13.16**

Note: comparison with model group (P < 0.05); comparison with model group (P < 0.01); comparison with blank group (P <0.05)

As shown in Table 3, PGE, 1L-1. beta. and TNF-. alpha.were significantly increased in the model control group compared with the blank control group, indicating that the molding was successful (P < 0.05). Compared with a blank control group, the content of the serum of the model control group is obviously increased, and the statistical significance is achieved, and compared with the model control group, the content of PGE, 1L-1 beta and TNF-alpha (P is less than 0.01) in the serum can be obviously reduced by the traditional Chinese medicine combined granular tea group; compared with the sarcandra glabra buccal tablet, the traditional Chinese medicine granule tea group is superior to the sarcandra glabra buccal tablet group. Therefore, the traditional Chinese medicine composite granular tea prepared by the invention has good anti-inflammatory effect on chronic pharyngitis.

Experiment 4 clinical experiment

First, general data of the experimental subjects: among 180 patients with chronic pharyngitis, 83 men and 97 women are aged 18-67 years old.

Clinical manifestations of the patients: pharyngeal discomfort, foreign body sensation, itching, burning sensation, dryness or irritation, with cough and nausea; the throat part is checked to see that mucous membrane is diffusely congested, dilated, dark red, accompanied by a small amount of viscous secretion; leucocytes and a small amount of lymphocytes infiltrate the blood vessels under the mucosa and around the mucosal glands, even the lymph follicles swell, often accompanied by yellowish white punctate exudates.

The treatment group and the control group are randomly divided, the treatment group comprises 90 cases, the control group comprises 90 cases, and the age, clinical condition and the like of the two groups have no obvious difference and are comparable.

Second, therapeutic methods

1. Control group: the compound sarcandra glabra buccal tablet is taken orally.

2. Treatment groups: the Chinese medicinal granule tea of the embodiment 6 of the invention is taken after being mixed with water.

Thirdly, usage and dosage:

1. control group

The usage and dosage are as follows: it is administered orally 2 tablets (small tablets) at a time, every 2 hr, 6 times daily.

2. Treatment group

The application method and the dosage are as follows: the preparation is taken twice a day, one dose is taken each time, and each dose contains 1.5g of the medicine, and is taken with warm water.

Fourthly, a course of treatment:

the course of treatment is as follows: 15 days is a course of treatment, and three courses of treatment are taken.

Fifth, observe the index

The improvement in clinical symptoms was recorded before and after treatment.

Sixthly, treatment course evaluation standard:

the main indexes for examining the normal chronic pharyngitis are as follows: the pharyngeal portion is checked to see that mucous membrane has no phenomena of diffuse hyperemia, vasodilatation and dark red color, and the viscous secretion disappears; there were no leukocytes and no lymphocytes infiltrating around the submucosal vessels and mucosal glands, the lymphoid follicles recovered normal, and the yellowish-white punctate exudates disappeared.

The effect is shown: pharyngeal obstruction, foreign body sensation, pharyngalgia, pharynx itch, burning sensation, dryness, irritation, cough, nausea, influence on phonation, etc. do not occur repeatedly, and the main index is normal.

The method has the following advantages: the occurrence of pharyngeal obstruction, foreign body sensation, pharyngalgia, pharynx itch, burning sensation, dryness, irritation, cough, nausea, influence on phonation, etc. is relieved, and the main indexes of the examination part are normal.

And (4) invalidation: pharyngeal obstruction, foreign body sensation, pharyngalgia, pharynx itch, burning sensation, dryness, irritation, cough, nausea, influence on phonation, etc., and no change in main index.

Seventh, therapeutic results

See table 4 for details.

TABLE 4 comparison of the curative effects of clinical symptoms in the patients of the treated and control groups

	Treatment group (90)	Percent (%)	Control group (90)	Percent (%)
					Show effect	45	50	33	37
Is effective	38	42	30	33
					Invalidation	7	8	27	30

As shown in table 4, the total effective rate of the treatment group was 92%, and the total effective rate of the control group was 70%. The traditional Chinese medicine granular tea has obvious curative effect on treating chronic pharyngitis and improves clinical symptoms of chronic pharyngitis.

The invention discloses a medicinal and edible traditional Chinese medicine combined granular tea for treating chronic pharyngitis and a preparation method thereof. The traditional Chinese medicine granular tea for treating chronic pharyngitis disclosed by the invention is convenient to drink, convenient to carry and safe to use, and the applied medicinal materials have natural food which is food and medicine, have the effects of health preservation and health care besides the function of definite curative effect, and are suitable for working crowds who use excessive voice for a long time, such as teachers, singers, salesmen and the like, and have a large market.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The traditional Chinese medicine composite granular tea for treating chronic pharyngitis is characterized by comprising the following components in parts by mass:

2. the traditional Chinese medicine composite granular tea for treating chronic pharyngitis according to claim 1, wherein the auxiliary materials comprise the following components in parts by mass:

3. the preparation method of the traditional Chinese medicine combined granular tea for treating chronic pharyngitis as claimed in claim 1 or 2, which is characterized by comprising the following steps:

4. The preparation method of the traditional Chinese medicine combined granular tea for treating chronic pharyngitis according to claim 3, wherein the step (1) is specifically operated as follows:

(13) respectively centrifuging the honeysuckle extract, the chrysanthemum extract, the dandelion extract, the sterculia lychnophora extract and the wolfberry fruit extract at 4000r/min at the temperature of 20-25 ℃ for 10min, and concentrating under reduced pressure (the steam pressure is 0.2MPa, and the temperature is 45-60 ℃) to the relative density of 1.2-1.4 to respectively obtain a honeysuckle extract, a chrysanthemum extract, a dandelion extract, a sterculia lychnophora extract and a wolfberry fruit extract.

5. The preparation method of the traditional Chinese medicine combined granular tea for treating chronic pharyngitis according to claim 4, wherein in the step (11), the superior mass material-liquid ratios of the honeysuckle, the dandelion and the wolfberry fruit to the drinking water are all 1 (15-20), and the superior mass material-liquid ratios of the chrysanthemum and the sterculia lychnophora to the drinking water are all 1 (20-25).

6. The method for preparing a herbal composition granule tea for treating chronic pharyngitis according to claim 4, wherein in the step (11), the preferred soaking time of honeysuckle, chrysanthemum, dandelion and sterculia lychnophora is 1-2 h, and the preferred soaking time of wolfberry is 2-3 h.

7. The method for preparing a herbal tea granule for treating chronic pharyngitis according to claim 4, wherein in the step (12), the preferred extraction temperatures for preparing the honeysuckle extract, the chrysanthemum extract, the dandelion extract and the boat-fruited sterculia seed extract are 90-100 ℃, and the preferred extraction temperature for preparing the wolfberry extract is 80-90 ℃.

8. The method for preparing a herbal tea granule for treating chronic pharyngitis according to claim 4, wherein in the step (13), the optimum centrifugation temperatures of the honeysuckle extract, the chrysanthemum extract, the dandelion extract and the sterculia lychnophora extract are all 25 ℃ and the optimum centrifugation temperature of the wolfberry extract is 20 ℃.

9. The preparation method of the traditional Chinese medicine combined granular tea for treating chronic pharyngitis according to claim 3, wherein the granular materials in the step (4) are dried in an oven at the temperature of 60-70 ℃ for 60-70 min.

10. The method for preparing the traditional Chinese medicine combined granular tea for treating chronic pharyngitis according to the claim 3, wherein the medicine screening in the step (4) is to collect dried granular substances which can pass through a No. 1 screen but cannot pass through a No. 5 screen, and then a finished product is obtained.