CN110559931B - Method and device for pneumatically mixing samples - Google Patents
Method and device for pneumatically mixing samples Download PDFInfo
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- CN110559931B CN110559931B CN201910962337.3A CN201910962337A CN110559931B CN 110559931 B CN110559931 B CN 110559931B CN 201910962337 A CN201910962337 A CN 201910962337A CN 110559931 B CN110559931 B CN 110559931B
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- 238000002156 mixing Methods 0.000 title claims abstract description 110
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 238000007789 sealing Methods 0.000 claims description 53
- 229920001971 elastomer Polymers 0.000 claims description 5
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000000338 in vitro Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 230000008859 change Effects 0.000 abstract description 4
- 238000003745 diagnosis Methods 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 238000003825 pressing Methods 0.000 description 4
- 238000009434 installation Methods 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 2
- 229920005372 Plexiglas® Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/401—Methods
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/403—Mixers using gas or liquid agitation, e.g. with air supply tubes for mixing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/408—Controlling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/4094—Plants
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
- Accessories For Mixers (AREA)
Abstract
The invention relates to the technical field of in-vitro diagnosis, in particular to a method for pneumatically mixing samples; the adopted technical scheme is as follows: the method comprises the following steps: placing the sample into a closed container; inserting two air ducts into the container, so that the outlet of one air duct is positioned above the liquid level of the sample, and the outlet of the other air duct is positioned below the liquid level of the sample; the two air ducts are alternately supplied with air to mix the samples uniformly, and the frequency of the alternate air supply of the air ducts is 5-15 Hz. The invention can realize the uniform mixing of the samples by only controlling the flowing direction of the gas, and the change of the gas direction can be realized by the electromagnetic directional valve. Therefore, the invention can realize high-frequency rapid mixing and has high mixing efficiency; in the mixing process, the sample is not in mechanical contact, so that the components or the structure of the sample are not damaged; meanwhile, the invention can uniformly mix samples which need to be stirred in a sealed environment.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnosis, in particular to a method and a device for pneumatically mixing samples.
Background
In the field of in vitro diagnostics, mixing samples is an essential step in the sample processing process. At present, two main ways of stirring samples to be tested for in-vitro diagnosis are: firstly, the sample and the sample reagent mixed solution are difficult to sufficiently mix by manually mixing through a stirring rod, and the efficiency is low and the labor is consumed; secondly, the stirring paddle is driven by the electric device to stir, and although the sample, the sample and the reagent mixed solution can be fully and uniformly mixed, the liquid is easy to splash and the sample components or the sample structure is easy to damage.
Disclosure of Invention
Aiming at the technical problems that the stirring and mixing efficiency of the sample is low, the mixing is insufficient and the sample is easy to damage during the existing in-vitro diagnosis, the invention provides a method for pneumatically mixing the sample, which can quickly and fully mix the sample without damaging the sample and can mix the sample in a closed container.
The invention is realized by the following technical scheme:
The method comprises the following steps:
Placing the sample into a closed container;
inserting two air ducts into the container, so that the outlet of one air duct is positioned above the liquid level of the sample, and the outlet of the other air duct is positioned below the liquid level of the sample;
The two air ducts are alternately supplied with air to mix the samples uniformly, and the frequency of the alternate air supply of the air ducts is 5-15 Hz.
When the samples are uniformly mixed, the air duct with the outlet below the liquid level is vented without applying pressure, and the air duct with the outlet above the liquid level of the samples is provided with air with certain pressure, so that the samples in the container enter the air duct with the outlet below the liquid level under the action of air pressure; then the air duct with the outlet positioned on the liquid level is vented without applying pressure, and the air duct with the outlet positioned under the liquid level of the sample is applied with gas with certain pressure, so that the sample returns to the container under the action of air pressure; the two air ducts are alternately supplied with air repeatedly, so that the aim of pneumatically mixing the samples is fulfilled.
The invention can realize the uniform mixing of the samples by only controlling the flowing direction of the gas, and the change of the gas direction can be realized by the electromagnetic directional valve. Therefore, the invention can realize high-frequency rapid mixing, has high mixing efficiency, and the mixing frequency can reach more than 10 times/second. And in the mixing process, the sample is not in mechanical contact, so that the components or structures of the sample are not damaged. Meanwhile, the invention can uniformly mix samples which need to be stirred in a sealed environment.
The invention also provides a device for pneumatically mixing samples, which is used for realizing the method:
The device comprises an open container, wherein a hollow mixing body is arranged in the container, a gap is reserved between the mixing body and the container, the upper end of the mixing body is open, the lower part of the mixing body is provided with an air outlet, and the air outlet is positioned at the lower part of the container; the container mouth of the container is adapted with a first sealing plug, the upper end of the mixing body is adapted with a second sealing plug, a gas channel is reserved between the first sealing plug and the second sealing plug, and the gas channel is communicated with the inside of the container. And filling the sample into the container so that the liquid level of the sample is higher than the air outlet, and alternately supplying air to the cavity between the mixing body and the second sealing plug, the interior of the container or the air channel to pneumatically mix the sample in the container.
Preferably, the volume of the upper part of the cavity of the mixing body is larger than the volume of the lower part, so that the contact area between the sample entering the mixing body and the inner wall of the mixing body can be enlarged, and the sample can be further ensured to be sufficiently mixed.
Preferably, a groove is arranged at the lower part of the mixing body, the air outlet is positioned at the bottom of the groove, and the impact of the sample on the mixing body during flowing is reduced through the groove, so that the integrity of the sample is ensured.
Preferably, the groove is spoon-shaped, and the air outlet is positioned in the middle of the bottom of the groove so as to further reduce the impact of the sample on the mixing body during flowing.
Optionally, a first air duct is inserted in the first sealing plug, one end of the first air duct is located outside the container, and the other end of the first air duct is located in the air channel; the second sealing plug is internally provided with a second air duct, one end of the second air duct is positioned in the cavity of the mixing body, and the other end of the second air duct passes through the first sealing plug and is provided with a container.
Optionally, the first sealing plug and the second sealing plug are made of rubber. The hollow needle head for medical use is convenient to pass through the sealing plug for air supply, so that air is supplied to the device when uniform mixing is required; after the mixing is completed, the needle head is pulled out to realize sealing so as to seal and store the mixed sample.
Preferably, the container is made of transparent materials, so that the mixing condition of the sample and the sucking position of the needle are conveniently observed.
Further, the container mouth is provided with a container cover, the container cover is used for plugging the container, the first sealing plug is positioned in the middle of the container cover, and the upper end of the mixing body is fixedly connected with the container cover so as to facilitate the installation and the disassembly of the mixing body.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. The invention can realize the uniform mixing of the samples by only controlling the flowing direction of the gas, and the change of the gas direction can be realized by the electromagnetic directional valve. Therefore, the invention can realize high-frequency rapid mixing, has high mixing efficiency, and the mixing frequency can reach more than 10 times/second. And in the mixing process, the sample is not in mechanical contact, so that the components or structures of the sample are not damaged. Meanwhile, the invention can uniformly mix samples which need to be stirred in a sealed environment.
2. The volume of the upper part of the cavity of the mixing body is larger than that of the lower part, so that the contact area between a sample entering the mixing body and the inner wall of the mixing body can be enlarged, and the sample can be fully mixed.
3. The lower part of the mixing body is provided with the spoon-shaped groove, and the air outlet is positioned in the middle of the bottom of the groove, so that the impact of a sample on the mixing body during flowing can be reduced, and the components or the structure of the sample can be ensured not to be damaged.
4. The first sealing plug and the second sealing plug are made of rubber. The hollow needle head for medical use is convenient to pass through the sealing plug for air supply, so that air is supplied to the device when uniform mixing is required; after the mixing is completed, the needle head is pulled out to realize sealing so as to seal and store the mixed sample.
Drawings
The accompanying drawings, which are included to provide a further understanding of embodiments of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the principles of the application. In the drawings:
fig. 1 is a schematic view of the structure of the device of the present invention.
Part names in the drawings:
1-container, 2-mixing body, 3-gas outlet, 4-first sealing plug, 5-second sealing plug, 6-gas channel, 7-recess, 8-first air duct, 9-second air duct, 10-container lid.
Detailed Description
For the purpose of making apparent the objects, technical solutions and advantages of the present invention, the present invention will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present invention and the descriptions thereof are for illustrating the present invention only and are not to be construed as limiting the present invention.
Example 1
A method of pneumatically blending a sample, comprising the steps of:
Placing the obtained proper amount of sample into a closed container, and adding the reagent into the container at the same time if the reagent is required to be added;
sealing the container, and inserting two air ducts into the container, so that the outlet of one air duct is positioned above the liquid level of the sample, and the outlet of the other air duct is positioned below the liquid level of the sample;
The two air ducts are alternately supplied with air by an air source (such as an air pump) and reversing elements such as reversing electromagnetic valves, so as to mix samples uniformly.
Wherein, the frequency of the alternating air supply of the air duct is 5-15 Hz, and the preferred embodiment is 10Hz.
When the samples are uniformly mixed, the air duct with the outlet below the liquid level is vented without applying pressure, and the air duct with the outlet above the liquid level of the samples is provided with air with certain pressure, so that the samples in the container enter the air duct with the outlet below the liquid level under the action of air pressure; then the air duct with the outlet positioned on the liquid level is vented without applying pressure, and the air duct with the outlet positioned under the liquid level of the sample is applied with gas with certain pressure, so that the sample returns to the container under the action of air pressure; the two air ducts are alternately supplied with air repeatedly, so that the aim of pneumatically mixing the samples is fulfilled.
The invention can realize the uniform mixing of the samples by only controlling the flowing direction of the gas, and the change of the gas direction can be realized by the electromagnetic directional valve. Therefore, the invention can realize high-frequency rapid mixing, has high mixing efficiency, and the mixing frequency can reach more than 10 times/second. And in the mixing process, the sample is not in mechanical contact, so that the components or structures of the sample are not damaged. Meanwhile, the invention can uniformly mix samples which need to be stirred in a sealed environment.
Example 2
An apparatus for pneumatically mixing samples for carrying out the method of embodiment 1 comprises an open vessel 1, wherein a hollow mixing body 2 is provided in the vessel 1. The mixing body 2 can be supported and fixed in the container 1 through the fixing plate, and the lower end of the mixing body 2 can also be directly fixedly connected with the interior of the container 1, and only a gap is reserved between the mixing body 2 and the container 1. The upper end of the mixing body 2 is provided with an opening, the lower part of the mixing body 2 is provided with an air outlet 3, and the air outlet 3 is positioned at the lower part of the container 1. The container 1 is provided with a first sealing plug 4 in a container opening, the upper end of the mixing body 2 is provided with a second sealing plug 5 in a matching mode, a gas channel 6 is reserved between the first sealing plug 4 and the second sealing plug 5, and the gas channel 6 is communicated with the inside of the container 1.
When the pneumatic mixing device is used, a sample is filled into the container, so that the liquid level of the sample is higher than the air outlet 3, and the air is alternately supplied to the cavity between the mixing body 2 and the second sealing plug 5, the inside of the container 1 or the air channel 6, so that the sample in the container can be pneumatically mixed uniformly. The device is typically supplied with air through a medical hollow needle passing through a sealing plug; it is also possible to supply air to the inside of the container 1 or the air passage 6 by providing an interface at the side wall of the container 1, and to provide a duct in the container 1 to communicate the cavity of the mixing body 2 with the outside of the container 1.
Preferably, the first sealing plug 4 and the second sealing plug 5 are made of rubber. The hollow needle head for medical use is convenient to pass through the sealing plug for air supply, so that air is supplied to the device when uniform mixing is required; after the mixing is completed, the needle head is pulled out to realize sealing so as to seal and store the mixed sample.
Further, the volume of the upper part of the cavity of the mixing body 2 is larger than the volume of the lower part. Compared with the cavity of the straight-through type mixing body 2, the contact area between the sample and the side wall of the cavity of the mixing body 2 can be increased under the same sample volume, so that the sample can be further mixed.
Preferably, a groove 7 is arranged at the lower part of the mixing body 2, and the air outlet 3 is positioned at the bottom of the groove 7. The grooves 7 reduce the impact of the sample on the mixing body 2 during the flow, so as to ensure the integrity of the sample. More preferably, the groove 7 is spoon-shaped, and the air outlet 3 is positioned in the middle of the bottom of the groove 7, namely, the air outlet 3 is arranged at the deepest part of the groove 7, so that the impact of the sample on the mixing body 2 can be further reduced.
Preferably, the container 1 is made of transparent material, such as polyurethane, plexiglas, glass, transparent PE, etc., so as to facilitate observation of the mixing condition of the sample and the drawing-in position of the needle.
Preferably, the container 1 is provided with a container cover 10 at the container mouth, the container cover 10 is used for plugging the container 1, and the first sealing plug 4 is positioned in the middle of the container cover 10. The upper end of the mixing body 2 is fixedly connected with the container cover 10, so that after the addition of the sample and the reagent is completed, the container cover 10 is covered to realize the installation of the mixing body 2, and the mixing body 2 can be taken out together by taking down the container cover 10, so that the installation and the disassembly of the mixing body 2 are convenient.
Example 3
Based on embodiment 2, the first sealing plug 4 is internally provided with a first air duct 8, one end of the first air duct 8 is located outside the container 1, and the other end of the first air duct 8 is located in the gas channel 6. That is, the first gas-guide tube 8 communicates the inside of the container 1 with the outside of the container 1, so that the inside of the container is easily supplied with gas. The second sealing plug 5 is internally provided with a second air duct 9, one end of the second air duct 9 is positioned in the cavity of the mixing body 2, and the other end of the second air duct 9 passes through the first sealing plug 4 and is provided with the container 1, namely, the second air duct 9 communicates the cavity inside the mixing body 2 with the outside of the container 1.
When the pneumatic air supply device is used, an air source is directly connected with the first air guide pipe 8 and the second air guide pipe 9, and air is alternately supplied through the reversing valve, so that a needle head and the like are not required to be inserted, and pneumatic mixing operation is facilitated. Before or after the mixing, the first air duct 8 and the second air duct 9 are sealed by rubber plugs, plugs and the like in the application occasions needing to store the samples in a sealing way.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (8)
1. A method of pneumatically blending a sample, comprising the steps of:
Placing the sample into a closed container;
inserting two air ducts into the container, so that the outlet of one air duct is positioned above the liquid level of the sample, and the outlet of the other air duct is positioned below the liquid level of the sample;
alternately supplying air to the two air ducts to mix the samples uniformly;
The mixing device comprises an open container (1), wherein a hollow mixing body (2) is arranged in the container (1), a gap is reserved between the mixing body (2) and the container (1), the upper end of the mixing body (2) is open, an air outlet (3) is arranged at the lower part of the mixing body (2), and the air outlet (3) is positioned at the lower part of the container (1); the container is characterized in that a first sealing plug (4) is adapted to a container opening of the container (1), a second sealing plug (5) is adapted to the upper end of the mixing body (2), a gas channel (6) is reserved between the first sealing plug (4) and the second sealing plug (5), and the gas channel (6) is communicated with the interior of the container (1);
A first air duct (8) is inserted in the first sealing plug (4), one end of the first air duct (8) is positioned outside the container (1), and the other end of the first air duct (8) is positioned in the air channel (6); the second sealing plug (5) is internally provided with a second air duct (9), one end of the second air duct (9) is positioned in the cavity of the mixing body (2), and the other end of the second air duct (9) penetrates through the first sealing plug (4) and is provided with a container (1).
2. The method for pneumatically mixing samples according to claim 1, wherein the frequency of alternate air supply of the air duct is 5-15 Hz.
3. A device for pneumatically mixing samples for implementing the method according to claim 1 or 2, comprising an open container (1), characterized in that: a hollow mixing body (2) is arranged in the container (1), a gap is reserved between the mixing body (2) and the container (1), the upper end of the mixing body (2) is opened, an air outlet (3) is arranged at the lower part of the mixing body (2), and the air outlet (3) is positioned at the lower part of the container (1); the container is characterized in that a first sealing plug (4) is adapted to a container opening of the container (1), a second sealing plug (5) is adapted to the upper end of the mixing body (2), a gas channel (6) is reserved between the first sealing plug (4) and the second sealing plug (5), and the gas channel (6) is communicated with the interior of the container (1);
A first air duct (8) is inserted in the first sealing plug (4), one end of the first air duct (8) is positioned outside the container (1), and the other end of the first air duct (8) is positioned in the air channel (6); the second sealing plug (5) is internally provided with a second air duct (9), one end of the second air duct (9) is positioned in the cavity of the mixing body (2), the other end of the second air duct (9) penetrates through the first sealing plug (4) to be provided with the outside of the container (1), and the volume of the upper part of the cavity of the mixing body (2) is larger than that of the lower part.
4. A device for pneumatically mixing samples as defined in claim 3, wherein: the lower part of the mixing body (2) is provided with a groove (7), and the air outlet (3) is positioned at the bottom of the groove (7).
5. The apparatus for pneumatically mixing samples as recited in claim 4, wherein: the groove (7) is spoon-shaped, and the air outlet (3) is positioned in the middle of the bottom of the groove (7).
6. A device for pneumatically mixing samples as defined in claim 3, wherein: the first sealing plug (4) and the second sealing plug (5) are made of rubber.
7. The apparatus for pneumatically mixing samples as recited in claim 6, wherein: the container (1) is made of transparent material.
8. A device for pneumatically mixing samples as defined in claim 3, wherein: the container is characterized in that a container cover (10) is arranged at a container opening of the container (1), the container cover (10) is used for sealing the container (1), the first sealing plug (4) is located in the middle of the container cover (10), and the upper end of the mixing body (2) is fixedly connected with the container cover (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910962337.3A CN110559931B (en) | 2019-10-11 | 2019-10-11 | Method and device for pneumatically mixing samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910962337.3A CN110559931B (en) | 2019-10-11 | 2019-10-11 | Method and device for pneumatically mixing samples |
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| Publication Number | Publication Date |
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| CN110559931A CN110559931A (en) | 2019-12-13 |
| CN110559931B true CN110559931B (en) | 2024-05-28 |
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| CN201910962337.3A Active CN110559931B (en) | 2019-10-11 | 2019-10-11 | Method and device for pneumatically mixing samples |
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| CN112795433B (en) * | 2021-03-19 | 2021-07-27 | 江西天佳生物工程股份有限公司 | Mixing apparatus of plant essential oil |
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|---|---|---|---|---|
| JPH09192475A (en) * | 1996-01-23 | 1997-07-29 | Terametsukusu Kk | Liquid pressurizing vessel |
| CN201607358U (en) * | 2009-12-11 | 2010-10-13 | 上海快德行生物技术有限公司 | Mixing device of medical testing sample |
| CN103439138A (en) * | 2013-09-13 | 2013-12-11 | 四川沃文特生物技术有限公司 | Liquid adding, even-mixing, sampling and pretreating integrated machine and realizing method thereof |
| CN103439139A (en) * | 2013-09-13 | 2013-12-11 | 四川沃文特生物技术有限公司 | Disposable specimen collecting tube and applying method thereof |
| CN210699886U (en) * | 2019-10-11 | 2020-06-09 | 四川沃文特生物技术有限公司 | Pneumatic device for mixing samples |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6780160B2 (en) * | 2002-07-25 | 2004-08-24 | Akfa Scientific Designs, Inc. | Specimen collection and application apparatus |
-
2019
- 2019-10-11 CN CN201910962337.3A patent/CN110559931B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09192475A (en) * | 1996-01-23 | 1997-07-29 | Terametsukusu Kk | Liquid pressurizing vessel |
| CN201607358U (en) * | 2009-12-11 | 2010-10-13 | 上海快德行生物技术有限公司 | Mixing device of medical testing sample |
| CN103439138A (en) * | 2013-09-13 | 2013-12-11 | 四川沃文特生物技术有限公司 | Liquid adding, even-mixing, sampling and pretreating integrated machine and realizing method thereof |
| CN103439139A (en) * | 2013-09-13 | 2013-12-11 | 四川沃文特生物技术有限公司 | Disposable specimen collecting tube and applying method thereof |
| CN210699886U (en) * | 2019-10-11 | 2020-06-09 | 四川沃文特生物技术有限公司 | Pneumatic device for mixing samples |
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