CN110408524A - A kind of sampler of bioreactor - Google Patents
A kind of sampler of bioreactor Download PDFInfo
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- CN110408524A CN110408524A CN201910745226.7A CN201910745226A CN110408524A CN 110408524 A CN110408524 A CN 110408524A CN 201910745226 A CN201910745226 A CN 201910745226A CN 110408524 A CN110408524 A CN 110408524A
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- sampling bottle
- aspiration pump
- microprocessor
- probe tube
- bioreactor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/07—Dosage or metering devices therefore
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to biological medicine experimental tool fields, specifically disclose a kind of sampler of bioreactor, its sampling bottle upper end is communicated with probe tube, and probe tube includes for protruding into half probe tube of the right side sampled in bioreactor, and the lower end of right half probe tube is equipped with solenoid valve and liquid level sensor；The upper end of sampling bottle is equipped with aspiration pump and microprocessor, and wherein aspiration pump is connected to sampling bottle, and aspiration pump is connect with aspiration pump control switch signal；The lateral wall of sampling bottle is equipped with display screen, and the inner sidewall upper end of sampling bottle is equipped with level detection sensors device, and sampling bottle upper end is additionally provided with vent valve, and sampling bottle lower end is equipped with culture solution bleeder valve；Microprocessor is connect with liquid level sensor, aspiration pump control switch, level detection sensors device, display screen, solenoid valve and deflation valve signal respectively.The present invention can accurately different depth carries out culture solution sampling in the bioreactor, studies so as to the biological inoculum targetedly to different depth position in bioreactor.
Description
Technical field
The present invention relates to biological medicine experimental tool field, in particular to a kind of sampler of bioreactor.
Background technique
It is indispensable to the culture of biological inoculum in Bioexperiment, it is deep to the difference in the culture apparatus such as bioreactor
Biological inoculum at degree position is furtherd investigate, and is an important link in biological inoculum culture.In the bioreactor
It needs to be sampled biological sample after the completion of culture, biological inoculum is liquid mostly, is used in major part experimentation at present
Liquid getting device samples, can not easily be different in culture solution although such liquid getting device has the characteristics that sampling is convenient
Depth be accurately sampled, therefore can not fully understand the biological inoculum culture shape of different location in bioreactor
Condition.
Summary of the invention
The purpose of the present invention is overcoming above-mentioned problems of the prior art, a kind of sampling dress of bioreactor is provided
It sets, can accurately carry out culture solution sampling at different depth in the bioreactor, so as to targetedly anti-to biology
The biological inoculum of different depth position in device is answered to be studied.
The technical scheme is that a kind of sampler of bioreactor, including sampling bottle, the sampling bottle upper end
Side wall on be communicated with probe tube, the probe tube includes half probe tube of left side inside sampling bottle and outside sampling bottle
Portion is used to protrude into half probe tube of the right side being sampled in bioreactor, and the lower inlet of right half probe tube is equipped with electromagnetism
The lower end outside wall of valve, right half probe tube is equipped with liquid level sensor；The upper end of the sampling bottle is equipped with accommodating case, in accommodating case
It is respectively equipped with aspiration pump, microprocessor and power module, wherein aspiration pump is connected to sampling bottle, for aspirating in sampling bottle
Air makes to form negative pressure in sampling bottle, and aspiration pump is connect with aspiration pump control switch signal, on the accommodating case lateral wall respectively
Master switch and the aspiration pump control switch are set；The lateral wall of the sampling bottle is equipped with display screen, the inside of sampling bottle
Wall upper end is equipped with level detection sensors device, and the upper end of sampling bottle is additionally provided with vent valve, and the lower end of sampling bottle is equipped with culture solution tapping
Valve；The microprocessor respectively with solenoid valve, liquid level sensor, aspiration pump control switch, level detection sensors device, display screen,
Vent valve and the connection of master switch signal；The aspiration pump, display screen and microprocessor are also electrically connected with power module respectively；
The master switch is for power supply of the power module to aspiration pump, display screen and microprocessor described on and off；The pumping
Air pump control switch controls the opening and closing of aspiration pump for microprocessor by it；The liquid level sensor is for real-time measurement right half
Probe tube protrudes into the medium depth value in bioreactor, and measured depth value is sent to the micro process in real time
Device；The microprocessor is arranged with depth bounds in real time for depth value described in real-time reception, and by received depth value
Value is compared, when received depth value is located within the scope of depth bounds setting value, described in the microprocessor control
Vent valve is in close state, and microprocessor controls the aspiration pump by the aspiration pump control switch and opens, and carries out
Pumping, while microprocessor controls the solenoid valve and opens, and draws to the culture solution in bioreactor；Work as microprocessor
Not when within depth bounds setting value, the microprocessor is controlled the depth value received by the aspiration pump control switch
The aspiration pump is closed, and stops pumping, and microprocessor controls the solenoid valve and closes, while described in microprocessor also controls
Vent valve unlatching is deflated, so that ambient atmosphere enters in the sampling bottle；The level detection sensors device for examining in real time
Whether the liquid level surveyed in the sampling bottle has reached level detection sensors device position, when detecting that the liquid level has reached liquid
When the detection sensor position of face, level detection sensors device, which sends sampling bottle to the microprocessor, has expired signal, micro process
Device receives after the sampling bottle expired signal, passes through the aspiration pump control switch and controls the aspiration pump and close, stops taking out
Gas；The display screen is used for liquid described in real-time display for the depth bounds setting value to be arranged by way of touch
Depth value measured by level sensor.
Above-mentioned left half probe tube is located inside sampling bottle and protrudes into sampling bottle bottom；The upper end of left half probe tube is worn
The side wall of sampling bottle out is sealed and is fixedly connected between sampling bottle side wall, is sealed between left half probe tube and right half probe tube
And it is detachably connected.
Be additionally provided on the lower end outside wall of above-mentioned right half probe tube temperature sensor for measuring culture-liquid temp and
For shooting the camera of video image in culture solution；The display screen is also used to measured by temperature sensor described in real-time display
Culture-liquid temp value and camera captured by video image in culture solution.
Handle is additionally provided on the side wall of above-mentioned sampling bottle.
Above-mentioned sampling bottle is clear vial, and the scale value for reading liquid level in bottle is additionally provided on the side wall of sampling bottle.
Above-mentioned right half probe tube is made of the telescopic sleeve that multistage connects together, and the multistage connect together it is flexible
Rubber hose is equipped in casing, the lower end of rubber hose is connected to the solenoid valve, the upper end of rubber hose and left half probe tube
Connection.
Above-mentioned microprocessor is PLC controller or MSP430 single-chip microcontroller.
It, being capable of accurate different depth in the bioreactor the present invention provides a kind of sampler of bioreactor
Place carries out culture solution sampling, grinds so as to the biological inoculum targetedly to different depth position in bioreactor
Study carefully.Depth selection accuracy of the present invention is high, sampling process automation, and when deviateing sampling setting depth, can be automatically stopped
Sampling, sampling can be automatically continued when reaching depth selection again, sampling can be automatically stopped after sampling bottle is filled.The present invention takes
Sample intelligence degree is high, and easy to operate, sampling process is more time saving and energy saving, realizes one-touch sampling operation.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is that electric system of the invention connects block diagram.
Specific embodiment
With reference to the accompanying drawing, the specific embodiment of the present invention is described in detail, it is to be understood that of the invention
Protection scope be not limited by the specific implementation.
It is described the present invention provides a kind of sampler of bioreactor, including sampling bottle 1 referring to such as Fig. 1 and Fig. 2
Probe tube 2 is communicated on the side wall of 1 upper end of sampling bottle, the probe tube 2 includes the half probe tube 2- of a left side inside sampling bottle 1
1 and outside sampling bottle 1 for protruding into the half probe tube 2-2 of the right side being sampled in bioreactor, the right side partly takes
The lower inlet of sample pipe 2-2 is equipped with solenoid valve 13, and the lower end outside wall of half probe tube 2-2 of the right side is equipped with liquid level sensor 17；Institute
The upper end for stating sampling bottle 1 is equipped with accommodating case 6, is respectively equipped with aspiration pump 3, microprocessor 4 and power module 5 in accommodating case 6,
Middle aspiration pump 3 is connected to sampling bottle 1, for aspirating the air in sampling bottle 1, makes to form negative pressure in sampling bottle 1, aspiration pump 3 with
8 signal of aspiration pump control switch connects, and master switch 7 and aspiration pump control are respectively set on 6 lateral wall of accommodating case
Switch 8；The lateral wall of the sampling bottle 1 is equipped with display screen 10, and the inner sidewall upper end of sampling bottle 1 is equipped with level detection sensors device
11, the upper end of sampling bottle 1 is additionally provided with vent valve 9, and the lower end of sampling bottle 1 is equipped with culture solution bleeder valve 14；The microprocessor 4 divides
Not with solenoid valve 13, liquid level sensor 17, aspiration pump control switch 8, level detection sensors device 11, display screen 10, vent valve 9 with
And 7 signal of master switch connection；The aspiration pump 3, display screen 10 and microprocessor 4 are also electrically connected with power module 5 respectively；Institute
Master switch 7 is stated for power supply of the power module 5 to aspiration pump 3, display screen 10 and microprocessor 4 described on and off；Institute
State the opening and closing that aspiration pump control switch 8 controls aspiration pump 3 for microprocessor 4 by it；The liquid level sensor 17 is for real
When measurement half probe tube 2-2 of the right side protrude into the medium depth value in bioreactor, and measured depth value is sent in real time
To the microprocessor 4；The microprocessor 4 is used for depth value described in real-time reception, and received depth value is real-time
It is compared with depth bounds setting value, it is described micro- when received depth value is located within the scope of depth bounds setting value
Processor 4 controls the vent valve 9 and is in close state, and microprocessor 4 controls institute by the aspiration pump control switch 8
The unlatching of aspiration pump 3 is stated, is evacuated, while microprocessor 4 controls the solenoid valve 13 and opens, to the culture in bioreactor
Liquid is drawn；When the depth value that microprocessor 4 receives is not when within depth bounds setting value, the microprocessor 4 is logical
It crosses the aspiration pump control switch 8 and controls the closing of aspiration pump 3, stop pumping, and microprocessor 4 controls the solenoid valve
13 close, while microprocessor 4 also controls the unlatching of vent valve 9 and deflates, so that ambient atmosphere enters the sampling bottle 1
It is interior；Whether liquid level of the level detection sensors device 11 in sampling bottle 1 described in real-time detection has reached level detection sensors
11 position of device, when detecting that the liquid level has reached level detection sensors 11 position of device, level detection sensors device
11, which send sampling bottle to the microprocessor 4, has expired signal, and microprocessor 4 receives after the sampling bottle expired signal, passes through
The aspiration pump control switch 8 controls the aspiration pump 3 and closes, and stops pumping；The display screen 10 is used for the side by touching
The depth bounds setting value is arranged in formula, while for depth value measured by liquid level sensor 17 described in real-time display.This reality
Apply example can accurately in the bioreactor different depth carry out culture solution sampling, so as to targetedly to biological respinse
The biological inoculum of different depth position is studied in device.Depth selection accuracy of the present invention is high, sampling process automation, and
When deviateing sampling setting depth, it can be automatically stopped sampling, sampling can be automatically continued when reaching depth selection again, works as sampling
Bottled Man Houhui is automatically stopped sampling.
Further, the left half probe tube 2-1 is located at the inside of sampling bottle 1 and protrudes into 1 bottom of sampling bottle, by by left half
Probe tube 2-1 protrudes into 1 bottom of sampling bottle, can prevent culture solution during sampling from splashing in bottle civil strike, so that sampling process is flat
Surely；The upper end of the left half probe tube 2-1 is pierced by the side wall of sampling bottle 1, sealing and fixed company between 1 side wall of sampling bottle
It connects, seals and be detachably connected between half probe tube 2-2 of half probe tube 2-1 of a left side and the right side, it, can be with by the structure being detachably connected
Easily unloads half probe tube 2-2 of the right side down and it is cleaned, stowing operation.
Further, the temperature for measuring culture-liquid temp is additionally provided on the lower end outside wall of the right half probe tube 2-2
Spend sensor 16 and the camera 15 for shooting video image in culture solution；The display screen 10 is also used to real-time display institute
State the video image in culture solution captured by culture-liquid temp value and camera 15 measured by temperature sensor 16.Pass through
Temperature sensor 16 and camera 15 can understand temperature and culture solution in culture solution at different depth position in sampling
The situations such as turbidity.
Further, it is additionally provided with handle 12 on the side wall of the sampling bottle 1, setting handle 12 can conveniently take this sampling
Device is sampled operation.
Further, the sampling bottle 1 is clear vial, is additionally provided on the side wall of sampling bottle 1 for reading liquid level in bottle
Scale value 18 can accurately be determined sampling amount by scale value 18, when sampling amount reaches required amount, as long as to
Upper extraction apparatus of the present invention, can be automatically stopped sampling process.
Further, the right half probe tube 2-2 is made of the telescopic sleeve that multistage connects together, and the multistage is socketed
Rubber hose is equipped in telescopic sleeve together, the lower end of rubber hose is connected to the solenoid valve 13, rubber hose it is upper
End is connected to half probe tube 2-1 of a left side.Half probe tube 2-2 of the right side is set as telescopic fashion and facilitates its storage, avoids its too long institute
Caused by disadvantage not easy to store.
Further, the microprocessor 4 is the ripening electrons component such as PLC controller or MSP430 single-chip microcontroller, such portion
Part market is common, is easy to buy, and stable working performance.
In conclusion a kind of sampler of bioreactor provided by the invention, a kind of sampling dress of bioreactor
Set, can accurately in the bioreactor different depth carry out culture solution sampling, so as to targetedly to biological respinse
The biological inoculum of different depth position is studied in device.Depth selection accuracy of the present invention is high, sampling process automation, and
When deviateing sampling setting depth, it can be automatically stopped sampling, sampling can be automatically continued when reaching depth selection again, works as sampling
Bottled Man Houhui is automatically stopped sampling.The present invention samples intelligence degree height, and easy to operate, sampling process is more time saving and energy saving, real
One-touch sampling operation is showed.
Disclosed above is only several specific embodiments of the invention, and still, the embodiment of the present invention is not limited to this, is appointed
What what those skilled in the art can think variation should all fall into protection scope of the present invention.
Claims (7)
1. a kind of sampler of bioreactor, including sampling bottle (1), it is communicated on the side wall of sampling bottle (1) upper end
Probe tube (2), which is characterized in that the probe tube (2) include positioned at sampling bottle (1) internal half probe tube of a left side (2-1) and
Positioned at sampling bottle (1) outside for protruding into half probe tube of the right side (2-2) being sampled in bioreactor, described right half is sampled
The lower inlet for managing (2-2) is equipped with solenoid valve (13), and the lower end outside wall of right half probe tube (2-2) is equipped with liquid level sensor
(17)；The upper end of the sampling bottle (1) is equipped with accommodating case (6), is respectively equipped with aspiration pump (3), microprocessor in accommodating case (6)
(4) and power module (5), wherein aspiration pump (3) is connected to sampling bottle (1), for aspirating the air in sampling bottle (1), is made
Negative pressure is formed in sampling bottle (1), aspiration pump (3) is connect with aspiration pump control switch (8) signal, accommodating case (6) lateral wall
On master switch (7) and the aspiration pump control switch (8) is respectively set；The lateral wall of the sampling bottle (1) is equipped with display
Shield (10), the inner sidewall upper end of sampling bottle (1) is equipped with level detection sensors device (11), and the upper end of sampling bottle (1) is additionally provided with deflation
The lower end of valve (9), sampling bottle (1) is equipped with culture solution bleeder valve (14)；The microprocessor (4) respectively with solenoid valve (13), liquid
Level sensor (17), aspiration pump control switch (8), level detection sensors device (11), display screen (10), vent valve (9) and total
Switch the connection of (7) signal；The aspiration pump (3), display screen (10) and microprocessor (4) are also electric with power module (5) respectively
Connection；
The master switch (7) is used for power module (5) described on and off to aspiration pump (3), display screen (10) and micro- place
Manage the power supply of device (4)；The aspiration pump control switch (8) controls the opening and closing of aspiration pump (3) for microprocessor (4) by it；
The liquid level sensor (17) protrudes into the medium depth in bioreactor for right half probe tube (2-2) of real-time measurement
Value, and measured depth value is sent to the microprocessor (4) in real time；
The microprocessor (4) is for depth value described in real-time reception, and in real time and depth bounds by received depth value
Setting value is compared, when received depth value is located within the scope of depth bounds setting value, microprocessor (4) control
It makes the vent valve (9) to be in close state, and microprocessor (4) passes through described in the aspiration pump control switch (8) control
Aspiration pump (3) is opened, and is evacuated, while microprocessor (4) controls the solenoid valve (13) and opens, in bioreactor
Culture solution is drawn；When the depth value that microprocessor (4) receives is not when within depth bounds setting value, the micro process
Device (4) controls the aspiration pump (3) by the aspiration pump control switch (8) and closes, and stops pumping, and microprocessor (4)
The solenoid valve (13) closing is controlled, while microprocessor (4) also controls the vent valve (9) unlatching and deflates, so that outside
Boundary's atmosphere enters in the sampling bottle (1)；
Whether liquid level of the level detection sensors device (11) in sampling bottle (1) described in real-time detection has reached level detection
Sensor (11) position, when detecting that the liquid level has reached level detection sensors device (11) position, liquid level inspection
Surveying sensor (11) Xiang Suoshu microprocessor (4) transmission sampling bottle has expired signal, and microprocessor (4) has received the sampling bottle
After full signal, the aspiration pump (3) is controlled by the aspiration pump control switch (8) and is closed, pumping is stopped；
The display screen (10) is used for real-time display institute for the depth bounds setting value to be arranged by way of touch
State depth value measured by liquid level sensor (17).
2. a kind of sampler of bioreactor as described in claim 1, which is characterized in that the left half probe tube (2-
1) it is located at sampling bottle (1) inside and protrudes into sampling bottle (1) bottom；The upper end of left half probe tube (2-1) is pierced by sampling bottle
(1) side wall is sealed and is fixedly connected between sampling bottle (1) side wall, left half probe tube (2-1) and right half probe tube (2-2)
Between seal and be detachably connected.
3. a kind of sampler of bioreactor as described in claim 1, which is characterized in that the right half probe tube (2-
2) temperature sensor (16) for measuring culture-liquid temp is additionally provided on lower end outside wall and for shooting in culture solution
The camera (15) of video image；The display screen (10) is also used to training measured by temperature sensor described in real-time display (16)
Video image in culture solution captured by nutrient solution temperature value and camera (15).
4. a kind of sampler of bioreactor as described in claim 1, which is characterized in that the side of the sampling bottle (1)
Handle (12) are additionally provided on wall.
5. a kind of sampler of bioreactor as described in claim 1, which is characterized in that the sampling bottle (1) is
Bright bottle is additionally provided with the scale value (18) for reading liquid level in bottle on the side wall of sampling bottle (1).
6. a kind of sampler of bioreactor as described in claim 1, which is characterized in that the right half probe tube (2-
2) it is made of the telescopic sleeve that multistage connects together, and is equipped with rubber hose in the telescopic sleeve that connects together of the multistage,
The lower end of rubber hose is connected to the solenoid valve (13), and the upper end of rubber hose is connected to left half probe tube (2-1).
7. a kind of sampler of bioreactor as described in claim 1, which is characterized in that the microprocessor (4) is
PLC controller or MSP430 single-chip microcontroller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910745226.7A CN110408524B (en) | 2019-08-13 | 2019-08-13 | Sampling device of bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910745226.7A CN110408524B (en) | 2019-08-13 | 2019-08-13 | Sampling device of bioreactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110408524A true CN110408524A (en) | 2019-11-05 |
| CN110408524B CN110408524B (en) | 2022-09-09 |
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ID=68367185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910745226.7A Active CN110408524B (en) | 2019-08-13 | 2019-08-13 | Sampling device of bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110408524B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112629934A (en) * | 2020-12-30 | 2021-04-09 | 富士胶片电子材料（苏州）有限公司 | Hundred-grade cleanliness sampling cabinet |
| CN114486360A (en) * | 2022-01-25 | 2022-05-13 | 江苏徐塘发电有限责任公司 | Multifunctional sampling device and control method thereof |
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| US20070128087A1 (en) * | 2003-08-22 | 2007-06-07 | Ismatec Sa, Laboratoriumstechnik | Device for automated bioreactor sampling |
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| CN108844584A (en) * | 2018-07-02 | 2018-11-20 | 合肥赑歌数据科技有限公司 | A kind of device for the dynamic layered environmental monitoring of farmland water quality |
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| GB876975A (en) * | 1959-02-16 | 1961-09-06 | Atomic Energy Authority Uk | Improvements in or relating to sampling devices |
| US20070128087A1 (en) * | 2003-08-22 | 2007-06-07 | Ismatec Sa, Laboratoriumstechnik | Device for automated bioreactor sampling |
| CN206986178U (en) * | 2017-07-28 | 2018-02-09 | 栗帅 | A kind of sampler of new-type bioreactor |
| CN207717422U (en) * | 2018-01-09 | 2018-08-10 | 广东微碳检测科技有限公司 | New type of deep-water sampler for environment measuring |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112629934A (en) * | 2020-12-30 | 2021-04-09 | 富士胶片电子材料（苏州）有限公司 | Hundred-grade cleanliness sampling cabinet |
| CN114486360A (en) * | 2022-01-25 | 2022-05-13 | 江苏徐塘发电有限责任公司 | Multifunctional sampling device and control method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110408524B (en) | 2022-09-09 |
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