CN110550744A - Application of pseudomonas with algae-lysing activity - Google Patents
Application of pseudomonas with algae-lysing activity Download PDFInfo
- Publication number
- CN110550744A CN110550744A CN201910900731.4A CN201910900731A CN110550744A CN 110550744 A CN110550744 A CN 110550744A CN 201910900731 A CN201910900731 A CN 201910900731A CN 110550744 A CN110550744 A CN 110550744A
- Authority
- CN
- China
- Prior art keywords
- algae
- pseudomonas
- chlorella
- water body
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses an application of pseudomonas with algae-lysing activity, belonging to the technical field of microbial algae control, wherein the preservation number of the pseudomonas is CGMCC No.8398, and the pseudomonas is preserved in the common microorganism center of China Committee for culture Collection of microorganisms; the bacterial cells of the Pseudomonas fragi X11 have strong algae dissolving effect on chlorella, when the volume ratio of algae to bacteria is 4:1, the algae dissolving rate can reach more than 85 percent, and the water body mainly suffering from eutrophication due to chlorella outbreak can be effectively treated; the chlorella culture medium is used for resuspending the fermentation bacteria cells, and the chlorella culture medium is used for dissolving algae and is attached to the water body environment with actual eutrophication.
Description
Technical Field
The invention relates to the technical field of microbial algae control, in particular to application of pseudomonas with algae-lysing activity.
Background
in recent years, with the rapid advance of industrialization and urbanization and the increasing discharge amount, the eutrophication of the lake water environment is becoming more and more serious. The eutrophication of water bodies generally refers to the process of enriching nitrogen and phosphorus nutrient elements in water bodies such as lakes, reservoirs, gulfs and the like, reducing the dissolved oxygen amount of the water bodies, deteriorating the water quality and losing the self-purification function of the water bodies. One of the hazards is that some characteristic algae (mainly blue algae and green algae) are abnormally proliferated, fishes and other organisms are massively killed, and the aquatic ecological environment is seriously damaged.
In order to solve the environmental problem that eutrophication of water body is becoming more serious and algae frequently explodes, researchers at home and abroad propose corresponding prevention and treatment methods which can be mainly divided into three major types, namely physical, chemical and biological methods. The physical method mainly means that the algae in the water body is rapidly removed by means of manpower, instruments, equipment or mechanical devices and the like, such as sediment dredging, mechanical fishing, air flotation, ultrasonic algae removal and the like. The method has a small application range, can only treat small areas for a short time, has certain limitation and can not thoroughly eradicate algae and the negative effects caused by the algae. The chemical method is to treat water eutrophication problem with chemical medicine or mineral matter with obvious algae inhibiting effect, such as adding copper sulfate, sodium hypochlorite, hydrogen peroxide, surfactant, etc. The chemical reagents in this method pose potential, unknown hazards to aquatic ecosystems, and in addition, are costly to use and cause serious secondary pollution, thus limiting their use. Compared with the physical and chemical method, the method has the characteristics of species specificity, low cost, environmental friendliness, stable operability and the like, and can fundamentally and thoroughly solve the problem of algae outbreak. Therefore, most scholars in recent years are dedicated to research on new and more effective biological methods for treating eutrophication of water bodies.
The method for treating the algae erupted in the eutrophic water body by utilizing the algicidal bacteria is the most widely applied and effective algae removal method. Algicidal bacteria are a class of microorganisms that selectively inhibit, kill, and lyse algal cells by direct contact or secretion of allelochemicals. It is attracting great attention in controlling abnormal proliferation of algae in water and regulating the balance of aquatic ecological system. The algicidal bacteria have been reported mainly to include Bacillus (Bacillus), Alternaria (Alternamonas), Cellulomonas (Cellvibrio), Pseudomonas (Pseudomonas), Hahela (Hahella chejuensis), Alternaria (Pseudomonas), Staphylococcus (Staphylococus), Vibrio (Vibrio) and the like. In addition, the CFB flora (Cytophaga-Flavobacterium-Bacteroides, Cytophaga-Flarobacterium-bacteriodes) is considered as another flora containing various algicidal bacteria. Currently, the bacterium which is reported to be capable of dissolving algae most in Pseudomonas is Pseudomonas aeruginosa, while the application of the algae-dissolving property of Pseudomonas fragi is not reported, and the application of Pseudomonas in the aspect of algae control in eutrophic water bodies is still in the initial stage.
Most algae in eutrophic water body belong to blue algae and green algae, most algae-dissolving bacteria separated at home and abroad are directed at the blue algae in the water body, wherein the blue algae mainly comprises microcystis, anabaena, oscillatoria and the like, while the research on the algae-dissolving of another dominant algae species green algae in the water bloom is less, wherein the green algae mainly comprises chlorella, scenedesmus, and the like. The chlorella is a single-cell eukaryotic microalgae, has wide distribution range in the nature, low requirement on growth conditions, good environmental tolerance and high propagation speed, and the existing research shows that the growth of the chlorella in natural lake water in which water bloom occurs is far higher than that of microcystis aeruginosa, but the report is fresh for bacteria capable of dissolving the chlorella.
At present, most other patents can roughly divide the way of bacteria acting on microalgae into two types, one type is to apply algae-dissolving bacteria fermentation liquor or algae-dissolving bacteria fermentation supernatant to dissolve microalgae, for example, the patent with the patent number of 2015110052933 and the name of shortwave monospermum and application thereof is to directly apply bacteria cultured by beef extract peptone to dissolve algae, but because the nutrient components of the beef extract peptone are complex, the environment with algae-dissolving effect is not consistent with the water body environment with actual eutrophication, besides, the beef extract peptone culture medium also has certain influence on the growth of microalgae, and the algae-dissolving effect mode still has certain defects. The other is to extract the algicidal active substances secreted by the bacteria or to apply the algicidal bacteria to the algicidal after other materials are added to the algicidal bacteria to prepare microbial preparations. For example, patent No. 2014102454460 entitled "A. algicidal Chryseobacterium and its application in cyanobacteria bloom control" adopts algicidal active substance secreted by algicidal bacteria to solubilize algae, but its extraction procedure is complicated and time-consuming, and once water bloom is developed, it is difficult to control the algae propagation in effective time. For example, patent No. 2015103579966 entitled "composite microbial preparation and its application in treatment of water body with outbreak of algal bloom" uses algicidal bacteria to prepare microbial preparation for algae lysis, but because the activity of bacteria contained in microbial preparation changes with time, the algae control effect cannot be measured.
disclosure of Invention
Aiming at the defects in the technology, the invention aims to provide the application of the pseudomonas with the algae-dissolving activity, so as to solve the problems that the existing algae-dissolving bacteria application environment is not suitable for the water body environment in which eutrophication actually occurs and the problems of complexity and time consumption in extracting the algae-dissolving active substances.
In order to achieve the purpose, the invention provides the following scheme:
The invention provides application of Pseudomonas fragi X11 in removing microalgae in a water body.
the pseudomonas is X11, is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of CGMCC, China academy of sciences institute of China, No. 3, Siro 1, north Chen, south China), has the preservation number of CGMCC No.8398, and is proposed to be named as pseudomonas (Pseudomonas) in classification within 10 months and 18 days of 2013.
The pseudomonad strain Pseudomonas fragi X11 is cultured on LB agar plate medium at 15 deg.c for 48 hr to obtain colony of 2.5mm diameter. The bacterial colony is round and convex, has smooth surface, viscous texture, milk white color and translucency.
The Pseudomonas fragi X11 strain is characterized in that the bacterial cells are rod-shaped, and the cell size is 0.53 multiplied by 1-2.87 mu m.
The physiological and biochemical characteristics of the Pseudomonas fragi X11 strain are gram negative, the results of the catalase, oxidase, methyl red, gelatin liquefaction, hydrogen sulfide generation test and arginine double hydrolase reaction are positive, and the results of the indole production test, the starch hydrolysis test and the V-P test are negative.
The 16S rRNA gene sequence of the Pseudomonas fragi X11 strain is characterized in that the 16S rRNA gene sequence of the strain X11 has the length of 1419bp, and the accession number of KJ496054 in GeneBank. The homology of the strain X11 with Pseudomonas fragi ATCC4973 can reach 99.79 percent through the alignment with the GeneBank homologous sequence.
According to the morphological characteristics, physiological and biochemical characteristics of the strain, and the results of the alignment of the 16S rRNA gene sequence of the strain in GeneBank, the isolated strain X11 was identified as Pseudomonas sp, according to Bergey' S Manual of bacteriology for identification (8 th edition).
The microalgae is chlorella.
The method for removing the microalgae in the water body by the pseudomonas comprises the steps of carrying out activation culture on the pseudomonas in a shaking culture box with the temperature of 30 ℃ and the rotating speed of 120r/min for 14 hours, then carrying out transfer culture for 15 hours, centrifuging a zymophyte liquid, removing a supernatant, washing twice by using a phosphate buffer solution, resuspending zymophyte cells by using a chlorella culture medium, and then adding the cells into chlorella liquid for algae solubilization. The volume ratio of the chlorella liquid to the pseudomonas zymophyte liquid is 4: 1.
The invention discloses the following technical effects:
The Pseudomonas fragi X11 has strong inhibiting effect on growth and propagation of chlorella, the microalgae culture medium is used for resuspending algae-dissolving bacteria cells, the method is more suitable for water body environment with actual water body eutrophication, the algae-dissolving characteristic can be exerted on the water bloom algae in natural water body, and the method has wide application prospect in the aspect of water bloom treatment mainly based on chlorella outbreak. The invention uses zymocyte to dissolve algae, when the water bloom is outbreak, the use cost is lower, and the invention has the potential of large-scale use.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 shows chlorophyll contents at 0h and 120h after lysing chlorella at different bacterial cell concentrations;
FIG. 2 shows the algae lysis rate of chlorella for 120h with different bacterial cell concentrations;
FIG. 3 shows the number of chlorella cells after lysing the chlorella by bacterial cells;
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The algae used in the invention is chlorella (purchased from fresh water algae seed bank of institute of aquatic organisms of academy of sciences of China).
The invention uses a strain Pseudomonas fragi (Pseudomonas fragi) with the number of X11, and the strain is preserved in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms with the preservation number of CGMCC No. 8398.
Preparation of culture medium
The culture medium used for chlorella is NaNO 3 1500mg/L, K 2 HPO 4.3H 2 O40 mg/L, MgSO 4.7H 2 O75 mg/L, CaCl 2.2H 2 O36 mg/L, CaCl 2.2H 2 O, C 6 H 8 O 7 6mg/L, Fe (NH 4) 3 (C 6 H 5 O 7)6mg/L, Na 2 CO 3 20mg/L, EDTANa 2 1mg/L, C 6 H 12 O 6 10g/L, H 3 BO 3 2.86.86 mg/L, MnCl 2.4H 2 O1.81 mg/L, ZnSO 4.7H 2 O0.222 mg/L, NaMoO 4.2H 2 O0.39 mg/L, CuSO 4.5H 2 O0.079 mg/L, Co (NO 3) 2.6H 2 O0.0494 mg/L.
10g of peptone, 10g of yeast extract powder, 5g of NaCl and 1000ml of distilled water, wherein the pH value is approximately equal to 7, are used as an LB culture medium for Pseudomonas fragi X11.
Example 1 lytic Effect of Pseudomonas fragi X11 different cell concentrations on Chlorella
In order to study the algae-lysing effect of Pseudomonas fragrans fragi X11 on Chlorella vulgaris with different cell concentrations, the optimal cell concentration dosage is determined, the specific implementation steps are that 40ml of fresh Chlorella vulgaris treated in the example, the chlorophyll content is 5.68mg/L, OD 680 is 1.225, Pseudomonas pseudomonnas fragi X11 is inoculated into 100ml of sterilized LB culture medium, the culture medium is cultured for 14h in an incubator with 30 ℃ and 120rpm, 1ml of bacterial liquid is transferred into 100ml of sterilized LB liquid culture medium, the culture medium is cultured for 15h in an incubator with 30 ℃ and 120rpm, 20ml, 10ml and 4ml of fermented bacterial liquid are centrifuged, the supernatant is washed with phosphate buffer solution and discarded, the bacterial cells centrifuged in different volumes are added into 40ml of fresh Chlorella vulgaris when the bacterial liquid OD 600 is 1.2 ≈ 1.2, 20ml, 10ml and 4ml of fermented bacterial liquid is centrifuged, the supernatant is discarded, the volume ratio of Chlorella vulgaris liquid to fermentation liquid is 2:1 (treated group 1), 2: 1), 2 (treated group), 2: 1.539) and the control strains are added with good algae-lysing effect, the control strains are respectively, and the results are shown in the test chart that the Chlorella vulgaris 2: 1.11.11 mg/11.11 mg/11, 11 mg/11 mg of the treated bacterial liquid and the Chlorella strain are respectively.
The algicidal rate is calculated by the formula of (control chlorophyll concentration-treatment chlorophyll concentration)/control chlorophyll concentration × 100. Wherein the chlorophyll concentration is extracted with methanol.
example 2 Effect of Pseudomonas fragi X11 bacterial cells on Chlorella cell count
In order to examine the effect of the Pseudomonas fragi X11 cells on the number of chlorella cells, the cell concentration was selected at a chlorella cell volume ratio of 2:1, the chlorella cell count was 1.5 × 10 8 cells/mL, OD 680 was 1.225 for 40mL of fresh chlorella treated in this example, Pseudomonas fragi X11 was inoculated into 100mL of sterilized LB medium, cultured in an incubator at 30 ℃ and 120rpm for 14h, then the cell suspension was transferred into 100mL of sterilized LB liquid medium, cultured in an incubator at 30 ℃ and 120rpm for 15h, when the cell suspension OD counted to 1.2, 20mL of fermentation broth was centrifuged, the supernatant was washed twice with phosphate buffer, the cell suspension was centrifuged, the cell suspension was added to 40mL of fresh chlorella, that is a chlorella suspension to fermentation broth volume ratio of 2:1, the chlorella suspension without added bacterial cells was used as a control group, the cell count of chlorella suspension was taken under a microscope, and the results of the chlorella cell count were shown in a basic test showing that the number of chlorella cells was not affected by the chlorella cell count of a change test, and the chlorella strain X11 was not shown.
the above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (5)
1. Application of Pseudomonas fragi in removing microalgae in water body.
2. The use of claim 1, wherein the pseudomonas is numbered X11 and has been deposited at the china general microbiological culture collection center at 18 th 10 th 2013 with the deposit number of CGMCC No. 8398.
3. The use according to claim 1, wherein the microalgae is chlorella.
4. The application of claim 1, wherein the method for removing microalgae from water body by pseudomonas comprises the steps of performing activation culture on pseudomonas in a shaking culture box with the temperature of 30 ℃ and the rotation speed of 120r/min for 14h, performing transfer culture for 15h, centrifuging fermentation broth, discarding supernatant, washing twice by using phosphate buffer solution to obtain fermentation broth, resuspending the fermentation broth by using a chlorella culture medium, and adding the suspended fermentation broth into chlorella solution for algae solubilization.
5. The use of claim 4, wherein the ratio of the volume of chlorella liquid to the volume of pseudomonas zymophyte liquid is 4: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910900731.4A CN110550744A (en) | 2019-09-23 | 2019-09-23 | Application of pseudomonas with algae-lysing activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910900731.4A CN110550744A (en) | 2019-09-23 | 2019-09-23 | Application of pseudomonas with algae-lysing activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110550744A true CN110550744A (en) | 2019-12-10 |
Family
ID=68741190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910900731.4A Pending CN110550744A (en) | 2019-09-23 | 2019-09-23 | Application of pseudomonas with algae-lysing activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110550744A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471609A (en) * | 2019-12-26 | 2020-07-31 | 中国科学院水生生物研究所 | Pseudomonas with algae-lysing activity and application thereof |
| CN113583906A (en) * | 2021-07-21 | 2021-11-02 | 首都师范大学 | Application of pseudomonas B5 in algae removal |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050266036A1 (en) * | 2004-06-01 | 2005-12-01 | Agscitech | Microbial biosurfactants as agents for controlling pests |
| CN105907678A (en) * | 2016-05-10 | 2016-08-31 | 东北师范大学 | Low-temperature-resisting pseudomonas fragi and application thereof |
| CN109735472A (en) * | 2019-03-01 | 2019-05-10 | 中国科学院烟台海岸带研究所 | It is a kind of to produce electricity molten algae marine bacteria and its application |
-
2019
- 2019-09-23 CN CN201910900731.4A patent/CN110550744A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050266036A1 (en) * | 2004-06-01 | 2005-12-01 | Agscitech | Microbial biosurfactants as agents for controlling pests |
| EP1750738A1 (en) * | 2004-06-01 | 2007-02-14 | Salam M. Awada | Microbial biosurfactants as agents for controlling pests |
| CN105907678A (en) * | 2016-05-10 | 2016-08-31 | 东北师范大学 | Low-temperature-resisting pseudomonas fragi and application thereof |
| CN109735472A (en) * | 2019-03-01 | 2019-05-10 | 中国科学院烟台海岸带研究所 | It is a kind of to produce electricity molten algae marine bacteria and its application |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471609A (en) * | 2019-12-26 | 2020-07-31 | 中国科学院水生生物研究所 | Pseudomonas with algae-lysing activity and application thereof |
| CN111471609B (en) * | 2019-12-26 | 2022-03-15 | 中国科学院水生生物研究所 | Pseudomonas with algae-lysing activity and application thereof |
| CN113583906A (en) * | 2021-07-21 | 2021-11-02 | 首都师范大学 | Application of pseudomonas B5 in algae removal |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Khambata et al. | Studies on a new oxalate-decomposing bacterium, Pseudomonas oxalaticus | |
| CN109182215A (en) | One bacillus licheniformis bacterial strain and its application in inhibition bacterial pathogen | |
| CN106167776A (en) | A kind of can bacillus cereus (Bacillus cereus) TH 35 of heavy metal cadmium and application thereof in activating soil | |
| CN106754571B (en) | A kind of complex microorganism deodorant and preparation method thereof | |
| CN109762767B (en) | Sewage composite microbial treatment agent and preparation method and application thereof | |
| CN106754572A (en) | One bacillus amyloliquefaciens and application thereof | |
| CN110550744A (en) | Application of pseudomonas with algae-lysing activity | |
| CN113846039A (en) | Bacillus belgii and application thereof | |
| CN111662837B (en) | Bacillus atrophaeus and application thereof | |
| CN117070428B (en) | Application of bacillus subtilis BS-22 strain in improving cultivation environment | |
| CN112410261B (en) | Bacillus siamensis MC2-1 and application thereof | |
| CN112391323B (en) | Bacillus tequilensis D5-8 and application thereof | |
| CN109321500A (en) | One bacillus amyloliquefaciens bacterial strain and its application in prevention and treatment Oil Tea Anthracnose evil | |
| CN101914464B (en) | Alcaligenes sp. MB-N6 for removing nitrite nitrogen pollution out of water and application thereof | |
| CN105936879B (en) | Bacillus subtilis K13 and its cultural method and application | |
| Elmarzugi et al. | Production of α-amylase using new strain of Bacillus polymyxa isolated from sweet potato | |
| CN106434408A (en) | Citric acid bacillus Y3 with function of degrading brominated flame retardants and application thereof | |
| Kataoka et al. | Enrichment culture and isolation of slow-growing bacteria | |
| NL2029992B1 (en) | Nanocomposite for microbial remediation and preparation method and use thereof | |
| CN112574918B (en) | Ammonia nitrogen degrading bacteria, microbial agent and application thereof | |
| Prokšová et al. | Enrichment, isolation and characterization of dialkyl sulfosuccinate degrading bacteria Comamonas terrigena N3H and Comamonas terrigena N1C | |
| US20020064864A1 (en) | Microbial composition and a process useful for the neutralization of alkaline waste-waters | |
| CN109810918B (en) | Bacillus atrophaeus with effect of preventing wolfberry leaf blight, biological agent and application of biological agent | |
| CN102352326B (en) | Method of removing bloom-forming cyanobacteria by using Aeromonas sp. | |
| CN114437961B (en) | Bacillus amyloliquefaciens composite microbial agent for reducing water surface tension and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191210 |