CN110511066A - A method of Particles dispersed microbial bacterial agent is produced using span amino acid mother liquor - Google Patents
A method of Particles dispersed microbial bacterial agent is produced using span amino acid mother liquor Download PDFInfo
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- CN110511066A CN110511066A CN201910873986.6A CN201910873986A CN110511066A CN 110511066 A CN110511066 A CN 110511066A CN 201910873986 A CN201910873986 A CN 201910873986A CN 110511066 A CN110511066 A CN 110511066A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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Abstract
The embodiment of the invention discloses a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent, include the following steps: that step 100, separation biologic enzymolysis method prepare the span amino acid mother liquor after amino acid；Step 200 is diluted the span amino acid mother liquor after separation, and the physico chemical factor of span amino acid mother liquor is adjusted after dilution, and span amino acid mother liquor adjusted is divided into two parts, a portion is diluted to flowing shape again, carrier material after another part hybrid filtering, the Organic Ingredients carrier such as addition peanut shell powder are uniformly mixed；Step 300, Organic Ingredients carrier and microorganism fungus kind prepare mixing in proportion, and the span amino acid mother liquor after gradually spray dilutes in mixing is uniformly mixed compost fermentation；It is granulated after step 400, compost fermentation by pelletizer, is dry；The present invention substitutes granulation water and adhered soil by the by-product span amino acid mother liquor of biologic enzymolysis method amino acid institute output, increases material by 10%-12% Liquid amino acid content, improves nutrient, enhancing crop quality.
Description
Technical field
The present embodiments relate to fermentation technical fields, and in particular to a kind of micro- using span amino acid mother liquor production Particles dispersed
The method of bacteria agent.
Background technique
Microbial bacterial agent is very widely used, is widely used in planting industry, aquaculture even soil, water pollution and controls
In reason.With the popularization of its application range, the demand of yield is also increasing year by year.But conventional preparation method is often straight
The mode of biofermentation was connected to prepare, in this preparation method, the preparation of one side base fluid needs the time to prepare, and
And it initially due to not being zymogenic control environment needs that the rank normally fermented can be stepped by one section of repoductive time
Section,；Still further aspect is then fermentation and needs to be added a large amount of water and adhered soil during being granulated according to demand, and this portion
Divide substance and does not contain effective nutritional ingredient.
Summary of the invention
For this purpose, the embodiment of the present invention provides a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent,
To solve the problems, such as that fermentation efficiency can improve product entirety nutritional ingredient slowly and in granulation process in the prior art.
To achieve the goals above, embodiments of the present invention provide the following technical solutions:
A method of Particles dispersed microbial bacterial agent is produced using span amino acid mother liquor, which is characterized in that including walking as follows
It is rapid:
Step 100, separation biologic enzymolysis method prepare the span amino acid mother liquor after amino acid；
Step 200 is diluted the span amino acid mother liquor after separation, and adjusts the physical and chemical item of span amino acid mother liquor after dilution
Span amino acid mother liquor adjusted is divided into two parts by part, and a portion is diluted to flowing shape again, and another part mixed
Carrier material after filter is added organic raw material carrier and is uniformly mixed；
Step 300, Organic Ingredients carrier and microorganism fungus kind prepare mixing in proportion, after in mixing, gradually spray dilutes
Span amino acid mother liquor, be uniformly mixed compost fermentation；
It is granulated after step 400, compost fermentation by pelletizer, is dry.
As a preferred solution of the present invention, in step 100, the step of separation includes two steps:
Filtering, is filtered entire base fluid, and filtered carrier material is added organic raw material carrier and is crushed
Processing, keeps the humidity of carrier；
It extracts, filtered fluid is extracted, and acid-base neutralization is carried out to the extraction raffinate after extraction.
As a preferred solution of the present invention, in step 200, the extraction raffinate after separation is filtered again, and will
Filtered carrier material merges with the carrier material in step 100, is diluted to filtered span amino acid mother liquor, is diluting
The physico chemical factor for adjusting filtrate again afterwards forms span amino acid mother liquor.
As a preferred solution of the present invention, physico chemical factor includes temperature, pH value, miscellaneous bacteria quantity.
As a preferred solution of the present invention, in step 200, miscellaneous bacteria is carried out to the span amino acid mother liquor before dilution to go out
It is living, the specific steps of miscellaneous bacteria inactivation are as follows:
Organic acid is added into span amino acid mother liquor, and makes the pH value of solution below 4.5；
The temperature of solution is maintained, and 65 DEG C or more are heated to span amino acid mother liquor, gradually addition is few during addition
The temperature and fluctuation of concentration that the clear water of amount maintains solution maintain above-mentioned state 45~60 minutes in limited range；
Stop heating, and clear water is added thereto and is diluted and cools down.
As a preferred solution of the present invention, before carrying out Organic Ingredients carrier and microorganism fungus kind mixing, to micro-
Biological inoculum carries out selective cultivation:
Strain is transferred in culture medium, setting activation condition activated, after activation according to 1~3% inoculum concentration
Further logarithm is linked into amplification culture medium to increase；
High-temperature steam is carried out to culture medium before carrying out activation and logarithm growth and kills disinfection, and is suitable according to strain
The temperature and pH of suitable activation condition adjustment culture medium.
As a preferred solution of the present invention, in step 300, when Organic Ingredients carrier and microorganism fungus kind in proportion
Further span amino acid mother liquor is carried out when span amino acid mother liquor mass ratio after preparing mixed gross mass and diluting is greater than 6 dilute
It releases.
As a preferred solution of the present invention, the quality of carrier material and aqueous is adjusted according to production target requirement
Amount, method particularly includes:
When carrier material water content be greater than 40%, reduction moisture is carried out to carrier material, until water content be lower than 40%；
After carrying out precipitation point to carrier material, carried out according to the mass ratio 1:2 of carrier material it is uniformly mixed, it is equal in mixing
After even on the mixture by dry material shape covering, and the thickness of coating is not less than 2 centimetres；
The humidity range for maintaining coating bottom mixture is continuing fermentation between 25~40%.
As a preferred solution of the present invention, product is sufficiently humidified so as in the compost later period, and to production after compost
Object is squeezed and is filtered, and extracts filtered clear liquid, obtains complex micro organism fungicide after carrying out low temperature concentration to clear liquid.
As a preferred solution of the present invention, filtered residue is subjected to low temperature drying, and by the residue after drying
Evenly dispersed mix to Organic Ingredients carrier carries out low temperature drying and granulation.
Embodiments of the present invention have the advantages that
It is micro- in production particle using by-product span amino acid mother liquor after the present invention produces amino acid products by biologic enzymolysis method
During bacteria agent, granulation water is substituted, the granulation of span amino acid mother liquor spray shaping is all used instead, increases material by 10%-
While 12% Liquid amino acid, and bentonite needed for alternative granulating, adhered soil, cost is reduced, nutrient content is improved,
Root system development can be enhanced, the premise for synthesizing plant endogenous hormones polyamines improves the salt stress-resistant ability of crop.
Detailed description of the invention
It, below will be to embodiment party in order to illustrate more clearly of embodiments of the present invention or technical solution in the prior art
Formula or attached drawing needed to be used in the description of the prior art are briefly described.It should be evident that the accompanying drawings in the following description is only
It is merely exemplary, it for those of ordinary skill in the art, without creative efforts, can also basis
The attached drawing of offer, which is extended, obtains other implementation attached drawings.
Structure depicted in this specification, ratio, size etc., only to cooperate the revealed content of specification, for
Those skilled in the art understands and reads, and is not intended to limit the invention enforceable qualifications, therefore does not have technical
Essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size are not influencing the function of the invention that can be generated
Under effect and the purpose that can reach, should all still it fall in the range of disclosed technology contents obtain and can cover.
Fig. 1 is the flow diagram in embodiment of the present invention.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, person skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily, it is clear that described embodiment is the present invention one
Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not doing
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
As shown in Figure 1, the present invention provides a kind of methods using span amino acid mother liquor production complex micro organism fungicide, including
Following steps:
Step 100, separation biologic enzymolysis method prepare the by-product span amino acid mother liquor after amino acid；
Step 200 is diluted the span amino acid mother liquor after separation, and adjusts the physical and chemical item of span amino acid mother liquor after dilution
Part, and span amino acid mother liquor adjusted is divided into two parts, a portion is diluted to flowing shape, another part mixing again
Filtered carrier material, addition peanut shell powder are uniformly mixed；
Step 300, biological organic fertilizer bottom material and microorganism fungus kind prepare mixing in proportion, gradually spray dilution in mixing
Span amino acid mother liquor afterwards is uniformly mixed compost fermentation；
It is granulated after step 400, compost fermentation by pelletizer, is dry.
The main method for preparing complex micro organism fungicide in the present invention is still by conventional fermentation process system
It is standby, it is granulated after fermentation by spraying by-product aminoacids solution, thus the preparation of complete microbial inoculum.Based on above-mentioned preparation method,
It is added in the present embodiment and passes through pretreated span amino acid mother liquor, to reach the mesh for improving Liquid amino sour component in material
, it reduces the production cost of material and improves nutrient.
In step 100, the span amino acid mother liquor of biologic enzymolysis method by-product needs to mention after completing by enzymatic isolation method preparation
The target products such as amino acid are taken, generated span amino acid mother liquor is main addition in the present invention after being extracted target product
Object.
In step 100, the step of separation includes two steps:
The first step, filtering, is filtered entire base fluid, and filtered carrier material addition peanut shell powder is carried out powder
Broken processing, the humidity for keeping carrier material certain.
In conventional enzymatic isolation method, since enzymatic hydrolysis has specificity, inevitable be unable to get there is also many substances is filled
Point enzymatic hydrolysis, even and target protein will necessarily exist big also due to the factors such as environment can not be digested adequately
The carrier material of amount, and the hydrolysis of part can also occur in the environment of enzymatic hydrolysis for these carrier materials, the ingredient of hydrolysis is not
Target amino acid, but it is still main nutriment for plant, thus make use of momentum it is necessary used, with
The nutrient content of product is improved, and reduces production cost, reduces the generation of waste.
In order to enable carrier material can be mixed preferably with subsequent product, it is therefore desirable to be carried out at crushing to carrier material
Reason, and the further decomposition in order to promote carrier material, need to keep the humidity of carrier material, with lasting progress after filtration
Fermentation.
Second step extracts, and target amino acid is primarily present in filtered fluid, therefore is extracted to filtered fluid, by target
Separation for amino acids is gone out, and in order not to destroy the activity and trophic structure of surplus materials, carries out acid-base neutralization to the extraction raffinate after extraction.
In the present invention, supernatant fraction is added in material by way of spray, is substitution granulation water, all
It uses the granulation of span amino acid mother liquor spray shaping instead, therefore, is mainly realized by way of sprinkling in process, in order to avoid
Ponding to sprinkling, atomising device etc..In step 200, it needs to be filtered the raffinate after separation again, and incited somebody to action
Carrier material after filter merges with the carrier material in step 100, is diluted to filtered span amino acid mother liquor, after dilution
The physico chemical factor for adjusting filtrate again forms span amino acid mother liquor.
Among the above, physico chemical factor includes temperature, pH value, miscellaneous bacteria quantity.Wherein control temperature and pH value and be in order to
Promote fermentation and further continued hydrolysis, and controlling miscellaneous bacteria quantity is the consumption and drop for reducing miscellaneous bacteria to material effective component
The preservation of low harmful substance reduces the toxic action of miscellaneous bacteria existence.
In step 200, miscellaneous bacteria inactivation, the specific steps of miscellaneous bacteria inactivation are carried out to the span amino acid mother liquor before dilution are as follows:
Organic acid is added into span amino acid mother liquor, and makes the pH value of solution below 4.5；
The temperature of solution is maintained, and 65 DEG C or more are heated to span amino acid mother liquor, gradually addition is few during addition
The temperature and fluctuation of concentration that the clear water of amount maintains solution maintain above-mentioned state 45~60 minutes in limited range；
Stop heating, and clear water is added thereto and is diluted and cools down.
In the present embodiment, miscellaneous bacteria inactivation cannot be carried out by the way of conventional, this is because training in conventional method
Support base in without inoculation strain, can by directly by high temperature in a manner of inactivated, target strain is inoculated after inactivation.
However in the present invention, corresponding active material and strain are had existed in span amino acid mother liquor, according to conventional high temperature inactivation
Mode is handled, and will certainly destroy the activity of wherein unstable small-molecule active substance and strain, therefore by the temperature of solution
Degree is adjusted in low pH and higher temperature environment, and long-time high temperature is avoided to generate destruction to wherein active material.
In addition, after carrying out miscellaneous bacteria inactivation, before carrying out Organic Ingredients carrier and microorganism fungus kind mixing, to microorganism
Strain carries out selective cultivation:
Strain is transferred in culture medium, setting activation condition activated, after activation according to 1~3% inoculum concentration
Further logarithm is linked into amplification culture medium to increase；
High-temperature steam is carried out to culture medium before carrying out activation and logarithm growth and kills disinfection, and is suitable according to strain
The temperature and pH of suitable activation condition adjustment culture medium.
In the present embodiment, the control of strain is mainly passed through based on pH control, supplemented by the temperature of culture medium, thus
Guarantee the control of strain and the stabilization of active material configuration.
In step 200, according to the quantity of the quality of carrier material and water content selection peanut shell powder, specific method
Are as follows:
When the water content of carrier material is greater than 40%, carrier material is carried out reducing moisture until water content is lower than 40%；
After carrying out reduction moisture to carrier material, mixed according to the mass ratio 1:2 of carrier material and peanut shell powder
Uniformly, passing through dry powdered covering on the mixture after mixing, and the thickness of coating is not less than 2 centimetres；
The humidity range for maintaining coating bottom mixture is continuing fermentation between 25~40%.
In above-mentioned steps, the effect of moisturizing is to promote the fermentation of carrier material and keeps temperature, can keep load
Body material is in more preferably environment in spare period further to ferment, and discharges more nutriments, improves whole nutrient
Content.
In step 300, when Organic Ingredients carrier and microorganism fungus kind prepare mixed gross mass and dilution in proportion
Span amino acid mother liquor mass ratio afterwards is further diluted span amino acid mother liquor when being greater than 6.
In the present embodiment, it by multiple diluted mode, on the one hand improves to live in peace and calculates the total amount of mother liquor, another party
Face mainly promotes the active constituent in span amino acid mother liquor to be released by diluted mode, to improve living in mother liquor
Property substance ingredient, improve material in Liquid amino acid content.
In the present invention, other than carrying out fermentation based on span amino acid mother liquor and obtaining strain, also in the compost later period to product
It is sufficiently humidified so as to, and product is squeezed and filtered after compost, and extract filtered clear liquid, low temperature is carried out to clear liquid
Complex micro organism fungicide is obtained after concentration.
Complex micro organism fungicide is prepared in the present embodiment, and application mainly cooperates the corresponding material that is granulated to make
With, through the above way by microorganism therein carry out concentration extraction, be to adjust micro- life in material according to use demand
The content of object microbial inoculum.
Because being configured in the manner described above, contain the microbial bacterial agent of certain radix in material always, even if passing through
After the mode of concentration extraction obtains a part of microbial inoculum, the microbial inoculum of certain content is still had, it can in the subsequent application stage
The content of different phase microbial inoculum to be adjusted flexibly as desired.
In addition, filtered residue is carried out low temperature drying, and mix the residue after drying is evenly dispersed to organic original
Expect that carrier carries out low temperature drying and granulation.It is that high temperature is avoided to kill work to microbial inoculum therein generation by way of low temperature drying
With.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (10)
1. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent, which comprises the steps of:
Step 100, separation biologic enzymolysis method prepare the span amino acid mother liquor after amino acid；
Step 200 is diluted the span amino acid mother liquor after separation, and adjusts the physico chemical factor of span amino acid mother liquor after dilution,
Span amino acid mother liquor adjusted is divided into two parts, a portion is diluted to flowing shape, another part hybrid filtering again
Carrier material afterwards is added organic raw material carrier and is uniformly mixed；
Step 300, Organic Ingredients carrier and microorganism fungus kind prepare mixing in proportion, the ammonia after gradually spray dilutes in mixing
Base acid mother liquor is uniformly mixed compost fermentation；
It is granulated after step 400, compost fermentation by pelletizer, is dry.
2. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 1, special
Sign is, in step 100, the step of separation includes two steps: filtering is filtered entire base fluid, and will be after filtering
Carrier material add organic raw material carrier and carry out pulverization process, keep the humidity of carrier；
It extracts, filtered fluid is extracted, and acid-base neutralization is carried out to the extraction raffinate after extraction.
3. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 2, special
Sign is, in step 200, is filtered again to the extraction raffinate after separation, and will be in filtered carrier material and step 100
Carrier material merge, filtered span amino acid mother liquor is diluted, after dilution again adjust filtrate physico chemical factor shape
At span amino acid mother liquor.
4. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 3, special
Sign is that physico chemical factor includes temperature, pH value, miscellaneous bacteria quantity.
5. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 1, special
Sign is, in step 200, carries out miscellaneous bacteria inactivation, the specific steps of miscellaneous bacteria inactivation to the span amino acid mother liquor before dilution are as follows:
Organic acid is added into span amino acid mother liquor, and makes the pH value of solution below 4.5；
The temperature of solution is maintained, and 65 DEG C or more are heated to span amino acid mother liquor, is gradually added on a small quantity during addition
The temperature and fluctuation of concentration that clear water maintains solution maintain above-mentioned state 45~60 minutes in limited range；
Stop heating, and clear water is added thereto and is diluted and cools down.
6. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 1, special
Sign is, before carrying out Organic Ingredients carrier and microorganism fungus kind mixing, carries out selective cultivation to microorganism fungus kind:
Strain is transferred in culture medium, setting activation condition is activated, and is accessed after activation according to 1~3% inoculum concentration
Into amplification culture medium, further logarithm increases；
High-temperature steam is carried out to culture medium before carrying out activation and logarithm growth and kills disinfection, and is suitable for according to strain
The temperature and pH of activation condition adjustment culture medium.
7. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 1, special
Sign is, in step 300, after Organic Ingredients carrier and microorganism fungus kind prepare mixed gross mass and dilution in proportion
Span amino acid mother liquor mass ratio be greater than 6 when further span amino acid mother liquor is diluted.
8. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 2, special
Sign is, the quality and water content of carrier material are adjusted according to production target requirement, method particularly includes:
When carrier material water content be greater than 40%, reduction moisture is carried out to carrier material, until water content be lower than 40%；
After carrying out precipitation point to carrier material, carried out according to the mass ratio 1:2 of carrier material it is uniformly mixed, after mixing
On the mixture by dry material shape covering, and the thickness of coating is not less than 2 centimetres；
The humidity range for maintaining coating bottom mixture is continuing fermentation between 25~40%.
9. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 1, special
Sign is, is sufficiently humidified so as in the compost later period to product, and product is squeezed and filtered after compost, and extracts filtering
Clear liquid afterwards obtains complex micro organism fungicide after carrying out low temperature concentration to clear liquid.
10. a kind of method using span amino acid mother liquor production Particles dispersed microbial bacterial agent according to claim 9, special
Sign is, filtered residue is carried out low temperature drying, and mixes the residue after drying is evenly dispersed to Organic Ingredients carrier
Carry out low temperature drying and granulation.
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Cited By (1)
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Application publication date: 20191129 |