CN110510758A - A method of utilizing lead ion in Mycobacterium graminis removal sewage - Google Patents
A method of utilizing lead ion in Mycobacterium graminis removal sewage Download PDFInfo
- Publication number
- CN110510758A CN110510758A CN201910853038.6A CN201910853038A CN110510758A CN 110510758 A CN110510758 A CN 110510758A CN 201910853038 A CN201910853038 A CN 201910853038A CN 110510758 A CN110510758 A CN 110510758A
- Authority
- CN
- China
- Prior art keywords
- lead ion
- flotation
- microbial adsorbent
- culture medium
- sewage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Water Treatment By Sorption (AREA)
Abstract
The invention discloses a kind of methods using lead ion in Mycobacterium graminis removal sewage, strain is accessed in agar solid slope culture medium or agar solid plate culture medium after nitrogenous fluid nutrient medium carries out continuous four generations Liquid Culture activation and is saved, the strain is linked into nitrogenous culture medium from the agar solid slope culture medium or agar solid plate culture medium, at 28-30 DEG C, shaken cultivation 5d under the conditions of 120r/min, up to microbial adsorbent, by centrifugation or microbial adsorbent wet cell is obtained by filtration in microbial adsorbent, microbial adsorbent wet cell, which is added in flotation cell in the sewage containing lead ion, carries out absorption flotation recovery separation, finally recycle microbial adsorbent and lead ion;The microbial adsorbent of this method preparation has good Adsorption effect for the lead ion in sewage, and at low cost, is easy to industrial applications.
Description
Technical field
The present invention relates to the fields of heavy metal ions in sewage processing, especially a kind of to handle sewage using Mycobacterium graminis
The method of middle lead ion.
Background technique
In the prior art, heavy metal ion contained in sewage is difficult to be biodegradable after entering environment, because its have compared with
High toxicity, into water body after can be migrated with water, finally accumulated in vivo by food chain, destroy organism normal physiological
Metabolic activity, and the ecological environment on periphery and the life quality of the mankind are seriously threatened, so the processing to effluent containing heavy metal ions
And its recycling is current one of important subject.Biotechnology as a kind of environmental protection, sewage treatment efficiently, economic and
Recycling new technology has been increasingly subject to the concern of people.
Sewage composition is complicated, involve a wide range of knowledge, influence factor is more, distinct, need to administer refer mainly to indicate hardenability,
Total hardness, three nitrogen, heavy metal ion, chloride, sulfate, fluoride, pH value etc., wherein endanger maximum factor first is that weight
Metal ion is a Xiang Chongdian in one of current focus concerned by people and environmental pollution improvement to effectively removing for it
Content.
The processing method of heavy metal wastewater thereby can be divided into two major classes.The first kind makes the heavy metal ion in waste water in solubilised state
It is changed into insoluble heavy metal compound, is removed from waste water through precipitating and floatation.Specific method have neutralisation, vulcanization,
Reduction method, oxidizing process, ion-exchange, ion floatation method, activated carbon method, ferrite process, electrolysis method and diaphragm electrolysis etc..Second
Heavy metal in waste water is concentrated and is separated under conditions of not changing its chemical form by class, and specific method has reverse osmosis
Method, electro-osmosis method, evaporation concentration method etc..Although these methods can be realized to the adsorption cleaning of certain heavy metal ion, generally deposit
Heavy metal ion type, limited amount can be being adsorbed, adsorbent cannot be reached and the shortcomings that heavy metal recycles.
In recent years, using biomaterial, heavy-metal ion removal has carried out more and more researchs from weak solution, also takes
Many important progress were obtained, recycling heavy metal are removed using biotechnology, in the side such as investment, operation, operational administrative and technique
Face is also superior to traditional administering method, these characteristics make environmental microorganism wastewater processing technology towards it is nontoxic, harmless, without secondary
The direction of pollution steps again and goes a step further.
Summary of the invention
In view of the problems of the existing technology, sewage is removed using Mycobacterium graminis the purpose of the present invention is to provide a kind of
The method of middle lead ion, by by it is a kind of through culture, be enriched with microbial adsorbent, micro collecting agent is added after in heavy metal
It is stirred absorption in waste water solution, uses method for floating separating and recycling microbe adsorbent after the completion of absorption, removes lead ion, and
Obtain the industrial sewage process method of aqueous solution up to standard.
To achieve the above object, technical scheme is as follows:
A method of using lead ion in Mycobacterium graminis removal sewage, step includes:
One, strain accessed after nitrogenous fluid nutrient medium carries out the activation of continuous four generations Liquid Culture agar solid slope culture medium or
It is saved in agar solid plate culture medium, by the strain from the agar solid slope culture medium or agar solid plate culture
It is linked into base in nitrogenous culture medium, shaken cultivation 5d is under the conditions of 28-30 DEG C, 120r/min to get microbial adsorbent;
Two, the microbial adsorbent passes through centrifugation or filtering, obtains the microbial adsorbent adsorbed for lead ion
Wet cell;
Three, the microbial adsorbent wet cell is added in flotation cell in the sewage containing lead ion, by being thoroughly mixed,
Reach adsorption equilibrium after absorption 10min;
Four, flotation recovery separation is carried out to saturation absorption microbial adsorbent in the flotation cell, in speed of agitator 100-
Under conditions of 200r/min, pH=4.0 ~ 7.0, collecting agent is added, flotation recovery is saturated microorganism absorbent 8min, takes described floating
Tail washings in slot is selected, is drained after detection is qualified;
Five, the bacteria residue for being collected into flotation recovery collects, and desorption agent solution is added, and stirs desorption with the speed of 100r/min
10min, centrifuge separation, is separately recovered microbial adsorbent and lead ion.
Further, in the first step, the culture medium dissolved with agar is poured at 1/3 in test tube, inclined-plane is set,
Or formation plate at 1/2 in culture dish is poured into, in aseptic operating platform after ultraviolet lamp sterilizing 30min, by the careless branch bar
Bacterium is seeded on the agar surface culture medium or the agar solid plate culture medium, at 28-30 DEG C, 160r/min
Under conditions of, for 24 hours, taking-up is put into biochemical cultivation case shaken cultivation, under the conditions of 4 DEG C of deepfreeze, is saved stand-by.
Further, the concentration of lead ion is 60-200mg/L in the sewage.
Further, alkaline earth metal cation solution, the alkaline earth metal cation solution packet are added in Xiang Suoshu flotation cell
Include NH4Cl、NaCl、MgCl2, KCl solution.
Further, the temperature of flotation is 24-28 DEG C of room temperature in the four steps, and flotation mixing speed is 160r/min.
Further, in the first step, the formula and component of the nitrogenous fluid nutrient medium and the nitrogenous culture medium
It is all the same.
Further, in the second step, the microbial adsorbent supernatant is outwelled after centrifugation or
Semi-permeable membrane filtering can obtain the microbial adsorbent wet cell.
Further, in the four steps, collecting agent is lauryl amine, the CAS accession number of the flotation collector lauryl amine
For 1502-03-0, chemical formula C12H25N。
Further, the strippant is sodium carbonate, and the CAS accession number of the sodium carbonate is 497-19-8, and chemical formula is
Na2CO3。
Further, in the 5th step, desorbing the microbial adsorbent after the recovery can be by flotation again, or warp
Centrifugation is separated with strippant, and reproducible utilization 4 times.
A kind of method using lead ion in Mycobacterium graminis removal sewage in the present invention, Mycobacterium graminis,
Mycobacterium belongs to bacterium domain, Firmicutes, mycobacteriaceae, Mycobacterium on taxology
(Mycobacterium).Mycobacterium graminis is widely present in nature, especially universally present in soil and plant leaf, institute
To be sometimes referred to as it as bacillus subtills or timothy grass bacillus.It is harmless, all not pathogenic to all animals.Mycobacterium graminis is applied to
The removal of heavy metal ions in wastewater is that flotation performance is superior, and Mycobacterium graminis surface protein content is more since it is adsorbed,
Make it have preferable hydrophobicity, test shows that its contact angle is 75 °, Mycobacterium graminis under common physiological condition, by
In its surface carboxyl groups and phosphate anion functional group presence and there is negative surface charge, the isoelectric point in pure water is 3.0
;Under the influence of different p H values and the external environments such as the exposure of ionic strength metal ion and surfactant, due to its surface
The ionization state of functional group changes, and makes to be easier to bonding, cohesion between cell, promotes cell and heavy metal ion
Absorption and flotation effect.The cell of Mycobacterium graminis as gram-positive bacteria and the lipid content of cell wall are high, and contain
The mycolic acids component of 60 ~ 80 carbon atoms.In addition, Mycobacterium graminis cell wall has 4 layers of structure: peptidoglycan layer, rouge from the inside to surface
Albumin layer, outer membrane and teichoic acid layer, peptidoglycan layer contain meso-diaminopimelic acid, arabinose and galactolipin and minority
The macromolecular compound reticular structure that the small peptide of amino acid composition is polymerized;Lipoprotein layer is by lipid part and protein portion
It constitutes, it is connected between peptide glycan and outer membrane, is integrally formed the two;Outer membrane is typical phophoslipid bilayer structure, in phosphatide
Nonpolar base attract each other and arrange inwardly, polar group is mutually exclusive to be arranged outwardly;Lipoteichoicacid is located on outer membrane, is in cell
Wall outermost layer,My.pThe performance of bacterium is mainly determined by it.Lipoteichoicacid is by 3 lipoid A, core polysaccharide and O- specific side chain portions
It is grouped as, but a large amount of ingredient is lipoid A.Each lipoid A is made of 2 N-Acetyl-D-glucosamines and 5 long chain fatty acids,
Therefore, lipopolysaccharides layer not only contains a large amount of nonpolar hydrocarbon chain, also containing a large amount of polar carboxylic acid groups.
There are mainly two types of the modes of action of microorganism and heavy metal ion: one is change heavy metal by biological effect
Chemical form reduces its mobility and bioavailability in the environment, heavy metal is made to fix or detoxify;Second is that utilizing bacterium
The suction-operated of object and heavy-metal ion removal.According to biological adsorption metal whether the metabolism of dependent cells, biological adsorption machine
Reason is divided into metabolism dependent form and non-metabolism dependent form;Again according to the positioning of the metal removed from solution, biological adsorption is divided into:
(1) extracellular aggregation or precipitating;(2) cell surface absorption or precipitating;(3) aggregation intracellular.
The Mycobacterium graminis microbial adsorbent of the application preparation has good Adsorption effect to heavy metal ion;
And microbial adsorbent culture of the invention is simply at low cost, adsorption effect is good, is easy to industrial applications, realizes metal ion
Recycle, widened the concentration range of biological wastewater treatment heavy metal ion, protected environment, realize heavy metal ion and
Utilization is separately recovered in water, has good Social benefit and economic benefit.
Detailed description of the invention
Fig. 1 is adsorption test flow chart of the invention;
Fig. 2 is that microbial adsorbent prepared by the present invention desorbs absorption-flotation of the frequency to Mycobacterium graminis to heavy metal ion
Removal effect figure.
Specific embodiment
In order to make those skilled in the art more fully understand the solution of the present invention, below with reference to attached in example of the present invention
Technical scheme in the embodiment of the invention is clearly and completely described for figure, it is clear that described example is only the present invention
A part of example, rather than whole example.Based on middle example of the invention, those skilled in the art are not being done
Out under the premise of creative work, every other embodiment obtained be should fall within the scope of the present invention.
In the description of present embodiment, the orientation of the instructions such as term "inner", "outside", "front", "rear", "left", "right" or
Positional relationship is to be based on the orientation or positional relationship shown in the drawings, it is only for convenient for the description present invention and simplify description,
Rather than the device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation, because
This is not considered as limiting the invention.In addition, term " first ", " second " are only used for distinguishing similar object, and cannot
It is interpreted as specific sequence or precedence, it should be appreciated that such use is wanted can be interchanged in appropriate situation.
To clearly demonstrate design philosophy of the invention, below with reference to example, the present invention will be described.
A kind of method using lead ion in Mycobacterium graminis removal sewage of the present invention, step include:
One, strain accessed after nitrogenous fluid nutrient medium carries out the activation of continuous four generations Liquid Culture agar solid slope culture medium or
It is saved in agar solid plate culture medium, by the strain from the agar solid slope culture medium or agar solid plate culture
It is linked into base in nitrogenous culture medium, shaken cultivation 5d is under the conditions of 28-30 DEG C, 120r/min to get microbial adsorbent;
Two, the microbial adsorbent passes through centrifugation or filtering, obtains the microbial adsorbent adsorbed for lead ion
Wet cell;
Three, the microbial adsorbent wet cell is added in flotation cell in the sewage containing lead ion, by being thoroughly mixed,
Reach adsorption equilibrium after absorption 10min;
Four, flotation recovery separation is carried out to saturation absorption microbial adsorbent in the flotation cell, in speed of agitator 100-
Under conditions of 200r/min, pH=4.0 ~ 7.0, collecting agent is added, flotation recovery is saturated microorganism absorbent 8min, takes described floating
Tail washings in slot is selected, is drained after detection is qualified;
Five, the bacteria residue for being collected into flotation recovery collects, and desorption agent solution is added, and stirs desorption with the speed of 100r/min
10min, centrifuge separation, is separately recovered microbial adsorbent and lead ion.
In order to which what is be more clear is illustrated the technical solution in the present invention, carried out in the form of specific embodiment below
Explanation.
A kind of method using Mycobacterium graminis removal heavy metal ions in sewage of the present invention, step include:
One, strain accessed after nitrogenous fluid nutrient medium carries out the activation of continuous four generations Liquid Culture agar solid slope culture medium or
It is saved in person's agar solid plate culture medium, by the strain from the agar solid slope culture medium or agar solid plate
It is linked into culture medium in nitrogenous culture medium, shaken cultivation 5d is under the conditions of 28-30 DEG C, 120r/min to get microorganism adsorption
Agent;
Two, the microbial adsorbent outwells the clear liquid on upper layer after centrifugation, obtains adsorbing for lead ion micro-
Biological adsorption agent wet cell;
Three, the microbial adsorbent wet cell is added in flotation cell in the sewage containing lead ion, by being thoroughly mixed,
Reach adsorption equilibrium after absorption 10min;
Four, flotation recovery separation is carried out to saturation absorption microbial adsorbent in the flotation cell, in speed of agitator 100-
Under conditions of 200r/min, pH=4.0 ~ 7.0, collecting agent lauryl amine is added, flotation recovery is saturated microorganism absorbent 8min, takes
Tail washings in the flotation cell is drained after detection is qualified;
Five, the bacteria residue for being collected into flotation recovery collects, and desorption agent solution is added, and stirs desorption with the speed of 100r/min
10min, centrifuge separation, is separately recovered microbial adsorbent and lead ion.
In above-mentioned example, the Mycobacterium graminis microbial adsorbent of the application preparation there is good absorption to go lead ion
Except effect;And microbial adsorbent culture of the invention is simply at low cost, adsorption effect is good, is easy to industrial applications, realizes
The recycling of lead ion has widened the concentration range of biological wastewater treatment lead ion, has protected environment, realize lead ion and
Utilization is separately recovered in water, has good Social benefit and economic benefit.
In above-mentioned steps one, nitrogenous Liquid Culture based formulas are as follows: beef extract 3g, peptone 5g, sodium chloride 5g, water
1000mL, pH7.0~7.2.When preparing nitrogenous fluid nutrient medium, culture medium prescription first is accurately weighed with electronic balance (0.1g)
Each composition material composition, successively puts it into the conical flask for fill distilled water and dissolves by heating, portable high pressure is put into after encapsulation
In Sterilization Kettle, 120 DEG C at a temperature of sterilize 30min, it is cooling after pendulum on cleaning sterile station with ultraviolet light irradiation
30min, then long-term dry powder freezing strain is seeded in aforesaid liquid culture medium and carries out continuous culture activation.
In above-mentioned steps one by Mycobacterium graminis from agar solid slope culture medium or agar solid plate culture medium
It is linked into nitrogenous culture medium, then will there is bacterium culture medium to be put into HZQ-C type constant temperature air bath oscillation case, at 28 DEG C, 160r/
After cultivating 5d under conditions of min, it is stand-by as microbial adsorbent to take out bacterium solution.
The preparation method of two kinds of Solid agar culture is roughly the same with nitrogenous fluid nutrient medium in step 1, area
It is not the agar powder of the addition 2% when nitrogenous fluid nutrient medium dissolves, then heating fusing sterilizes together.It will dissolution when use
There is the culture medium of agar to pour at 1/3 in test tube, set inclined-plane, or pour into formation plate at 1/2 in culture dish, sterile
In station after ultraviolet lamp sterilizing 30min, the Mycobacterium graminis is seeded to the agar surface culture medium or institute
It states on agar solid plate culture medium, under conditions of 28-30 DEG C, 160r/min, for 24 hours, taking-up is put into biochemical training to shaken cultivation
It supports in case, under the conditions of 4 DEG C of deepfreeze, saves stand-by.It the use of Solid agar culture is Mycobacterium graminis in order to prevent
Activation breeding is failed after mostly generation in nitrogenous fluid nutrient medium, can effectively be slowed down after strain is seeded in Solid agar culture
The division growth of strain.
In above-mentioned steps two, step 3, after microbial adsorbent is outwelled the clear liquid on upper layer after centrifugation
To microbial adsorbent wet cell, microbial adsorbent wet cell is put into the sewage in flotation cell, specific implementation is for example
Under:
Embodiment one
Absorbing process of the microbial adsorbent Mycobacterium graminis to copper ions in waste water: obtained microbial adsorbent is wet thin
Born of the same parents are added in the flotation cell containing 60-200mg/L lead ion aqueous solution with the injected volume of 9.0g/L, and the pH of sewage is 4.0 ~
7.0, it is then micro- to saturation absorption in flotation cell by flotation cell stirring and adsorbing 10min after putting into microbial adsorbent wet cell
Biological adsorption agent carries out flotation recovery separation, under conditions of speed of agitator 100-200r/min, pH=4.0 ~ 7.0, to careless branch
The lauryl amine flotation 8min of 0.06mg/L is added in bacillus bacteria suspension, the bacteria residue of flotation recovery is put into the sodium carbonate of 200mg/L
Desorption and regeneration in solution, microbial adsorbent regeneration is using after 4 times, and lead ion content is lower than 40.0mg/L in water, in flotation cell
Bacteria suspension concentration be down to 0.01mg/L or less.Tail washings in the flotation cell is taken, is drained after detection is qualified.
The collecting agent lauryl amine cation that flotation is adsorbed in the present invention is mainly wet thin with electrostatic interaction and microbial adsorbent
Born of the same parents' Mycobacterium graminis adsorbs, meanwhile, the complexing generated between the copper ion that the cell surface of microbial adsorbent is adsorbed
Effect also plays certain activation to the absorption flotation recovery of collecting agent lauryl amine.As shown in Figure 1, for the micro- life of the present invention
Object adsorbent Mycobacterium graminis and collecting agent lauryl amine handle the process flow containing heavy metal polluted waste water.
The bacteria residue that finally flotation recovery is collected into collects, and desorption agent solution is added, is stirred with the speed of 100r/min
Desorption 10min is mixed, microbial adsorbent wet cell and lead ion is separately recovered in centrifuge separation.
In above-mentioned example, as shown in Fig. 2, applicant passes through a large number of experiments, the experimental results showed that, add with into flotation cell
The increase of the microbial adsorbent Mycobacterium graminis content added, the adsorption rate of lead ion are also in increased trend, when microorganism inhales
When the dosage of attached dose of wet cell reaches 6.0mg/L, lead ion eliminating rate of absorption starts to increase rapidly, after reaching 8.5g/L, increases
Long amplitude slows down, and after microbial adsorbent wet cell dosage reaches 9.0g/L, lead ion eliminating rate of absorption has reached maximum value,
So the injected volume selected in the present embodiment is 9.0g/L.
Embodiment two
In flotation cell be added alkali metal cation solution for microbial adsorbent Mycobacterium graminis in waste water contain lead ion
Adsorption effect influence: obtained microbial adsorbent wet cell is added to the injected volume of 9.0g/L containing 60-
In the flotation cell of 200mg/L lead ion aqueous solution, the pH of sewage is 4.0 ~ 7.0, after putting into microbial adsorbent wet cell, is added
The NH of 5.3mg/L4Cl solution, by flotation cell stirring and adsorbing 10min, lead ion content is lower than 20.0mg/L in water after absorption, so
Afterwards in flotation cell to saturation absorption microbial adsorbent carry out flotation recovery separation, speed of agitator 100-200r/min, pH=
Under conditions of 4.0 ~ 7.0, the collecting agent lauryl amine of 0.06mg/L is added into Mycobacterium graminis bacteria suspension, flotation recovery saturation is micro-
After bio-absorbable agent 8min, the bacteria suspension concentration in flotation cell is down to 0.01mg/L or less.Tail washings in the flotation cell is taken, through examining
Drain after surveying qualification.
The collecting agent lauryl amine cation that flotation is adsorbed in the present invention is mainly wet thin with electrostatic interaction and microbial adsorbent
Born of the same parents' Mycobacterium graminis adsorbs, meanwhile, the complexing generated between the zinc ion that the cell surface of microbial adsorbent is adsorbed
Effect also plays certain activation to the absorption flotation recovery of collecting agent lauryl amine.As shown in Figure 1, for the micro- life of the present invention
Object adsorbent Mycobacterium graminis and collecting agent lauryl amine handle the process flow containing heavy metal polluted waste water.
The bacteria residue that finally flotation recovery is obtained collects, and sodium carbonate is added and desorbs agent solution, with the speed of 100r/min
Microbial adsorbent wet cell and lead ion is separately recovered in degree stirring desorption 10min, centrifuge separation.
In above-mentioned example, be added alkali metal cation solution chlorination ammonium salt solution after, Mycobacterium graminis for Pb In Exhausted Water from
The flotation effect of son has been significantly improved, while as shown in Fig. 2, grass divides after multiple parsing-absorption-flotation
The absorption efficiency of branch bacillus is still very high.
Embodiment three
Obtained microbial adsorbent wet cell is added to the injected volume of 9.0g/L water-soluble containing 60-200mg/L lead ion
In the flotation cell of liquid, the pH of sewage is 4.0 ~ 7.0, and after putting into microbial adsorbent wet cell, NaCl solution 60.0mg/L is added
And MgCl2Solution 95.0mg/L, by flotation cell stirring and adsorbing 10min, lead ion content is lower than 20.0mg/L in water after absorption, so
Afterwards in flotation cell to saturation absorption microbial adsorbent carry out flotation recovery separation, speed of agitator 100-200r/min, pH=
Under conditions of 4.0 ~ 7.0, the collecting agent lauryl amine of 0.06mg/L, flotation recovery saturation are added into the bacteria suspension of Mycobacterium graminis
After microorganism absorbent 8min, the bacteria suspension concentration in flotation cell is down to 0.01mg/L or less.Tail washings in the flotation cell is taken, is passed through
Drain after detection is qualified.
The collecting agent lauryl amine cation that flotation is adsorbed in the present invention is mainly wet thin with electrostatic interaction and microbial adsorbent
Born of the same parents' Mycobacterium graminis adsorbs, meanwhile, the complexing generated between the lead ion that the cell surface of microbial adsorbent is adsorbed
Effect also plays certain activation to the absorption flotation recovery of collecting agent lauryl amine.As shown in Figure 1, for the micro- life of the present invention
Object adsorbent Mycobacterium graminis and collecting agent lauryl amine handle the process flow containing heavy metal polluted waste water.
The bacteria residue that finally flotation recovery is collected into collects, and sodium carbonate is added and desorbs agent solution, with 100r/min's
Microbial adsorbent wet cell and lead ion is separately recovered in speed stirring desorption 10min, centrifuge separation.
Example IV
Obtained microbial adsorbent wet cell is added to the injected volume of 9.0g/L water-soluble containing 60-200mg/L lead ion
In the flotation cell of liquid, the pH of sewage is 4.0 ~ 7.0, after putting into microbial adsorbent wet cell, addition KCl solution 75mg/L,
NaCl solution 60.0mg/L and NH4Cl solution 95.0mg/L, by flotation cell stirring and adsorbing 10min, lead ion contains in water after absorption
Amount is lower than 20.0mg/L, then carries out flotation recovery separation to saturation absorption microbial adsorbent in flotation cell, turns in stirring
Under conditions of fast 100-200r/min, pH=4.0 ~ 7.0, the collecting agent of 0.06mg/L is added into the bacteria suspension of Mycobacterium graminis
Lauryl amine, after flotation recovery is saturated microorganism absorbent 8min, the bacteria suspension concentration in flotation cell is down to 0.01mg/L or less.It takes
Tail washings in the flotation cell is drained after detection is qualified.
The collecting agent lauryl amine cation that flotation is adsorbed in the present invention is mainly wet thin with electrostatic interaction and microbial adsorbent
Born of the same parents' Mycobacterium graminis adsorbs, meanwhile, the complexing generated between the lead ion that the cell surface of microbial adsorbent is adsorbed
Effect also plays certain activation to the absorption flotation recovery of collecting agent lauryl amine.As shown in Figure 1, for the micro- life of the present invention
Object adsorbent Mycobacterium graminis and collecting agent lauryl amine handle the process flow containing heavy metal polluted waste water.
The bacteria residue that finally flotation recovery is collected into collects, and sodium carbonate is added and desorbs agent solution, with 100r/min's
Microbial adsorbent wet cell and lead ion is separately recovered in speed stirring desorption 10min, centrifuge separation.
The problem of can not rationally utilizing the present invention overcomes resource in the prior art, exists respectively in above-mentioned several embodiments
Different alkali metal cation solution is added in flotation cell, compared to the feelings that alkali metal cation solution is added not in flotation cell
Condition, alkali metal cation solution obviously can promote Mycobacterium graminis for the assimilation effect of Pb In Exhausted Water ion;And such as Fig. 2
Shown, after alkali metal cation solution is added, Mycobacterium graminis after duplicate desorption, imitate by absorption-flotation of cell
Fruit is still very high.
For the sewage for containing different heavy metal species ions, realize adsorbent and heavy metal ion is recyclable sharp again
With, the processing cost of sewage is saved, and treatment effeciency is high, simple process, without secondary pollution, in entire operation process,
Experimental condition is not harsh, suitable for being promoted in entire industry.
It should be noted that some of which structure can have different selections other than the above-mentioned specific example provided.And
These are all that those skilled in the art can make on the basis of understanding inventive concept based on its basic skills, therefore herein
No longer enumerate.
Finally, it will be appreciated that the principle that embodiment of above is intended to be merely illustrative of the present and the example that uses
Property embodiment, however the present invention is not limited thereto.For those of ordinary skills, do not depart from it is of the invention
In the case where principle and essence, various changes and modifications can be made therein, these variations and modifications are also considered as protection model of the invention
It encloses.
Claims (10)
1. a kind of method using lead ion in Mycobacterium graminis removal sewage, which is characterized in that step includes:
One, strain accessed after nitrogenous fluid nutrient medium carries out the activation of continuous four generations Liquid Culture agar solid slope culture medium or
It is saved in agar solid plate culture medium, by the strain from the agar solid slope culture medium or agar solid plate culture
It is linked into base in nitrogenous culture medium, shaken cultivation 5d is under the conditions of 28-30 DEG C, 120r/min to get microbial adsorbent;
Two, the microbial adsorbent passes through centrifugation or filtering, obtains the microbial adsorbent adsorbed for lead ion
Wet cell;
Three, the microbial adsorbent wet cell is added in flotation cell in the sewage containing lead ion, by being thoroughly mixed,
Reach adsorption equilibrium after absorption 10min;
Four, flotation recovery separation is carried out to saturation absorption microbial adsorbent in the flotation cell, in speed of agitator 100-
Under conditions of 200r/min, pH=4.0 ~ 7.0, collecting agent is added, flotation recovery is saturated microorganism absorbent 8min, takes described floating
Tail washings in slot is selected, is drained after detection is qualified;
Five, the bacteria residue for being collected into flotation recovery collects, and desorption agent solution is added, and stirs desorption with the speed of 100r/min
10min, centrifuge separation, is separately recovered microbial adsorbent and lead ion.
2. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that described
In first step, the culture medium dissolved with agar is poured at 1/3 in test tube, sets inclined-plane, or pour into 1/2 in culture dish
Place forms plate, and in aseptic operating platform after ultraviolet lamp sterilizing 30min, the Mycobacterium graminis is seeded to the agar solid
On plating medium or the agar solid plate culture medium, under conditions of 28-30 DEG C, 160r/min, shaken cultivation
For 24 hours, it takes out and is put into biochemical cultivation case, under the conditions of 4 DEG C of deepfreeze, save stand-by.
3. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that described
The concentration of lead ion is 60-200mg/L in sewage.
4. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that institute
Addition alkaline earth metal cation solution in flotation cell is stated, the alkaline earth metal cation solution includes NH4Cl、NaCl、MgCl2、
KCl solution.
5. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that described
The temperature of flotation is 24-28 DEG C of room temperature in four steps, and flotation mixing speed is 160r/min.
6. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that described
In first step, the formula and component of the nitrogenous fluid nutrient medium and the nitrogenous culture medium are all the same.
7. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that described
In second step, the microbial adsorbent after centrifugation by supernatant outwell or semi-permeable membrane filtering can obtain it is described micro-
Biological adsorption agent wet cell.
8. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that described
In four steps, collecting agent is lauryl amine, and the CAS accession number of the flotation collector lauryl amine is 1502-03-0, chemical formula
For C12H25N。
9. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that described
Strippant is sodium carbonate, and the CAS accession number of the sodium carbonate is 497-19-8, chemical formula Na2CO3。
10. the method according to claim 1 using lead ion in Mycobacterium graminis removal sewage, which is characterized in that institute
It states in the 5th step, desorbing the microbial adsorbent after the recovery can be by flotation again, or through centrifugation, with strippant
Separation, reproducible utilization 4 times.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910853038.6A CN110510758A (en) | 2019-09-10 | 2019-09-10 | A method of utilizing lead ion in Mycobacterium graminis removal sewage |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910853038.6A CN110510758A (en) | 2019-09-10 | 2019-09-10 | A method of utilizing lead ion in Mycobacterium graminis removal sewage |
Publications (1)
Publication Number | Publication Date |
---|---|
CN110510758A true CN110510758A (en) | 2019-11-29 |
Family
ID=68632020
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910853038.6A Pending CN110510758A (en) | 2019-09-10 | 2019-09-10 | A method of utilizing lead ion in Mycobacterium graminis removal sewage |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110510758A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111693687A (en) * | 2020-05-28 | 2020-09-22 | 长沙都正生物科技有限责任公司 | Preparation method of biological negative sample and biological standard substance and TMAO detection method |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2191752C2 (en) * | 1999-09-27 | 2002-10-27 | Государственный научный центр прикладной микробиологии | Biological preparation for removing crude oil and petroleum products from ground |
CN1379084A (en) * | 2002-05-20 | 2002-11-13 | 山东大学 | Process for deeply removing organosulfur from fossil fuel by mycobacteria |
CN102911904A (en) * | 2012-11-01 | 2013-02-06 | 北京科技大学 | Method for quickly screening microbial collecting agent for flotation of refractory hematite ore |
KR101341822B1 (en) * | 2013-10-11 | 2013-12-16 | 주식회사 티에스케이그린바이로 | Aerobic dechlorination system and process thereof |
CN104326625A (en) * | 2014-10-29 | 2015-02-04 | 太原理工大学 | Treatment method of slime water |
WO2018106791A1 (en) * | 2016-12-06 | 2018-06-14 | University Of South Carolina | Amelioration of oil toxicity and stimulation of bacterial oil degradation |
US20180194654A1 (en) * | 2013-03-15 | 2018-07-12 | Janet Angel | Composition and Methods of Use |
CN109499548A (en) * | 2018-12-25 | 2019-03-22 | 国家***天津海水淡化与综合利用研究所 | A kind of preparation method of biological adsorption agent and method using biological adsorption agent processing Lead In Sea Water ion |
-
2019
- 2019-09-10 CN CN201910853038.6A patent/CN110510758A/en active Pending
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2191752C2 (en) * | 1999-09-27 | 2002-10-27 | Государственный научный центр прикладной микробиологии | Biological preparation for removing crude oil and petroleum products from ground |
CN1379084A (en) * | 2002-05-20 | 2002-11-13 | 山东大学 | Process for deeply removing organosulfur from fossil fuel by mycobacteria |
CN102911904A (en) * | 2012-11-01 | 2013-02-06 | 北京科技大学 | Method for quickly screening microbial collecting agent for flotation of refractory hematite ore |
US20180194654A1 (en) * | 2013-03-15 | 2018-07-12 | Janet Angel | Composition and Methods of Use |
KR101341822B1 (en) * | 2013-10-11 | 2013-12-16 | 주식회사 티에스케이그린바이로 | Aerobic dechlorination system and process thereof |
CN104326625A (en) * | 2014-10-29 | 2015-02-04 | 太原理工大学 | Treatment method of slime water |
WO2018106791A1 (en) * | 2016-12-06 | 2018-06-14 | University Of South Carolina | Amelioration of oil toxicity and stimulation of bacterial oil degradation |
CN109499548A (en) * | 2018-12-25 | 2019-03-22 | 国家***天津海水淡化与综合利用研究所 | A kind of preparation method of biological adsorption agent and method using biological adsorption agent processing Lead In Sea Water ion |
Non-Patent Citations (2)
Title |
---|
周东琴等: "沟戈登氏菌对重金属的生物吸附-浮选和解吸性能", 《环境科学》 * |
魏德洲等: "草分枝杆菌吸附-浮选重金属的表面化学特征", 《中国环境科学学会2006年学术年会优秀论文集(中卷)》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111693687A (en) * | 2020-05-28 | 2020-09-22 | 长沙都正生物科技有限责任公司 | Preparation method of biological negative sample and biological standard substance and TMAO detection method |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104014314B (en) | Bio-adsorbent, preparation method and application | |
CN108359663A (en) | A kind of polyP bacteria immobilized spherule and its application | |
CN102277742A (en) | Preparation method of cleaning material of heavy metal ions in reusable water | |
CN106701732A (en) | Preparation method and application of micrococcus luteus immobilized beads | |
CN103436519A (en) | Composite microbial preparation for watershed bioremediation as well as preparation method and application thereof | |
CN101348306B (en) | Process for degrading cultivation waste water nitrite using immobilized nitrifying bacteria | |
CN110510758A (en) | A method of utilizing lead ion in Mycobacterium graminis removal sewage | |
Habeeb et al. | Removal of nickel (II) ions, low-level pollutants, and total bacterial colony count from wastewater by composite nanofiber film | |
CN102114409B (en) | Novel silver loaded activated carbon and preparation process thereof | |
Yu et al. | Anti-biological contamination strategies for enhanced uranium extraction from seawater | |
CN110526421A (en) | A method of heavy metal ions in sewage is removed using Gordonia bronchialis is hooked | |
CN109603750B (en) | Containing Fe3O4Preparation method of-GQDs composite gel adsorption film | |
CN105920773B (en) | A kind of microbial inoculum and preparation method for triazine herbicide of degrading | |
CN110510751A (en) | A kind of quick cultivation bacteria agent applied to domestic sewage processing system | |
CN101816920B (en) | Modified phanerochaete chrysosporium adsorbent as well as preparation and application thereof | |
CN108504695A (en) | A kind of extracellular polymeric and preparation method thereof, heavy metal treatment agent and process for treating heavy-metal waste water | |
Bin et al. | Study on the flocculability of metal ions by Bacillus mucilaginosus GY03 strain | |
CN102994394B (en) | Fungal strain LP-18-3 and application of fungal strain LP-18-3 in lead-containing water body treatment | |
CN101974448A (en) | Acinetobacter and application thereof to biological treatment of heavy metal ions | |
CN105154375B (en) | One kind is for the degrade remaining microbial inoculum of phenoxy carboxylic acid herbicides and preparation method | |
CN102294227B (en) | Preparation method and application method of chlorella seawater purifying biological adsorbent | |
CN106244502A (en) | One strain efficient dephosphorization and the pseudomonas of degraded lecithin | |
CN106419388A (en) | Portable [Sihua] (activating, purifying, benefiting and energizing) water cup | |
CN116656552A (en) | Application of pseudo-intermediate brucella in preparation of microbial inoculum for efficiently adsorbing and converting heavy metals | |
CN106145383A (en) | A kind of solid union microbial bacterial agent administered for black and odorous water |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191129 |