CN102911904A - Method for quickly screening microbial collecting agent for flotation of refractory hematite ore - Google Patents
Method for quickly screening microbial collecting agent for flotation of refractory hematite ore Download PDFInfo
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- CN102911904A CN102911904A CN2012104319712A CN201210431971A CN102911904A CN 102911904 A CN102911904 A CN 102911904A CN 2012104319712 A CN2012104319712 A CN 2012104319712A CN 201210431971 A CN201210431971 A CN 201210431971A CN 102911904 A CN102911904 A CN 102911904A
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Abstract
The invention discloses a method for quickly screening a microbial collecting agent for flotation of refractory hematite ore, wherein many microorganisms are obtained through selective culture of a bacteria culture medium, the microorganisms which are not suitable for being used as a hematite ore collecting agent in soil are quickly eliminated through preliminary screening of a flotation method, and a few of microbial varieties which are probability used as the hematite ore collecting agent are obtained. The microorganisms which are obtained through preliminary screening of the flotation are subjected to amplification culture and plate separation by using the bacteria culture medium to obtain a single strain; then a high-efficiency hematite ore microbial collecting agent which has higher yield and is non-pathogenic to the hematite ore is quickly screened through secondary screening of the flotation method; and the microorganism has a hydrophobic flocculation-flotation effect on the hematite ore under the condition that the using amount is 1/3 less than that of the conventional hematite ore collecting agent during flotation of the hematite ore. By using the microbial collecting agent screened by the flotation method, a large amount of microorganisms which are not suitable for being used as the hematite ore collecting agent in soil can be eliminated through preliminary screening of flotation, and the microorganism which has higher yield and is non-pathogenic to the hematite ore can be obtained through secondary screening of flotation.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of from soil method---the flotation process of microorganism collecting agent of screening refractory hematite, this method can be fast filters out the microorganism collecting agent of the suitable flotation refractory hematite of a strain in the numerous microorganism from the soil of iron ore stope in conjunction with bacteria culture medium.
Background technology
China's hematite reserves are abundant, but main " difficult ore dressing " form low with grade, that disseminated grain size is thin and combining form is complicated exists.The characteristics of this " poor, thin, assorted ", so that out the hematite overwhelming majority of exploitation needs fine grinding to arrive-200 orders 80%～90%, indivedual regional ores need fine grinding above 90% to-320 orders, iron mineral wherein could obtain the monomer cleavage and sorting.At present, the most effectual way that fine grinding like this " difficult ore dressing " is processed is flotation, but the flotation effect of Conventional flotation is very poor, and increasing the mineral floating particle diameter by flocculating polymer or hydrophobic flocculation is the important method that improves flotation effect.The most successful industrial application of flocculating polymer-flotation of rhombohedral iron ore surely belongs to U.S. Tilden and selects factory, this factory adopts starch to make Hematite flocculate into macrobead, remove siliceous sludge by desliming again, the cation-collecting agent reverse flotation is removed coarse grained siliceous gangue and is obtained iron ore concentrate.But the mechanical entrapment that selective flocculation has, to ore pulp ion sensitive, need the weakness such as utmost point thin liquid pulp sorting to limit its practical application.Hydrophobic flocculation mainly is by adding surfactant-based material such as collecting agent etc., making the purpose component form first again floatation recovery of hydrophobic floc sedimentation.As with the flocculation-collecting agent of lauryl amine acetate as rhombohedral iron ore, can make the rate of recovery of Hematite improve 20～30% than Conventional flotation.Hydrophobic flocculation-flotation has higher selectivity, thereby shows good application prospect.But the conventional chemical collecting agent is high to the poor selectivity of Hematite, medicine consumption, price and the companion's and the medicament environmental pollution problem that comes is still very big drawback.Therefore, the rhombohedral iron ore collecting agent of research efficient pollution-free or less contamination is to solve an urgent demand that China's lean hematite utilizes problem, and the research and development of microorganism collecting agent then is likely an important directions that solves an above-mentioned difficult problem.
Many microorganisms that nature exists, its surface is with in various degree electronegative and wettability, similar performance with conventional surfactants class collecting agent can equally with collecting agent be adsorbed on mineral surface, and with itself character adjust or change mineral surface electrically and hydrophobicity.In a single day the microorganism of surface hydrophobicity is adsorbed on the fine-particle minerals surface, not only can cause the hydrophobization on fine-particle minerals surface, also can make hydrophobic mineral grain attract each other into Hydrophobic aggregation.That propose the earliest the research of microorganism collecting agent is the Smith R W of U.S. Reynolds university, the microorganism collecting agent that obtains the earliest studying is Mycobacterium phlei (Mycobacterium phlei), and this microorganism can make the Hematite particle form hydrophobic floc sedimentation and floatation recovery.Along with the report of Smith R W article, the various countries researchist also begins actively to seek various performances and is similar to the microorganism of M.phle for the flotation of rhombohedral iron ore and other mineral.At present, the microorganism that can be used as the rhombohedral iron ore collecting agent of having studied also comprises: Bacillus polymyxa, Paenibacillus polymyxa, Rhodococcus opacus, Bacillus subtilis.These microorganisms are the same with M.phle, have equally to make the Hematite particle form the effect of hydrophobic floc sedimentation-flotation.
At present, the applied research of microorganism collecting agent is more and more, but the high-effective microorganism collecting agent how to screen for certain mineral floating from environment does not appear in the newspapers so far yet.Make a general survey of the screening method of existing microorganism, without unexceptional all are characteristic and the physiological functions that rely on microorganism itself, adopt substratum selection cultivation or dull and stereotyped isolation by dilution method.The screening of the microorganism collecting agent of hematite flotation also must take these methods as the basis, be screened according to the characteristic whether microorganism has a collecting agent without doubt again.In floatation process, as long as the microorganism that can be adsorbed in the rhombohedral iron ore surface and cause the rhombohedral iron ore surface hydrophobicity, can use as the collecting agent of rhombohedral iron ore, but only have those high adsorption capacities, the microorganism that is enough to make the rhombohedral iron ore surface hydrophobicity and form hydrophobic floc sedimentation and no pathogenicity could use as the high-efficient collecting agent of Hematite.
Summary of the invention
The present invention adopts first the flotation process primary dcreening operation to eliminate fast and is not suitable as the microorganism that the rhombohedral iron ore collecting agent uses in the soil, sieve again with flotation process again and sift out fast the efficient rhombohedral iron ore microorganism collecting agent that rhombohedral iron ore is had higher recovery and unlikely characteristic of disease, and this microorganism is hydrophobic floc sedimentation-flotation to the flotation of rhombohedral iron ore.
The present invention utilizes bacteria culture medium to be combined with flotation process, behind the non-bacterial micro-organism, directly adopts the flotation process primary dcreening operation to eliminate fast and is not suitable as the bacterial micro-organism that the rhombohedral iron ore collecting agent uses in the soil in the superseded soil with bacteria culture medium.The bacterial micro-organism that the flotation primary dcreening operation is obtained adopts bacteria culture medium amplification culture, plate isolation to obtain single thalline microorganism again.Adopt at last flotation process to sieve again to sift out fast 1 strain has higher recovery and unlikely characteristic of disease to rhombohedral iron ore efficient rhombohedral iron ore microorganism collecting agent, and this microorganism is hydrophobic floc sedimentation-flotation to the flotation of rhombohedral iron ore, and consumption is 1/3 of conventional chemical collector dosage.
Screening step, the condition of this microorganism collecting agent are: at first adopt bacteria culture medium that the soil pattern of fetching is carried out bacterium and select to cultivate.Take by weighing the soil pattern 10g through removal of impurities, add and to fill in the triangular flask of 90ml sterilized water, place 30 ℃, the shaking table of the 200r/min 20min that vibrates, leave standstill, obtain supernatant liquor.Inoculum size by 5% is inoculated in supernatant liquor in the bacterial liquid substratum, and shaking table is cultivated 24h, presses same method switching twice again, and centrifugal, washing obtains soil and mixes bacterium.Preserve soil and mix bacterium in the glycerine pipe, remaining bacterium liquid places 4 ℃ of refrigerators for subsequent use.
Soil mixes bacterium to be eliminated by the flotation primary dcreening operation rhombohedral iron ore not to be had adsorption or adsorption arranged but the bacterium that is not enough to cause the rhombohedral iron ore surface hydrophobicity, and the recycling bacteria culture medium carries out that bacterial liquid is cultivated, the dull and stereotyped cultivation, obtains single thalline.Again each single thalline is carried out flotation at last and sieve again, obtain a strain has higher recovery to hematite flotation microorganism collecting agent.Adopt the methods such as 16SrDNA complete sequence classification evaluation, SEM, infrared spectra, Zeta potential, contact angle determination that this strain microorganism collecting agent of determining is carried out multianalysis, obtain this strain microorganism basic data relevant with flotation.
Advantage of the present invention
On the basis that utilizes the bacteria culture medium screening, flotation has the microorganism collecting agent of similar chemical collecting agent effect to refractory hematite to adopt the flotation process rapid screening, and this microorganism collector dosage is chemical collector dosage 1/3, the rhombohedral iron ore particle is had the effect of adsorption-flocculation-flotation, so be particularly suitable as the collecting agent use of Hematite.
Utilize flotation process screening microorganism collecting agent of the present invention, only need just can eliminate through the flotation primary dcreening operation being not suitable as the microorganism that the rhombohedral iron ore collecting agent uses in a large amount of soil, the process flotation is sieved again and can be obtained a strain to hematite flotation rate of recovery height non-pathogenic microorganism again again.
Description of drawings
Accompanying drawing 1 is the process of rapid screening flotation refractory hematite microorganism collecting agent from the iron ore Stope Soil.
Embodiment
For describing better the present invention, flotation process screening microorganism collecting agent of the present invention is described in further detail with embodiment below in conjunction with accompanying drawing.
Embodiment 1
The flotation primary dcreening operation: the soil that obtains by the bacteria culture medium selective screening mixes in the bacterium, and the microorganism that is suitable as the use of rhombohedral iron ore collecting agent enters the come-up product with rhombohedral iron ore in floatation process, and the microorganism in the flotation cell is the microorganism for eliminating then.Used soil is taken near the Si Jiaying iron ore stope, Hebei, is the soil apart from ground 5～15cm place of diagonal method collection.Flotation test carries out in the small-sized flotation cell of 25 mL, adopts one section flotation flowsheet.Manually scrape bubble, the flotation water is distilled water, and aeration quantity is 2600 r/min.Take by weighing the pure rhombohedral iron ore of 2g iron content 68.11%, add in the flotation cell with deionized water and stir 2min, size mixing to setting the pH value with 1M HCl or 1M NaOH, add again microorganism and stir 10min, scrape bubble 5min.Through the flotation primary dcreening operation, eliminated being not suitable as the microorganism that the rhombohedral iron ore collecting agent uses, greatly reduced the microbe species that flotation is sieved again.
Flotation is sieved again: the microorganism that the flotation primary dcreening operation obtains is in process bacteria culture medium amplification culture, and plate isolation obtains multiple single strain, but which kind of is suitable as the flotation collector use of rhombohedral iron ore most in the single strain, adopts the method for hematite flotation to carry out multiple sieve again.Sieve again by flotation, that strain microorganism of and no pathogenicity maximum with the rate of recovery is used as the collecting agent of hematite flotation, and the evaluation performance relevant with flotation is with the explanation Flotation Mechanism.Flotation test carries out in the small-sized flotation cell of 25 mL, adopts one section flotation flowsheet.Manually scrape bubble, the flotation water is distilled water, and aeration quantity is 2600 r/min.Take by weighing the pure rhombohedral iron ore of 2g iron content 68.11%, add in the flotation cell with deionized water and stir 2min, size mixing to setting the pH value with 1M HCl or 1M NaOH, add again the single thalline of microorganism and stir 10min, scrape bubble 5min.The larger microorganism of the rate of recovery is carried out the classification of 16SrDNA complete sequence identify the ownership of determining microorganism, and firm this strain microorganism performance relevant with flotation.
Claims (1)
1. the method for the microorganism collecting agent of a rapid screening flotation refractory hematite, it is characterized in that: adopt first the flotation process primary dcreening operation to eliminate fast and be not suitable as the microorganism that the rhombohedral iron ore collecting agent uses in the soil, sieve again with flotation process again and sift out fast the efficient rhombohedral iron ore microorganism collecting agent that rhombohedral iron ore is had higher recovery and unlikely characteristic of disease, and this microorganism is hydrophobic floc sedimentation-flotation to the flotation of rhombohedral iron ore.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109321498A (en) * | 2018-10-16 | 2019-02-12 | 苏州市农业科学院 | A kind of screening technique of resistance to huge sum of money microorganism belonging to genus |
| CN110510758A (en) * | 2019-09-10 | 2019-11-29 | 中交铁道设计研究总院有限公司 | A method of utilizing lead ion in Mycobacterium graminis removal sewage |
| CN110773331A (en) * | 2019-09-30 | 2020-02-11 | 河南大学 | Enrichment device for separating micro-plastics in environmental soil and sediments |
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| CN102489415A (en) * | 2011-12-06 | 2012-06-13 | 北京科技大学 | Application of rhodococcuserythropolis in separation of hematite and separation method thereof |
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| CN102489415A (en) * | 2011-12-06 | 2012-06-13 | 北京科技大学 | Application of rhodococcuserythropolis in separation of hematite and separation method thereof |
Non-Patent Citations (3)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109321498A (en) * | 2018-10-16 | 2019-02-12 | 苏州市农业科学院 | A kind of screening technique of resistance to huge sum of money microorganism belonging to genus |
| CN109321498B (en) * | 2018-10-16 | 2021-09-10 | 苏州市农业科学院 | Screening method of heavy metal-resistant microorganisms |
| CN110510758A (en) * | 2019-09-10 | 2019-11-29 | 中交铁道设计研究总院有限公司 | A method of utilizing lead ion in Mycobacterium graminis removal sewage |
| CN110773331A (en) * | 2019-09-30 | 2020-02-11 | 河南大学 | Enrichment device for separating micro-plastics in environmental soil and sediments |
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Application publication date: 20130206 |