CN110477381A - A kind of albumin amino acid Calcium oral liquid prescription and preparation method with immunological regulation healthcare function - Google Patents
A kind of albumin amino acid Calcium oral liquid prescription and preparation method with immunological regulation healthcare function Download PDFInfo
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- CN110477381A CN110477381A CN201910825886.6A CN201910825886A CN110477381A CN 110477381 A CN110477381 A CN 110477381A CN 201910825886 A CN201910825886 A CN 201910825886A CN 110477381 A CN110477381 A CN 110477381A
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- 239000011575 calcium Substances 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 239000007788 liquid Substances 0.000 title claims abstract description 30
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 22
- 102000009027 Albumins Human genes 0.000 title claims abstract description 17
- 108010088751 Albumins Proteins 0.000 title claims abstract description 17
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 title claims abstract description 17
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 17
- 230000001900 immune effect Effects 0.000 title claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 42
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 24
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 21
- 150000001875 compounds Chemical class 0.000 claims abstract description 18
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 18
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 18
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 15
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 15
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims abstract description 14
- 235000014103 egg white Nutrition 0.000 claims abstract description 14
- 210000000969 egg white Anatomy 0.000 claims abstract description 14
- 229940100688 oral solution Drugs 0.000 claims abstract description 14
- 230000007062 hydrolysis Effects 0.000 claims abstract description 10
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 238000006243 chemical reaction Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- 239000004365 Protease Substances 0.000 claims description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 11
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- 239000012141 concentrate Substances 0.000 claims description 10
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- 238000001914 filtration Methods 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 9
- 241000382353 Pupa Species 0.000 claims description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 9
- 235000019419 proteases Nutrition 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 8
- 235000014655 lactic acid Nutrition 0.000 claims description 8
- 108090000526 Papain Proteins 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- 239000003513 alkali Substances 0.000 claims description 7
- 235000019834 papain Nutrition 0.000 claims description 7
- 229940055729 papain Drugs 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- 229920000858 Cyclodextrin Polymers 0.000 claims description 6
- 239000001116 FEMA 4028 Substances 0.000 claims description 6
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 6
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 6
- 229960004853 betadex Drugs 0.000 claims description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims description 6
- 206010013786 Dry skin Diseases 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 5
- 239000006071 cream Substances 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000002525 ultrasonication Methods 0.000 claims description 5
- 108090000145 Bacillolysin Proteins 0.000 claims description 2
- 102000035092 Neutral proteases Human genes 0.000 claims description 2
- 108091005507 Neutral proteases Proteins 0.000 claims description 2
- 239000000654 additive Substances 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims description 2
- 238000004821 distillation Methods 0.000 claims description 2
- 230000036039 immunity Effects 0.000 abstract description 7
- 238000011049 filling Methods 0.000 abstract description 6
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- 238000005238 degreasing Methods 0.000 abstract 1
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- 230000006870 function Effects 0.000 description 15
- 238000012360 testing method Methods 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 3
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 3
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- 239000000126 substance Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
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- 108010013534 Auxilins Proteins 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000931705 Cicada Species 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102100023922 Putative tyrosine-protein phosphatase auxilin Human genes 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
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- 230000036772 blood pressure Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- -1 compound amino acid Chemical class 0.000 description 1
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- 230000007812 deficiency Effects 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 230000004957 immunoregulator effect Effects 0.000 description 1
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- 210000000822 natural killer cell Anatomy 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
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- 229940023492 oral liquid product Drugs 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
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- 150000003254 radicals Chemical class 0.000 description 1
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- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Immunology (AREA)
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- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
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- Marine Sciences & Fisheries (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
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Abstract
The formula and preparation method of the present invention provides a kind of albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function, belong to food processing technology field.Oral solution provided by the invention is made using egg-white powder, degreasing silkworm chrysalis, pearl as raw material through the techniques such as enzymatic hydrolysis, concentration, allotment, homogeneous, constant volume, filling, sterilizing；Compound protein peptide is prepared using double-enzyme hydrolysis, water-soluble pearl calcium powder is prepared using acid-enzyme hydrolysis, while being aided with ultrasound, microwave treatment, improves protein hydrolysis degree and reaction efficiency；Effective component absorption of human body utilization rate in oral solution prepared by this patent is high, and strengthen immunity functional effect is obvious, and dissolubility is good, and flavour is palatable, and quality is high.
Description
Technical field
The present invention relates to a kind of albumin amino acid Calcium oral liquid prescription and system with immunological regulation healthcare function
Preparation Method belongs to food processing technology field.
Background technique
Egg white is intradermal semifluid colloidal substance under eggshell, contains 9%~11% protein in egg white, albumen has
A variety of amino acid of needed by human body are a kind of ideal and good protein resources.China processes finished product based on egg-white powder, but
Due to its large viscosity, fishy smell weight, heated easily solidification, dissolubility difference etc., application of the egg-white powder in food processing is by very
Big limitation.Research shows that the polypeptide anion in egg can cut down human free radical, reducing human aging enhances human body
Immunity can be absorbed by way of oligopeptides, and this substance can not only keep the original nutritive value of protein, additionally it is possible to
Alleviate the Competition that free amino acid is generated due to absorbing site jointly and further promotes being absorbed and utilized for protein.
Silkworm chrysalis is just applied as nourishing and fit keeping function and taste, the food of long muscle and drug from ancient times, and modern study is also demonstrate,proved
Its real nutritional ingredient rich in.Pupa albumen is ideal natural nutrition nitrogen source, the natural compound ammonia extracted from silkworm chrysalis
Base acid contains 18 kinds of amino acid required for growth in humans's development, metabolism, and component is comprehensive, and proportion is reasonable.Hydrolyze silkworm chrysalis
Profile of essential amino acids is obtained close to the compound amino acid liquid of human milk mode, is the new food protein resource with Development volue.
Pearl is rare medicinal material in China's successive dynasties medicine ancient books, have heart tonifying, strong bone, improving eyesight, relieving convulsion, blood pressure lowering,
Tranquilize the mind and promote the intelligence, strong body lengthen one's life, skin maintenance and cosmetic benefits, clearing heat and detoxicating and other effects.Some researches show that pearl powders to have certain increasing in recent years
The effect of strong immune function.Through biotechnology, treated that water-soluble pearl calcium contains a large amount of ionic calcium carbonate and a variety of machines
The substances such as element, small-molecular peptides, the amino acid that body needs, most of nutritional ingredient can be directly absorbed by the body, be chiefly used in
Orally taken for curing health care boosts metabolism by adjusting human endocrine and enhances immunity of organisms, prevent cardiovascular disease,
Improve cardiac function and inhibit cancer cell hyperplasia etc..
Summary of the invention
It is an object of the invention to solve the deficiencies in the prior art, provide that one kind can long-term safety be edible, nutritive value
High, the albumin amino acid Calcium oral liquid prescription with strengthen immunity function and preparation method.
In order to achieve the above-mentioned object of the invention, the invention provides the following technical scheme:
A kind of formula of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function, every 1000 milliliters of oral solutions
It include: 300~360g of egg-white powder, 140~200g of degreased pupa powder, 20~30g of pearl powder, citric acid 5g, sucrose 100g.
Preferably, a kind of albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function is recommended daily
Dose is 50mL/ people/day.
A kind of preparation method of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function, feature exist
In the following steps are included:
(1) preparation of compound protein peptide: egg-white powder, degreased pupa powder are dissolved in 10 times of amount distilled water, power 120W is used
Ultrasonication 10 minutes, papain is first added and digests 3~4h under the conditions of 60 DEG C, with 0.5mol/L NaOH solution tune
Whole enzymatic hydrolysis pH value of solution adds alkali protease and digests 4~5h, boiling water bath enzyme deactivation 10min under the conditions of 60 DEG C to 8.0~8.5,
Enzymolysis liquid sock filtration, filtered fluid are added appropriate beta-cyclodextrin and stir 30min, are then centrifuged 20min with 8000r/min revolving speed,
It takes 60 DEG C of supernatant to be concentrated in vacuo to the 1/8~1/10 of original volume, obtains compound protein peptide concentrate, it is spare；
(2) preparation of water-soluble pearl calcium powder: pearl is clean, drying, crushing sieve with 100 mesh sieve, and weigh pearl powder according to the ratio and add
Enter 2.5 times of amount lactic acid and be aided with 250W ultrasonic treatment 20min while stirring in water-bath, then boils 2~3h of hydrolysis, constantly stir
It mixes, filters after reaction, filtrate is individually stored；Filter residue adds appropriate amount of deionized water, with Ca (OH)2 PH value is adjusted to 7.0, is added
Enter 0 .3g neutral protease enzymolysis, enzyme deactivation 10min is boiled after enzymatic hydrolysis, enzymolysis liquid filtering merges acidolysis filtrate and enzymatic hydrolysis filter
Liquid mixes, and 50 DEG C of vacuum concentrations are at dry cream, and 40 DEG C of low temperature dryings, crush to get water soluble pearl powder in an oven；
(3) water-soluble pearl calcium powder prepared by citric acid, sucrose and step (2) preparation of oral solution: is dissolved in a small amount of distillation
In water, the compound protein peptide concentrate that step (1) preparation is then added is stirred evenly, and using 15MPa pressure homogeneous, constant volume is filled
Dress is vented (70~80 DEG C, 10 min), sealing, and 100 DEG C of 10~15min of sterilizing are in water-bath to get oral solution finished product.
Preferably, protease additive amount described in the step (1) is respectively 8000U/g papain, 10000U/g
Alkali protease.
Preferably, filter residue enzymatic hydrolysis condition after the hydrolysis of lactic acid described in the step (2) are as follows: microwave power 400W, temperature 50
DEG C, enzymatic hydrolysis 5~6h.
Compared with prior art, a kind of albumin amino acid pearl with immunological regulation healthcare function of the present invention
Ca oral liquid has the advantages that (1) using ultrasonic treatment and double-enzyme hydrolysis egg white, cicada pupa albumen, prepares compound protein
Peptide improves protein hydrolysis degree and dissolubility, promotes absorption of human body to utilize, while reducing sensitization；(2) it is adsorbed using beta-cyclodextrin
The peculiar smell of protein hydrolyzate is dispelled in processing, improves product mouthfeel and flavor；(3) using ultrasonic wave added lactic acid hydrolyzed pearl powder, micro-
Wave auxilin enzyme enzymatic hydrolysis prepares water-soluble pearl calcium and improves reaction rate, and preparation process flow is short, at low cost, effective component is damaged
It loses few；(4) product prepares compound protein peptide with egg white, silkworm chrysalis, prepares oral solution in conjunction with water-soluble pearl calcium, protein is converted
Promote absorption of human body for small-molecular peptides and amino acid, give full play to immunoregulatory healthcare function, develops that dissolubility is good, quality
High, the apparent protein-based oral liquid product of functional effect.
Specific embodiment
[embodiment 1]
A kind of preparation method of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function, including following step
It is rapid:
(1) preparation of compound protein peptide: egg-white powder 300g, degreased pupa powder 200g are dissolved in 10 times of amount distilled water, are used
Power 120W ultrasonication 10 minutes is first added 8000U/g papain and digests 3h under the conditions of 60 DEG C, uses 0.5mol/L
NaOH solution adjustment enzymatic hydrolysis pH value of solution adds 10000U/g alkali protease and digests 5h under the conditions of 60 DEG C to 8.0~8.5,
Boiling water bath enzyme deactivation 10min, enzymolysis liquid sock filtration, filtered fluid is added appropriate beta-cyclodextrin and stirs 30min, then with 8000r/
Min revolving speed is centrifuged 20min, takes 60 DEG C of supernatant to be concentrated in vacuo to the 1/8~1/10 of original volume, obtains compound protein peptide concentrate,
It is spare；
(2) preparation of water-soluble pearl calcium powder: pearl is clean, drying, crushing sieve with 100 mesh sieve, and weigh the addition of 20g pearl powder
2.5 times of amount lactic acid are aided with 250W ultrasonic treatment 20min while stirring in water-bath, then boil hydrolysis 2h, are stirred continuously, instead
It is filtered after answering, filtrate is individually stored；Filter residue adds appropriate amount of deionized water, with Ca (OH)2 PH value is adjusted to 7.0,0 .3g is added
Neutral proteinase digests 6h under the conditions of microwave power 400W, temperature 50 C, boils enzyme deactivation 10min, and enzymolysis liquid filtering merges acid
Filtrate and enzymatic hydrolysis filtrate are solved, is mixed, 50 DEG C of vacuum concentrations are at dry cream, and 40 DEG C of low temperature dryings, crush to get water solubility in an oven
Pearl powder；
(3) preparation of oral solution: citric acid 5g, sucrose 100g and step (2) the water-soluble pearl calcium powder prepared are dissolved in few
It measures in distilled water, the compound protein peptide concentrate that step (1) preparation is then added stirs evenly, fixed using 15MPa pressure homogeneous
Hold, it is filling, it is vented (70~80 DEG C, 10 min), sealing, 100 DEG C of sterilizing 10min are in water-bath to get oral solution finished product.
[embodiment 2]
A kind of preparation method of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function, including following step
It is rapid:
(1) preparation of compound protein peptide: egg-white powder 340g, degreased pupa powder 160g are dissolved in 10 times of amount distilled water, are used
Power 120W ultrasonication 10 minutes is first added 8000U/g papain and digests 4h under the conditions of 60 DEG C, uses 0.5mol/L
NaOH solution adjustment enzymatic hydrolysis pH value of solution adds 10000U/g alkali protease and digests 4h under the conditions of 60 DEG C to 8.0~8.5,
Boiling water bath enzyme deactivation 10min, enzymolysis liquid sock filtration, filtered fluid is added appropriate beta-cyclodextrin and stirs 30min, then with 8000r/
Min revolving speed is centrifuged 20min, takes 60 DEG C of supernatant to be concentrated in vacuo to the 1/8~1/10 of original volume, obtains compound protein peptide concentrate,
It is spare；
(2) preparation of water-soluble pearl calcium powder: pearl is clean, drying, crushing sieve with 100 mesh sieve, and weigh the addition of 25g pearl powder
2.5 times of amount lactic acid are aided with 250W ultrasonic treatment 20min while stirring in water-bath, then boil hydrolysis 3h, are stirred continuously, instead
It is filtered after answering, filtrate is individually stored；Filter residue adds appropriate amount of deionized water, with Ca (OH)2 PH value is adjusted to 7.0,0 .3g is added
Neutral proteinase digests 5h under the conditions of microwave power 400W, temperature 50 C, boils enzyme deactivation 10min, and enzymolysis liquid filtering merges acid
Filtrate and enzymatic hydrolysis filtrate are solved, is mixed, 50 DEG C of vacuum concentrations are at dry cream, and 40 DEG C of low temperature dryings crush to get water solubility in an oven
Pearl powder；
(3) preparation of oral solution: citric acid 5g, sucrose 100g and step (2) the water-soluble pearl calcium powder prepared are dissolved in few
It measures in distilled water, the compound protein peptide concentrate that step (1) preparation is then added stirs evenly, fixed using 15MPa pressure homogeneous
Hold, it is filling, it is vented (70~80 DEG C, 10 min), sealing, 100 DEG C of sterilizing 10min are in water-bath to get oral solution finished product.
[embodiment 3]
A kind of preparation method of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function, including following step
It is rapid:
(1) preparation of compound protein peptide: egg-white powder 360g, degreased pupa powder 140g are dissolved in 10 times of amount distilled water, are used
Power 120W ultrasonication 10 minutes is first added 8000U/g papain and digests 4h under the conditions of 60 DEG C, uses 0.5mol/L
NaOH solution adjustment enzymatic hydrolysis pH value of solution adds 10000U/g alkali protease and digests 5h under the conditions of 60 DEG C to 8.0~8.5,
Boiling water bath enzyme deactivation 10min, enzymolysis liquid sock filtration, filtered fluid is added appropriate beta-cyclodextrin and stirs 30min, then with 8000r/
Min revolving speed is centrifuged 20min, takes 60 DEG C of supernatant to be concentrated in vacuo to the 1/8~1/10 of original volume, obtains compound protein peptide concentrate,
It is spare；
(2) preparation of water-soluble pearl calcium powder: pearl is clean, drying, crushing sieve with 100 mesh sieve, and weigh the addition of 30g pearl powder
2.5 times of amount lactic acid are aided with 250W ultrasonic treatment 20min while stirring in water-bath, then boil hydrolysis 3h, are stirred continuously, instead
It is filtered after answering, filtrate is individually stored；Filter residue adds appropriate amount of deionized water, with Ca (OH)2 PH value is adjusted to 7.0,0 .3g is added
Neutral proteinase digests 6h under the conditions of microwave power 400W, temperature 50 C, boils enzyme deactivation 10min, and enzymolysis liquid filtering merges acid
Filtrate and enzymatic hydrolysis filtrate are solved, is mixed, 50 DEG C of vacuum concentrations are at dry cream, and 40 DEG C of low temperature dryings, crush to get water solubility in an oven
Pearl powder；
(3) preparation of oral solution: citric acid 5g, sucrose 100g and step (2) the water-soluble pearl calcium powder prepared are dissolved in few
It measures in distilled water, the compound protein peptide concentrate that step (1) preparation is then added stirs evenly, fixed using 15MPa pressure homogeneous
Hold, it is filling, it is vented (70~80 DEG C, 10 min), sealing, 100 DEG C of sterilizing 10min are in water-bath to get oral solution finished product.
The analysis of [embodiment 1-3] product strengthen immunity effect
Referring in the Ministry of Public Health " the function assessment assessment process and the method for inspection of health food " (2003) strengthen immunity functional experiment
Correlation test method give mouse oral stomach-filling daily, continuously according to this patent Products Show day dose 50ml/ people/day
Every immune indexes, control group distilled water stomach-filling are surveyed after 30d, test result see the table below:
The test of 1 mouse cell immune function of table, humoral immune function test result
Note: * P < 0.05, difference has statistical significance compared with the control group, similarly hereinafter.
2 mouse monokaryons of table-macrophage function test, NK cell activity assays result
Organism immunoregulation can be effectively improved it is found that oral solution prepared by this patent is taken as upper watch test result, have and increase
The function of strong immunity.
For the ordinary skill in the art, specific embodiment is only exemplarily described the present invention,
Obviously the present invention specific implementation is not subject to the restrictions described above, as long as use the inventive concept and technical scheme of the present invention into
The improvement of capable various unsubstantialities, or not improved the conception and technical scheme of the invention are directly applied to other occasions
, it is within the scope of the present invention.
Claims (5)
1. a kind of formula of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function, it is characterised in that every
1000 milliliters of oral solutions include: 300~360g of egg-white powder, 140~200g of degreased pupa powder, 20~30g of pearl powder, citric acid
5g, sucrose 100g.
2. a kind of preparation method of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function, it is characterised in that
The following steps are included:
(1) preparation of compound protein peptide: egg-white powder, degreased pupa powder are dissolved in 10 times of amount distilled water, power 120W is used
Ultrasonication 10 minutes, papain is first added and digests 3~4h under the conditions of 60 DEG C, with 0.5mol/L NaOH solution tune
Whole enzymatic hydrolysis pH value of solution adds alkali protease and digests 4~5h, boiling water bath enzyme deactivation 10min under the conditions of 60 DEG C to 8.0~8.5,
Enzymolysis liquid sock filtration, filtered fluid are added appropriate beta-cyclodextrin and stir 30min, are then centrifuged 20min with 8000r/min revolving speed,
It takes 60 DEG C of supernatant to be concentrated in vacuo to the 1/8~1/10 of original volume, obtains compound protein peptide concentrate, it is spare；
(2) preparation of water-soluble pearl calcium powder: pearl is clean, drying, crushing sieve with 100 mesh sieve, and weigh pearl powder according to the ratio and add
Enter 2.5 times of amount lactic acid and be aided with 250W ultrasonic treatment 20min while stirring in water-bath, then boils 2~3h of hydrolysis, constantly stir
It mixes, filters after reaction, filtrate is individually stored；Filter residue adds appropriate amount of deionized water, with Ca (OH)2 PH value is adjusted to 7.0, is added
Enter 0 .3g neutral protease enzymolysis, enzyme deactivation 10min is boiled after enzymatic hydrolysis, enzymolysis liquid filtering merges acidolysis filtrate and enzymatic hydrolysis filter
Liquid mixes, and 50 DEG C of vacuum concentrations are at dry cream, and 40 DEG C of low temperature dryings, crush to get water soluble pearl powder in an oven；
(3) water-soluble pearl calcium powder prepared by citric acid, sucrose and step (2) preparation of oral solution: is dissolved in a small amount of distillation
In water, the compound protein peptide concentrate that step (1) preparation is then added is stirred evenly, and using 15MPa pressure homogeneous, constant volume is filled
Dress is vented (70~80 DEG C, 10 min), sealing, and 100 DEG C of 10~15min of sterilizing are in water-bath to get oral solution finished product.
3. a kind of albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function as described in claim 1, special
Sign is, daily to recommend dose for 50mL/ people/day.
4. a kind of preparation of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function as described in claim 1
Method, which is characterized in that protease additive amount described in the step (1) is respectively 8000U/g papain, 10000U/g
Alkali protease.
5. a kind of preparation of the albumin amino acid pearl Ca oral liquid with immunological regulation healthcare function as described in claim 1
Method, which is characterized in that filter residue enzymatic hydrolysis condition after the hydrolysis of lactic acid described in the step (2) are as follows: microwave power 400W, temperature 50
DEG C, enzymatic hydrolysis 5~6h.
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Cited By (4)
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| CN111979285A (en) * | 2020-08-27 | 2020-11-24 | 浙江长生鸟健康科技股份有限公司 | Preparation method of pearl peptide chelated calcium |
| CN114343189A (en) * | 2022-01-18 | 2022-04-15 | 广东紫薇星健康产业有限公司 | Health albumin pearl calcium oral liquid with immunoregulation function and preparation method thereof |
| CN115251401A (en) * | 2022-06-17 | 2022-11-01 | 兆鑫堂(山东)生物科技有限公司 | Collagen peptide nutritional supplement composition beneficial to immunity improvement and application thereof |
| CN116042757A (en) * | 2022-12-07 | 2023-05-02 | 广东绍河珍珠有限公司 | Preparation method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder |
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