CN110463527A - The method for inducing balsam pear seedling stage anti-blight - Google Patents
The method for inducing balsam pear seedling stage anti-blight Download PDFInfo
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- CN110463527A CN110463527A CN201910773219.8A CN201910773219A CN110463527A CN 110463527 A CN110463527 A CN 110463527A CN 201910773219 A CN201910773219 A CN 201910773219A CN 110463527 A CN110463527 A CN 110463527A
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- balsam pear
- induction
- seedling stage
- blight
- disease
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Toxicology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of methods for inducing balsam pear seedling stage anti-blight, carry out induction processing to balsam pear seedling using amino-oligosaccharide, make balsam pear to wilt disease generation system resistance.Good Induced resistant effect is achieved using the method for present invention induction balsam pear seedling stage anti-blight, the diseased plant rate of its blank control is 100%, diseased plant rate after induction is 50.00%, disease index is 25.00, it is 70.39 that it, which lures anti-Disease resistance index, it realizes being greatly lowered for disease index, effectively controls the generation of bitter gourd wilt.In short, the present invention is a kind of method for inducing balsam pear seedling stage anti-blight simply, efficiently, safe, it is widely applied and balsam pear seedling stage can be improved to the resistance of wilt disease to reduce the generation of bitter gourd wilt, mitigate harm of the wilt disease to balsam pear.
Description
Technical field
Bitter gourd wilt technology technical field of the present invention more particularly to a kind of method for inducing balsam pear seedling stage anti-blight.
Background technique
Balsam pear becomes important characteristic melon vegetables due to its special medicinal health function and Peak output benefit.However,
Due to the long-term intensive cropping in the balsam pear place of production, Soil-sickness Problem becomes increasingly conspicuous, and the generation of especially wilt disease is spread rapidly.
According to investigations, the diseased plant rate of general lesion bitter gourd wilt is 12.30~56.75%, and grave illness area diseased plant rate reaches 85%
More than, or even it is all dead.Therefore, bitter gourd wilt has become an important factor for restricting industry development.
Currently, chemical prevention is little for the control efficiency of bitter gourd wilt, mainly carried out by modes such as soil disinfections,
But the harmful organism in soil is not only eliminated in disinfection, also affects the beneficial organism in soil, and disinfectant is used for a long time and can also
Soil physics structure is damaged, rhizosphere soil microenvironment and root growth are influenced.In addition, there is also workloads for disinfectant measure
Greatly, the features such as high production cost, and climate factor is affected, and cannot solve soil continuous cropping obstacle from the root.Biology is anti-
Controlling is safer Prevention Technique generally acknowledged at present, is promoted and applied in the country such as the U.S., China, but due to biological control medicament
It cannot be applied simultaneously with other chemical agents in many cases, effect performance is slower, answers so that biological control can't be always
Focus.
Inducing disease-resistant technology is another safely and effectively Prevention Technique, is activated by biology or abiotic component, is relied on
In the physically or chemically activation resistance processes of host plant itself, plant itself is made to generate " immune " reaction to external pathogen,
To obtain the resistance to pathogen, also known as systemic acquired resistance (Systemic Acquired Resistance, abbreviation
SAR).The substance for causing disease resistance is inducer, also known as exciton (Elicitor), these substances are at very low concentrations
It can be identified by host plant, induction plant generation system obtains resistance, and plant is finally made to obtain the ability for resisting external pathogen.
Cropper resistance is improved using inducer and effectively prevents corps diseases ability, it has also become control corps diseases
Important development direction.In " chitosan induces balsam pear to the resistance and its Exploration of Mechanism of wilt disease " text of Yang Ye, with 1%
Acetum prepares the CS2 liquid of 100 μ g/mL pH6.30, it is mixed into MK liquid with isometric culture solution, plant is placed in
MK liquid soaks root for 24 hours, the results showed that chitosan has certain effect to balsam pear anti-blight.But different inducers, different administration side
Formula, the Induced resistant effect having to the different diseases of Different Crop is multifarious, amino-oligosaccharide induction balsam pear is resisted at present withered
The research for disease of withering there is no report.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of simple, efficiently, safe induction balsam pear seedling stage anti-blights
Method, to realize that balsam pear resists the promotion of wilt disease ability.
In order to solve the above technical problems, the invention adopts the following technical scheme:
The method for inducing balsam pear seedling stage anti-blight, carries out induction processing to balsam pear seedling using amino-oligosaccharide, makes hardship
Melon is to wilt disease generation system resistance.
Balsam pear seedling is in 2 cotyledons and the phase is unfolded.
Amino-oligosaccharide is 1000 times of liquid of 5% amino-oligosaccharide.
Wilt disease is that Fusarium oxysporum balsam pear specialized form (Fusarium oxysporum f.sp.momordicae) causes
Bitter gourd wilt.
Induction processing uses pouring root mode.
Induction processing carries out 3 times, every plant of pouring root 50ml, induces 1 time every 3d,
Current bitter gourd wilt prevention and treatment there are aiming at the problem that, inventor establishes a kind of induction balsam pear seedling stage anti-blight
Method, induction processing is carried out to balsam pear seedling using amino-oligosaccharide, makes balsam pear to wilt disease generation system resistance.The method
It is the mode induced by using inducer, balsam pear itself is made to generate " immune " reaction to external pathogen, to obtains to withered
The resistance for disease of withering.By carrying out different inducers (0.5% chitosan, 6% oligosaccharide element, 5% amino-oligosaccharide), induction side
Formula (foliar spray, pouring root), induced concentration (800 times, 1000 times, 1500 times) etc. induce the exploration of disease-resistant condition, invention human hair
It is existing, good Induced resistant effect, the diseased plant of blank control are achieved using the method for present invention induction balsam pear seedling stage anti-blight
Rate is 100%, and the diseased plant rate after induction is 50.00%, disease index 25.00, and luring anti-Disease resistance index is 70.39, is realized
Disease index is greatly lowered, and effectively controls the generation of bitter gourd wilt.In short, the present invention is a kind of simple, efficient, safety
Induction balsam pear seedling stage anti-blight method, be widely applied and balsam pear seedling stage can be improved to the resistance of wilt disease to reduce
The generation of bitter gourd wilt mitigates harm of the wilt disease to balsam pear.
Detailed description of the invention
Fig. 1 is foliar spray Induced resistant effect figure.
Fig. 2 is pouring root inducing effect figure.
Fig. 3 is the influence diagram (0d-2d) that balsam pear root system is infected in amino-oligosaccharide induction to wilt.
Fig. 4 is the influence diagram (4d-10d) that balsam pear root system is infected in amino-oligosaccharide induction to wilt.
Fig. 5 is the influence diagram (14d-24d) that balsam pear root system is infected in amino-oligosaccharide induction to wilt.
For Fig. 3 into Fig. 5, left figure is amino-oligosaccharide induction group, and right figure is CK group.
Specific embodiment
One, induction balsam pear seedling stage anti-blight research
1. induction mode
(1) foliar spray induces
If 3 processing, clear water compare and (be shown in Table 1), is induced for the first time in 2 cotyledon expansion phases of balsam pear seedling, induce 1 every 3d
It is secondary, coinduction 3 times.Inducer is uniformly sprayed on to balsam pear blade face, L/ plants of 200 μ of spray amount with ulv spraying system.Induction terminates
For 24 hours afterwards with 1 × 106mL-1Fusarium oxysporum balsam pear specialized form bacterium spore suspension liquid irrigating root inoculation.15d is investigated after inoculation
Bitter gourd wilt incidence and Induced resistant effect.
1 foliar spray induction of table processing
| Processing | Inducer title | Spraying treatment concentration |
| Y-1 | 0.5% chitosan | 300 times of liquid |
| Y-2 | 6% oligosaccharide element | 800 times of liquid |
| Y-3 | 5% amino-oligosaccharide | 1500 times of liquid |
| CK | Clear water | - |
Wherein, inducer source is as follows:
0.5% chitosan: the production of Chengdu Mortopl development in science and technology Co., Ltd, aqua, active constituent content 0.5%.
6% oligosaccharide element: Hainan Zhengye Zhongnong Hi-Tech Industry Co., Ltd's production, aqua, active constituent content 6%.
5% amino-oligosaccharide: Hainan Zhengye Zhongnong Hi-Tech Industry Co., Ltd's production, aqua, active constituent content 5%.
(2) pouring root induces
2 cotyledon expansion phases of balsam pear Miao Yu induce for the first time, and every plant of pouring root 50ml is induced 1 time, coinduction 3 times every 3d.It lures
Pouring root is inoculated with after leading end for 24 hours.15d investigates bitter gourd wilt incidence and Induced resistant effect after inoculation.
2 pouring root induction of table processing
| Serial number | Inducer title | Root irrigation concentration |
| G-1 | 0.5% chitosan | 100 times of liquid |
| G-2 | 6% oligosaccharide element | 600 times of liquid |
| G-3 | 5% amino-oligosaccharide | 1000 times of liquid |
| CK | Clear water | - |
(3) test result
Test result is as shown in table 3 and table 3, and in the induction of foliar spray, the effect of amino-oligosaccharide is best, diseased plant rate
It is 30.83%, disease index 30.83, luring anti-Disease resistance index is 43.09；Chitosan, oligosaccharide element Induced resistant effect pay no attention to
Think.In pouring root induction, amino-oligosaccharide effect is best, diseased plant rate 50.00%, and disease index 25.00 lures disease-resistant finger
Number is 70.39；The inducing effect of chitosan and oligosaccharide element is undesirable.
3 inducer foliar spray Induced resistant effect of table
| Processing | Diseased plant rate % | Disease index | Induce Disease resistance index |
| Y-1 | 82.35 | 54.90 | -1.34 |
| Y-2 | 94.44 | 62.34 | -15.08 |
| Y-3 | 52.63 | 30.83 | 43.09 |
| CK | 87.50 | 54.17 | - |
4 pouring root inducing effect of table
| Processing | Diseased plant rate % | Disease index | Induce Disease resistance index |
| G-1 | 93.75 | 75.69 | 10.36 |
| G-2 | 91.67 | 87.96 | -4.16 |
| G-3 | 50.00 | 25.00 | 70.39 |
| CK | 100.00 | 84.44 | - |
2. the research of inducer induced concentration
It is screened according to early period, using pouring root abductive approach, studies amino-oligosaccharide and most preferably lure anti-concentration.Balsam pear Miao Yu 2
The cotyledon expansion phase induces for the first time, and every plant of pouring root 50ml is induced 1 time, coinduction 3 times every 3d.Pouring root connects after induction terminates for 24 hours
Kind.
5 amino-oligosaccharide of table lures anti-concentration to handle
6 various concentration amino-oligosaccharide inducing effect of table
As shown in table 6, under different induced concentrations, there is some difference for amino-oligosaccharide inducing effect, wherein amino-oligosacchride
The optium concentration of plain pouring root induction is 1000 times of liquid.
Embodiment 1
1. substrate seedling
Select excellent Varieties of Balsam Pear, carry out matrix hole plate seedling growth: using 55 DEG C of 10min that hot water treatment of seeds, clear water impregnates 8h, uses
Wet towel package is put into crisper, and 30 DEG C of vernalization, dibbling is into 32 hole seedling culture hole plates after seed shows money or valuables one carries unintentionally.
2. induction of the amino-oligosaccharide root irrigation to balsam pear seedling stage anti-blight
According to the above results, it by 5% amino-oligosaccharide, 1000 times of liquid, is carried out in 2 cotyledon expansion phases of balsam pear seedling stage
Pouring root induction, every plant of pouring root 50ml are induced 1 time, coinduction 3 times every 3d.Pouring root inoculation after induction terminates for 24 hours.Each processing sets 3
A repetition, 15 plants of balsam pears of each repetition, clear water compare (CK).After inoculation 15d investigate bitter gourd wilt incidence and
Induced resistant effect.
Result of study shows that amino-oligosaccharide has good effect to the induction of balsam pear seedling stage anti-blight.Amino is few
After sugared element pouring root induction, diseased plant rate 50.00%, disease index 25.00, luring anti-Disease resistance index is 70.39；Clear water control
Diseased plant rate is 100%, disease index 84.44.
3. amino-oligosaccharide induces the influence for infecting balsam pear to wilt
With 5% amino-oligosaccharide, 1000 times of liquid, pouring root induction, every plant of pouring root are carried out in 2 cotyledon expansion phases of balsam pear seedling stage
50ml is induced 1 time, coinduction 3 times every 3d.If not inducing only inoculation is control treatment (CK).0d, 2d after inoculation, 4d, 6d,
8d, 10d, 14d, 18d, 22d, 26d take balsam pear root tissue spare respectively.Using acid fuchsin staining (referring to remaining midwinter
(2005) method is slightly changed), root condition is infected in Olympus BX51 microscopically observation wilt.
As a result (Fig. 3 to Fig. 5) display, 6d is inoculated with after amino-oligosaccharide induces, begins with wilt disease in balsam pear root system
Bacterium is infected, and a small amount of mycelia is observed in 6-14d root system, and a large amount of mycelia occurs in 18d, and 22d starts spore occur, and 24d generates big
Measure spore.Studies have shown that the inducing action of amino-oligosaccharide can not prevent bitter gourd wilt bacterium mycelia from infecting root system, but
It can delay the time of infection of wilt, while the further development that mycelia can be prevented and the formation for inhibiting spore, success
The immune system for exciting plant pair wilt disease, greatly reduces disease incidence.
Claims (6)
1. a kind of method for inducing balsam pear seedling stage anti-blight, it is characterised in that lured using amino-oligosaccharide balsam pear seedling
Processing is led, makes balsam pear to wilt disease generation system resistance.
2. the method for induction balsam pear seedling stage anti-blight according to claim 1, it is characterised in that: at the balsam pear seedling
The phase is unfolded in 2 cotyledons.
3. the method for induction balsam pear seedling stage anti-blight according to claim 1, it is characterised in that: the amino-oligosaccharide
For 1000 times of liquid of 5% amino-oligosaccharide.
4. the method for induction balsam pear seedling stage anti-blight according to claim 1, it is characterised in that: the wilt disease is point
Bitter gourd wilt caused by fusarium oxysporum balsam pear specialized form.
5. the method for induction balsam pear seedling stage anti-blight according to claim 1, it is characterised in that: the induction processing is adopted
With pouring root mode.
6. it is according to claim 5 induction balsam pear seedling stage anti-blight method, it is characterised in that: it is described induction handle into
It row 3 times, every plant of pouring root 50ml, is induced 1 time every 3d.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110800569A (en) * | 2019-12-05 | 2020-02-18 | 太湖县旺顺种植农民专业合作社 | Snakegourd fruit planting method |
| CN111373903A (en) * | 2020-03-17 | 2020-07-07 | 广西壮族自治区农业科学院 | Method for removing fusarium wilt of bitter gourd seeds |
| CN115643988A (en) * | 2022-10-22 | 2023-01-31 | 广西壮族自治区农业科学院 | Screening method and application of bitter gourd fusarium wilt resistant germplasm |
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| CN115643988A (en) * | 2022-10-22 | 2023-01-31 | 广西壮族自治区农业科学院 | Screening method and application of bitter gourd fusarium wilt resistant germplasm |
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