CN110452919B - 一种截短的褐藻胶裂解酶Aly7B-CDII基因及其应用 - Google Patents
一种截短的褐藻胶裂解酶Aly7B-CDII基因及其应用 Download PDFInfo
- Publication number
- CN110452919B CN110452919B CN201910854192.5A CN201910854192A CN110452919B CN 110452919 B CN110452919 B CN 110452919B CN 201910854192 A CN201910854192 A CN 201910854192A CN 110452919 B CN110452919 B CN 110452919B
- Authority
- CN
- China
- Prior art keywords
- cdii
- aly7b
- alginate
- truncated
- alginate lyase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910854192.5A CN110452919B (zh) | 2019-09-10 | 2019-09-10 | 一种截短的褐藻胶裂解酶Aly7B-CDII基因及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910854192.5A CN110452919B (zh) | 2019-09-10 | 2019-09-10 | 一种截短的褐藻胶裂解酶Aly7B-CDII基因及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110452919A CN110452919A (zh) | 2019-11-15 |
CN110452919B true CN110452919B (zh) | 2021-05-14 |
Family
ID=68491528
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910854192.5A Active CN110452919B (zh) | 2019-09-10 | 2019-09-10 | 一种截短的褐藻胶裂解酶Aly7B-CDII基因及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110452919B (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111041013B (zh) * | 2019-12-31 | 2020-11-17 | 潍坊麦卡阿吉生物科技有限公司 | 一种褐藻胶裂解酶或果胶酶及其在协同降解褐藻方面的应用 |
CN114574472B (zh) * | 2020-12-02 | 2023-08-18 | 中国科学院大连化学物理研究所 | 一种重组多糖降解酶及其编码基因与应用 |
CN113308455B (zh) * | 2021-06-22 | 2022-06-07 | 南京工业大学 | 褐藻胶裂解酶AlyPL17、截短体及其应用 |
CN115786318B (zh) * | 2022-12-20 | 2024-05-07 | 中国海洋大学 | 褐藻胶裂解酶截短体Algt1及其应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3092247A1 (en) * | 2014-01-10 | 2016-11-16 | Matis ohf. | Thermostable alginate degrading enzymes and their methods of use |
CN105624137B (zh) * | 2014-11-18 | 2020-01-21 | 中国科学院大连化学物理研究所 | 一种褐藻胶裂解酶Algb及其编码基因和应用 |
CN106350531A (zh) * | 2016-10-24 | 2017-01-25 | 南京工业大学 | 一种褐藻胶裂解酶基因及其应用 |
CN106811454B (zh) * | 2017-02-28 | 2021-03-23 | 滕州市悟通香料有限责任公司 | 一种截短的重组褐藻胶裂解酶rAly1-T185N及其编码基因与应用 |
CN107287179A (zh) * | 2017-06-19 | 2017-10-24 | 五洲丰农业科技有限公司 | 一种褐藻胶裂解酶及其应用 |
CN107904223B (zh) * | 2017-12-26 | 2020-03-27 | 中国科学院天津工业生物技术研究所 | 一种褐藻胶裂解酶、分泌褐藻胶裂解酶的宿主细胞及其应用 |
CN108285900B (zh) * | 2018-04-12 | 2020-06-09 | 江南大学 | 一种重组褐藻胶裂解酶及其构建方法及应用 |
-
2019
- 2019-09-10 CN CN201910854192.5A patent/CN110452919B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN110452919A (zh) | 2019-11-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110452919B (zh) | 一种截短的褐藻胶裂解酶Aly7B-CDII基因及其应用 | |
CN109295043B (zh) | 一种褐藻胶裂解酶、其制备方法及应用 | |
CN112941089B (zh) | 褐藻胶裂解酶突变基因、褐藻胶裂解酶突变体、含该突变体的工程菌及构建方法和应用 | |
KR102011718B1 (ko) | 해조류로부터 포도당 및 라미나리올리고당을 생산하는 신규한 베타―글루코시데이즈 | |
CN112725319B (zh) | polyG底物特异性的褐藻胶裂解酶FaAly7及其应用 | |
CN114410611B (zh) | 昆布多糖降解酶OUC-BsLam26及其应用 | |
CN114480350B (zh) | 卡拉胶酶在降解κ-卡拉胶和红藻胶中的应用 | |
CN111235131B (zh) | 一种壳聚糖酶及其应用 | |
CN110643622A (zh) | 一种褐藻胶裂解酶基因及其应用 | |
CN107603967B (zh) | 一种壳聚糖酶csn4及其编码基因和应用 | |
CN110144341B (zh) | 海藻酸裂解酶突变体 | |
CN111187764B (zh) | 一种深海来源的壳聚糖酶csn5及其编码基因和应用 | |
CN111334488B (zh) | 昆布多糖酶ouc-l1及其编码基因与应用 | |
CN105647888B (zh) | 内切几丁质酶及其编码基因和在生产几丁二糖中的应用 | |
CN114196655B (zh) | 耐热性昆布多糖降解酶OUC-SaLam66及其应用 | |
CN114457057B (zh) | 一种壳聚糖酶突变体及其应用 | |
CN111849949B (zh) | 一种甘露糖醛酸c-5差向异构酶/海藻酸裂解酶编码基因及酶和制备与应用 | |
CN110951708B (zh) | 一种n-乙酰氨基葡萄糖脱乙酰酶及其编码基因与应用 | |
CN113046378A (zh) | 一种内切褐藻胶裂解酶、其编码基因及应用 | |
CN105647898A (zh) | 一种海洋褐藻酸裂解酶及其表达基因与应用 | |
CN109762798A (zh) | 一种巴伦葛兹类芽孢杆菌壳聚糖酶的制备方法与应用 | |
CN113308455B (zh) | 褐藻胶裂解酶AlyPL17、截短体及其应用 | |
CN114015735B (zh) | 一种蔗糖磷酸化酶和葡萄糖异构酶级联催化合成黑曲霉二糖的方法 | |
CN110982831B (zh) | 基因AlgL23具有冷适应性的用途 | |
CN110713997B (zh) | 一种降解产物均一的琼胶酶及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhu Benwei Inventor after: Ni Fang Inventor after: Hu Fu Inventor after: Yao Zhong Inventor after: Sun Yun Inventor after: Li Qian Inventor before: Ni Fang Inventor before: Zhu Benwei Inventor before: Hu Fu Inventor before: Yao Zhong Inventor before: Sun Yun Inventor before: Li Qian |
|
GR01 | Patent grant | ||
GR01 | Patent grant |