CN1104440A - Production of fodder protein by fermenting filtered mud - Google Patents
Production of fodder protein by fermenting filtered mud Download PDFInfo
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- CN1104440A CN1104440A CN93121183A CN93121183A CN1104440A CN 1104440 A CN1104440 A CN 1104440A CN 93121183 A CN93121183 A CN 93121183A CN 93121183 A CN93121183 A CN 93121183A CN 1104440 A CN1104440 A CN 1104440A
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- fermentation
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- filter mud
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
This invention refers to a prodn. method for fodder protein by fermenting filtered mud. The process includes selecting black mould and tropic pseudohypha yeast which is able to grow in the filtered mud; mix-fermanting on the solid media after single strain liquid fermanting in the deep layers; solid fermanting carried out in the solid ventilating fermantation machine; stoving to dry carried out in the flowed oven drying machine with hot air dewatering instantaneously at the temp. <50 deg.C for <2 s. This process which is suitable for industrilized continous fermantation prodn., is simple, realiable, low cost, high efficiency, and it not only solves the problem of environmental pollution, but also develops a new fodder protein resources.
Description
The present invention relates to a kind of process of fermenting and producing forage protein, it is not only applicable to filter mud as raw materials for production, and also can be suitable for bean dregs, vinasse, peanut cake is that raw material is produced forage protein.
As everyone knows, a large amount of mud waste things can appear when adopting cane sugar manufacture, 10 tons of sucrose of every production just have 1 ton dry filter mud, and this dry filter mud comprises sucrose 16-22%, crude fat 8.5%, thick dimension fine 8.2%, crude protein 13.3%, calcium 5.6%, phosphorus 0.93%, sulphur 2.1% also has a spot of magnesium, zinc, potassium, sodium, manganese, cobalt, copper, iron etc.At present domestic all is filter mud to be piled up allow the base manure as fruit tree uses after its spontaneous fermentation, environment is caused to a certain degree pollution, I once proposed " utilizing filter mud to produce the method for edible mushroom " in Chinese patent CN87102933 number for this reason, adopt this method that filter mud is turned waste into wealth, minimizing is to the pollution of environment, the edible mushroom that produces can be edible, but its application has certain limitation, popularize certain degree of difficulty in an all-round way.
Task of the present invention is that a kind of technology of utilizing filter mud fermentation to produce forage protein will be provided, it is applicable to the production of industrialization continuous fermentation method, its technology is simple, reliable, equipment investment is few, the productivity effect height has not only been administered the pollution of environment, and has developed new forage protein source, for surviving the winter of ruminant domestic animal opened up a new grain source, for " transferring Southern grain to the North " engineering that realizes China's feed is opened up an approach.
Task of the present invention is finished in such a way, this production technology adopted can be in filter mud well-grown aspergillus niger and candida tropicalis, using liquid, solid combined ferment, promptly use mixed culture fermentation on solid medium after single bacteria liquid submerged fermentation, thereby obtain to contain crude protein and reach 30%, and contain the forage protein of abundant vitamin, digestive ferment and an amount of inorganic salts, solid fermentation is to carry out in the ventilating fermentation machine, in order to help fermentation, it is partially mixed that nitrogenous source adopts, and the form that adds of stream is carried out in batches.Consider from the chemical composition of filter mud that simultaneously it is a height sugar amount matrix, and contain and definite by orthogonal test aspect nitrogenous source, nitrogen amount proportioning, can supply the carbon of fermentation for the various inorganic salts of fermentation that nitrogen is than rational solid fermentation culture medium thereby set up.Following mask body is introduced its production technology:
One, the making of culture medium
1, yeast slant medium: the raw material (not hopping) with fermentation beer, be diluted to 12-15 Berlin, add 1000 milliliters in water, add 15 in agar, dissolve the back packing, sterilized 20 minutes for 15 pounds.
2, mould slant medium: get filter mud 200 gram, wheat skin 20 grams, soya-bean cake powder 20 grams add 1000 milliliters in water, soak 1 hour, boil 20 minutes, cross leaching juice and are diluted to 1000 milliliters and add agar 15 grams, dissolve the back packing, and pH value is taken from right value, sterilizes 1 hour for 15 pounds.
3, yeast fluid nutrient medium: get bright filter mud 200 grams, add 1000 milliliters in water, agitation and filtration is got 5 ° of BX brewer's worts of juice and equivalent and is mixed, and pH value is taken from right value, sterilizes 20 minutes for 15 pounds.
4, mould fluid nutrient medium: get bright filter mud 200 grams, wheat skin 20 grams, soya-bean cake powder 20 grams add 1000 milliliters in water, soak 1 hour, boil 20 minutes, cross leaching juice and are diluted to 1000 milliliters, and pH value is taken from right value, sterilizes 20 minutes for 15 pounds.
5, solid fermentation culture medium: get bright filter mud (moisture 65%) and account for 90%, soya-bean cake powder 4%, wheat skin 4%, urea 1%, ammonium sulfate 1%, pH value are taken from right value, and moisture content transfers to 60%, 80 ℃ of sterilization 20 minutes.
Two, the female making of planting in inclined-plane: place 30 ℃ of incubators to cultivate 48 hours in yeast, mould places 30 ℃ of incubators to cultivate 6 days.
Three, liquid shaking bottle operation: it is that 220 rev/mins shaking table was cultivated 24 hours under 30 ℃ of room temperatures that yeast is placed rotating speed; Mould is placed 220 rev/mins shaking table, cultivated 36 hours for 30 ℃.
Four, seed tank culture: yeast is placed 220 rev/mins of shaking tables, and room temperature is 30 ℃, and throughput is 1: 0.8, cultivates 36 hours; Mould is in rotating speed is 330 rev/mins shaking table, and 30 ℃, throughput is 1: 0.8, cultivates 36 hours.
Five, solid fermentation: solid sterilization is cooled to 35 ℃ then and enters in the aerobic fementation machine and be mixed in 30 ℃ in fermentation 48 hours with yeast, mould.
Six, oven dry, packing: product carries out in the fluidised form dryer, utilizes the hot blast instant dehydration, controls whole drying course and is no more than 2 seconds, and temperature is no more than 50 ℃ from start to finish.
Adopt above-mentioned explained hereafter forage protein, destructiveness to effective ingredients such as protein, vitamin, digestive ferments is few, crude protein can reach 31%, amylase activity is greater than 3000, black mold has effectively comprehensively enzyme system, and product also has the cellulase system of a great deal of, zytase, protease etc. can be used as a kind of desirable poultry and livestock feed albumen.
Claims (3)
1, a kind of process of utilizing filter mud fermentation to produce forage protein, it is characterized in that: select for use can be in filter mud well-grown aspergillus niger and candida tropicalis, adopt after single bacteria liquid submerged fermentation mixed culture fermentation process on solid medium, and solid fermentation is to carry out in solid ventilating fermentation machine, in order to help fermentation, nitrogenous source is taked partially mixed, the form that adds of stream is carried out in batches, oven dry is to utilize the hot blast instant dehydration in the fluidised form dryer, control whole drying course and be no more than 2 seconds, temperature is no more than 50 ℃ all the time.
2, produce the process of forage protein by the filter mud fermentation that utilizes of claim 1 defined, it is characterized in that: determining by orthogonal test can be for the carbon of filter mud fermentation, nitrogen than rational solid fermentation culture medium.
3, produce the process of forage protein by the filter mud fermentation that utilizes of claim 1 defined, it is characterized in that: earlier yeast and mould are carried out liquid shaking bottle and seed tank culture, then solid is sterilized, enter after being cooled to 35 ℃ in the aerobic fementation machine and mix, in 30 ℃ of fermentations 48 hours with yeast, mould.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN93121183A CN1051445C (en) | 1993-12-28 | 1993-12-28 | Production of fodder protein by fermenting filtered mud |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN93121183A CN1051445C (en) | 1993-12-28 | 1993-12-28 | Production of fodder protein by fermenting filtered mud |
Publications (2)
Publication Number | Publication Date |
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CN1104440A true CN1104440A (en) | 1995-07-05 |
CN1051445C CN1051445C (en) | 2000-04-19 |
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Application Number | Title | Priority Date | Filing Date |
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CN93121183A Expired - Fee Related CN1051445C (en) | 1993-12-28 | 1993-12-28 | Production of fodder protein by fermenting filtered mud |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102960539A (en) * | 2012-12-04 | 2013-03-13 | 广西壮族自治区分析测试研究中心 | High-protein microbiological feed and preparation method thereof |
CN103228153A (en) * | 2010-10-08 | 2013-07-31 | 甜糖(曼海姆/奥克森富特)股份公司 | Colloid product, method for producing same and use thereof |
CN104171294A (en) * | 2014-07-04 | 2014-12-03 | 中国农业科学院兰州畜牧与兽药研究所 | Method for preparing solid fermented protein feed |
CN104222693A (en) * | 2014-10-11 | 2014-12-24 | 来光业 | Pig feed and producing method thereof |
CN109730192A (en) * | 2019-03-06 | 2019-05-10 | 广西壮族自治区农业科学院 | Utilize the method for bagasse production protein feed |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1078215A (en) * | 1992-04-30 | 1993-11-10 | 深圳市环境保护设备工程公司 | The treatment process of molasses alcohol waste liquid |
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1993
- 1993-12-28 CN CN93121183A patent/CN1051445C/en not_active Expired - Fee Related
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103228153A (en) * | 2010-10-08 | 2013-07-31 | 甜糖(曼海姆/奥克森富特)股份公司 | Colloid product, method for producing same and use thereof |
CN103228153B (en) * | 2010-10-08 | 2016-05-25 | 甜糖(曼海姆/奥克森富特)股份公司 | Colloid product and manufacture method thereof and application |
US9410215B2 (en) | 2010-10-08 | 2016-08-09 | Sudzucker Aktiengesellschaft Mannheim/Ochsenfurt | Colloid product, method for producing same and use thereof |
CN102960539A (en) * | 2012-12-04 | 2013-03-13 | 广西壮族自治区分析测试研究中心 | High-protein microbiological feed and preparation method thereof |
CN102960539B (en) * | 2012-12-04 | 2015-04-15 | 广西壮族自治区分析测试研究中心 | High-protein microbiological feed and preparation method thereof |
CN104171294A (en) * | 2014-07-04 | 2014-12-03 | 中国农业科学院兰州畜牧与兽药研究所 | Method for preparing solid fermented protein feed |
CN104222693A (en) * | 2014-10-11 | 2014-12-24 | 来光业 | Pig feed and producing method thereof |
CN109730192A (en) * | 2019-03-06 | 2019-05-10 | 广西壮族自治区农业科学院 | Utilize the method for bagasse production protein feed |
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CN1051445C (en) | 2000-04-19 |
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