CN101632411B

CN101632411B - Method for preparing high-quality rapeseed protein containing conjugated linoleic acid by multi-strain fermentation

Info

Publication number: CN101632411B
Application number: CN200910091010XA
Authority: CN
Inventors: 陈志敏
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-08-20
Filing date: 2009-08-20
Publication date: 2012-07-11
Anticipated expiration: 2029-08-20
Also published as: CN101632411A

Abstract

The invention discloses a method for preparing high-quality rapeseed protein containing conjugated linoleic acid by multi-strain mixed fermentation, which belongs to the field of feed resources. The method comprises the following steps: adding bran, carbohydrates, vegetable fat and water to the rapeseed protein; uniformly mixing and stirring the materials and sterilizing the materials; then inoculating a strain culture solution of lactobacillus plantara, bacilli and mould fungi for generating the conjugated linoleic acid to a rapeseed cake (dreg) for fermentation by controlling temperature. The rapeseed protein prepared by the method has a detoxification rate being higher than 85 percent and generates a high-quality protein raw material containing the conjugated linoleic acid, small peptides and amino acids through the fermentation processing of the lactobacillus plantara, the bacilli and the mould fungi, thereby effectively relieving the difficulty of being short of protein feeds of China.

Description

The multi-strain fermentation preparation contains the method for CLA high-quality rapeseed protein

Technical field

The invention belongs to the feed resource technical field, relate to the method that a kind of fermented by mixed bacterium preparation contains CLA high-quality rapeseed protein.

Background technology

China's rape cake (dregs of rice) output is abundant, produces more than 600 ten thousand tons per year.The accessory substance that rape cake (dregs of rice) is a vegetable seed after squeezing or extraction deoil processing, the accessory substance after squeezing is a rape cake, the accessory substance after extraction is processed is a rapeseed dregs.Its potential nutritive value and defatted soybean meal are equally matched; Main nutritional characteristic is: the contained thick protein of (1) rape cake (dregs of rice) is generally about 35-42%; To form from the amino acid of protein. the protein nutritive value of rape cake (dregs of rice) slightly is superior to soybean cake; The amino acid balance situation is better, is the very high protein of a kind of nutritive value.(2) rape cake (dregs of rice) contains more mineral matter, and calcium, iron, manganese, phosphorus, selenium and magnesium can be provided.(3) compare with soybean cake, rape cake (dregs of rice) contains more choline, biotin, nicotinic acid, Cobastab, and soybean cake contains more pantothenic acid.(4) crude fiber content of rape cake (dregs of rice) is higher, be about about 11%, and soybean cake has only 7%, and present solution is the consumption of restriction rape cake in feed, generally in feed, adds being no more than 20%.(5) contain toxicant or anti-nutrient substances such as glucosinolate and phytic acid, tannin, sinapine in the rape cake (dregs of rice).To the nutritional characteristic of rapeseed protein, if can be apt to add utilization, make full use of this native protein resource to it, can elongate industrial chain, improve the economic benefit of multi-form agriculture, can effectively alleviate the nervous problem of China's protein resource again.

A major reason that influences the rapeseed protein utilization is to contain toxicant and ANFs in the rape cake (dregs of rice), wherein main glucosinolate that is and phytic acid.The poison-removing method of rape cake (dregs of rice) is mainly physics and chemistry detoxicity method and biology detoxicity method both at home and abroad at present, the physico-chemical method cost is high, and seriously polluted, loss of nutritional ingredients is big, has bigger defective.Biological method is divided into enzyme process and microbe fermentation method; Enzyme process is to handle rape cake (dregs of rice) with phytase or zytase; Microbe fermentation method is to utilize microorganism directly to divide toxicide, compares with other method, has advantages such as mild condition, cost is low, loss of nutritional ingredients is little.

Anticancer, functions such as promotion is grown, inhibition accumulation of fat, enhancing body immunocompetence that CLA has can improve pig, the fowl speed of growth and lean meat percentage, are the research focuses of subjects such as current medicine, health food and Animal nutrition.But because CLA costs an arm and a leg, it is too high to add cost in the feed, uses few in the production practices.Therefore, the animal nutrition and feed scholar is seeking other suitable, cost effective method that improves cla levels in the livestock products.

The present invention passes through multiple bacteria compound fermentation; Effectively reduce the content of middle noxious material of rape cake (dregs of rice) and ANFs on the one hand; Use the Lactobacillus plantarum that can produce CLA on the other hand; Production contains the functional rapeseed protein of CLA, peptide and several amino acids, does not still have report at home and abroad.

Summary of the invention

Technical problem to be solved by this invention is: be intended to solve the high problem of noxious material content in the rapeseed protein; Improve the rapeseed protein nutritional labeling; And provide the fermenting process of preparing of utilizing Lactobacillus plantarum to contain the method for CLA high-quality rapeseed protein; To improve the addition of rapeseed protein in feed, alleviate the nervous problem of protein resource.

The method that this mixed fermentation method preparation contains CLA high-quality rapeseed protein is: earlier Lactobacillus plantarum, bacillus and mould are spread cultivation in batches; After again the rapeseed protein impurity elimination being pulverized; In the rapeseed protein powder, add wheat bran, carbohydrate, vegetable fat and water; Behind the mixing and stirring sterilization, Lactobacillus plantarum, bacillus and fungal strain nutrient solution are inoculated in the rape cake (dregs of rice) temperature controlled fermentation.

Below in conjunction with instantiation the present invention is further specified

The specific embodiment:

1, culture of strains

1) slant activation is cultivated: potato 100g, add 500ml water boil 30min again moisturizing to 500ml, 10g glucose, 10g agar.28 ℃ of constant temperature culture 48h.

2) seed liquor preparation: get the fresh slant strains of 2 rings and insert seed culture fluid, 28 ℃ of constant temperature shaking tables are cultivated 48h, rotating speed 250r/min.

2, with seed fermentation liquid solid fermentation substrate cultivation base is carried out solid fermentation

Rapeseed protein is evenly become basic substrate with wheat bran, corn flour by 70: 20: 10 mixed, and add vegetable fat in the ratio of 1%-3%, natural pH value, autoclave sterilization.Add seed fermentation liquid when being cooled to 30-34 ℃, inoculum concentration is 3-6%, leaves standstill and cultivates 48-72 hour.

3, oven dry and pulverizing obtain product

With the solid fermentation product of cultivating, natural airing to moisture content is lower than 12% back pulverizing and processes finished product.

Claims

1. a multi-strain fermentation prepares the method that contains CLA high-quality rapeseed protein, it is characterized in that:

1) earlier at potato 100g; Add 500ml water boil 30min again moisturizing to 500ml; 10g glucose, in the culture medium of 10g agar under 28 ℃ condition constant temperature culture 48 hours Lactobacillus plantarum, bacillus and mould carried out slant activation cultivate, again slant strains is inserted seed culture fluid; Under 28 ℃, the condition of constant temperature shaking speed 250r/min, cultivated 48 hours, Lactobacillus plantarum, bacillus and mould are carried out batch spread cultivation;

2) in the sterilization rape cake of removing foreign material or rapeseed dregs, inoculate Lactobacillus plantarum, bacillus and mould: rape cake or rapeseed dregs impurity elimination are pulverized back and wheat bran, corn flour, vegetable fat and water mixing and stirring; Sterilization back sprays that inoculation is above-mentioned to spread cultivation and diluted Lactobacillus plantarum, bacillus and mould bacteria culture fluid, is that 3-6%, the fermentation temperature of rape cake or rapeseed dregs amount is that 30-34 ℃, fermentation time are 48-72 hour anaerobic condition bottom fermentation in inoculum concentration.

2. a kind of multi-strain fermentation preparation according to claim 1 contains the method for CLA high-quality rapeseed protein, it is characterized in that: the substrate proportioning: rape cake or rapeseed dregs: wheat bran: corn flour=70: 20: 10.

3. a kind of multi-strain fermentation preparation according to claim 2 contains the method for CLA high-quality rapeseed protein; It is characterized in that: said vegetable fat is sunflower oil, rapeseed oil or peanut oil, and the vegetable fat addition is the 1-3% of rape cake or rapeseed dregs, wheat bran, corn flour total amount.

4. a kind of multi-strain fermentation preparation according to claim 1 contains the method for CLA high-quality rapeseed protein, and it is characterized in that: bacterial classification is Lactobacillus plantarum, bacillus and mould; During inoculation, the bacterial classification addition is the 3%-6% of rape cake or rapeseed dregs amount; The microbial fermentation time is 48-72 hour; Fermentation temperature is 30-34 ℃; The substrate moisture is 50%.