CN110438024B - Bacillus subtilis strain and application thereof - Google Patents
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Abstract
The invention discloses a Bacillus subtilis strain and application thereof, belonging to the technical field of applied biology, wherein the strain is Bacillus subtilis strain QS1, the preservation number of the strain is CGMCC NO.17314, the invention also discloses a Bacillus subtilis preparation, the preparation comprises Bacillus subtilis strain QS1, natural perlite powder and maltodextrin, the natural perlite powder is used as a Bacillus subtilis spray carrier in the preparation process, and the aerobic biochemical reaction of the Bacillus subtilis in a septic tank can be favorably carried out.
Description
Technical Field
The invention belongs to the technical field of applied biology, and particularly relates to a bacillus subtilis strain and application thereof.
Background
The septic tank is a device for treating excrement and filtering and precipitating, and the principle of the septic tank is that condensate is decomposed at the bottom of the septic tank, and hydrated objects on the upper layer flow away after entering a pipeline, so that the pipeline is prevented from being blocked, and sufficient time is provided for hydrolyzing the solidified objects (garbage such as excrement). The septic tank refers to a small-sized treatment structure for carrying out cellular precipitation on domestic sewage and carrying out anaerobic digestion on sludge.
The septic tank plays an important role as a traditional environment-friendly facility with more than one hundred years of history, but with the progress of society and the development of urban modernization, the problem deviating from the social development also appears, and urgent solutions are needed to be solved, which are mainly expressed in that: 1) The quality of the discharged water is unstable and difficult to adjust in the process, the use effect is poor, and the use scheme is difficult to adjust for different water quality requirements such as irrigation water, discharged water and the like according to local conditions. 2) The disposal problem of the sludge is as follows: according to the technical standard of septic tanks, the sludge cleaning time of the septic tank is generally 90 days, 180 days and 360 days, and the cleaned sludge can be used as organic fertilizer or be subjected to harmless treatment. However, with the wide use of chemical fertilizers and the development of society, the amount of used manure fertilizers is less and less, meanwhile, the sludge treatment industry in China is seriously lagged, the harmless treatment of manure sludge is insufficient, 13.6 million people in China generate over hundred million tons of manure sludge every year, and according to the data of statistical bureau, the harmless treatment of manure sludge in 2012 is only 801.4 million tons. The secondary pollution is caused by the clear drawing and transfer of a large amount of fecal sludge. Therefore, if the problem of harmless disposal of the sludge in the septic tank cannot be solved, the sludge in the septic tank can become a pollution source of water pollution, and the problem of the septic tank is an environmental protection problem related to the treatment effect of the water pollution. 3) The scum amount is large, the channel is easy to block, and the discarding rate is high: septic tank deposit mud, the bottom is deposit mud, and the top layer is floating mud, and the centre is suspension mud and sewage, and the septic tank is separated for two septic tanks or three septic tanks of check by the partition wall, crosses the water entrance to a cave on the partition wall and is located the middle part of septic tank partition wall, if the septic tank does not in time clearly draw, surpasss the height of crossing the water entrance to a cave when septic tank deposit mud, and mud will block up the entrance to a cave, causes the septic tank to block up. The blockage problem of the septic tank seriously influences the normal life of users, and is the application problem of the septic tank to be solved urgently. 4) Produce gas a lot, the potential safety hazard is high: the anaerobic fermentation of the excrement in the septic tank can generate a large amount of methane, ammonia and hydrogen sulfide gas, and the methane in the septic tank can explode when meeting open fire when reaching a certain concentration, so that the septic tank is very dangerous.
Researches show that the bacillus subtilis is a multifunctional microorganism, is widely applied to sewage treatment and bio-fertilizer fermentation or fermentation bed manufacturing, and is a multifunctional microorganism. Related applications of Bacillus subtilis in treatment of septic tanks are disclosed in the prior art, for example, a Chinese patent application number of 201710768348.9, an application with a publication date of 2017, 11 months and 07 days, discloses a Bacillus subtilis strain and application thereof, and provides a Bacillus subtilis strain and application thereof in preparation of a deodorant microbial inoculum. The preservation number of the strain in China general microbiological culture Collection center (CGMCC) is as follows: CGMCC No.13714, the technical scheme adopted by the application is as follows: inoculating bacillus subtilis CGMCC13714 in an activation culture medium to culture to obtain an activation strain, and inoculating the activation strain in an amplification fermentation culture medium to culture in an amplification way to obtain a bacterial liquid; inoculating the bacterial liquid into excrement and keeping the temperature at 20-35 ℃. The bacillus subtilis can be applied to biological deodorization of excrement, and has stable deodorization effect and small influence of temperature; can effectively deodorize by an in-situ deodorization method, has simple and easy operation, does not need matched equipment and technology, and has small investment scale. Although the bacillus subtilis CGMCC13714 of the application can deodorize the original taste, the scum quantity caused by grease substances is large aiming at a large amount of sludge and cellulose substances in a septic tank, and the problem of blockage of the septic tank is difficult to solve.
Aiming at the research that the septic tank can realize the complete degradation of the excrement, the Chinese patent application No. 201810847678.1 with the publication date of 2019.01.01 discloses a method for realizing the non-cleaning and non-drawing of the septic tank by three-step treatment by using a microbial agent, the method comprises the steps of degrading grease in the septic tank by using bacillus subtilis and bacillus licheniformis with strong grease degradation capability, degrading starch bacillus and lactobacillus rhamnosus with strong organic matter degradation capability by using bacillus amyloliquefaciens with strong organic matter degradation capability, and synchronously nitrifying and denitrifying by using acinetobacter oleophilus with heterotrophic nitrification-aerobic denitrification capability by using the three-step method, so that the biological degradation and the complete conversion of the excrement in the septic tank can be realized, and the methane generation in the septic tank can be realized, deodorized and the non-cleaning and non-drawing of the septic tank can be realized. Although the method of the application can thoroughly and effectively remove the excrement and the cleaning-free effect is realized, the single-type microorganism strain has single material processing capacity, so that multiple types of microorganisms are required to be used for simultaneous processing, the cost is high, the three steps are required to be carried out in the using process, and the treatment of using objects (farmers) of rural septic tanks is not facilitated.
Based on the defects of the prior art, the invention is needed to invent a biological agent for septic tank treatment, which has good treatment effect and is simple to use.
Disclosure of Invention
1. Problems to be solved
Aiming at the problem of single microbial treatment effect in the prior art for septic tank treatment, the invention provides bacillus subtilis QS1 with better decomposition capability on protein, fiber substances and oil substances, and further provides a preparation for aerobic treatment by using the bacillus subtilis QS1.
2. Technical scheme
In order to solve the problems, the technical scheme adopted by the invention is as follows:
the invention provides a Bacillus subtilis strain QS1 which is preserved in China general microbiological culture Collection center (CGMCC); the preservation number is: CGMCC No.17314; the address of the depository: the postcode of the microbiological research institute of Chinese academy of sciences, in the sunward area of Beijing: 100101; the preservation date is as follows: year 2019, month 3, day 08.
The bacillus subtilis CGMCC No.17314 is screened from grassland soil, and has the characteristics of: the strain cells are in a long rod shape, and spores are in an oval shape; the colony surface is rough and opaque, is in a dirty white color, can form wrinkle when cultured in a liquid, and can survive in a meta-acid or meta-alkali environment. The physiological and biochemical properties of the bacillus subtilis CGMCC17314 are shown in Table 1:
TABLE 1 physiological and biochemical properties of Bacillus subtilis CGMCC17314
The invention provides a bacillus subtilis preparation which comprises the bacillus subtilis strain.
As a further improvement of the invention, the Bacillus subtilis preparation comprises Bacillus subtilis strain QS1, natural perlite powder and maltodextrin.
The maltodextrin is used for rapid nutritional supplementation when the bacillus subtilis revives from a dormant state.
As a further improvement of the invention, the bacillus subtilis preparation comprises bacillus subtilis strain QS1, natural perlite powder, maltodextrin and composite biological enzyme freeze-dried powder, and the composite biological enzyme freeze-dried powder is used for activating bacillus subtilis.
The preparation method of the composite biological enzyme freeze-dried powder comprises the following steps: step A: carrying out solid-liquid separation on the pig manure to prepare a pig urine stock solution; and B: putting the pig urine stock solution into reaction barrels, and treating according to an A/A/O process in sequence, wherein micro-nano aeration devices are respectively arranged in the first three reaction barrels; step C: repeating the A/A/O process for at least 3 times on the pig urine stock solution, feeding the pig urine stock solution into a precipitation filter vat, filtering to obtain a compound biological enzyme, wherein the content of microorganisms in the compound biological enzyme is lower than 1%, and preparing the compound biological enzyme into compound biological enzyme freeze-dried powder.
As a further improvement of the invention, the effective viable count of the bacillus subtilis preparation is 1.0 multiplied by 10 7 ～1.0×10 10 CFU/g。
As a further improvement of the invention, the particle size of the natural perlite powder is 10 μm-2 mm.
As a further improvement of the invention, the preparation method of the bacillus subtilis preparation comprises the following steps:
1) Taking a small amount of pure bacteria from the preserved bacillus subtilis inclined plane, and inoculating the pure bacteria to a flat plate for activation;
2) Carrying out a fermentation experiment by using a small test device to obtain a bacterial liquid;
3) Spray drying the fermented bacterium liquid to obtain bacterium powder, wherein a spray carrier adopted in the spray drying is natural perlite powder;
4) Mixing the obtained bacterial powder with maltodextrin.
As a further improvement of the invention, the preparation method of the bacillus subtilis preparation comprises the following steps:
1) Taking a small amount of pure bacteria from the preserved bacillus subtilis inclined plane, and inoculating the pure bacteria to a flat plate for activation;
2) Carrying out a fermentation experiment by using a small test device to obtain a bacterial liquid;
3) Spray drying the fermented bacterium liquid to obtain bacterium powder, wherein a spray carrier adopted in the spray drying is natural perlite powder;
4) Mixing the obtained bacterial powder with maltodextrin and composite biological enzyme freeze-dried powder.
As a further improvement of the invention, the bacillus subtilis QS1 and the bacillus subtilis preparation are used for septic tank treatment, sewage treatment and facility blockage treatment.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
(1) The bacillus subtilis strain is obtained by screening from grassland soil, is nonpathogenic bacteria, is harmless to people and livestock, does not cause harm to the surrounding environment and ecological balance, and accords with biosafety regulations.
(2) The bacillus subtilis strain has strong decomposition capability aiming at various components such as protein, grease and fiber in excrement, can solve the problem that the septic tank is discarded due to the blockage of a channel by a large amount of sludge and scum when being applied to the treatment of the septic tank, simultaneously avoids the potential safety hazard problem caused by methane and hydrogen sulfide gas generated by anaerobic fermentation due to the blockage of a pipeline, can be widely applied to the treatment of residential districts, public toilets and rural septic tanks, can also be used for solving the blockage of facilities, and has wide application range.
(3) The bacillus subtilis strain has better decomposition capability aiming at urine protein, on one hand, the bacillus subtilis strain generates alkaline protease by bacterial exoenzyme to decompose urinary calculus, on the other hand, the bacillus subtilis strain efficiently decomposes protein combined with calcium phosphate to generate less urinary calculus, and can peel off urinary calculus which is hard and hard like stone and is difficult to decompose from facilities and convert the urinary calculus into a state which is easy to remove, so the bacillus subtilis strain can also be applied to the treatment of male urinal.
(4) According to the physicochemical property characteristics (aerobic characteristics) of the bacillus subtilis, the bacteria powder is prepared by adopting the perlite powder as the spray carrier, and the characteristics of light specific gravity and coarse particles of the perlite powder are utilized, so that the viable bacteria attached to the perlite powder are not easy to settle during biological treatment of the prepared bacteria powder, and therefore, the aerobic biochemical treatment can be performed by fully utilizing the dissolved oxygen in a septic tank or a sewage treatment tank.
(5) The bacillus subtilis preparation is simple and convenient to use, and can be used by putting bacillus subtilis QS1 microbial inoculum into the 1 st grid of a septic tank every two weeks at first without carrying out bacterial liquid inoculation treatment or operating in steps.
Drawings
FIG. 1 is a photograph comparing a first grid and a water outlet grid of a septic tank after using the preparation of the invention in a three-span septic tank in a village on a railway;
FIG. 2 is a graph showing the change of each index after a septic tank of a commercial facility in Tokyo is treated for 2 months;
FIG. 3 is a picture of a first stage of treatment of a washing plant facility with the microbial inoculum of the invention;
FIG. 4 is a picture of the second stage of a washing plant facility treated with the microbial inoculum of the invention.
Detailed Description
The invention is further described with reference to specific examples.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Example 1
This example provides a method for screening and isolating a bacillus subtilis strain according to the invention, the screening method comprising:
1) Collecting a soil sample in the inner Mongolia Hulunebergen, and extracting flora in the soil sample;
2) Culturing a soil flora in a Beijing laboratory, and confirming that the soil flora has better degradation effect on ammonia nitrogen, nitrite, COD (chemical oxygen demand) and the like in soil and sewage;
3) Screening out main bacilli in the soil flora, and confirming the excellent treatment effect on soil and sewage again;
4) The bacillus is identified, and the result proves that the bacillus is bacillus subtilis and is classified as follows: bacillus Subtilis, strain QS1. The strain identification mode is as follows: extracting genome DNA, performing molecular identification, and comprehensively analyzing according to experimental data such as cell morphology, physiological and biochemical characteristics, 16S rRNA gene sequence, phes gene sequence and the like of strains by referring to Bergerian system bacteriology handbook, and finally identifying the strains as Bacillus Subtilis.
The strain is preserved in China general microbiological culture Collection center (CGMCC); the preservation number is: CGMCC No.17314; the address of the depository: the postcode of the microbiological research institute of Chinese academy of sciences, in the sunny region of Beijing: 100101; the preservation date is as follows: year 2019, month 3, day 08, classification: bacillus Subtilis.
The bacillus subtilis CGMCC No.17314 has the character characteristics that: the strain cells are long rod-shaped, and spores are oval; the colony surface is rough and opaque, is in a dirty white color, can form wrinkle when cultured in a liquid, and can survive in a meta-acid or meta-alkali environment. The physiological and biochemical properties of the bacillus subtilis CGMCC17314 are shown in Table 1:
TABLE 1 physiological and biochemical properties of Bacillus subtilis CGMCC17314
Example 2
The method for preparing the microbial inoculum by using the bacillus subtilis strain comprises the following steps:
1) Taking a small amount of pure bacteria from a slope of preserved bacillus subtilis QS1, and inoculating the pure bacteria to a flat plate for activation;
2) Carrying out fermentation experiments by using small-scale equipment, and carrying out shake culture on an LB (LB) culture medium at 37 ℃ for 12 hours to complete fermentation; the fermentation test of the embodiment is basically consistent with the fermentation conditions of the bacillus subtilis in the prior art, and the variety and the culture conditions of the culture medium can be adjusted according to the test in the specific experimental process;
3) Spraying the fermented bacteria liquid in a spray tower, wherein the spray carrier is natural perlite powder with the particle size of about 10 mu m;
4) Directly mixing the sprayed bacterium powder with maltodextrin, and subpackaging into 30 g/bag of microbial inoculum containing Bacillus subtilis with effective viable count of 1.0 × 10 8 CFU/g。
Aiming at the treatment of septic tanks, the using mode of the bacillus subtilis QS1 microbial inoculum provided by the invention is as follows: firstly, putting bacillus subtilis QS1 bags in the 1 st grid of the septic tank every two weeks (putting one bag in each cubic meter of the volume of the septic tank); but in case of serious dirt, the dirt can be thrown into the second grid or even the third grid at the same time; the device can also be put into a kitchen sewer or a drain pipe, and the use mode can more effectively prevent the pipeline from being blocked.
When the device is used, the viable bacteria attached to the perlite powder are not easy to sink, can be diffused to the upper water body of the septic tank to treat more parts of the manure residues and pollutants, can fully utilize the dissolved oxygen in the septic tank or the sewage treatment tank to carry out aerobic biochemical treatment, the odor of the septic tank is cleared up in the first two months, the manure residues begin to be reduced, and the effluent water quality begins to be obviously improved.
Bacillus subtilis QS1 microbial inoculum is used for treating residential quarters, public toilets and rural septic tanks, and the pollutants are improved after two months of using the microbial inoculum for the public toilets and the septic tanks with different volumes shown in tables 2 to 4.
TABLE 2 pollutant treatment before and after use of one-village and three-span septic tanks in railway
Fig. 1 is a photograph comparing a first septic tank grid and a water outlet grid after a formulation of the invention is used in a three-septic tank in a single village of railway, wherein a is a photograph of the first septic tank grid, and b is a photograph of the water outlet grid of the septic tank. According to comparison, the microbial inoculum can obviously improve the effluent quality of the septic tank.
Table 3 pollutant treatment before and after use of Yuanjia post flower public latrine
TABLE 4 pollutant treatment before and after use of septic tanks in community in cross street
As can be seen from the data in tables 2 to 4, the microbial inoculum of the invention has remarkable effect on removing COD, suspended solids and total phosphorus of the septic tank.
A large number of studies show that the bacillus subtilis QS1 has good decomposition capacity aiming at urine protein, on one hand, the bacterial exoenzyme generates alkaline protease to decompose urinary calculus, on the other hand, the protein combined with calcium phosphate is efficiently decomposed, so that the urinary calculus is less generated, the urinary calculus which is hard and hard like stones and is difficult to decompose can be peeled off from facilities and converted into a state which is easy to remove, and the bacillus subtilis QS1 microbial inoculum is applied to a male urinal and forms less urinary calculus after being used for one month.
Example 3
The method for preparing the microbial inoculum by using the bacillus subtilis strain comprises the following steps:
1) Taking a small amount of pure bacteria from a slope of preserved bacillus subtilis QS1, and inoculating the pure bacteria to a flat plate for activation;
2) Carrying out fermentation experiments by using small-scale equipment, and carrying out shake culture on an LB culture medium at 37 ℃ for 24 hours to complete fermentation; the fermentation test of the embodiment is basically consistent with the fermentation conditions of the bacillus subtilis in the prior art, and the variety and the culture conditions of the culture medium can be adjusted according to the test in the specific experimental process;
3) Spraying the fermented bacteria liquid in a spray tower, wherein the spray carrier is 100 μm natural perlite powder;
4) Mixing the sprayed bacterial powder with maltodextrin and the composite biological enzyme freeze-dried powder in sequence, and subpackaging into 30 g/bag of microbial inoculum, wherein the microbial inoculum contains the effective viable count of the bacillus subtilis strain of 1.0 multiplied by 10 7 CFU/g。
The composite biological enzyme freeze-dried powder is added to activate the bacillus subtilis by using biological enzyme in the composite biological enzyme freeze-dried powder, and the preparation method comprises the following steps: a) Carrying out solid-liquid separation on the pig manure to prepare a pig urine stock solution; b) Putting the pig urine stock solution into reaction barrels, and sequentially treating according to an A/A/O process, wherein micro-nano aeration devices are respectively arranged in the first three reaction barrels; c) Repeating the A/A/O process for at least 3 times, feeding into a precipitation filter vat, and filtering to obtain compound biological enzyme with microorganism content less than 1%.
The Bacillus subtilis QS1 microbial inoculum prepared in the embodiment is applied to septic tanks of commercial facilities in Tokyoto, the change conditions of BOD, SS, NH and the like in water bodies when the bacterial inoculum is not used and after the bacterial inoculum is used for 2 months are respectively detected, and H is collected from the air in a water tank 2 S carries out front-back comparison statisticsTable 5 shows water quality improvement data of septic tanks of commercial facilities in tokyo. Fig. 2 is a graph showing changes in various indexes after a septic tank of a commercial facility in tokyo is treated for 2 months.
TABLE 5 Water quality improvement data for septic tanks in commercial facilities in Tokyo
As can be seen from Table 5, the BOD removal rate of the wastewater was 53.2%, the SS removal rate was 40.7%, and NH after 2 months 3 Removal rate of-N was 86.5%, H 2 The S removal rate is 99.3%, and the data confirm that the Bacillus subtilis QS1 microbial inoculum can effectively reduce scum and oil, can inhibit the generation of hydrogen sulfide by 99.3%, and has efficient inhibition effect on the generation of odor.
Example 4
The problem of blockage of wastewater facilities of washing plants can be solved by adopting a bacillus subtilis QS1 microbial inoculum, and the preparation method of the microbial inoculum comprises the following steps:
1) Taking a small amount of pure bacteria from a preserved Bacillus subtilis QS1 inclined plane, and inoculating the pure bacteria to a flat plate for activation;
2) Carrying out fermentation experiments by using small-scale equipment, and carrying out shake culture on an LB culture medium for 20 hours at 37 ℃ to complete fermentation; the fermentation test of the embodiment is basically consistent with the fermentation conditions of the bacillus subtilis in the prior art, and the variety and the culture conditions of the culture medium can be adjusted according to the test in the specific experimental process;
3) Spraying and drying the fermented bacteria liquid in a spray tower, wherein the spray carrier is natural perlite powder with the thickness of about 2mm to obtain bacteria powder;
4) Mixing the bacterial powder with maltodextrin, and subpackaging into 30 g/bag of microbial inoculum, wherein the microbial inoculum contains the effective viable count of bacillus subtilis strain of 1.0 multiplied by 10 10 CFU/g。
The wastewater treatment facility of the washing plant is 125m 3 Treatment tank, water discharge 100m 3/ The pH value is 9-10, the water temperature is 30-40 ℃, and the sludge in the wastewater treatment facility is blocked due to the characteristics of much oil stain and fiber in the wastewater treatment facility of a washing factory.
The wastewater treatment facilities are treated by using a bacillus subtilis QS1 microbial inoculum, fig. 3 is a picture of a first stage of washing plant facility treatment by using the microbial inoculum of the invention, and fig. 4 is a picture of a second stage of washing plant facility treatment by using the microbial inoculum of the invention.
As can be seen from the figures 3 and 4, a large amount of mud blocks and cellulose substances are decomposed into small blocks from large blocks and further decomposed, and the excrement of the septic tank contains a large amount of cellulose substances, oil stains and other ingredients, which is a main reason that the complete and effective treatment (only periodical cleaning) is difficult in the prior art, the tests prove that the bacillus subtilis QS1 has strong capacity of decomposing the substances such as the oil stains, the oil residues, the cellulose and the mud blocks, and the like. The changes of wastewater indexes of washing plants after 2 months of treatment by using the fungicide QS1 of the invention are shown in Table 6.
TABLE 6 index Change before and after wastewater treatment in washing plant
The present invention and its embodiments have been described above schematically, without limitation, and what is shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. Therefore, if the person skilled in the art receives the teaching, without departing from the spirit of the invention, the person skilled in the art shall not inventively design the similar structural modes and embodiments to the technical solution, but shall fall within the scope of the invention.
Claims (10)
1. A bacillus subtilis strain characterized by: the strain isBacillus subtilisQS1, which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms with the preservation number of CDMCCNO.17314.
2. The use of the bacillus subtilis of claim 1 in the field of septic tank treatment and sewage treatment.
3. A bacillus subtilis preparation, which is characterized in that: the Bacillus subtilis preparation comprises the Bacillus subtilis strain of claim 1.
4. The Bacillus subtilis formulation of claim 3, wherein: the method is characterized in that: the bacillus subtilis preparation also comprises natural perlite powder and auxiliary materials.
5. The Bacillus subtilis formulation of claim 4, wherein: the auxiliary material comprises maltodextrin.
6. The Bacillus subtilis formulation of claim 5, wherein: the auxiliary material also comprises composite biological enzyme freeze-dried powder.
7. A Bacillus subtilis formulation according to claim 4 or 5, wherein: the effective viable count of the bacillus subtilis preparation is 1.0 multiplied by 10 7 ~1.0×10 10 CFU/g。
8. A Bacillus subtilis formulation according to claim 4 or 5, wherein: the particle size of the natural perlite powder is 10 mu m-2 mm.
9. The method for producing a Bacillus subtilis preparation according to any one of claims 3 to 8, wherein: the method comprises the following steps:
1) Taking a small amount of pure bacteria from the preserved bacillus subtilis inclined plane, and inoculating the pure bacteria to a flat plate for activation;
2) Carrying out fermentation experiments by using small-scale equipment to obtain a bacterial liquid;
3) Spray drying the fermented bacteria liquid to obtain bacteria powder, wherein the spray carrier is natural perlite powder;
4) Mixing the obtained bacterial powder with adjuvants.
10. The use of the Bacillus subtilis preparation according to any one of claims 3 to 8 in the fields of septic tank treatment and sewage treatment.
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| CN111170571B (en) * | 2020-01-15 | 2022-04-12 | 浙江亲水园生物科技有限公司 | Liquid manure treatment process for livestock manure |
| CN111268855A (en) * | 2020-01-15 | 2020-06-12 | 浙江亲水园生物科技有限公司 | Liquid fertilization treatment method for pig farm urine-soaked manure wastewater |
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| CN114455784A (en) * | 2022-03-11 | 2022-05-10 | 洛阳博鑫生物工程科技有限公司 | Online direct drainage treatment method and treatment equipment for fecal sewage in vehicle-mounted toilet of high-speed rail motor train unit |
| CN116426402A (en) * | 2022-11-01 | 2023-07-14 | 广东省农业科学院农业生物基因研究中心 | Bacillus subtilis and application thereof |
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