CN110342651A - A kind of microbial enzyme compound formulation and preparation method thereof and its application in processing trade effluent or landfill leachate - Google Patents
A kind of microbial enzyme compound formulation and preparation method thereof and its application in processing trade effluent or landfill leachate Download PDFInfo
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- CN110342651A CN110342651A CN201910742445.XA CN201910742445A CN110342651A CN 110342651 A CN110342651 A CN 110342651A CN 201910742445 A CN201910742445 A CN 201910742445A CN 110342651 A CN110342651 A CN 110342651A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The present invention provides a kind of microbial enzyme compound formulation and preparation method thereof and its application in processing trade effluent or landfill leachate, it is related to technical field of sewage, microbial enzyme compound formulation of the present invention, including EM bacteria agent, aerobic mycoproteinase and anaerobism mycoproteinase.The present invention from the aerobic mycoproteinase and anaerobism mycoproteinase extracted in activated sludge can in EM bacteria agent aerobic microbiological and anaerobe a large amount of organized enzyme is provided, improve its initial reaction rate, and then the initial COD removal rate of EM bacteria agent can be significantly improved, the Sewage Environment that EM bacteria agent balance will likely quickly be broken is improved, thus microbial enzyme compound formulation provided by the invention can be adapted for the processing of various trade effluents or landfill leachate.
Description
Technical field
The present invention relates to technical field of sewage more particularly to a kind of microbial enzyme compound formulation and preparation method thereof and
Its application in processing trade effluent or landfill leachate.
Background technique
With the development of industrialization with the increase of urban population, urbanization constantly expands so that water and useless, dirt are used in city
Water discharge amount is continuously increased, and exacerbates short and water body the pollution of water resource, city domestic sewage and trade effluent or rubbish
Percolate improvement has become the focus being concerned in environmental protection.In recent decades, the processing technique of municipal sewage is continuous
Development, based on aerobic microbiological processing technique, is broadly divided into activated sludge process and biofilm two major classes treatment process.It is conventional
Nitrogen, the phosphorus treatment effect of activated sludge process are bad, are not easy to reach discharge standard, thus when sewage treatment by Aerobic Process for Treatment with detest
Oxygen processing combines, and further removes ammonia nitrogen.
EM (effective microorganisms) technology is university, Ryukyu, early 1980s Japan than praising according to husband
What professor et al. proposed, EM bacteria agent is one kind centered on photosynthetic bacteria, and nitrogen-fixing bacteria and deposits, breeds, using special fermentation
Technique, the composite microbial that the aerobic and anerobe that carefully screen are mixed in appropriate proportions and are turned out
Object microbial inoculum.The utility and its secretory substance that various microorganisms generate in its growth course become micropopulation growth
Matrix and raw material one complexity formd and stable microflora, hair by mutual this symbiosis Relationship With Proliferation
Wave multiple functions.Currently, EM bacteria agent agricultural, animal husbandry, aquaculture, in terms of achieve good application effect
Fruit.
After applying EM bacteria agent in sewage, beneficial bacterium can be made to occupy superiority quickly, absorb the nutrients in waste water
Matter (most of organic matter and part inorganic matter), so that sewage becomes clear, stench is eliminated, ecological environment is restored.The world
On there are many country using EM bacteria agent carry out sewage treatment, obtain relatively broad application has Japanese, Dutch, new west
Blue, Pakistan, China and Thailand etc..Sewage boiling water is handled using EM bacteria agent, effluent quality is good, and biofacies increases, and sludge produces
Amount is few, may also suppress the generation of dross.
Brave wait is opened to be studied application of the EM technology in the processing of yellow ginger processing waste water, the results showed that, EM flora can
The waste water containing high salt, grease and cyclic compound is handled, (Zhang Yong is opened and kept the characteristic with very strong resisting high-concentration sulfate
Really, Qi Encheng waits application [J] the Environmental science and technology of .EM technology in the processing of yellow ginger processing waste water, 2005 (S1): 119-
120.).Liu Jiandang and Song Fengmin etc. has studied EM bacterium and aerobic sludge Combined Treatment diosgenin wastewater, the results showed that, most
Under good experimental condition, EM bacterium and aerobic activated sludge in sbr reactor device to wastewater COD removal rate up to 97.95%, coloration is gone
It, can be useless in the synthesis of production of saponin except rate has stronger adaptability to high COD load, high salt concentration up to 66.67%, EM bacterium
(research [J] environmental pollution of Song Fengmin, Hu Shibin, Liu Yin saccharomycete processing diosgenin wastewater is controlled for growth well in water
Reason technology and equipment, 2004,5 (4): 66-69.；Ling Shi, Feng Guiying, Liu Jiandang wait Yeast-photosynthetic Bacteria to handle
The northwest experimental study [J] the agricultural journal of saponin waste water, 2006,15 (1): 109-112).Sewage treatment is carried out using EM bacteria agent
Method it is easy to operate, at low cost and good to the large amount of organic removal effect in sewage, be a kind of safely and effectively sewage
Processing method.
However, it is necessary to which the Sewage Environment of processing is different, some sewage are not suitable for the flora growth or suppressing portion of EM bacteria agent
Divide beneficial microorganism growth, such as photosynthetic bacteria etc..The functional flora for being not suitable for will lead to EM bacteria agent of Sewage Environment is unbalance,
Break the symbiosis Relationship With Proliferation of EM flora, so that EM bacteria agent can not play effective purification sewage effect.Therefore, this field needs
Solve the problems, such as that EM bacteria agent can not deliver directly use.
Summary of the invention
The present invention in order to overcome existing EM bacteria agent can not adapt to sewage treatment caused by part of contaminated water environment failure lack
It falls into, provides a kind of microbial enzyme compound formulation comprising EM bacteria agent, the initial purge rate of EM bacteria agent can be effectively improved, fastly
Sewage is transformed into the environment of suitable EM bacteria agent existence by speed.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of microbial enzyme compound formulations, including EM bacteria agent, aerobic mycoproteinase and anaerobism mycoprotein
Enzyme.
Preferably, the mass ratio of the EM bacteria agent, aerobic mycoproteinase and anaerobism mycoproteinase be 5~10: 2~5: 3~
7。
Preferably, the dosage form of the microbial compound preparation includes pulvis, granule, liquid preparation, tablets and capsules.
The present invention also provides the preparation methods of microbial enzyme compound formulation described in above-mentioned technical proposal, including following step
It is rapid:
(1) the aerobic tank pool bottom sludge and anaerobic pond pool bottom sludge of sewage treatment plant are taken, after diluting 5~10 times respectively with water
Filtering, obtains aerobic coarse filtration liquid and anaerobism coarse filtration liquid；
(2) by aerobic coarse filtration liquid and anaerobism coarse filtration liquid respectively cross 0.45 μm of filtering with microporous membrane, obtain aerobic refined filtration liquid and
Anaerobism refined filtration liquid；
(3) aerobic refined filtration liquid is accessed into 3~7d of aerobic culture in fermentation medium, obtains aerobic fermentation liquid；
Anaerobism refined filtration liquid is accessed into 3~7d of Anaerobic culturel in fermentation medium, obtains anaerobic fermented liquid；
(4) aerobic fermentation liquid and anaerobic fermented liquid are centrifuged respectively, the precipitating of centrifugation is taken to carry out clasmatosis, alkali carries respectively
Acid is heavy to extract albumen, obtains aerobic mycoproteinase and anaerobism mycoproteinase；
(5) EM bacteria agent, aerobic mycoproteinase and anaerobism mycoproteinase are mixed in proportion to get the compound system of microbial enzyme
Agent.
Preferably, the filter sizes of step (1) described filtering are 1~50 μm.
Preferably, step (3) fermentation medium is trade effluent or landfill leachate.
Preferably, the method for step (4) described clasmatosis is ultrasonication.
The present invention also provides microbial enzyme compound formulations described in preceding solution to seep in processing trade effluent or rubbish
Application in filtrate.
Preferably, the aerobic mycoproteinase in the microbial enzyme compound formulation and anaerobism mycoproteinase are by be processed
What trade effluent or landfill leachate were obtained as fermentation medium.
Preferably, the microbial enzyme compound formulation is according to viable count 0.5 × 104~4 × 104A every cubic metre of industry is dirty
The ratio of water or landfill leachate is added in trade effluent or landfill leachate.
Compared with prior art, beneficial effects of the present invention:
The present invention provides a kind of microbial enzyme compound formulations, including EM bacteria agent, aerobic mycoproteinase and anaerobism mycoprotein
Enzyme.The present invention can be aerobic micro- in EM bacteria agent from the aerobic mycoproteinase and anaerobism mycoproteinase extracted in activated sludge
Biology and anaerobe provide a large amount of organized enzyme, improve its initial reaction rate, and then can significantly improve EM bacteria agent
Initial COD removal rate, the Sewage Environment that will likely quickly break EM bacteria agent balance are improved, thus provided by the invention micro-
Biological enzyme compound formulation can be adapted for the processing of various trade effluents or landfill leachate.
The present invention also provides microbial enzyme compound formulations described in above-mentioned technical proposal to seep in processing trade effluent or rubbish
Microbial enzyme compound formulation of the present invention is direct plungeed into trade effluent or landfill leachate to be processed by the application in filtrate
, easy to operate, at low cost, good sewage processing effect.
Specific embodiment
The present invention provides a kind of microbial enzyme compound formulations, including EM bacteria agent, aerobic mycoproteinase and anaerobism mycoprotein
Enzyme.In the present invention, the mass ratio of the EM bacteria agent, aerobic mycoproteinase and anaerobism mycoproteinase is preferably 5~10: 2~5: 3
~7；More preferably 6~8: 3~4: 4~6.Aerobic mycoproteinase and anaerobism mycoproteinase of the present invention are complex enzyme,
From the aerobic tank pool bottom sludge and anaerobic pond pool bottom sludge of sewage treatment plant.
In the present invention, the living bacteria count in the microbial enzyme compound formulation is preferably not less than 1 × 105It is a, more
Preferably 1 × 105~1 × 106It is a.In the present invention, the dosage form of the microbial compound preparation include but is not limited to pulvis,
Granula, liquid preparation, tablets and capsules.In the present invention, the microbial compound preparation can also include auxiliary material, described auxiliary
Material includes but is not limited to filler, stabilizer, preservative, pH adjusting agent and microbial nutrition substance.
The present invention also provides the preparation methods of microbial enzyme compound formulation described in above-mentioned technical proposal, including following step
It is rapid:
(1) the aerobic tank pool bottom sludge and anaerobic pond pool bottom sludge of sewage treatment plant are taken, after diluting 5~10 times respectively with water
Filtering, obtains aerobic coarse filtration liquid and anaerobism coarse filtration liquid；
(2) by aerobic coarse filtration liquid and anaerobism coarse filtration liquid respectively cross 0.45 μm of filtering with microporous membrane, obtain aerobic refined filtration liquid and
Anaerobism refined filtration liquid；
(3) aerobic refined filtration liquid is accessed into 3~7d of aerobic culture in fermentation medium, obtains aerobic fermentation liquid；
Anaerobism refined filtration liquid is accessed into 3~7d of Anaerobic culturel in fermentation medium, obtains anaerobic fermented liquid；
(4) aerobic fermentation liquid and anaerobic fermented liquid are centrifuged respectively, the precipitating of centrifugation is taken to carry out clasmatosis, alkali carries respectively
Acid is heavy to extract albumen, obtains aerobic mycoproteinase and anaerobism mycoproteinase；
(5) EM bacteria agent, aerobic mycoproteinase and anaerobism mycoproteinase are mixed in proportion to get the compound system of microbial enzyme
Agent.
The present invention takes the aerobic tank pool bottom sludge and anaerobic pond pool bottom sludge of sewage treatment plant, respectively with water dilution 5~10
Filtering, obtains aerobic coarse filtration liquid and anaerobism coarse filtration liquid after times.The purpose that the present invention is diluted and filtered with water be in order to by sludge with
Microorganism therein carries out initial gross separation, removes the body refuse of bulk.In the present invention, the extension rate of the water refers to bottom of pond dirt
The ratio between volume and the volume of water of mud, the water extension rate are preferably 6~8 times.In the present invention, the filter hole of the filtering
Diameter is preferably 1~50 μm.In the present invention, the method for the filtering includes filtering.
After obtaining aerobic coarse filtration liquid and anaerobism coarse filtration liquid, aerobic coarse filtration liquid and anaerobism coarse filtration liquid are crossed 0.45 by the present invention respectively
μm filtering with microporous membrane, obtains aerobic refined filtration liquid and anaerobism refined filtration liquid.What the present invention was filtered using 0.45 μm of miillpore filter
Purpose is further to be separated the microorganisms such as bacterium in coarse filtration liquid with other impurities.In the present invention, 0.45 μ
The number of m filtering with microporous membrane is preferably 2~3 times, and when multiple 0.45 μm of filtering with microporous membrane merges filtrate.
After obtaining aerobic refined filtration liquid and anaerobism refined filtration liquid, aerobic refined filtration liquid is accessed aerobic training in fermentation medium by the present invention
3~7d is supported, aerobic fermentation liquid is obtained；Anaerobism refined filtration liquid is accessed into 3~7d of Anaerobic culturel in fermentation medium, obtains anaerobic fermentation
Liquid.The present invention is to be enriched with wherein with the purpose that fermentation medium respectively ferments to aerobic refined filtration liquid and anaerobism refined filtration liquid
Aerobic bacteria and anaerobic bacteria.In the present invention, the temperature of the aerobic culture is preferably 25~35 DEG C, and more preferably 28 DEG C.?
In the present invention, the time of the aerobic culture is preferably 4~6d, more preferably 5d.In the present invention, the temperature of the Anaerobic culturel
Preferably 25~35 DEG C, more preferably 28 DEG C of degree.In the present invention, the time of the Anaerobic culturel is preferably 4~6d, more excellent
It is selected as 5d.In the present invention, the fermentation medium is preferably trade effluent or landfill leachate, utilizes trade effluent or rubbish
On the one hand percolate can save toxigenic capacity as fermentation medium, on the other hand aerobic bacteria or anaerobic bacteria can be made to secret out of more
It is suitable for the enzyme for decomposing trade effluent or landfill leachate, preferably to assist EM bacteria agent to carry out sewage treatment more.
After obtaining aerobic fermentation liquid and anaerobic fermented liquid, the present invention is respectively centrifuged aerobic fermentation liquid and anaerobic fermented liquid,
It takes the precipitating of centrifugation to carry out clasmatosis, the heavy extraction albumen of alkali carries acid respectively, obtains aerobic mycoproteinase and anaerobism mycoproteinase.
In the present invention, the rate of the centrifugation is preferably 1500~3000rpm, more preferably 2000rpm；The time of the centrifugation is excellent
It is selected as 3~5min.In the present invention, the method for the clasmatosis is preferably ultrasonication, work of the ultrasonication to protease
Property destroy it is low.In the present invention, preferably precipitating is mixed with physiological saline according to volume ratio 1: 1~5 before the ultrasonication,
To complete ultrasound.The specific nature of acid and base sites that the present invention sinks to the alkali carries acid is not particularly limited, and can complete protein extraction i.e.
It can.
After obtaining aerobic mycoproteinase and anaerobism mycoproteinase, the present invention is by EM bacteria agent, aerobic mycoproteinase and anaerobic bacteria
Protease is mixed in proportion to get microbial enzyme compound formulation.If microbial enzyme compound formulation of the present invention further includes auxiliary material,
EM bacteria agent, the mixture of aerobic mycoproteinase and anaerobism mycoproteinase and auxiliary material are then mixed and made into different dosage forms again, this
Invention is not particularly limited this.
The present invention also provides microbial enzyme compound formulations described in preceding solution to seep in processing trade effluent or rubbish
Application in filtrate.It is currently preferred, aerobic mycoproteinase and anaerobism mycoproteinase in the microbial enzyme compound formulation
It is to be obtained by trade effluent or landfill leachate to be processed as fermentation medium.The present invention can targetedly treat place
The trade effluent or landfill leachate of reason customize special microbial enzyme compound formulation, better processing effect.
In the present invention, the microbial enzyme compound formulation is preferably according to viable count 0.5 × 104~4 × 104It is a often to stand
The ratio of square rice trade effluent or landfill leachate is added in trade effluent or landfill leachate, and viable count is more preferably 1 × 104
~2 × 104A every cubic metre of trade effluent or landfill leachate.Microbial compound preparation of the present invention is applicable to various next
The trade effluent or landfill leachate treatment in source are directly applied, easy to use.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
Voluntarily prepare CODCrConcentration is 3000mg/L, BOD5Concentration is the synthetic wastewater of 1200mg/L, pH value 10~11.
(1) the aerobic tank pool bottom sludge and each 10L of anaerobic pond pool bottom sludge of Shanghai somewhere sewage treatment plant are taken, respectively
It is added after the water mixing of 10 times of volumes with 30 μm of membrane filtrations, obtains aerobic coarse filtration liquid and anaerobism coarse filtration liquid；
(2) by aerobic coarse filtration liquid and anaerobism coarse filtration liquid respectively cross 0.45 μm of filtering with microporous membrane, obtain aerobic refined filtration liquid and
Anaerobism refined filtration liquid；
(3) aerobic refined filtration liquid is accessed into aerobic culture 5d in synthetic wastewater, obtains aerobic fermentation liquid；Anaerobism refined filtration liquid is connect
Enter Anaerobic culturel 7d in synthetic wastewater, obtains anaerobic fermented liquid；
(4) aerobic fermentation liquid and anaerobic fermented liquid centrifugation (centrifugal rotational speed 2500rpm is centrifuged 3min) are taken respectively respectively
The precipitating of centrifugation according to ultrasonic cell disintegration is carried out after volume ratio 1: 1 plus physiological saline, obtain broken aerobic fermentation liquid and
Broken anaerobic fermented liquid.Add 0.1M's into broken aerobic fermentation liquid and broken anaerobic fermented liquid respectively
NaOH solution to pH value is 12.0, stands 2h, and HCl solution to the pH value for adding 0.1M is 3.5, is filtered after standing 2h again,
Precipitating is aerobic mycoproteinase and anaerobism mycoproteinase；
(5) by EM bacteria agent (the EM bacteria agent stoste of commercially available purchase), aerobic mycoproteinase and anaerobism mycoproteinase in mass ratio 6
: 4: 4 ratio mixes to get microbial enzyme compound formulation.
Embodiment 2
Voluntarily prepare CODCrConcentration is 3000mg/L, BOD5Concentration is the synthetic wastewater of 1200mg/L, pH value 10~11.
(1) the aerobic tank pool bottom sludge and each 10L of anaerobic pond pool bottom sludge of Shanghai somewhere sewage treatment plant are taken, respectively
It is added after the water mixing of 5 times of volumes with 5 μm of membrane filtrations, obtains aerobic coarse filtration liquid and anaerobism coarse filtration liquid；
(2) by aerobic coarse filtration liquid and anaerobism coarse filtration liquid respectively cross 0.45 μm of filtering with microporous membrane, obtain aerobic refined filtration liquid and
Anaerobism refined filtration liquid；
(3) aerobic refined filtration liquid is accessed into aerobic culture 7d in synthetic wastewater, obtains aerobic fermentation liquid；Anaerobism refined filtration liquid is connect
Enter Anaerobic culturel 7d in synthetic wastewater, obtains anaerobic fermented liquid；
(4) aerobic fermentation liquid and anaerobic fermented liquid centrifugation (centrifugal rotational speed 1500rpm is centrifuged 4min) are taken respectively respectively
The precipitating of centrifugation according to ultrasonic cell disintegration is carried out after volume ratio 1: 2 plus physiological saline, obtain broken aerobic fermentation liquid and
Broken anaerobic fermented liquid.Add 0.1M's into broken aerobic fermentation liquid and broken anaerobic fermented liquid respectively
NaOH solution to pH value is 12.0, stands 2h, and HCl solution to the pH value for adding 0.1M is 3.5, is filtered after standing 2h again,
Precipitating is aerobic mycoproteinase and anaerobism mycoproteinase；
(5) by EM bacteria agent (the EM bacteria agent stoste of commercially available purchase), aerobic mycoproteinase and anaerobism mycoproteinase in mass ratio 7
: 3: 6 ratio mixes to get microbial enzyme compound formulation.
Embodiment 3
Voluntarily prepare CODCrConcentration is 3000mg/L, BOD5Concentration is the synthetic wastewater of 1200mg/L, pH value 10~11.
The microbial enzyme compound formulation that respectively prepared by embodiment 1, EM bacteria agent are according to viable count 1 × 105A/cubic meter is dirty
In the ratio investment 10L synthetic wastewater of water, it is denoted as experimental group and control group, continuously stirs 72h respectively, every 2h blast aeration one
It is secondary.
1, it takes the experimental group of processing 2h and control group water sample to carry out microscopy, and is observed using biological micro-image analysis system
Biofacies.
Microscopic examination result shows without photosynthetic bacteria and der Pilz in control group 2h water sample, and then have in test group 2h water sample photosynthetic
Bacterium and der Pilz.Early-stage study discovery when using above-mentioned synthetic wastewater culture EM bacteria agent, will lead to losing for photosynthetic bacteria and der Pilz
It loses, this may be because homemade synthetic wastewater is unsatisfactory for the growth of certain micro-organisms population in EM bacteria agent, to break EM
The mutual Symbiotic effectiveness of flora.And the present invention is added to the microbial enzyme compound formulation of aerobic mycoproteinase and anaerobism mycoproteinase
Flora is realized in identical synthetic wastewater not lose, overcome EM bacteria agent in part trade effluent or landfill leachate its
The problem of colony balance is broken.
2, processing 2h is taken respectively, for 24 hours with the experimental group of 72h and control group water sample, measure each water sample using potassium dichromate method
In CODCrConcentration, and calculate the COD at different disposal momentCrRemoval rate, the results are shown in Table 1.
The COD of 1 different disposal time of tableCrRemoval rate
| Group | Handle the COD of 2hCrRemoval rate | The COD of processing for 24 hoursCrRemoval rate | Handle the COD of 72hCrRemoval rate |
| Experimental group | 23.7% | 58.1% | 82.2% |
| Control group | 10.2% | 53.0% | 76.8% |
It can be seen from the data of table 1 compared with the control group, COD of the experimental group in 2hCrRemoval rate is significantly increased, table
Bright microbial enzyme compound formulation provided by the invention can effectively improve purification efficiency when initial treatment, quickly improve dirt to be processed
The environment of water, makes EM bacteria agent be more easily adapted to conform to Sewage Environment.Experimental group processing for 24 hours, the COD of 72hCrRemoval rate is all remarkably higher than pair
According to group, show that microbial compound preparation provided by the invention is more excellent to the effect of sewage treatment.
Embodiment 4
Take Shanghai City somewhere CODCrConcentration is 7000~9000mg/L, BOD5Concentration is 4500~6000mg/L, pH value 5
~6 landfill leachate.It is handled for 24 hours with the microbial enzyme compound formulation that embodiment 1 is prepared, the rubbish after detection processing seeps
COD in filtrateCrConcentration, the results show that the COD after for 24 hoursCrRemoval rate is 51.6%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of microbial enzyme compound formulation, which is characterized in that including EM bacteria agent, aerobic mycoproteinase and anaerobism mycoproteinase.
2. microbial enzyme compound formulation according to claim 1, which is characterized in that the EM bacteria agent, aerobic mycoproteinase
Mass ratio with anaerobism mycoproteinase is 5~10: 2~5: 3~7.
3. microbial compound preparation according to claim 1 or 2, which is characterized in that the agent of the microbial compound preparation
Type includes pulvis, granule, liquid preparation, tablets and capsules.
4. the preparation method of microbial enzyme compound formulation described in claims 1 to 3 any one, which is characterized in that including following
Step:
(1) the aerobic tank pool bottom sludge and anaerobic pond pool bottom sludge of sewage treatment plant are taken, mistake after diluting 5~10 times respectively with water
Filter, obtains aerobic coarse filtration liquid and anaerobism coarse filtration liquid；
(2) by the aerobic coarse filtration liquid and anaerobism coarse filtration liquid respectively cross 0.45 μm of filtering with microporous membrane, obtain aerobic refined filtration liquid and
Anaerobism refined filtration liquid；
(3) by 3~7d of aerobic culture in the aerobic refined filtration liquid access fermentation medium, aerobic fermentation liquid is obtained；
The anaerobism refined filtration liquid is accessed into 3~7d of Anaerobic culturel in fermentation medium, obtains anaerobic fermented liquid；
(4) the aerobic fermentation liquid and anaerobic fermented liquid are centrifuged respectively, the precipitating of centrifugation is taken to carry out clasmatosis, alkali carries respectively
Acid is heavy to extract albumen, obtains aerobic mycoproteinase and anaerobism mycoproteinase；
(5) EM bacteria agent, aerobic mycoproteinase and anaerobism mycoproteinase are mixed in proportion to get microbial enzyme compound formulation.
5. the preparation method according to claim 4, which is characterized in that the filter sizes of step (1) described filtering are 1~50
μm。
6. the preparation method according to claim 4, which is characterized in that step (3) fermentation medium is trade effluent
Or landfill leachate.
7. the preparation method according to claim 4, which is characterized in that the method for step (4) described clasmatosis is ultrasound
It is broken.
8. microbial enzyme compound formulation described in claims 1 to 3 any one is in processing trade effluent or landfill leachate
Using.
9. application according to claim 8, which is characterized in that the microbial enzyme compound formulation according to viable count 0.5 ×
104~4 × 104The ratio of a every cubic metre of trade effluent or landfill leachate is added in trade effluent or landfill leachate.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114213191A (en) * | 2021-11-23 | 2022-03-22 | 清大国杰生物科技有限公司 | Agricultural folic acid active mycoprotein polypeptidase plant enzyme, formula and preparation method |
| CN117683682A (en) * | 2023-12-13 | 2024-03-12 | 江苏省扬州环境监测中心 | Microbial composition for degrading straw |
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| CN117683682A (en) * | 2023-12-13 | 2024-03-12 | 江苏省扬州环境监测中心 | Microbial composition for degrading straw |
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