CN110420142A - A kind of extracting method of Aplotaxis auriculata extract - Google Patents
A kind of extracting method of Aplotaxis auriculata extract Download PDFInfo
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Abstract
The invention discloses a kind of extracting methods of Aplotaxis auriculata extract, it is related to cosmetics extractive technique field, it solves because of the polysaccharide compound content problem that is lower, and causing its overall efficacy lower in Aplotaxis auriculata extracting solution, and the following steps are included: step 1, stock, Aplotaxis auriculata raw material is taken, is dried at 40-60 DEG C to constant weight, is crushed, and 40-50 mesh is crossed, obtain Aplotaxis auriculata powder；Step 2, polysaccharide water extract, Aplotaxis auriculata powder are placed in container, the distilled water that 14-18 times of Aplotaxis auriculata powder is measured are added, and water-bath is extracted 2-3 times at 70-80 DEG C, each 1.5-2.5h obtains extracting solution；Step 3, concentration, extracting solution filter while hot, and filtrate water-bath is concentrated into 0.5-1ml/g crude drug；Step 4, alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, alcohol content is made to reach 70-80%, stands 22-26h in 4-6 DEG C of refrigerator, is filtered with miillpore filter, and 50-60 DEG C of precipitating dries to constant weight, and Aplotaxis auriculata extract can be obtained.In the Aplotaxis auriculata extract obtained through the invention, compound of polysaccharide content with higher.
Description
Technical field
The present invention relates to cosmetics extractive technique fields, more specifically, it relates to a kind of extraction of Aplotaxis auriculata extract
Method.
Background technique
Aplotaxis auriculata is perennial catananche, and the polysaccharide compound of radix aucklandiae has good anti-complement activity,
To the immunocompetent important function of physical exertion.Meanwhile the polysaccharide of Aplotaxis auriculata has and inhales as a kind of natural plant extracts
Wet moisturizing, anti-oxidant, anti-aging, repairing skin tissue, anti-inflammatory, antibacterial, anti-radiation, Bearberry Extract, in Essence, moisturizing
There is good application effect in the cosmetics such as agent.
A kind of shaddock leaf promoting qi circulation and relieving pain is disclosed in the Chinese invention patent application file of Publication No. CN106387152A
Tealeaves and preparation method thereof is prepared by tealeaves, Chinese herbal medicine extract by the mass ratio of 1:4, the Chinese herbal medicine extract
In, raw material by weight percentage, shaddock leaf 20-40%, oil head grass 10-20%, Gu Mu 10-15%, brassicae chinensis,semen 10-15%, Radix Curcumae
5-10%, difformed galingale herb 5-10%, Aplotaxis auriculata 5-10%, in one's early teens 3-5%, prepared pinellia rhizome 3-5%, small viscous medicine 3-5%, preparation
The key step of method are as follows: a, stock；B, filtrate is extracted；C, centrifuge separation, concentration；D, smearing, drying；E, parch, sterilizing.
In above-mentioned application documents, the raw material after cleaning and cleaning is placed in extractor, is extracted 3 times, adds raw material for the first time
The water that 12 times of weight, second plus 10 times of raw material weight of water, third time plus 8 times of raw material weight of water, extraction time be respectively
120 minutes, 90 minutes, 60 minutes, combined extract obtain the filtrate of concentration, but water is used only and is repeatedly extracted to Aplotaxis auriculata,
Cause the polysaccharide compound content of Aplotaxis auriculata in extracting solution lower, and then cause the overall efficacy of extracting solution bad, therefore, needs
The new scheme of one kind is proposed to solve the above problems.
Summary of the invention
For in the prior art because the polysaccharide compound content in Aplotaxis auriculata extracting solution is lower, and lead to its overall efficacy
Lower problem, the purpose of the present invention is to provide a kind of extracting methods of Aplotaxis auriculata extract, to solve the above technical problems,
Polysaccharide compound yield in its obtained Aplotaxis auriculata extract is higher, and then makes Aplotaxis auriculata extract function with higher
Effect.
To achieve the above object one, the present invention provides the following technical scheme that
A kind of extracting method of Aplotaxis auriculata extract, comprising the following steps:
Step 1, stock, takes Aplotaxis auriculata raw material, dries at 40-60 DEG C to constant weight, is crushed, and crosses 40-50 mesh, obtains
To Aplotaxis auriculata powder；
Step 2, polysaccharide water extract, Aplotaxis auriculata powder are placed in a container, and the distillation that 14-18 times of Aplotaxis auriculata powder is measured is added
Water, and water-bath is extracted 2-3 times at 70-80 DEG C, each 1.5-2.5h obtains extracting solution；
Step 3, concentration, extracting solution filter while hot, and obtained filtrate water-bath is concentrated into 0.5-1ml/g crude drug；
Step 4, alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, alcohol content is made to reach 70-80%, in 4-6 DEG C of refrigerator
22-26h is stood, is filtered with miillpore filter, 50-60 DEG C of precipitating dries to constant weight, and Aplotaxis auriculata extract can be obtained.
By using above-mentioned technical proposal, after Aplotaxis auriculata raw material drying is crushed, 40-50 mesh is crossed, particle can be obtained
The more uniform Aplotaxis auriculata powder of size, makes Aplotaxis auriculata during the extraction process, in polysaccharide compound can preferably analyse
Out.It is first extracted repeatedly repeatedly in hot bath with distilled water, the polysaccharide compound in Aplotaxis auriculata powder can be made to be precipitated completely,
And temperature control is able to maintain the anti-complement activity of polysaccharide compound at 70-80 DEG C.Extracting solution is filtered while hot dense
Contracting, and to 0.5-1ml/g crude drug, some impurity components in water can be made to fling to, and concentrate polysaccharide compound.Alcohol precipitation is dry
It is dry, so that alcohol content is reached 70-80%, is both able to maintain the anti-complement activity of polysaccharide compound, moreover it is possible to guarantee polysaccharide compound
Eduction rate, and then guarantee Aplotaxis auriculata extract in polysaccharide compound yield.
Further preferably, the step 1 is specifically configured to, and stock takes Aplotaxis auriculata raw material, is dried extremely at 40-60 DEG C
Constant weight is crushed, and crosses 40-50 mesh, then uses soaked in absolute ethyl alcohol 5-10min, after being centrifuged air drying, obtains Yun Mu
Face powder end.
By using above-mentioned technical proposal, the Aplotaxis auriculata raw material after pulverizing and sieving can be removed with soaked in absolute ethyl alcohol
The purity of polysaccharide compound in Aplotaxis auriculata extract not only can be improved, additionally it is possible to color be discharged in pigment in Aplotaxis auriculata raw material
Element can be improved the yield of Aplotaxis auriculata extract to the interference during subsequent extracted, and then it is good to have Aplotaxis auriculata extract
Good quality.
Further preferably, the step 4 is specifically configured to, and alcohol precipitation is dry, methanol is added in the filtrate of concentration: first
Sour (19:1), makes alcohol content reach 70-80%, stands 22-26h in 4-6 DEG C of refrigerator, filtered with miillpore filter, precipitates 50-60
It DEG C dries to constant weight, Aplotaxis auriculata extract can be obtained.
By using above-mentioned technical proposal, methanol is a kind of solvent more better than ethyl alcohol, and formic acid has good reproducibility,
And methanol and formic acid is used to mix, alcohol precipitation drying is carried out to concentrate, the anticomplement of polysaccharide compound can not only be kept living
Property, so that Aplotaxis auriculata extract is integrally kept good quality, additionally it is possible to keep the polysaccharide compound in concentrate completely fast
The sedimentation of speed, greatly improves the yield of Aplotaxis auriculata extract.
Further preferably, the step 4 is specifically configured to, and alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, makes
Alcohol content reaches 70-80%, is ultrasonically treated 20-30min, is centrifuged 5-10min, supernatant liquor is taken to stand 22- in 4-6 DEG C of refrigerator
26h is filtered with miillpore filter, is precipitated 50-60 DEG C twice and is dried to constant weight, Aplotaxis auriculata extract can be obtained.
By using above-mentioned technical proposal, after ethyl alcohol is added in concentrate, it is ultrasonically treated, alcohol can be made uniform
It is distributed in concentrate, and can quickly play part alcohol precipitation effect, the polysaccharide chemical combination that after centrifugation part can quickly obtained
Object is precipitated, and supernatant liquor is then stood 22-26h in 4-6 DEG C of refrigerator, the polysaccharide compound in concentrate can be made complete
It is precipitated, greatly improves the yield of Aplotaxis auriculata extract.
Further preferably, in the step 4, the power of ultrasonic treatment is 200-250W, frequency 30-50kHz.
Further preferably, in the step 4, the revolving speed of centrifugation is 2500-3500rpm.
Further preferably, in the step 4, the aperture of miillpore filter is 0.4-0.5 μm.
It by using above-mentioned technical proposal, is controlled, is able to maintain efficient steady in entire extraction process using above-mentioned parameter
It is fixed, and be not easy to damage the anti-complement activity of polysaccharide compound, the Aplotaxis auriculata extract enabled to has higher
Quality.
Further preferably, in the step 4, the filtrate of concentration is pre-processed, and specifically comprises the following steps:
The filtrate of concentration is placed in a container, potassium ferrocyanide solution and zinc acetate is added dropwise during being constantly stirred
Solution is filtered removal of impurities, obtains pretreated concentration filtrate until the turbidity of the filtrate of observation concentration no longer improves.
By using above-mentioned technical proposal, potassium ferrocyanide solution and acetic acid zinc solution can make the albumen of the filtrate of concentration
Matter generates precipitating, and the filtrate of concentration generates muddiness, and during dropwise addition, until the turbidity of the filtrate of observation concentration no longer mentions
Height then illustrates that protein precipitates completely, and by protein removal, the recovery rate for improving polysaccharide compound is not only contributed to,
The overall quality of Aplotaxis auriculata extract can also be improved, and then makes the extracting method of Aplotaxis auriculata extract that integrally there is good application
Effect.
In conclusion compared with prior art, the invention has the following advantages:
(1) by after the dry crushing of Aplotaxis auriculata raw material, and the more uniform Aplotaxis auriculata powder of granular size is obtained, then first uses distilled water
It is extracted repeatedly repeatedly in hot bath, then filtering and concentrating while hot is carried out to extracting solution, finally carried out alcohol precipitation drying, be both able to maintain more
The anti-complement activity of saccharide compound, moreover it is possible to guarantee the eduction rate of polysaccharide compound, and then be Aplotaxis auriculata extract have compared with
High yield；
(2) it after ethyl alcohol being added in concentrate, is ultrasonically treated, alcohol can be made to be evenly distributed in concentrate, and can be fast
Speed plays part alcohol precipitation effect, and the polysaccharide compound that after centrifugation part can quickly obtained is precipitated, then by supernatant liquor
22-26h is stood in 4-6 DEG C of refrigerator, the polysaccharide compound in concentrate can be made to be precipitated completely, makes Aplotaxis auriculata extract
Yield greatly improves；
(3) it is mixed using methanol and formic acid, alcohol precipitation drying is carried out to concentrate, the anti-benefit of polysaccharide compound can not only be kept
Body activity, makes Aplotaxis auriculata extract integrally keep good quality, additionally it is possible to keep the polysaccharide compound in concentrate complete
Complete quickly sedimentation, greatly improves the yield of Aplotaxis auriculata extract.
Detailed description of the invention
Fig. 1 is the extracting method flow chart of medium cloud Radix Aucklandiae extract of the present invention.
Specific embodiment
With reference to the accompanying drawings and examples, the present invention will be described in detail.
Embodiment 1: a kind of extracting method of Aplotaxis auriculata extract specifically comprises the following steps:
Step 1, stock, takes Aplotaxis auriculata raw material, dries at 50 DEG C to constant weight, is crushed, and crosses 45 meshes, obtains Yun Mu
Face powder end；
Step 2, polysaccharide water extract, Aplotaxis auriculata powder are placed in a container, and the distilled water of 16 times of Aplotaxis auriculata powder amounts is added,
And water-bath is extracted 2 times at 75 DEG C, each 2h obtains extracting solution；
Step 3, concentration, extracting solution filter while hot, and obtained filtrate water-bath is concentrated into 0.75ml/g crude drug；
Step 4, alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, alcohol content is made to reach 75%, stands in 5 DEG C of refrigerators
For 24 hours, it is filtered with miillpore filter, the aperture of miillpore filter is 0.45 μm, and 55 DEG C of precipitating dries to constant weight, and Aplotaxis auriculata extraction can be obtained
Object.
Embodiment 2: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, it specifically includes
Following steps:
Step 1, stock, takes Aplotaxis auriculata raw material, dries at 40 DEG C to constant weight, is crushed, and crosses 40 meshes, obtains Yun Mu
Face powder end；
Step 2, polysaccharide water extract, Aplotaxis auriculata powder are placed in a container, and the distilled water of 14 times of Aplotaxis auriculata powder amounts is added,
And water-bath is extracted 2 times at 70 DEG C, each 1.5h obtains extracting solution；
Step 3, concentration, extracting solution filter while hot, and obtained filtrate water-bath is concentrated into 0.5ml/g crude drug；
Step 4, alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, alcohol content is made to reach 70%, stands in 4 DEG C of refrigerators
22h is filtered with miillpore filter, and the aperture of miillpore filter is 0.4 μm, and 50 DEG C of precipitating dries to constant weight, and Aplotaxis auriculata extraction can be obtained
Object.
Embodiment 3: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, it specifically includes
Following steps:
Step 1, stock, takes Aplotaxis auriculata raw material, dries at 60 DEG C to constant weight, is crushed, and crosses 50 meshes, obtains Yun Mu
Face powder end；
Step 2, polysaccharide water extract, Aplotaxis auriculata powder are placed in a container, and the distilled water of 18 times of Aplotaxis auriculata powder amounts is added,
And water-bath is extracted 3 times at 80 DEG C, each 2.5h obtains extracting solution；
Step 3, concentration, extracting solution filter while hot, and obtained filtrate water-bath is concentrated into 1ml/g crude drug；
Step 4, alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, alcohol content is made to reach 80%, stands in 6 DEG C of refrigerators
26h is filtered with miillpore filter, and the aperture of miillpore filter is 0.5 μm, and 60 DEG C of precipitating dries to constant weight, and Aplotaxis auriculata extraction can be obtained
Object.
Embodiment 4: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, step 1 is specific
It is set as, stocks up, take Aplotaxis auriculata raw material, dry at 50 DEG C to constant weight, crushed, and cross 45 meshes, then with anhydrous second
Alcohol impregnates 7.5min, after being centrifuged air drying, obtains Aplotaxis auriculata powder.
Embodiment 5: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, step 1 is specific
It is set as, stocks up, take Aplotaxis auriculata raw material, dry at 50 DEG C to constant weight, crushed, and cross 45 meshes, then with anhydrous second
Alcohol impregnates 5min, after being centrifuged air drying, obtains Aplotaxis auriculata powder.
Embodiment 6: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, step 1 is specific
It is set as, stocks up, take Aplotaxis auriculata raw material, dry at 50 DEG C to constant weight, crushed, and cross 45 meshes, then with anhydrous second
Alcohol impregnates 10min, after being centrifuged air drying, obtains Aplotaxis auriculata powder.
Embodiment 7: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, step 4 is specific
It is set as, alcohol precipitation is dry, and methanol is added in the filtrate of concentration: formic acid (19:1) makes alcohol content reach 75%, in 5 DEG C of refrigerators
Middle standing for 24 hours, is filtered with miillpore filter, and 55 DEG C of precipitating dries to constant weight, and Aplotaxis auriculata extract can be obtained.
Embodiment 8: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, step 4 is specific
It is set as, alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, and alcohol content is made to reach 75%, 25min is ultrasonically treated, at ultrasound
The power of reason is 225W, frequency 30-50kHz, is centrifuged 7.5min, and the revolving speed of centrifugation is 3000rpm, takes supernatant liquor in 5 DEG C
It stands in refrigerator for 24 hours, is filtered with miillpore filter, the aperture of miillpore filter is 0.45 μm, precipitates 55 DEG C twice and dries to constant weight
Obtain Aplotaxis auriculata extract.
Embodiment 9: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, step 4 is specific
It is set as, alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, and alcohol content is made to reach 75%, 20min is ultrasonically treated, at ultrasound
The power of reason is 250W, frequency 50kHz, is centrifuged 5min, and the revolving speed of centrifugation is 3500rpm, takes supernatant liquor in 5 DEG C of refrigerators
It stands for 24 hours, is filtered with miillpore filter, the aperture of miillpore filter is 0.45 μm, precipitates 55 DEG C twice and dries to constant weight, cloud can be obtained
Radix Aucklandiae extract.
Embodiment 10: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, step 4 tool
Body is set as, and alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, and alcohol content is made to reach 75%, is ultrasonically treated 30min, ultrasound
The power of processing is 200W, frequency 30kHz, is centrifuged 10min, and the revolving speed of centrifugation is 2500rpm, takes supernatant liquor in 5 DEG C of ice
It stands in case for 24 hours, is filtered with miillpore filter, the aperture of miillpore filter is 0.45 μm, precipitates 55 DEG C twice and dries to constant weight, can obtain
To Aplotaxis auriculata extract.
Embodiment 11: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, in step 4,
The filtrate of concentration is pre-processed, and specifically comprises the following steps: for the filtrate of concentration to be placed in a container, is constantly being carried out
Potassium ferrocyanide solution and acetic acid zinc solution are added dropwise during stirring, until the turbidity of the filtrate of observation concentration is no longer
It improves, is filtered removal of impurities, obtains pretreated concentration filtrate.
Comparative example 1: a kind of extracting method of Aplotaxis auriculata extract, difference from example 1 is that, specifically include as
Lower step:
Step 1, stock, takes Aplotaxis auriculata raw material, dries at 50 DEG C to constant weight, is crushed, and crosses 45 meshes, obtains Yun Mu
Face powder end；
Step 2, polysaccharide water extract, Aplotaxis auriculata powder are placed in a container, extract 3 times, add 12 times of raw material weight for the first time
Water, second plus 10 times of raw material weight of water, third time plus 8 times of raw material weight of water, extraction time is respectively 120 minutes, 90
Minute, 60 minutes, combined extract；
Step 3, concentration, mixed extract is placed in centrifugal separator, is centrifuged 15min with 5000r/min, supernatant is taken to exist
It is concentrated under reduced pressure into the clear cream of relative density 1.22- at 60 DEG C, Aplotaxis auriculata extract is made.
Performance test sample: taking 3600g Aplotaxis auriculata raw material, be purchased from Anguo City Kant medicinal material Co., Ltd, and by its
12 parts are divided into, every part of 300g.
Test method: using the extracting method of 1 medium cloud Radix Aucklandiae extract of embodiment 1-11 and comparative example, respectively to 12 parts of clouds
Radix aucklandiae raw material extracts preparation, obtains 12 kinds of Aplotaxis auriculata extracts, then weighs 12 kinds of Aplotaxis auriculata extracts respectively
0.05g is dissolved in 50mL volumetric flask with deionized water, is measured 1mL into 10mL volumetric flask, is settled to quarter with deionized water
Degree, respectively takes 1mL into tool plug test tube, measures sample absorbance, calculates polyoses content according to normal equation.Polysaccharide extract rate=
[(Radix Aucklandiae extract amount × polyoses content)/sample quality] × 100%.
Test result: the test result of embodiment 1-11 and comparative example 1 is as shown in table 1.As shown in Table 1, by embodiment 1-3
Test result comparison with comparative example 1 can obtain, and after the dry crushing of Aplotaxis auriculata raw material, and obtain the more uniform cloud wood of granular size
Face powder end, is then first extracted repeatedly in hot bath with distilled water repeatedly, then carries out filtering and concentrating while hot to extracting solution, most laggard
Row alcohol precipitation is dry, being capable of polysaccharide extract rate with higher.Being compared by the test result of embodiment 4-6 and embodiment 1 can
, the pigment in Aplotaxis auriculata raw material can be removed with soaked in absolute ethyl alcohol to the Aplotaxis auriculata raw material after pulverizing and sieving.And then energy
The recovery rate of the polysaccharide enough greatly improved.Being compared by the test result of embodiment 7 and embodiment 1 can obtain, using methanol and
Formic acid mixing carries out alcohol precipitation drying, the recovery rate for the polysaccharide that can be greatly improved to concentrate.By embodiment 8-10 and embodiment
1 test result, which compares, to be obtained, and after ethyl alcohol is added in concentrate, carries out ultrasonic centrifugal treating, keep polysaccharide extract rate big
It is big to improve.Being compared by the test result of embodiment 11 and embodiment 1 can obtain, and potassium ferricyanide solution and acetic acid zinc solution can
The protein of the filtrate of concentration is set to generate precipitating.Be conducive to improve polysaccharide extract rate.
The test result of table 1 embodiment 1-11 and comparative example 1
| Extracting method | Polysaccharide extract rate/% |
| Embodiment 1 | 22.79 |
| Embodiment 2 | 22.83 |
| Embodiment 3 | 22.81 |
| Embodiment 4 | 24.84 |
| Embodiment 5 | 24.83 |
| Embodiment 6 | 24.86 |
| Embodiment 7 | 26.12 |
| Embodiment 8 | 25.32 |
| Embodiment 9 | 25.29 |
| Embodiment 10 | 25.31 |
| Embodiment 11 | 25.92 |
| Comparative example 1 | 11.12 |
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-described embodiment,
All technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
For those of ordinary skill, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of extracting method of Aplotaxis auriculata extract, which comprises the following steps:
Step 1, stock, takes Aplotaxis auriculata raw material, dries at 40-60 DEG C to constant weight, is crushed, and crosses 40-50 mesh, obtains
To Aplotaxis auriculata powder；
Step 2, polysaccharide water extract, Aplotaxis auriculata powder are placed in a container, and the distillation that 14-18 times of Aplotaxis auriculata powder is measured is added
Water, and water-bath is extracted 2-3 times at 70-80 DEG C, each 1.5-2.5h obtains extracting solution；
Step 3, concentration, extracting solution filter while hot, and obtained filtrate water-bath is concentrated into 0.5-1ml/g crude drug；
Step 4, alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, alcohol content is made to reach 70-80%, quiet in 4-6 DEG C of refrigerator
22-26h is set, is filtered with miillpore filter, 50-60 DEG C of precipitating dries to constant weight, and Aplotaxis auriculata extract can be obtained.
2. the extracting method of Aplotaxis auriculata extract according to claim 1, which is characterized in that the step 1 is specifically arranged
For stock takes Aplotaxis auriculata raw material, dries at 40-60 DEG C to constant weight, is crushed, and cross 40-50 mesh, then with anhydrous
Ethyl alcohol impregnates 5-10min, after being centrifuged air drying, obtains Aplotaxis auriculata powder.
3. the extracting method of Aplotaxis auriculata extract according to claim 1, which is characterized in that the step 4 is specifically arranged
For alcohol precipitation is dry, and methanol is added in the filtrate of concentration: formic acid (19:1) makes alcohol content reach 70-80%, in 4-6 DEG C of refrigerator
Middle standing 22-26h, is filtered with miillpore filter, and 50-60 DEG C of precipitating dries to constant weight, and Aplotaxis auriculata extract can be obtained.
4. the extracting method of Aplotaxis auriculata extract according to claim 1, which is characterized in that the step 4 is specifically arranged
For alcohol precipitation is dry, and ethyl alcohol is added in the filtrate of concentration, and alcohol content is made to reach 70-80%, is ultrasonically treated 20-30min, is centrifuged 5-
10min takes supernatant liquor to stand 22-26h in 4-6 DEG C of refrigerator, is filtered with miillpore filter, precipitates 50-60 DEG C twice and is dried to perseverance
Weight, can be obtained Aplotaxis auriculata extract.
5. the extracting method of Aplotaxis auriculata extract according to claim 4, which is characterized in that in the step 4, ultrasound
The power of processing is 200-250W, frequency 30-50kHz.
6. the extracting method of Aplotaxis auriculata extract according to claim 4, which is characterized in that in the step 4, centrifugation
Revolving speed be 2500-3500rpm.
7. the extracting method of Aplotaxis auriculata extract according to claim 1, which is characterized in that in the step 4, micropore
The aperture of filter membrane is 0.4-0.5 μm.
8. the extracting method of Aplotaxis auriculata extract according to claim 1, which is characterized in that in the step 4, to dense
The filtrate of contracting is pre-processed, and specifically comprises the following steps: for the filtrate of concentration to be placed in a container, is constantly being stirred
During potassium ferrocyanide solution and acetic acid zinc solution is added dropwise, until the turbidity of filtrate of observation concentration no longer mentions
Height is filtered removal of impurities, obtains pretreated concentration filtrate.
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