CN1104121A - Carious-tooth resistant compound and its preparation method - Google Patents
Carious-tooth resistant compound and its preparation method Download PDFInfo
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- CN1104121A CN1104121A CN 94107436 CN94107436A CN1104121A CN 1104121 A CN1104121 A CN 1104121A CN 94107436 CN94107436 CN 94107436 CN 94107436 A CN94107436 A CN 94107436A CN 1104121 A CN1104121 A CN 1104121A
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Abstract
The present invention provides a compound for prevention of dental caries, consisting of streptopeptide, theopolyphenol, oligosaccharide, taurine, xylitol, active calcium, conventional matrix and additive. The invented caries-resisting compound can effectively inhibit the growth and reproduction of oral pathogen mutant streptococcus, and can eliminate dental plaque and inhibit the formation of dental calculus. Besides, said medicinal compound has the advantages that it can quick clear-up saburra, promote the reproduction of intra-intestinal bifidobacteria, doesn't produce drug resistance and doesn't influence the equilibration of gastrointestinal flora.
Description
The present invention relates to be used for the pharmaceutical composition of caries prevention, particularly relating to and containing the streptococcus peptide is the anticaries compositions that mainly contains effective constituent, and preparation method thereof.
Dental caries mainly is the modal oral disease that is caused by the Streptococcus mutans in the oral cavity (Streptococcus nutens), particularly serotype C Streptococcus mutans.Its pathogenic approach is:
1, antibacterial adheres to and forms bacterial plaque on the enamel surface, and produces acid with the corrosion dentin; 2, bacteriogenic glucosyl transferase (GTF) makes monosaccharide remaining in the oral cavity change into glucose (a kind of mucopolysaccharide), the antibacterial of the latter in the oral cavity is deposited on periodontal and forms tartar, causes antibacterial anaerobic fermentation in tartar to produce acid and the corrosion tooth.Therefore, the approach of caries prevention should be to destroy the pathogenic activity of Streptococcus mutans and eliminate dental plaque and tartar.
Yet, because Streptococcus mutans belongs to sporeformer, fertility and Drug resistance are all very strong under oral environment, general chemical antibacterial medicines is difficult to reach sterilization effect, particularly can cause the physiology of oral cavity dysequilibrium as these medicines of life-time service, medicine enters gastrointestinal tract then can cause gastrointestinal tract interior dysbacteriosis and dysfunction.In addition, use sweeting agent replacement saccharide or use organic or inorganic salt inhibition oral cavity to cause caries prevention methods such as the dental caries factor, also be difficult to reach gratifying effect for reducing glucogenic condition.
Nisin (Wisin) is self mortifier that a kind of Streptococcus lactis (Lister) Lohnis 1909.554. (S.lactis) produces, and promptly admitted to be the avirulence food additive by the United Nations's health organization (WHO) food additive committee in 1969, be not reported before the present invention but use it for caries prevention.Known nearly all streptococcus all can produce similar bacterium peptide, though these peptide matters scope of restraining fungi are narrower, all has the selectivity bacteriostatic activity.The inventor finds in the research of seeking the dental caries medicine, wherein the bacterium peptide class that is produced by Streptococcus mutans is the most obvious to the inhibitory action of the thalline that produces it, and the bacterium peptide that various intersecting chain coccus produces all has bacteriostasis to the pathogenic bacteria of decayed tooth Streptococcus mutans.The inventor has furtherd investigate the material of several biogenetic derivations that act on dental caries morbidity link from the dental caries pathogeny, and through experiment repeatedly, it is the anticaries compositions of main active with the streptococcus peptide that preparation has finally been finished of the present invention.
Therefore; an object of the present invention is to provide a kind of compositions that is used for caries prevention, be characterised in that said composition is by forming as streptococcus peptide, tea polyphenols, oligosaccharide, taurine, xylitol, activated calcium and conventional medicinal substrate composition and the additive of main active ingredient.
According to anticaries compositions of the present invention; be characterised in that and contain streptococcus peptide 3500-4500 iu (IU), tea polyphenols 0.05-0.15g, beta-schardinger dextrin-0.4-0.9g in every 100g said composition: lactulose or other similar oligosaccharide 0.35-0.45g, taurine 0.0095-0.0105g, xylitol 14.0-15.0g, activated calcium 4.5-5.5g, remainder is suitable medicinal substrate composition and additive.
According to anticaries compositions of the present invention, wherein said streptococcus peptide is nisin or other similar streptococcus peptide.
According to anticaries compositions of the present invention, wherein said similar oligosaccharide comprises soybean oligo saccharide, rhizopus oligosaccharide and fructose, Raffinose etc.
Another object of the present invention provides the method for preparation according to anticaries compositions of the present invention; this method comprises that at first each component being ground into granularity is 120-200 purpose subparticle; successively oligosaccharide, streptococcus peptide, taurine and xylitol, activated calcium, tea polyphenols are added in the sterility container then; with each composition mixing, add substrate composition and other additives and the abundant mix homogeneously of the selection of q.s by blender at last.
The method according to this invention, the preparation that is characterised in that compositions are below 80 ℃, particularly finish under normal temperature condition.
The method according to this invention is characterised in that the preparation of compositions is finished under neutrality or slant acidity PH condition.
As the fundamental component of preventing decayed tooth compositions of the present invention, streptococcus peptide particularly nisin and Streptococcus mutans peptide is that the molecular weight about 3500 daltonian peptide classes that produced by Streptococcus lactis (Lister) Lohnis 1909.554. and Streptococcus mutans are respectively produced bacterium self inhibiting substances.We find that Streptococcus mutans is very responsive to this class streptococcus peptide, and micro-bacterium peptide can produce the thalline breeding and suppress active significantly.Its bacteriostasis mainly is to influence cell membrane and stop the synthetic of bacteria cell wall as cationic surfactant.This class bacterium peptide is invalid to bacterial spore, but sprouts when spore, and the pre-excrescence stage of expanding then can be brought into play strong inhibitory action.This antibacterial behavior can long-term co-existence or the contact of long-term low concentration not be developed immunity to drugs with it because of antibacterial.The oral cavity Streptococcus mutans that suppressed by nisin or other chain-like coccus peptides, tackability significantly reduces when losing biologic activity, show as dental plaque and through mechanism friction (as brushing teeth) time, will strip off at an easy rate, and new dental plaque is difficult to form.Making us interested especially is, these bacterium peptides will be hydrolyzed into aminoacid rapidly in gastrointestinal tract, and the ecological balance to gastrointestinal bacterial flora brings adverse effect other antibiotic substance and can not resemble.
The tea polyphenols that is used for the present composition is the active ingredient of Folium Camelliae sinensis, and it has good non-oxidizability, is again the edible composition of natural origin.We find that under study for action tea polyphenols and streptococcus peptide have collaborative dental caries effect, and both effects of use in conjunction obviously are better than using separately wherein any.Simultaneously, tea polyphenols can suppress sulphydryl activity, so fairly obvious to eliminating the halitosis effect that is caused by methanthiol in the oral cavity.
The introducing of beta-schardinger dextrin-mainly is to be used for protection and stable streptococcus peptide class in the present composition, makes it have the uniform slow release function, thereby guarantees can deliver medicine to the flank of tooth whole chewing in the process.Experiment showed, and use the gum that contains this component can make active substance in 5 minutes, can in the oral cavity, maintain valid density.
Lactulose claims isomerized lactose again, belongs to a kind of oligosaccharide, and it and soybean oligo saccharide etc. similarly oligosaccharide have the putrefaction bacteria that suppresses enteral, reconciles the bacillus bifidus function of propagation rapidly.This quasi-microorganism that is present in human small intestinal lower end and the large intestine can synthesize vitamin B group and some aminoacid in intestinal.People confirm that also the minimizing of bacillus bifidus is proportionate in human aging and the intestinal.Add general Chinese meal recipe crowd, because the unreasonable and monophagia of diet structure distributes crowd's intestinal flora and lacks of proper care and the vitamin B group shortage.The accumulation of this amount will cause the decline of body constitution.Show as anorexia and monophagia for the child, then show as easy trouble gastrointestinal tract inflammation and health premunition for adult and old people and weaken etc.In addition, from the epidemiology angle, general vitamin B group was taken in low area, dental caries prevalence all higher (as the difference in rural area and city).Introducing similar oligosaccharide such as lactulose in the compositions of the present invention mainly is to reconcile function of human body, promotes the absorption and the utilization of nutrient substance (comprising calcium constituent), provides necessary assistance to the anti-dental caries of human body.
The taurine that adds in the present composition mainly plays nutrition periodontal nerve, improves effects such as oral cavity blood microcirculation, antiinflammatory.
Activated calcium be taking from of can recognizing of present people natural in human body, absorb preferable calcium-supplementing preparation.The activated calcium main purpose that adds in the present composition as auxiliary composition is to replenish calcium, promotes tooth development.
Xylitol is the natural sweetener that a kind of sugariness is equivalent to multitudinous sugar, has the people once to be used for caries prevention.A key property of xylitol is to have very strong hydration.The hydration of xylitol can make the good activated calcium of water solublity form the calcic hydration shell at dental surface, so as to taking care of one's teeth, promotes calcareous mineralising, and then improves the resistance against diseases of tooth.
All compositions of this compositions all can be bought on market.The Streptococcus mutans that this research relates to is known strain, can be available from Shanghai Second Emdical University's stomatology institute as specific bacterial strain.Promptly as specific bacterial strain.Nisin (Wisin) can be buied from the Applin and barett ltd. of Britain.
Can prepare anticaries compositions of the present invention according to a conventional method.This method is included in next sterile chamber of room temperature earlier the 0.4-0.8g beta-schardinger dextrin-with a small amount of sterilized water furnishing pasty state; again 0.0095-0.0105g taurine, 3500IU-4500IU streptococcus peptide were put into this container fully mixing 5-15 minute; with 0.3-0.5g lactulose (or other similar oligosaccharide); 0.45-0.55g activated calcium was put into wherein also abundant mixing 5-10 minute; add 14-15g xylitol and 0.05-0.15g tea polyphenols and abundant mixing again, add other excipient and additive at last to whole weight 100g.
Best product form of the present invention is a gum.Its preparation method is in the temperature control kneader 20kg semi-finished product gum base to be softened, and drops into 35kg120 order above white sugar and 20kg high fructose syrup.Between 80 ℃-100 ℃, be stirred well to evenly.When temperature of charge is lower than 80 ℃ of-30 ℃ of room temperatures; adding is by 0.6kg beta-schardinger dextrin-, 0.01kg taurine, 4,000,000IU streptococcus peptide; 0.4kg lactulose; the 5kg activated calcium, 15kg xylitol, the compositions that the 0.1g tea polyphenols is formed and pressed the preceding method mixing; add an amount of spice plasticizer etc. again; blending is evenly cooled off extrusion modling and stripping and slicing or granulating and forming.Finished product must be preserved in the lucifuge sealing.
Anticaries compositions of the present invention can add appropriate excipients and other additives as required to be made the gum of chewing usefulness, the solution that contains the usefulness of coughing and dental surface and is coated with binding agent of application etc., can certainly be added in and make medicated toothpaste in the toothpaste matrix.Wherein preferably add colloidal stroma and sweeting agent is made gum.
The additive that is applicable to anticaries compositions of the present invention can also comprise the conventional additives such as solubilizing agent, coloring agent, excipient, sweeting agent and spice that use except that figuration substrate.Can be as required, in anticaries compositions of the present invention, add different substrates composition (excipient) and other suitable additives, as add the glue nurse and make gum, add water-based and make gargarisma, add cellulose or other binding agents and make tooth and/or gum surfaces Liniment and anticarious tooth paste etc.
Anticaries compositions of the present invention is mainly used in the human dental caries of prevention, and it has, and fungistatic effect is remarkable, the advantage of no Drug resistance.In general, as long as established cavity does not reach the degree of exposing dental nerve as yet, all can stop focus activity and development effectively.Because active ingredient bacteriostatic effect in the present composition is so can effectively and promptly remove and suppress dental plaque.In addition,, cause the GTF inactivation, make the monosaccharide in the oral cavity can not change into mucopolysaccharide, thereby lost the basis that forms tartar, reach and suppress the purpose that tartar generates because present composition streptococcus peptide has suppressed bacterial growth.Moreover, since compositions of the present invention can be rapidly, suppress effectively or eliminate most of methanthiol material in the oral cavity that produces halitosis, so can eliminate halitosis effectively.At last, owing to do in the present composition can be degraded to aminoacid very soon after the streptococcus peptide of primary activity composition enters the interior gastronintestinal system of human body, and other active ingredients in the compositions also are (as detailed above) of natural edible plant and animal material, therefore said composition life-time service safety is paid effect without any poison.
The preparation method and the advantage thereof of anticaries compositions of the present invention are further described for example below in conjunction with embodiment.
Embodiment 1: the best preparation of anticaries compositions
Present embodiment has been described the preparation method of the gum that contains anticaries compositions of the present invention.At first adding 35g120 order is with upper level Saccharum Sinensis Roxb. powder in the softening good gum base of 20g (can available from the colored gum base in Huifeng, Jiangsu factory), and the 20g high fructose syrup stirs under 80 ℃ of-105 ℃ of temperature.Reduce to 25 ℃-80 ℃ following times at temperature of charge; add and contain the 0.6g beta-schardinger dextrin-, the 0.01g taurine, 4000IU (about 100 μ g) streptococcus peptide is (available from Britain; Applin and barett LTO); 0.4g lactulose, 5g activated calcium, 15g xylitol; 0.1g pressing in the blended compositions of preceding method of tea polyphenols; add an amount of spice and plasticizer again, blending is evenly cooled off back extrusion modling and stripping and slicing and is shaped.
Embodiment 2: bacteriostatic experiment
Present embodiment has been described the bacteriostatic test and the result thereof that use Streptococcus mutans to carry out.Now be described below respectively:
I), bacteriostatic experiment:
Be inoculated in the new broth bouillon after serotype C146 bacterial strain (can buy from Shanghai Second Emdical University's stomatology institute) bred in broth bouillon, cultivated 24 hours for 36 ℃,, abandon supernatant with centrifugal 10 minutes of 300r/min centrifuge.With sterile saline precipitate is diluted to the bacterium liquid of Maxwell reduced turbidity 0.5, redilution become 100 times standby.In the 5ml broth bouillon, (contain about 30 of mutans streptococcus approximately), add the present composition and make it contain the streptococcus peptide concentration to be respectively 20ppm, 10ppm, 5ppm, 0ppm; This solution (PH6.9) was being cultivated 18 hours down through 36 ℃.The test tube culture fluid is shaken up, be seeded in respectively and evenly be paved into 1cm diameter circle on the blood agar culture-medium.Cultivated 24 hours through 36 ℃, judge bacteriostatic level by following standard: " ++ ++ " is whole inactivations, asepticly is born; " +++" be obviously to suppress, visible minority bacterium colony; " ++ " is that part suppresses, and bacterium colony has been linked to be sheet, but still visible part obviously denumerable be dispersed in bacterium colony; "-" suppresses, and bacterium colony merges in flakes, and be same with matched group.The result shows that the compositions that contains 10ppm concentration streptococcus peptide can all suppress the pathogenic bacterium growth, promptly be judged as " ++ ++ ".
II) bacteriostatic time effect:
Add in the 2ml broth bouillon with the bacterium liquid of preceding method, make bacterium liquid be diluted to suitable multiple Maxwell turbidity 0.5.Timing adds the composition solution that final concentration contains 10ppm streptococcus peptide, after adding bacteriostatic solution 1,2,3,4,5,6,10,15 etc. minutes, draw the bacterium liquid 0.1ml of mixing respectively, add in the 0.9ml broth bouillon (active ingredient is released to containing below the streptococcus peptide 0.1ppm), get about 1ml then, be added drop-wise on the blood plate,, and observe the bacterial growth situation in 36 ℃ of cultivations 24 hours.When the result is 5 minutes, there has not been visible pathogenic bacterium bacterium colony, promptly be judged as " ++ ++ ".
Embodiment 3: quick antihalitosis laboratory observation
Present embodiment is to estimate compositions of the present invention to the clearance rate of methanthiol and describe crowd's observation of curative effect result by the simulation oral conditions.Its method is: add the saliva substrate 5ml that contains the 0.5mg methionine in the 25ml conical flask, put 37 ℃ of waters bath with thermostatic control and cultivated 24 hours, press known method, use gas chromatograph for determination content.Then, add the present composition of normal working concentration in quantitative methanthiol culture medium, 37 ℃ kept 5 minutes, and the reuse gas chromatography is surveyed its content.With peak area the slip of methanthiol is converted into the smelly rate that disappears.The result shows that the smelly rate that on average disappears is more than 85%.
In addition, by chewing the gum of this compositions, subjective reflection deodorizing effect preferably is 100%.Simultaneously, the abnormal flavour that Bulbus Allii, cigarette, wine etc. are produced in the oral cavity also has remarkable deodorization.
Embodiment 4: remove the dental plaque ability and observe
Present embodiment has been described crowd's trial effect of the oligodendrocyte glycoprotein form of the present composition and has been observed.The result is shown in back embodiment 6 invading the exterior one.
Statistical result shown in the table one shows, uses the optimal formulation gum of the present composition, has the very strong dental plaque ability of removing.Illustrate and use the present composition that the pathogenic bacterium adhesion strength is significantly failed, dental plaque is easy to peel off.
Embodiment 5: the Drug resistance experiment
Whether present embodiment mainly is to observe pathogenic bacterium developing immunity to drugs under the low concentration stimulation repeatedly, its experimental technique is: known mutans streptococcus is inoculated in the broth bouillon of the present composition that contains 0.1ppm streptococcus peptide, cultivated 24 hours for 36 ℃, antibacterial transferred species after cultivating is contained in the broth bouillon of same concentration subculture to another.So repeated inoculation is cultivated 10 times.To take out through the Streptococcus mutans of cultivating in same concentration present composition mixed liquor for 10 times, the menses plate is streak culture again, and microscopy does not have living contaminants.Make a matched group with this bacterium and participate in bacteriostatic experiment (method is with embodiment 1), result and the same bacterium source strain zero difference that does not experience the Drug resistance experiment.
Embodiment 6: crowd's observation of curative effect
Select 18-35 single employee between year at random.These people have meal in same dining room, and health status, living habit and food chain are roughly close.Study subject is divided into experimental group and matched group at random.Every group of each 90 people of initial stage, 30 days experimental periods, every group is no less than 70 people when experiment finishes.By the classroom teaching, two groups of toothpaste of the same race that all use same model toothbrush and do not contain any medicine are brushed teeth in unification by standard method.Per 10 days of toothbrush is changed once, through the training of brushing teeth of one-week standard, and to fill in a form after once all experimenters' of record the natural situation in oral cavity of every clear tooth dirt of experimenter.Test rating is:
1. dental plaque index, 2. gingival index (plaque index and gingival index are all determined by the standard that Silness and loe propose).Experimental session requires the experimenter to brush teeth just before going to bed once every day, gargles once after meal at every turn, and chews one of this product chewing gum (5g/ piece).Matched group (blank group) is given comfort type chewing gum (same base material, color do not contain present composition active ingredient).Timing was chewed 5 minutes/time without exception.Every day secondary, all half an hour, chew after the meal.Carry out dental plaque and gingiva after 30 days and recheck, and interpretation of records, carry out statistical analysis and handle (the results are shown in Table one, table two).Wherein bacterial plaque dyes with basic fuchsin; Gingiva is checked with the standard periodontal probe.Check that the tooth position is four facings of 6 teeth such as 14,16,21,24,30,32.
Table one plaque index
Plaque index
After being tried before group example number is tried
Experimental group 84 1.164 ± 0.124 0.345 ± 0.21
Matched group 72 1.172 ± 0.211 0.876 ± 0.115
Compare t check, P＜0.001 before and after being tried in the experimental group.
After being tried, experimental group and matched group compare, t check, P＜0.001.
The result shows shown in the table one, and compositions of the present invention has stronger elimination bacterial plaque and suppresses tooth bacterium formation effect.
Table two gingival index
Gingival index
After being tried before group example number is tried
Experimental group 84 1.011 ± 0.022 0.5870 ± 0.163
Matched group 72 1.048 ± 0.166 0.9882 ± 0.141
Compare t check, P＜0.001 before and after being tried in the experimental group group.
After being tried, experimental group and matched group compare, t check, P＜0.001.
Result shown in the table two shows that the present composition does not only influence the oral cavity and gives birth to too environment, and has prevention and suppress oral inflammation, the effect of protection oral cavity.
Claims (6)
1, a kind of anticaries compositions is characterised in that said composition is by forming as streptococcus peptide, tea polyphenols, beta-schardinger dextrin-, lactulose or similar oligosaccharide, taurine, xylitol and the activated calcium of primary activity composition and conventional substrate composition and additive.
2, according to the anticaries compositions of claim 1; be characterised in that in every 100g said composition and contain streptococcus peptide 3; 500-4; 500 ius, tea polyphenols 0.05-0.15g, oligosaccharide 1.0-1.5g, taurine 0.0095-0.0105g, xylitol 14.0-15.0g, activated calcium 4.5-5.5g, all the other are suitable substrate composition and an amount of typical additives.
3, according to the anticaries compositions of claim 1 or 2, wherein said streptococcus peptide is selected from nisin and Streptococcus mutans peptide.
4, according to the anticaries compositions of claim 1, wherein said similar oligosaccharide comprises soybean oligo saccharide, rhizopus oligosaccharide and fructose.
5, a kind of method for preparing anticaries compositions; feature is included in next sterile chamber of room temperature earlier the 0.4-0.8g beta-schardinger dextrin-with a small amount of sterilized water furnishing pasty state; again with the 0.0095-0.0105g taurine; 3500IU-4500IU streptococcus peptide was put into this container fully mixing 5-15 minute; 0.3-0.5g lactulose (or other similar oligosaccharide) 0.45-0.55g activated calcium was put into wherein fully mixing 5-10 minute; add 14.0-15.0g xylitol and 0.05-0.15g tea polyphenols and abundant mixing again, add other excipient and additive at last to whole weight 100g.
6,, be characterised in that said preparation finishes under 25 ℃ of-80 ℃ of conditions according to claim 1,5 method.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94107436A CN1066960C (en) | 1994-07-19 | 1994-07-19 | Carious-tooth resistant compound and its preparation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN94107436A CN1066960C (en) | 1994-07-19 | 1994-07-19 | Carious-tooth resistant compound and its preparation method |
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| CN1104121A true CN1104121A (en) | 1995-06-28 |
| CN1066960C CN1066960C (en) | 2001-06-13 |
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| CN94107436A Expired - Fee Related CN1066960C (en) | 1994-07-19 | 1994-07-19 | Carious-tooth resistant compound and its preparation method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003086359A1 (en) * | 2002-04-17 | 2003-10-23 | Jiang Yu | Tea polyphenol buccal tablet for preventing and treating tooth decay and its preparing method |
| CN100403925C (en) * | 2003-06-18 | 2008-07-23 | 上海同利实业有限公司 | Sugar-free candy compositions for replenishing the calcium and its preparation process |
| CN103385805A (en) * | 2013-07-18 | 2013-11-13 | 广州薇美姿个人护理用品有限公司 | Composite children toothpaste suitable for use in the morning and in the evening and children toothpaste suitable for use in the evening |
| CN105920202A (en) * | 2016-03-26 | 2016-09-07 | 广东润和生物科技有限公司 | Coenzyme Q10 gum for protecting teeth, strengthening brain and removing odor |
| CN107106446A (en) * | 2014-12-23 | 2017-08-29 | 高露洁-棕榄公司 | Oral care composition |
| CN113950330A (en) * | 2019-03-11 | 2022-01-18 | 泡泡奇力有限公司 | Compositions and methods for inhibiting the growth of bacteria that cause dental caries, gingivitis and bad breath |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9126686D0 (en) * | 1991-12-17 | 1992-02-12 | Smithkline Beecham Plc | Novel compositions |
| ATE145817T1 (en) * | 1992-09-10 | 1996-12-15 | Sara Lee De Nv | ORAL CARE PRODUCT WITH ANTIBACTERIAL ACTIVITY |
| GB9224598D0 (en) * | 1992-11-24 | 1993-01-13 | Smithkline Beecham Plc | Novel compositions |
-
1994
- 1994-07-19 CN CN94107436A patent/CN1066960C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003086359A1 (en) * | 2002-04-17 | 2003-10-23 | Jiang Yu | Tea polyphenol buccal tablet for preventing and treating tooth decay and its preparing method |
| CN100403925C (en) * | 2003-06-18 | 2008-07-23 | 上海同利实业有限公司 | Sugar-free candy compositions for replenishing the calcium and its preparation process |
| CN103385805A (en) * | 2013-07-18 | 2013-11-13 | 广州薇美姿个人护理用品有限公司 | Composite children toothpaste suitable for use in the morning and in the evening and children toothpaste suitable for use in the evening |
| CN103385805B (en) * | 2013-07-18 | 2016-06-29 | 广州薇美姿实业有限公司 | A kind of benefit is benefit combination children's toothpaste in evening and one benefit children's toothpaste in evening early |
| CN107106446A (en) * | 2014-12-23 | 2017-08-29 | 高露洁-棕榄公司 | Oral care composition |
| CN107638303A (en) * | 2014-12-23 | 2018-01-30 | 高露洁-棕榄公司 | Oral care composition |
| CN105920202A (en) * | 2016-03-26 | 2016-09-07 | 广东润和生物科技有限公司 | Coenzyme Q10 gum for protecting teeth, strengthening brain and removing odor |
| CN113950330A (en) * | 2019-03-11 | 2022-01-18 | 泡泡奇力有限公司 | Compositions and methods for inhibiting the growth of bacteria that cause dental caries, gingivitis and bad breath |
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|---|---|
| CN1066960C (en) | 2001-06-13 |
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