CN105726350B - A kind of toothpaste containing Pinus massoniana - Google Patents
A kind of toothpaste containing Pinus massoniana Download PDFInfo
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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Abstract
The present invention relates to a kind of toothpaste containing Pinus massoniana, and the wherein weight ratio of active ingredient lysozyme and Pinus massoniana extract is (1:10)~(10:1).Compared with common Chinese herbal toothpaste, toothpaste preparation method of the invention is simple, and compatibility is reasonable, and two kinds of active components can produce synergistic function while respective effect is played, and significantly prevents oral plaque and gingivitis.Lysozyme and Pinus massoniana source are natural, safe and non-toxic, without side-effects；Due to having no drug resistance in toothpaste without western medicine composition, toothpaste.
Description
Technical field
The present invention relates to oral hygiene field, and in particular to a kind of toothpaste.
Background technology
It will medically surround and cover and be known as gum in periodontal soft tissue, the acute and chronic for betiding gingiva tissue is scorching
Disease is known as gingivitis.Bleeding gums are shown as, red and swollen, distending pain, continues development and invade sclerous tissues, produces periodontitis, including gum
Performance of the inflammation and general disease of tissue in gum.Plaque is the adhered to one another of matrix parcel or is adhered between facing, tooth
Or the bacillary colony of the soft and non-mineralising on dummy surface, for a kind of Bacterial biofilms fallen cannot be washed away or gargled by water.
When plaque amount is less, it is difficult what is observed to be visually, can usually be shown well with plaque indicator agents.
Toothpaste is a kind of preparation that auxiliary is brushed teeth, and can strengthen the frictional force brushed teeth, and help removes food scraps, soft dirt and tooth
Bacterial plaque, helps to eliminate or mitigate oral peculiar smell.By mechanical methods, plaque or prevention gingivitis are thoroughly removed to big
It is difficult to accomplish for majority.Therefore, researcher proposes to add certain antiseptic in toothpaste.At present, it is used to prevent on the market
The antiseptic controlled in the feature toothpaste of gingivitis and plaque is mainly Western medicine, and long-time service easily produces drug resistance.Biology
Enzyme toothpaste is a kind of toothpaste containing the component such as bioenzyme activity component and enzyme stabilizers, activator, it can be further
Bactericidal, promotes oral cavity further to clean, so as to take care of one's teeth, strengthens the defense system in oral cavity itself.
Lysozyme (lysozyme) is also known as muramidase (muramidase) or N-acetylmuramide lycanohydrlase (N-
Acetylmuramide glycanohydrlase), it is a kind of alkaline enzyme that can be hydrolyzed and polysaccharide is sticked in pathogenic bacteria.Mainly by broken
The β between -acetylmuramic acid and n-acetylglucosamine-Isosorbide-5-Nitrae glycosidic bond in bad cell wall, makes cell membrane insoluble glutinous more
Sugar decomposition causes cell wall rupture content to escape and make bacterolysis into soluble glycopeptide.Lysozyme can also with it is negatively charged
Virus protein directly in conjunction with, with DNA, RNA, apoprotein formed double salt, make virally inactivated.Therefore, the enzyme have antibacterial,
Anti-inflammatory, antiviral etc. act on.Lysozyme (also referred to as lyase) is the enzyme that a molecular weight is 14.4kDa, it can pass through catalysis
In peptide glycan between -acetylmuramic acid and N-acetylglucosamine residue and in chitodextrin between N-acetyl-glucosamine residue 1,
The hydrolysis of 4- β chains, and destroy the cell membrane of bacterium.In human body, lysozyme is in cell juice, as saliva, tears and its
It is widely present in his some body fluid；Exist in the cytoplasmic granule body in mitochondria.In addition, also have in egg white substantial amounts of molten
Bacterium enzyme.Lysozyme is widely used because of the alkali hydrolysising protease of the cell membrane for acting on purpose microorganism that can be single-minded
In industries such as food, feed, medicine.
By the simple alkaline globulin of 18 kinds of 129 Amino acid profiles, molecular weight is lysozyme (lysozyme)
14.4KDa.Chemical property is highly stable.When pH value is acute variation in the range of 1.2~11.3, its structure is almost unchanged.
In sour environment, lysozyme is very strong to the stability of heat；When pH value is 4~7,100 DEG C of processing 1min remain to keep protoenzyme to live
Property；100 DEG C of heating 45min are resistant to when pH value is 3；In dry conditions, lysozyme can be stored in room temperature for a long time, its
Sterling is white or yellowish.The crystalline solid or amorphous powder of yellow, it is odorless, it is sweet.It is soluble easily in water, by alkali destructible, no
It is dissolved in acetone and ether.As medical supplies, lysozyme have the advantages that good biocompatibility, to organize it is non-stimulated, non-toxic.
Lysozyme is widely present in the poultry, tear of the egg white of birds and mammal, saliva, blood plasma, urine, milk, white
Cell and other body fluid and tissues (such as liver, kidney) are intracellular；From the plant such as barley, fig, pawpaw and cabbage, radish
Lysozyme can be isolated, wherein using content in egg white as highest (containing about 0.3%).And the bacteriolyze enzyme activity in human milk, tears, saliva
Property be far above the activity of the lysozyme in (about 3 times) or other sources in egg white.Lysozyme in egg white is animal lysozyme
Typical Representative, be research object important in lysozyme group, and it is currently understood that one of most clear lysozyme.Moved in lactation
In the milk of thing, the lysozyme content in human milk is 3000 times of cow's milk.It is by sources different, it is molten lysozyme can be divided into egg white
The lysozyme that the lysozyme and bacteriophage that bacterium enzyme, animal lysozyme, plant lysozyme, microorganism produce produce；By effect
Cell membrane is different, and lysozyme can be divided into bacteria cell wall lysozyme and fungal cell wall lysozyme.
Lysozyme is capable of the cell membrane of single-minded cracking purpose bacterium.Its main attack peptide glycan, hydrolysis connection N- acetyl born of the same parents
β -1,4 glycosidic bonds of the 4th carbon atom of Teichaic acid and N-acetyl-glucosamine.Whole process is that lysozyme is tied by two first
" ditch " between structure domain is attached on peptide glycan molecule；Its subsequent substrate forms the conformation of transition state in enzyme.According to
Phillips mechanism, lysozyme are combined with glucohexaose.Then the 4th sugar distortion on glucohexaose is half chair by lysozyme
Shape conformation.In this twisted state (energy is higher), glycosidic bond is easy for being broken.Positioned at antalzyme protein sequence 35
The glutamic acid (Glu35) of position and the side chain of the aspartic acid of 52 (Asp52) are very crucial to the activity of lysozyme.Glu35 makees
For the proton donor of glycosidic bond, the C-O keys of substrate are sheared；And Asp52 participates in generation glycosyl enzyme intermediate as nucleopilic reagent.With
Afterwards, glycosyl enzyme intermediate reacts with hydrone, hydrolysis generation product, and enzyme remains unchanged.
The extracting method of lysozyme includes following several：1st, direct crystallization method：This method is easy to operate, and cost is low, but cannot
To separate micro lysozyme.2nd, ion exchange chromatography：This method has simplicity, efficient, cost is low, and can automate continuous
The advantages of operation, encloses.3rd, affinity chromatography：Multiple that affinity chromatography isolates and purifies is higher, quality is preferable, and can be widely applied to
The lysozyme in various sources, for concentrating and refining micro lysozyme.But since the making of affinity adsorbent is more complicated, limit
Its extensive utilization industrially is made.4th, ultrafiltration is combined with affinity chromatography：Ultrafiltration is combined extraction lysozyme with affinity chromatography,
It is the new method developed in recent years.Most of foreign protein is removed with ultrafiltration first, retains lysozyme, lysozyme is also relatively thick at this time,
Again purified product is obtained with affinity chromatography.5th, membrane chromatography technology：, there is affinity film chromatography technology in late 1980s.
The technology has UF membrane and affine the characteristics of separating concurrently.Compared with traditional UF membrane, affinity chromatography, membrane chromatography technology not only has
There have that purification is high, pressure drop is small, disengaging time is short, large biological molecule be denatured probability in separation process to be small, and allows comparatively fast
Charging rate the features such as, and be more easy to accomplish scale production than column affinity chromatography.6th, ultrafiltration：With traditional bio-chemistry separation skill
Art is compared, and hyperfiltration technique is a major advantage that the high yield output of product.But although hyperfiltration technique was waited in reverse osmosis and concentration
It is widely used in journey, but a kind of protein stripping technique very big as the potential in biological industry field, ultrafiltration
It is not fully utilized yet.7th, reverse micelle extracts：Reverse micelle extraction is a kind of new extracting process that developed recently gets up, it is profit
With reverse micelle that a small amount of surfactant formed is added in organic solvent come the method extracted, organic solvent cannot be used for some
The bioactive substances such as the enzyme and reactive protein of extraction have opened up a kind of new isolation technics, expand organic solvent extraction
The scope of application.8th, bioseparation technology：Bioseparation technology based on affinity is (immobilized metal affinity chromatography, affine heavy
Form sediment, affinity filtration method), and one of lysozyme extracting method.
Lysozyme important pharmacological action with a variety of unique.
First, antibacterial anti-inflammatory.The cell membrane of bacterium is made of murein, and murein is by N-acetylglucosamine and N-
Acetylmuramic acid replaces the polymer of composition, can be known as peptide glycan with connecting peptides on cell wall residue.Polysaccharide is deposited with linear form
It can be connected with each other between polysaccharide chain located adjacent one another by peptide chain part, so as to form three-dimensional structure.Bacteriolyze enzyme specificity
Ground acts on the glycosidic bond between the -acetylmuramic acid of peptide glycan molecule and N- acetyl glucosamine ammonia, as a result makes bacteria cell wall
Become loose, lose the protective effect to cell, last cell dissolution is dead；Lysozyme can directly kill gram positive bacteria,
Under the participation of S-IgA complement, moreover it is possible to kill gram-negative bacteria：Such as escherichia coli.Lysozyme can also with it is each
The acidic materials that kind induces inflammation combine, and reduce bacterial endotoxin release, so as to mitigate endotoxemia, slow down inflammation and send out
Raw process, and certain synergistic effect is known as to antibiosis, improve glutinous more glycometabolisms of periplast, play antibacterial anti-inflammatory, reparation group
The effect knitted.Tests prove that being coupled human lysozyme and proteolytic enzyme with a kind of cellulose copolymer, it is remarkably improved molten
Bacterium enzyme antibacterial ability, accelerating wound healing.
Second, it is antiviral.Lysozyme carries a large amount of positive charges under neutral fluid environment, can be with the virus with negative electrical charge
Albumen directly acts on, and is combined to form double salt with DNA, RNA, desorption base albumen, makes virally inactivated.By herpesvirus infection
Have in Hela cell culture fluids plus after lysozyme and suppress cell degeneration effect.Lysozyme also can inhibit adenovirus growth, can be used for
The viral illness treatment such as herpes zoster, parotitis, chicken varicella, hepatitis and influenza.
3rd, strengthen immunity.Lysozyme participates in panimmunity reaction in body, in body normal defense and non-specificity
Play an important roll in immune.It can improve and strengthen macrophage phagocytosis digestive function, reduce white caused by cytostatics
Leukopenia, with reference to bacteria lipopolysaccharide, mitigates endotoxin effect, to achieve the purpose that to strengthen Abwehrkraft des Koepers.Lysozyme is in itself
With t cell epitope, the reaction of 2 types (Th2) t helper cell can induce.Oral lysozyme can induce mouse produce systemic Th1 and
Th2 immune responses, strengthen immune function, and therefore, oral lysozyme has the function of to control the infection of the upper respiratory tract and treats dysentery.
In addition, lysozyme also has the function of that activation is hematoblastic, tissue local blood circulation disorder can be improved, decompose fester, enhancing office
Portion's defense function, so as to embody its hemostasis, detumescence and the repair for accelerating body injury tissue.It is alternatively arranged as a kind of host
Resistance factor, shields tissue local.
Lysozyme is killed in food fresh keeping anti-corrosion, oral cavity disinfecting, Water Disinfection, air purification and sterilizing and household electrical appliance
It is widely used in the fields such as bacterium disinfection.Research finds that lysozyme can be used for preservative of chilled meat, which uses completely
Safe and nontoxic, crude scientifically with being combined into, can extend 1-4 times of the shelf-life of chilled meat.The antistaling agent can be resistance to
By the temperature below 95 DEG C, and retention properties is stablized.Lysozyme can also be used for the anti-corrosion of dairy products, add during cheese processing
A certain amount of lysozyme is added to prevent blistering, being spoiled for middle and later periods cheese；It can also keep cheese base in cheese ageing process
The quality of liquid, it is often more important that it can play long-term bactericidal effect, effectively control the fermentation of butyric acid.In addition, bacteriolyze
Enzyme can also be used for sterilizing of the anti-bacterial refreshing of food flexible packing material, the anti-corrosion of wine and beverage, aquatic products etc..
Bacterium in air is adsorbed on dirt particle, and common pathogenic bacteria have Mycobacterium tuberculosis, Corynebacterium diphtheriae bar
Bacterium, bordetella pertussis, staphylococcus aureus, micrococcus scarlatinae, Neisseria meningitidis, Legionella etc., can cause breathing
Road transmission disease or wound infection.In view of safety factor, the filter such as generally use glass fibre HEPA filters air
Purify degerming.However, microorganism easily breeds on HEPA, disperses, secondary pollution is formed.Domestic someone has studied successfully one kind
Lysozyme PPHEPA filters, lysozyme is immobilized on the whole filter paper fibre of HEPA by it, can be caught and be killed in fibrous inside
Microorganism killing, effectively prevent secondary pollution.
Health type household electrical appliance are the trend and trend of current household electric appliances.It is applied to the health of white goods industry at present
Sterilization technology using tradition and physical sterilization and chemical disinfection, there are security risk, secondary pollution problems more.Will be molten
The sterilization that bacterium enzyme is applied to white goods industry can solve the problems, such as this respect well.Lysozyme sterilization technology is in sky at present
Gas cleaner, central air-conditioning, have tentative application on purifier.
Bacterium is the main reason for mouth disease occurs, and dental caries, pulpitis, periapical inflammation and part mucous membrane disease are all and oral cavity
The infection of bacterium is inseparable.Lysozyme is antimicrobial component important in people's saliva, participates in nonimmune defence of the host to bacterium,
The homeostasis of oral environment is adjusted, the occurrence and development with resisting mouth disease have certain relation.Lysozyme can suppress oral cavity
The growth of carious tooth, suppresses thalline glycolysis and production acid, so most scholars think that lysozyme has certain effect to pre- anti-caries.This
Outside, lysozyme can also treat canker sore, while can also can also be used as cleaning agent for mouth cavity, take care of one's teeth and oral health.
The healing of canker sore with repair tissue, can be conducive in oral cavity using lysozyme.Staphylococcus lysozyme and synergist, stabilizer
It may also be fabricated which mouth cleaning solution, several pathogenic bacteria for causing mouth disease, such as staphylococcus, hemolytic streptococcus, white read
Pearl bacterium, anaerobic bacteria etc. have inhibitory or killing effect.By detection, sterilizing rate is up to 99.9% in 5min.Acute toxicity test, cumulative toxicity examination
Test, the safety evaluatio such as micronucleus test, mucosa irritation test meets the requirements.Extracorporeal disinfecting, which is tested, to be shown, the compound system of lysozyme
Agent can effectively suppress and kill pars oralis pharyngis pathogenic bacteria, can effectively treat the mouth diseases such as acute/chronic pharyngitis, tonsillitis.Due to
Treatment and prevention of the lysozyme to mouth disease have the effect of fine, existing lysozyme lozenge listing domestic at present, lysozyme
There is very wide prospect in terms of mouth disease.
Masson pine Pinus massoniana Lamb., also known as pine tree, belong to gymnosperm Pinaceae Pinaceae Pinus
Pinus, is a kind of aiphyllium, high up to 30 meters.The leaf of masson pine is in aciculiform, practises and claims pine needle, and elongated flexible is 10-20 lis long
Rice, light green, the usual a branch of fasciation of 2 pin is on brachyplast.Masson pine is strong positive seeds, likes warm and moist weather, suitable to be born in acid
Property native (pH4.5-6) and well-drained hillside.It is distributed on the south Basin of Huaihe River and Hanshui Valley, west to central Sichuan, expensive
State middle part and southeastern Yunnan, resource very abundant.The grain of wood is straight, and structure is slightly thick, widely builds, sleeper, ore pillar and furniture
Etc. using material；Rosin is medicine, the important source material of chemical industry；Pine stump after felling is usually used in extracting rosin and turpentine oil.Motherland's medicine
The record of useful pine needle treatment disease, modern science worker have verified clear, table to the chemical constituent of pine needle, toxicity research etc.
Bright pine needle is respectively provided with very big potentiality to be exploited in medicine, health care, food and feed etc..
The solvent of Pinus massoniana：Ash content 4.42%, ether extract 9.90%, water extraction 16.80%, tannin
4.43%th, reducing substances 7.06%, pentosan 5.20%, lignin 23.15%, cellulose 19.84%.Domestic and international many
Person studies and points out, the usual 7-16% of gross protein value in pine needle, and finds amino acid at least 17 in contained thick protein
Kind, what wherein content was most has aspartic acid, glutamic acid, leucine, lysine, valine etc..The vitamin contained in pine needle
Compare comprehensively, have vitamin A, B, C, D, E etc., the content of wherein vitamin E is higher (500 mgs/kg).Carrot in pine needle
Cellulose content is also very abundant, up to 197-344 mgs/kg.
For amino acid up to as many as 17 kinds, these amino acid are all the developments of livestock and poultry organism growth in thick protein contained by pine needle
During indispensable nutriment, one of nutriment necessary to contained mineral matter is also livestock and poultry.There is scholar pine needle
In contained carrotene, chlorophyll, vitamin E, steroid and phytocide etc., be collectively referred to as " pine needle biological active matter
Matter ".Through research, this pine needle bioactive substance has growth of animals or poultry obvious promotion as a kind of novel fodder additive
Effect, can improve efficiency of feed utilization, strengthen the disease-resistant rate of livestock and poultry.The pelage quality of weightening, fur-bearing animal to meat animal improves
And the raising of the laying rate, rate of fertilization, incubation rate of poultry has preferable facilitation.Pine needle is processed into pine needle meal, is used
In mixed feed production or be directly used in livestock and poultry daily ration and make additive, be a kind of relatively inexpensive and popular utilization
Approach, China have just succeeded in developing and have tried out in early eighties, and result of the test is good.
The toxicity of pine needle is extremely low, is perfectly safe for human body.Abundant nutrition eats absorption by human body, can promote
The growth and development of human body.Therefore, appropriate technique is taken, the effective ingredient in pine needle and nutriment are reasonably extracted
Come, as food additives, it will rising.
At present, lysozyme is combined with Pinus massoniana for preventing odontopathy, particularly gingivitis and plaque should
With there is not been reported.
The content of the invention
The technical problems to be solved by the invention, which are to provide one kind, can effectively prevent gingivitis and plaque, have no toxic side effect
And drug resistance, the natural feature toothpaste in active ingredient source.
The present invention solves the above problems the technical scheme adopted is that provide a kind of ointment containing Pinus massoniana, wherein
The weight ratio of active ingredient lysozyme and Pinus massoniana extract is (1:10)~(10:1).
Preferably, in the toothpaste, the weight ratio of lysozyme and Pinus massoniana extract is (1:5)~(5:1).
It is furthermore preferred that the weight ratio of lysozyme and Pinus massoniana extract is 1:4.
Preferably, the Pinus massoniana extract is the ethanol extract of Pinus massoniana.
Preferably, the toothpaste further comprises one kind or more in rubbing agent, moisturizer, adhesive, sweetener, water
Kind.
It is furthermore preferred that one or more of the rubbing agent in calcium monohydrogen phosphate, silica etc.；The moisturizer choosing
One or more from sorbierite, propane diols etc.；Described adhesive is selected from carragheen, konjac glucomannan, sodium carboxymethylcellulose etc.
In one or more；The sweetener is saccharin sodium.
Preferably, in the toothpaste of 100 parts by weight, lysozyme and Pinus massoniana extract containing 0.5-5 weight %.
The present invention also provides a kind of application of above-mentioned toothpaste in the toothpaste of prevention gingivitis or plaque is prepared.
The invention has the advantages that：
It is surprising that by repeated tests, present inventors have unexpectedly found that by lysozyme and Chinese herbal medicine Pinus massoniana
Extract combination uses the prevention for gingivitis and plaque to be used with synergistic effect, cooperation other active components
It is better.
Compared with common Chinese herbal toothpaste, toothpaste preparation method of the invention is simple, and compatibility is reasonable, and two kinds of active components exists
While playing respective effect, and synergistic function can be produced, significantly prevent oral cavity gingivitis and plaque.Lysozyme and horse
Tail pine needle source is natural, safe and non-toxic, without side-effects；Due to having no drug resistance in toothpaste without western medicine composition, toothpaste.
Embodiment
The present invention is further described with reference to embodiment, but the implementation of the present invention is not limited to this.Under
State experimental method used in embodiment unless otherwise specified, be conventional method.
The extraction of embodiment 1, Pinus massoniana
After Pinus massoniana dried powder is crossed 40 mesh sieves, with 95% ethanol of 35 times of weight in 60 DEG C of heating and refluxing extractions 3
It is secondary, every time 2 it is small when, merge extracting solution, after concentrate drying to obtain the final product.
The preparation of embodiment 2, lysozyme/Pinus massoniana extract toothpaste
Take 5 parts by weight Pinus massoniana extract as made from embodiment 1, the silica of 50 parts by weight, 20 parts by weight
Sorbierite, the konjac glucomannan of 1 parts by weight, the saccharin sodium of 0.2 parts by weight and the water mixing of surplus, 100 parts by weight are made contains horse
The toothpaste A1 of tail Folium Pini extract.
Take the lysozyme of 5 parts by weight, the silica of 50 parts by weight, the sorbierite of 20 parts by weight, the konjaku of 1 parts by weight
Glue, the saccharin sodium of 0.2 parts by weight and the water mixing of surplus, are made the toothpaste A2 containing lysozyme of 100 parts by weight.
Take the lysozyme of 4.167 parts by weight, the Pinus massoniana extract as made from embodiment 1 of 0.833 parts by weight, 50 weights
The silica of part is measured, the sorbierite of 20 parts by weight, the konjac glucomannan of 1 parts by weight, the saccharin sodium of 0.2 parts by weight and the water of surplus mix
Close, 100 parts by weight are made contains lysozyme and Pinus massoniana extract (weight ratio 5:1) toothpaste A3.
Take the lysozyme of 2.5 parts by weight, the Pinus massoniana extract as made from embodiment 1 of 2.5 parts by weight, 50 parts by weight
Silica, the sorbierite of 20 parts by weight, the konjac glucomannan of 1 parts by weight, the water mixing of the saccharin sodiums of 0.2 parts by weight and surplus,
100 parts by weight are made contains lysozyme and Pinus massoniana extract (weight ratio 1:1) toothpaste A4.
Take the lysozyme of 1 parts by weight, the Pinus massoniana extract as made from embodiment 1 of 4 parts by weight, the two of 50 parts by weight
Silica, the sorbierite of 20 parts by weight, the konjac glucomannan of 1 parts by weight, the saccharin sodium of 0.2 parts by weight and the water mixing of surplus, are made
100 parts by weight contain lysozyme and Pinus massoniana extract (weight ratio 1:4) toothpaste A5.
Take the lysozyme of 0.833 parts by weight, the Pinus massoniana extract as made from embodiment 1 of 4.167 parts by weight, 50 weights
The silica of part is measured, the sorbierite of 20 parts by weight, the konjac glucomannan of 1 parts by weight, the saccharin sodium of 0.2 parts by weight and the water of surplus mix
Close, 100 parts by weight are made contains lysozyme and Pinus massoniana extract (weight ratio 1:5) toothpaste A6.
The application of embodiment 3, the toothpaste comprising lysozyme/Pinus massoniana extract in prevention gingivitis and plaque
3.1 research object
Double blinding, layering, the parallel clinical research carried out to the adult men and women volunteer of health.First from researcher to experiment
Object makes experiment purpose, research details, the responsibility for participating in research clear.After the subject for being ready to participate in fills in voluntary book, by grinding
The person of studying carefully checks it, judges with exclusion standard according to including, screens tested crowd.Preliminary screening process includes soft group of oral cavity
Knit and sclerous tissues checks, oulitis and bacterial plaque inspection are whether being selected in for experimental subjects determined.Finally select improvement Quigley-
210 research objects of Hein plaque index >=1.5 and Loe-Silness index of gingivitis >=1.0 have participated in research, and according to
Plaque index and gingival index are divided into 7 balance groups, and every group of 30 people, men and women is fifty-fifty, and experimental subjects the range of age is 20~40 years old.
3.2 inspection method
Supragingival plaque is evaluated using the Turesky plaque indexs of Quigley-Hein improvement.To supragingival plaque dyeing and root
According to following standard recording：
0=is without bacterial plaque；
The bacterial plaque that 1=necks portion marginal existence is dispersed in；
The visible continuous thin bacterial plaque band (reaching lmm wide) in 2=necks edge；
3=necks portion bacterial plaque band is more than lmm, but less than the 1/3 of facing；
The 1/3~2/3 of 4=bacterial plaques covering facing；
5=bacterial plaques cover facing more than 2/3.
Tooth detection buccal surface and lingual surface, are 10 for the maximum numeration of each tooth.Except third molar, artificial tooth and there is neck reparation
The tooth of body, remaining tooth is in the row of numeration.The mean of each subject's plaque index is succeeded in one's scheme by personal whole tooth detections
The sum of number (each 2 records of tooth) divided by total measurement number (teeth number is multiplied by 2) obtain.
Gingivitis measures gum using improvement Loe-Silness index of gingivitis measurements before dyeing measures plaque index
Situation.Gingivitis method of counting and standard are as follows：
0=is without oulitis；
Oulitis that 1=is slight, gum color and luster and quality are slightly changed, but are visited and examined no bleeding；
2=moderate inflammations, gum are bright.Red, swollen, bleeding is examined in hyperplasia, spy；
3=hyperphlogosises, the obvious rubescent, hyperplasia of gum, have hematostaxis.
As the inspection of plaque index, each tooth inspection record buccal surface and lingual surface, the maximum numeration of a tooth is 6.Remove
Third molar, artificial tooth and the tooth for having neck dummy, remaining tooth are included in the row of numeration.Loe-Silness index of gingivitis
Mean refer to the summation (each 2 face of tooth) divided by total measuring surface number (tooth of measurement multiplies 2) institute's total of all tooth counter values
Value.
3.3 experimental methods and result
After baseline inspection (soft, sclerous tissues, bacterial plaque, gingivitis) is completed, all experimentss object receives to clean one's teeth and according to bacterium
Spot and oulitis situation are divided into 7 balance groups and participate in the research.Random 6 experimental groups being assigned as using 2 toothpaste of embodiment
(A1-A6) and 1 control group (without any active ingredient, the auxiliary material in remaining toothpaste is identical with A1-A6, and excess water complements to 100
Parts by weight).
Subject is using unified soft toothbrush and uses according to regulations toothpaste, it is desirable to brushes teeth within 1st 2 times (early, evenings), every time
1min.It is required that any other dental care product such as other toothpaste or toothbrush, gargle, dental floss are not had in experimentation.It is real
Good balance is carried out before testing from number, age, gender, bacterial plaque and index of gingivitis to experimental subjects to be grouped, nothing between each group
Statistics difference.When using toothpaste 6 months, subject's plaque and gingivitis situation are assessed according to the method described above, 6
Statistical result after a month after clinical examination is shown in Table 1.
The plaque index and index of gingivitis of table each experimental group subject after 16 months
| Plaque index | Index of gingivitis | |
| Control group | 2.70 | 1.95 |
| A1 experimental groups (Pinus massoniana extract) | 2.33 | 1.88 |
| A2 experimental groups (lysozyme) | 2.59 | 1.70 |
| A3 experimental groups (5:1 lysozyme and Pinus massoniana extract) | 2.06 | 1.36 |
| A4 experimental groups (1:1 lysozyme and Pinus massoniana extract) | 1.91 | 1.31 |
| A5 experimental groups (1:4 lysozyme and Pinus massoniana extract) | 1.55 | 0.68 |
| A6 experimental groups (1:5 lysozyme and Pinus massoniana extract) | 1.99 | 1.01 |
The toothpaste of the present invention can effectively prevent gingivitis and plaque it can be seen from the contrast experiment of embodiment 3, special
It is not that lysozyme and being applied in combination for Pinus massoniana extract generate synergy, reduces the usage amount of active ingredient.
In the case that active ingredient total amount remains unchanged, use the toothpaste of lysozyme of the present invention and Pinus massoniana extract (A3-A6)
It is equal compared with the index of gingivitis and plaque index of toothpaste (the A1 and A2) subject that lysozyme or Pinus massoniana extract is used alone
There is different degrees of reduction, it was demonstrated that significant effect of the toothpaste of the present invention in terms of prevention gingivitis and plaque, wherein lysozyme
It is 1 with Pinus massoniana extract weight ratio:4 toothpaste effect is the most prominent, and plaque index and index of gingivitis are only 1.55 respectively
With 0.68, it is significantly higher than control group, generates and be difficult to expected excellent effect.
Claims (5)
1. a kind of toothpaste containing Pinus massoniana, it is characterised in that wherein active ingredient lysozyme and Pinus massoniana extract
Weight ratio is 1:4, the preparation method of the Pinus massoniana extract is as follows：After Pinus massoniana dried powder is crossed 40 mesh sieves, use
95% ethanol of 35 times of weight in 60 DEG C of heating and refluxing extractions 3 times, every time 2 it is small when, merge extracting solution, after concentrate drying to obtain the final product.
2. toothpaste according to claim 1, it is characterised in that toothpaste further comprises rubbing agent, moisturizer, bonding
One or more in agent, sweetener, water.
3. toothpaste according to claim 2, it is characterised in that the rubbing agent is in calcium monohydrogen phosphate, silica
It is one or more；One or more of the moisturizer in sorbierite, propane diols；Described adhesive is selected from carragheen, evil spirit
One or more in taro glue, sodium carboxymethylcellulose；The sweetener is saccharin sodium.
4. the toothpaste according to right wants 1, it is characterised in that in the toothpaste of 100 parts by weight, contain 0.5-5 weight %
Lysozyme and Pinus massoniana extract.
5. application of the claim 1-4 any one of them toothpaste in the toothpaste of prevention gingivitis or plaque is prepared.
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|---|---|---|---|---|
| CN101721324A (en) * | 2010-01-14 | 2010-06-09 | 江苏雪豹日化有限公司 | Biological complex enzyme toothpaste and preparation method thereof |
| CN101822623A (en) * | 2009-03-02 | 2010-09-08 | 孙新龙 | Toothpaste prepared from pine leaves |
| CN103301046A (en) * | 2013-06-09 | 2013-09-18 | 广西奥奇丽股份有限公司 | Chinese herbal medicine toothpaste containing pseudo-ginseng saponin and pine needle extract |
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| JPS5254037A (en) * | 1975-10-28 | 1977-05-02 | Sunstar Inc | Lysozymeeblended dentifrice composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822623A (en) * | 2009-03-02 | 2010-09-08 | 孙新龙 | Toothpaste prepared from pine leaves |
| CN101721324A (en) * | 2010-01-14 | 2010-06-09 | 江苏雪豹日化有限公司 | Biological complex enzyme toothpaste and preparation method thereof |
| CN103301046A (en) * | 2013-06-09 | 2013-09-18 | 广西奥奇丽股份有限公司 | Chinese herbal medicine toothpaste containing pseudo-ginseng saponin and pine needle extract |
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Denomination of invention: A toothpaste containing masson pine leaves Effective date of registration: 20210630 Granted publication date: 20180515 Pledgee: Agricultural Bank of China Limited by Share Ltd. Pujiang County branch Pledgor: ZHEJIANG AEGIS BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. Registration number: Y2021330000759 |