CN110403871B - Composition with blue light resisting and repairing effects and preparation method and application thereof - Google Patents
Composition with blue light resisting and repairing effects and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9741—Pteridophyta [ferns]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
Abstract
The invention discloses a composition with blue light resisting and repairing effects, and a preparation method and application thereof. The composition with the blue light resisting and repairing effects comprises the following components in parts by weight: 1-5 parts of sophora flower seed extract, 20-45 parts of centella asiatica extract and 20-50 parts of selaginella tamariscina extract. The invention also provides a stock solution containing the blue light resisting and repairing composition. Experiments prove that the blue light resisting repair composition can effectively resist the damage of blue light to skin cells, improve the rough skin phenomenon, repair the damaged skin cells, achieve the effects of delaying skin aging and reducing skin pigmentation, has remarkable blue light resisting and repair synergistic effects in three components, is mild in property and high in safety, and does not cause skin irritation.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a composition with blue light resisting and repairing effects, and a preparation method and application thereof.
Background
With the rapid development of modern economy, the living standard of people is improved, and the problem of skin aging, which is the appearance change of wrinkles, skin sagging and pigmentation, is more and more emphasized as the simple substance life is improved to the mental life. The formation of wrinkles and sagging is carried out in a complex form of physiological aging and photoaging. The main cause of skin photoaging is ultraviolet rays, but recently, there is an increasing concern about not only ultraviolet rays but also blue light generated in mobile phones, televisions, various illumination lamps, and the like. Modern people live in a digitalized environment for a longer and longer time when exposed to blue light. Blue light is a blue series of visible light with a wavelength of 380-500 nm, exists in sunlight, is released more in artificial illumination such as computer display screens, TVs and LED lamps, has high penetrability on skin, can damage DNA of cells and slow down recovery speed of skin barriers after long-term exposure, becomes a direct cause of skin aging, causes chloasma and melanin pigmentation, and damages eye health. Blue light is more capable of transmitting light energy to the skin than ultraviolet light, and causes the generation of active oxygen. The existing skin care products have an unsatisfactory effect of preventing blue light.
Disclosure of Invention
The invention aims to make up the defects of the existing blue light resistant and repair products, and provides a composition which is simple in composition, easy to prepare, safe, non-irritating, and good in blue light resistant and repair effects, and a preparation method and application thereof.
The invention is realized by the following technical scheme:
the composition with the blue light resisting and repairing effects comprises the following components in parts by weight:
1-5 parts of sophora flower seed extract
20-45 parts of centella asiatica extract
20-50 parts of selaginella extract.
Preferably, the sophora japonica seed extract is prepared by the following method:
(1) crushing sun-dried sophora japonica seeds, adding the crushed sophora japonica seeds and 95% dealdehyding alcohol into a heating and stirring pot according to the weight ratio of 1: 1-2, heating, refluxing and stirring at 95-100 ℃ for 4-6 hours, cooling to 55-65 ℃ after extraction is completed, filtering to remove sophora japonica seeds, taking filtrate, putting the filtrate into the heating pot again, heating to 85-90 ℃, filtering while hot, performing suction filtration by using a PP cotton filter element, and taking the filtrate for later use;
(2) and (2) putting the filtrate obtained in the step (1) into a heating pot with vacuum, vacuumizing, starting heating, evaporating dealdehydized alcohol at 75-80 ℃, evaporating until no alcohol exists in the heating pot, completing distillation, taking crystals in the pot, and drying to obtain the sophora flower seed extract.
Preferably, the centella asiatica extract is prepared by the following method:
(1) crushing sun-dried centella, adding the crushed centella and 95% dealdehydized alcohol into a heating reflux pot according to the weight ratio of 1: 2-3, heating reflux steaming for 2-3 h at 95-100 ℃, cooling to 45-55 ℃, filtering, and taking filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 60-65 ℃, adding dipropylene glycol with the weight of 1.5-2.5 times that of the centella asiatica when the filtrate is evaporated to leave one fifth of the volume, and uniformly stirring to obtain a mixed solution;
(3) transferring the mixed solution obtained in the step (2) to a rotary evaporator in batches, vacuumizing, and removing the residual dealdehydized alcohol at the temperature of 58-60 ℃ to obtain the centella asiatica extract.
Preferably, the selaginella extract is prepared by the following method:
(1) crushing dried selaginella tamariscina, adding the crushed selaginella tamariscina, butanediol and water into a heating reflux pot according to the weight ratio of 1: 2-3: 1, heating reflux and steaming for 2-3 h at the temperature of 95-100 ℃, cooling to 45-55 ℃, and filtering to obtain filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 80-85 ℃, adding butanediol with the same volume as that in the step (1) when the filtrate is evaporated to leave one third of the volume, and uniformly stirring to obtain a mixed solution;
(3) and (3) adding the mixed solution obtained in the step (2) into a heating reflux pot for reheating, then filtering while the solution is hot, and taking the filtrate to obtain the selaginella tamariscina extract.
The invention also provides application of the composition with the blue light resisting and repairing effects in preparing skin care products or cosmetics with the blue light resisting and repairing effects.
Preferably, the skin care product or cosmetic comprises a product such as an aqueous, lotion or cream.
The invention also provides an anti-blue-light repair stock solution containing the composition with anti-blue-light and repair effects.
Preferably, the anti-blue-light repair stock solution comprises the following components in percentage by mass:
more preferably, the anti-blue light repair stock solution comprises the following components in percentage by mass:
preferably, the polyhydric alcohol is at least one selected from 1, 3-butanediol, glycerol, 1, 3-propanediol, pentanediol and dipropylene glycol.
The invention also provides a method for preparing the blue light resistant repair stock solution, which comprises the following steps:
(1) adding water, polyhydric alcohol, hyaluronic acid, xanthan gum, p-hydroxyacetophenone and hexanediol into a water phase pot, heating to 80-84 ℃, preserving heat, stirring and dissolving uniformly;
(2) and (3) cooling to 40-45 ℃, adding the blue light resisting repair composition and inositol, and uniformly stirring to obtain the blue light resisting repair stock solution.
Compared with the prior art, the invention has the following beneficial effects:
(1) the sophora flower seed extract in the blue light resisting repair composition has the effects of well absorbing blue light, inhibiting the increase of the permeability of skin capillaries, preventing thrombosis, increasing the oxygen content of blood and improving blood vessel microcirculation, the centella extract has the functions of improving and repairing skin barriers, reducing the generation of inflammation media (IL-1 and MMP-1), and also can promote the proliferation of human fibroblasts and the synthesis of human collagen I and 3 to achieve the effect of repairing skin wounds, the selaginella extract has the effects of strongly clearing free radicals in skin, relieving skin aging, repairing damaged skin tissues, promoting wound healing and the like, and the three extracts are combined for application, so that the obvious defect of single function of each extract is overcome, the complementary enhancement effect is also achieved, the blue light resisting property and the oxidation resistance are more obviously improved, the skin care product has the effects of removing free radicals and relieving skin, so that the problems of skin aging, pigmentation and the like caused by blue light can be effectively relieved, damaged skin can be well repaired, and the powerful effect of relieving skin aging is achieved.
(2) The product of the invention does not cause phenomena of allergy, stimulation and the like to users, and is safe to use; the product of the invention has obvious effects on resisting blue light, repairing damaged skin cells and keeping the young and active of skin.
Detailed Description
The following examples are further illustrative of the present invention and are not intended to be limiting thereof. The components of the formulations in the following examples are, unless otherwise specified, conventional commercial products.
Example 1
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
the preparation method comprises the following steps:
(1) adding water, glycerol, hyaluronic acid, xanthan gum, p-hydroxyacetophenone and hexanediol into a water phase pot, heating to 80 ℃, preserving heat, stirring and dissolving uniformly;
(2) cooling to 40 deg.C, adding flos Sophorae Immaturus seed extract, herba Centellae extract, herba Selaginellae extract, and inositol, and stirring to obtain the blue light resisting repairing stock solution.
The sophora flower seed extract, the centella extract and the selaginella extract are prepared as follows:
1. preparing a sophora flower seed extract:
(1) crushing sun-dried sophora japonica seeds, adding the crushed sophora japonica seeds and 95% dealdehyding alcohol into a heating and stirring pot according to the weight ratio of 1:1.5, heating, refluxing and stirring at 98 ℃ for 4 hours, cooling to 60 ℃ after extraction, filtering to remove sophora japonica seeds with 200-mesh filter cloth while hot, taking filtrate, putting the filtrate into the heating pot again, starting heating to 85 ℃, filtering while hot, performing suction filtration with a 0.1mm PP cotton filter element, and taking the filtrate for later use;
(2) putting the filtrate obtained in the step (1) into a heating pot with vacuum, vacuumizing, starting heating, steaming at 78 ℃ to remove aldehyde alcohol until no alcohol exists in the heating pot, completing distillation, taking crystals in the pot, and drying to obtain the sophora flower seed extract.
2. Preparation of centella asiatica extract:
(1) crushing sun-dried centella, adding the crushed centella and 95% dealdehydized alcohol into a stainless steel heating reflux pot according to the weight ratio of 1:2, heating reflux and stewing for 2 hours at 98 ℃, then cooling to 50 ℃, filtering with 200-mesh clean gauze, and taking filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 62 ℃, adding dipropylene glycol with the weight being 2 times that of the herba centellae when the filtrate is evaporated to leave one fifth of the volume, and uniformly stirring to obtain a mixed solution;
(3) transferring the mixed solution obtained in the step (2) to a rotary evaporator in batches, vacuumizing, and removing the residual dealdehydized alcohol at 58 ℃ to obtain the centella asiatica extract.
3. Preparing the selaginella extract:
(1) crushing sun-dried selaginella tamariscina, adding the crushed selaginella tamariscina, butanediol and water into a stainless steel heating reflux pot according to the weight ratio of 1:2:1, heating reflux and stewing for 2 hours at 98 ℃, then cooling to 50 ℃, filtering by using 200-mesh clean gauze, and taking filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 80 ℃, adding butanediol with the same volume as that of the step (1) when the filtrate is evaporated to leave one third of the volume, and uniformly stirring to obtain a mixed solution;
(3) and (3) adding the mixed solution obtained in the step (2) into a heating reflux pot for reheating, then filtering while the solution is hot, and taking the filtrate to obtain the selaginella tamariscina extract.
Example 2
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
the preparation method comprises the following steps:
(1) adding water, glycerol, hyaluronic acid, xanthan gum, p-hydroxyacetophenone and hexanediol into a water phase pot, heating to 84 ℃, preserving heat, stirring and dissolving uniformly;
(2) cooling to 45 deg.C, adding flos Sophorae Immaturus extract, herba Centellae extract, herba Selaginellae extract, and inositol, and stirring to obtain the anti-blue light repairing stock solution.
The sophora flower seed extract, the centella extract and the selaginella extract are prepared as follows:
1. preparing a sophora flower seed extract:
(1) crushing sun-dried sophora japonica seeds, adding the crushed sophora japonica seeds and 95% dealdehydized alcohol into a heating and stirring pot according to the weight ratio of 1:2, heating, refluxing and stirring for 4 hours at 100 ℃, cooling to 55 ℃ after extraction, filtering with 200-mesh filter cloth while hot to remove sophora japonica seeds, taking filtrate, putting the filtrate into the heating pot again, heating to 90 ℃, filtering while hot, performing suction filtration with a 0.1mm PP cotton filter element, and taking the filtrate for later use;
(2) putting the filtrate obtained in the step (1) into a heating pot with vacuum, vacuumizing, starting heating, evaporating dealdehydized alcohol at 75 ℃, evaporating until no alcohol exists in the heating pot, completing distillation, taking crystals in the pot, and drying to obtain the sophora flower seed extract.
2. Preparation of centella asiatica extract:
(1) crushing sun-dried centella, adding the crushed centella and 95% dealdehydized alcohol into a stainless steel heating reflux pot according to the weight ratio of 1:3, heating reflux and stewing for 3 hours at 95 ℃, then cooling to 55 ℃, filtering with 200-mesh clean gauze, and taking filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 65 ℃, adding dipropylene glycol with the weight of 1.5 times that of the centella asiatica when the filtrate is evaporated to leave one fifth of the volume, and uniformly stirring to obtain a mixed solution;
(3) transferring the mixed solution obtained in the step (2) to a rotary evaporator in batches, vacuumizing, and removing the residual dealdehydized alcohol at 60 ℃ to obtain the centella asiatica extract.
3. Preparing the selaginella extract:
(1) crushing sun-dried selaginella tamariscina, adding the crushed selaginella tamariscina, butanediol and water into a stainless steel heating reflux pot according to the weight ratio of 1:3:1, heating reflux and stewing for 3h at 95 ℃, then cooling to 45 ℃, filtering by using 200-mesh clean gauze, and taking filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 85 ℃, adding butanediol with the same volume as that of the step (1) when the filtrate is evaporated to leave one third of the volume, and uniformly stirring to obtain a mixed solution;
(3) and (3) adding the mixed solution obtained in the step (2) into a heating reflux pot for reheating, then filtering while the solution is hot, and taking the filtrate to obtain the selaginella tamariscina extract.
Example 3
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
the preparation method comprises the following steps:
(1) adding water, glycerol, hyaluronic acid, xanthan gum, p-hydroxyacetophenone and hexanediol into a water phase pot, heating to 82 ℃, preserving heat, stirring and dissolving uniformly;
(2) cooling to 42 deg.C, adding flos Sophorae Immaturus extract, herba Centellae extract, herba Selaginellae extract, and inositol, and stirring to obtain the anti-blue light repairing stock solution.
The sophora flower seed extract, the centella extract and the selaginella extract are prepared as follows:
1. preparing a sophora flower seed extract:
(1) crushing sun-dried sophora japonica seeds, adding the crushed sophora japonica seeds and 95% dealdehydized alcohol into a heating and stirring pot according to the weight ratio of 1:1, heating, refluxing and stirring at 95 ℃ for 6 hours, cooling to 65 ℃ after extraction, filtering with 200-mesh filter cloth to remove sophora japonica seeds while hot, taking filtrate, putting the filtrate into the heating pot again, heating to 85 ℃, filtering while hot, performing suction filtration with a 0.1mm PP cotton filter element, and taking the filtrate for later use;
(2) putting the filtrate obtained in the step (1) into a heating pot with vacuum, vacuumizing, starting heating, steaming at 80 ℃ to remove aldehyde alcohol until no alcohol exists in the heating pot, completing distillation, taking crystals in the pot, and drying to obtain the sophora flower seed extract.
2. Preparation of centella asiatica extract:
(1) crushing sun-dried centella, adding the crushed centella and 95% dealdehydized alcohol into a stainless steel heating reflux pot according to the weight ratio of 1:2, heating reflux and stewing for 2 hours at 100 ℃, then cooling to 45 ℃, filtering with 200-mesh clean gauze, and taking filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 60 ℃, adding dipropylene glycol with the weight of 2.5 times that of the centella asiatica when the filtrate is evaporated to leave one fifth of the volume, and uniformly stirring to obtain a mixed solution;
(3) transferring the mixed solution obtained in the step (2) to a rotary evaporator in batches, vacuumizing, and removing the residual dealdehydized alcohol at 58 ℃ to obtain the centella asiatica extract.
3. Preparing the selaginella extract:
(1) crushing sun-dried selaginella tamariscina, adding the crushed selaginella tamariscina, butanediol and water into a stainless steel heating reflux pot according to the weight ratio of 1:2:1, heating reflux and stewing for 2 hours at 100 ℃, then cooling to 55 ℃, filtering by using 200-mesh clean gauze, and taking filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 85 ℃, adding butanediol with the same volume as that of the step (1) when the filtrate is evaporated to leave one third of the volume, and uniformly stirring to obtain a mixed solution;
(3) and (3) adding the mixed solution obtained in the step (2) into a heating reflux pot for reheating, then filtering while the solution is hot, and taking the filtrate to obtain the selaginella tamariscina extract.
Comparative example 1
Comparative example 1 is a blank, stock solution without anti-blue light repair composition, comprising the following components in mass percent:
the preparation process is referred to example 1.
Comparative example 2
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
comparative example 2 blue light-resistant repair stock is compared to example 1, except that the blue light-resistant repair composition contains only sophora japonica seed extract, and is prepared according to example 1.
Comparative example 3
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
comparative example 3 blue light-resistant repair stock is compared with example 1, except that the blue light-resistant repair composition contains only centella asiatica extract, and the preparation method thereof is referred to example 1.
Comparative example 4
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
comparative example 4 blue light-resistant repair stock is compared with example 1 except that the blue light-resistant repair composition contains only selaginella tamariscina extract, and the preparation method thereof is referred to example 1.
Comparative example 5
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
comparative example 4 blue light-resistant repair stock is compared with example 1, except that the blue light-resistant repair composition contains only the sophora flower seed extract and the selaginella tamariscina extract, and the preparation method thereof is as in reference example 1.
Example 4
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
the preparation procedure is as in example 1.
Example 5
The anti-blue light restoration stock solution comprises the following components in percentage by mass:
the preparation procedure is as in example 1.
First, physicochemical test
The physical and chemical tests of the blue light-resistant repair stock solutions prepared in examples 1 to 3 and comparative examples 1 to 5 were carried out, respectively, and the results were as follows:
1. appearance: the stock solution of comparative example 1 was a colorless transparent viscous liquid, and the remaining stock solution was a yellowish transparent viscous liquid.
2. pH value: the stock solutions of examples 1-3 and comparative examples 1-5 all had a pH range between 5.5 and 7.5.
3. Heat resistance stability test (48 ℃ C.. times.72 h): all stock solutions were qualified for heat stability.
4. Cold resistance stability test (-15 ℃ C.. times.72 h): cold resistance stability all stock solutions were acceptable.
Second, safety and efficacy testing
(1) Testing the human body safety patch:
the experimental steps are as follows: the selected area is not more than 50mm2And a qualified patch device with the depth of about 1mm, wherein a patch test is carried out on the back of 30 volunteers, the stock solution samples prepared in examples 1-3 and comparative examples 1-5 and a blank control sample (purified water) are respectively placed on the patch device in sequence, 0.02mL of the samples of examples 1-3 and comparative examples 1-5 is selected, the purified water is placed in a control hole to serve as a blank control sample, then a low-sensitization adhesive tape is used for being attached to the back of each tester for testing, and the test is carried out by lightly pressing the palm of the hand to be uniformly attached to the skin for 24 hours.
The experimental results are as follows: after the test is carried out for 24 hours, 30 minutes (after the pressure mark disappears) after the spot pasting device on the back of the subject is removed, the back skin of the subject is observed to have no red or stabbing pain phenomenon or suspected erythema reaction, and the result shows that the stock solution containing the blue light resisting repair composition is safe and has no adverse reaction phenomenon.
(2) Evaluation test of blue light resistance:
test samples: stock samples prepared in examples 1-3 and comparative examples 1-5, incubators with blue light source, culture medium containing human keratinocytes, opsin-3 fluorescent marker, light microscope.
The experimental steps are as follows: 8 culture mediums containing human keratinocytes are selected and numbered respectively, then the same amount of stock solution samples prepared in examples 1-3 and comparative examples 1-5 are added in sequence, the same amount of opsin (opsin-3) fluorescent marker is added, finally the mixture is placed in an incubator with a blue light source for culturing for 24 hours, the culture medium is taken out after 24 hours, and the density of fluorescence in the culture medium is observed on a microscope.
The experimental results are as follows: the fluorescence intensity of the culture medium of the stock solution samples of examples 1-3 is reduced in sequence, and the fluorescence density of example 1 is the maximum and occupies 93 percent of the area of the culture medium; the fluorescence density of the medium of example 2 was 73%; the fluorescence density of the medium of example 3 was 65%; the fluorescence density range of comparative example 1 was the smallest, there was no fluorescence range at all, and the fluorescence density was 0%; the fluorescence density of the medium of comparative example 2 was 52%; the fluorescence densities of the media of comparative example 3 and comparative example 4 were very close, about 2%, and the fluorescence density was very weak, and the fluorescence density of the media of comparative example 5 was 59%. Since human keratinocytes secrete a protein called opsin (opsin-3) and decrease or even do not secrete the protein under the irradiation of blue light, if the added sample has no efficacy of resisting blue light, the opsin (opsin-3) is decreased or even not, and the protein can be completely seen by a fluorescence labeling method. From the above results, the blue light resisting efficacy of the stock solution samples in examples 1 to 3 is obviously better than that of the stock solution samples in comparative examples 1 to 5, and the blue light resisting efficacy of the samples in examples 1 to 3 shows a trend of being weaker and weaker with the decrease of the concentration of the sophora japonica seed extract, so that the blue light resisting repair composition provided by the invention has a synergistic blue light resisting repair effect by the combined application of the sophora japonica seed extract, the centella asiatica extract and the selaginella tamariscina extract.
(3) Lactic acid sting repair efficacy test:
test samples: stock samples prepared in examples 1-3 and comparative examples 1-5, 10% v/v lactic acid solution.
Subject: 8 healthy skin volunteers between 30 and 45 years of age were selected.
The experimental steps are as follows: selecting 8 volunteers, respectively coating 10% lactic acid solution on one side of each arm to stimulate, so as to cause the skin of the arm to have red and stabbing pain, and then respectively using the samples of examples 1-3 and comparative examples 1-5 to repair the stimulated skin, wherein the corresponding volunteer 1 uses the stock solution sample of example 1, the corresponding volunteer 2 uses the stock solution sample of example 2, the corresponding volunteer 3 uses the stock solution sample of example 3, the corresponding volunteer 4 uses the stock solution sample of comparative example 1, the corresponding volunteer 5 uses the stock solution sample of comparative example 2, the corresponding volunteer 6 uses the stock solution sample of comparative example 3, the corresponding volunteer 7 uses the stock solution sample of comparative example 4, and the corresponding volunteer 8 uses the stock solution sample of comparative example 5.
Recording time: the skin repair of arm skin was recorded for 1h, 3h, 7h, and 12h, respectively.
The experimental results are as follows:
TABLE 1 lactic acid sting repair results
As can be seen from table 1, the stock solutions of examples 1 to 3 had a reduced skin-repairing effect with a reduced amount of the anti-blue light repairing composition, so that the stock solutions of example 1 had the best skin-repairing effect. Comparative example 1 does not contain the blue light-resistant repair composition and has no effect on skin repair, comparative example 2 only contains the sophora japonica seed extract and has poor skin repair effect, comparative examples 3-4 respectively only contain the centella asiatica extract and the selaginella tamariscina extract and have reduced skin repair effect, and comparative example 5 contains the sophora japonica seed extract and the selaginella tamariscina extract and has better skin repair effect than comparative examples 3-4 but still weaker than example 3, so that the sophora japonica seed extract, the centella asiatica extract and the selaginella tamariscina extract in the blue light-resistant repair composition synergistically exert good skin repair and blue light-resistant effects.
In conclusion, the blue light resisting and repairing stock solution containing the blue light resisting repairing composition is safe and low in irritation, and can effectively resist the damage of blue light to skin cells, improve the phenomenon of rough skin, repair damaged skin cells and achieve the effects of delaying skin aging and reducing skin pigmentation after being frequently used.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (3)
1. The blue light resisting repair stock solution is characterized by comprising the following components in percentage by mass:
the blue light resistant repair composition comprises the following components in parts by weight:
1-5 parts of sophora flower seed extract
20-45 parts of centella asiatica extract
20-50 parts of selaginella extract;
the sophora flower seed extract is prepared by the following method:
(1) crushing sun-dried sophora japonica seeds, adding the crushed sophora japonica seeds and 95% dealdehyding alcohol into a heating and stirring pot according to the weight ratio of 1: 1-2, heating, refluxing and stirring at 95-100 ℃ for 4-6 hours, cooling to 55-65 ℃ after extraction is completed, filtering to remove sophora japonica seeds, taking filtrate, putting the filtrate into the heating pot again, heating to 85-90 ℃, filtering while hot, performing suction filtration by using a PP cotton filter element, and taking the filtrate for later use;
(2) putting the filtrate obtained in the step (1) into a heating pot with vacuum, vacuumizing, starting heating, evaporating dealdehydized alcohol at 75-80 ℃, evaporating until no alcohol exists in the heating pot, namely completing distillation, taking crystals in the pot, and drying to obtain the sophora flower seed extract;
the centella asiatica extract is prepared by the following method:
(1) crushing sun-dried centella, adding the crushed centella and 95% dealdehydized alcohol into a heating reflux pot according to the weight ratio of 1: 2-3, heating reflux steaming for 2-3 h at 95-100 ℃, cooling to 45-55 ℃, filtering, and taking filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 60-65 ℃, adding dipropylene glycol with the weight of 1.5-2.5 times that of the centella asiatica when the filtrate is evaporated to leave one fifth of the volume, and uniformly stirring to obtain a mixed solution;
(3) transferring the mixed solution obtained in the step (2) to a rotary evaporator in batches, vacuumizing, and removing the residual dealdehydized alcohol at the temperature of 58-60 ℃ to obtain the centella asiatica extract;
the selaginella extract is prepared by the following method:
(1) crushing dried selaginella tamariscina, adding the crushed selaginella tamariscina, butanediol and water into a heating reflux pot according to the weight ratio of 1: 2-3: 1, heating reflux and steaming for 2-3 h at the temperature of 95-100 ℃, cooling to 45-55 ℃, and filtering to obtain filtrate;
(2) transferring the filtrate obtained in the step (1) into a rotary evaporator, vacuumizing, distilling at 80-85 ℃, adding butanediol with the same volume as that in the step (1) when the filtrate is evaporated to leave one third of the volume, and uniformly stirring to obtain a mixed solution;
(3) adding the mixed solution obtained in the step (2) into a heating reflux pot for reheating, then filtering while the solution is hot, and taking the filtrate to obtain the selaginella tamariscina extract;
the polyalcohol is at least one selected from 1, 3-butanediol, glycerol, 1, 3-propanediol, pentanediol and dipropylene glycol.
2. The anti-blue-light repair stock solution according to claim 1, comprising the following components in percentage by mass:
3. a method of preparing the anti-blue-light repair stock solution of claim 1 or 2, comprising the steps of:
(1) adding water, polyhydric alcohol, hyaluronic acid, xanthan gum, p-hydroxyacetophenone and hexanediol into a water phase pot, heating to 80-84 ℃, preserving heat, stirring and dissolving uniformly;
(2) and (3) cooling to 40-45 ℃, adding the blue light resisting repair composition and inositol, and uniformly stirring to obtain the blue light resisting repair stock solution.
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