CN110393297A - A kind of nanoemulsions and preparation method thereof improving apocarya oil-proofness - Google Patents
A kind of nanoemulsions and preparation method thereof improving apocarya oil-proofness Download PDFInfo
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- 239000007908 nanoemulsion Substances 0.000 title claims abstract description 73
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
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- 238000000034 method Methods 0.000 claims abstract description 13
- 239000004064 cosurfactant Substances 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 238000004945 emulsification Methods 0.000 claims abstract description 5
- 108010073771 Soybean Proteins Proteins 0.000 claims description 58
- 235000019710 soybean protein Nutrition 0.000 claims description 58
- 239000001814 pectin Substances 0.000 claims description 43
- 229920001277 pectin Polymers 0.000 claims description 43
- 235000010987 pectin Nutrition 0.000 claims description 31
- 235000013339 cereals Nutrition 0.000 claims description 19
- 239000012071 phase Substances 0.000 claims description 19
- 244000068988 Glycine max Species 0.000 claims description 7
- 235000010469 Glycine max Nutrition 0.000 claims description 7
- 238000006703 hydration reaction Methods 0.000 claims description 6
- 230000036571 hydration Effects 0.000 claims description 5
- 239000008055 phosphate buffer solution Substances 0.000 claims description 5
- 239000008346 aqueous phase Substances 0.000 claims description 3
- 230000001804 emulsifying effect Effects 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 235000020195 rice milk Nutrition 0.000 claims description 2
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- 238000005516 engineering process Methods 0.000 abstract description 4
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- 241000723418 Carya Species 0.000 description 1
- 244000068645 Carya illinoensis Species 0.000 description 1
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- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 241000899834 Obovaria olivaria Species 0.000 description 1
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- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
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- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000010470 pecan oil Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
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- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 239000008170 walnut oil Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
- A61K8/068—Microemulsions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/412—Microsized, i.e. having sizes between 0.1 and 100 microns
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/413—Nanosized, i.e. having sizes below 100 nm
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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- Food Science & Technology (AREA)
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- Oil, Petroleum & Natural Gas (AREA)
- Dispersion Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Colloid Chemistry (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of nanoemulsions and preparation method thereof for improving apocarya oil-proofness, belong to food processing technology field.The nanoemulsions include apocarya oil, emulsifier, cosurfactant and water, and the average grain diameter of nanoemulsions is 40~200nm；Preparation method includes the mixing of emulsifier, the preparation of thick lotion, ultrasonic emulsification into nanoemulsions.Oil-in-water type nano-emulsion liquid prepared by the present invention, physical stability with higher, good storage-stable, for apocarya oil storage and anti-oxidant propose new method.
Description
Technical field
The present invention relates to food processing technology field, more particularly to a kind of raising apocarya oil-proofness
Nanoemulsions and preparation method thereof.
Background technique
Apocarya category Juglandaceae hickory, green fruit of trade name, source area are northern Mexico and the U.S., In
The hickory nut product of commercial type has walnut drink, walnut powder, walnut oil etc..
Apocarya kernel oil content is extremely abundant, and the unsaturated fatty acid in grease is as unhealthful factor
One of, content accounts for 90% of total fatty acids or more, and linolenic acid (omega-fatty acid) and linoleic acid (ω -6 rouge in grease
Fat acid) ratio close to golden ratio 1:4.Research shows that apocarya oil keeps fit and healthy, moisturizes the skin, beautifying face and moistering lotion,
Brain tonic supports brain, prevention cardiovascular and cerebrovascular disease, improves the effects of endocrine, but oil does not dissolve in water for apocarya, illumination condition
Under it is unstable, be easily oxidized, largely limit apocarya oil in the application of food industry.
Therefore, how a kind of raising apocarya oil-proofness is provided and water-soluble nanoemulsions are art technologies
The problem of personnel's urgent need to resolve.
Summary of the invention
In view of this, the present invention will be thin the present invention provides a kind of nanoemulsions for improving apocarya oil-proofness
Shell pecan oil is prepared into emulsion, can enhance its stability and water solubility, and then can extend the shelf life of oil product, expands
Big application range.
In order to achieve the above object, the present invention adopts the following technical scheme:
A kind of preparation method for the nanoemulsions improving apocarya oil-proofness, comprising the following steps:
(1) soybean protein isolate and pectin are dissolved in phosphate buffer solution respectively, sufficiently take soybean separation protein after hydration
White and pectin mixed solution is as water phase；
(2) apocarya oil is uniformly mixed with cosurfactant as oily phase；
(3) during the oil phase is added to the aqueous phase, it is stirred emulsifying；
(4) ultrasonic emulsification is carried out with ultrasonic cell disruptor in ice bath, is improved apocarya oil-proofness
Nanoemulsions.
Preferably, it is above-mentioned it is a kind of improve apocarya oil-proofness nanoemulsions preparation method in, step (1)
Described in the mass concentration of soybean protein isolate and the pectin be 0.2~0.7%, and the soybean protein isolate and institute
The volume ratio for stating pectin is 1:1~1:3, is further preferably 1:1.
Preferably, it is above-mentioned it is a kind of improve apocarya oil-proofness nanoemulsions preparation method in, step (1)
Described in phosphate buffer solution concentration be 0.1mol/L, so that pH value is remained 3.
Preferably, it is above-mentioned it is a kind of improve apocarya oil-proofness nanoemulsions preparation method in, step (1)
Middle soybean protein isolate and pectin are dissolved in respectively in phosphate buffer solution is stirred 4-6 hours, at a temperature of 4-12 DEG C sufficiently
It is hydrated, takes out solution after 12-14h, prevent soybean protein isolate and pectin solution from precipitating, be put into and stir 1-2h at room temperature, take big
The mixed liquor of beans protein isolate and pectin is as water phase.
The beneficial effect of above-mentioned technical proposal is: since the hydration of soybean protein isolate solution is very slow, if at normal temperature into
Guild's cooperating microorganisms cause solution rotten, and hydration can eliminate the influence of microorganism and temperature to solution under low temperature.
Preferably, it is above-mentioned it is a kind of improve apocarya oil-proofness nanoemulsions preparation method in, step (2)
Described in the volume ratio of apocarya oil and the cosurfactant be 1:1~3:1, further preferred 2:1, and it is described
Surfactant is Tween 80.
The beneficial effect of above-mentioned technical proposal is: if increasing ratio shared by apocarya oil, will lead to partial size increasing
Greatly, for particle size range not within the scope of nanoemulsions, aforementioned proportion restriction can keep partial size in suitable range.
Preferably, it is above-mentioned it is a kind of improve apocarya oil-proofness nanoemulsions preparation method in, step (3)
In, mutually the concentration in the water phase is 0.2-0.6% to the oil.
The beneficial effect of above-mentioned technical proposal is: if oily phase concentration is too high, emulsifier cannot fully wrapped around oil droplet, if
Concentration is too low, will lead to emulsifier surplus, partial size is caused to increase, and above-mentioned concentration range may insure that oil is mutually completely wrapped.
Preferably, it is above-mentioned it is a kind of improve apocarya oil-proofness nanoemulsions preparation method in, step (3)
Described in mixing speed be 10000 ± 1000r/min, mixing time be 2 ± 1min.
Preferably, it is above-mentioned it is a kind of improve apocarya oil-proofness nanoemulsions preparation method in, step (4)
Described in the ultrasonic power of ultrasonic emulsification be 570W, time 20min, basic setup is that ultrasound is opened and stopped within 5 seconds 5 seconds.
The beneficial effect of above-mentioned technical proposal is: if ultrasonic procedure continuous operations can generate a large amount of heat, higher temperature
Degree will will lead to soybean protein isolate and go bad, while influence the quality of oil, therefore ultrasound should carry out in ice bath, and control super
Sound process is opened stops 5 seconds for 5 seconds, it is ensured that under lower energy consumption, improves product quality.
The invention also discloses a kind of nanometers of raising apocarya oil-proofness being prepared by the above method
Lotion, the average grain diameter of the nanoemulsions for improving apocarya oil-proofness are 40-200nm.
It can be seen via above technical scheme that compared with prior art, the present disclosure provides a kind of raising shell mountains
The partial size of the preparation method of the nanoemulsions of walnut oil-proofness, obtained nanoemulsions dispersion particle is small, by gravity
It is small, it is not easy to coalesce, and Brownian movement is significant, therefore higher stability can be kept, nanoemulsions have higher
Penetrability, function factor can directly by skin absorb, increase operation rate, nanoemulsions can be used for substituting liposome and
After birth becomes new function factor vectors.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The embodiment of invention for those of ordinary skill in the art without creative efforts, can also basis
The attached drawing of offer obtains other attached drawings.
Fig. 1 attached drawing is the change of size curve of soybean protein isolate-pectin, soybean protein isolate nanoemulsions at 4 DEG C；
Fig. 2 attached drawing is that the change of size of soybean protein isolate-pectin, soybean protein isolate nanoemulsions at 37 DEG C is bent
Line；
Fig. 3 attached drawing is that the change of size of soybean protein isolate-pectin, soybean protein isolate nanoemulsions at 55 DEG C is bent
Line；
Fig. 4 attached drawing is that the change of size of soybean protein isolate-pectin, soybean protein isolate nanoemulsions at 80 DEG C is bent
Line；
Fig. 5 attached drawing is the transmission electron microscopy on soybean protein isolate (left side), soybean protein isolate-pectin (right side) nanoemulsions
Mirror image；
Fig. 6 attached drawing is the volume ratio of apocarya oil and cosurfactant tween to the shadow of apocarya oiliness energy
Ring diagram；
Fig. 7 attached drawing is influence diagram of the pH value to apocarya oil nano emulsions；
Fig. 8 attached drawing is the influence diagram of soybean protein isolate concentration (SPI) to apocarya oil nano emulsions；
Fig. 9 attached drawing is the influence diagram of concentration of pectin (HMP) to apocarya oil nano emulsions；
Figure 10 attached drawing is influence diagram of the ultrasonic power to apocarya oil nano emulsions.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The present invention provides a kind of nanoemulsions for improving apocarya oil-proofness, the present invention is oily by apocarya
It is prepared into emulsion, its stability and water solubility can be enhanced, and then the shelf life of oil product can be extended, expands and applies model
It encloses.
Embodiment 1
A certain amount of soybean protein isolate and pectin (± 0.001g) are accurately weighed in the pH=3 phosphate buffer of 0.1M
(PBS) 6h is stirred in respectively, is put at 4 DEG C overnight, is sufficiently hydrated, taken out after 12h, stir 2h at room temperature, taking volume ratio is 1:1
Soybean protein isolate and liquid pectin as water phase, take tween that volume ratio is 1:2 and oil after mixing evenly, be added slowly to
In aqueous phase solution, thick lotion is formed using high-speed shearing machine 10000r/min homogeneous 3min, it is then thin with ultrasonic wave in ice bath
Born of the same parents' pulverizer ultrasound 2 times, each 10min, basic setup is opened for ultrasound to stop 5 seconds for 5 seconds, prepares nanoemulsions.
The average grain diameter of apocarya oil nano emulsions manufactured in the present embodiment is 47.89nm.
Prepare four groups of apocarya oil nano emulsions at identical conditions, first group is placed in 4 DEG C of refrigerator and stores up
30 days, hiding, the one group of data (partial size) of measurement in every 7 days, the constant temperature and humidity that second group and third component are not placed on 37 DEG C and 55 DEG C are trained
It supports in case, carries out a sample measurement every 30min, the 4th group is placed in 80 DEG C of water-baths, carries out a sample every 15min
Product measurement, measurement result is referring to table 1-4, and by attached drawing 1-4 it is found that apocarya oil nano emulsions are stored under the conditions of 4 DEG C
Stability is best, under the conditions of 37 DEG C, 55 DEG C and 80 DEG C, and partial size increases with the increase of storage time.
The hydrophobicity of soybean protein isolate is stronger, and pectin, Arabic gum are that a kind of hydrophily is very strong and hydrophobicity is insufficient
Polysaccharide, it is therefore desirable to the collaboration of both protein-PSs uses, and the addition of polysaccharide can not only reduce interfacial tension, but also can
To prevent droplet coalescence, improve emulsion intercalation method, nanoemulsions feature image is observed by transmission electron microscope it is found that
Only the drop of the lotion of soybean protein isolate is in ball and size distribution unevenness, and aggregation is obvious, and Fig. 5 can be seen that interfacial film is too thin
Have the tendency that fusion, and be added to the lotion of pectin, drop is dispersed in continuous phase, soybean consistent with conclusion (of pressure testing)
Protein isolate-pectin multiple emulsion is more preferable than single soybean protein isolate emulsion-stabilizing effect.
Comparative example 1
A certain amount of soybean protein isolate (± 0.001g) is accurately weighed in the pH=3 phosphate buffer (PBS) of 0.1M
It stirs 6 hours, is put at 4 DEG C overnight respectively, sufficiently after hydration, taken out after 12h, stir 2h at room temperature, taking volume ratio is 1:1's
Soybean protein isolate and pectin take tween that volume ratio is 1:2 and oil after mixing evenly, are added slowly to mix as water phase
In solution, it is initially formed thick lotion using high-speed shearing machine 10000r/min homogeneous 3min, supersonic cell is then used in ice bath
Pulverizer, ultrasound 2 times, each 10min, basic setup is opened for ultrasound to stop 5 seconds for 5 seconds, prepares nanoemulsions.
The average grain diameter of the apocarya oil nano emulsions of this comparative example preparation is 53.26nm.
Prepare four groups of apocarya oil nano emulsions under the same conditions, first group is placed in 4 DEG C of refrigerator and stores
30 days, every 7d measured one group of data (partial size), and second group and third component are not placed on 37 DEG C and 55 DEG C of constant temperature and humidity culture
It is primary every 30min measurement sample in case, lotion is placed in 80 DEG C of water-bath, it is primary every 15min measurement sample,
Measurement result is referring to table 1-4.
14 DEG C of measurement results of table
| 0d | 7d | 14d | 21d | 28d | |
| Soybean protein isolate-pectin lotion | 47.89nm | 49.31nm | 49.90nm | 52.38nm | 54.89nm |
| Soybean protein isolate lotion | 53.26nm | 57.83nm | 63.42nm | 68.89nm | 76.54nm |
2 37 DEG C of measurement results of table
| 0min | 30min | 60min | 90min | 120min | |
| Soybean protein isolate-pectin lotion | 47.89nm | 58.35nm | 63.44nm | 73.50nm | 85.30nm |
| Soybean protein isolate lotion | 53.26nm | 75.00nm | 97.00nm | 104.00nm | 119.00nm |
3 55 DEG C of measurement results of table
| 0min | 15min | 30min | 45min | 60min | |
| Soybean protein isolate-pectin lotion | 47.89nm | 63.24nm | 69.80nm | 78.30nm | 90.56nm |
| Soybean protein isolate lotion | 53.26nm | 79.50nm | 103.00nm | 128.00nm | 145.00nm |
4 80 DEG C of measurement results of table
| 80℃ | 0min | 20min | 40min | 60min | 80min |
| Soybean protein isolate-pectin lotion | 47.89nm | 80.34nm | 97.50nm | 99.30nm | 105.00nm |
| Soybean protein isolate lotion | 53.26nm | 108.00nm | 125.00nm | 141.00nm | 168.00nm |
In conjunction with attached drawing 1-4 it is found that apocarya oil nano emulsions store under the conditions of 4 DEG C, 37 DEG C, 55 DEG C and 80 DEG C,
At 4 DEG C, nanoemulsions place that 30d change of size is not significant, and the middle and later periods tends towards stability, and partial size is presented at 37 DEG C, 55 DEG C, 80 DEG C
Linear upward trend.The main reason for causing nanoemulsions unstable is Ostwald ripening, the side that emulsion system is reduced to free energy
To evolution, and the increase of average grain diameter is then coalescence and flocculated result.This shows soybean protein isolate-polysaccharide composite lotion
There is certain stabilization to high temperature, increases emulsion intercalation method.
Specifically, the present invention is respectively from the volume ratio of apocarya oil and cosurfactant, pH value, soybean separation protein
Four white concentration, concentration of pectin and ultrasonic power aspects have carried out related experiment, nano-emulsion to apocarya oil nano emulsions
Liquid generally refers to transparent or semitransparent system of the average grain diameter integrated distribution between 50~200nm, particle diameter distribution index (PDI)
Indicating particle diameter distribution situation in emulsion system, particle diameter distribution index value is smaller, illustrate that particle size distribution range is smaller in emulsion system,
Droplet distribution is better, and system is more stable.
(1) influence of the volume ratio of apocarya oil and cosurfactant tween to apocarya oiliness energy
The preparation method is the same as that of Example 1 for the nanoemulsions of raising apocarya oil-proofness, passes through and changes thin shell mountain pecan
The ratio of peach oil and cosurfactant is respectively 1:1,2:1,3:1,4:1,5:1, and measurement apocarya oil is lived with surface is helped
Influence of the ratio of property agent to apocarya oil nano emulsions average grain diameter.
From fig. 6 it can be seen that when influence of the pH value to apocarya oiliness energy changes apocarya oil and helps table
When the ratio of face activating agent is 1-3:1, the average grain diameter integrated distribution of lotion is nanoemulsions in 50~200nm.
(2) influence of the pH value to apocarya oiliness energy
The preparation method is the same as that of Example 1 for the nanoemulsions of raising apocarya oil-proofness, passes through and changes pH value difference
It is 3,5,7,9 and 11, measures pH value to apocarya oil nano emulsions average grain diameter, particle diameter distribution index (a), Zeta-potential
(b), the influence of turbidity (c) and encapsulation rate (d), as a result referring to Fig. 7, the apocarya being prepared under the conditions of pH=5 is oily
The average grain diameter of nanoemulsions is 198.36nm, and the particle diameter distribution index of lotion is 0.503, turbidity 72.03cm-1, Zeta-potential
For -3.31mV.
Since soybean protein isolate is isoelectric point in pH=4.5 or so, solubility is minimum, and the soybean protein isolate quantity of electric charge connects
Nearly 0, interparticle electrostatic repulsion is also very low, so that the associative ability between pectin is most weak, forms the ability decline of emulsion,
And flocculation or polymerization easily occur at this time, show as emulsion particle diameter increase；However when pH deviates isoelectric point, charged drop
Repulsive force is greater than the Van der Waals force to attract each other, will not assemble between drop, emulsion stabilization.In pH=3, soybean separation protein
It is white positively charged below isoelectric point, and pH value of solution be lower than pectin (Pka=3.8 or so) when, degree of ionization is suppressed, pectin
Anionic group (carboxylic group) can attract each other with albumen, it is compound that here it is electrostatic, this process will lead under turbidity
Drop, partial size reduce, and Zeta-potential is positive, and absolute value is very big, exist so the complex compound that soybean protein isolate-pectin is formed is stablized
The lotion obtained when pH=3 is best.
(3) influence of the soybean protein isolate concentration to apocarya oiliness energy
The preparation method is the same as that of Example 1 for the nanoemulsions of raising apocarya oil-proofness, passes through and changes soybean separation
Protein concentration is respectively 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, measures soybean protein isolate concentration
To apocarya oil nano emulsions average grain diameter, particle diameter distribution index (a), Zeta-potential (b), turbidity (c) and encapsulation rate (d)
It influences, as a result referring to Fig. 8.
As shown in figure 8, with soybean protein isolate concentration from 0.1% rise to 0.3% when, average grain diameter is decreased obviously,
This is because soybean protein isolate concentration is not enough to wrap up oil droplets, soybean protein isolate adsorbs multiple oil droplets to form bridge
Connection is easy to happen the drop that aggregation generates greater particle size, as the increase of soybean protein isolate concentration is realized to oil droplets
Saturation absorption, to prevent from assembling, when soybean protein isolate concentration increases to 0.3%, average grain diameter reaches 43.74nm,
Particle diameter distribution index is again smaller than 0.3, encapsulation rate 95.8%.When soybean protein isolate concentration continues growing, nanoemulsions are put down
Equal partial size is consequently increased, but variation is not significant, and particle diameter distribution index is consequently increased, this is because when soybean protein isolate is dense
When spending big, unadsorbed protein molecule causes emptying in continuous phase, and drop is caused to flocculate.
In addition, the absolute value of emulsion intercalation method and Zeta-potential has certain relationship, Zeta-potential absolute value is bigger, and lotion more becomes
In stabilization.Water phase pH is lower than isoelectric points of proteins (pI), and protein solution is positively charged, and water phase pH is higher than isoelectric points of proteins
(PI), protein solution is negatively charged, and pectin is a kind of anion polysaccharide, and ionization is reversible, the ionization master in water phase
If the carboxylic group of esterification, when the pKa (about 3.8) of pectin is greater than pH value of solution, ionization is suppressed, under the electrically charged amount of institute
Drop, soybean protein isolate and the pectin surface when pH is 7.0 are negatively charged, therefore lotion Zeta-potential is negative value.Soybean separation
Protein concentration influences lotion Zeta-potential unobvious.
The turbidity of lotion also reaction system stability to a certain extent, as soybean protein isolate concentration rises from 0.1%
To 0.3%, lotion turbidity reduction to 47.26cm-1, research shows that emulsion particle diameter is shadow when the oil phase volume in lotion is fixed
The principal element of turbidity is rung, the two, which is presented, to be positively correlated, and lotion average grain diameter is bigger, and system turbidity is higher, and partial size and turbidity change
As a result it is consistent.When soybean protein isolate concentration is greater than 0.3%, turbidity increases as soybean protein isolate concentration increases
Add, this may be because interfacial protein reaches saturation, and unadsorbed protein content is excessively high to be caused.
According to soybean protein isolate concentration to lotion average grain diameter, particle diameter distribution index, Zeta-potential, turbidity, embedding rate
Influence it can be concluded that, building apocarya oil nano emulsions most suitable soybean protein isolate concentration be 0.3%.
(4) influence of the concentration of pectin to apocarya oiliness energy
The preparation method is the same as that of Example 1 for the nanoemulsions of raising apocarya oil-proofness, passes through and changes concentration of pectin
Respectively 0,0.1%, 0.2%, 0.3%, 0.4%, 0.5% measure soybean protein isolate concentration to apocarya oil nanometer
The influence of lotion average grain diameter, particle diameter distribution index (a), Zeta-potential (b), turbidity (c) and encapsulation rate (d), as a result referring to Fig. 9.
The hydrophobicity of soybean protein isolate is stronger, and pectin is that a kind of hydrophily is very strong and the insufficient polysaccharide of hydrophobicity, because
This, which needs to cooperate with, uses, and the addition of polysaccharide, which can reduce interfacial tension, can also prevent droplet coalescence, improves emulsion intercalation method,
As shown in figure 9, average grain diameter 43.74nm, particle diameter distribution index reaches 0.263 when the concentration of pectin increases to 0.2%,
Zeta-potential is -24.38mV, illustrates that system is in stable state, but with the increase of pectin, partial size, turbidity and the partial size of system
Profile exponent rises, this is because concentration of pectin increases and association between soybean protein is limited and leads to extra fruit
Glue, which is dispersed in lotion, causes turbidity to rise, and the variation of other indexs is unobvious, illustrates that concentration of pectin is compound with albumen at 0.2%
It is best.
(5) influence of the ultrasonic power to apocarya oiliness energy
The preparation method is the same as that of Example 1 for the nanoemulsions of raising apocarya oil-proofness, passes through and changes ultrasonic power
It is 40%, 50%, 60%, the 70% of rated power (rated power is 950W), measurement ultrasonic power receives apocarya oil
Rice milk liquid average grain diameter, particle diameter distribution index (a), Zeta-potential (b), the influence of turbidity (c) and encapsulation rate (d), as a result referring to figure
10。
Power from the 40% of rated power rise to 60% when, lotion average grain diameter drops to 48.23nm from 65.97nm
Drop to 0.264 from 0.383 with particle diameter distribution index.For rated power 40% when, nanoemulsions partial size 60nm with
On, and particle diameter distribution index is larger, it may be possible to power is too small, when keeping particle size dispersion in system uneven, however rising to 60%, greatly
Beans protein isolate-pectin compound emulsifying agent can prepare that partial size is smaller, nanoemulsions of the particle diameter distribution index less than 0.3, and
When rising to 70%, partial size and particle diameter distribution index rise 20.55nm and 0.116 again respectively, this is because power is excessive,
Make albuminous degeneration, causes lotion unstable.The variation of Zeta-potential absolute value is unobvious, it is contemplated that energy consumption and the quality of production are asked
Topic, selects rated power 60% as the power for preparing apocarya oil nano emulsions.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so being described relatively simple, related place is said referring to method part
It is bright.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (9)
1. a kind of preparation method for the nanoemulsions for improving apocarya oil-proofness, which comprises the following steps:
(1) soybean protein isolate and pectin are dissolved in phosphate buffer solution respectively, sufficiently hydration after take soybean protein isolate and
The mixed solution of pectin is as water phase；
(2) apocarya oil is uniformly mixed with cosurfactant as oily phase；
(3) during the oil phase is added to the aqueous phase, it is stirred emulsifying；
(4) ultrasonic emulsification is carried out with ultrasonic cell disruptor in ice bath, is improved receiving for apocarya oil-proofness
Rice milk liquid.
2. the preparation method of the nanoemulsions according to claim 1 for improving apocarya oil-proofness, feature exist
In the mass concentration of soybean protein isolate described in step (1) and the pectin is 0.2~0.7%, and the soybean point
It is 1:1~1:3 from the volume ratio of albumen and the pectin.
3. the preparation method of the nanoemulsions according to claim 1 for improving apocarya oil-proofness, feature exist
In the concentration of phosphate buffer solution described in step (1) is 0.1~0.5mol/L.
4. the preparation method of the nanoemulsions according to claim 1 for improving apocarya oil-proofness, feature exist
In, hydration reaction described in step (1) 4~12 DEG C at a temperature of carry out.
5. the preparation method of the nanoemulsions according to claim 1 for improving apocarya oil-proofness, feature exist
In the volume ratio of apocarya oil described in step (2) and the cosurfactant is 1:1~3:1.
6. the preparation method of the nanoemulsions according to claim 1 for improving apocarya oil-proofness, feature exist
In in step (3), mutually the concentration in the water phase is 0.2~0.6% to the oil.
7. the preparation method of the nanoemulsions according to claim 1 for improving apocarya oil-proofness, feature exist
In mixing speed described in step (3) is 10000 ± 1000r/min, and mixing time is 2 ± 1min.
8. the preparation method of the nanoemulsions according to claim 1 for improving apocarya oil-proofness, feature exist
In the ultrasonic power of ultrasonic emulsification described in step (4) is 570W, time 20min.
9. the nano-emulsion for the raising apocarya oil-proofness that a kind of any one of claim 1-8 the method is prepared
Liquid, which is characterized in that the average grain diameter of the nanoemulsions for improving apocarya oil-proofness is 40-200nm.
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| CN102178240A (en) * | 2011-04-20 | 2011-09-14 | 华宝食用香精香料（上海）有限公司 | Method for preparing microemulsion of walnut oil |
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